KR101743508B1 - Mouth cleaner comprising citrus or lime extract and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a pharmaceutical composition comprising 1 to 4% by weight of an extract extracted from citrus or lime, 40 to 60% by weight of a D-sorbitol solution, 2.0 to 3.0% by weight of vegetable glycerin, 2.0 to 10.0% by weight of poloxamer 407, 0.2% by weight of sodium fluoride (sodium fluoride) and 0.25% by weight of cetylpyridinium chloride, 0.20% by weight of mint scent, 0.00014% by weight of blue No. 1, 0.20% by weight of sodium benzoate, 0.25-0.50% by weight, xylitol 0.10-0.50% by weight, and remaining purified water, and a method for producing the same.

Description

TECHNICAL FIELD [0001] The present invention relates to an oral cleanser comprising citrus or lime extract, and a method for preparing the same. [0002]

The present invention relates to an oral cleanser comprising citrus or lime extract and a method for producing the same.

Oral diseases are 80% of adults in Korea and about 90% of elderly people over 60 are suffering from chronic diseases. Gingival disease is the third most common foreign disease, and its incidence is high and it is reported to increase every year.

Periodontal disease is closely related to the occurrence of systemic chronic diseases. The importance of eco-friendly and human-friendly natural materials is increasing day by day in the development of health supplements and hygiene products related to the medical and dental medicine fields. Especially, in order to prevent and improve periodontal diseases, Interest in phyto-materials is increasing in the materials.

Although 80% of domestic adults and 90% of the silver generation age are suffering from chronic oral diseases, the development and commercialization of auxiliary products that can be managed in advance preventive measures have not been made.

Functional detergents currently on the market include functional inhibition of plaque inhibition, inhibition of noxious bacteria in oral cavity, improvement of bad breath, and whitening effect. However, most products that do not take into account age, Inflammation is often exacerbated, and there is a demand for a product development technology to replace it.

It is an object of the present invention to provide a hypodermic, functional natural mouthwash using an extract extracted from citrus or lime and a method for providing the same.

In one embodiment, the invention provides an oral cleanser comprising an extract from citrus or lime.

In another embodiment, the present invention provides a composition comprising 1 to 4% by weight of an extract from citrus or lime, 40 to 60% by weight of D-sorbitol, 2.0 to 3.0% by weight of vegetable glycerin, 2.0 to 10.0% by weight of Pollockamer 407, ) And sodium citrate (0.005% by weight), sodium fluoride (sodium fluoride) and cetylpyrinium chloride 0.22% by weight, mint scent 0.20% by weight, blue No. 1 0.00014% by weight, sodium benzoate (sodium benzonate) 0.20% by weight %, Enzyme treated stevia 0.25-0.50% by weight, xylitol 0.10-0.50% by weight, and the remaining purified water.

In yet another embodiment, the present invention provides a method of preparing a composition comprising adding 1 to 4% by weight of an extract extracted from citrus or lime to a solvent and adding 40 to 60% by weight of a D-sorbitol solution, 2.0 to 3.0% by weight of vegetable glycerin, , Sodium citrate (sodium fluoride) and cetylpyridinium chloride 0.22% by weight, mint scent 0.20% by weight, blue No. 1 0.00014% by weight, sodium benzoate (sodium fluoride) 0.20% by weight of sodium benzoate (sodium benzoate), 0.25 to 0.50% by weight of enzyme-treated Stevia, 0.10 to 0.50% by weight of xylitol, and the remaining purified water to the solvent.

At this time, the citrus can be a hanabong. At this time, the extract extracted from citrus or lime may be essential oil or Floral Water.

The present invention provides a hypodermic, functional natural mouthwash using an extract extracted from citrus or lime and a method for providing the same.

In addition, the oral cleaning agent of the present invention contains extracts extracted from citrus or lime, and has antioxidative activity, inhibition and sterilization of oral harmful bacteria, and improvement of dental calculus as main effects.

Further, the oral cleanser of the present invention has a good color, flavor, and taste, and is lukewarm / clean after gargle, has improved bad breath and has a refreshing effect upon use.

Figure 1 shows the electron donating ability according to DPPH radical measurement of each sample.
2 shows SOD-like activity values of the respective samples.
3 is a graph showing a total polyphenol content value of each sample.
Fig. 4 shows the flavonoid content of each sample.
Figure 5 shows the clear zone size of oral cleanser by concentration for C. albicans .
Figure 6 shows the clear zone size for each concentration of oral detergent against S. mutans .
Figure 7 shows the clear zone size for each concentration of oral cleanser for S. sobrinus .
Figure 8 shows cell viability for normal oral cells.
9 shows the ability to inhibit proliferation of oral cancer cells.
FIG. 10 shows analysis of changes in the content of halitosis substances in (a) and (b) before use of the oral cleaning agent.
Fig. 11 shows analysis of changes in the content of halitosis substances in (a) and (b) before use of the oral cleanser.

Hereinafter, some embodiments of the present invention will be described in detail with reference to exemplary drawings. It should be noted that, in adding reference numerals to the constituent elements of the drawings, the same constituent elements are denoted by the same reference symbols as possible even if they are shown in different drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

In the present specification, the percentages may be% by volume or, if necessary,% by weight, unless otherwise specified.

Oral has been considered as one of the important causes of oral and systemic diseases caused by pathogenic infectious strains and resident bacteria by providing a very easy environment for various microorganisms. In order to improve oral health by controlling the growth conditions of oral microorganisms, various researches and developments have been made. However, since the abuse and abuse of medicines and the resistance of medicines to the infectious organisms have become serious problems, .

The present invention relates to a pharmaceutical composition comprising 1 to 4% by weight of an extract extracted from citrus or lime, 40 to 60% by weight of a D-sorbitol solution, 2.0 to 3.0% by weight of vegetable glycerin, 2.0 to 10.0% by weight of poloxamer 407, 0.2% by weight of sodium fluoride (sodium fluoride) and 0.25% by weight of cetylpyridinium chloride, 0.20% by weight of mint scent, 0.00014% by weight of blue No. 1, 0.20% by weight of sodium benzoate, 0.25-0.50% by weight, xylitol 0.10-0.50% by weight, and the remaining purified water.

The extracts extracted from citrus or lime in this specification should be interpreted to include extracts from citrus, extracts from lime, or mixtures thereof.

Citrus (citrus) is a generic term for the evergreen arborescens belonging to the mulberry tree and the citrus fruit. Citrus are sensitive to temperature, and it is difficult to cultivate even if the temperature conditions in winter are inadequate, no matter how good the other cultivation conditions are. The country of origin is concentrated in the subtropical region of Asia's tropics, but mostly in the southeastern part of the Asian continent.

Citrus peel has a vesicle containing essential oil, and this essential oil is widely used as a perfume. The main component is d-limonene in an amount of 90% or more, and the major direction components include capric aldehyde, capryl aldehyde, citral, d-linalool and nonyl alcohol. In addition, formic acid, acetic acid, capric acid, As esters of prylic acid, there are coumarin derivatives such as umbelliferone, opacene and the like.

Halabong refers to the mulberry tree mandarin orange and mandarin orange. Halabong is a breed of citrus fruit that was cultivated and cultivated in the citrus department of Japan Agriculture Forestry Experiment Station in 1972. Halabong is eaten raw by removing the skin, processed by processed foods such as Halabong tea, marmalade [jelly-shaped jam made with citrus peels and sugar into the pulp].

Lime (Citrus aurantifolia) is an evergreen shrub with a dicotyledonous plant. It grows widely in the subtropical and tropical regions because it is cold in the northeast of India and northern Myanmar and Malaysia. The flesh of the fruit is yellowish green, soft, juicy, sour, more sour and sweet than lemon. Lime is divided into Acid lime containing citric acid and Sweet lime without citric acid.

In yet another embodiment, the present invention provides a method of preparing a composition comprising adding 1 to 4% by weight of an extract extracted from citrus or lime to a solvent and adding 40 to 60% by weight of a D-sorbitol solution, 2.0 to 3.0% by weight of vegetable glycerin, , Sodium citrate (sodium fluoride) and cetylpyridinium chloride 0.22% by weight, mint scent 0.20% by weight, blue No. 1 0.00014% by weight, sodium benzoate (sodium fluoride) 0.20% by weight of sodium benzoate (sodium benzoate), 0.25 to 0.50% by weight of enzyme-treated Stevia, 0.10 to 0.50% by weight of xylitol, and the remaining purified water to the solvent.

At this time, the citrus can be a hanabong. At this time, the extract extracted from citrus or lime may be essential oil or Floral Water. Floral Water is a by-product of the hydrosol state that occurs during the extraction of essential oils.

A certain amount of citrus or lime, for example, 10 kg of perilla, is thoroughly rinsed with water, and then put in a tank net of a known essential oil extracting apparatus and subjected to extraction at 110 ° C for about 8 hours. At this time, the separated floral water can be separated at the same time during the extraction of the essential oil.

The citrus or lime extract may be added to the solvent in an amount of 1 to 4% by weight as described above, followed by the addition of other additives to the oral care cleanser.

Hereinafter, a method for producing an oral cleanser comprising extracts from citrus or lime and a composition thereof will be described in detail with reference to specific examples, but it should be borne in mind that the scope of the present invention is not limited by these embodiments.

Hereinafter, the properties and efficacy of the extracts extracted from citrus or lime will be examined. In this case, hara bong is distinguished from citrus, and the characteristics and efficacy of the extract are examined. In addition, the following extracts or essential oils and floral water are used in combination because they are identical in terms of characteristics and efficacy.

I. Basic Benefits of Citrus or Lime

1. Antioxidant Activity Analysis of Extracts and Oral Detergent Compositions

1) DPPH radical scavenging ability

The active oxygen that is generated and accumulated during the metabolism of the cells that constitute the gum tissue is harmful to the cells and causes premature aging of the gums. Therefore, in this experiment, we have selected a plant material capable of inhibiting such active oxygen, and conducted experiments to use such a material as a natural material for oral cleaning agent.

The free radical scavenging activity of the sample was measured using the DPPH method (Brand-Wiliams et al., 1995). DPPH (1,1-diphenyl-2-picryl-hydrazyl) is a dark purple color and itself is a radical of nitrogen center, which is stabilized by the nonuniformization of radical electrons. 100 μl of each sample was added with 100 μl of 100 μM DPPH solution dissolved in ethanol to make 200 μl of the final reaction solution. After reacting for a certain period of time, the absorbance was measured at 517 nm using an ELISA reader, and the decrease of absorbance by reduction of DPPH was examined. The free radical scavenging activity was determined by comparing the values of control and treatment groups.

As a result, the antioxidant activity was high in the order of lime floral water, hara bong floral water, citrus extract, oral cleaning agent (comparative rogurin product) composition at 50% dilution concentration, citrus extract was 24.28% at 50 ㎍ / , 21.18% at 25 ㎍ / ㎖ and 18.15% at 13 ㎍ / ㎖, respectively. The composition showed 14.96% at 50% and 6.66% at 25%. As shown in FIG. 1, DPPH radical scavenging activity increased proportionally with increasing concentration of extract. Table 1 shows DPPH radical measurements at 517 nm.

Halabang Floral Water 50% 37.13 35.92 36.32 36.46 25% 21.46 22.78 22.37 22.20 13% 14.39 16.61 13.38 14.79 6% 13.27 16.61 14.59 14.82 3% 12.77 11.56 9.03 11.12 Lime floral water 50% 82.28 83.07 83.87 83.07 25% 48.62 62.06 24.93 45.20 13% 45.64 23.43 24.93 31.33 6% 30.20 21.74 23.03 24.99 3% 30.80 23.63 19.05 24.49 Citrus extract 500 μg 22.55 26.65 23.65 24.28 250 g 18.25 25.55 19.75 21.18 125 g 13.04 22.55 18.85 18.15 61 쨉 g 7.54 24.45 18.65 16.88 30 ㎍ 4.54 15.54 16.94 12.34 Oral cleanser composition 50% 14.27 13.28 14.27 13.94 25% 6.13 6.03 7.81 6.66 13% (0.03) 1.36 0.46 0.60 6% 0.00 0.00 0.00 0.00 3% 0.00 0.00 0.00 0.00

Figure 1 shows the electron donating ability according to DPPH radical measurement of each sample. At this time, the electron donating ability EDA (%) = (control OD-sample OD) / controlO.Dx100.

   2) SOD-like activity

  Superoxide dismutase (SOD) - like activity was measured by Marklund & Marklund (1974). Each sample was adjusted to pH 8.5 with Tris-HCl buffer (50 mM tris [hydroxymethyl] amino-methane + 10 mM EDTA, pH 8.5). Tris 3 ml of HCl buffer (50 mM tris [hydroxymethyl] amino-methane + 10 mM EDTA, pH 8.5] and 0.2 ml of 7.2 mM pyrogallol were added and reacted at 25 ° C for 10 minutes. The reaction was stopped with 1 ml of 1N HCl, , The absorbance at 420 nm was measured and the difference in absorbance between the addition and the absence of addition was expressed as a percentage.

[Equation 1]

SOD-like activity (%) = (1-A / B) X100

In Equation (1), A is the absorbance of the extract-added sphere, and B is the absorbance of the extract-free sphere. However, A and B are values excluding the absorbance of the control.

One of the antioxidant enzymes, superoxide dismutase (SOD), is an enzyme that catalyzes the conversion of superoxide to superoxide. Citrus extract 1 mg and 7.5%, respectively. The prepared mouthwash showed 6.6%, which was 0.95% lower than 1 mg of citrus extract but 1.57% higher than Halabong floral water (see FIG. 2). 2 shows SOD-like activity values of the respective samples.

3) Polyphenol content

Total phenol content was determined by the Folin-Denis method (Folin & Denis 1915). 0.05 ml of the sample was taken in a test tube and distilled water was added thereto to make 0.7 ml. Then, 0.1 ml of Folin-Denis' reagent was added thereto, followed by well mixing and left at room temperature for 3 minutes. After 3 minutes, 0.1 ml of a saturated solution of Na2CO3 was added and mixed. Distilled water was added to make 1 ml, and the mixture was allowed to stand at room temperature for 1 hour. Absorbance of the supernatant was measured at 725 nm. Tannic acid was used as a reference material.

As a result, the content of total phenolic compounds was 37.4mg / g in citrus extract 1mg, 141.4mg in lime 100%, 94.9mg in lime 80%, 25.9mg in haraabong floral water, and 39.4mg in prepared oral cleanser . The lime essential oil is extracted from the fruit skin and contains a relatively large amount of phenol. The prepared mouthwash contains higher phenol than 1 mg of citrus juice and Hanbalong floral water alone.

3 is a graph showing a total polyphenol content value of each sample.

4) Flavonoid content

The flavonoid content of each sample was determined by the method of Moreno (Moreno MIN, Isla MI, Sampietro AR, Vatuone MA (200) Comparison of the free radical scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol, 71, Respectively. In other words, 1 mL of diethylene glycol was added to 100 μL of the sample solution, and the mixture was allowed to stand at room temperature for 5 minutes. Then, 100 μL of 1 N NaOH was added thereto and reacted for 1 hour at 37 ° C. in an incubator. Absorbance was measured at 420 nm using an ELISA reader . Naringin was used as a reference material.

As a result of the experiment, it was found that lime is an essential oil extracted from fruit skin, so it has a flavonoid content of 100mg / g even at 80% dilution. 1 mg of citrus fruits, 17 mg of hanabong floral water, and 9.8 mg of oral cleanser, respectively (see FIG. 4). Fig. 4 shows the flavonoid content of each sample.

2. Analysis of proliferation inhibitory effect on oral bacteria

A single colony of each isolate was isolated and inoculated into 20 ml of growth medium, and cultured for three times at a temperature of 18-24 hours. To prepare the plate culture medium for the antimicrobial activity test, 15 ml of each agar medium was sterilized and 15 ml of each medium was dispensed into petridish to coagulate the growth medium. The medium for medium layer to which 0.7% agar was added was added to the test medium , 0.1 ml of various test bacteria (a suspension of bacteria in which the bacterial suspension was made to have a concentration of 0.4 to 0.5 at a concentration of 650 nm by sterilized saline) was added aseptically and mixed well, and the mixture was dispensed on a heating medium And uniformly solidified to prepare a double-medium inoculated medium. The antimicrobial activity of the samples was measured by the disc plate method. (Piddocket, 1990; Bauer et al., 1996). In other words, each sample was absorbed by 45 μl into sterilized filter paper disc (8 mm) at 20, 40, 80, and 100% concentration, cultured for 24 hours at the appropriate growth temperature of each microorganism, Diameter was measured to compare antimicrobial activity.

As a result of the experiment, it was found that the lime was an essential oil and the antimicrobial effect was excellent and the clear zone was large. All three strains of C. albicans , S. mutans , and S. sobrinus were inhibited by the concentration- And the inhibitory activity of the composition against S. mutans was all in the range of 20 to 100% (FIG. 6). The inhibitory effect of the composition on S. mutans was found to be higher than that of C. albicans and S. sobrinus , and a remarkable result was obtained from 20 to 100% (FIGS. 5 to 7). Therefore, the use of the prepared oral cleanser may be helpful for inhibiting viability of C. albicans causing oral candidiasis, which is commonly referred to as thrush, and S. mutans and S. sobrinus , which are causative agents of dental caries.

1) Antibacterial activity against C. albicans

Figure 5 shows the clear zone size of oral cleanser by concentration for C. albicans .

2) Antimicrobial activity against S. mutans

Figure 6 shows the clear zone size for each concentration of oral detergent against S. mutans .

3) Antibacterial activity against S. sobrinus

Figure 7 shows the clear zone size for each concentration of oral cleanser for S. sobrinus .

3. Survival analysis of oral normal cells (PDL)

Figure 8 shows cell viability for normal oral cells.

In this experiment, 20%, 40%, 80%, and 100% of each sample were mixed with the liquid medium and analyzed by concentration.

In the case of floral water, the lower the concentration, the higher the survival rate. In the case of gagrin (oral cleaning agent as a comparative example), the survival rate was very low at all concentrations, which is a result of the compound components added to the composition of the oral cleaning agent In the case of citrus fruits, the results of the first experiment are not clear, so they are currently being tested and the lime essential oil has a relatively high survival rate in all concentration ranges.

  4. Analysis of inhibitory effect on oral cancer cells (KB)

In order to analyze whether each of the four kinds of samples inhibited the proliferation of KB cells as oral cancer cells, each sample was mixed with a liquid medium to prepare 20%, 40%, 80%, and 100% Respectively.

In the case of floral water, it was found that cancer cells were suppressed very well in all concentration groups. In case of gagrin (mouthwash), most cancer cells were killed at all concentrations similar to floral water. Citrus extract Did not affect cancer cell proliferation at all concentration ranges, and lime essential oil showed relatively high inhibition at all concentration ranges.

9 shows the ability to inhibit proliferation of oral cancer cells.

II. Manufacture of mouthwash

The addition amount of each component can be determined as shown in Table 2 by analyzing the difference in physical properties depending on the amount of the extracts and basic components extracted from citrus or lime.

Raw material name Content (% by weight) Purpose of blending Citrus or lime extract 1.0 to 4.0 Main efficacy component D-sorbitol solution 40 to 60 Wetting agent Vegetable glycerin 2.0 to 3.0 Pollock Sammer 407 2.0 to 10.0 Natural foaming agent (natural surfactant derived from coconut) Citric acid (hydrate) and sodium citrate and sodium citrate 0.005 pH adjusting agent Sodium fluoride (sodium fluoride) and cetylpyridinium chloride and cetylpyridinium chloride
0.22 Caries prevention Mint scent 0.20 Edible flavoring agent Blue No. 1 0.00014 coloring agent Sodium benzoate (sodium benzoate) 0.20 Natural preservative Enzyme treatment Stevia 0.25-0.50 Sweetener Xylitol 0.10 to 0.50 Purified water To 100

A mint scent is any flavoring agent that can impart a mint flavor.

III. Clinical experiment

1. Establishing the condition of composition of functional oral cleanser using extract

According to the composition of the oral cleanser described above, an oral cleanser was prepared as shown in Table 3.

NO Purpose of blending Raw material name Prescription amount (%) Food Additives Remarks One solvent Purified water 83.77 ○ Surfactants Flack Sammer 407 0.1 US Pharmacopeia 2 Wetting agent D-sorbitol 10 ○ (Concentrated) glycerin 5 ○ chief ingredient Sodium fluoride 0.02 Korea Pharmacopeia Efficacy: Cavity prevention Cetylpyridinium chloride 0.05 US Pharmacopeia Efficacy: Removing plaque Sweetener Xylitol 0.1 ○ Enzyme treatment Stevia 0.05 ○ pH adjusting agent Citric acid (hydrate) 0.001 ○ The pH of the solution is adjusted to about 5.5 Sodium citrate 0.3 ○ Preservative Sodium benzoate (sodium benzoate) 0.1 ○ coloring agent Blue No. 1 0.00014 Edible Use food coloring 3 Flavoring agent Mint flavor (for goggles) 0.2 Edible Use an edible flavor Emulsifier Polysorbate 20 0.3 ○ 1.5 times the content of mint flavor 4 extract Citrus extract 0.2 ○ Antibacterial, Antioxidant Lime oil 0.2 ○ Antibacterial, Antioxidant Sum 100

2. Analyzing odor improvement ability of mouthwash composition

In the absence of toothbrushing or any oral supplies, 1 mL of saliva for breathing was placed in the mouth, and breathing of the mouth was opened to collect halitosis in the mouth. Three minutes later, the syringes were pumped 2 ~ 3 times, 1 mL was collected, taken out of the mouth, the 1 mL scale was precisely adjusted, and the air containing the bad breath was pushed in by inserting it into the sensor gas chromatograph ODSA - FIS Inc. The measurement time is 4 minutes, and the results are shown in graphs and tables.

As a result of the experiment, the degree of decrease of the composition before and after the use of hydrogen sulfide (H2S), methyl mercaptan (CH3SH) and methyl disulfide (CH₃) It was found that 94.1% of hydrogen sulfide, 90.9% of methyl mercaptan and 56.1% of methyl disulfide were reduced on average after use of oral cleaning agent than before use (see FIGS. 10 and 11, Table 4).

FIG. 10 shows analysis of changes in the content of halitosis substances in (a) and (b) before use of the oral cleaning agent. Fig. 11 shows analysis of changes in the content of halitosis substances in (a) and (b) before use of the oral cleanser.

Primary Secondary Before use after use Before use after use H2S 29.0 0.0 0.0 1.7 CH3SH 14.4 1.3 0.0 0.0 (CH3) ₂S 24.7 21.7 5.4 0.0

3. Analysis of tooth whitening efficacy of oral cleaning composition

After selecting the specific tooth to be used for the test, the degree of whiteness of the tooth color after using the oral cleansing agent three times a day before use and for 1 month was compared and analyzed by the analyzer (X-rite VS45).

As shown in Table 5, yellowness was decreased by 1.4 or more and whiteness was 1.84 and 5.42, respectively. It is difficult to judge that the improvement is within the range of 1 or less and can not be confirmed by the naked eye. However, it is remarkably improved at the value of 1 or more. Therefore, the effect of tooth whitening was observed in both of the subjects. The effectiveness of tooth whitening can be expected by using the manufactured oral cleanser. Table 5 shows tooth color measurements before and after use of the oral cleanser. In Table 5, YI-E313: yellowness value, WI-CIE is whiteness value, and L *, DL *: brightness.

Name YI-E313 WI-CIE L * DL * k group 37.15 -32.71 78.28  -1.52 D k group 35.67 -30.87 77.17  -2.63 D j sheep 63.14 -108.23 36.24 -15.53 D j sheep 61.74 -102.81 39.01 -12.76 D

Finally, the preference and sensory evaluation of the prototype showed that the color, flavor, and taste of the mouthwash were generally good, and the mouthwash after the goggle was clean / clean, the bad breath was improved, the refreshingness was good and the mouthwash .

According to the present invention, the oral cleaning agent has antioxidative activity, inhibition and sterilization of oral noxious bacteria, and improvement of dental calculus as major effects.

Also, according to the present invention, the oral cleanser has good color, flavor, and taste, and is lukewarm / clean after gargle, has improved bad breath and has a refreshing effect upon use.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (6)

delete delete delete Citrus or lime in a tank net of an essential oil extracting apparatus to extract essential oils at 110 ° C for 8 hours while separating the floral water;
Adding 1 to 4% by weight of the extract extracted from citrus or lime (the essential oil or the floral water) to the solvent; And
(Hydrous) and sodium citrate in an amount of 0.005% by weight, sodium fluoride (sodium fluoride) and cetylpyridinium chloride (sodium fluoride) in an amount of 40 to 60% by weight, D-sorbitol solution in an amount of 40 to 60% by weight, vegetable glycerin in an amount of 2.0 to 3.0% by weight, Pollockamer 407 in an amount of 2.0 to 10.0% 0.22 wt.%, Mint scent 0.20 wt.%, Blue No. 1 0.00014 wt.%, Sodium benzoate (sodium benzoate) 0.20 wt.%, Enzyme treated stevia 0.25-0.50 wt.%, Xylitol 0.10-0.50 wt. To the solvent. ≪ RTI ID = 0.0 > 11. < / RTI > 5. The method of claim 4,
Wherein the citrus fruit is Halabon. 5. The method of claim 4,
Wherein the citrus extract or the extract extracted from the lime is essential oil or Floral Water.

Images