CN109122589A - It pupates outside Agasicles hygrophila stem based on humid control device and experimental method - Google Patents
It pupates outside Agasicles hygrophila stem based on humid control device and experimental method Download PDFInfo
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- 241000134126 Agasicles hygrophila Species 0.000 title claims abstract description 40
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- 238000012360 testing method Methods 0.000 claims description 33
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 30
- 230000001850 reproductive effect Effects 0.000 claims description 26
- 230000004083 survival effect Effects 0.000 claims description 16
- 239000012153 distilled water Substances 0.000 claims description 15
- 235000011187 glycerol Nutrition 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 244000026873 Alternanthera philoxeroides Species 0.000 claims description 13
- 230000032669 eclosion Effects 0.000 claims description 10
- 241000382353 Pupa Species 0.000 claims description 9
- 230000019617 pupation Effects 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
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- 229920001817 Agar Polymers 0.000 description 1
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- 241000428811 Alternanthera Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
It pupates outside the present invention relates to a kind of Agasicles hygrophila stem based on humid control device and experimental method, belong to technical field of insect feeding, specifically include pupate tank and cage of pupating, tank of pupating is made of organic glass material, the inner wall lower of tank of pupating is provided with annular protrusion, multiple cages of pupating, which are placed on, pupates in tank, and bottom is supported on annular protrusion, it pupates being provided centrally with through through-hole of cage, the screw top of tank of pupating is connected with top cover, funnel is provided in the middle part of top cover, funnel and top cover integrally connected, filler cap is provided on funnel, a venthole is provided on filler cap, lower portion passes through the bottom that tank of pupating is extended to through through-hole, the present invention can facilitate, accurately determine the relationship pupated between ambient humidity outside Agasicles hygrophila stem, it is more advantageous to the popularization pupated outside Agasicles hygrophila stem, using.
Description
Technical field
It pupates outside the present invention relates to a kind of Agasicles hygrophila stem based on humid control device and experimental method, belongs to elder brother
Worm breeding technology field.
Background technique
Alternanthera philoxeroides (Alternanthera philoxeroides) it is a kind of global malignant weed, by China
It is classified as three kinds of important one of Alien Invasive Plants.Alternanthera philoxeroides has been diffused into 20 Yu Ge provinces and cities of China, autonomous region, happiness drought
Alternanthera not only influences agriculture, forestry, animal husbandry and fishery, but also does great damage to the bio-diversity on invasion ground, the ecosystem, and China is every
Year economic loss as caused by Alternanthera philoxeroides is up to 600,000,000 RMB.Agasicles hygrophila (Agasicles hygrophila),
Belong to coleoptera Chrysomelidae, is the special feeding habits natural enemy insect of Alternanthera philoxeroides.Due to its good control efficiency, successively by the U.S.,
The country such as Australia, China introduces, and has important biological control application value.The thermal adaptability of Agasicles hygrophila compared with
Difference, many areas need artificial propagation to carry out field supplement release and overwintering protection to maintain its Weed control effect.Under natural conditions,
The mature larva of Agasicles hygrophila, which needs to pierce, to be completed to pupate in the stalk of host plant Alternanthera philoxeroides, is in addition pupated to happiness
The stalk of yerbadetajo seed grass also has certain requirement, and reaching its corresponding standard could ensure that it is pupated and eclosion rate.Indoor raising
In the process, feeding worm of mowing is needed, especially not only stalk is required, but also wants since pupa time required time is longer in pupa time
Keep host plant fresh, otherwise plant be easy it is withered and yellow rot, seriously affect and pupate and eclosion rate.
At home and abroad there are 2 documents to be reported about research of pupating outside the stem of Agasicles hygrophila at present.First text
(breeding technology pre-test Chinese biological of pupating outside the Agasicles hygrophila such as Chen Lei stem prevention and treatment, 2009,25 (S1): 6-8.) are offered to grind
Study carefully and shows that wet sandy loam can be used as matrix of pupating and pupate outside stem.(gold opens the straight chest of the such as peace lotus grass to second document
The optimization Agriculture of Anhui science for condition of pupating outside flea beetle stem, 2015,43(12): 111-112,135) by screening sandy loam,
Three kinds of flower mud, agar matrix of pupating find the flower mud matrix of pupating preferably as Agasicles hygrophila.
The large-scale production of Agasicles hygrophila is successfully the important prerequisite of biological control Alternanthera philoxeroides, however due to lotus
Careless straight chest flea beetle is to pupate in stem, and test matrix needs to carry out before use more cumbersome professional method processing, seriously
It has fettered its large-scale production and the marketization is promoted.Therefore, how to be guaranteed by technology of pupating outside stem and be improved pupa time survival rate,
Simplifying operation is the key that Agasicles hygrophila large-scale production and the marketization are promoted.Accordingly, we are to Agasicles hygrophila
The relationship pupated between method and ambient humidity outside stem is studied.
Summary of the invention
To solve technical problem of the existing technology, the present invention provides a kind of, and the straight chest of lotus grass based on humid control is jumped
It pupates outside first stem device and experimental method, can conveniently, accurately determine and pupate outside Agasicles hygrophila stem and ambient humidity
Between relationship, be more advantageous to the popularization pupated outside Agasicles hygrophila stem, application.
To achieve the above object, the technical scheme adopted by the invention is as follows outside the Agasicles hygrophila stem based on humid control
Pupate experimental provision, including pupate tank and cage of pupating, the tank of pupating using the production of organic glass material, the tank of pupating it is interior
Wall lower part is provided with annular protrusion, and multiple cages of pupating, which are placed on, pupates in tank, and bottom is supported on annular protrusion, described
It pupates being provided centrally with through through-hole of cage, the screw top of the tank of pupating is connected with top cover, the middle part setting of the top cover
There are funnel, the funnel and top cover integrally connected, be provided with filler cap on the funnel, one is provided on the filler cap and is led to
Stomata, the lower portion pass through the bottom that tank of pupating is extended to through through-hole.
Preferably, the cage of pupating is stacked, bottommost pupate height of the cage away from tank bottom be 150mm, top
Distance of the pupa cage away from top cover is 50mm.
Preferably, the cage of pupating uses aperture to make for the stainless steel mesh of 60 mesh, and cage top of pupating portion is provided with opening,
And it is provided with sealing net in opening, the sealing Netcom, which crosses velcro and is fixed on, pupates on cage.
It pupates outside Agasicles hygrophila stem based on humid control experimental method, including above-mentioned experimental provision, according to following
Step is operated,
A, the configuration of solution and the control of humidity are matched according to different volumes using glycerine and distilled water, pass through different bodies
Product proportion realizes the control of different humidity, by taking relative humidity RH is respectively 70%, 80%, 90%, 100% as an example, i.e. relative humidity RH
When being 70%, mixed using 1295 ml glycerine and 702 ml distilled water；When relative humidity RH is 80%, 1108 are utilized
Ml glycerine and 936 ml distilled water are mixed；When relative humidity RH is 90%, 718 ml glycerine and 1435 ml are utilized
Distilled water is mixed；When relative humidity RH is 100%, distilled water 2340ml, glycerine is mixed for 0；
B, it tests, it is five test groups of pupating in 70%, 80%, 90%, 100% and stalk that experiment, which is divided into relative humidity RH, each
Test group for examination ovum amount be 150-180, be placed in 25 DEG C, relative humidity 80 ± 5%, 14 L:10 D of periodicity of illumination feeding worm
It raises in room, every larva is respectively placed in foster worm bottle after egg hatching, fresh Alternanthera philoxeroides blade is provided daily,
The quantity of observation daily and record ovum, larva；When mature larva will pupate, entered to pupate in cage and stalk, then will be changed
Pupa cage, which is put into, pupates in tank, and tank of pupating then is added by funnel in the solution that relative humidity RH is 70%, 80%, 90%, 100%
It is interior, the amount of solution is added by weight of the volume for tank of pupating carries out, the relative humidity RH for the tank that makes to pupate reaches 70%, 80%, 90% and
100%；Timing observation daily is pupated situation later, identify after insect sprouts wings and statistics male and female after be put in foster worm bottle raise respectively
It supports, for mating and laying eggs, replaces fresh Alternanthera philoxeroides for each foster worm bottle daily, and observe and record the survival of female male worm
And egg laying amount, until all adults are dead；
C, data are analyzed, and are write and are imported program bag for all initial data for collecting record, utilize computer program TWOSEX-
MSChart carries out data analysis, analyzes the hatching rate of Agasicles hygrophila, the life such as percentage of pupation, eclosion rate, service life and reproductive capacity
And parameter and population, design parameter include given age stage survival rates xj , given age survival ratel x , the female given age stage
Reproductive capacityf x4, given age reproductive capacitym x With the net reproductive capacity of given agel x m x It include intrinsic rate of increase with parameter and populationr, week
Limit growth rateλ, net reproductive rateR 0And mean generation timeT, all data obey bootstrap method, using 100,000 pumpings
Then sample carries out the significance of difference by using paired bootstrap test again to estimate the standard error of parameters
Analysis.
Compared with prior art, the present invention has following technical effect that the present invention by Agasicles hygrophila by being placed in
Tank of pupating is interior pupate outside stem, then by each humidity pupated in tank of control respectively, makes Agasicles hygrophila different
It is raised under humidity environment, observes and records the life parameters such as every group of percentage of pupation, eclosion rate, breeding potential and service life and population
Parameter is compared pupating in above-mentioned parameter and stem, determines the optimum humidity proportion pupated outside Agasicles hygrophila stem, determines
It pupates and is not significantly different in the Agasicles hygrophila and stem raised under humidity environment, can guarantee and improve pupa time in this way
Survival rate, simplified operation, making to pupate outside Agasicles hygrophila stem is able to carry out large-scale production and marketization popularization.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the comparison diagram of different humidity test group Agasicles hygrophila different developmental phases survival rate in the present invention.
Fig. 3 be the present invention in different humidity test group Agasicles hygrophila given age survival rate (l x );Female is specific
Age level reproductive capacity (f x4);Given age reproductive capacity (m x ) and the net reproductive capacity of given age (l x m x ) comparison diagram.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Accompanying drawings and embodiments are closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only
To explain the present invention, it is not intended to limit the present invention.
As shown in Figure 1, experimental provision of pupating outside the Agasicles hygrophila stem based on humid control, including pupate tank 1 and change
Pupa cage 2, tank 1 of pupating are made of organic glass material, and the inner wall lower for tank 1 of pupating is provided with annular protrusion 3, multiple cages of pupating
2 are placed on and pupate in tank 1, and bottom is supported on annular protrusion 3, and cage 2 of pupating is provided centrally with through through-hole 4, tank 1 of pupating
Screw top be connected with top cover 5, the middle part of top cover 5 is provided with funnel 6, funnel 6 and 5 integrally connected of top cover, is arranged on funnel 6
There is filler cap 7, a venthole is provided on filler cap 7,6 lower part of funnel passes through the bottom for extending to tank 1 of pupating through through-hole 4
Portion.
Wherein, cage 2 of pupating is stacked, bottommost pupate height of the cage away from tank bottom be 150mm, top pupate cage away from
The distance of top cover is 50mm.Cage 2 of pupating uses aperture to make for the stainless steel mesh of 60 mesh, and cage top of pupating portion is provided with opening,
And sealing net is provided in opening, sealing Netcom, which crosses velcro and is fixed on, pupates on cage.
In in the present invention, (1) tank shape of pupating is a cylindrical body (300 × 500 mm of φ), by high grade of transparency acrylic board
It is commonly called as the production of organic glass (PMMA) material, with a thickness of 3 mm.(2) tank top of pupating is a lid, this lid is threaded, can be with
Rotation is covered and is pupated on tank in cylindrical body, and a funnel is connected on lid, be with lid it is integrated, the diameter of filler tube is 10 mm.
(3) there is a caping on funnel, when injecting liquid into tank of pupating, caping can be opened, usually caping covers on funnel,
There is the aperture of 3 mm on caping, the effect of aperture is to guarantee pupate tank and external air currents.(4) tank skin is pupated from lower part
Start to retract 5 mm from outside to inside at 150 mm, shelves inner ring as cage of pupating.(5) cage of pupating uses the stainless steel of 60 mesh
Screen making, 295 mm of diameter, 150 mm of height open the square osculum of one 20 × 20 mm above cage of pupating, for will
The mature larva access pupated is pupated cage, all after access, velcro can be used and seal, and when use opens velcro.(6)
The mature larva pupated is accessed into cage of pupating outside tank of pupating, the cage that then will pupate is put into tank of pupating, and is placed in shelving
At circle, then similarly second cage of pupating is placed on first cage of pupating.So far, two cages of pupating have used 300 mm
The space of height, lower part is there are the space of 150 mm height, and there are the spaces of 50 mm height on top.(7) cage center of pupating is stayed
There is the hole of 10 mm, is passed through for the filler tube that pipe covers, 450 mm of funnel pipe range.
When being tested, operation is followed the steps below,
A, the configuration of solution and the control of humidity are matched according to different volumes using glycerine and distilled water, pass through different bodies
Product proportion realizes the control of different humidity, by taking relative humidity RH is respectively 70%, 80%, 90%, 100% as an example, i.e. relative humidity RH
When being 70%, mixed using 1295 ml glycerine and 702 ml distilled water；When relative humidity RH is 80%, 1108 are utilized
Ml glycerine and 936 ml distilled water are mixed；When relative humidity RH is 90%, 718 ml glycerine and 1435 ml are utilized
Distilled water is mixed；When relative humidity RH is 100%, distilled water 2340ml, glycerine is mixed for 0；
B, it tests, it is five test groups of pupating in 70%, 80%, 90%, 100% and stalk that experiment, which is divided into relative humidity RH, each
Test group for examination ovum amount be 150-180, be placed in 25 DEG C, relative humidity 80 ± 5%, 14 L:10 D of periodicity of illumination feeding worm
It raises in room, every larva is respectively placed in foster worm bottle after egg hatching, fresh Alternanthera philoxeroides blade is provided daily,
The quantity of observation daily and record ovum, larva；When mature larva will pupate, entered to pupate in cage and stalk, then will be changed
Pupa cage, which is put into, pupates in tank, and tank of pupating then is added by funnel in the solution that relative humidity RH is 70%, 80%, 90%, 100%
It is interior, the amount of solution is added by weight of the volume for tank of pupating carries out, the relative humidity RH for the tank that makes to pupate reaches 70%, 80%, 90% and
100%；Timing observation daily is pupated situation later, identify after insect sprouts wings and statistics male and female after be put in foster worm bottle raise respectively
It supports, for mating and laying eggs, replaces fresh Alternanthera philoxeroides for each foster worm bottle daily, and observe and record the survival of female male worm
And egg laying amount, until all adults are dead；
C, data are analyzed, and are write and are imported program bag for all initial data for collecting record, utilize computer program TWOSEX-
MSChart carries out data analysis, analyzes the hatching rate of Agasicles hygrophila, the life such as percentage of pupation, eclosion rate, service life and reproductive capacity
And parameter and population, design parameter include given age stage survival rates xj , given age survival ratel x , the female given age stage
Reproductive capacityf x4, given age reproductive capacitym x With the net reproductive capacity of given agel x m x It include intrinsic rate of increase with parameter and populationr, week
Limit growth rateλ, net reproductive rateR 0And mean generation timeT, all data obey bootstrap method, using 100,000 pumpings
Then sample carries out the significance of difference by using paired bootstrap test again to estimate the standard error of parameters
Analysis.
As shown in Fig. 2, recording its different development rank in life daily in the analysis of stage at age-- gender population structure
Section survival rate (s xj ), and determine the gender of adult.Compared to other humidity measurement groups, test group is pupated all in 100% RH and stem
Higher percentage of pupation and eclosion rate are shown, there was no significant difference for the two (D and E in figure).70% RH test group do not sprout wings out at
Worm, percentage of pupation and eclosion rate are 0.80% RH test group is also only sprouted wings 4 female adults without reproductive capacity out, percentage of pupation 30%, plumage
Rate is also 33%.The percentage of pupation and eclosion rate of 90% RH test group are also significantly lower than in stem test group of pupating (figure A, B and C).Cause
This, percentage of pupation and eclosion rate are remarkably decreased with the reduction of humidity.From the point of view of bulk life time (ovum to adult death), 100% RH
The service life pupated in test group and stem, there was no significant difference, other humidity service life significantly shorten.
As shown in figure 3, the survival rate of Agasicles hygrophila different phases xj It is combined as age-specific
survival rate (l x ),l x The existence history of entire population is summarized.The female given age stage reproductive capacity (f x4: It is female
Property adult was the 4th stage), given age reproductive capacity (m x ) and the net reproductive capacity of given age (l x m x ) shown in Fig. 3, daily
Many kinds of parameters is recorded until all individuals are all dead.70% RH and 80% RH test group can lay eggs out due to not sprouting wings
Female adult, so total reproductive capacity is 0.When other 3 test groups compare with control group,l x It all shows dynamically to decline,
In the decline of 90% RH test group it is the most obvious (A in figure).Althoughm x Value be generally lower thanf x4, butm x And f x4Curve have it is similar
Dynamic rule.Agasicles hygrophilam x And f x4Data show there is phase between control group of pupating in 100% RH and stem
As variation tendency (B and C in figure), but in 90% RH test group, it is observed that different oviposition modes (A in figure).?
It pupates in 100% RH and stem, the peak value of reproductive capacity appears in the age 35 ~ 40 days or so, breeds in entire aging course later
Power is gradually reduced, and breeding period is to terminate for 100 days or so at the age.And in 90% RH test group, reproductive capacity is decreased obviously, breeding period
Also terminated at 44 days.In addition, the net reproductive capacity of the given age of Agasicles hygrophila (l x m x ) control of pupating in 100% RH and stem
Group higher (B and C in figure), but in 90% RH test group, which remains very low, it can only observe limited progeny size,
Breeding period withm x And f x4Unanimously, also it is obviously shortened (A in figure).
Agasicles hygrophila under different humidity, list in table 1 by parameter and population of pupating outside stem.For measuring Population breeding
The most important parameter and population of power first is that net reproduction rate (R 0), individual can produce the sum of offspring during representing its life.
Statistics indicate that 90% RH test group net reproduction rate (R 0 =11.9) it is substantially less than 100% RH(R 0 =165.4), 100% RH
With control group of pupating in stem (R 0 =202.5) test group is not significantly different.In addition, also calculate intrinsic rate of increase (r), week limits
Growth rate (λ), these values indicate the theoretical growth rate of the Agasicles hygrophila population under stable age level.It is surveyed in 90% RH
Under examination grouprValue is 0.0630 day-1, the value be substantially less than in stem and pupate (r = 0.1295 day-1).It is similar, λ's
1.1382 day that value is also pupated out of control stem-1Drop to 1.0651 day of 90% RH test group-1.And 100% RH is tested
GrouprValue (r = 0.1196 day-1) andλValue (λ =1.1270 day-1) be not significantly different with pupating in stem.Average generation
Also there is same rule for time T, 90% RH test group T is 37.64 days, with (41.13) and 100% RH(42.54 of pupating in stem)
Significant difference, both rear then difference is not significant.
The parameter and population (mean ± SE) of the Agasicles hygrophila of 1. different humidity test group of table
| Parameter and population | 90% RH | 100% RH | CK |
| Net reproduction rate (R 0) | 11.9±5.0 b | 165.4±34.0 a | 202.5±42.2 a |
| Intrinsic rate of increase (r, day-1) | 0.0630±0.0133 b | 0.1196±0.0051 a | 0.1295±0.0052 a |
| Finite rate of increase (λ, day-1) | 1.0651±0.0141 b | 1.1270±0.0057 a | 1.1382±0.0059 a |
| Mean generation time (T, day) | 37.64±0.49 b | 42.54±0.59 a | 41.13±0.56 a |
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Made any modifications, equivalent replacements, and improvements etc. within principle, should all wrap within the scope of the present invention.
Claims (4)
1. experimental provision of pupating outside the Agasicles hygrophila stem based on humid control, including tank of pupating, with cage of pupating, feature exists
In: the tank of pupating is made of organic glass material, and the inner wall lower of the tank of pupating is provided with annular protrusion, multiple described
Cage of pupating, which is placed on, pupates in tank, and bottom is supported on annular protrusion, and the cage of pupating is provided centrally with through through-hole, institute
The screw top for stating tank of pupating is connected with top cover, and funnel, the funnel and top cover integrally connected are provided in the middle part of the top cover,
It is provided with filler cap on the funnel, a venthole is provided on the filler cap, the lower portion, which passes through, runs through through-hole
Extend to the bottom for tank of pupating.
2. experimental provision of pupating outside the Agasicles hygrophila stem according to claim 1 based on humid control, feature exist
In: the cage of pupating is stacked, and bottommost pupates height of the cage away from tank bottom for 150mm, and top pupates cage away from top cover
Distance is 50mm.
3. experimental provision of pupating outside the Agasicles hygrophila stem according to claim 1 based on humid control, feature exist
In: the cage of pupating uses aperture to make for the stainless steel mesh of 60 mesh, and cage top of pupating portion is provided with opening, and is arranged in opening
There is sealing net, the sealing Netcom, which crosses velcro and is fixed on, pupates on cage.
4. pupate outside the Agasicles hygrophila stem based on humid control experimental method, including above-mentioned experimental provision, which is characterized in that
Operation is followed the steps below,
A, the configuration of solution and the control of humidity are matched according to different volumes using glycerine and distilled water, pass through different bodies
Product proportion realizes the control of different humidity, by taking relative humidity RH is respectively 70%, 80%, 90%, 100% as an example, i.e. relative humidity RH
When being 70%, mixed using 1295 ml glycerine and 702 ml distilled water；When relative humidity RH is 80%, 1108 are utilized
Ml glycerine and 936 ml distilled water are mixed；When relative humidity RH is 90%, 718 ml glycerine and 1435 ml are utilized
Distilled water is mixed；When relative humidity RH is 100%, distilled water 2340ml, glycerine is mixed for 0；
B, it tests, it is five test groups of pupating in 70%, 80%, 90%, 100% and stalk that experiment, which is divided into relative humidity RH, each
Test group for examination ovum amount be 150-180, be placed in 25 DEG C, relative humidity 80 ± 5%, 14 L:10 D of periodicity of illumination feeding worm
It raises in room, every larva is respectively placed in foster worm bottle after egg hatching, fresh Alternanthera philoxeroides blade is provided daily,
The quantity of observation daily and record ovum, larva；When mature larva will pupate, entered to pupate in cage and stalk, then will be changed
Pupa cage, which is put into, pupates in tank, and tank of pupating then is added by funnel in the solution that relative humidity RH is 70%, 80%, 90%, 100%
It is interior, the amount of solution is added by weight of the volume for tank of pupating carries out, the relative humidity RH for the tank that makes to pupate reaches 70%, 80%, 90% and
100%；Timing observation daily is pupated situation later, identify after insect sprouts wings and statistics male and female after be put in foster worm bottle raise respectively
It supports, for mating and laying eggs, replaces fresh Alternanthera philoxeroides for each foster worm bottle daily, and observe and record the survival of female male worm
And egg laying amount, until all adults are dead；
C, data are analyzed, and are write and are imported program bag for all initial data for collecting record, utilize computer program TWOSEX-
MSChart carries out data analysis, analyzes the hatching rate of Agasicles hygrophila, the life such as percentage of pupation, eclosion rate, service life and reproductive capacity
And parameter and population, design parameter include given age stage survival rates xj , given age survival ratel x , the female given age stage
Reproductive capacityf x4, given age reproductive capacitym x With the net reproductive capacity of given agel x m x It include intrinsic rate of increase with parameter and populationr, week
Limit growth rateλ, net reproductive rateR 0And mean generation timeT, all data obey bootstrap method, using 100,000 pumpings
Then sample carries out the significance of difference by using paired bootstrap test again to estimate the standard error of parameters
Analysis.
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| CN114403098A (en) * | 2021-12-22 | 2022-04-29 | 中国农业科学院农业基因组研究所 | Lotus herb direct chest flea beetle pupating device and pupating method thereof |
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| CN102499188A (en) * | 2011-11-16 | 2012-06-20 | 山西农业大学 | Agasicles hygrophila experimental population raising method |
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