CN109020688A - Anaerobism adds the method for aerobic fermentation production ecological organic fertilier - Google Patents

Anaerobism adds the method for aerobic fermentation production ecological organic fertilier Download PDF

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CN109020688A
CN109020688A CN201810945006.4A CN201810945006A CN109020688A CN 109020688 A CN109020688 A CN 109020688A CN 201810945006 A CN201810945006 A CN 201810945006A CN 109020688 A CN109020688 A CN 109020688A
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sugarcane
filter mud
culture
fertilizer
powder
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蒙建玲
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B13/00Fertilisers produced by pyrogenic processes from phosphatic materials
    • C05B13/02Fertilisers produced by pyrogenic processes from phosphatic materials from rock phosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Soil Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to organic fertilizers, more particularly to anaerobism plus the method for aerobic fermentation production ecological organic fertilier, using industrial filter mud of sugarcane as main material, using plant ash, cane molasses, calcium magnesium phosphate, rice bran, peat, potassium fulvate, bacteria agent and octagonal slag as auxiliary material;Composition with specific aerobic bacteria and anaerobic bacteria is biological modeling, overcome taking up a large area for normal fermentation bio-organic fertilizer, cost of investment is big, cost of labor is big, the advantages that environmental pollution is big, with saving of labor, environmental protection, fast and efficiently bio-organic fertilizer new technology, meet modern agricultural production needs.Gained bio-organic fertilizer is suitable for agricultural production plantation and is used as base fertilizer or fertilising, has improvement soil, and chesson promotes soil fertility, promotes the efficiency of crop quality.

Description

Anaerobism adds the method for aerobic fermentation production ecological organic fertilier
Technical field
The present invention relates to organic fertilizers, more particularly to anaerobism plus the method for aerobic fermentation production ecological organic fertilier.
Background technique
In the past 30 years, more and more agricultural experts and government department are all actively advocating reduction chemical fertilizer and pesticide in the world Amount of application, more organic fertilizers, develop bio-feritlizer.Early in 1972, International Agriculture tissue just set up " organic agriculture movement League of Nations ", to push the production and monitoring of pollution-free food in world wide.
China is a large agricultural country, and the chemical fertilizer in the world 30% has but been consumed in the arable land for possessing the world 10%.Big quantization On the one hand the use of fertile pesticide solves the count issue of grain, increases income;On the other hand it brings again even more serious Soil acidification, hardened, while the harmful substances such as chemicals, hormone, chlorine root, nitrite remain exceeded, soil micro-ecosystem Environment is destroyed, and arable land health is degenerated and safety is severely impacted, and largely threatens grain security and agricultural Sustainable development directly or indirectly endangers the living environment and health of the mankind.How to change the physical environment of soil, reduces The dosage of chemical fertilizer and pesticide restores the balance and health of soil ecosystem, is current China's ecological agriculture sustainable development Exhibition needs the critical issue solved.
Many research institutions in China are relatively broad to the research of aerobic fermentation strain and technique, the strain of industrialized production Also be produced as enterprise more and more and be converted into product, but its there are the drawbacks of gradually show with being becoming tight for environmental protection policy Come, more typical example is exactly the bioid processing of livestock and poultry cultivation waste and the reparation of soil degradation.
Traditional aerobic fermentation process needs to dry or dry, and in this process, a large amount of ammonia, nitrogen are thermally generated ammonia, ammonia Gas is evaporate into atmosphere, both causes secondary pollution to environment, has deviated from the objective of environmental protection, and reduces fertilizer efficiency.So aerobic The tupe of fermentation does not have sustainability, and has then effectively evaded above-mentioned drawback using anaerobic ferment process, Er Qieti The fertilizer efficiency of fertilizer after having risen conversion.So will be widelyd popularize in future, be popularized.
The most arable soil in China is all degenerated to some extent, just needs to repair.But peasant is year after year large dosage of It using bio-bacterial manure (some Vegetable areas, fruit tree area mu apply 1 ton or more), but does not solve effectively still, to find out its cause, these bacterium Fertilizer is all aerobic bacteria fermentation or spares product substantially.After soil ecology is unbalance, these aerobic bacterias enter the breeding energy after soil layer Power is greatly hindered, and due effect can not be played.So cause planting industry fertilizer it is more and more, yield, quality are more next Lower, soil-borne disease increasingly frequency is more and more obstinate.And the plot of anaerobic bacteria is used, because the unique production acid of anaerobic bacteria, production class are thin The characteristic of rhzomorph can be improved quickly, restore soil ecology, it is ensured that have the anti-environment of healthy root of the crop, both improve in this way Absorption of the roots of crops to nutrient, and enhance crop resistance, for realize ecologic planting, biological prevention and control laid one it is good Basis.
Summary of the invention
It is of the existing technology the purpose of the present invention is overcoming the problems, such as, a kind of anaerobism is provided, aerobic fermentation production ecology is added to have The method of machine fertilizer, for the purpose of solving industrial filter mud of sugarcane, with plant ash, cane molasses, calcium magnesium phosphate, rice bran, peat, yellow corruption Sour potassium, bacteria agent and octagonal slag are auxiliary material, and adjustment further realizes biodegradable processing under appropriate conditions, biology Anaerobism organic fertilizer is made base fertilizer suitable for agricultural production plantation or is used as top dressing, it, which has, improves soil, chesson, Soil fertility is promoted, the function of crop quality is promoted.
Technical solution of the present invention is as follows:
Ecological organic fertilier, the raw material including following parts by weight:
300~400 parts of filter mud of sugarcane,
80~120 parts of plant ash,
15~30 parts of cane molasses,
60~70 parts of calcium magnesium phosphate,
220~280 parts of rice bran,
180~220 parts of peat,
40~50 parts of potassium fulvate,
3~7 parts of bacteria agent,
0.1~0.5 part of octagonal slag,
The bacteria agent is aerobic bacteria and anaerobic bacteria 1:4 weight ratio composition;It is wherein aerobic fungi degradation starch gemma Bacillus;Anaerobic bacteria is lactobacillus acidophilus;
The plant ash is that stalk, wormwood, eucalyptus branch, camphor tree branch, pine tree branch, fernwort and/or firewood class burn the grass come Wood ash;
The octagonal slag is the residue that octagonal fruit or branches and leaves distill out drying after fennel oil;
Micro- fertile 0.01~0.03 part can be added when raw material mixes;Micro- fertilizer, be containing be dissolved in the manganese of water, iron, zinc, The compound of boron, copper and molybdenum element, element wt ratio are 5:2:1:0.5:0.1:0.01;
The bacterial screening of the P. amylolyticus:
(a), by filter mud of sugarcane stacking or it is put into the cement pit of opening, then uses water spray, makes its wet weight 50~60%, It banks up 20~30 days, stirs daily, it is made to come into full contact with air, rot to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(b), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200~500 mesh;
(c), nutrient agar is culture medium, and P. amylolyticus is first carried out test tube slant culture, is then cultivated again Typical P. amylolyticus, single colonie, the P. amylolyticus list that will be selected are screened after the scribing line culture of ware inclined-plane The bacterium colony nutrient agar that bran powder, octagonal ground-slag, sugarcane marrow powder is added, culture, successively temperature is 35 DEG C, 40 for culture DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, filter out 65 DEG C or more of P. amylolyticus;
(d), tame: in nutrient agar add filter mud of sugarcane shower water, then add various concentration calcium magnesium phosphate, Urea steps up the concentration screening domestication of calcium magnesium phosphate, urea, filters out the solution starch gemma for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
10~20g of peptone,
Beef extract 3~5g of powder,
10~15g of sodium chloride,
15~20g of agar,
Final pH 7.3 ± 0.2.
The bacterial screening of lactobacillus acidophilus:
(a), inflated with nitrogen before the medium sterilization, it is therefore an objective to remove the oxygen in culture medium, be mentioned for the growth of lactobacillus acidophilus For anaerobic environment.
Then plus water spray (b), filter mud of sugarcane is filled into crock or plastic tank, makes its wet weight 40~50%, sealing Keep its air tight, place 30 days or more, it rots to filter mud of sugarcane, extracts filter mud of sugarcane shower water;
(c), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200~500 mesh;
(d), nutrient agar is culture medium, and lactobacillus acidophilus is first carried out test tube slant culture, then carries out culture dish inclined-plane again Typical lactobacillus acidophilus is screened after scribing line culture, bran powder, eight is added in the lactobacillus acidophilus single colonie selected by single colonie The nutrient agar of angle ground-slag, sugarcane marrow powder, culture, successively temperature is 35 DEG C, 40 DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 for culture DEG C, filter out 65 DEG C or more of lactobacillus acidophilus.
(e), it tames: adding filter mud of sugarcane shower water in nutrient agar, then add the calcium and magnesium of various concentration Phosphate fertilizer, urea step up the concentration screening domestication of calcium magnesium phosphate, urea, filter out the acidophilus cream for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
10~15g of peptone,
Beef extract 3~5g of powder,
10~15g of sodium chloride,
10~15g of agar,
Final pH 7.3 ± 0.2.
To achieve the above object of the invention, method the following steps are included:
1, the pre-processing of filter mud of sugarcane: fresh cane filter mud isolated in sugar refinery 24 hours is removed into moisture, makes its water Divide and is no more than 40%;
2, peat is respectively crushed into powdery to 200~400 mesh standard sieves are crossed, then by filter mud of sugarcane, cane molasses, calcium and magnesium phosphorus Fertilizer, rice bran, peat powder and potassium fulvate ratio by weight mix;
3, it is 25%~40% that adjusting storeroom moisture, which is 25%~40%, C/N ratio,;Adjusting pH is 6~8;
4, biological bacteria composition is prepared:
Aerobic microbial inoculum is uniformly mixed with anaerobic bacteria by 1:4 weight ratio;
5, bio-organic fertilizer is prepared:
The polybag or anaerobic composting tower for charging material into sealing directly cure fermentation, and polybag or anaerobic composting tower are equipped with list To exhaust valve;Whole materials are made all to carry out anaerobic composting fermentation curing in the state of anaerobism, control temperature is lower than 60 DEG C, stacks Time be 25~30 days to obtain the final product;It is eventually adding plant ash and octagonal slag is uniformly mixed and obtains bio-organic fertilizer;
It is that the material that will be uniformly mixed is sent by conveyer belt to plastic knitting material to its week that anaerobic composting tower, which cures production process, While coping with its top when throwing full with substance in the conical tower slot sealed and being sealed with plastic knitting material.
Compared with prior art, present invention substantive distinguishing features outstanding and significant progress are:
1, filter mud of sugarcane
Guangxi mechanism candy output accounts for the last 2/3 of China's machine-processed sugar gross annual output amount, and sugaring industry is Guangxi economic rich and influential family out of question, It is environmental pollution rich and influential family.In today that environmental protection is increasingly reinforced, sugar refinery waste and pollutant are becoming restriction sugar The most important factor of industry development, if pollution can not be administered thoroughly, the waste pollutant in sugar refinery possibly even threatens sugar industry The existence of itself.
Cane sugar factory in addition to the major products such as white granulated sugar and brown granulated sugar, can also generate some waste materials in sugar production process, Such as bagasse, blackstrap, filter mud and heel, filter mud account for squeezing total amount 2%.Filter mud because water content height and being not easy ventilate etc. it is special Property, it is difficult to spontaneously dry, is very easy to by living contaminants, the filter mud that polluted enters in soil, more influence plant growth;Filter mud Arbitrarily stack, topple over and fill, not only land occupation resource, also pollution air, more polluted underground water source become filter mud The waste of " put no place to put, with cannot use ", high filter mud traffic expense and land seizure are paid in many sugar refinery thus every year again Take, filter mud becomes sugar refinery and environmental protection administration the difficult problem of environmental protection to be solved.Now with filter mud of sugarcane, crop can be become and absorbed The biological organic fertilizer utilized, turns waste into wealth.
The main component of filter mud is debris, phat fat, protein, minerals and a small amount of sugared part, and contains N P and K etc. A variety of nutritions that can be used as crop absorption, are activated it, organic substance therein are made further to recombinate humic Change, just become efficient, safety, good new bio-fertilizer, and meets organic, green agriculture production needs.
2, plant ash
Residue after plant (draft and xylophyta) burning, claims plant ash.Belong to insoluble material.Plant ash contains carbonic acid Potassium is capable of providing enough potash fertilizer to crop, and branch is more sturdy, and Plant ash fertilizer is the ash after burning plants because of plant ash Cinder nearly all contains in plant ash so being mineral element contained by all plants.Plant ash is light and in alkalinity, can be used to adjust The pH value of the organic fertilizer is saved, meanwhile, some plants also contain pest and evade agent, such as with wormwood, eucalyptus branch, camphor tree branch, pine The ashes of branch item firing can effectively make pest not damage to crops.
3, cane molasses
The cane molasses fermentation liquid obtained after cane molasses are fermented, except decomposed type organic matter, humic acid, life containing high concentration Change class fulvic acid, amino acid, outside high-absorbility NPK, it is right more containing the unknown growth factor [UGF] generated during the fermentation Plant can play the stimulation of the artificial synthetic hormones such as similar addition compound sodium nitrophenolate, methyl α-naphthyl acetate, organic fertilizer of the invention, sugarcane Biochemical class fulvic acid content is increased to 92% or so by molasses fermented liquid, can be effectively facilitated crop root prosperity, be enhanced the work of enzyme Property, increase the absorption to fertilizer;Unknown growth factor [UGF], it be by some natural hormones, natural Enzymes, small composite peptide and Functional protein composition can excite and promote the secretion of plant growth hormone, and play similar compound sodium nitrophenolate, methyl α-naphthyl acetate etc. to crop The stimulation of artificial synthesized hormone.It can also ferment in fertilizer and finally provide enough nutrition to anaerobic bacteria and aerobic bacteria.
4, octagonal slag
Octagonal slag is that illiciumverum distills out the later residue of anise-oil, and the illiciumverum is Magnoliacea plant, latin name (IlliciumverumHooK.f.) belong to aiphyllium, be the fragrant plant of China's specialty, be mainly distributed on Guangxi, Guangdong, expensive The provinces and regions such as state, Yunnan.Guangxi is the major production areas of China's illiciumverum, and yield accounts for the 85% of the whole nation, accounts for the 70% of the world, Chinese anise at For important one of the economic tree in local mountain area.Octagonal and star anise oil is the excellent food seasoning of people's happiness, star anise oil tool There are the pungent fragrant fragrance of anise, also known as Chinese anise, it is other than being used as seasoning spice, in food industry, brewery industry, beverage Have been widely used in industry, daily cosmetics and pharmaceutical industry.Chinese anise is from octagonal branches and leaves or fruit through water vapour It distills and obtains, be both needed to prune to Chinese anise every year, repair to keep Chinese anise fruit bearing rate high according to Chinese anise cultivation technique The branches and leaves to get off can be used to extract star anise oil.Since the place of production has inconvenient traffic, based on peasant is mostly distilled with local method, this method by , production simple in equipment is easy, is at low cost, thus is used widely.But local method distillation efficiency is not high, and branches and leaves oil yield is only It is 0.8% or so;It distills later residue all to abandon substantially, even if current fragrance processing factory, although oil yield is more than 2- 3% or more, but rectifying cauldron bottom residue is not also recycled, the filtered raffinate in bottom after illiciumverum branches and leaves per ton or fruit rectifying There are also 5 kilograms or more.
In fact, Chinese anise rectifying cauldron bottom residue is a kind of resource being of great value, wherein more important is α, β-insatiable hunger And ketone, α, alpha, beta-unsaturated ketone are widely used, and since its carbon-carbon double bond and carbonyl are conjugated, therefore have alkene, ketone and conjugated diene concurrently Property, have good reactivity worth, such as carry out Michael addition reaction, Diels-Alder react.Furthermore it is still A kind of important organic synthesis intermediate, is widely used in the fields such as medicine, pesticide, fragrance.It is synthesis flavone compound Important intermediate, the present inventor have found that it can evade the pests such as aphid, red spider, cutworm through overtesting.
5, calcium magnesium phosphate
Calcium magnesium phosphate also known as melts the calcium-magnesium of fused containing magnesium-phosphorus fertilizer phosphate (fertilizer), is One kind containing the sillico aluminate glass body of phosphate radical (PO43-).Main component includes Ca3(PO4) 2, CaSiO3, MgSiO3.It is A kind of multi-element fertilizer, aqueous solution can improve acid soil in alkalinity, plant energy fine as base fertilizer effect when cultivating large seedling Nutrient needed for enough slow-absorbings.The inorganic fertilizer for belonging to ecology, will not destroy soil texture.
Calcium magnesium phosphate is celadon or taupe brown powder, and phosphorus content is 12%~18%, and main component is to be dissolved in citric acid α-Ca3 (PO4) 2, also contain the elements such as magnesium and a small amount of silicon.Magnesium is to the advantageous (important component of chlorophyll molecule of formation chlorophyll It is C55H72O5N4Mg and C55H70O6N4Mg), silicon can promote the growth of plant fiber tissue, and having plant preferably prevents down The ability of volt and pest and disease damage.Fine as base fertilizer effect when cultivating large seedling, plant being capable of nutrient needed for slow-absorbing.
6, rice bran
Various nutrients and physiological activator are rich in rice bran, rice bran can ferment in the soil, there is fertilizer increase largely Machine matter, chesson reduce soil hardening and may prevent the generation of pest and disease damage.
7, peat
Peat is a kind of by with being formed by atmospheric swamp product (also known as turf or mud coal) of thousands of years, is degree of coalification Minimum coal, while being also the state of coal most original, sterile, nontoxic, pollution-free, venting capability is good.
Light, water holding, fertilizer conservation are conducive to microbial activities, enhance biological property, full of nutrition, be both cultivation matrix and It is good soil mediator agent, and contains very high organic matter, humic acid and nutrition.
8, potassium fulvate
Fulvic acid is a kind of short carbon chain molecular structure substance extracted from natural humic acid.It has high load amount and physiology Activity.Applied to agricultural and Horticultural industry, have the advantage that chelating constant and trace nutrient make it preferably be plant Object utilizes;Controlling plant diseases enhance Flood resistance;Excite plant microbiological activity;Slow release fertilizer improves chemical fertilizer and pesticide benefit With;Nutrient absorption is improved, plant germination and growth is promoted;Accelerate precipitating to decompose, improves soil texture.
Humic acid can promote the activity of edaphon, increase the quantity of edaphon, enhance the activity etc. of soil enzyme The fact confirms by domestic and international numerous studies data, and unanimously thinks, application humic acid make aerophile bacterium, actinomyces, The quantity of cellulose-decomposing bacteria increases more.To the mineralising for accelerating organic matter, promote the release of nutrient advantageous.Therefore, it applies Humic acid can prevent and treat root rot, yellow leaf, the leaflet, wilt disease of crop.
Potassium fulvate is a kind of pure natural minerals activity potassium element fertilizer, potassium fulvate include microelement, rare earth element, The multiple nutritional components such as plant growth regulator, viral inhibitors, make nutrient it is more sufficient, supply it is more reasonable, so as to avoid work Object due to lacking element caused by various physiological disturbances generation, keep the more vigorous leaf color of crop plant type more dark green, energy resistant to lodging Power is stronger.Potassium fulvate can timely supplement the nutrient being lost in soil, make soil activating, have vitality, reduce soil Nutrient continuous cropping disease caused by taken in excess in earth, product can replace the identical potassium sulfate of content or potassium chloride and sulphur completely Sour potassium magnesium, and natural, environmental protection.
8, bacteria agent
Bacteria agent, can be changed that saliferous in soil is excessively high, too strong, grogs high degree of dispersion, soil texture difference the physics and chemistry of alkalinity Shape promotes the formation of soil agreegate, makes it at kilter, to create good condition for plant root growth development. Can loose topsoil soils, destroy the accumulation of salt, reduce surface soil salt content, play every salt action, improve emergence rate, reduce weak Seedling, dead seedling, make plant health grow up.The secretion of bacteria agent cationic converted quantity with higher is higher than general soil by 10 Times or more.It after being manured into soil, significantly improves soil to Anion-adsorption ability, reduces surface layer salt content.
Saline and alkaline soil especially alkaline earth pH value is excessively high (9.0 or more), direct damage to crops growth, or even causes death, separately The validity of the nutrients such as the excessively high phosphorus also affected in soil of outer pH value, iron, manganese, boron, zinc.And bacteria agent can neutralize alkali Property, mitigate the harm for eliminating alkalinity to soil and plant, improve low yield salt-soda soil, is created for crop seedling growth good Edaphic condition.
The secretion of bacteria agent efficient functional group containing there are many, the soil of the flora containing beneficial organism after being activated is to work Object growth and development and body physiological are metabolized irritating effect, and this characteristic is not available for general fertilizers, efficient after activation The physiological activity of seed, crop can be improved in bioactive substance, by a certain concentration using seed soaking, leaching root, root dipping, spray wine, pouring, The modes such as base fertilizer are done, have apparent effect of stimulation to various crops.General performance is in the promotion to root system development, to making produce The good influence of amount, qualitative factor.
Bacteria agent has special facilitation to the root system development of crop, and many agriculture section personnel's bacteria agents are referred to as " root It is fertilizer ", the influence to root system is mainly manifested in, and the division and growth of root apical meristem cell are stimulated, keeps seedling root of hair fast, Secondary root increases, and root amount increases, root elongation, and the ability for eventually leading to crop absorption moisture and nutrient greatly enhances.
On the basis of nutrient is in liberal supply, bacteria agent secretion polypeptide, enzyme can stimulation with can making plant Upper part Vegetative growth is vigorous, shows plant height, stem thickness, the number of blade, dry-matter accumulation etc..
After the special secretion of bacteria agent enters in plant, stimulation is played to plant, is mainly manifested in, breathed The activity enhancing of the increase of intensity, photosynthetic increase, various enzymes obtains high yield, height so that fruit be made to colour maturation in advance Value (the being put on sale property of industrial crops).
The application of bacteria agent can be such that plant leaf blade stomatal opening degree reduces, and leaf transpiration amount reduces, and water consumption is reduced, The intracorporal water regime of plant is improved, and leaf water content is improved, thus to divide fringe and laying a good foundation in seed, biological bacteria The application of agent can also improve chlorophyll content and it is photosynthetic be normally carried out, the accumulation of substance and mass of 1000 kernel are mentioned Height is very important.
Bacteria agent can effectively prevent underground pest and disease damage and plant disease and germ.It is a large amount of according to Shandong, Hebei, Shanxi Development test proves that bacteria agent is to the rotten of crop, leaflet, yellowtop, the downy mildew of cucumber, the anthracnose of capsicum, virus Disease etc. has the effect of obvious prevention and treatment and restraining and sterilizing bacteria.
With the raising of human living standard, not requiring nothing more than crops has high yield, and requires good product Matter characteristic.The quality of agricultural product is influenced by many factors, and most important factor is exactly fertilizer, scientific research personnel and vast agriculture The people sum up an experience in long-term practice, apply bacteria agent, not only there is apparent effect of increasing production and economic benefit, but also Bacteria agent green, environmental protection, substantially improve the quality for improving agricultural product, are the staple products of green basic industry.
9, micro- fertilizer is by a large amount of scientific experimentation and research, it has already been proven that with certain biological significance, plant is just It is frequently grown those of indispensable micronutrient element of development, is agriculturally being used as the chemical products of fertilizer application, in soil times A kind of what quick-acting state microelement is insufficient, and crop just will appear special symptom, and yield is reduced, quality decline, or even is received At hopeless.
10, the organic waste that the present invention is generated with domestic waste, agricultural crop straw, agricultural and sideline product and food processing For raw material, it is equipped with Multifunctional fermentation inoculum agent, the organic fertilizer containing a certain amount of functional microbial being process.
11, it is a kind of biological soil conditioner, soil fertility can be improved, make the normal ecological balance of soil restoration, increases Soil organic matter content forms crumb structure, improves Soil ventilation, water conservation, fertilizer conservation performance, promotes the growth of crop root, mention High and improvement plant quality and yield.
12, the present invention it is nontoxic, harmless, using safe, effectively improve chemical fertilizer utilization ratio, decomposing pesticide residual improves crop Matter of heap of stone, negatively produces pollution-free food.Reduce and causes agricultural product to caused by human body and birds and beasts because chemical fertilizer application is excessive Harm.
13, inorganic, the organic nutrient contained in itself can directly provide corps nutrient, be conducive to the absorption and quality of crop Raising, enhance the activity of soil enzyme.The ability of plant disease-resistant insect pest can be enhanced, crop rhizosphere beneficial microorganism is promoted to increase It grows, improves crop rhizosphere ecological environment.The increase of beneficial microorganism mass propagation and disease-resistant factor, in plant rhizosphere region shape At dominant population, it is suppressed that the growth and breeding of pathogenic microorganism reduces the generation of the lame insect pest of plant, and beneficial microbe can induce Plant generates Antibiotics substance and catalase, the polyphenol oxidase of plant etc., participates in the defense reaction of plant, mentions high fringe The ability of the disease and insect resistance of object, beneficial microbe secrete a variety of metabolites, the harmful micro- life of antagonism to soil in reproductive process Object can promote the conversion of nutrient in soil, improve the validity of soil nutrient, improve the nutritional condition of crop, increase soil fertilizer Power.
14, overcome taking up a large area for normal fermentation bio-organic fertilizer, cost of investment is big, and cost of labor is big, and environment is dirty The advantages that big is contaminated, there is saving of labor, environmental protection, fast and efficiently bio-organic fertilizer new technology, meet modern agricultural production needs. Gained bio-organic fertilizer is suitable for agricultural production plantation and is used as base fertilizer, has improvement soil, and chesson promotes soil fertilizer Power promotes the efficiency of crop quality.
Specific embodiment
Embodiment 1:
Ecological organic fertilier, the raw material including following parts by weight: 300 parts of filter mud of sugarcane, 80 parts of plant ash, 15 parts of cane molasses, 60 parts of calcium magnesium phosphate, 220 parts of rice bran, 180 parts of peat, 40 parts of potassium fulvate, 3 parts of bacteria agent, 0.1 part of octagonal slag;Plant ash The plant ash come is burnt for wormwood;Micro- fertilizer be containing the compound for being dissolved in the manganese of water, iron, zinc, boron, copper and molybdenum element, manganese sulfate, Ferrous sulfate, zinc sulfate, sodium tetraborate, copper sulphate, ammonium molybdate, element wt ratio are 5:2:1:0.5:0.1:0.01;
The bacterial screening of the P. amylolyticus:
(a), by filter mud of sugarcane stacking or it is put into the cement pit of opening, then uses water spray, makes its wet weight 50%, bank up It 20 days, stirs daily, it is made to come into full contact with air, rot to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(b), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200 mesh;
(c), nutrient agar is culture medium, and P. amylolyticus is first carried out test tube slant culture, is then cultivated again Typical P. amylolyticus, single colonie, the P. amylolyticus list that will be selected are screened after the scribing line culture of ware inclined-plane The bacterium colony nutrient agar that bran powder, octagonal ground-slag, sugarcane marrow powder is added, culture, successively temperature is 35 DEG C, 40 for culture DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, filter out 65 DEG C or more of P. amylolyticus;
(d), tame: in nutrient agar add filter mud of sugarcane shower water, then add various concentration calcium magnesium phosphate, Urea steps up the concentration screening domestication of calcium magnesium phosphate, urea, filters out the solution starch gemma for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
The bacterial screening of lactobacillus acidophilus:
(a), inflated with nitrogen before the medium sterilization, it is therefore an objective to remove the oxygen in culture medium, be mentioned for the growth of lactobacillus acidophilus For anaerobic environment.
Then plus water spray (b), filter mud of sugarcane is filled into crock or plastic tank, makes its wet weight 40%, sealing makes it It is air tight, it places 30 days, rots to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(c), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200 mesh;
(d), nutrient agar is culture medium, and lactobacillus acidophilus is first carried out test tube slant culture, then carries out culture dish inclined-plane again Typical lactobacillus acidophilus is screened after scribing line culture, bran powder, eight is added in the lactobacillus acidophilus single colonie selected by single colonie The nutrient agar of angle ground-slag, sugarcane marrow powder, culture, successively temperature is 35 DEG C, 40 DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 for culture DEG C, filter out 65 DEG C or more of lactobacillus acidophilus.
(e), it tames: adding filter mud of sugarcane shower water in nutrient agar, then add the calcium and magnesium of various concentration Phosphate fertilizer, urea step up the concentration screening domestication of calcium magnesium phosphate, urea, filter out the acidophilus cream for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
Its method the following steps are included:
1, the pre-processing of filter mud of sugarcane
Fresh cane filter mud isolated in sugar refinery 24 hours is removed into moisture, its moisture is made to be no more than 40%;
2, peat is respectively crushed into powdery to 200 mesh standard sieves are crossed, then by filter mud of sugarcane, cane molasses, calcium magnesium phosphate, rice Chaff, peat powder and potassium fulvate mix in proportion;
3, it is 30% that adjusting storeroom moisture, which is 25%, C/N ratio,;Adjusting pH is 8;
4, biological bacteria composition is prepared
Aerobic microbial inoculum is uniformly mixed with anaerobic bacteria by 1:4 weight ratio;
5, bio-organic fertilizer is prepared
The polybag or anaerobic composting tower for charging material into sealing directly cure fermentation, and polybag or anaerobic composting tower are equipped with list To exhaust valve;Whole materials are made all to carry out anaerobic composting fermentation curing in the state of anaerobism, control temperature is lower than 60 DEG C, stacks Time to obtain the final product, was eventually adding plant ash and octagonal slag is uniformly mixed and obtains bio-organic fertilizer for 25 days;
It is that the material that will be uniformly mixed is sent by conveyer belt to plastic knitting material to its week that anaerobic composting tower, which cures production process, While coping with its top when throwing full with substance in the conical tower slot sealed and being sealed with plastic knitting material.
Embodiment 2:
Bio anaerobic organic fertilizer is made of the raw material of following parts by weight: 350 parts of filter mud of sugarcane, 100 parts of plant ash, and cane suger 20 parts of honey, 65 parts of calcium magnesium phosphate, 240 parts of rice bran, 200 parts of peat, 45 parts of potassium fulvate, 5 parts of bacteria agent, octagonal slag 0.3 Part;Plant ash is that eucalyptus branch burns the plant ash come;Micro- fertilizer is the change containing the manganese, iron, zinc, boron, copper and molybdenum element for being dissolved in water Object, manganese sulfate, ferrous sulfate, zinc sulfate, sodium tetraborate, copper sulphate, ammonium molybdate are closed, element wt ratio is 5:2:1:0.5: 0.1:0.01;
The bacterial screening of the P. amylolyticus:
(a), by filter mud of sugarcane stacking or it is put into the cement pit of opening, then uses water spray, makes its wet weight 60%, bank up It 25 days, stirs daily, it is made to come into full contact with air, rot to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(b), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 500 mesh;
(c), nutrient agar is culture medium, and P. amylolyticus is first carried out test tube slant culture, is then cultivated again Typical P. amylolyticus, single colonie, the P. amylolyticus list that will be selected are screened after the scribing line culture of ware inclined-plane The bacterium colony nutrient agar that bran powder, octagonal ground-slag, sugarcane marrow powder is added, culture, successively temperature is 35 DEG C, 40 for culture DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, filter out 65 DEG C or more of P. amylolyticus;
(d), tame: in nutrient agar add filter mud of sugarcane shower water, then add various concentration calcium magnesium phosphate, Urea steps up the concentration screening domestication of calcium magnesium phosphate, urea, filters out the solution starch gemma for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
The bacterial screening of lactobacillus acidophilus:
(a), inflated with nitrogen before the medium sterilization, it is therefore an objective to remove the oxygen in culture medium, be mentioned for the growth of lactobacillus acidophilus For anaerobic environment.
Then plus water spray (b), filter mud of sugarcane is filled into crock or plastic tank, makes its wet weight 45%, sealing makes it It is air tight, it places 30 days, rots to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(c), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 500 mesh;
(d), nutrient agar is culture medium, and lactobacillus acidophilus is first carried out test tube slant culture, then carries out culture dish inclined-plane again Typical lactobacillus acidophilus is screened after scribing line culture, bran powder, eight is added in the lactobacillus acidophilus single colonie selected by single colonie The nutrient agar of angle ground-slag, sugarcane marrow powder, culture, successively temperature is 35 DEG C, 40 DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 for culture DEG C, filter out 65 DEG C or more of lactobacillus acidophilus.
(e), it tames: adding filter mud of sugarcane shower water in nutrient agar, then add the calcium and magnesium of various concentration Phosphate fertilizer, urea step up the concentration screening domestication of calcium magnesium phosphate, urea, filter out the acidophilus cream for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
Its method the following steps are included:
1, the pre-processing of filter mud of sugarcane
Fresh cane filter mud isolated in sugar refinery 24 hours is removed into moisture, its moisture is made to be no more than 40%;
2, peat is respectively crushed into powdery to 300 mesh standard sieves are crossed, then by filter mud of sugarcane, cane molasses, calcium magnesium phosphate, rice Chaff, peat powder and potassium fulvate mix in proportion;
3, it is 30% that adjusting storeroom moisture, which is 30%, C/N ratio,;Adjusting pH is 7;
4, biological bacteria composition is prepared
Aerobic microbial inoculum is uniformly mixed with anaerobic bacteria by 1:4 weight ratio;
5, bio-organic fertilizer is prepared
The polybag or anaerobic composting tower for charging material into sealing directly cure fermentation, and polybag or anaerobic composting tower are equipped with list To exhaust valve;Whole materials are made all to carry out anaerobic composting fermentation curing in the state of anaerobism, control temperature is lower than 60 DEG C, stacks Time to obtain the final product, was eventually adding plant ash and octagonal slag is uniformly mixed and obtains bio-organic fertilizer for 30 days;
It is that the material that will be uniformly mixed is sent by conveyer belt to plastic knitting material to its week that anaerobic composting tower, which cures production process, While coping with its top when throwing full with substance in the conical tower slot sealed and being sealed with plastic knitting material.
Embodiment 3:
Bio anaerobic organic fertilizer is made of the raw material of following parts by weight: 370 parts of filter mud of sugarcane, 110 parts of plant ash, and cane suger 25 parts of honey, 67 parts of calcium magnesium phosphate, 260 parts of rice bran, 210 parts of peat, 47 parts of potassium fulvate, 7 parts of bacteria agent, octagonal slag 0.4 part;Plant ash is that camphor tree branch burns the plant ash come;Micro- fertilizer is containing manganese, iron, zinc, boron, copper and the molybdenum element for being dissolved in water Compound, manganese sulfate, ferrous sulfate, zinc sulfate, sodium tetraborate, copper sulphate, ammonium molybdate, element wt ratio be 5:2:1: 0.5:0.1:0.01;
The bacterial screening of the P. amylolyticus:
(a), by filter mud of sugarcane stacking or it is put into the cement pit of opening, then uses water spray, makes its wet weight 55%, bank up It 30 days, stirs daily, it is made to come into full contact with air, rot to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(b), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200 mesh;
(c), nutrient agar is culture medium, and P. amylolyticus is first carried out test tube slant culture, is then cultivated again Typical P. amylolyticus, single colonie, the P. amylolyticus list that will be selected are screened after the scribing line culture of ware inclined-plane The bacterium colony nutrient agar that bran powder, octagonal ground-slag, sugarcane marrow powder is added, culture, successively temperature is 35 DEG C, 40 for culture DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, filter out 65 DEG C or more of P. amylolyticus;
(d), tame: in nutrient agar add filter mud of sugarcane shower water, then add various concentration calcium magnesium phosphate, Urea steps up the concentration screening domestication of calcium magnesium phosphate, urea, filters out the solution starch gemma for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
The bacterial screening of lactobacillus acidophilus:
(a), inflated with nitrogen before the medium sterilization, it is therefore an objective to remove the oxygen in culture medium, be mentioned for the growth of lactobacillus acidophilus For anaerobic environment.
Then plus water spray (b), filter mud of sugarcane is filled into crock or plastic tank, makes its wet weight 45%, sealing makes it It is air tight, it places 30 days, rots to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(c), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200 mesh;
(d), nutrient agar is culture medium, and lactobacillus acidophilus is first carried out test tube slant culture, then carries out culture dish inclined-plane again Typical lactobacillus acidophilus is screened after scribing line culture, bran powder, eight is added in the lactobacillus acidophilus single colonie selected by single colonie The nutrient agar of angle ground-slag, sugarcane marrow powder, culture, successively temperature is 35 DEG C, 40 DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 for culture DEG C, filter out 65 DEG C or more of lactobacillus acidophilus.
(e), it tames: adding filter mud of sugarcane shower water in nutrient agar, then add the calcium and magnesium of various concentration Phosphate fertilizer, urea step up the concentration screening domestication of calcium magnesium phosphate, urea, filter out the acidophilus cream for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
Its method the following steps are included:
1, the pre-processing of filter mud of sugarcane
Fresh cane filter mud isolated in sugar refinery 24 hours is removed into moisture, its moisture is made to be no more than 40%;
2, peat is respectively crushed into powdery to 400 mesh standard sieves are crossed, then by filter mud of sugarcane, cane molasses, calcium magnesium phosphate, rice Chaff, peat powder and potassium fulvate mix in proportion;
3, it is 40% that adjusting storeroom moisture, which is 25%, C/N ratio,;Adjusting pH is 8;
4, biological bacteria composition is prepared
Aerobic microbial inoculum is uniformly mixed with anaerobic bacteria by 1:4 weight ratio;
5, bio-organic fertilizer is prepared
The polybag or anaerobic composting tower for charging material into sealing directly cure fermentation, and polybag or anaerobic composting tower are equipped with list To exhaust valve;Whole materials are made all to carry out anaerobic composting fermentation curing in the state of anaerobism, control temperature is lower than 60 DEG C, stacks Time to obtain the final product, was eventually adding plant ash and octagonal slag is uniformly mixed and obtains bio-organic fertilizer for 25 days;
It is that the material that will be uniformly mixed is sent by conveyer belt to plastic knitting material to its week that anaerobic composting tower, which cures production process, While coping with its top when throwing full with substance in the conical tower slot sealed and being sealed with plastic knitting material.
Embodiment 4:
Bio anaerobic organic fertilizer is made of the raw material of following parts by weight: 400 parts of filter mud of sugarcane, 120 parts of plant ash, and cane suger 30 parts of honey, 70 parts of calcium magnesium phosphate, 280 parts of rice bran, 220 parts of peat, 50 parts of potassium fulvate, 7 parts of bacteria agent, octagonal slag 0.5 Part;Plant ash is that camphor tree branch burns the plant ash come;Micro- fertilizer is containing manganese, iron, zinc, boron, copper and the molybdenum element for being dissolved in water Compound, manganese sulfate, ferrous sulfate, zinc sulfate, sodium tetraborate, copper sulphate, ammonium molybdate, element wt ratio are 5:2:1:0.5: 0.1:0.01;
The bacterial screening of the P. amylolyticus:
(a), by filter mud of sugarcane stacking or it is put into the cement pit of opening, then uses water spray, makes its wet weight 60%, bank up It 30 days, stirs daily, it is made to come into full contact with air, rot to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(b), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 500 mesh;
(c), nutrient agar is culture medium, and P. amylolyticus is first carried out test tube slant culture, is then cultivated again Typical P. amylolyticus, single colonie, the P. amylolyticus list that will be selected are screened after the scribing line culture of ware inclined-plane The bacterium colony nutrient agar that bran powder, octagonal ground-slag, sugarcane marrow powder is added, culture, successively temperature is 35 DEG C, 40 for culture DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, filter out 65 DEG C or more of P. amylolyticus;
(d), tame: in nutrient agar add filter mud of sugarcane shower water, then add various concentration calcium magnesium phosphate, Urea steps up the concentration screening domestication of calcium magnesium phosphate, urea, filters out the solution starch gemma for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
The bacterial screening of lactobacillus acidophilus:
(a), inflated with nitrogen before the medium sterilization, it is therefore an objective to remove the oxygen in culture medium, be mentioned for the growth of lactobacillus acidophilus For anaerobic environment.
Then plus water spray (b), filter mud of sugarcane is filled into crock or plastic tank, makes its wet weight 50%, sealing makes it It is air tight, it places 30 days, rots to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(c), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 500 mesh;
(d), nutrient agar is culture medium, and lactobacillus acidophilus is first carried out test tube slant culture, then carries out culture dish inclined-plane again Typical lactobacillus acidophilus is screened after scribing line culture, bran powder, eight is added in the lactobacillus acidophilus single colonie selected by single colonie The nutrient agar of angle ground-slag, sugarcane marrow powder, culture, successively temperature is 35 DEG C, 40 DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 for culture DEG C, filter out 65 DEG C or more of lactobacillus acidophilus.
(e), it tames: adding filter mud of sugarcane shower water in nutrient agar, then add the calcium and magnesium of various concentration Phosphate fertilizer, urea step up the concentration screening domestication of calcium magnesium phosphate, urea, filter out the acidophilus cream for being resistant to higher concentration Bacillus;
The composition of the nutrient agar:
Peptone 10g,
Beef extract powder 3g,
Sodium chloride 15g,
Agar 15g,
Final pH 7.3.
Its method the following steps are included:
1, the pre-processing of filter mud of sugarcane
Fresh cane filter mud isolated in sugar refinery 24 hours is removed into moisture, its moisture is made to be no more than 40%;
2, peat is respectively crushed into powdery to 200~400 mesh standard sieves are crossed, then by filter mud of sugarcane, cane molasses, calcium and magnesium phosphorus Fertilizer, rice bran, peat powder and potassium fulvate mix in proportion;
3, it is 40% that adjusting storeroom moisture, which is 40%, C/N ratio,;Adjusting pH is 8;
4, biological bacteria composition is prepared
Aerobic microbial inoculum is uniformly mixed with anaerobic bacteria by 1:4 weight ratio;
5, bio-organic fertilizer is prepared
The polybag or anaerobic composting tower for charging material into sealing directly cure fermentation, and polybag or anaerobic composting tower are equipped with list To exhaust valve;Whole materials are made all to carry out anaerobic composting fermentation curing in the state of anaerobism, control temperature is lower than 60 DEG C, stacks Time to obtain the final product, was eventually adding plant ash and octagonal slag is uniformly mixed and obtains bio-organic fertilizer for 25 days;
It is that the material that will be uniformly mixed is sent by conveyer belt to plastic knitting material to its week that anaerobic composting tower, which cures production process, While coping with its top when throwing full with substance in the conical tower slot sealed and being sealed with plastic knitting material.
Rice Cropping of the invention tests (spreading fertilizer over the fields before flowering, 100 kilograms per acre)
Above table is the fraction of embodiment, compares according to the organic fertilizer bought in the market, applies fertilizer of the present invention Crop, yield greatly improve, and pest control effect is obvious, crop non-lodging.Other embodiments are also to be imitated by comparison to crop Fruit is significant.

Claims (9)

1. anaerobism adds the method for aerobic fermentation production ecological organic fertilier, which is characterized in that the raw material including following parts by weight:
300~400 parts of filter mud of sugarcane;
80~120 parts of plant ash;
15~30 parts of cane molasses;
60~70 parts of calcium magnesium phosphate;
220~280 parts of rice bran;
180~220 parts of peat;
40~50 parts of potassium fulvate;
3~7 parts of bacteria agent;
0.1~0.5 part of octagonal slag;
The octagonal slag is the residue that octagonal fruit or branches and leaves distill out drying after fennel oil;
The bacteria agent is aerobic bacteria and anaerobic bacteria 1:4 weight ratio composition;It is wherein aerobic fungi degradation starch gemma Bacillus;Anaerobic bacteria is lactobacillus acidophilus;
Preparation method includes the following steps:
(1), the pre-processing of filter mud of sugarcane: fresh cane filter mud isolated in sugar refinery 24 hours is removed into moisture, makes it Moisture is no more than 40%;
(2), peat is ground into powdery to 200~400 mesh standard sieves are crossed, then by filter mud of sugarcane, cane molasses, calcium and magnesium phosphorus Fertilizer, rice bran, peat powder potassium fulvate mix in proportion;
(3), it is 25%~40% that adjusting storeroom moisture, which is 25%~40%, C/N ratio,;Adjusting pH is 6~8;
(4), biological bacteria composition is prepared:
Aerobic microbial inoculum and anaerobic bacteria ratio by weight are uniformly mixed;
(5), bio-organic fertilizer is prepared:
The polybag or anaerobic composting tower for charging material into sealing directly cure fermentation, and polybag or anaerobic composting tower are equipped with list To exhaust valve;Whole materials are made all to carry out anaerobic composting fermentation curing in the state of anaerobism, control temperature is lower than 60 DEG C, stacks Time is 25~30 days, is eventually adding plant ash and octagonal slag is uniformly mixed and obtains bio-organic fertilizer.
2. anaerobism adds the method for aerobic fermentation production ecological organic fertilier according to claim 1, which is characterized in that the Xie Dian The bacterial screening of powder series bacillus:
(a), by filter mud of sugarcane stacking or it is put into the cement pit of opening, then uses water spray, makes its wet weight 50~60%, It banks up 20~30 days, stirs daily, it is made to come into full contact with air, rot to filter mud of sugarcane, extract filter mud of sugarcane shower water;
(b), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200~500 mesh;
(c), nutrient agar is culture medium, and P. amylolyticus is first carried out test tube slant culture, is then cultivated again Typical P. amylolyticus, single colonie, the P. amylolyticus list that will be selected are screened after the scribing line culture of ware inclined-plane The bacterium colony nutrient agar that bran powder, octagonal ground-slag, sugarcane marrow powder is added, culture, successively temperature is 35 DEG C, 40 for culture DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 DEG C, filter out 65 DEG C or more of P. amylolyticus;
(d), tame: in nutrient agar add filter mud of sugarcane shower water, then add various concentration calcium magnesium phosphate, Urea steps up the concentration screening domestication of calcium magnesium phosphate, urea, filters out the solution starch gemma for being resistant to higher concentration Bacillus.
3. anaerobism adds the method for aerobic fermentation production ecological organic fertilier according to claim 1, which is characterized in that calcium magnesium phosphate Mass concentration concentration be respectively 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 5%;The mass concentration concentration of urea is respectively 5%、7%、9%、11%、13%、15%、17%、20%。
4. anaerobism adds the method for aerobic fermentation production ecological organic fertilier according to claim 1, which is characterized in that acidophilus cream bar The bacterial screening of bacterium:
(a), inflated with nitrogen before the medium sterilization, it is therefore an objective to remove the oxygen in culture medium, be mentioned for the growth of lactobacillus acidophilus For anaerobic environment;
Then plus water spray (b), filter mud of sugarcane is filled into crock or plastic tank, makes its wet weight 40~50%, sealing makes it It is air tight, place 30 days or more, it rots to filter mud of sugarcane, extracts filter mud of sugarcane shower water;
(c), rice bran, octagonal slag, sugarcane marrow are crushed respectively, powder particle diameter is 200~500 mesh;
(d), nutrient agar is culture medium, and lactobacillus acidophilus is first carried out test tube slant culture, then carries out culture dish inclined-plane again Typical lactobacillus acidophilus is screened after scribing line culture, bran powder, eight is added in the lactobacillus acidophilus single colonie selected by single colonie The nutrient agar of angle ground-slag, sugarcane marrow powder, culture, successively temperature is 35 DEG C, 40 DEG C, 45 DEG C, 55 DEG C, 60 DEG C, 65 for culture DEG C, filter out 65 DEG C or more of lactobacillus acidophilus;
(e), tame: in nutrient agar add filter mud of sugarcane shower water, then add various concentration calcium magnesium phosphate, Urea steps up the concentration screening domestication of calcium magnesium phosphate, urea, filters out the lactobacillus acidophilus for being resistant to higher concentration.
5. anaerobism adds the method for aerobic fermentation production ecological organic fertilier according to claim 1, it is added in raw material mixing micro- 0.01~0.03 part of fertilizer;Micro- fertilizer is containing the compound for being dissolved in the manganese of water, iron, zinc, boron, copper and molybdenum element, element weight Amount ratio is 5:2:1:0.5:0.1:0.01.
6. anaerobism according to claim 1 adds the method for aerobic fermentation production ecological organic fertilier, which is characterized in that the grass Wood ash is that stalk, wormwood, eucalyptus branch, camphor tree branch, pine tree branch, fernwort and/or firewood class burn the plant ash come.
7. anaerobism according to claim 1 adds the method for aerobic fermentation production ecological organic fertilier, which is characterized in that anaerobism heap Fertile tower curing production process is that the material that will be uniformly mixed is sent by conveyer belt to the cone sealed with plastic knitting material to its periphery In shape tower slot, its top is coped with when throwing full with substance and is sealed with plastic knitting material.
8. the bacterial screening of P. amylolyticus as claimed in claim 2, which is characterized in that the nutrient agar Composition:
10~20g of peptone,
Beef extract 3~5g of powder,
10~15g of sodium chloride,
15~20g of agar,
Final pH 7.3 ± 0.2.
9. the bacterial screening of lactobacillus acidophilus as claimed in claim 3, the composition of the nutrient agar:
10~15g of peptone,
Beef extract 3~5g of powder,
10~15g of sodium chloride,
10~15g of agar,
Final pH 7.3 ± 0.2.
CN201810945006.4A 2018-08-20 2018-08-20 Anaerobism adds the method for aerobic fermentation production ecological organic fertilier Pending CN109020688A (en)

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Publication number Priority date Publication date Assignee Title
CN109928849A (en) * 2018-12-20 2019-06-25 青海省湟中县海宁合资化肥厂 A kind of soil amendment fertilizer and the preparation method and application thereof
CN110092698A (en) * 2019-06-24 2019-08-06 珲春三才生物科技有限公司 A kind of biological organic fertilizer and preparation method thereof
CN111919696A (en) * 2020-07-31 2020-11-13 广西壮族自治区林业科学研究院 Method for planting lilium davidii under camellia oleifera forest
CN114751795A (en) * 2022-05-11 2022-07-15 广西大学 Method for preparing sugarcane solid fertilizer by filtering residues from culture tail water

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CN103305451A (en) * 2013-06-20 2013-09-18 双赢集团有限公司 Screening method for ammonium dihydrogen phosphate-tolerant bacillus laterosporus
CN107445711A (en) * 2017-09-15 2017-12-08 黑龙江宝基农业科技股份有限公司 Bio-organic fertilizer made using high calcium selenium-riched vegetable bud and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102172602A (en) * 2011-01-11 2011-09-07 薛松晓 Novel biological treatment method for kitchen garbage
CN102344902A (en) * 2011-08-04 2012-02-08 双赢集团有限公司 Method for screening bacillus subtilis capable of tolerating compound fertilizer
CN103305451A (en) * 2013-06-20 2013-09-18 双赢集团有限公司 Screening method for ammonium dihydrogen phosphate-tolerant bacillus laterosporus
CN107445711A (en) * 2017-09-15 2017-12-08 黑龙江宝基农业科技股份有限公司 Bio-organic fertilizer made using high calcium selenium-riched vegetable bud and preparation method thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109928849A (en) * 2018-12-20 2019-06-25 青海省湟中县海宁合资化肥厂 A kind of soil amendment fertilizer and the preparation method and application thereof
CN110092698A (en) * 2019-06-24 2019-08-06 珲春三才生物科技有限公司 A kind of biological organic fertilizer and preparation method thereof
CN111919696A (en) * 2020-07-31 2020-11-13 广西壮族自治区林业科学研究院 Method for planting lilium davidii under camellia oleifera forest
CN114751795A (en) * 2022-05-11 2022-07-15 广西大学 Method for preparing sugarcane solid fertilizer by filtering residues from culture tail water

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Application publication date: 20181218