CN109111530A - 一种具有抗氧化活性的复合多糖及其制备方法和应用 - Google Patents
一种具有抗氧化活性的复合多糖及其制备方法和应用 Download PDFInfo
- Publication number
- CN109111530A CN109111530A CN201810737396.6A CN201810737396A CN109111530A CN 109111530 A CN109111530 A CN 109111530A CN 201810737396 A CN201810737396 A CN 201810737396A CN 109111530 A CN109111530 A CN 109111530A
- Authority
- CN
- China
- Prior art keywords
- complex polysaccharide
- preparation
- antioxidant activity
- parts
- portions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000004676 glycans Chemical class 0.000 title claims abstract description 47
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 47
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 47
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 12
- 239000003814 drug Substances 0.000 claims abstract description 11
- 244000037364 Cinnamomum aromaticum Species 0.000 claims abstract description 9
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims abstract description 9
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims abstract description 9
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims abstract description 9
- 235000009685 Crataegus X maligna Nutrition 0.000 claims abstract description 9
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims abstract description 9
- 235000009486 Crataegus bullatus Nutrition 0.000 claims abstract description 9
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims abstract description 9
- 235000009682 Crataegus limnophila Nutrition 0.000 claims abstract description 9
- 235000004423 Crataegus monogyna Nutrition 0.000 claims abstract description 9
- 235000002313 Crataegus paludosa Nutrition 0.000 claims abstract description 9
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims abstract description 9
- 244000130270 Fagopyrum tataricum Species 0.000 claims abstract description 9
- 235000014693 Fagopyrum tataricum Nutrition 0.000 claims abstract description 9
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 9
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 9
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 9
- 229940079593 drug Drugs 0.000 claims abstract description 9
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims abstract description 8
- 244000197580 Poria cocos Species 0.000 claims abstract description 8
- 235000008599 Poria cocos Nutrition 0.000 claims abstract description 8
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims abstract description 8
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims abstract description 8
- 235000013402 health food Nutrition 0.000 claims abstract description 8
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims abstract description 7
- -1 galactolipin Chemical compound 0.000 claims abstract description 6
- 238000000502 dialysis Methods 0.000 claims abstract description 5
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 4
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 4
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 239000008103 glucose Substances 0.000 claims abstract description 4
- 229940097043 glucuronic acid Drugs 0.000 claims abstract description 4
- 238000001556 precipitation Methods 0.000 claims abstract description 4
- 238000005119 centrifugation Methods 0.000 claims abstract description 3
- 240000000171 Crataegus monogyna Species 0.000 claims abstract 3
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-galactopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 claims abstract 2
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 claims abstract 2
- 230000003064 anti-oxidating effect Effects 0.000 claims description 5
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000002243 precursor Substances 0.000 claims description 5
- 239000008399 tap water Substances 0.000 claims description 5
- 235000020679 tap water Nutrition 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 3
- 230000008719 thickening Effects 0.000 claims description 2
- 241001251949 Xanthium sibiricum Species 0.000 claims 1
- 239000003963 antioxidant agent Substances 0.000 abstract description 3
- 235000006708 antioxidants Nutrition 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 8
- 150000003254 radicals Chemical class 0.000 description 7
- 241001092040 Crataegus Species 0.000 description 6
- 230000007760 free radical scavenging Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 4
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000002775 capsule Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 229940100688 oral solution Drugs 0.000 description 3
- 239000006187 pill Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000003826 tablet Substances 0.000 description 3
- 238000001157 Fourier transform infrared spectrum Methods 0.000 description 2
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000003859 lipid peroxidation Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- UOQHWNPVNXSDDO-UHFFFAOYSA-N 3-bromoimidazo[1,2-a]pyridine-6-carbonitrile Chemical compound C1=CC(C#N)=CN2C(Br)=CN=C21 UOQHWNPVNXSDDO-UHFFFAOYSA-N 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 230000006369 cell cycle progression Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000004110 gluconeogenesis Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229940099563 lactobionic acid Drugs 0.000 description 1
- 150000002632 lipids Chemical group 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003617 peroxidasic effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0087—Glucomannans or galactomannans; Tara or tara gum, i.e. D-mannose and D-galactose units, e.g. from Cesalpinia spinosa; Tamarind gum, i.e. D-galactose, D-glucose and D-xylose units, e.g. from Tamarindus indica; Gum Arabic, i.e. L-arabinose, L-rhamnose, D-galactose and D-glucuronic acid units, e.g. from Acacia Senegal or Acacia Seyal; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Food Science & Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nutrition Science (AREA)
- Epidemiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Sustainable Development (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Emergency Medicine (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
本发明公开了一种具有抗氧化活性的复合多糖及其制备方法和应用。所述复合多糖是由葡萄糖、甘露糖、阿拉伯糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸组成，摩尔质量比为50.6～51.8:20.4～20.9:2.9～3.1:2.6～2.8:1.7～2.0:17.2～17.8:1.8～4.2。制备方法是将40～120份山楂、20～60份荷叶、20～60份苦荞麦、15～45份决明子、10～30份枸杞、10～30份茯苓分别烘干粉碎，再混合均匀后，依次经过水提、抽滤、浓缩、醇沉、离心、透析、冷冻干燥，得到复合多糖。本发明制备的复合多糖纯度高、抗氧化活性强，提取工艺简便、成本低廉、可操作性强，可广泛应用于药物、保健食品等行业中，同时也可作为中间体广泛运用于抗氧化各个领域中。
Description
技术领域
本发明属于药物及保健品领域，具体涉及一种复合多糖及其制备方法和应用，尤其是一种具有抗氧化活性的复合多糖及其制备方法和应用。
背景技术
当人体在与外界环境接触时，阳光辐射、空气污染、农药、药物、放射、吸烟、压力等因素都会使人体产生自由基，过量自由基通过多种途径诱导人体组织、器官的细胞衰老和凋亡，引起DNA损伤，抑制细胞周期进程；通过影响体内酶代谢，加速脂肪和蛋白质的分解，促进糖异生过程，削弱糖氧化功能作用；降低机体抵抗力和免疫力，引发衰老、肿瘤、高血压、心脏病、糖尿病等疾病。
科学研究表明，多糖具有清除自由基的抗氧化作用，通过捕捉脂质过氧化链式反应中产生的自由基，从而减少脂质过氧化反应链长度、阻断或减缓脂质过氧化的进行，达到对自由基的直接清除作用；通过与产生自由基所必需的金属离子发生络合作用，对自由基起间接清除作用；多糖可以提高SOD、CAT、GSH-Px等抗氧化酶的活性，从而发挥抗氧化作用。
发明内容
本发明的目的在于提供一种纯度高、提取工艺简便、抗氧化活性强的复合多糖。
本发明的另一目的在于提供上述具有抗氧化活性的复合多糖的制备方法。
本发明的再一目的在于提供上述具有抗氧化活性的复合多糖的实际应用。
为实现本发明的目的，本发明采用如下技术方案：
一种具有抗氧化活性的复合多糖，
由葡萄糖、甘露糖、阿拉伯糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸按照摩尔质量比50.6～51.8:20.4～20.9:2.9～3.1:2.6～2.8:1.7～2.0:17.2～17.8:1.8～4.2组成。
一种具有抗氧化活性的复合多糖的制备方法，将40～120份山楂、20～60份荷叶、20～60份苦荞麦、15～45份决明子、10～30份枸杞、10～30份茯苓分别烘干粉碎，再混合均匀后，依次经过水提、抽滤、浓缩、醇沉、离心、透析、冷冻干燥，得到复合多糖。
而且，山楂、荷叶、苦荞麦、决明子、枸杞、茯苓的重量比为4：2：2：1.5：1：1。
而且，所述水提是加水体积是原料量的5～40倍v/m，水温40～80℃，提取次数1～4，提取时间15～120min。
而且，所述浓缩为浓缩温度50～60℃，浓缩至浓缩前体积的1/5。
而且，所述醇沉是指加入无水乙醇至乙醇浓度为75～80％v/v进行沉淀。
而且，所述透析为用Mw3500的透析袋自来水透析2天，蒸馏水透析1天。
本发明中所述山楂、荷叶、苦荞麦、决明子、枸杞、茯苓是本领域技术人员公知的，可以通过商业途径购买得到，可以为药材，也可以是饮片，只要其符合《中华人民共和国药典》(2015年版)质量标准即可。
本发明所述复合多糖的使用剂量和使用方法取决于诸多因素，包括使用者的年龄、体重、性别、自然健康状况、营养状况、使用时间、代谢速率、病程严重程度以及主治医师的主观判断。本领域的技术人员依据上述因素可以确定所述复合多糖的使用剂量和使用方法。
本领域技术人员可以根据不同使用者的需求将本发明所述复合多糖加入制备不同剂型时所需的各种常规的添加剂，如崩解剂、润滑剂、乳化剂、粘合剂等，以常规制剂方法，制成常用药物或保健食品，如胶囊剂、片剂、丸剂、口服液、粉剂、颗粒剂、散剂等。
本发明提供了一种具有抗氧化作用的保健食品，包括有效量的本发明所述复合多糖和保健食品中可接受的辅料。
所述保健食品优选为胶囊剂、片剂、丸剂、粉剂、颗粒剂、散剂或口服液。
本发明还提供了一种具有抗氧化作用的药物，包括有效量的本发明所述复合多糖和药学上可接受的辅料。
所述药物优选为胶囊剂、片剂、丸剂、粉剂、颗粒剂、散剂或口服液。
所述一种具有抗氧化活性的复合多糖在抗氧化活性领域的应用，特别用于抗氧化活性保健食品和药物领域。
本发明的优点和有益效果：
1、本发明制备的复合多糖纯度高、抗氧化活性强，对DPPH、ABTS、OH自由基清除率分别达到93.56％、83.29％、84.03％。
2、本发明提取工艺简便、成本低廉、可操作性强，可广泛应用于药物、保健食品等行业中，同时也可作为中间体广泛运用于抗氧化各个领域中。
附图说明
图1为本发明所述复合多糖的傅里叶红外光谱图；
图2为复合多糖对DPPH自由基清除率效果图；
图3为复合多糖对ABTS自由基清除率效果图；
图4为复合多糖对OH自由基清除率效果图。
具体实施方式
本发明公开了一种具有抗氧化活性的复合多糖及其制备方法和应用。本领域技术人员可以借鉴本文内容，适当改进工艺参数实现。特别需要指出的是，所有类似的替换和改动对本领域技术人员来说是显而易见的，它们都被视为包括在本发明。本发明的方法及产品已经通过较佳实施例进行了描述，相关人员明显能在不脱离本发明内容、精神和范围内对本文所述的方法进行改动或适当变更与组合，来实现和应用本发明技术。
为了进一步理解本发明，下面将结合本发明实施例，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
实施例1
一种具有抗氧化活性的复合多糖，由葡萄糖、甘露糖、阿拉伯糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸按照摩尔质量比50.6:20.4:3.1:2.6:1.7组成。
制备方法为：将山楂40份、荷叶20份、苦荞麦20份、决明子15份、枸杞10份、茯苓10份分别于40～60℃烘干至恒重、粉碎。然后进行混合，加入2875mL水量，提取温度55℃，提取时间45min，提取次数3，接着抽滤，收集滤液加入0.2％果胶酶于50℃处理2h，随后于95℃灭活5min，经55℃浓缩至浓缩前体积的1/5，其后加入无水乙醇至乙醇浓度为80％(v/v)进行沉淀，于4500r/min离心15min，收集上清液并用Mw3500的透析袋自来水透析2天，蒸馏水透析1天，将透析液于-20℃冷冻干燥，最后得到复合多糖，多糖得率为5.47％。
实施例2
将山楂40份、荷叶20份、苦荞麦20份、决明子15份、枸杞10份、茯苓10份分别于40～60℃烘干至恒重、粉碎。然后进行混合，然后加入3450mL水量，提取温度60℃，提取时间75min，提取次数3，接着抽滤，收集滤液加入0.2％果胶酶于50℃处理2h，随后于95℃灭活5min，经55℃浓缩至浓缩前体积的1/5，其后加入无水乙醇至乙醇浓度为80％(v/v)进行沉淀，于4500r/min离心15min，收集上清液并用Mw3500的透析袋自来水透析2天，蒸馏水透析1天，将透析液于-20℃冷冻干燥，最后得到复合多糖，多糖得率为5.85％。
实施例3
将山楂40份、荷叶20份、苦荞麦20份、决明子15份、枸杞10份、茯苓10份分别于40～60℃烘干至恒重、粉碎。然后加入4025mL水量，提取温度65℃，提取时间60min，提取次数3，接着抽滤，收集滤液加入0.2％果胶酶于50℃处理2h，随后于95℃灭活5min，经55℃浓缩至浓缩前体积的1/5，其后加入无水乙醇至乙醇浓度为80％(v/v)进行沉淀，于4500r/min离心15min，收集上清液并用Mw3500的透析袋自来水透析2天，蒸馏水透析1天，将透析液于-20℃冷冻干燥，最后得到复合多糖，多糖得率为6.35％。
对本发明的复合多糖进行傅里叶红外光谱分析，如图1所示，所述复合多糖具备以下主要官能团：O-H，3388.93cm-1；C-H，2975.27，2928.88，2898.95cm-1；C＝O，1716.34，1609.28，1416.72cm-1；C-O-C，1146.65，1088.71cm-1；C-O-H，1055.02cm-1。
对本发明的复合多糖对DPPH、ABTS、OH自由基清除率进行分析，如图2～4所示，结果表明，本发明制备的复合多糖对DPPH、ABTS、OH自由基清除率分别达到93.56％、83.29％、84.03％，具有很强的抗氧化活性。
Claims (8)
1.一种具有抗氧化活性的复合多糖，其特征在于：
由葡萄糖、甘露糖、阿拉伯糖、半乳糖、木糖、半乳糖醛酸、葡萄糖醛酸按照摩尔质量比50.6～51.8:20.4～20.9:2.9～3.1:2.6～2.8:1.7～2.0:17.2～17.8:1.8～4.2组成。
2.根据权利要求1所述的具有抗氧化活性的复合多糖的制备方法，其特征在于：将40～120份山楂、20～60份荷叶、20～60份苦荞麦、15～45份决明子、10～30份枸杞、10～30份茯苓分别烘干粉碎，再混合均匀后，依次经过水提、抽滤、浓缩、醇沉、离心、透析、冷冻干燥，得到复合多糖。
3.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法，其特征在于：山楂、荷叶、苦荞麦、决明子、枸杞、茯苓的重量比为4：2：2：1.5：1：1。
4.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法，其特征在于：所述水提是加水体积是原料量的5～40倍v/m，水温40～80℃，提取次数1～4，提取时间15～120min。
5.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法，其特征在于：所述浓缩为浓缩温度50～60℃，浓缩至浓缩前体积的1/5。
6.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法，其特征在于：所述醇沉是指加入无水乙醇至乙醇浓度为75～80％v/v进行沉淀。
7.根据权利要求2所述的具有抗氧化活性的复合多糖的制备方法，其特征在于：所述透析为用Mw3500的透析袋自来水透析2天，蒸馏水透析1天。
8.一种权利要求1～7任一权利要求所述的复合多糖在制备具有抗氧化作用的药物或保健食品中的应用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810737396.6A CN109111530A (zh) | 2018-07-06 | 2018-07-06 | 一种具有抗氧化活性的复合多糖及其制备方法和应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810737396.6A CN109111530A (zh) | 2018-07-06 | 2018-07-06 | 一种具有抗氧化活性的复合多糖及其制备方法和应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109111530A true CN109111530A (zh) | 2019-01-01 |
Family
ID=64822912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810737396.6A Pending CN109111530A (zh) | 2018-07-06 | 2018-07-06 | 一种具有抗氧化活性的复合多糖及其制备方法和应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109111530A (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112425779A (zh) * | 2020-11-02 | 2021-03-02 | 安徽金源药业有限公司 | 一种茯苓山药多糖颗粒物及其应用 |
| CN113061195A (zh) * | 2021-04-01 | 2021-07-02 | 天津市食品安全检测技术研究院 | 一种具有降血脂作用的复合多糖及其制备方法和应用 |
| CN113100368A (zh) * | 2021-04-29 | 2021-07-13 | 安徽大学 | 一种抗氧化荷叶饮料及其制备方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102627789A (zh) * | 2012-03-31 | 2012-08-08 | 中华全国供销合作总社济南果品研究院 | 一种食用菌复合多糖的制备工艺 |
| CN103622989A (zh) * | 2013-11-21 | 2014-03-12 | 无限极（中国）有限公司 | 一种复合多糖组合物及其制备方法和应用 |
| CN104530251A (zh) * | 2014-12-22 | 2015-04-22 | 中国科学院西北高原生物研究所 | 一种抗氧化复合多糖及其制备方法 |
| CN105362285A (zh) * | 2015-10-22 | 2016-03-02 | 南昌大学 | 一种具有促进胃排空作用的复合多糖的制备方法及复合多糖的应用 |
-
2018
- 2018-07-06 CN CN201810737396.6A patent/CN109111530A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102627789A (zh) * | 2012-03-31 | 2012-08-08 | 中华全国供销合作总社济南果品研究院 | 一种食用菌复合多糖的制备工艺 |
| CN103622989A (zh) * | 2013-11-21 | 2014-03-12 | 无限极（中国）有限公司 | 一种复合多糖组合物及其制备方法和应用 |
| CN104530251A (zh) * | 2014-12-22 | 2015-04-22 | 中国科学院西北高原生物研究所 | 一种抗氧化复合多糖及其制备方法 |
| CN105362285A (zh) * | 2015-10-22 | 2016-03-02 | 南昌大学 | 一种具有促进胃排空作用的复合多糖的制备方法及复合多糖的应用 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112425779A (zh) * | 2020-11-02 | 2021-03-02 | 安徽金源药业有限公司 | 一种茯苓山药多糖颗粒物及其应用 |
| CN113061195A (zh) * | 2021-04-01 | 2021-07-02 | 天津市食品安全检测技术研究院 | 一种具有降血脂作用的复合多糖及其制备方法和应用 |
| CN113100368A (zh) * | 2021-04-29 | 2021-07-13 | 安徽大学 | 一种抗氧化荷叶饮料及其制备方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109111530A (zh) | 一种具有抗氧化活性的复合多糖及其制备方法和应用 | |
| CN107822948A (zh) | 具有晒后修护、抗氧化、美白功效的组合物及其制备方法 | |
| CN101822373B (zh) | 金耳银杏功能性食品、制备方法及用途 | |
| CN109181841A (zh) | 一种高油酸花生的榨油工艺及其应用 | |
| CN110343185B (zh) | 一种酒制黄精多糖及其在脾虚免疫功能调节方面的用途 | |
| CN101129790A (zh) | 复方沙棘含片及其制备方法 | |
| CN103992876A (zh) | 一种西番莲籽油的提取方法 | |
| CN104905237A (zh) | 桦褐孔菌复合冲剂及其生产方法 | |
| Chen et al. | Review of isolation, purification, structural characteristics and bioactivities of polysaccharides from Portulaca oleracea L | |
| CN107296772A (zh) | 一种含有圣罗勒提取物的护肤品及其制备 | |
| CN104667135A (zh) | 一种药物组合物、保健品及制备方法 | |
| CN104799286A (zh) | 一种用于提高免疫力的组合物及其制备方法 | |
| KR101351187B1 (ko) | 미역귀를 이용한 항산화 기능성 화장품 원료추출방법 및 이를 이용한 항산화기능성 화장품 | |
| CN103815397B (zh) | 调降血压、血脂和血糖的食品组合物及其制备方法 | |
| CN105192820A (zh) | 一种降血脂蛹虫草多糖饮料及其制备方法 | |
| CN104004110A (zh) | 一种从瓜子金中提取的瓜子金多糖及其应用 | |
| Yu et al. | Recent advances in polysaccharides from Chaenomeles speciosa (sweet) Nakai.: extraction, purification, structural characteristics, health benefits, and applications | |
| KR102421800B1 (ko) | 고염 미생물의 발효 추출물을 포함하는 조성물 | |
| CN107519213A (zh) | 一种含有猴头菇提取物的护胃强免疫组合物及其制备方法 | |
| CN104435072B (zh) | 一种具有辅助降血糖、降血脂的提取物及其制备方法 | |
| CN103110552B (zh) | 一种黄花柳牙膏的制备方法 | |
| CN113501858A (zh) | 电磁裂解技术提取荞麦小分子肽液和肽粉的方法 | |
| CN105998528A (zh) | 一种口腔保健刷牙粉 | |
| CN106668332B (zh) | 一种百香果含片及其制备方法 | |
| CN108743727A (zh) | 一种具有抗氧化活性的复合黄酮及其制备方法和应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190101 |