CN109042136A - A kind of breeding method of tara vine - Google Patents
A kind of breeding method of tara vine Download PDFInfo
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- CN109042136A CN109042136A CN201810956319.XA CN201810956319A CN109042136A CN 109042136 A CN109042136 A CN 109042136A CN 201810956319 A CN201810956319 A CN 201810956319A CN 109042136 A CN109042136 A CN 109042136A
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- tara vine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of breeding methods of tara vine, comprising the following steps: chooses the stem apex of tara vine as explant, carry out disinfection to it inoculation；It is gone within 25-35 days after disinfection inoculation and carries out expanding numerous culture in subculture medium；Expanding after numerous culture 35-45 days will be in the tissue culture transplantation of seedlings to sand bed that taken root；Transplanting is into nutritive cube after the tissue-cultured seedling in sand bed is grown 40-50 days；The outdoor cropping when the tissue-cultured seedling in nutritive cube grows to 30-40cm.Its tree body growing way of the invention is strong, and branches and tendrils are sturdy, and winter resistance is strong, and winter occurs without freeze injury, cold-proof without undercarriage, easily formation bud, and flower amount is big, and the florescence is long, realizes efficient productive target.
Description
Technical field
The present invention relates to Kiwi berrys to cultivate field, the breeding method of specifically a kind of tara vine.
Background technique
Tara vine is Actinidiaceae, Actinidia large size defoliation liana；Sprig is substantially hairless or young tender clock star
Dissipate ground thin quilt softness villus or fine hair, leaf film quality or papery, oval, oblong, wealthy oval to subcircular, the anxious short point in top, base
For portion's circle to shallow heart, back side green, inflorescence axillary or armpit are external, and bract is linear, spend green white or yellow green, fragrance, sepal
Oval is to oblong, and the wedge-shaped obovate of petal or wooden dipper shape fall wealthy oval, and filigree is Filamentous, anther black or mulberry, oblong
Arrow shaped, fruit spheroidal to column oblong is 2-3 centimetres long, green-yellow or aubergine when mature, and seed indulges about 2.5 millimeters of diameter.
Tara vine make it is medicinal, be strong, antipyretic and astringent；It is the very high food of nutritive value again.Fruit can both give birth to
Food, can also jam processed, preserved fruit, can, wine brewing etc..Flower is nectar source, can also mention aromatic oil.This kind can not only be used for ornamental tree species, again
It can be used as fruit tree.Therefore, for the above status, there is an urgent need to develop a kind of breeding methods of tara vine, current to overcome
Deficiency in practical application.
Summary of the invention
The purpose of the present invention is to provide a kind of breeding methods of tara vine, to solve to propose in above-mentioned background technique
The problem of.
To achieve the above object, the invention provides the following technical scheme:
A kind of breeding method of tara vine, comprising the following steps:
S1 chooses the stem apex of tara vine as explant, and carry out disinfection to it inoculation；
S2 goes to after disinfection inoculation and carries out expanding numerous culture in subculture medium for 25-35 days；
S3, expanding after numerous culture 35-45 days will be in the tissue culture transplantation of seedlings to sand bed that taken root；
S4, transplanting is into nutritive cube after the tissue-cultured seedling in sand bed is grown 40-50 days；
S5, the outdoor cropping when the tissue-cultured seedling in nutritive cube grows to 30-40cm.
As a further solution of the present invention: in step s 2, going in subculture medium within 30 days and carry out after disinfection inoculation
Expand numerous culture.
As a further solution of the present invention: in step s3, expanding after numerous culture 40 days for the tissue culture transplantation of seedlings taken root extremely
In sand bed.
As a further solution of the present invention: in step s 4, transplanting is extremely sought after the tissue-cultured seedling in sand bed is grown 45 days
It supports in alms bowl.
As a further solution of the present invention: in step s 5, the open country when the tissue-cultured seedling in nutritive cube grows to 35cm
Cultivation.
Compared with prior art, the beneficial effects of the present invention are: the breeding method of the tara vine, tree body growing way
By force, branches and tendrils are sturdy, and winter resistance is strong, and winter occurs without freeze injury, cold-proof without undercarriage, easily formation bud, and flower amount is big, and the florescence is long, in fact
Now efficient productive target.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
A kind of breeding method of tara vine, comprising the following steps:
S1 chooses the stem apex of tara vine as explant, and carry out disinfection to it inoculation；
S2 goes to after disinfection inoculation and carries out expanding numerous culture in subculture medium for 25 days；
S3, expanding after numerous culture 35 days will be in the tissue culture transplantation of seedlings to sand bed that taken root；
S4, transplanting is into nutritive cube after the tissue-cultured seedling in sand bed is grown 40 days；
S5, the outdoor cropping when the tissue-cultured seedling in nutritive cube grows to 30cm.
Tara vine fruit elongated cylindrical, emerald green, peel smooth is hairless, and skin depth 0.09cm, wax layer is obvious, fruit
Background color is yellow, no fruit dot.Stalk is hollow wide shallow, and stem has cyclic annular moth patch, the long 1.68-1.71cm of carpopodium.Calyx low-lying area harbors sub-wire shape column
Head, sepal fall off.Pulp green.Core oblong, white indulge diameter 3.18cm, transverse diameter 1.24cm.Each fruit seed average out to
120, seed brown, ellipse, mass of 1000 kernel 2.26g.Fruit is big, and fresh fruit indulges diameter 4.43cm, transverse diameter 2.91cm, side diameter
2.27cm.Mean Fruit Weight 16.32g, the heavy 23.7g of maximum fruit, nutrient constituents of fruit are as shown in table 1 below:
| Nutritional ingredient | Unit | Content |
| Vitamin C | mg/100g | 359 |
| Vitamin B2 | mg/100g | 0.027 |
| Vitamin B6 | mg/100g | 1.4 |
| Soluble solid | g/100g | 16.0 |
| Titratable acid | g/100g | 1.19 |
| Total reducing sugar | g/100g | 8.3 |
| Tartaric acid | mg/100g | 0.96 |
| Citric acid | mg/100g | 20.26 |
| Asparatate ASP | mg/100g | 139 |
| Glutamic acid GLU | mg/100g | 328 |
| Leucine LEU | mg/100g | 60 |
| Lysine LYS | mg/100g | 62 |
| Phenylalanine PHE | mg/100g | 55 |
| Methionine MET | mg/100g | 27 |
| Threonine THR | mg/100g | 48 |
| Isoleucine ILE | mg/100g | 40 |
| Valine VAL | mg/100g | 49 |
| Arginine AGR | mg/100g | 84 |
| Serine SER | mg/100g | 47 |
| Glycine GLY | mg/100g | 52 |
| Alanine ALA | mg/100g | 51 |
| Cystine CYS | mg/100g | 17 |
| Tyrosine TYR | mg/100g | 41 |
| Histidine HIS | mg/100g | 14 |
| Proline PRO | mg/100g | 44 |
| Ammonia NH3 | mg/100g | 20 |
Tara vine light (seedling stage happiness yin) likes water, is fertilizer favorite.For suitable growth in good water-retaining property, gas permeability is strong, humus
In subacidity soil abundant.Under normal circumstances, fruit partially sees in tissue culturing seedling after being colonized 2 years, and field planting can be tied largely after 3 years
Fruit.Tara vine fruit between the axil that saves of the raw 4-12 in fruitful branch, every section fruit setting 1-3, mostly 3 fruits, fruit setting
Rate is up to 98% or more.It can be used as next year bearing basal shoot after retaining sufficient length pinching to the nutrition branch of strong prosperous growth.Current year is not
Extract the kryptoblast of fruitful branch out, next year remains to sprout fruitful branch flowering fruit out.Bearing basal shoot has stronger continuous bearing capacity.
The branch of excessive growth has indeterminate growth habit, in, fruit spur mostly stops growing in mid-June.The tara vine maturity period is early, is relatively early
Cooked food kind.Mu plants 67 plants, and per mu yield fresh fruit is up to 1500kg or more.
Tara vine tree body is resistant to -30.3 DEG C of low temperature, and winter resistance is strong, and it is cold-proof that winter does not have to undercarriage.But since level land plants
Training block and fall leaves without trees humus layer covering, it is identical as other fruit trees, trunk histocyte often because day and night temperature greatly and
It is larger away from cold wind in winter wind speed at the 1m of ground and destroyed, to cause tree body cold damage.Therefore, the overwintering needs pair of level land cultivation
Butt is dressed outside, cold-proof overwintering.Growing season likes warm and moist, the good weather conditions of illumination.Fear frost avoids non-irrigated fearness
Flood.Insect pest is mainly scale insect and fall webworms.Growing season pays attention to management of strengthening comprehension, and enhances tree body resistance, realizes efficiently raw
Produce target.
Embodiment 2
A kind of breeding method of tara vine, comprising the following steps:
S1 chooses the stem apex of tara vine as explant, and carry out disinfection to it inoculation；
S2 goes to after disinfection inoculation and carries out expanding numerous culture in subculture medium for 35 days；
S3, expanding after numerous culture 45 days will be in the tissue culture transplantation of seedlings to sand bed that taken root；
S4, transplanting is into nutritive cube after the tissue-cultured seedling in sand bed is grown 50 days；
S5, the outdoor cropping when the tissue-cultured seedling in nutritive cube grows to 40cm.
Trait expression is prominent after tara vine Gui Pu, and tree body branches and tendrils are sturdy, and tree vigo(u)r is smaller, can rational close planting to improve
Per mu yield.Winter resistance is strong, and winter occurs without any freeze injury.Based on middle long bearing basal shoot result, bud is easily formed, has continuous knot
Fruit ability, yielding ability are strong.Fruitful branch binds naturally, it is not necessary to pinching is carried out to fruitful branch, convenient for management.Fruit quality is excellent, fruit
Real surface is smooth, full of nutrition, and taste is strong, commodity function admirable.In fructescence evening, yield is high, suitable for making date-plum persimmon Mi
The deep processed products such as monkey peach ice wine.Tara vine meets breeding goal, tissue cultures is carried out to the fine individual plant, to obtain
The completely the same tissue culturing seedling with the kind inhereditary feature.
Tara vine fruit is subsphaeroidal, and emerald green, peel smooth is hairless, and skin depth 1.12cm, wax layer is obvious, fruit bottom
Color is yellow, no fruit dot.Stalk is hollow wide shallow, and stem has cyclic annular moth patch, the long 1.51-1.87cm of carpopodium.Calyx low-lying area harbors sub-wire capitate stigma,
Sepal falls off.Pulp light green.Core oblong, white indulge stem 1.95cm, transverse diameter 1.53cm.Each fruit seed average out to 168
Grain, seed brown, ellipse, mass of 1000 kernel 2.88g.Fresh fruit indulges diameter 2.81cm, transverse diameter 2.19cm, side diameter 1.92cm.Mean Fruit Weight
10.05g, maximum fruit weight 12.4g, nutrient constituents of fruit are as shown in table 2 below:
| Nutritional ingredient | Unit | Content |
| Vitamin C | mg/100g | 304 |
| Soluble solid | g/100g | 14.7 |
| Titratable acid | g/100g | 0.6 |
| Total reducing sugar | g/100g | 4.2 |
| Asparatate ASP | mg/100g | 228 |
| Glutamic acid GLU | mg/100g | 233 |
| Leucine LEU | mg/100g | 65 |
| Lysine LYS | mg/100g | 76 |
| Phenylalanine PHE | mg/100g | 55 |
| Methionine MET | mg/100g | 34 |
| Threonine THR | mg/100g | 59 |
| Isoleucine ILE | mg/100g | 56 |
| Valine VAL | mg/100g | 70 |
| Arginine AGR | mg/100g | 80 |
| Serine SER | mg/100g | 60 |
| Glycine GLY | mg/100g | 58 |
| Alanine ALA | mg/100g | 66 |
| Cystine CYS | mg/100g | 33 |
| Tyrosine TYR | mg/100g | 60 |
| Histidine HIS | mg/100g | 39 |
| Proline PRO | mg/100g | 83 |
| Ammonia NH3 | mg/100g | 43 |
Tara vine light (seedling stage happiness yin) likes water, is fertilizer favorite.For suitable growth in good water-retaining property, gas permeability is strong, humus
In subacidity soil abundant.Under normal circumstances, fruit partially sees in tissue culturing seedling after being colonized 2 years, and field planting can be tied largely after 3 years
Fruit.Tara vine fruit between the axil that saves of the raw 4-12 in fruitful branch, every section fruit setting 1-3, mostly 3 fruits, fruit setting
Rate is up to 98% or more.It can be used as next year bearing basal shoot after retaining sufficient length pinching to the nutrition branch of strong prosperous growth.Current year is not
Extract the kryptoblast of fruitful branch out, next year remains to sprout fruitful branch flowering fruit out.Bearing basal shoot has stronger continuous bearing capacity.
The branch of excessive growth has indeterminate growth habit, in, fruit spur mostly stops growing in mid-June.Tara vine fruitful branch binds naturally,
Pinching need not be carried out to fruitful branch, convenient for management.Its result quantities is big, evening in maturity period, is late variety.Mu plants 67 plants, and per mu yield is fresh
Fruit is up to 1500kg or more.
Tara vine tree body is resistant to -30.3 DEG C of low temperature, and winter resistance is strong, and it is cold-proof that winter does not have to undercarriage.But since level land plants
Training block and fall leaves without trees humus layer covering, it is identical as other fruit trees, trunk histocyte often because day and night temperature greatly and
It is larger away from cold wind in winter wind speed at the 1m of ground and destroyed, to cause tree body cold damage.Therefore, the overwintering needs pair of level land cultivation
Butt is dressed outside, cold-proof overwintering.Tara vine Growing season likes warm and moist, the good weather conditions of illumination.Fear
Frost avoids drought fearness flood.Insect pest is mainly scale insect and fall webworms.Growing season pays attention to management of strengthening comprehension, and enhances tree body resistance,
Realize efficient productive target.
Embodiment 3
A kind of breeding method of tara vine, comprising the following steps:
S1 chooses the stem apex of tara vine as explant, and carry out disinfection to it inoculation；
S2 goes to after disinfection inoculation and carries out expanding numerous culture in subculture medium for 30 days；
S3, expanding after numerous culture 40 days will be in the tissue culture transplantation of seedlings to sand bed that taken root；
S4, transplanting is into nutritive cube after the tissue-cultured seedling in sand bed is grown 45 days；
S5, the outdoor cropping when the tissue-cultured seedling in nutritive cube grows to 35cm.
Tara vine is staminiferous plant, and trait expression is prominent after Gui Pu, and tree body growing way is strong, and branches and tendrils are sturdy.Winter resistance is strong, the winter
Season occurs without any freeze injury.Bud is easily formed, flower amount is big, and the florescence is long.Tara vine meets breeding goal, to the fine individual plant
Tissue cultures are carried out, to obtain the tissue culturing seedling completely the same with the kind inhereditary feature.
Tara vine tree body is resistant to -30.3 DEG C of low temperature, and winter resistance is strong, and it is cold-proof that winter does not have to undercarriage.But since level land plants
Training block and fall leaves without trees humus layer covering, it is identical as other fruit trees, trunk histocyte often because day and night temperature greatly and
It is larger away from cold wind in winter wind speed at the 1m of ground and destroyed, to cause tree body cold damage.Therefore, the overwintering needs pair of level land cultivation
Butt is dressed outside, cold-proof overwintering.Growing season likes warm and moist, the good weather conditions of illumination.Fear frost avoids non-irrigated fearness
Flood.Insect pest is mainly scale insect and fall webworms.Growing season pays attention to management of strengthening comprehension, and enhances tree body resistance, realizes efficiently raw
Produce target.
The above are merely the preferred embodiment of the present invention, it is noted that for those skilled in the art, not
Under the premise of being detached from present inventive concept, several modifications and improvements can also be made, these also should be considered as protection model of the invention
It encloses, these all will not influence the effect and patent practicability that the present invention is implemented.
Claims (5)
1. a kind of breeding method of tara vine, which comprises the following steps:
S1 chooses the stem apex of tara vine as explant, and carry out disinfection to it inoculation；
S2 goes to after disinfection inoculation and carries out expanding numerous culture in subculture medium for 25-35 days；
S3, expanding after numerous culture 35-45 days will be in the tissue culture transplantation of seedlings to sand bed that taken root；
S4, transplanting is into nutritive cube after the tissue-cultured seedling in sand bed is grown 40-50 days；
S5, the outdoor cropping when the tissue-cultured seedling in nutritive cube grows to 30-40cm.
2. the breeding method of tara vine according to claim 1, which is characterized in that in step s 2, disinfection inoculation
It goes within 30 days afterwards and carries out expanding numerous culture in subculture medium.
3. the breeding method of tara vine according to claim 1 or 2, which is characterized in that in step s3, expand numerous training
It 40 days will be in the tissue culture transplantation of seedlings to sand bed that taken root after supporting.
4. the breeding method of tara vine according to claim 3, which is characterized in that in step s 4, in sand bed
Tissue-cultured seedling grow 45 days after transplanting into nutritive cube.
5. the breeding method of tara vine according to claim 4, which is characterized in that in step s 5, work as nutritive cube
In tissue-cultured seedling outdoor cropping when growing to 35cm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713410A (en) * | 2020-07-03 | 2020-09-29 | 四川农业大学 | Kiwi explant detoxification method |
| CN112997788A (en) * | 2021-02-04 | 2021-06-22 | 佳木斯大学 | Kiwi fruit facility cultivation method |
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| CN108077068A (en) * | 2016-11-21 | 2018-05-29 | 蒲江橙海阳光农业科技有限公司 | A kind of rapid propagation method of the sterile seedling of Kiwi berry |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112997788A (en) * | 2021-02-04 | 2021-06-22 | 佳木斯大学 | Kiwi fruit facility cultivation method |
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Application publication date: 20181221 |