CN109007460A - A kind of mixed feed and preparation method thereof improving grass carp immunity - Google Patents
A kind of mixed feed and preparation method thereof improving grass carp immunity Download PDFInfo
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Abstract
The invention discloses a kind of mixed feeds and preparation method thereof for improving grass carp immunity, are made of fish meal, dregs of beans, flour, wheat, soybean oil, rapeseed dregs, cotton dregs, Radix Astragali etc. according to certain weight ratio.Grass compound feed used for fish of the invention, can improve the weight gain and food utilization efficiency of grass carp, and feed coefficient is down to 1.49;Growth of Grass Carps Ctenopharyngodon Idellus can be effectively facilitated, feed of grass carp utilization rate and premunition are improved, there is great practical significance to the sustainable development of grass carp aquaculture.
Description
Technical field
The invention belongs to aquatic feeds technical field more particularly to a kind of promotion Growth of Grass Carps Ctenopharyngodon Idellus and enhance matching for its premunition
Close feed and preparation method thereof.
Background technique
During aquaculture especially fish culture, with intensive degree constantly increase, breeding environment deteriorate and
The outburst of all kinds of diseases is got worse.Meanwhile fish pancreas is its maximum glandula digestive, liver is its most important metabolism device
Official, enteron aisle are its main absorption organs, thus the liver pancreas and enteron aisle of cyprinid fish damage or lesion often lead to its machine
Body metabolic disorders, disease resistance reduce, and easily cause the outburst of secondary disease.Past is in order to be able to satisfy high-density breeding
Under the conditions of optimum growh, while preventing fish disease, it will usually use some chemicals (such as: hormone, antibiotic).And it is long-term
Medication can not only be such that fish self immune system generates abnormal, and the drug resistance of pathogen increases, and the harm of medicament residue
Increasingly show.Therefore, it is imperative to substitute these drugs to seek green, efficient, safety, the feed addictive of noresidue.
Chinese herbal and crude drugs preparations are exactly a kind of green feed additive with bright prospects, it has natural, efficient, poison is secondary to make
With the advantages that small, drug resistance is not significant, resourceful and performance is various, aquatic livestock production performance and feed conversion are being improved
It can fully ensure that the health of human body while rate again, there is medical and trophism double action, be chemical synthesis feed addition
Agent is incomparable.Therefore, a large amount of scholars have carried out the research about Chinese herbal medicine on animal, wherein with Herba Andrographitis and rheum officinale
It is more, the results showed that Herba Andrographitis and rheum officinale have the function of anti-inflammatory sterilization, enhance immune, Hepatoprotective cholagogue, can effectively mitigate liver
It is metabolized damage caused by overload.But Herba Andrographitis and rheum officinale are not embodied in " feedstuff catalogue ".
Summary of the invention
In view of above-mentioned shortcoming, allow matching for the Chinese herbal medicine added containing country the purpose of the present invention is to provide a kind of
Feed is closed, for improving promotion Growth of Grass Carps Ctenopharyngodon Idellus and enhancing its premunition.
In order to achieve the above object, we open corresponding test, and whether the Chinese herb astragalus for determining that country allows to add is right
The Immune-enhancing effect and growth-promoting of grass carp screen its suitable additive amount with certain effect.Specific technical solution is as follows:
A kind of mixed feed improving grass carp immunity, is made of the raw material that following weight matches:
6 parts of fish meal, 43% 22 parts of dregs of beans, 50% 8.5 parts of cottonseed protein, 25 parts of Canadian double lower rapeseed dreg, 12 parts of flour,
13 parts of wheat, 5 parts of maize alcohol lees DDGS, 3 parts of wheat bran, 2.5 parts of soybean oil, 3 parts of premix material additive, Radix Astragali
0.05-0.2 parts.
Further, the mixed feed is made of the raw material that following weight matches:
6 parts of fish meal, 43% 22 parts of dregs of beans, 50% 8.5 parts of cottonseed protein, 25 parts of Canadian double lower rapeseed dreg, 12 parts of flour,
13 parts of wheat, 5 parts of maize alcohol lees DDGS, 3 parts of wheat bran, 2.5 parts of soybean oil, 3 parts of premix material additive, Radix Astragali
0.1 part.
Further, in every kilogram of feed, the premix material additive is grouped as by the group of following weight: P 0.6mg,
Fe 150mg, Cu 3mg, Zn 30.0mg, Mn 13.0mg, Mg 0.05mg, vitamin A 4000.0IU, vitamin D
2000.0IU, vitamin E 100.0mg, vitamin K 10.0mg, vitamin B1 0.5mg, vitamin B2 7.0mg, pantothenic acid
30mg, niacin 28.0mg, vitamin B6 6.0mg, vitamin C 300mg, inositol 400mg, choline 500mg.
Further, the Radix Astragali is that Radix Astragali lateral root or leftover bits and pieces smashing fineness to 90% are sieved with 100 mesh sieve gained.
The present invention also provides a kind of preparation methods of mixed feed for improving grass carp immunity, comprising the following steps:
(1) take fish meal, 43% dregs of beans, 50% cottonseed protein, Canadian double lower rapeseed dreg, flour, wheat, maize alcohol lees DDGS,
Wheat bran is crushed, and smashing fineness reaches 80% and crosses 80 meshes;Then soybean oil is added, premix material additive mixes;
(2) it is eventually adding Radix Astragali mixing, obtains above-mentioned feed.
The present invention adds Chinese herb astragalus in mixed feed, from protecting liver and enteron aisle from damage, growth promotion and mention
In terms of high immunity, the intensive healthy aquaculture of grass carp is ensured.Meanwhile the present invention can improve the weight gain and feed conversion effect of grass carp
Rate, feed coefficient is down to 1.49;Growth of Grass Carps Ctenopharyngodon Idellus can be effectively facilitated, feed of grass carp utilization rate and premunition are improved, grass carp is cultivated
The sustainable development of industry has great practical significance.
Specific embodiment
The present invention will be described combined with specific embodiments below.This test is implemented premised on inventive technique scheme, column
Detailed embodiment and specific operating process out, but protection scope of the present invention is not limited to following specific implementation cases.
Embodiment 1
A kind of mixed feed improving grass carp immunity:
Take fish meal 60kg, 43% dregs of beans 220kg, flour 120kg, wheat 130kg, 50% cottonseed protein 85kg, the double low dishes of Canada
Seed dregs of rice 250kg, 50 kg of maize alcohol lees DDGS, wheat bran 30kg are crushed, and smashing fineness reaches 80% and crosses 80 meshes;So
Soybean oil 25kg, premix material additive 30kg(are added afterwards in every kilogram of mixed feed, and premix material additive is by following weight
Group is grouped as: P 0.6mg, Fe 150mg, Cu 3mg, Zn 30.0mg, Mn 13.0mg, Mg 0.05mg, vitamin A
4000.0IU, vitamin D 2000.0IU, vitamin E 100.0mg, vitamin K 10.0mg, vitamin B1 0.5mg, dimension life
Plain B2 is 7.0mg, pantothenic acid 30mg, niacin 28.0mg, vitamin B6 6.0mg, vitamin C 300mg, inositol 400mg, gallbladder
Alkali 500mg) it mixes;It is eventually adding and resulting Radix Astragali 0.5kg is sieved with 100 mesh sieve by Radix Astragali lateral root or leftover bits and pieces smashing fineness to 90%
It mixes up to the mixed feed of grass carp immunity is improved.
Embodiment 2
A kind of mixed feed improving grass carp immunity:
Take fish meal 60kg, 43% dregs of beans 220kg, flour 120kg, wheat 130kg, 50% cottonseed protein 85kg, the double low dishes of Canada
Seed dregs of rice 250kg, 50 kg of maize alcohol lees DDGS, wheat bran 30kg are crushed, and smashing fineness reaches 80% and crosses 80 meshes;So
Soybean oil 25kg, premix material additive 30kg(are added afterwards in every kilogram of mixed feed, and premix material additive is by following weight
Group is grouped as: P 0.6mg, Fe 150mg, Cu 3mg, Zn 30.0mg, Mn 13.0mg, Mg 0.05mg, vitamin A
4000.0IU, vitamin D 2000.0IU, vitamin E 100.0mg, vitamin K 10.0mg, vitamin B1 0.5mg, dimension life
Plain B2 is 7.0mg, pantothenic acid 30mg, niacin 28.0mg, vitamin B6 6.0mg, vitamin C 300mg, inositol 400mg, gallbladder
Alkali 500mg) it mixes;It is eventually adding and sieves with 100 mesh sieve resulting Radix Astragali 1kg to 90% by Radix Astragali lateral root or leftover bits and pieces smashing fineness and mix
The even mixed feed up to raising grass carp immunity.
Embodiment 3
A kind of mixed feed improving grass carp immunity:
Take fish meal 60kg, 43% dregs of beans 220kg, flour 120kg, wheat 130kg, 50% cottonseed protein 85kg, the double low dishes of Canada
Seed dregs of rice 250kg, 50 kg of maize alcohol lees DDGS, wheat bran 30kg are crushed, and smashing fineness reaches 80% and crosses 80 meshes;So
Soybean oil 25kg, premix material additive 30kg(are added afterwards in every kilogram of mixed feed, and premix material additive is by following weight
Group is grouped as: P 0.6mg, Fe 150mg, Cu 3mg, Zn 30.0mg, Mn 13.0mg, Mg 0.05mg, vitamin A
4000.0IU, vitamin D 2000.0IU, vitamin E 100.0mg, vitamin K 10.0mg, vitamin B1 0.5mg, dimension life
Plain B2 is 7.0mg, pantothenic acid 30mg, niacin 28.0mg, vitamin B6 6.0mg, vitamin C 300mg, inositol 400mg, gallbladder
Alkali 500mg) it mixes;It is eventually adding and sieves with 100 mesh sieve resulting Radix Astragali 2kg to 90% by Radix Astragali lateral root or leftover bits and pieces smashing fineness and mix
The even mixed feed up to raising grass carp immunity.
1. date of test: 15 days-June 13 April in 2012 (first phase), 14 days (second 28 days-June of April in 2017
Phase);1 day-October 15 July in 2017 (third phase).
2. test site: the Sichuan Chengdu Xinjin County Dragon King crosses the test of reservoir Chengdu San Wang agriculture and animal husbandry limited liability company's aquatic products
Base.
3. materials and methods
The test of 3.1 first phases.It is 33% that protein level, which is made, as primary raw material using fish meal, dregs of beans, rapeseed meal, cotton dregs, crude fat
The feed that level is 6%.Test is divided into control group and test group, and every group of 3 repetitions are each to repeat to choose 150kg 250g/ tail
Russian carp.Control group feeds basestocks, test group add on this basis 0.3% compound Chinese herb (Herba Andrographitis: rheum officinale=2:
1).The rate that feeds intake day is 3%-5%, and situation of ingesting, water temperature and Changes in weather according to fish are suitable for adjusting to be fed, and are fed day 4 times
(7:00,11:00,15:00,19:00), with 10 min of potassium permanganate solution dipping of 20 mg/L when fish inlet.Off-test
Blood is acquired at tail bone afterwards, the vigor of serum glutamic oxalacetic transaminase and glutamic-pyruvic transaminase is measured, for evaluating the healthy shape of liver
Condition.Under normal circumstances, liver is its main storage organ, but when damage or necrosis occur for liver, is turned in histocyte
Adnosine deaminase can largely discharge into blood plasma.
The test of 3.2 second phases.The grass carp for choosing 150g/ tail, is randomly divided into three groups, every group of three repetitions, each repetition
78kg.Basestocks per ton are formed by following weight formulated in combination:
Fish meal 60kg, 43% dregs of beans 220kg, flour 120kg, wheat 130kg, 50% cottonseed protein 85kg, the double low vegetable seed of Canada
Dregs of rice 250kg, 50 kg of maize alcohol lees DDGS, wheat bran 30kg, soybean oil 25kg, premix material additive 30kg(are at every kilogram
In basestocks, premix material additive is grouped as by the group of following weight: P 0.6mg, Fe 150mg, Cu 3mg, Zn 30.0mg,
Mn 13.0mg, Mg 0.05mg, vitamin A 4000.0IU, vitamin D 2000.0IU, vitamin E 100.0mg, vitamin K
10.0mg, vitamin B1 0.5mg, vitamin B2 7.0mg, pantothenic acid 30mg, niacin 28.0mg, vitamin B6 6.0mg,
Vitamin C 300mg, inositol 400mg, choline 500mg).Three groups feed basestocks ,+0.1% compound Chinese herb of basestocks respectively
(Herba Andrographitis: rheum officinale=2:1) ,+0.1% Radix Astragali of basestocks a kind of (i.e. raising grass carp immunity obtained by the embodiment of the present invention 2
Mixed feed).The rate that feeds intake day is 3%-5%, and situation of ingesting, water temperature and Changes in weather according to fish are suitable for adjusting to be fed, day
It feeds 4 times (7:00,11:00,15:00,19:00), with 10 min of potassium permanganate solution dipping of 20 mg/L when fish inlet.
After the test, live fish enteron aisle is taken to observe its health status.
The test of 3.3 third phases.The grass carp for choosing 250g/ tail, is randomly divided into five groups, every group of three repetitions, each repetition
100kg.Basestocks per ton are formed by following weight formulated in combination: fish meal 60kg, 43% dregs of beans 220kg, flour 120kg, wheat
130kg, 50% cottonseed protein 85kg, Canadian double lower rapeseed dreg 250kg, 50 kg of maize alcohol lees DDGS, wheat bran 30kg,
In every kilogram of basestocks, premix material additive is grouped by the group of following weight by soybean oil 25kg, premix material additive 30kg(
At: P 0.6mg, Fe 150mg, Cu 3mg, Zn 30.0mg, Mn 13.0mg, Mg 0.05mg, vitamin A 4000.0IU, dimension
Raw element D 2000.0IU, vitamin E 100.0mg, vitamin K 10.0mg, vitamin B1 0.5mg, vitamin B2 are
7.0mg, pantothenic acid 30mg, niacin 28.0mg, vitamin B6 6.0mg, vitamin C 300mg, inositol 400mg, choline
500mg).Five groups feed basestocks (control group), basestocks+0.5Kg/T Radix Astragali (i.e. obtained by the embodiment of the present invention 1 respectively
It is a kind of improve grass carp immunity mixed feed), (i.e. one kind obtained by the embodiment of the present invention 2 mentions basestocks+1Kg/T Radix Astragali
The mixed feed of high grass carp immunity), a kind of basestocks+2Kg/T Radix Astragali (i.e. raising grass carp obtained by the embodiment of the present invention 3
The mixed feed of immunity), basestocks+5Kg/T Radix Astragali.It is as shown in table 1 below to feed ingredient;The rate that feeds intake day is 3%-5%, according to fish
Situation of ingesting, water temperature and Changes in weather be suitable for adjusting to be fed, feed day 4 times (7:00,11:00,15:00,19:00),
With 10 min of potassium permanganate solution dipping of 20 mg/L when fish inlet.After the test, live fish enteron aisle is taken to observe its healthy shape
Condition.
Table 1 feeds list of ingredients
。
4. test result
The test of 4.1 first phases (needs furtherly, Research idea of the invention is " first with the most common Chinese medicine on the market
Whether treatment product adds Chinese medicine effective and feasible (but Herba Andrographitis+rheum officinale is not logged feed register) to verify --- with feed mesh
Available Radix Astragali replaces above-mentioned prescription Herba Andrographitis+rheum officinale in record, it was demonstrated that the suitable use of Radix Astragali also feasible --- --- screening Radix Astragali
Amount ".Meanwhile grass carp is the more maximum Freshwater Fish of China's cultivation amount, although and carp yield slightly be inferior to grass carp, be also one
The main feeding kind of kind, and the disease resistance of carp is better than grass carp.Therefore, if if Chinese medicine can show it effectively on carp
Property, theoretically the application effect on grass carp should be more preferable.)
2 growth performance index of table
。
As shown in Table 2, the growth performance of carp can be improved by Herba Andrographitis+rheum officinale being added in feed, improve its efficiency of feed utilization.
Also, the dead fish of base set is 1.9 times of Herba Andrographitis+rheum officinale group again, shows Chinese herb compound (Herba Andrographitis: the rheum officinale=2:1) energy
Significantly improve the immunity of carp.
The comparison of 3 serum transaminase vigor of table
。
As shown in Table 3, compound Chinese herb (Herba Andrographitis: rheum officinale=2:1) group serum glutamic oxalacetic transaminase vigor significantly reduces, table
The degree of injury of the liver pancreas of bright carp significantly reduces, i.e. the liver pancreas of this Chinese herb compound energy effective protection fish.
The test of 4.2 second phases
The comparison of 4 growth performance of table
。
As shown in Table 4, after grass carp compound feed addition Radix Astragali its growth performance better than base set (being not added with Chinese herbal medicine) and
Herba Andrographitis+rheum officinale group, feed coefficient is minimum, shows that Radix Astragali has growth promotion and improves the remarkable result of efficiency of feed utilization, and excellent
In Herba Andrographitis and rheum officinale.Converted by feed coefficient, Radix Astragali group compared with every feed of base set can how long 90 jin of grass carps, grass carp is at market price
6 yuan/jin are calculated, and feed per ton can bring benefits 540 yuan more.
The comparison of 5 internal organ index of table
。
As shown in Table 5, the coefficient of condition of base set is minimum, shows that this group of fish body condition is poor.Meanwhile the internal organ accounting of Radix Astragali group
It is minimum, show its edible portion ratio highest.The liver pancreas of grass carp is its main digestion and metabolism organ, in high-density breeding
Easily occur the liver and gall syndromes such as fatty liver under environment, to influence its metabolism mesotrophic to feed and reduce its immunity
Energy.In the experiment, two groups of liver body index for adding Chinese herbal medicine is below base set, show add Chinese herbal medicine two groups of grass carps its
The fat content of liver is lower than base set, i.e., the health degree of liver is better than base set, while also reflecting a result: protecting
In terms of the liver pancreas for protecting grass carp, Radix Astragali has the effect of that Herba Andrographitis is similar with rheum officinale.Similarly, enteron aisle disappears as grass carp is important
Change and absorb organ, while being also important immune organ, epithelial tissue is the effective physics screen of blocking harmful substance invasion
Barrier.Spleen is the main immune organ of fish, and endothelial tissue has the same barrier action of epithelial tissue.In the experiment, add
The intestines body index and spleen body index for adding two groups of grass carps of Chinese herbal medicine are below base set, show its intestinal tissue fat content and
The fat content of spleen is lower.And the fat content of epithelium and endothelial tissue is excessively high easily by lipid mistake in the inner in animal body
Oxidation reaction leads to the destruction of institutional framework integrality, reduces the effect of its physical barriers.It can be observed simultaneously by dissection, it is high
Under intensity feeding, the enteron aisle intestinal villus of base set is damaged more serious (casing slime is more before grass carp, and intestinal villus is unintelligible), and Herba Andrographitis+
Rheum officinale group villus is more visible, though the enteron aisle compared with base set grass carp has clear improvement, enteron aisle villus angle is clear, starts at middle intestines
Now a little mucus still shows a degree of damage compared with Radix Astragali group.The entire enteron aisle of Radix Astragali group is clean and villus is long, and performance is most
It is good.Therefore, there should be the structural intergrity of protection grass carp intestinal the result shows that Radix Astragali has Herba Andrographitis and the identical effect of rheum officinale
Effect, to ensure the integrality of its physical barriers function.
The test of 4.3 third phases
The comparison of 6 growth performance of table
。
As shown in Table 6, its growth performance (is not added with Chinese herbal medicine, together better than base set after grass carp compound feed addition Radix Astragali
When, influence of the additive amount of Radix Astragali to growth has differences.When the additive amount of Radix Astragali reaches 1 kg/tonne, feed coefficient is no longer
There is the raising of conspicuousness.Show in this experiment, with optimal cost performance when the additive amount of Radix Astragali is 1 kg/tonne of feed,
The additive amount for improving feed Radix Astragali again can not further significantly improve the growing way of fish.
The comparison of 7 internal organ index of table
。
As shown in Table 7, the coefficient of condition of base set is minimum, shows that this group of grass carp body condition is poor, shows thin.And feed addition Huang
After stilbene, the coefficient of condition of fish can increase, enhance the constitution of fish.After adding Radix Astragali, internal organ ratio has increased trend, but difference is little,
Show have the tendency that increasing grass carp harslet development after increasing Radix Astragali.The liver pancreas of grass carp is its main digestion and metabolism organ, liver
The quality of pancreas development largely decides the quality of fish digestion and metabolic capability.In the experiment, after adding Radix Astragali.Grass
The liver body index of fish has increased trend compared with base set, show add Radix Astragali after grass carp liver pancreas development it is more preferable.Equally
Ground, the enteron aisle digestion and absorption organ important as grass carp, while it being also important immune organ, epithelial tissue is blocking harmful
The effective physical barriers of substance invasion.Spleen is the main immune organ of fish, and endothelial tissue has epithelial tissue same
Barrier action.In the experiment, after feed addition Radix Astragali, the intestines body index and spleen body index of grass carp have compared with control group raised
Trend (enteron aisle and spleen in the experiment have adequately removed adipose tissue), shows its intestinal tissue and Development of The Spleen more
It is good.
Claims (5)
1. a kind of mixed feed for improving grass carp immunity, which is characterized in that it is made of the raw material that following weight matches:
6 parts of fish meal, 43% 22 parts of dregs of beans, 50% 8.5 parts of cottonseed protein, 25 parts of Canadian double lower rapeseed dreg, 12 parts of flour,
13 parts of wheat, 5 parts of maize alcohol lees DDGS, 3 parts of wheat bran, 2.5 parts of soybean oil, 3 parts of premix material additive, Radix Astragali
0.05-0.2 parts.
2. mixed feed according to claim 1, which is characterized in that it is made of the raw material that following weight matches:
6 parts of fish meal, 43% 22 parts of dregs of beans, 50% 8.5 parts of cottonseed protein, 25 parts of Canadian double lower rapeseed dreg, 12 parts of flour,
13 parts of wheat, 5 parts of maize alcohol lees DDGS, 3 parts of wheat bran, 2.5 parts of soybean oil, 3 parts of premix material additive, Radix Astragali
0.1 part.
3. mixed feed according to claim 1 or claim 2, it is characterised in that: in every kilogram of feed, the premix material additive
It is grouped as by the group of following weight: P 0.6mg, Fe 150mg, Cu 3mg, Zn 30.0mg, Mn 13.0mg, Mg 0.05mg, dimension
Raw element A 4000.0IU, vitamin D 2000.0IU, vitamin E 100.0mg, vitamin K 10.0mg, vitamin B1 are
0.5mg, vitamin B2 7.0mg, pantothenic acid 30mg, niacin 28.0mg, vitamin B6 6.0mg, vitamin C 300mg, flesh
Alcohol 400mg, choline 500mg.
4. a kind of mixed feed for improving grass carp immunity according to claim 1 or claim 2, which is characterized in that the Radix Astragali is
Radix Astragali lateral root or leftover bits and pieces smashing fineness to 90% are sieved with 100 mesh sieve into gained.
5. a kind of preparation method of the mixed feed of the raising grass carp immunity as described in claim 1-4 any one, special
Sign the following steps are included:
(1) take fish meal, 43% dregs of beans, 50% cottonseed protein, Canadian double lower rapeseed dreg, flour, wheat, maize alcohol lees DDGS,
Wheat bran is crushed, and smashing fineness reaches 80% and crosses 80 meshes;Then soybean oil is added, premix material additive mixes;
(2) it is eventually adding Radix Astragali mixing, obtains above-mentioned feed.
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