CN109106792B - Traditional Chinese medicine compound preparation for inhibiting blue ear virus and preparation method thereof - Google Patents
Traditional Chinese medicine compound preparation for inhibiting blue ear virus and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of traditional Chinese medicine preparations, and particularly discloses a traditional Chinese medicine compound preparation for inhibiting a blue ear virus and a preparation method thereof. The weight portions of the components are as follows: 10-20 parts of astragalus membranaceus, 5-10 parts of rheum officinale, 10-20 parts of folium isatidis, 5-15 parts of fructus forsythiae, 5-15 parts of fructus gardeniae, 5-15 parts of sweet wormwood, 5-15 parts of honeysuckle, 5-15 parts of radix bupleuri, 5-15 parts of herba houttuyniae and 10-20 parts of folium mori, wherein the sum of the parts by weight of the raw materials is 100 parts. The invention can regulate yin and yang, treat both manifestation and root cause of disease, make organism be same in physique and immunity, and balance yin and yang, thereby achieving an ideal breeding state of 'having few diseases and being died', and effectively inhibiting the blue ear virus.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine compound preparation for inhibiting a blue ear virus and a preparation method thereof.
Background
The porcine reproductive and respiratory syndrome is a second type infectious disease in China, has high contagious property and has the local epidemic characteristic. The porcine reproductive and respiratory syndrome virus only infects pigs, other animals do not infect the pigs, and pigs of various ages and breeds of days can be infected with the virus, wherein piglets and pregnant sows with the ages of days within 1 month are the most susceptible pig groups.
The porcine reproductive and respiratory syndrome is mainly characterized by dysgenesis, dyspnea, blue purple of ears and other infectious diseases. The pigs can be infected at different ages and varieties, the incidence rate is high, the incidence rate accounts for 50-80% of the whole pig group, the sick pigs die quickly, and the death rate is as high as 80%. Sepsis is mostly a change after death. Pregnant sows and piglets are most susceptible to infection, and late-stage abortion, stillbirth and mummy of pregnant sows occur.
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is a reproductive and respiratory syndrome virus, the primary target cells of PRRSV are the monocyte-macrophage system such as alveolar macrophages, alveolar type ii pneumocytes, peripheral monocytes, epithelial germ cells of the seminiferous tubules and macrophages in the stroma, macrophages in ovarian follicles. Of these, porcine alveolar macrophages are the most sensitive.
The sick sows are mainly characterized by depression, reduced or abolished appetite, fever, dyspnea of different degrees, and abortion, premature birth, stillbirth, mummy and weak piglets of the sows at the later stage of pregnancy (105-107 days). The sow abortion rate can reach 50-70%, the mortality rate can reach more than 35%, the mummy can reach 25%, part of newborn piglets show symptoms such as dyspnea, dyskinesia and paresis, and the mortality rate is obviously increased (40-80%) within 1 week after delivery. A few sows show postpartum agalactia, retained fetal membranes and increased vaginal secretions.
Piglets of 1 month of age show typical respiratory symptoms, dyspnea, sometimes abdominal breathing, loss or abolition of appetite, body temperature rise above 40 ℃, diarrhea. Coarse and disorganized hair, ataxia, progressive wasting, edema of the eyelids. The ear and body surface skin of a small part of piglets can be purple, the death rate of the piglets before weaning can reach 80-100%, the weight gain of the piglets after weaning is reduced, the daily weight gain can be reduced by 50-75%, and the death rate is increased (10-25%). The pig can resist the slow growth and is easy to have other secondary diseases.
Growing and finishing pigs show mild clinical symptoms with different procedures of respiratory symptoms, and few cases can show cough and deep purple color on the back, edge, abdomen and tail skin of ears. Infected pigs are susceptible to secondary infection and the corresponding symptoms.
The disease rate of breeding boars is low and mainly shows general clinical symptoms, but the semen quality of boars is reduced, the semen is malformed, and the semen can carry virus.
The traditional Chinese medicine is safe and effective in treatment, and has no residue and drug resistance. Therefore, if a traditional Chinese medicine compound preparation with good curative effect can be developed, the traditional Chinese medicine compound preparation has obvious economic and social significance for the pig breeding industry.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine compound preparation for inhibiting a blue ear virus and a preparation method thereof.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a traditional Chinese medicine compound preparation for inhibiting a blue ear virus comprises the following components in parts by weight: 10-20 parts of astragalus membranaceus, 5-10 parts of rheum officinale, 10-20 parts of folium isatidis, 5-15 parts of fructus forsythiae, 5-15 parts of fructus gardeniae, 5-15 parts of sweet wormwood, 5-15 parts of honeysuckle, 5-15 parts of radix bupleuri, 5-15 parts of herba houttuyniae and 10-20 parts of folium mori, wherein the sum of the parts by weight of the raw materials is 100 parts.
Preferably, the following three parts by weight are preferred:
10 parts of astragalus membranaceus, 10 parts of rheum officinale, 10 parts of folium isatidis, 15 parts of fructus forsythiae, 5 parts of fructus gardeniae, 15 parts of sweet wormwood, 5 parts of honeysuckle, 10 parts of radix bupleuri, 5 parts of herba houttuyniae and 15 parts of folium mori;
20 parts of astragalus membranaceus, 5 parts of rheum officinale, 15 parts of folium isatidis, 5 parts of fructus forsythiae, 10 parts of fructus gardeniae, 5 parts of sweet wormwood herb, 15 parts of honeysuckle, 5 parts of radix bupleuri, 10 parts of herba houttuyniae and 10 parts of folium mori;
③ 15 parts of astragalus, 8 parts of rhubarb, 10 parts of dyers woad leaf, 10 parts of weeping forsythia, 8 parts of cape jasmine fruit, 9 parts of sweet wormwood, 10 parts of honeysuckle, 10 parts of radix bupleuri, 10 parts of heartleaf houttuynia herb and 10 parts of mulberry leaf.
From the perspective of traditional Chinese veterinary medicine, porcine reproductive and respiratory syndrome is analyzed, and the 'deficiency-evil coordination' is a cause of morbidity, particularly the morbidity and mortality of suckling piglets are serious, namely the five internal organs in the traditional Chinese medicine are in deficiency, and the virus enters in the deficiency to cause exuberance and decline of pathogenic factors and disease resistance; the disease is frequent in summer, and it is usually caused by exogenous dampness-heat transforming into fire, resulting in failure of lung qi to disperse, abnormal ascending and descending, and imbalance of yin and yang. The porcine reproductive and respiratory syndrome belongs to warm diseases, and the treatment is preferably 'heat clearing and detoxifying', 'lung heat clearing and cough relieving', 'body resistance strengthening and evil eliminating', 'antibacterial and anti-inflammation'. Accordingly, the inventor of the invention determines the formula of the invention, and the compatibility mechanism is as follows: the astragalus membranaceus has the effects of tonifying qi, invigorating yang, expelling toxin, expelling pus, enhancing the immune function of the organism and improving the immune function; rhubarb, radix et rhizoma Rhei, purging pathogenic heat and promoting bowel movement, cooling blood and removing toxic substance, activating blood stasis and dredging channels; folium Isatidis, fructus forsythiae, and fructus Gardeniae have effects of cooling blood, removing toxic substances, relieving swelling, resolving hard mass, purging pathogenic fire, relieving restlessness, and inducing diuresis for treating stranguria; sweet wormwood herb, which is used for clearing deficiency heat, improving eyesight and relieving summer heat; honeysuckle flower, flos Lonicerae, heat-clearing and detoxicating, anti-inflammatory, tonifying deficiency and treating wind; radix bupleuri; the bupleurum can enhance the functions of natural killer cells and phagocytic function and improve the titer of virus specific antibodies; the houttuynia cordata has the effects of resisting bacteria and diminishing inflammation and improving the immunity; the mulberry leaves can disperse wind and heat, clear lung heat and moisten dryness, calm liver yang, clear liver and improve vision, cool blood and stop bleeding; the medicines are combined together, so that the traditional Chinese medicine can regulate yin and yang, treat both symptoms and root causes, balance yin and yang in physique and immunity, and achieve an ideal breeding state of 'having few diseases and being died'.
The preparation method comprises the following steps: weighing the raw materials according to a proportion, adding ethanol for extraction at room temperature, filtering, concentrating the filtrate under reduced pressure until the relative density at 60 ℃ is 1.1-1.3, then adding silicon dioxide, uniformly mixing, drying and crushing to obtain the traditional Chinese medicine compound preparation, wherein each 1000g of the traditional Chinese medicine compound preparation is equivalent to 2800-3000 g of crude drugs.
Preferably, the ethanol is 50v% ethanol.
Preferably, ethanol is extracted twice, filtered, and the two filtrates are combined; during the first extraction, the adding amount of ethanol is 5-8 times of the weight of the raw material medicine, and the extraction is carried out for 1.5-2 h; during the second extraction, the amount of ethanol added is 3-5 times of the weight of the raw materials, and the extraction lasts for 1-1.5 h.
The using method comprises the following steps: drinking water, 1000kg of water is added into each 1000g of the Chinese herbal compound preparation for 5-14 days.
The invention has the advantages and beneficial effects that: (1) the invention can effectively inhibit the blue ear virus; (2) the invention can well improve the immunity of the organism; (3) the traditional Chinese medicine preparation is prepared by adopting a traditional Chinese medicine extraction process, so that the effective components of the product are fully extracted, the product is easy to absorb after entering an organism, the clinical administration is convenient, the traditional Chinese medicine preparation is suitable for group administration, and manpower and material resources are saved; (4) the invention has definite medicine content, accurate administration and controllable quality; (5) the raw materials used in the invention are green products without drug resistance, and the food safety is not affected.
Detailed Description
The present invention will be further described with reference to the following specific examples. It should be understood that the following examples are illustrative only and are not intended to limit the scope of the present invention.
A traditional Chinese medicine compound preparation for inhibiting a blue ear virus is shown in Table 1.
Examples 1-3 and comparative example 2 a method for preparing a compound Chinese medicinal preparation: weighing each raw material medicine (total 3000 g) according to the proportion shown in the table 1, adding 50v% ethanol for extraction twice (during the first extraction, the adding amount of the ethanol is 6 times of the weight of the raw material medicines, extracting for 2 hours, during the second extraction, the adding amount of the ethanol is 3 times of the weight of the raw material medicines, extracting for 1 hour); filtering, combining the two filtrates, concentrating under reduced pressure to relative density of 1.2 at 60 deg.C, adding silicon dioxide, mixing, drying at 80 deg.C, pulverizing, and making into 1000g Chinese medicinal compound preparation.
The preparation method of the traditional Chinese medicine compound preparation in the comparative example 1 comprises the following steps: weighing each raw material medicine (total 3000 g) according to the proportion shown in the table 1, respectively carrying out superfine grinding (200 meshes), placing in a mixer, stirring for 30 minutes, and preparing 1000g of the traditional Chinese medicine compound preparation.
Inhibitory Effect against Tremella Fuciformis Virus
The method comprises the following steps:
(I) drugs and reagents
Test drugs: the Chinese herbal compound preparation prepared in examples 1, 2 and 3;
control drug
: the compound Chinese medicinal preparation prepared in comparative example 1;
control drug
: the compound Chinese medicinal preparation prepared in comparative example 2.
(II) test method
(1) And (3) experimental design: 100 piglets with respiratory symptoms, dyspnea, abdominal respiration, anorexia or abolition, body temperature rise to more than 40 ℃, diarrhea, rough hair, ataxia, progressive emaciation, eyelid edema and visible ear and body surface skin purplish are selected as test animals in a large-scale pig farm and are randomly divided into 5 groups, wherein each group comprises 20 piglets, and 3 test groups and 2 control groups. Blood is collected from the anterior vena cava of all experimental piglets to separate serum, the porcine reproductive and respiratory syndrome virus is detected, and the experimental drugs and the control drugs are fed to the pigs for detecting the positive porcine reproductive and respiratory syndrome virus for 2 weeks, which specifically comprises the following steps:
test group 1: 1000kg of water is added into every 1000g of the traditional Chinese medicine compound preparation in the embodiment 1, and water is freely drunk;
test group 2: 1000kg of water is added into every 1000g of the traditional Chinese medicine compound preparation in the embodiment 2, and water is freely drunk;
test group 3: 1000kg of water is added into every 1000g of the traditional Chinese medicine compound preparation in the embodiment 3, and water is freely drunk;
control group 1: 1000kg of water is added into 1000g of the traditional Chinese medicine compound preparation in the control example 1, and water is freely drunk;
control group 2: 1000kg of water is added into 1000g of the traditional Chinese medicine compound preparation in the control example 2, and water is freely drunk.
The anterior vena cava was bled at 1 week and 2 weeks after feeding, respectively, for the detection of the blue-ear virus, and the results are shown in table 2.
From the above table, it can be seen that: the test groups 1, 2 and 3 have obvious inhibiting effect on the blue ear virus, the negative conversion rate of 1 week is averagely 80.4 percent, is 33 percent higher than that of the control group 1, and is 38.3 percent higher than that of the control group 2; the negative conversion rate of 2 weeks is 96.5% on average, which is 28.1% higher than that of the control group 1, and 33.3% higher than that of the control group 2.
Claims (4)
1. A traditional Chinese medicine compound preparation for inhibiting a blue ear virus is characterized by being prepared from the following raw materials in parts by weight:
the composition is as follows: 10 parts of astragalus, 10 parts of rheum officinale, 10 parts of folium isatidis, 15 parts of fructus forsythiae, 5 parts of fructus gardeniae, 15 parts of sweet wormwood herb, 5 parts of honeysuckle, 10 parts of radix bupleuri, 5 parts of houttuynia cordata and 15 parts of folium mori, wherein the sum of the parts by weight of the raw materials is 100 parts;
the composition is two: 20 parts of astragalus membranaceus, 5 parts of rheum officinale, 15 parts of folium isatidis, 5 parts of fructus forsythiae, 10 parts of fructus gardeniae, 5 parts of sweet wormwood, 15 parts of honeysuckle, 5 parts of radix bupleuri, 10 parts of houttuynia cordata and 10 parts of folium mori, wherein the sum of the parts by weight of the raw materials is 100 parts;
composition (c): 15 parts of astragalus, 8 parts of rheum officinale, 10 parts of folium isatidis, 10 parts of fructus forsythiae, 8 parts of fructus gardeniae, 9 parts of sweet wormwood herb, 10 parts of honeysuckle, 10 parts of radix bupleuri, 10 parts of houttuynia cordata and 10 parts of folium mori, wherein the sum of the parts by weight of the raw materials is 100 parts;
the preparation method comprises the following steps: weighing the raw materials according to a proportion, adding ethanol for extraction at room temperature, filtering, concentrating the filtrate under reduced pressure until the relative density at 60 ℃ is 1.1-1.3, then adding silicon dioxide, uniformly mixing, drying and crushing to obtain the traditional Chinese medicine compound preparation, wherein each 1000g of the traditional Chinese medicine compound preparation is equivalent to 2800-3000 g of crude drugs.
2. A method for preparing the compound traditional Chinese medicine preparation for inhibiting the blue ear virus according to claim 1, which is characterized in that: weighing the raw materials according to a proportion, adding ethanol for extraction at room temperature, filtering, concentrating the filtrate under reduced pressure until the relative density at 60 ℃ is 1.1-1.3, then adding silicon dioxide, uniformly mixing, drying and crushing to obtain the traditional Chinese medicine compound preparation, wherein each 1000g of the traditional Chinese medicine compound preparation is equivalent to 2800-3000 g of crude drugs.
3. The method for preparing a compound Chinese medicinal preparation for inhibiting the blue ear virus according to claim 2, which is characterized in that: the ethanol is 50v% ethanol.
4. The method for preparing a compound Chinese medicinal preparation for inhibiting the blue ear virus according to claim 2 or 3, which is characterized in that: extracting twice, filtering, and combining the two filtrates; during the first extraction, the adding amount of ethanol is 5-8 times of the weight of the raw material medicine, and the extraction is carried out for 1.5-2 h; during the second extraction, the amount of ethanol added is 3-5 times of the weight of the raw materials, and the extraction lasts for 1-1.5 h.
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| CN111544570B (en) * | 2020-04-26 | 2023-05-05 | 河南省帝一方生物制药有限公司 | Antiviral preparation added with antibacterial peptide and preparation method thereof |
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