CN109042268A - A kind of implantation methods improving potato nutritive value - Google Patents
A kind of implantation methods improving potato nutritive value Download PDFInfo
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- CN109042268A CN109042268A CN201810860950.XA CN201810860950A CN109042268A CN 109042268 A CN109042268 A CN 109042268A CN 201810860950 A CN201810860950 A CN 201810860950A CN 109042268 A CN109042268 A CN 109042268A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses a kind of implantation methods for improving potato nutritive value, belong to potato planting technology field, comprising the following steps: (1) preparation of matrix；(2) potato is handled；(3) it is inoculated with；(4) it transplants.The potato of implantation methods plantation through the invention, nutrition content is abundant, high to the biological transformation ratio of nutrient, and robust growth, yield is high, and potato quality is good, and base starting material is cheap, at low cost, great market popularization value.
Description
Technical field
The invention belongs to potato planting technology fields, and in particular to a kind of implantation methods for improving potato nutritive value.
Background technique
Potato has very high nutritive value and medical value, and nutrient is abundant, contains protein, minerals, dimension life
The Multiple components such as element, trophic structure also relatively rationally, there is the title of " underground apple ".Protein and vitamin c, vitamin contained by it
B1, vitamin B2 are more much higher than apple: vitamin C is 10 times of apple.B family vitamin is 4 times of apple.Calcium, phosphorus, magnesium, potassium
Content is that several times to tens times of apple differ, especially the content of potassium, it may be said that ranked first position in greengrocery.Rich in a large amount of
Carbohydrate can supply a large amount of thermal energy of human body.The skin of potato is rich in chlorogenic acid and lipoic acid.Chlorogenic acid has anti-oxidant and anti-
The effect of cancer, lipoic acid Plantain prevent skin aging.Containing the dietary antioxidants that can help to prevent infirmities of age and
There is a large amount of good fiber quality element, there is the effects of prevent constipation and anti-curing cancers.
The plantation of existing potato, by stem tuber come breed be most directly and it is quick in a manner of, but often because of planting matrix
There is no specific aim, leads to the quality of potato worse and worse.Existing potato vegetative propagation planting matrix matter is all addition a large amount ofization
Object, such as calcium chloride, potassium nitrate, manganese sulfate are closed, cost is very high, although can be very good to promote the differentiation of stem tuber to germinate,
It is the diseases and insect pests resistance that can not improve potato.
Summary of the invention
The purpose of the present invention is being directed to existing problem, a kind of implantation methods for improving potato nutritive value are provided.It is logical
The potato of implantation methods plantation of the invention is crossed, nutrition content is abundant, and high to the biological transformation ratio of nutrient, growth is strong
Strong, yield is high, and potato quality is good, and base starting material is cheap, at low cost, great market popularization value.
The present invention is achieved by the following technical solutions:
A kind of implantation methods improving potato nutritive value, comprising the following steps:
(1) preparation of matrix:
A. 22 ~ 28 parts of 42 ~ 48 parts of modified corn core, banana skin, the waxgourd peel 13 ~ 17 of corresponding parts by weight Freezing smashing: are weighed
Part, 14 ~ 18 parts of pumpkin peel, 11 ~ 15 parts of wheat bran, 13 ~ 17 parts of mangosteen shell, 14 ~ 16 parts of watermelon peel, 14.5 ~ 15.5 parts of pomelo peel, ferment
11 ~ 15 parts of female selenium is collectively disposed in the deep cooling type pulverizer of pre-cooling and carries out pulverization process, smashes it through 20 meshes；
B. anti-oxidant treatment: will operate the powder after being sieved in a and purified water according to weight ratio is that 1:16 ~ 19 are collectively disposed at instead
Answer in kettle, then weigh sieving after powder total weight parts 0.22 ~ 0.28% 4- hexyl resorcin, 0.26 ~ 0.34% it is anti-bad
Hematic acid palmitate, 0.32 ~ 0.38% sorbic acid and its sylvite, 0.62 ~ 0.66% ethephon (CEPHA),2-(chloroethyl) phosphonic acid, 0.22 ~ 0.28% brassin
It is collectively disposed in reaction kettle, mixture is obtained after 26 ~ 34min of stir process；
C. radiation treatment: will operate the mixture obtained in b and be placed in irradiation instrument and carry out radiation treatment, the mode of radiation treatment
It is β ray, the time of radiation treatment is 2.2 ~ 2.8h, and the dosage of radiation treatment is 2.6 ~ 3.4Gy/min；
D. prepared by matrix: will operate mixture in c after radiation treatment and agar according to weight ratio is that 1:0.32 ~ 0.38 is common
It is added in agitator tank, then measures 7.5 ~ 8.5 times of total weight of the mixture part of sterile water and be placed in agitator tank, be kept stirring tank
Interior temperature is 100 ~ 102 DEG C, 47 ~ 53min of stir process, takes out mixture and is cooled to room temperature；
(2) potato is handled: the potato decortication of fresh and healthy is placed in bactericidal liquid and carries out immersion treatment, time of immersion is 15 ~
19min；
(3) it is inoculated with: the potato in step (2) is cut into 2 ~ 2.5cm3Small cube, be inoculated into step (1) preparation matrix in,
Matrix is set in the light incubator, daily that negative aeroion is periodically passed through into illumination box, the time being passed through daily is
Afternoon 15:00 ~ 16:00, each intake control is 0.07 ~ 0.09ppm；
(4) it transplants: the potato sprout in step (3), when seedling height grows to 6 ~ 8cm, by potato transplantation of seedlings to planting matrix
Ground.
Further, the preparation method of modified corn core in a is operated in the step (1), comprising the following steps:
It 1) is that 1:2.7 ~ 3.3 are added to jointly in microjet high pressure homogenizer according to weight ratio by corncob and modification liquid, homogeneous
The operating pressure control of machine is 125 ~ 145MPa, and the time of high pressure microjet processing is 13 ~ 15min；
2) corncob operated in 1) taking-up is placed in Buchner funnel and is filtered, filter residue is dried, it is dry to constant weight
, dry temperature control is 61 ~ 65 DEG C.
Further, the modification liquid is made of following parts by weight of component: 6.5 ~ 7.5 parts of nanometer iron powder, attapulgite
3.1 ~ 3.5 parts, 1.5 ~ 2.5 parts of polysorbas20,2.4 ~ 2.6 parts of praseodymium acetate, 1.5 ~ 1.7 parts of methyl α-naphthyl acetate, 125 ~ 155 parts of deionized water.
Further, the revolving speed of pulverizer is 1250 ~ 1350rpm in operation a in the step (1), pulverization process when
Between be 32 ~ 38min,
Further, the temperature of deep cooling type pulverizer pre-cooling is -19 ~ -17 DEG C, in crushing process in operation a in the step (1)
Temperature control in pulverizer is -7 ~ -5 DEG C.
Further, the temperature control in the step (1) in operation b in reaction kettle is 106 ~ 108 DEG C, in reaction kettle
Pressure control is 0.35 ~ 0.45MPa.
Further, bactericidal liquid in the step (2), is made of: 2 ~ 4 parts of fulvic acid, 75% ethyl alcohol following parts by weight of component
70 ~ 80 parts, 1.3 ~ 1.9 parts of sodium hypochlorite, 0.5 ~ 0.7 part of lysozyme, 10 ~ 18 parts of Aloe Vera Gel.
Further, the temperature control in the step (2) in bactericidal liquid is 35 ~ 39 DEG C.
The present invention has carried out very big optimization on the basis of existing potato implantation methods, is to planting matrix first
Modified corn core, banana skin, waxgourd peel, pumpkin peel etc. are carried out Freezing smashing first in the process for preparation of matrix by optimization,
Freezing smashing can guarantee the chemical stability of effective component in raw material to greatest extent, moreover it is possible to inhibit microorganism and enzyme in raw material
Activity, prevent raw material denaturation, rotten, after crushing, contact with each other between effective component area increase in raw material.In addition
In raw material, applicant is added after being modified corncob, in the modifying process of corncob, by corncob with change
Property liquid be added in microjet high pressure homogenizer, under the action of high pressure microjet, ingredient in corncob and modification liquid is carried out
The effects of high velocity impact, the higher-order of oscillation, instantaneous pressure drop and intensive shear, cavitation, viscosity reduce, the carbon hydrate of corncob
Very big change all occurs for object structure and function activity.Modified corn core is combined closely after crushing with yeast selenium, is enhanced
The activity of yeast selenium improves the bioavilability of selenium element, promotes the nutritive value of potato.It and then will be between raw material and 4- hexyl
Benzenediol, ascorbyl palmitate, sorbic acid and its sylvite, ethephon (CEPHA),2-(chloroethyl) phosphonic acid, brassin carry out hair under high-temperature and high-pressure conditions and answer,
On the one hand it is to cure raw material, effective component is made to be converted to the structure type for being easier to be absorbed and utilized by potato, 4- hexylresorcinol two
Phenol, ascorbyl palmitate, sorbic acid and its sylvite have anti-oxidant, corrosion-resistant effect, because organic additive is exposed to
It is easily aoxidized by the oxygen in air in air, the quality of matrix can have a greatly reduced quality, by the effect of antioxidant, very well
The chemical stability for protecting effective component in raw material, ethephon (CEPHA),2-(chloroethyl) phosphonic acid, brassin are not only plant growth regulator, promote plant
Growth and development, also reply plant to extraneous biology or abiotic stress, improve the diseases and insect pests resistance of potato.Raw material is mixed again
It closes object and carries out β ray, stimulate the expression of a variety of enzyme genes, promote the generation and enrichment of nutritional ingredient substance in potato.Matrix system
After the completion of standby, potato is handled, potato decortication is placed in bactericidal liquid impregnates first, primarily to removal potato
The germ on surface and worm's ovum operate for subsequent inoculation and establish excellent basis, are then inoculated into potato stripping and slicing and to prepare
In matrix, during culture, it is periodically passed through negative aeroion daily, has very to the respiration and photosynthesis of plant
Good facilitation, and can also Absorption And Metabolism pollutant, provide good environment for the growth and development of plant.
The present invention has the advantage that compared with prior art
The potato of implantation methods plantation through the invention, nutrition content is abundant, high to the biological transformation ratio of nutrient,
Robust growth, yield is high, and potato quality is good, and base starting material is cheap, at low cost, great market popularization value.
Specific embodiment
Embodiment 1
A kind of implantation methods improving potato nutritive value, comprising the following steps:
(1) preparation of matrix:
A. 13 parts of 22 parts of 42 parts of modified corn core, banana skin, waxgourd peel, the pumpkin peel 14 of corresponding parts by weight Freezing smashing: are weighed
Part, 11 parts of wheat bran, 13 parts of mangosteen shell, 14 parts of watermelon peel, 14.5 parts of pomelo peel, 11 parts of the yeast selenium deep cooling types for being collectively disposed at pre-cooling
Pulverization process is carried out in pulverizer, smashes it through 20 meshes；
B. anti-oxidant treatment: will operate the powder after being sieved in a and purified water according to weight ratio is that 1:16 is collectively disposed at reaction kettle
In, then weigh sieving after powder total weight parts 0.22% 4- hexyl resorcin, 0.26% ascorbyl palmitate,
0.32% sorbic acid and its sylvite, 0.62% ethephon (CEPHA),2-(chloroethyl) phosphonic acid, 0.22% brassin be collectively disposed in reaction kettle, stir process
Mixture is obtained after 26min；
C. radiation treatment: will operate the mixture obtained in b and be placed in irradiation instrument and carry out radiation treatment, the mode of radiation treatment
It is β ray, the time of radiation treatment is 2.2h, and the dosage of radiation treatment is 2.6Gy/min；
D. prepared by matrix: will operate mixture in c after radiation treatment and agar according to weight ratio is that 1:0.32 is added to jointly
In agitator tank, then measures 7.5 times of total weight of the mixture part of sterile water and be placed in agitator tank, the temperature being kept stirring in tank is
It 100 DEG C, stir process 47min, takes out mixture and is cooled to room temperature；
(2) potato is handled: the potato decortication of fresh and healthy being placed in bactericidal liquid and carries out immersion treatment, the time of immersion is
15min；
(3) it is inoculated with: the potato in step (2) is cut into 2cm3Small cube, be inoculated into step (1) preparation matrix in, by base
Matter is set in the light incubator, and timing is passed through negative aeroion into illumination box daily, and the time being passed through daily is afternoon
15:00, each intake control is 0.07ppm；
(4) it transplants: the potato sprout in step (3), when seedling height grows to 6cm, by potato transplantation of seedlings to planting base
?.
Further, the preparation method of modified corn core in a is operated in the step (1), comprising the following steps:
It 1) is that 1:2.7 is added to jointly in microjet high pressure homogenizer according to weight ratio by corncob and modification liquid, homogenizer
Operating pressure control is 125MPa, and the time of high pressure microjet processing is 13min；
2) corncob operated in 1) taking-up is placed in Buchner funnel and is filtered, filter residue is dried, it is dry to constant weight
, dry temperature control is 61 DEG C.
Further, the modification liquid is made of following parts by weight of component: 6.5 parts of nanometer iron powder, 3.1 parts of attapulgite,
1.5 parts of polysorbas20,2.4 parts of praseodymium acetate, 1.5 parts of methyl α-naphthyl acetate, 125 parts of deionized water.
Further, the revolving speed of pulverizer is 1250rpm in operation a in the step (1), and the time of pulverization process is
32min,
Further, the temperature of deep cooling type pulverizer pre-cooling is -19 DEG C in operation a in the step (1), is crushed in crushing process
Temperature control in machine is -7 DEG C.
Further, the temperature control in the step (1) in operation b in reaction kettle is 106 DEG C, the pressure in reaction kettle
Control is 0.35MPa.
Further, bactericidal liquid in the step (2), is made of following parts by weight of component: 2 parts of fulvic acid, 75% ethyl alcohol 70
Part, 1.3 parts of sodium hypochlorite, 0.5 part of lysozyme, 10 parts of Aloe Vera Gel.
Further, the temperature control in the step (2) in bactericidal liquid is 35 DEG C.
Embodiment 2
A kind of implantation methods improving potato nutritive value, comprising the following steps:
(1) preparation of matrix:
A. 15 parts of 25 parts of 45 parts of modified corn core, banana skin, waxgourd peel, the pumpkin peel 16 of corresponding parts by weight Freezing smashing: are weighed
Part, 13 parts of wheat bran, 15 parts of mangosteen shell, 15 parts of watermelon peel, 15 parts of pomelo peel, 13 parts of the yeast selenium deep cooling type powder for being collectively disposed at pre-cooling
Pulverization process is carried out in broken machine, smashes it through 20 meshes；
B. anti-oxidant treatment: will operate the powder after being sieved in a and purified water according to weight ratio is that 1:17 is collectively disposed at reaction kettle
In, then weigh sieving after powder total weight parts 0.25% 4- hexyl resorcin, 0.3% ascorbyl palmitate,
0.35% sorbic acid and its sylvite, 0.64% ethephon (CEPHA),2-(chloroethyl) phosphonic acid, 0.25% brassin be collectively disposed in reaction kettle, stir process
Mixture is obtained after 30min；
C. radiation treatment: will operate the mixture obtained in b and be placed in irradiation instrument and carry out radiation treatment, the mode of radiation treatment
It is β ray, the time of radiation treatment is 2.5h, and the dosage of radiation treatment is 3Gy/min；
D. prepared by matrix: will operate mixture in c after radiation treatment and agar according to weight ratio is that 1:0.35 is added to jointly
In agitator tank, then measures 8 times of total weight of the mixture part of sterile water and be placed in agitator tank, the temperature being kept stirring in tank is
It 101 DEG C, stir process 50min, takes out mixture and is cooled to room temperature；
(2) potato is handled: the potato decortication of fresh and healthy being placed in bactericidal liquid and carries out immersion treatment, the time of immersion is
17min；
(3) it is inoculated with: the potato in step (2) is cut into 2.25cm3Small cube, be inoculated into step (1) preparation matrix in, will
Matrix is set in the light incubator, and timing is passed through negative aeroion into illumination box daily, under the time being passed through daily is
Noon 15:30, each intake control is 0.08ppm；
(4) it transplants: the potato sprout in step (3), when seedling height grows to 7cm, by potato transplantation of seedlings to planting base
?.
Further, the preparation method of modified corn core in a is operated in the step (1), comprising the following steps:
It 1) is that 1:3 is added to jointly in microjet high pressure homogenizer according to weight ratio by corncob and modification liquid, the work of homogenizer
Making pressure control is 135MPa, and the time of high pressure microjet processing is 14min；
2) corncob operated in 1) taking-up is placed in Buchner funnel and is filtered, filter residue is dried, it is dry to constant weight
, dry temperature control is 63 DEG C.
Further, the modification liquid is made of following parts by weight of component: 7 parts of nanometer iron powder, is spat 3.3 parts of attapulgite
Warm 20 2 parts, 2.5 parts of praseodymium acetate, 1.6 parts of methyl α-naphthyl acetate, 140 parts of deionized water.
Further, the revolving speed of pulverizer is 1300rpm in operation a in the step (1), and the time of pulverization process is
35min,
Further, the temperature of deep cooling type pulverizer pre-cooling is -18 DEG C in operation a in the step (1), is crushed in crushing process
Temperature control in machine is -6 DEG C.
Further, the temperature control in the step (1) in operation b in reaction kettle is 107 DEG C, the pressure in reaction kettle
Control is 0.4MPa.
Further, bactericidal liquid in the step (2), is made of following parts by weight of component: 3 parts of fulvic acid, 75% ethyl alcohol 75
Part, 1.6 parts of sodium hypochlorite, 0.6 part of lysozyme, 14 parts of Aloe Vera Gel.
Further, the temperature control in the step (2) in bactericidal liquid is 37 DEG C.
Embodiment 3
A kind of implantation methods improving potato nutritive value, comprising the following steps:
(1) preparation of matrix:
A. 17 parts of 28 parts of 48 parts of modified corn core, banana skin, waxgourd peel, the pumpkin peel 18 of corresponding parts by weight Freezing smashing: are weighed
Part, 15 parts of wheat bran, 17 parts of mangosteen shell, 16 parts of watermelon peel, 15.5 parts of pomelo peel, 15 parts of the yeast selenium deep cooling types for being collectively disposed at pre-cooling
Pulverization process is carried out in pulverizer, smashes it through 20 meshes；
B. anti-oxidant treatment: will operate the powder after being sieved in a and purified water according to weight ratio is that 1:19 is collectively disposed at reaction
In kettle, then weigh sieving after powder total weight parts 0.28% 4- hexyl resorcin, 0.34% ascorbyl palmitate,
0.38% sorbic acid and its sylvite, 0.66% ethephon (CEPHA),2-(chloroethyl) phosphonic acid, 0.28% brassin be collectively disposed in reaction kettle, stir process
Mixture is obtained after 34min；
C. radiation treatment: will operate the mixture obtained in b and be placed in irradiation instrument and carry out radiation treatment, the mode of radiation treatment
It is β ray, the time of radiation treatment is 2.8h, and the dosage of radiation treatment is 3.4Gy/min；
D. prepared by matrix: will operate mixture in c after radiation treatment and agar according to weight ratio is that 1:0.38 is added to jointly
In agitator tank, then measures 8.5 times of total weight of the mixture part of sterile water and be placed in agitator tank, the temperature being kept stirring in tank is
It 102 DEG C, stir process 53min, takes out mixture and is cooled to room temperature；
(2) potato is handled: the potato decortication of fresh and healthy being placed in bactericidal liquid and carries out immersion treatment, the time of immersion is
19min；
(3) it is inoculated with: the potato in step (2) is cut into 2.5cm3Small cube, be inoculated into step (1) preparation matrix in, will
Matrix is set in the light incubator, and timing is passed through negative aeroion into illumination box daily, under the time being passed through daily is
Noon 16:00, each intake control is 0.09ppm；
(4) it transplants: the potato sprout in step (3), when seedling height grows to 8cm, by potato transplantation of seedlings to planting base
?.
Further, the preparation method of modified corn core in a is operated in the step (1), comprising the following steps:
It 1) is that 1:3.3 is added to jointly in microjet high pressure homogenizer according to weight ratio by corncob and modification liquid, homogenizer
Operating pressure control be 145MPa, high pressure microjet processing time be 15min；
2) corncob operated in 1) taking-up is placed in Buchner funnel and is filtered, filter residue is dried, it is dry to constant weight
, dry temperature control is 65 DEG C.
Further, the modification liquid is made of following parts by weight of component: 7.5 parts of nanometer iron powder, 3.5 parts of attapulgite,
2.5 parts of polysorbas20,2.6 parts of praseodymium acetate, 1.7 parts of methyl α-naphthyl acetate, 155 parts of deionized water.
Further, the revolving speed of pulverizer is 1350rpm in operation a in the step (1), and the time of pulverization process is
38min,
Further, the temperature of deep cooling type pulverizer pre-cooling is -17 DEG C in operation a in the step (1), is crushed in crushing process
Temperature control in machine is -5 DEG C.
Further, the temperature control in the step (1) in operation b in reaction kettle is 108 DEG C, the pressure in reaction kettle
Control is 0.45MPa.
Further, bactericidal liquid in the step (2), is made of following parts by weight of component: 4 parts of fulvic acid, 75% ethyl alcohol 80
Part, 1.9 parts of sodium hypochlorite, 0.7 part of lysozyme, 18 parts of Aloe Vera Gel.
Further, the temperature control in the step (2) in bactericidal liquid is 39 DEG C.
Comparative example 1
This comparative example 1 compared with Example 2, changes into the modified corn core in a is operated in the preparation of step (1) matrix
The non-modified corncob of mass parts, method and step in addition to this are all the same.
Comparative example 2
This comparative example 2 compared with Example 2, is saved and operates yeast selenium in a in the preparation of step (1) matrix, in addition to this
Method and step is all the same.
Comparative example 3
This comparative example 3 compared with Example 2, is saved the whole operation process for operating b in the preparation of step (1) matrix, is removed
Furthermore method and step is all the same.
Comparative example 4
This comparative example 4 compared with Example 2, is saved the whole operation process for operating c in the preparation of step (1) matrix, is removed
Furthermore method and step is all the same.
Comparative example 5
This comparative example 5 compared with Example 2, saves the whole operation process of step (2) potato processing, method in addition to this
Step is all the same.
Comparative example 6
This comparative example 6 compared with Example 2, saves the negative aeroion in step (3) inoculation, method and step in addition to this
It is all the same.
Control group
Existing potato stem tuber implantation methods.
In order to compare effect of the present invention, same a collection of potato is harvested from the same planting base, therefrom picking size
Almost completely the same potato is as experimental subjects, respectively with embodiment 2, comparative example 1, comparative example 2, comparison
Embodiment 3, comparative example 4, comparative example 5, comparative example 6, control group implantation methods planted, wait until potato
After maturation, potato is harvested, the Se content of every group of potato is measured；
From Wuhan University's animal experimental center purchase 80, the SPF grade mouse at constitution size intimate 2 completely the same monthly ages,
SPF grades of animal houses adapt to after feeding 7 days, they are randomly divided into 8 groups, then every group of 10 mouse use above-described embodiment respectively
2, comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6, right
Stomach-filling is distinguished to every group of mouse according to the potato of group, the stomach-filling time is every morning 9:00-9:30, continues stomach-filling 12 weeks, experiment periods
Between the free diet drinking-water of all mouse, measure the intracorporal Se content of mouse.
Specific experiment correlation data is as shown in table 1 below:
Table 1
| Potato Se content (mg/kg) | Mouse Se content (μ g/g) | |
| Embodiment 2 | 0.35 | 0.26 |
| Comparative example 1 | 0.29 | 0.21 |
| Comparative example 2 | 0.08 | 0.09 |
| Comparative example 3 | 0.33 | 0.23 |
| Comparative example 4 | 0.32 | 0.20 |
| Comparative example 5 | 0.34 | 0.25 |
| Comparative example 6 | 0.32 | 0.24 |
| Control group | 0.07 | 0.08 |
The potato of implantation methods plantation it can be seen from upper table 1 through the invention, nutrition content is abundant, to nutrition member
The biological transformation ratio of element is high, robust growth, and yield is high, and potato quality is good, and base starting material is cheap, at low cost, great
Market popularization value.
Claims (8)
1. a kind of implantation methods for improving potato nutritive value, which comprises the following steps:
(1) preparation of matrix:
A. 22 ~ 28 parts of 42 ~ 48 parts of modified corn core, banana skin, the waxgourd peel 13 ~ 17 of corresponding parts by weight Freezing smashing: are weighed
Part, 14 ~ 18 parts of pumpkin peel, 11 ~ 15 parts of wheat bran, 13 ~ 17 parts of mangosteen shell, 14 ~ 16 parts of watermelon peel, 14.5 ~ 15.5 parts of pomelo peel, ferment
11 ~ 15 parts of female selenium is collectively disposed in the deep cooling type pulverizer of pre-cooling and carries out pulverization process, smashes it through 20 meshes；
B. anti-oxidant treatment: will operate the powder after being sieved in a and purified water according to weight ratio is that 1:16 ~ 19 are collectively disposed at instead
Answer in kettle, then weigh sieving after powder total weight parts 0.22 ~ 0.28% 4- hexyl resorcin, 0.26 ~ 0.34% it is anti-bad
Hematic acid palmitate, 0.32 ~ 0.38% sorbic acid and its sylvite, 0.62 ~ 0.66% ethephon (CEPHA),2-(chloroethyl) phosphonic acid, 0.22 ~ 0.28% brassin
It is collectively disposed in reaction kettle, mixture is obtained after 26 ~ 34min of stir process；
C. radiation treatment: will operate the mixture obtained in b and be placed in irradiation instrument and carry out radiation treatment, the mode of radiation treatment
It is β ray, the time of radiation treatment is 2.2 ~ 2.8h, and the dosage of radiation treatment is 2.6 ~ 3.4Gy/min；
D. prepared by matrix: will operate mixture in c after radiation treatment and agar according to weight ratio is that 1:0.32 ~ 0.38 is common
It is added in agitator tank, then measures 7.5 ~ 8.5 times of total weight of the mixture part of sterile water and be placed in agitator tank, be kept stirring tank
Interior temperature is 100 ~ 102 DEG C, 47 ~ 53min of stir process, takes out mixture and is cooled to room temperature；
(2) potato is handled: the potato decortication of fresh and healthy is placed in bactericidal liquid and carries out immersion treatment, time of immersion is 15 ~
19min；
(3) it is inoculated with: the potato in step (2) is cut into 2 ~ 2.5cm3Small cube, be inoculated into step (1) preparation matrix in, will
Matrix is set in the light incubator, and timing is passed through negative aeroion into illumination box daily, under the time being passed through daily is
Noon 15:00 ~ 16:00, each intake control is 0.07 ~ 0.09ppm；
(4) it transplants: the potato sprout in step (3), when seedling height grows to 6 ~ 8cm, by potato transplantation of seedlings to planting matrix
Ground.
2. a kind of implantation methods for improving potato nutritive value according to claim 1, which is characterized in that the step (1)
The preparation method of modified corn core in middle operation a, comprising the following steps:
It 1) is that 1:2.7 ~ 3.3 are added to jointly in microjet high pressure homogenizer according to weight ratio by corncob and modification liquid, homogeneous
The operating pressure control of machine is 125 ~ 145MPa, and the time of high pressure microjet processing is 13 ~ 15min；
2) corncob operated in 1) taking-up is placed in Buchner funnel and is filtered, filter residue is dried, it is dry to constant weight
, dry temperature control is 61 ~ 65 DEG C.
3. a kind of implantation methods for improving potato nutritive value according to claim 2, which is characterized in that the modification liquid,
It is made of following parts by weight of component: 6.5 ~ 7.5 parts of nanometer iron powder, 3.1 ~ 3.5 parts of attapulgite, 1.5 ~ 2.5 parts of polysorbas20, vinegar
2.4 ~ 2.6 parts of sour praseodymium, 1.5 ~ 1.7 parts of methyl α-naphthyl acetate, 125 ~ 155 parts of deionized water.
4. a kind of implantation methods for improving potato nutritive value according to claim 1, which is characterized in that the step (1)
The revolving speed of pulverizer is 1250 ~ 1350rpm in middle operation a, and the time of pulverization process is 32 ~ 38min.
5. a kind of implantation methods for improving potato nutritive value according to claim 1, which is characterized in that the step (1)
The temperature of deep cooling type pulverizer pre-cooling is -19 ~ -17 DEG C in middle operation a, the control of temperature in crushing process in pulverizer for -7 ~ -
5℃。
6. a kind of implantation methods for improving potato nutritive value according to claim 1, which is characterized in that the step (1)
Temperature control in middle operation b in reaction kettle is 106 ~ 108 DEG C, and the pressure control in reaction kettle is 0.35 ~ 0.45MPa.
7. a kind of implantation methods for improving potato nutritive value according to claim 1, which is characterized in that the step (2)
Middle bactericidal liquid is made of following parts by weight of component: 2 ~ 4 parts of fulvic acid, 70 ~ 80 parts of 75% ethyl alcohol, 1.3 ~ 1.9 parts of sodium hypochlorite, molten
0.5 ~ 0.7 part of bacterium enzyme, 10 ~ 18 parts of Aloe Vera Gel.
8. a kind of implantation methods for improving potato nutritive value according to claim 1, which is characterized in that the step (2)
Temperature control in middle bactericidal liquid is 35 ~ 39 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122239A (en) * | 2019-06-14 | 2019-08-16 | 毕节市农业科学研究所 | A method of enhancing potato plant drought tolerance |
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2018
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122239A (en) * | 2019-06-14 | 2019-08-16 | 毕节市农业科学研究所 | A method of enhancing potato plant drought tolerance |
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