The breeding method of Rosa roxburghii Tratt seedling
Technical field
The invention belongs to agricultural plantation technology fields, specifically disclose a kind of breeding method of Rosa roxburghii Tratt seedling.
Background technique
Rosa roxburghii Tratt also known as thatch pears, oblonga, alias send the spring to return, pierce sour pear, nine-headed bird, Wen Xianguo, are rosaceous plant reel silk from cocoons
The fruit of silk flower, is the nutrition treasure fruit of healthcare.Vitamin C rich in fruit, referred to as " king of Victoria C ".Rosa roxburghii Tratt
Medical value it is very high, flower, leaf fruit, seed can be used as medicine, and have stomach invigorating, help digestion, nourish, the effect of antidiarrheal.Especially Rosa roxburghii Tratt is rich in
Superoxide dismutase is internationally recognized with anti-ageing, protective effect on cancer risk active material, and Rosa roxburghii Tratt also has antiviral, anti-radiation
Effect, in terms of cardiovascular, digestive system and various tumor disease prevention and treatments, application is very extensive.Single Roxburgh Rose Fruit is sour, puckery, flat,
With enhancing body to the resistance of infection, cancer-resisting, treatment scurvy, lead-eliminating effect, anti-aging, treatment athlete's foot, control
It treats oral inflammation, promote human normal development and treatment yctalopia, invigorating the spleen is aid digestion solution pain, lower hyperlipidemia, hypertension, hyperglycemia and other effects.
Currently, Rosa roxburghii Tratt Guizhou Province carried out artificial growth last decade or so, because of the product development and marketing not
It is disconnected deeply, show that market development potential is huge, and development prospect is wide.The modes of reproduction of Rosa roxburghii Tratt includes sowing and cutting propagation two
Kind, in the reproductive process of seed sowing, seed will do it refrigerated storage after current year harvests, with sowing of waiting for the coming year.
Then need to carry out seed soaking process to promote advance germination of seeds, convenient for the breeding process in later period before sowing;And it soaks seed
In the process, people can not visually observe whether seed reaches requirement, and the seed soaking time such as seed is too long, and seed water suction occurs excessively,
Embryo is impaired;And the seed soaking time of seed is too short, neutron absorption water shortage, seed can not normally germinate.Therefore, correctly sentence
One of an important factor for whether disconnected seed-soaking reaches demand, is raising percentage of seedgermination.
Summary of the invention
The invention discloses a kind of breeding methods of Rosa roxburghii Tratt seedling, and its object is to improve seed when Rosa roxburghii Tratt seedling fostering
Germination percentage.
To solve the above problems, basic scheme of the invention are as follows:
The breeding method of Rosa roxburghii Tratt seedling, includes the following steps:
A. it chooses seeds: in Rosa roxburghii Tratt duration of flowering, choosing that flower is intact, growing way is vigorous, 4 years or more, the mature fruit tree without insect pest
As maternal plant;After having chosen maternal plant, it is protected, prevents the generation of insect pest, mechanical damage;It, will be small-sized after fruit tree fruiting
Fruit, disease fruit are wiped out in time;After fruit of Grossularia burejensis Berger is mature, the full picking fruit of golden yellow color, pulp is selected;Same day picking
Fruit, the same day carry out manually taking kind, it is ensured that seed is complete;
The seed of harvesting: being carried out drying processing 0.5d by b. seed treatment in the sun, then will dry the seed of moisture into
Row cryogenic freezing 4-5d;Seed storage after freezing is got up, while carrying out anti-mildew work;
C. it soaks seed: the seed freezed being taken out, is grouped, weighing label is carried out to each group of seed, by seed
It is placed in seed soaking case and carries out seed soaking;One group of seed is placed in a seed soaking device, seed soaking device is claimed
Amount carries out weight record;Seed soaking device is placed in seed-soaking liquid and is soaked seed;After seed soaking 12h, to the seed soaking device for removing moisture removal
It weighs, calculates the weight change of this batch of seed each group, judge whether each group reaches seed soaking demand in this batch of seed;Reach leaching
The seed of kind demand takes out from seed soaking device, and next group seed is placed into seed soaking device, is not up to demand with last consignment of
Seed is soaked seed again;
D. vernalization: being laid on plank using the mixture of fermentation organic fertilizer and plant ash and make seedbed, and seed soaking is completed
Seed afterwards is buried in seedbed, then carries out vernalization operation;35% seed can be sowed when showing money or valuables one carries unintentionally;
E. it sows: excavating gully in nursery, 30-35 centimetres of gully width, 0.5-1 centimetres of depth are uniform by seed
Be sprinkled upon in gully, then soil is backfilled, seed does not expose.
For compared with the existing technology:
1. taken in this programme measurement seed-soaking before with the weight change situation after seed soaking, to measure Rosa roxburghii Tratt seed
Water absorption can effectively meet the germination item of Rosa roxburghii Tratt seed when the water absorption of Rosa roxburghii Tratt seed reaches the 30-40% of own wt
Part can effectively improve the germination percentage of seed.
2. soaking seed simultaneously in this programme, measure, can in time take the seed for having completed seed soaking in batches
Out, it avoids seed from long soaking time occur, influences germination percentage;The seed for not completing seed soaking will not be influenced simultaneously.
Further, in seed treatment step, cryogenic freezing environment is 3-6 degrees Celsius of temperature, relative humidity 70-80%.
3-6 degrees Celsius of temperature, relative humidity 70-80% environment in seed carry out cryogenic freezing, can effectively make
Seed shifts to an earlier date hibernation, promotes seed germ differentiation.
Further, in step of soaking seed, seed-soaking liquid includes 35-42 degrees Celsius of warm water and carbendazim, and warm water is pressed with carbendazim
The ratio of volume ratio 1000:1 is stirred.
It is soaked seed using warm water to seed, awake kind is carried out to seed, promotes neutron absorption germination;Carbendazim is to kind simultaneously
Son is sterilized, and is avoided seed bacterial infection or microorganism during storage, is influenced the normal germination of seed.
Further, further include temperature adjustment step in vernalization step, seedbed is placed in 20-25 degrees Celsius, relative humidity 70-
In 80% environment.
Seed is in 20-25 degrees Celsius, in the environment of relative humidity 70-80%, meets the extraneous ring to germination
The demand in border avoids outside environmental elements from influencing the normal seedling of seed.
Further, sowing in step further includes fertilising step, and interval carries out single-dose application to nursery in two months;It applies every time
Fertilizer uses urea liquid, and the dosage of urea is 10kg-13kg/ mus.
It is applied fertilizer using urea to nursery, the growth of seedling can be effectively facilitated;The dosage of urea is limited simultaneously, is prevented
Only urea content is too low, can not provide enough nutrition to seedling;Urea content is excessively high, burn seedlings phenomenon occurs, causes seedling
It is dead.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of seed soaking case main view direction involved in the embodiment of the present invention;
Fig. 2 is the partial enlargement diagram in Fig. 1 at A.
Specific embodiment
It is further described below by specific embodiment:
Appended drawing reference in Figure of description includes: cabinet 1, hook 2, spring scale 3, cylinder 4, transverse slat 5, protruding block 6, the
The one seed soaking seed soaking of plate 7, second plate 8, elastic clip 9, water tank 10, cylinder 11, tracheae 12, through-hole 13, valve 14, air feed pump 15, branch
Bar 16, the first groove 71, the second groove 72.
Seed soaking case involved in embodiment is substantially as shown in Fig. 1: a kind of seed soaking case, the cabinet 1 including sidewall opening, case
The lower surface of 1 top plate of body is threaded with several hooks 2, is linked with spring scale 3 on hook 2.
The lower end of spring scale 3 is placed with hollow cylinder 4, and bracing wire is fixed on cylinder 4, and cylinder 4 hangs over bullet by bracing wire
On spring scale 3,4 top and bottom of cylinder are open, and tracheae 12 is slidably connected at 4 upper end port of cylinder, and tracheae 12 is bonded in top
On plate, valve 14 is installed on tracheae 12.
As shown in Figure 2: 4 bottom of cylinder is welded with transverse slat 5, and through-hole 13, the center line and cylinder of through-hole 13 are offered on transverse slat 5
The centerline collineation of body 4;The lower section of transverse slat 5 is welded with the protruding block 6 of face setting, is connected with seed soaking between two pieces of protruding blocks 6
Device.
Device of soaking seed includes the first seed soaking plate 7 and the second seed soaking plate 8, on the first seed soaking plate 7 integrally formed with several the
One groove 71, integrally formed with the second groove 72 on the second seed soaking plate 8, the first groove 71 and the setting of 72 face of the second groove, the
Permeable hole is provided on one groove 71 and the second groove 72.
Several struts 16 are welded on first seed soaking plate 7, the position that strut 16 is corresponded on the second seed soaking plate 8 is provided with circular hole,
The length dimension of strut 16 is less than Rosa roxburghii Tratt seed diameter size, and the length dimension of pillar is greater than the depth dimensions of circular hole;First leaching
It is provided with card slot on the left side wall of kind plate 7, is provided with identical card slot on the right side wall of the second seed soaking plate 8;First seed soaking plate 7 and second
Elastic clip 9 is installed on seed soaking plate 8.
The bottom bolts of cabinet 1 are connected with cylinder 11, and open-topped water tank 10, gas are welded on the piston rod of cylinder 11
The rodless cavity of cylinder 11 is connected to all tracheaes 12, and the bottom bolts of cabinet are connected with air feed pump 15.
Included the following steps: using seed soaking case
A. it chooses seeds: in Rosa roxburghii Tratt duration of flowering, choosing that flower is intact, growing way is vigorous, 4 years or more, the mature fruit tree without insect pest
As maternal plant;After having chosen maternal plant, it is protected, prevents the generation of insect pest, mechanical damage;It, will be small-sized after fruit tree fruiting
Fruit, disease fruit are wiped out in time;After fruit of Grossularia burejensis Berger is mature, the full picking fruit of golden yellow color, pulp is selected;Same day picking
Fruit, the same day carry out manually taking kind, it is ensured that seed is complete.
B. seed treatment: the seed of harvesting is carried out to dry processing 0.5d in the sun, then the seed for drying moisture is put
It sets in 4 degrees Celsius of temperature, 75% environment of relative humidity, carries out cryogenic freezing 4.5d;Seed storage after freezing is got up, together
When carry out anti-mildew work.
C. it soaks seed: the seed freezed being taken out, is grouped, weighing label is carried out to each group of seed, is recorded
The total weight G1 of every group of seed.
First seed soaking plate 7 is in the state that do not sling, and the first seed soaking plate 7 is laid flat, one group of seed is placed on first
It soaks seed on plate 7, is located at each seed in one the first groove 71, then the first leaching of circular hole alignment that second is soaked seed on plate 8
Strut 16 on kind plate 7, makes the second groove 72 and the first groove 71 face one by one;The second seed soaking plate 8 is firmly pushed down on again,
It covers the circular hole on the second seed soaking plate 8 on the strut 16 of the first seed soaking plate 7, closes the second seed soaking plate 8 lid in the first seed soaking plate 7
On.
The length dimension of pillar is greater than the depth dimensions of circular hole, therefore exists between the first seed soaking plate 7 and the second seed soaking plate 8
Gap, and the length dimension of pillar is less than Rosa roxburghii Tratt seed diameter size, therefore Rosa roxburghii Tratt seed is in the first groove 71 and the second groove
It, cannot be through being fallen away in gap in 72.
First seed soaking plate 7 and the second seed soaking plate 8 are clamped using elastic clip 9, form seed soaking device;It again will seed soaking
Device makes protruding block 6 block and in the first seed soaking plate 7 and the card slot of the second seed soaking, will soak seed across corresponding protruding block 6
Device is fixed, and spring scale 3 is observed, and records the sum of the weight of seed soaking device and seed G23;Every group of seed soaking device is fixed on pair
On the protruding block 6 answered, spring scale 3 is observed, the sum of every group of seed soaking device and weight of seed are recorded.
It is stirred with the ratio of 40 degrees Celsius of warm water and carbendazim 1000:1 by volume, seed-soaking liquid is made, will soak
Kind liquid is placed in water tank 10;Start air feed pump 15, air feed pump 15 to the rodless cavity delivery air of cylinder 11, cylinder 11 without bar
Intracavitary air pressure increases, and 11 piston plate of pressure push cylinder moves upwards, and 11 piston plate of cylinder drives piston plate to move upwards, cylinder 11
Piston rod push water tank 10 to move upwards, until seed-soaking liquid liquid level not have all seeds in water tank 10, closing air feed pump 15;Leaching
Kind liquid is contacted through permeable hole and gap with seed, carries out seed soaking to seed.
It soaks seed after 12h, opens the valve 14 on tracheae 12, the pressure inside 11 rodless cavity of cylinder is greater than the external world, leads to gas
Gas transtracheal 12 in 11 rodless cavity of cylinder flows out;The piston plate of cylinder 11 drives piston rod to move downward, the piston of cylinder 11
Bar drives water tank 10 to move downward, until water tank 10 moves to initial position, closes valve 14.
When cylinder 11 drives water tank 10 to move downward, the outflow of gas transtracheal 12 in 11 rodless cavity of cylinder generates air-flow;
Air-flow is blown into cylinder 4, and final air-flow is blown into the gap of seed soaking device via the through-hole 13 on transverse slat 5, by the first groove 71, the
The moisture that two grooves 72 and the surface of the seed have is blown away.
When air-flow blows seed soaking device, downward thrust is generated to seed soaking device, seed soaking device is made to overcome the bullet of spring scale
Power moves downward;Seed soaking device band motion plate 5 moves downward, and transverse slat 5 drives cylinder 4 to move downward, and cylinder 4 and tracheae 12 occur
Relative motion, 4 extension spring scale 3 of cylinder, deformation occurs for the spring for making in spring scale 3;When air-flow stops, the bullet of spring scale 3
Spring starts to reply deformation, and spring scale 3 pulls cylinder 4 to move upwards, and cylinder 4 is moved upwards with motion plate 5 and seed soaking device;Entirely
Process generates shake to seed soaking device, and the moisture accelerated on seed soaking device is fallen, and reduces influence of the residual moisture to measurement.
After the numerical value on spring scale 3 is no longer changed, seed soaking the sum of device and the weight of seed at this time is observed
G3.According to the metering method of (G3-G2)/G1, water absorption G4 is calculated.
When G4 is located at 30%-40%, this group of seed-soaking is completed, and is taken out this group of seed and is carried out vernalization, then by next group
Seed is put into the seed soaking device;When G4 is greater than 40%, this group of seed water absorption is excessive, takes out the group and discards, by next group
Seed is put into the seed soaking device;When G4 is less than 30%, this group of seed water absorption is insufficient, maintains original-pack constant.
After completing above-mentioned work, the seed-soaking liquid in water tank 10 is replaced;It is again started up cylinder 11, cylinder 11 pushes water
Case 10 moves upwards, until the liquid level of seed-soaking liquid did not had all seeds in water tank 10, closes cylinder 11, seed-soaking liquid carries out seed
Seed soaking.
D. vernalization: being laid on plank using the mixture of fermentation organic fertilizer and plant ash and make seedbed, and seed soaking is completed
Seed afterwards is buried in seedbed, and seedbed is placed in 22 degrees Celsius, in the environment of relative humidity 705%, then carries out vernalization
Operation;35% seed can be sowed when showing money or valuables one carries unintentionally;
E. it sows: excavating gully in nursery, seed is uniformly sprinkled upon by 32 centimetres of gully width, 0.6 centimetre of depth
In gully, then soil backfilled, seed does not expose;Interval carries out single-dose application to nursery in two months;Fertilising makes every time
With urea liquid, the dosage of urea is 11kg/ mus.
What has been described above is only an embodiment of the present invention, and common knowledge does not describe excessively herein in scheme.It should be pointed out that
For those skilled in the art, without departing from the structure of the invention, several modifications and improvements can also be made,
These also should be considered as protection scope of the present invention, these all will not influence the effect and patent practicability that the present invention is implemented.
The scope of protection required by this application should be based on the content of the claims, and the records such as specific embodiment in specification can
For explaining the content of claim.