CN106093369B - Method for evaluating safety of the Bt paddy rice to non-target insects brown paddy plant hopper - Google Patents
Method for evaluating safety of the Bt paddy rice to non-target insects brown paddy plant hopper Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The present invention relates to a kind of method for evaluating safety of Bt paddy rice to non-target insects brown paddy plant hopper.This method is studied the ecological grade of fit of brown paddy plant hopper by age at age both sexes life table, so as to illustrate ecological security risk of the Bt paddy rice to brown paddy plant hopper；Specifically include five parts：（1）Development duration and life-span；（2）Age at age survival rate；（3）Fecundity and parameter and population；（4）Life-span expectation and reproduction value；（5）Age distribution and Population forecast；Pass through the ecological risk of the comparative analysis overall merit Bt paddy rice of five aspects to non-target insects brown paddy plant hopper.The present invention establishes the evaluation method of the non-target effect of system evaluation Bt paddy rice in laboratory using two-sex population life table, studies and determine that the ecological security of Bt Rice Croppings is significant to improving influence of the Bt paddy rice to non-target organism.
Description
Technical field
The invention belongs to genetically modified crops ecological safety risk assessment technical field, especially a kind of Bt paddy rice is to non-target
The method for evaluating safety of insect brown paddy plant hopper.
Background technology
Paddy rice is the important cereal crops of China, grain-production in occupation of vital strategic position (Xia Rubing,
2009), while paddy rice is also the cereal crops (Zhang Wenhui and Liu Guangjie, 2001) of insect pest most serious.In recent years due to global gas
Time warms, and the lepidoptera pest such as striped rice borer, yellow rice borer, pink rice borer and rice leaf roller is increasingly serious to the degree that causes harm of paddy rice, gives
Peasant bring serious economic loss (Han Yong is strong etc., 2008；Cloud, 1996 are built in Lei Huizhi and river).Utilize technique for gene engineering
The Bt transgenic rice of cultivation can effectively prevent and treat lepidoptera pest, and its Bt toxalbumin expressed has high to target pest
Resistance (Cai Wanlun etc., 2008；Xiao Hanxiang etc., 2011；Xu Xueliang etc., 2013).However, the missing of target pest ecological niche, is
It is no to cause the rising of non-target insects (leafhopper class, plant hopper etc.) population density, so as to be changed into Main Harmful by minor pests
Worm (Lu et al, 2010).Up to now, existing more than the 20 country plantation genetically modified crops (James, 2015) in the whole world.To be true
Protect genetically modified crops safe handling, for non-target organism ecological safety risk assessment be it is necessary (Yu et al,
2011)。
Brown paddy plant hopper [Nilaparvata lugens ()], belong to Semiptera Delphacidae, be a kind of secondary on the paddy rice of Asia
Monophagy insect, the water that rice strain bast is mainly directly drawn by sucking mouth parts causes harm paddy rice, and can pass
Broadcast Virus Diseases of Rice (Wang Rongfu etc., 1998).Because the major target object of the Bt albumen of Bt transgenic rice expression is squama wing
Mesh insect, brown paddy plant hopper belongs to non-target insects.Life table is always the important tool for studying Entomological Ecology.But tradition female
Age is overlapping, male is not considered and not comprising the precocious stage death rate, so as to make what is obtained due to ignoring for age life table
There is deviation in parameter and population, it is impossible to the actual dynamic of definite reflection insect population (Chi and Liu, 1985；Huang and
Chi,2012；Wu Kunjun etc., 1994).In order to improve and supplement the not enough, imperfect of traditional life table, Chi and Liu (1985)
Both sexes Theory of Life Table is proposed, and be used widely in genecology research (Li et al, 2015；Tuan et
al,2016；Wang et al,2016；Yin et al,2013).
The content of the invention
It is an object of the invention to provide a kind of Bt paddy rice to the method for evaluating safety of non-target insects brown paddy plant hopper, the party
Method by build brown paddy plant hopper Bt paddy rice and control non-transgenic paddy rice on experimental population age-age both sexes life table, from
Study genecology angle system Bt paddy rice non-target insects brown paddy plant hopper is grown, the influence of life-span and breeding,
Aim to provide a kind of safety evaluatio side of system, accurate, easy, science transgenic paddy rice to non-target insects brown paddy plant hopper
Method.
To achieve the above object, the technical scheme is that：A kind of safety of Bt paddy rice to non-target insects brown paddy plant hopper
Property evaluation method, comprises the following steps,
S1：Calculate and record brown paddy plant hopper Bt paddy rice and its control non-transgenic paddy rice on include development duration, the life-span with
And the parameter of fecundity；
S2：Calculate age-age survival rate of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S3：Calculate fecundity and population dynamic parameter of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S4：Calculate life-span expectation and reproduction value of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S5：Analyze stable age-age distribution and population of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice
Prediction.
In an embodiment of the present invention, Bt paddy rice selection turns cry1Ab gene pest-resistant paddy rice mf-MH3301-1, described
It is control parent non-transgenic paddy rice MH3301 to compare non-transgenic paddy rice.
In an embodiment of the present invention, it is that brown paddy plant hopper is calculated by Bootstrap methods in Bt water in the step S1
Include the parameter of development duration, life-span and fecundity on rice and its control non-transgenic paddy rice.
In an embodiment of the present invention, the S2-S5 acceptable ages-age both sexes life-table method, it is specific to pass through year
Age-age both sexes life table computer program Twosex-MS Chart carry out analysis age of acquisition-age to step S1 recording parameterses
Phase survival rate, fecundity, population dynamic parameter, life-span expectation, reproduction value, stable age-age distribution and Population forecast.
In an embodiment of the present invention, population dynamic of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice
Parameter calculation formula is as follows：
λ=er；GRR=Σ mx
Wherein, n is the full age number of brown paddy plant hopper, and x and i are the age, and j is age；lxRefer to whole population survival to the general of age x
Rate；SxjRefer to new hatchlings survival to the probability of x ages at age j；mxRefer to eggs on average amount of the population in age x；fxjRefer to it is female into
Eggs on average amount of the worm in age x；R0For net breeding rate；R is intrinsic rate of increase；λ is finite rate of increase；T is Generation means week
Phase；GRR is total reproductive capacity GRR；RxFor age characteristics proliferation rate.
In an embodiment of the present invention, brown paddy plant hopper single head in teat glass is raised, teat glass diameter 3cm height
20cm。
Compared to prior art, the invention has the advantages that：The present invention passes through Bootstrap (n=100000)
Method calculates the standard error of the development duration of brown paddy plant hopper, life-span, fecundity, population quantity, and population dynamic parameter etc.
(SE), and by program Twosex-MS Chart paired bootstrap test functions compare and take food two kinds of paddy rice
The difference of brown paddy plant hopper development duration, reproduction, life-span, parameter and population etc..
Brief description of the drawings
Fig. 1 is the inventive method flow chart.
Fig. 2 is age-age Survival curves (s of the brown paddy plant hopper on Bt paddy rice (A) and non-transgenic paddy rice (B)xj)。
Fig. 3 is the population age feature survival rate (l of the brown paddy plant hopper on Bt paddy rice (A) and non-transgenic paddy rice (B)x), year
Age-age feature fecundity (fxj), population age feature fecundity (mx), population age feature relative reproductive value (lxmx)。
Fig. 4 is life-span expectation (e of the brown paddy plant hopper on Bt paddy rice (A) and non-transgenic paddy rice (B)xj)。
Fig. 5 is reproduction value (v of the brown paddy plant hopper on Bt paddy rice (A) and non-transgenic paddy rice (B)xj)。
Fig. 6 is stable age distribution of the brown paddy plant hopper on Bt paddy rice (A) and non-transgenic paddy rice (B).
Fig. 7 is Population Size prediction of the brown paddy plant hopper on Bt paddy rice (A) and non-transgenic paddy rice (B).
Embodiment
Below in conjunction with the accompanying drawings, technical scheme is specifically described.
As shown in figure 1, a kind of method for evaluating safety of the Bt paddy rice of the present invention to non-target insects brown paddy plant hopper, including such as
Lower step,
S1：Calculate and record brown paddy plant hopper Bt paddy rice and its control non-transgenic paddy rice on include development duration, the life-span with
And the parameter of fecundity；
S2：Calculate age-age survival rate of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S3：Calculate fecundity and population dynamic parameter of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S4：Calculate life-span expectation and reproduction value of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S5：Analyze stable age-age distribution and population of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice
Prediction.
The Bt paddy rice selection turns cry1Ab gene pest-resistant paddy rice mf-MH3301-1, and the control non-transgenic paddy rice is
Compare parent non-transgenic paddy rice MH3301.
It is that brown paddy plant hopper is calculated by Bootstrap methods in Bt paddy rice and its control non-transgenic water in the step S1
Include the parameter of development duration, life-span and fecundity on rice.
The S2-S5 acceptable ages-age both sexes life-table method, it is specific to pass through age-age both sexes life meter
Calculation machine program Twosex-MS Chart carry out analysis age of acquisition-age survival rate, fecundity, kind to step S1 recording parameterses
Group's dynamic parameter, life-span expectation, reproduction value, stable age-age distribution and Population forecast.
Population dynamic parameter calculation formula of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice is as follows：
λ=er；GRR=Σ mx
Wherein, n is the full age number of brown paddy plant hopper, and x and i are the age, and j is age；lxRefer to whole population survival to the general of age x
Rate；SxjRefer to new hatchlings survival to the probability of x ages at age j；mxRefer to eggs on average amount of the population in age x；fxjRefer to it is female into
Eggs on average amount of the worm in age x；R0For net breeding rate；R is intrinsic rate of increase；λ is finite rate of increase；T is Generation means week
Phase；GRR is total reproductive capacity GRR；RxFor age characteristics proliferation rate.
Brown paddy plant hopper single head in teat glass is raised, teat glass diameter 3cm height 20cm.
It is below the specific embodiment of the present invention.
The present invention is to turn cry1Ab gene pest-resistant paddy rice mf-MH3301-1 and control parent's non-transgenic water for examination paddy rice
Rice MH3301, rice varieties are provided by the agriculture genetic engineering key lab of Fujian Academy.
Brown paddy plant hopper picks up from plant virus research institute of University Of Agriculture and Forestry In Fujian, after in Fujian Institute Of Applied Ecological Of Agriculture and Forestry University's gas
Wait case (1 DEG C of 28 scholar of temperature, RH 80% scholar l%, illumination L:D=16:8) in respectively with turn cry1Ab gene pest-resistants paddy rice and its
Non-transgenic paddy rice is compareed continuously to raise for many years so far.
Two kinds of 45 age in days, 10 plants of rice seedlings are taken to be respectively put into water planting in 10 big teat glass respectively, each teat glass connects
Enter 1 bosom female brown paddy plant hopper of ovum, sealed with the gauze of 100 mesh, in growth cabinet (1 DEG C of 28 scholar of temperature, the scholar l% of RH 80%, light
According to L：D=16/8) after spawning 24h, brown paddy plant hopper is removed.The daily hatching situation for regularly observing and recording ovum.Choose just hatching
Nymph raised respectively with corresponding transgenosis and non-transgenic rice seedlings single tube (diameter 3cm height 20cm), each handle 69
Head brown paddy plant hopper, is changed once for examination rice seedlings every 10d, and water planting liquid is changed 1 time every 2d, and daily time recording brown paddy plant hopper sloughs off
Situations such as skin, emergence, death.Deng the brown paddy plant hopper adult pairing taken in brown paddy plant hopper emergence 12h on correspondence rice varieties, record respectively
Its daily egg laying amount and the rice seedlings for changing spawning, until female adult is dead.Whole experiment knot after brown paddy plant hopper adult is all dead
Beam.
The initial data such as all individual development durations of brown paddy plant hopper, survival condition, female adult day egg laying amount pass through age-age
Both sexes life table computer program Twosex-MSChart (Chi, 2015) is analyzed, so as to obtain many life tables ginsengs
Number.Such as：Age-age feature survival rate Sxj, age characteristics survival rate lx, age characteristics fecundity mx, fxjAge-age feature
Fecundity, age-age characteristics life expects exj, the age-age feature reproduction value vxjIt is (x=, j=age), intrinsic to increase
Long rate r, finite rate of increase λ, net breeding rate R0, Generation means cycle T, total reproductive capacity GRR, preoviposition period APOP, before total spawning
Phase TPOP.
Wherein, lxRefer to whole population (including female, male brown paddy plant hopper and all individuals for not being developed to adult death) survival to arrive
Age x probability, sxjRefer to new hatchlings survival to the probability of x ages at age j.fxjRefer to eggs on average of the female adult pest in age x
Amount, mxRefer to eggs on average amount of the population in age x.exjRefer to population at individual to the life expectancy of x ages at age j, vxjRefer to population
Body is in x ages at age j to follow-on average contribution value.Preoviposition period refers to sprouts wings to the time of first time spawning from brown paddy plant hopper,
Total preoviposition period refers to the time for starting spawning from egg development to adult.
Population dynamic parameter calculation formula is as follows： λ=er；GRR=Σ mx；
Wherein, n is the full age number of brown paddy plant hopper, and x and i are the age, and j is age；lxRefer to whole population survival to the general of age x
Rate；SxjRefer to new hatchlings survival to the probability of x ages at age j；mxRefer to eggs on average amount of the population in age x；fxjRefer to it is female into
Eggs on average amount of the worm in age x；R0For net breeding rate；R is intrinsic rate of increase；λ is finite rate of increase；T is Generation means week
Phase；GRR is total reproductive capacity GRR；RxFor age characteristics proliferation rate.
(1) parameter such as development duration, life-span and the fecundity of brown paddy plant hopper on Bt paddy rice and its control
As shown in Table 1, in addition to an age and five ages, the development time of brown paddy plant hopper each age in Bt paddy rice mf-MH3301-1 and
Non-transgenic reference MH3301 is not significantly different (P ＞ 0.05).The wherein age of brown paddy plant hopper one is in Bt paddy rice mf-MH3301-1 and non-
Non-transgenic control MH3301 development time is respectively 2.43 days and 2.77 days, and difference reaches the pole level of signifiance (P ＜ 0.01).It is brown
The age of plant hopper five is respectively 3.82 days and 4.20 in Bt paddy rice mf-MH3301-1 and non-transgenic reference MH3301 development time
My god, reach significant difference (P ＜ 0.05).Brown paddy plant hopper is female on Bt paddy rice mf-MH3301-1 and non-transgenic reference MH3301
Worm life-span, longevity of male and overall life are not significantly different (P ＞ 0.05).Female brown paddy plant hopper is in Bt paddy rice mf-MH3301-1
On fecundity be noticeably greater than non-transgenic reference MH3301 (P ＜ 0.01)；And preoviposition period and total preoviposition period do not show
Write difference (P ＞ 0.05).
Table 1
Data are average value ± standard errors in table, and with after data line, different letters are represented through paired respectively
Bootstrap test examine significant difference.
(2) age-age survival rate of the brown paddy plant hopper on Bt paddy rice and non-transgenic reference
Age-age survival rate sxjRefer to new hatchlings survival to the probability of x ages at age j.Due between insect individual
And between male and female developmental rate difference, therefore there is obvious overlapping phenomenon.Age-age Survival curves (sxj) not only
The survival condition that different larval instar brown paddy plant hopper changes over time on two kinds of paddy rice is described, and age differentiation and age can be reflected
Phase overlapping phenomenon, so age-age Survival curves (sxj) more accurately describe the Population of Rice Brown Planthopper for taking food two kinds of paddy rice
Development and the situation of change of survival rate.Before from ovum phase to adult, Survival curves of the brown paddy plant hopper on two kinds of paddy rice are basic
It is similar.But in adult stage, the survival rate for taking food Bt paddy rice mf-MH3301-1 brown paddy plant hopper male worm is significantly lower than female adult；Take
The survival rate of the food non-transgenic reference MH3301 female male worm of brown paddy plant hopper has no significant difference.When the 5th age nymph survives in 26d
When rate is reduced to zero, the survival rate of brown paddy plant hopper adult reaches that (Fig. 2, wherein A Bt paddy rice mf-MH-3301-1, B is non-turns base to maximum
Because of paddy rice MH3301).
(3) fecundity and population dynamic parameter of the brown paddy plant hopper on transgenic pest-resistant rice and its control
Age characteristics survival rate lxRefer to whole population and (including female, male brown paddy plant hopper and be not developed to dead all of adult
Body) survive to age x probability.Compared with taking food non-transgenic paddy rice MH3301, Bt paddy rice mf- is taken food with age
MH3301-1 brown paddy plant hopper age characteristics survival rate (lx) substantially comparison is slow for decrease speed.Age-age feature fecundity fxj
Refer to female adult pest in age x eggs on average amount, age characteristics fecundity mxRefer to eggs on average amount of the population in age x.Take food Bt
The paddy rice mf-MH3301-1 brown paddy plant hopper age-age feature fecundity (fxj) and population age feature fecundity (mx) significantly high
In control MH3301, therefore take food the population age feature relative reproductive value (l of Bt paddy rice mf-MH3301-1 brown paddy plant hopperxmx) also notable
Higher than control MH3301.When brown paddy plant hopper takes food on Bt paddy rice, 43d reaches maximum fecundity 13.5；But turn base non-
During because being taken food on paddy rice, 49d just reaches maximum fecundity 10, delayed 6d (Fig. 3, wherein A Bt paddy rice mf-MH-3301-1,
B non-transgenics paddy rice MH3301).
Due to gross fecundity GRR=∑s mx, net breeding rateTherefore mf-MH3301-1 brown paddy plant hopper is taken food
Gross fecundity and net breeding rate be significantly higher than control MH3301, be its (P ＜ 0.05) more than 2 times.Similarly, mf-MH3301- is taken food
The intrinsic rate of increase r and finite rate of increase λ of 1 brown paddy plant hopper are also significantly greater than control MH3301 (P ＜ 0.05).And brown paddy plant hopper is two
The Generation means cycle T planted on paddy rice has no significant difference (P ＞ 0.05) (table 2).
Table 2
Note：Data are average value ± standard errors in table, and with after data line, different letters are represented through paired respectively
Bootstrap test examine significant difference.
(4) life-span expectation and reproduction value of the brown paddy plant hopper on two kinds of paddy rice
Age-age life-span expectation exjRefer to population at individual to the life expectancy of x ages at age j.In view of sex and individual
The difference of developmental rate between body, utilizes life-span expectation curve of the brown paddy plant hopper on two kinds of paddy rice, it can be seen that different sexes with
And the change of different larval instar life-span expectation.Life-span expectation (the e of ovum phase initial zero0,1) be Average Life of Species value, this and table 1
In life-span it is identical.Increase over time, brown paddy plant hopper gradually reduces in the life-span expectation of two kinds of paddy rice.But, in Bt water
The life-span expectation of brown paddy plant hopper female adult is higher than male worm on rice；And on the contrary, the longevity of brown paddy plant hopper male worm on non-transgenic reference
Life is expected to be higher than female adult (Fig. 4, wherein A Bt paddy rice mf-MH-3301-1, B non-transgenic paddy rice MH3301).
Age-age reproduction value vxjRefer to population at individual in x ages at age j to follow-on average contribution value.The ovum phase exists
The corresponding reproduction value (v of zero point0,1) it is finite rate of increase of the Population of Rice Brown Planthopper on two kinds of paddy rice.With the increase of age,
The reproduction to offspring of brown paddy plant hopper individual is worth more and more higher, and maximum is reached in the female adult stage.In Bt paddy rice and non-transgenic
In control, maximum reproduction value is respectively (v37,7=50.89) and (v35,7=36.05).Wherein, brown paddy plant hopper is in the 5th age and female adult pest
Stage, the reproduction value that brown paddy plant hopper is taken food on Bt paddy rice is higher than non-transgenic reference (Fig. 5, wherein A Bt paddy rice mf-MH-
3301-1, B non-transgenic paddy rice MH3301).
(5) stable age-age distribution and Population forecast of the brown paddy plant hopper on two kinds of paddy rice
As shown in Figure 6 (wherein A Bt paddy rice mf-MH-3301-1, B non-transgenic paddy rice MH3301), according to age-age
Both sexes life table data try to achieve respectively under ideal conditions (food, space are unrestricted, no natural enemy interference) brown paddy plant hopper in Bt water
Stable age-age distribution on rice mf-MH3301-1 and non-transgenic paddy rice MH3301.Wherein, brown paddy plant hopper is on Bt paddy rice
Final stable age distribution be：Ovum phase=60.42%, adult stage=5.58% of nymph=34.00%.Brown paddy plant hopper is at non-turn
Final stable age distribution on trans-genetic hybrid rice is：Ovum phase=52.26%, nymph=39.48%, adult stage=8.26%.
As shown in Figure 7 (wherein A Bt paddy rice mf-MH-3301-1, B non-transgenic paddy rice MH3301), brown paddy plant hopper is in Bt water
Population increase rate prediction on rice mf-MH3301-1 and non-transgenic paddy rice MH3301.Pass through age-age both sexes life table
The Population forecast of structure is easy to be depicted the variation tendency of age structure.Because age-age both sexes life table can be described
Age breaks up, and brown paddy plant hopper each age can show.
Above is presently preferred embodiments of the present invention, all changes made according to technical solution of the present invention, produced function is made
During with scope without departing from technical solution of the present invention, protection scope of the present invention is belonged to.
Claims (5)
1. a kind of Bt paddy rice is to the method for evaluating safety of non-target insects brown paddy plant hopper, it is characterised in that：Comprise the following steps,
S1：Calculating and recording brown paddy plant hopper includes development duration, life-span and life on Bt paddy rice and its control non-transgenic paddy rice
Grow the parameter of power；
S2：Calculate age-age survival rate of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S3：Calculate fecundity and population dynamic parameter of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S4：Calculate life-span expectation and reproduction value of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
S5：Analyze stable age-age distribution and Population forecast of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice；
Population dynamic parameter calculation formula of the brown paddy plant hopper on Bt paddy rice and its control non-transgenic paddy rice is as follows：
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</mrow>
<mrow>
<mi>&lambda;</mi>
<mo>=</mo>
<msup>
<mi>e</mi>
<mi>r</mi>
</msup>
<mo>;</mo>
<mi>T</mi>
<mo>=</mo>
<mfrac>
<mrow>
<msub>
<mi>lnR</mi>
<mn>0</mn>
</msub>
</mrow>
<mi>r</mi>
</mfrac>
<mo>;</mo>
<mi>G</mi>
<mi>R</mi>
<mi>R</mi>
<mo>=</mo>
<mo>&Sigma;</mo>
<msub>
<mi>m</mi>
<mi>x</mi>
</msub>
<mo>;</mo>
<mi>R</mi>
<mi>x</mi>
<mo>=</mo>
<munderover>
<mo>&Sigma;</mo>
<mrow>
<mi>i</mi>
<mo>=</mo>
<mn>0</mn>
</mrow>
<mi>x</mi>
</munderover>
<msub>
<mi>l</mi>
<mi>i</mi>
</msub>
<msub>
<mi>m</mi>
<mi>i</mi>
</msub>
</mrow>
Wherein, n is the full age number of brown paddy plant hopper, and x and i are the age, and j is age；lxRefer to whole population survival to age x probability；
SxjRefer to new hatchlings survival to the probability of x ages at age j；mxRefer to eggs on average amount of the population in age x；fxjRefer to female adult pest to exist
Age x eggs on average amount；R0For net breeding rate；R is intrinsic rate of increase；λ is finite rate of increase；T is the Generation means cycle；
GRR is total reproductive capacity GRR；RxFor age characteristics proliferation rate.
2. Bt paddy rice according to claim 1 is to the method for evaluating safety of non-target insects brown paddy plant hopper, it is characterised in that：
The Bt paddy rice selection turns cry1Ab gene pest-resistant paddy rice mf-MH3301-1, and the control non-transgenic paddy rice is control parent
Non-transgenic paddy rice MH3301.
3. Bt paddy rice according to claim 1 is to the method for evaluating safety of non-target insects brown paddy plant hopper, it is characterised in that：
It is to calculate brown paddy plant hopper by Bootstrap methods on Bt paddy rice and its control non-transgenic paddy rice to include in the step S1
The parameter of development duration, life-span and fecundity.
4. Bt paddy rice according to claim 1 is to the method for evaluating safety of non-target insects brown paddy plant hopper, it is characterised in that：
The S2-S5 acceptable ages-age both sexes life-table method, it is specific to pass through age-age both sexes life table computer program
Twosex-MS Chart carry out analysis age of acquisition-age survival rate, fecundity, population dynamic to step S1 recording parameterses to join
Number, life-span expectation, reproduction value, stable age-age distribution and Population forecast.
5. Bt paddy rice according to claim 1 is to the method for evaluating safety of non-target insects brown paddy plant hopper, it is characterised in that：
Brown paddy plant hopper single head in teat glass is raised, teat glass diameter 3cm height 20cm.
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