CN109045157A

CN109045157A - A kind of bactericidal composition of the extract containing Pudilan, mouthwash and preparation method thereof

Info

Publication number: CN109045157A
Application number: CN201811176684.5A
Authority: CN
Inventors: 郭思伟; 刘强; 陈婷; 蔡荣钦; 陈活记; 朱红霞
Original assignee: Guangdong Xinbao Pharmaceutical Technology Co Ltd
Current assignee: Guangdong Xinbao Pharmaceutical Technology Co Ltd
Priority date: 2018-10-10
Filing date: 2018-10-10
Publication date: 2018-12-21

Abstract

The invention discloses a kind of bactericidal compositions of extract containing Pudilan, which is characterized in that comprises the following components in parts by weight: 0.5-5 parts of Pudilan extract, 0.5-5 parts of Baical Skullcap root P.E, 2-8 parts of eugenol, 2-8 parts of Paeonol；The invention also discloses the mouthwashes and preparation method thereof for containing above-mentioned bactericidal composition.The present invention has significant bactericidal effect, and especially Streptococcus mutans are played with powerful killing effect, can effectively prevent decayed tooth；Also have the function of solving oral cavity excessive internal heat, bleeding gums, swelling and aching of gum and canker sore, and the preparation process of mouthwash of the invention is simple, it is easy to operate, be conducive to the industrialization production of mouthwash.

Description

A kind of bactericidal composition of the extract containing Pudilan, mouthwash and preparation method thereof

Technical field

The present invention relates to the bactericidal composition of a kind of bactericidal composition more particularly to a kind of extract containing Pudilan, gargle Water and preparation method thereof.

Background technique

Mouthwash is a kind of relatively conventional oral-cavity cleaning products, has many advantages, such as that use is simple, easy to carry, thus is gargled Saliva is very popular on the market, and mouthwash can solve many oral problems, such as periodontitis, saprodontia, oral peculiar smell, stomatitis Disease etc..Traditional mouthwash contains more alcohol or chemical synthesis substance, is unfavorable for the health of people, and contains Chinese medicine Mouthwash often ingredient is single, unobvious to the nursing efficacy in oral cavity, tooth and oral cavity cannot be made to play good protection.

And natural plant extracts have biological function abundant, such as emulsification, antibacterial, deodorization, hemostasis, resist It is scorching, prevent the effects of saprodontia and prevention canker sore, due to having strong synergistic function between vegetable active molecule, two It, can be fully by the life of respective bioactive molecule after kind or two or more plant extracts mix according to a certain percentage Object effect performs to ultimate attainment, achievees the effect that the plant extracts of one-component is incomparable, thus, plant extracts is closed Reason is effectively used in combination, and tends to reach unexpected oral care effect.

Summary of the invention

For overcome the deficiencies in the prior art, killing one of the objects of the present invention is to provide a kind of extract containing Pudilan Bacteria composition, the composition have significant bactericidal effect, especially Streptococcus mutans are played with powerful killing effect, Neng Gouyou Effect prevention decayed tooth；Also have the function of solving oral cavity excessive internal heat, bleeding gums, swelling and aching of gum and canker sore.

The second object of the present invention is to provide the sterilization group of the extract containing Pudilan containing an object of the present invention The mouthwash of object is closed, it has significant bactericidal effect, can effectively prevent decayed tooth, additionally it is possible to effectively solve oral cavity excessive internal heat, tooth The oral problems such as oulorrhagia, swelling and aching of gum and canker sore help oral cavity and tooth to keep good state, prevent harmful bacteria It is detrimental to health.

The third object of the present invention is to provide the preparation method of the mouthwash containing the second object of the present invention, the preparation side Method has simple process, and easy to operate feature is conducive to the industrialization production of mouthwash.

An object of the present invention adopts the following technical scheme that realization:

A kind of bactericidal composition of the extract containing Pudilan, which is characterized in that comprise the following components in parts by weight: Pudilan 0.5-5 parts of extract, 0.5-5 parts of Baical Skullcap root P.E, 2-8 parts of eugenol, 2-8 parts of Paeonol.

Further, it comprises the following components in parts by weight: 1 part of Pudilan extract, 1 part of Baical Skullcap root P.E, eugenol 5 Part, 5 parts of Paeonol.

Further, the preparation method of the bactericidal composition be by the Pudilan extract of formula ratio, Baical Skullcap root P.E, Eugenol and Paeonol are uniformly mixed to obtain the final product.

Further, the Pudilan extract is prepared by the component of following parts by weight: 20-60 parts of dandelion, 10-20 parts of viola mandshurica, 10-20 parts of Radix Isatidis.

Further, the Pudilan extract is prepared by the component of following parts by weight: 45 parts of dandelion, pale reddish brown 11.25 parts of Chinese violet, 16.9 parts of Radix Isatidis.

Further, the Pudilan extract the preparation method comprises the following steps: weighing the dandelion of formula ratio, viola mandshurica and plate Lan Gen adds 12 times of amount drinking water to decoct 2 times, 2 hours every time, filters；It is 1.05 that merging filtrate, which is concentrated into relative density, is centrifuged dense Contracting is to get Pudilan extract.

The second object of the present invention adopts the following technical scheme that realization:

A kind of mouthwash, which is characterized in that be prepared from the following parts by weight of the components:

800-1200 parts of 8-16 parts of bactericidal composition, sorbierite, the wood of Pudilan extract containing one of the object of the invention 100-500 parts of sugar alcohol, 100-500 parts of glycerol, 15-30 parts of essence, 5-20 parts of sodium benzoate, 100-500 parts of propylene glycol, poly- second two - 400 200-600 parts of alcohol, 1-10 parts of poloxamer188,20-60 parts of sodium monofluorophosphate, 10-40 parts of sodium citrate, menthol 1-6 parts, 50-150 parts of PEG- rilanit special, 5-25 parts of sodium chloride, 1-10 parts of Cetylpyridinium Chloride, deionized water 5000-15000 Part.

Further, it is prepared from the following parts by weight of the components:

12 parts of bactericidal composition, 1000 parts of sorbierite, 300 parts of xylitol, 300 parts of glycerol, the perfume of the extract containing Pudilan Smart 23 parts, 12 parts of sodium benzoate, 300 parts of propylene glycol, 400 parts of polyethylene glycol-400,5 parts of poloxamer188, sodium monofluorophosphate 40 parts, 25 parts of sodium citrate, 3 parts of menthol, 100 parts of PEG- rilanit special, 15 parts of sodium chloride, 5 parts of Cetylpyridinium Chloride, deionization 10000 parts of water.

The third object of the present invention adopts the following technical scheme that realization:

The preparation method of mouthwash described in the two of the object of the invention, which comprises the following steps:

The step of preparing mixture A: weighing the poloxamer188 of formula ratio, and deionized water, heating water bath, stirring is added Dissolution, obtains mixture A；

The step of preparing mixture B: by the glycerol of formula ratio, sorbierite, xylitol, sodium benzoate, sodium citrate, single fluorine Sodium phosphate and sodium chloride are added in mixture A, stirring and dissolving, and cooling obtains mixture B；

The step of preparing mixture C: propylene glycol, PEG- rilanit special and the polyethylene glycol-400 of formula ratio are weighed, is stirred It mixes uniformly, menthol is added and essence, stirring and dissolving obtain mixture C；

The step of preparing mouthwash finished product: the bactericidal composition of the extract containing Pudilan is added in mixture B, is stirred molten Solution, is added mixture C and Cetylpyridinium Chloride when stirring, and after stirring and dissolving, filters mouthwash finished product to obtain the final product.

Further, in described the step of preparing mixture A, the temperature of heating water bath is 55 DEG C -65 DEG C；The preparation is mixed In the step of closing object B, it is cooled to 25 DEG C -40 DEG C.

Compared with prior art, the beneficial effects of the present invention are:

1, the bactericidal composition of the extract of the invention containing Pudilan, from dandelion, Radix Isatidis and viola mandshurica these three Extract the Pudilan extract containing bioactive substance in crude drug, then with Baical Skullcap root P.E, eugenol and Paeonol Compounding, so that there are the bactericidal composition significant biocidal efficacies to have powerful killing effect especially to Streptococcus mutans, The composition is by the synergistic effect of each component, so that the original antibacterial anti-inflammatory of each component, clearing heat and detoxicating effect are superimposed And raising, the especially ratio of Pudilan extract, Baical Skullcap root P.E, eugenol and Paeonol be 1:1:5:5 when, four kinds Synergistic effect between drug is the most obvious, and antibacterial anti-inflammatory, clearing heat and detoxicating effect are the most significant.

2, the mouthwash of offer of the invention is compounded using plurality of Chinese, and natural environmental-protective has no toxic side effect, using, It is easy to carry, mouthfeel is mild, it is comfortable, do not stimulate, securely and reliably, the health that can effectively safeguard oral cavity and tooth is used for a long time； And the eugenol that uses of the present invention and main component that Paeonol is cloves and the root bark of tree peony respectively, the cloves used with the prior art Extract is compared with Cortex Moutan extract, has the advantages that pharmacological activity is high, quality controllable, effect is more significant.

3, the present invention provides mouthwash, is compounded by the bactericidal composition and other components of the extract containing Pudilan, so that The mouthwash has significant bactericidal effect, can effectively prevent decayed tooth, harmful bacteria is prevented to be detrimental to health；Can also have Effect solves oral cavity excessive internal heat, bleeding gums, and the oral problems such as swelling and aching of gum and canker sore help oral cavity and tooth to keep good State；And mouthfeel it is mild, it is comfortable, do not stimulate, it is traditional Chinese medicinal components safety, mild, be suitable for being used for a long time.

4, the preparation method of mouthwash provided by the invention can be extracted sufficiently and Baical Skullcap root P.E, Pudilan is protected to mention Take object, eugenol, biologically active effective component in Paeonol, it is ensured that the effective component in mouthwash can give full play to The effect of bactericidal antiphlogistic, to solve oral cavity excessive internal heat, bleeding gums, the oral problems such as swelling and aching of gum and canker sore are effectively tieed up Hold oral health；And the preparation method has simple process, and easy to operate feature is conducive to industrialization production.

Detailed description of the invention

Fig. 1 is the result figure of No. 12 culture mediums in escherichia coli test；

Fig. 2 from left to right, be followed successively by from top to bottom escherichia coli test in No. 1, No. 2, No. 10 and No. 11 culture medium Result figure；

Fig. 3 from left to right, is followed successively by the result figure of the 3-9 culture medium in escherichia coli test from top to bottom；

Fig. 4 is the result figure of No. 12 culture mediums in staphylococcus aureus test；

Fig. 5 is the result figure of No. 2 culture mediums in staphylococcus aureus test；

Fig. 6 from left to right, be followed successively by from top to bottom staphylococcus aureus test in No. 1, No. 3, No. 4, No. 5, No. 7,8 The result figure of number culture dish；

Fig. 7 is the result figure of No. 12 culture mediums in Candida albicans test；

Fig. 8 from left to right, is followed successively by the result figure of the 1-10 culture medium in Candida albicans test from top to bottom；

Fig. 9 is the result figure of No. 12 culture mediums in Streptococcus mutans test；

Figure 10 from left to right, is followed successively by the result figure of the 1-11 culture medium in Streptococcus mutans test from top to bottom.

Specific embodiment

In the following, being described further in conjunction with specific embodiment to the present invention, it should be noted that is do not collided Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.It is adopted Equipment and raw material etc. are commercially available or commonly used in the art.Method in following embodiments, such as nothing are especially said It is bright, it is the conventional method of this field.

A kind of bactericidal composition of the extract containing Pudilan, comprises the following components in parts by weight: Pudilan extract 0.5-5 Part, 0.5-5 parts of Baical Skullcap root P.E, 2-8 parts of eugenol, 2-8 parts of Paeonol.

It as further embodiment, comprises the following components in parts by weight: 1 part of Pudilan extract, Baical Skullcap root P.E 1 Part, 5 parts of eugenol, 5 parts of Paeonol.

Wherein, Pudilan extract is to extract and obtain from 3 kinds of medicinal materials such as dandelion, viola mandshurica, Radix Isatidis；With clear The effect of thermal detoxification, anti-inflammation detumescence.

Dandelion herb contains taraxasterol, choline, synanthrin, caffeic acid, pectin, taraxol, β-amyrin, beans steroid Alcohol, cupreol, taraxerol, taraxacin, taraxacin and vitamin A, B, C etc..Wherein, dandelion it is effective at Divide includes caffeic acid and Cichoric acid；Dandelion has clearing heat and detoxicating, the effect of diuresis dissipating bind.

Radix Isatidis contains indigo, indigo red, Anthraquinones, cupreol, clionasterol and a variety of amino acid: arginine, paddy Propylhomoserin, tyrosine, proline, valine, γ-aminobutyric acid；Also contain potassium myronate, indigo, couroupitine A, 1- thiocyanic acid -2- hydroxyl Base butyl- 3- alkene, gland glucoside, palmitinic acid, sucrose and the proteoglycan containing 12% amino acid.Radix Isatidis has clearing heat and detoxicating, cool blood, Relieving sore-throat, antiviral effect.

Viola mandshurica is the drying herb of violaceae plant viola mandshurica.

Baical Skullcap root P.E the preparation method comprises the following steps: weigh the radix scutellariae of formula ratio, add 60% ethyl alcohol to be heated to reflux 3 times, 2 is small every time When, filtering；It is 1.05 that merging filtrate recycling ethyl alcohol, which is concentrated into relative density, and centrifugal concentrating is to get Baical Skullcap root P.E.

Radix scutellariae mainly contains flavone compound: scutelloside is the principle active component of radix scutellariae, molecular formula C₂₁H₁₈O₁₁, point Son amount be 446.35, be yellow crystal, 223 DEG C of fusing point；Radix scutellariae has the effect of antimicrobial antiphlogistic, heat-clearing and damp-drying drug, purging fire for removing toxin；

Eugenol has strong cloves fragrance, is mainly used for antibacterial；

Paeonol has the function of antipyretic-antalgic, anti-inflammatory, antibacterial, anti-oxidant and inhibition allergy.

As further embodiment, the preparation method of bactericidal composition is by Pudilan extract, the Huang of formula ratio A kind of reed mentioned in ancient books extract, eugenol and Paeonol are uniformly mixed to obtain the final product.

As further embodiment, Pudilan extract is prepared by the component of following parts by weight: dandelion 20-60 parts, 10-20 parts of viola mandshurica, 10-20 parts of Radix Isatidis.

As further embodiment, Pudilan extract is prepared by the component of following parts by weight: dandelion 45 parts, 11.25 parts of viola mandshurica, 16.9 parts of Radix Isatidis.

As further embodiment, Pudilan extract the preparation method comprises the following steps: weighing the dandelion of formula ratio, pale reddish brown Chinese violet and Radix Isatidis add 12 times of amount drinking water to decoct 2 times, 2 hours every time, filter；Merging filtrate is concentrated into relative density 1.05, centrifugal concentrating is to get Pudilan extract.

A kind of mouthwash, is prepared from the following parts by weight of the components:

800-1200 parts of 8-16 parts of bactericidal composition, sorbierite, xylitol 100-500 containing above-mentioned Pudilan extract Part, 100-500 parts of glycerol, 15-30 parts of essence, 5-20 parts of sodium benzoate, 100-500 parts of propylene glycol, polyethylene glycol-400 200- 600 parts, 1-10 parts of poloxamer188,20-60 parts of sodium monofluorophosphate, 10-40 parts of sodium citrate, 1-6 parts of menthol, PEG- hydrogen Change castor oil 50-150 parts, 5-25 parts of sodium chloride, 1-10 parts of Cetylpyridinium Chloride, 5000-15000 parts of deionized water.

As further embodiment, it is prepared from the following parts by weight of the components:

The preparation method of above-mentioned mouthwash, which comprises the following steps:

As further embodiment, in the step of preparing mixture A, the temperature of heating water bath is 55 DEG C -65 DEG C；System In the step of standby mixture B, it is cooled to 25 DEG C -40 DEG C.

Embodiment 1-3:

A kind of mouthwash, is prepared from the following components:

The bactericidal composition of the extract containing Pudilan, xylitol, glycerol, essence, sodium benzoate, propylene glycol, gathers sorbierite Ethylene glycol -400, poloxamer188, sodium monofluorophosphate, sodium citrate, menthol, PEG- rilanit special, sodium chloride, western pyrrole Oronain, deionized water.

Wherein, the bactericidal composition of the extract containing Pudilan the preparation method comprises the following steps: 1 part of Pudilan extract, radix scutellariae are mentioned Take 1 part of object, 5 parts of eugenol, 5 parts of Paeonol, be uniformly mixed to get；At this point, Pudilan extract, Baical Skullcap root P.E, eugenol Ratio with Paeonol is 1:1:5:5；

Pudilan extract the preparation method comprises the following steps: weighing 45 parts of dandelion, 11.25 parts of viola mandshurica and Radix Isatidis 16.9 Part, add 12 times of amount drinking water to decoct 2 times, 2 hours every time, filters；It is 1.05 that merging filtrate, which is concentrated into relative density, is centrifuged dense Contracting is to get Pudilan extract；

The content (calculating by weight) of mouthwash each component is shown in Table 1.

The content of the mouthwash each component of 1 embodiment 1-3 of table:

The preparation method of above-mentioned mouthwash, comprising the following steps:

The step of preparing mixture A: weighing the poloxamer188 of formula ratio, deionized water is added, in 60 DEG C of Shui Zhongshui Bath heating, stirring and dissolving, obtain mixture A；

The step of preparing mixture B: by the glycerol of formula ratio, sorbierite, xylitol, sodium benzoate, sodium citrate, single fluorine Sodium phosphate and sodium chloride are added in mixture A, stirring and dissolving, and temperature is down to 40 DEG C hereinafter, obtaining mixture B；

Verify embodiment:

Mouthwash prepared by testing example 2 is to escherichia coli, staphylococcus aureus, Candida albicans and variation chain The fungistatic effect of coccus passes through the beneficial effect of the following tests mouthwash described come the present invention is further explained.

1 test strain:

Escherichia coli CMCC (B) 44102；Staphylococcus aureus CMCC (B) 26003；Candida albicans (read by white Pearl bacterium) CMCC (F) 98001；Streptococcus mutans ATCC 25175；Above four kinds of bacterial strains are limited purchased from the triumphant microorganism science and technology of Guangdong ring Company.

2 test reagents:

Silica, sorbierite, deionized water, glycerine, polyethylene glycol-400, K-12 powder, essence, xanthan gum, hydroxyl second It is base cellulose, sodium monofluorophosphate, saccharin sodium, menthol, peppermint oil, sodium benzoate, 1% brilliant blue solution, methanol, phosphoric acid, sterile Physiological saline, agar powder.

3 test culture mediums:

LB nutrient agar (LB Nutrient Agar) 250g；Columbia blood agar base (improvement) (Modified Columbia Blood Agar Base)250g；MH meat soup (MH Broth) 250g；Streptococcus mutans culture medium 250g.

4 test apparatuses:

Electric blender (Changzhou Guo Yu instrument manufacturing Co., Ltd), KH-100DE type numerical control ultrasonic cleaner (Kunshan He Chuan ultrasonic instrument Co., Ltd), Centrifuge 5804R (Guangzhou He Zhong Biotechnology Co., Ltd), Agilent1290 (Agilent company of the U.S.), UV2PCR Workstation (UVP), insulating box, bacterial cultures bacterium operating room (Chinese materia medica preparation Teaching and research room provides), high steam pot, 10 μ l oeses etc..

5 test methods and result

The bacteriostatic test of 5.1 pairs of escherichia coli:

5.1.1 the culture medium of the mouthwash containing Pudilan is prepared:

Accurate measuring 4.0gLB nutrient agar after adding 100ml distilled water to dissolve by heating, is added in clean triangular flask 1.5g agar powder, until completely dissolved, 121 DEG C of high pressure sterilization 15min are spare.

Take the test tube of 12 clean sterilizings, label 1-12.The mouthwash 5ml for measuring embodiment 2 is tried in No. 1 test tube and No. 2 Guan Zhong carries out two-fold dilution to No. 2 test tubes with sterile saline, is made into the dilution of 1:2；5mL liquid is taken from No. 2 test tubes In No. 3 test tubes, carry out two-fold dilution, be configured to the dilution of 1:4, and so on, 1-11 test tube be successively configured to 1:1, The water diluent of gargling of 1:2,1:4,1:8,1:16,1:32,1:64,1:128,1:256,1:512,1:1024, No. 12 test tube For sterile saline, as blank control.

It takes above-mentioned test solution 1ml in the culture dish of cleaning sterilizing, is uniformly mixed with agar solution with the ratio of 1:9, number point Not Wei 1~12, be cooled to solidification.By the culture medium solidified sealing, it is inverted in overnight incubation in 37 DEG C of insulating box, is observed Whether living contaminants are had.

5.1.2 it is inoculated with:

On inspection, all culture mediums are dilute by escherichia coli freeze-dried powder sterile saline not by living contaminants It releases, using plate streak, takes bacterium solution to be inoculated on each culture medium with the aseptic inoculation ring that inoculum concentration is 10ul.It is placed in Bacterial growth situation is observed after cultivating 24 hours in 37 DEG C of insulating box, is compared with blank control, and whether there is or not bacterium lifes for observation It is long, using the minimum diluted concentration of no bacterial growth as minimal inhibitory concentration, i.e. MIC.

5.1.3 test result:

All culture dishes have different degrees of bacterium colony growth, wherein the clump count in 3-6 culture dish is considerably less than sky The clump count of No. 12 culture dishes of white control, although can not determine MIC, but it is also possible to find out, the culture medium pair containing mouthwash Escherichia coli has certain inhibiting effect；Detailed results are shown in Table 1, and the bacterium colony situation on culture dish is referring to attached drawing 1-3.

The bacteriostatic test of 5.2 pairs of staphylococcus aureuses:

5.2.1 the culture medium of the mouthwash containing Pudilan is prepared:

Accurate measuring 4.4g Columbia Blood Agar after adding 100ml distilled water to dissolve by heating, adds in clean triangular flask Enter 1.5g agar powder, until completely dissolved, when being cooled to 50 DEG C or so, the sterile de- fibre of 5ml is added in 121 DEG C of high pressure sterilization 15min Sheep blood is tieed up, is mixed, it is spare.

5.2.2 it is inoculated with:

On inspection, all culture mediums dilute staphylococcus aureus with sterile saline not by living contaminants, Using plate streak, bacterium solution is taken to be inoculated on each culture medium with the aseptic inoculation ring that inoculum concentration is 10ul.It is placed in 37 DEG C insulating box in cultivate 24 hours after observe bacterial growth situation, be compared with blank control, observation whether there is or not bacterial growth, Using the minimum diluted concentration of no bacterial growth as minimal inhibitory concentration, i.e. MIC.

5.2.3 test result:

All culture dishes have different degrees of bacterium colony growth, the culture medium of culture medium and blank control containing mouthwash Clump count can not also determine MIC without significant difference, and detailed results are shown in Table 1, and the bacterium colony situation on culture dish is referring to attached drawing 4-6.

The bacteriostatic test of 5.3 pairs of Candida albicans:

5.3.1 the culture medium of the mouthwash containing Pudilan is prepared:

After adding 100ml distilled water to dissolve by heating, 1.5g fine jade is added in clean triangular flask in accurate measuring 2.1gMH meat soup Cosmetics, until completely dissolved, 121 DEG C of high pressure sterilization 15min are spare.

5.3.2 it is inoculated with:

On inspection, all culture mediums dilute Candida albicans with sterile saline not by living contaminants, use Plate streak takes bacterium solution to be inoculated on each culture medium with the aseptic inoculation ring that inoculum concentration is 10ul.It is placed in 37 DEG C Bacterial growth situation is observed after cultivating 2-3 days in insulating box, is compared with blank control, and whether there is or not bacterial growths for observation, with nothing The minimum diluted concentration of bacterial growth is minimal inhibitory concentration, i.e. MIC.

5.3.3 test result:

All culture dishes have different degrees of bacterium colony growth, wherein the clump count in 1-3 culture dish is considerably less than sky The clump count of No. 12 culture dishes of white control, although can not determine MIC, but it is also possible to find out, the culture medium pair containing mouthwash Candida albicans has certain inhibiting effect；Detailed results are shown in Table 1, and the bacterium colony situation on culture dish is referring to attached drawing 7-8.

The bacteriostatic test of 5.4 pairs of Streptococcus mutans:

5.4.1 the culture medium of the mouthwash containing Pudilan is prepared:

After accurate measuring 3.7g Streptococcus mutans culture is based on 100ml distilled water heating for dissolving in clean triangular flask, is added, 1.5g agar powder is added, until completely dissolved, 121 DEG C of high pressure sterilization 15min are spare.

It takes above-mentioned test solution 1ml in the culture dish of cleaning sterilizing, is uniformly mixed with agar solution with the ratio of 1:9, number point Not Wei 1~12, be cooled to solidification.By the pastille culture medium solidified sealing, it is inverted in overnight incubation in 37 DEG C of insulating box, Living contaminants are seen whether.

5.4.2 it is inoculated with:

On inspection, all culture mediums are dilute by Streptococcus mutans freeze-dried powder sterile saline not by living contaminants It releases, using plate streak, takes bacterium solution to be inoculated on each culture medium with the aseptic inoculation ring that inoculum concentration is 10ul.It will training Feeding ware is put into anaerobism bag, is put into oxygen indicator and micro- aerobic production gas bag, sealing.It is small to be placed in culture 48 in 37 DEG C of insulating box When after observe bacterial growth situation, be compared with blank control, observation is dilute with the minimum of no bacterial growth whether there is or not bacterial growth Releasing concentration is minimal inhibitory concentration, i.e. MIC.

5.4.3 test result:

Sterile length of being born on No. 1-4 culture medium containing mouthwash, the clump count on 5-6 culture medium is also less than blank pair According to the clump count on No. 12 culture mediums, thus, it is possible to determine, the concentration of No. 4 culture mediums can be considered MIC, and detailed results are shown in Table 1, Bacterium colony situation on culture dish is referring to attached drawing 9-10.

Table 1 is that the bacterium colony of four kinds of bacteriums on 1-12 culture dish situation occurs:

In table 1 ,+: representative has bacterium colony growth；: represent sterile length of being born.

Staphylococcus aureus, escherichia coli, Candida albicans and Streptococcus mutans are all common pathogenic bacteria, are gargled Saliva has a degree of inhibiting effect to above-mentioned four kinds of bacteriums, wherein the inhibitory effect to Streptococcus mutans is significant；And become Different streptococcus is the main pathogenic fungi for leading to saprodontia, and the hair that the mouthwash containing Pudilan is capable of effectively pre- anti-caries is used for a long time It is raw, while it being also adjustable intraoral colony balance, the generation of human oral cavity disease is reduced, is also conducive to people and keeps body strong Health.

The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto, The variation for any unsubstantiality that those skilled in the art is done on the basis of the present invention and for belong to the present invention wanted Seek the range of protection.

Claims

1. a kind of bactericidal composition of extract containing Pudilan, which is characterized in that comprise the following components in parts by weight: Pudilan mentions Take 0.5-5 parts of object, 0.5-5 parts of Baical Skullcap root P.E, 2-8 parts of eugenol, 2-8 parts of Paeonol.

2. the bactericidal composition of the extract according to claim 1 containing Pudilan, which is characterized in that including following parts by weight Component: 1 part of Pudilan extract, 1 part of Baical Skullcap root P.E, 5 parts of eugenol, 5 parts of Paeonol.

3. the bactericidal composition of the extract according to claim 1 or 2 containing Pudilan, which is characterized in that

The preparation method of the bactericidal composition is by Pudilan extract, Baical Skullcap root P.E, eugenol and the root bark of tree peony of formula ratio Phenol is uniformly mixed to obtain the final product.

4. the bactericidal composition of the extract according to claim 1 containing Pudilan, which is characterized in that the Pudilan extracts Object is prepared by the component of following parts by weight: 20-60 parts of dandelion, 10-20 parts of viola mandshurica, 10-20 parts of Radix Isatidis.

5. the bactericidal composition of the extract according to claim 4 containing Pudilan, which is characterized in that the Pudilan extracts Object is prepared by the component of following parts by weight: 45 parts of dandelion, 11.25 parts of viola mandshurica, 16.9 parts of Radix Isatidis.

6. the bactericidal composition of the extract according to claim 5 containing Pudilan, which is characterized in that the Pudilan extracts Object the preparation method comprises the following steps: weigh the dandelion of formula ratio, viola mandshurica and Radix Isatidis, add 12 times of amount drinking water to decoct 2 times, every time 2 hours, filtering；It is 1.05 that merging filtrate, which is concentrated into relative density, and centrifugal concentrating is to get Pudilan extract.

7. a kind of mouthwash, which is characterized in that be prepared from the following parts by weight of the components:

100-500 parts of 800-1200 parts of 8-16 parts of bactericidal composition, sorbierite, xylitol, the glycerol of the extract containing Pudilan 100-500 parts, 15-30 parts of essence, 5-20 parts of sodium benzoate, 100-500 parts of propylene glycol, 200-600 parts of polyethylene glycol-400, 1-10 parts of poloxamer188,20-60 parts of sodium monofluorophosphate, 10-40 parts of sodium citrate, 1-6 parts of menthol, PEG- hydrogenated castor 50-150 parts oily, 5-25 parts of sodium chloride, 1-10 parts of Cetylpyridinium Chloride, 5000-15000 parts of deionized water.

8. mouthwash according to claim 7, which is characterized in that be prepared from the following parts by weight of the components:

12 parts of bactericidal composition, 1000 parts of sorbierite, 300 parts of xylitol, 300 parts of the glycerol, essence 23 of the extract containing Pudilan Part, 12 parts of sodium benzoate, 300 parts of propylene glycol, 400 parts of polyethylene glycol-400,5 parts of poloxamer188, sodium monofluorophosphate 40 Part, 25 parts of sodium citrate, 3 parts of menthol, 100 parts of PEG- rilanit special, 15 parts of sodium chloride, 5 parts of Cetylpyridinium Chloride, deionized water 10000 parts.

9. the preparation method of mouthwash according to claim 7 or 8, which comprises the following steps:

The step of preparing mixture A: weighing the poloxamer188 of formula ratio, is added deionized water, heating water bath, stirring and dissolving, Obtain mixture A；

The step of preparing mixture B: by the glycerol of formula ratio, sorbierite, xylitol, sodium benzoate, sodium citrate, single fluorophosphoric acid Sodium and sodium chloride are added in mixture A, stirring and dissolving, and cooling obtains mixture B；

The step of preparing mixture C: weighing propylene glycol, PEG- rilanit special and the polyethylene glycol-400 of formula ratio, and stirring is equal It is even, menthol is added and essence, stirring and dissolving obtain mixture C；

The step of preparing mouthwash finished product: the bactericidal composition of the extract containing Pudilan being added in mixture B, stirring and dissolving, It is added mixture C and Cetylpyridinium Chloride when stirring, after stirring and dissolving, filters up to mouthwash finished product.

10. the preparation method of mouthwash according to claim 9, which is characterized in that described the step of preparing mixture A In, the temperature of heating water bath is 55 DEG C -65 DEG C；In described the step of preparing mixture B, it is cooled to 25 DEG C -40 DEG C.