CN109005970B - Method for cuttage of taxus chinensis in summer - Google Patents

Method for cuttage of taxus chinensis in summer Download PDF

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CN109005970B
CN109005970B CN201810838479.4A CN201810838479A CN109005970B CN 109005970 B CN109005970 B CN 109005970B CN 201810838479 A CN201810838479 A CN 201810838479A CN 109005970 B CN109005970 B CN 109005970B
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soil
cutting
summer
cuttage
taxus chinensis
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CN109005970A (en
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杨莉琳
邱发根
李衡
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Tongren University
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Tongren University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/22Improving land use; Improving water use or availability; Controlling erosion
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/40Afforestation or reforestation

Abstract

A method for cutting taxus chinensis in summer comprises the following steps: constructing a nursery greenhouse; treating a soil matrix; fertilizing: when the soil is loaded into a nutrition pot, 4-5g of pure N and P are applied to each kilogram of soil2O52.5‑4g,K2Fertilizing with the proportion of 1.5-2g of O, uniformly mixing with soil and loading into a pot; burying 0.5kg of humus soil in the center of the nutrition pot; collecting cutting shoots; refrigerating and moisturizing; rooting; treating a soil matrix: using high temperature in summer, sterilizing the seedbed soil used as the nutrition pot cuttage in summer for 48-72h, and turning over the soil 1-2 times during the insolation period; the insolated soil is screened by a sieve of 4mm and then is filled into a nutrition pot; cutting; and (5) performing post-management. According to the method, the seedbed soil is subjected to sterilization and disinfection treatment by insolation at high temperature in summer, and base fertilizer is applied, so that the problem that the seedling cultivation and planting technology of the taxus chinensis is limited by seasons is well solved, and the cuttage technology of the taxus chinensis under the high-temperature condition in summer is initiated.

Description

Method for cuttage of taxus chinensis in summer
Technical Field
The invention relates to a method for cuttage of taxus chinensis in summer, and belongs to the technical field of forest planting.
Background
The yew is also named as yew, evergreen arbor, straight trunk and beautiful tree posture, is an excellent potted landscape and greening tree species, and is also a natural anti-cancer plant recognized in the world. The method has a small amount of distribution in Yunnan, Tibet, Heilongjiang, Jilin, Hubei, Sichuan, Guangxi, Jiangxi, Fujian, Guizhou provinces and other provinces in China, and is classified as the first-grade rare or endangered protection tree species in China. The ancient poem "the south China was born with Taxus chinensis and several branches were coming from spring" describes the seeds of Taxus chinensis. The aril is cup-shaped, red, bright and beautiful when it is mature, and can be used for squeezing oil or used as medicine. The Chinese yew has firm and durable wood and beautiful texture, can be carved in high grade, and especially has special efficacy on preventing and treating cancer, leukemia, AIDS, diabetes, cardiovascular diseases and other difficult diseases due to the extraction of taxol from roots, stems, skins and leaves. Therefore, the taxus chinensis is an expensive medicinal tree species and is called as golden tree.
Taxus chinensis is perennial and has the characteristics of shade preference, drought resistance and cold resistance. Therefore, the traditional method selects the cold season of spring and autumn to cultivate the taxus chinensis, and the taxus chinensis is cultivated by a water culture mode of preparing nutrient solution. The Chinese yew seeds have long after-ripening period and dormancy characteristic, can sprout by two winters and one summer under natural conditions, and the seed dormancy needs to be broken by adopting seeding and seedling raising, so the Chinese yew cultured by the seeding method has overlong growth period and has certain requirements on temperature, humidity and soil. Because the soil components are complex and are easy to carry germs, the Chinese yew seedlings are easy to be attacked; in addition, root rot occurs in the taxus chinensis in water culture for a long time. Once the root part of the taxus chinensis is ill, the root part is difficult to repair and recover. Therefore, the taxus chinensis planting technology which is not limited by seasons is urgently needed, the growth and the rapid propagation of the taxus chinensis are promoted, and particularly, the technology for carrying out taxus chinensis cuttage in summer at high temperature is not reported.
Disclosure of Invention
Aiming at the problems of the prior art, the invention provides a method for cuttage of taxus chinensis in summer, which utilizes high temperature in summer to perform sterilization and disinfection treatment on seedbed soil exposure, and applies a base fertilizer, thereby solving the problem that the taxus chinensis planting is limited by seasons, and innovating the cuttage technology of taxus chinensis under the high temperature condition in summer.
The problems stated by the invention are solved by the following technical scheme:
a method for cutting taxus chinensis in summer comprises the following steps:
a. constructing a nursery greenhouse: building a nursery garden and a shading greenhouse before cuttage of the taxus chinensis; selecting sandy loam with good drainage as a nursery, deeply ploughing the soil of the nursery to construct strip platforms with the length of 7-10m, the height of 8-12cm, the width of the upper bottom of 60-80cm and the width of the lower bottom of 70-90cm, and using furrow with the interval of 5-10cm between the strip platforms for drainage; the height of a plastic greenhouse built outside the nursery is 3.2-3.5m, and ventilation and shading are ensured;
b. treating a soil matrix: the soil for cuttage needs to be exposed for 48-72h in summer for sterilization treatment by utilizing the high temperature in summer, and the soil needs to be turned over for 1-2 times during the exposure period; screening the insolated soil by a sieve with the aperture of 4mm, and then filling the soil into a nutrition pot; the depth of the nutrition pot is 28-45cm, the upper caliber is 26-40cm, and the bottom is provided with a water outlet;
c. fertilizing: when the soil is loaded into a nutrition pot, 4-5g of pure N and P are applied to each kilogram of soil2O5 2.5-4g,K2Fertilizing with the proportion of 1.5-2g of O, uniformly mixing with soil and loading into a pot; the soil loading amount of each pot is 5-10kg, the upper edge of the nutrition pot is 5-7cm away from the surface layer of the matrix soil, and after the nutrition pot is loaded, the soil is compacted and is watered thoroughly; burying 0.5kg of humus soil in the center of the nutrition pot;
d. collecting cutting shoots: selecting a strongly growing adult taxus chinensis plant in summer, selecting tender branches at the tail ends of crown branches which are disease-free and well grown and have leaves, wherein fruits are grown on some branches, and cutting the branches into cutting slips with the length of 10-15 cm;
e. refrigerating and moisturizing: immediately putting the cut cutting slips into a No. 10 plastic self-sealing bag filled with clear water, sealing the bag, and putting the bag into a prepared finishing box added with ice blocks; when the treatment cannot be carried out immediately, the mixture is put into a refrigerator at 4 ℃ for refrigeration; ice blocks need to be placed in the storage box during carrying or transportation, and the branch leaves are kept from being damaged by extrusion;
f. rooting: the rooting powder is prepared by the following steps: water is 2-2.5: 1000, soaking the lower part of the cutting in a rooting powder solution for 8-10 hours, wherein the length of the lower part of the cutting is 5-8 cm;
g. cuttage: cutting the taxus chinensis cutting into the soil of a nutrition pot for 5-8cm within 1-2h after the soil is soaked in water, and exposing the top end of the cutting to the ground for 5-7cm after cutting;
h. and (4) post management: placing the well-cut Chinese yew nutrition pot on the nursery soil in the shading greenhouse so as to exchange water and nutrients with the soil, placing the nutrition pot in a non-suspended manner, keeping the water content of the nursery soil at 23-26%, watering once every day, and watering for 9 am: 00-10: 00; watering every 1 day after 6 weeks of cuttage; after autumn equinox-before the next year of grain rain, water is poured every 4 days; watering once every 2 days after the grain rain along with the rise of the air temperature;
i. seed maturation: during cuttage, the Chinese yew seeds of the cutting slips with fruits are mature successively at the beginning of 11-12 months, and the cutting slips are picked and harvested in time;
j. and (4) outplanting: after the cuttings survive for 2 years, the main stem can grow to more than 50cm, the number of the tillers reaches 6-8, and the cuttings can be outplanted for forestation; the tillers with good growth vigor can also be selected to be propagated by a cutting method.
According to the method for cuttage of the taxus chinensis in summer, the nursery soil surface in the step a and the nutrition pot soil surface in the step b are sporadically covered with cobblestones with the diameter of 5-7 cm.
In the method for cuttage of taxus chinensis in summer, in the step f, the rooting powder comprises indole, naphthylacetic acid and 2, 4 nitre auxiliary agent.
In the method for cuttage of the taxus chinensis in summer, in the steps c and h, the watering amount is guaranteed to be 10-15cm through the soil layer.
In the method for cuttage of the taxus chinensis in summer, in the step g, 5-8 small cobblestones with the diameter of 5-7cm are placed around the cutting slips.
The invention discloses a method for cutting taxus chinensis in summer aiming at the growth characteristics of the taxus chinensis. According to the method, sandy loam with good drainage is selected to serve as a nursery garden, deep ploughing and fine cropping are carried out on seedbed soil, the seedbed soil is exposed to the sun at high temperature in summer for sterilization and disinfection, and base fertilizer is applied.
Drawings
FIG. 1 is a diagram of the growth condition of the yew cut by the method of the invention.
Detailed Description
Several embodiments of the invention are provided below:
the following examples are experimental examples of the method of the present invention in university student's scientific park in the belly of wuling mountain. The area is located in the Guizhou province copper kernel city and belongs to a karst landform development typical area. The climate belongs to a subtropical monsoon humid climate region, and the climate characteristics are mainly shown in that the monsoon climate is obvious and the vertical difference of the climate is obvious. The annual sunshine duration is 1044.7-1266.2h, the annual average temperature is 13.5-17.6 ℃, the daily average temperature is more than 10 ℃, the initial day is at the beginning of 3 late ten days, the final day is at the beginning of 11 late ten days, the interval is 250d, and the accumulated temperature is 5300 ℃. The average annual precipitation is 1110-1410mm, the frost-free period is 275-317d, the heat is abundant, the illumination is proper, and the precipitation is abundant.
Example 1
Constructing a nursery greenhouse: building a nursery garden and a shading greenhouse before cuttage of the taxus chinensis; selecting sandy loam with good drainage as a nursery, deeply ploughing the soil of the nursery to construct strip platforms with the length of 7-10m, the height of 8-12cm, the width of the upper bottom of 60-80cm and the width of the lower bottom of 70-90cm, and using furrow with the interval of 5-10cm between the strip platforms for drainage; covering the surface of nursery soil with cobblestones with the diameter of 5-7cm so as to drain water and reduce water evaporation; the height of a plastic greenhouse built outside the nursery is 3.2-3.5m, and ventilation and shading are ensured;
treating a soil matrix: the soil for cuttage needs to be exposed for 48-72h in summer for sterilization treatment by utilizing the high temperature in summer, and the soil needs to be turned over for 1-2 times during the exposure period; screening the insolated soil by a sieve with the aperture of 4mm, and then filling the soil into a nutrition pot; the depth of the nutrition pot is 28-45cm, the upper caliber is 26-40cm, and the bottom is provided with a water outlet;
fertilizing: when the soil is loaded into a nutrition pot, pure N4 g and P are applied to each kilogram of soil2O5 2.5g,K2Fertilizing with the proportion of O1.5 g, uniformly mixing with soil and loading into a pot; the soil loading amount of each pot is 5-10kg, the upper edge of the nutrition pot is 5-7cm away from the surface layer of the matrix soil, and after the nutrition pot is loaded, the soil is compacted and is watered thoroughly; burying 0.5kg of humus soil in the center of the nutrition pot;
collecting cutting shoots: selecting a robust growing adult taxus chinensis plant in summer, selecting a tender branch at the tail end of a crown branch which is disease-free, well-growing and provided with leaves, and cutting the branch into a cutting shoot with the length of 10-15 cm; refrigerating and moisturizing: immediately putting the cut cutting slips into a No. 10 plastic self-sealing bag filled with clear water, sealing the bag, and putting the bag into a prepared finishing box added with ice blocks; when the treatment cannot be carried out immediately, the mixture is put into a refrigerator at 4 ℃ for refrigeration; ice blocks need to be placed in the storage box during carrying or transportation, and the branch leaves are kept from being damaged by extrusion;
rooting: the green Dior rooting powder is prepared by the following steps: the water content is 2: 1000, soaking the lower part of the cutting in a rooting powder solution for 8-10 hours, wherein the length of the lower part of the cutting is 5-8 cm;
cuttage: cutting the taxus chinensis cutting into the soil of a nutrition pot for 5-8cm within 1-2h after the soil is soaked in water, and exposing the top end of the cutting to the ground for 5-7cm after cutting; 5-8 small cobblestones with the diameter of 5-7cm are placed around the cuttings to play a role in covering;
and (4) post management: placing the well-cut Chinese yew nutrition pot on the nursery soil in the shading greenhouse so as to exchange water and nutrients with the soil, placing the nutrition pot in a non-suspended manner, keeping the water content of the nursery soil at 23-26%, watering once every day, and watering for 9 am: 00-10: 00; watering every 1 day after 6 weeks of cuttage; after autumn equinox-before the next year of grain rain, water is poured every 4 days; watering once every 2 days after the grain rain along with the rise of the air temperature, wherein the watering amount of each time ensures that the soil layer is soaked by 10-15 cm; seed maturation: during cuttage, the Chinese yew seeds of the cutting slips with fruits are mature successively at the beginning of 11-12 months, and the cutting slips are picked and harvested in time;
and (4) outplanting: after the cuttings survive for 2 years, the main stem can grow to more than 50cm, the number of the tillers reaches 6-8, and the cuttings can be outplanted for forestation; the tillers with good growth vigor can also be selected to be propagated by a cutting method.
Example 2
Fertilizing: when the soil is loaded into a nutrition pot, pure N5 g and P are applied to each kilogram of soil2O5 4g,K2Fertilizing the O2 g in proportion, uniformly mixing the fertilizer with soil and loading the mixture into a pot; the soil loading amount of each pot is 5-10kg, the upper edge of the nutrition pot is 5-7cm away from the surface layer of the matrix soil, and after the nutrition pot is loaded, the soil is compacted and is watered thoroughly; burying 0.5kg of humus soil in the center of the nutrition pot; the green Dior rooting powder is prepared by the following steps: water 2.5: 1000, soaking the lower part of the cutting in a rooting powder solution for 8-10 hours, wherein the length of the lower part of the cutting is 5-8 cm; the other procedures were the same as in example 1.
Example 3
Fertilizing: when the soil is loaded into a nutrition pot, 4.3g of pure N and P are applied to each kilogram of soil2O5 3g,K2Fertilizing with the proportion of O1.5 g, uniformly mixing with soil and loading into a pot; the soil loading amount of each pot is 5-10kg, the upper edge of the nutrition pot is 5-7cm away from the surface layer of the matrix soil, and after the nutrition pot is loaded, the soil is compacted and is watered thoroughly; burying 0.5kg of humus soil in the center of the nutrition pot; the green Dior rooting powder is prepared by the following steps: the water content is 2.2: 1000 parts by weightForming a solution, and soaking the lower part of the cutting strip with the length of 5-8cm in the rooting powder solution for 8-10 h; the other procedures were the same as in example 1.
Example 4
Fertilizing: when the soil is loaded into a nutrition pot, 4.6g of pure N and P are applied to each kilogram of soil2O5 3.5g,K2Fertilizing with the proportion of O1.7 g, uniformly mixing with soil and loading into a pot; the soil loading amount of each pot is 5-10kg, the upper edge of the nutrition pot is 5-7cm away from the surface layer of the matrix soil, and after the nutrition pot is loaded, the soil is compacted and is watered thoroughly; burying 0.5kg of humus soil in the center of the nutrition pot; the green Dior rooting powder is prepared by the following steps: water 2.4: 1000, soaking the lower part of the cutting in a rooting powder solution for 8-10 hours, wherein the length of the lower part of the cutting is 5-8 cm; the other procedures were the same as in example 1.
Referring to table 1, the method of the invention enables the physicochemical properties of the matrix soil to meet the nutritional conditions required by cutting taxus chinensis by applying the base fertilizer. The survival rate of the yew cut by the method reaches 85% or more, and the technology for cutting the yew under the high-temperature condition in summer is innovated.
TABLE 1 conditions to be satisfied by the physicochemical properties of the substrate soil for cutting Taxus chinensis
pH Nitrate nitrogen (mg/kg) Ammonium nitrogen (mg/kg) Total nitrogen (g/kg) Organic matter (%) Available phosphorus (mg/kg) Is effectivePotassium (mg/kg)
3.97-6.9 2.1-15.8 3.8-25.2 2.4-6.7 4.5-8.2 10.2-15 180-230
Referring to fig. 1, the yew cut by the method of the invention is in rooting period within 5 months after potting, overground part grows very slowly, and the growth is accelerated 10 months after potting. The plant height of the taxus chinensis rises exponentially.

Claims (4)

1. A method for cutting taxus chinensis in summer is characterized in that the method for cutting taxus chinensis in high temperature condition in summer comprises the following steps:
a. constructing a nursery greenhouse: building a nursery garden and a shading greenhouse before cuttage of the taxus chinensis; selecting sandy loam with good drainage as a nursery, deeply ploughing the soil of the nursery to construct strip platforms with the length of 7-10m, the height of 8-12cm, the width of the upper bottom of 60-80cm and the width of the lower bottom of 70-90cm, and using furrow with the interval of 5-10cm between the strip platforms for drainage; the height of a plastic greenhouse built outside the nursery is 3.2-3.5m, and ventilation and shading are ensured;
b. treating a soil matrix: sterilizing the soil for cuttage in summer by using high temperature in summer for 48-72h, wherein the soil needs to be turned over for 1-2 times during the exposure period; screening the insolated soil by a sieve with the aperture of 4mm, and then filling the soil into a nutrition pot; the depth of the nutrition pot is 28-45cm, the upper caliber is 26-40cm, and the bottom is provided with a water outlet;
c. fertilizing: when the soil is loaded into a nutrition pot, 4-5g of pure N and P are applied to each kilogram of soil2O5 2.5-4g,K2Fertilizing with the proportion of 1.5-2g of O, uniformly mixing with soil and loading into a pot; the soil loading amount of each pot is 5-10kg, the distance between the upper edge of the nutrition pot and the surface layer of the matrix soil is 5-7cm, and the soil is loadedCompacting soil, and thoroughly watering; burying 0.5kg of humus soil in the center of the nutrition pot;
d. collecting cutting shoots: selecting a robust growing adult taxus chinensis plant in summer, selecting a tender branch at the tail end of a crown branch which is disease-free, well-growing and provided with leaves, and cutting the branch into a cutting shoot with the length of 10-15 cm;
e. refrigerating and moisturizing: immediately putting the cut cutting slips into a No. 10 plastic self-sealing bag filled with clear water, sealing the bag, and putting the bag into a prepared finishing box added with ice blocks; when the treatment cannot be carried out immediately, the mixture is put into a refrigerator at 4 ℃ for refrigeration; ice blocks need to be placed in the storage box during transportation, and the branch leaves are kept from being damaged by extrusion;
f. rooting: the rooting powder is prepared by the following steps: water is 2-2.5: 1000, soaking the lower part of the cutting slips 5-8cm in the rooting powder solution for 8-10 h;
g. cuttage: cutting the taxus chinensis cutting into the soil of a nutrition pot for 5-8cm within 1-2h after the soil is soaked in water, and exposing the top end of the cutting to the ground for 5-7cm after cutting;
h. and (4) post management: placing the well-cut Chinese yew nutrition pot on the nursery soil in the shading greenhouse so as to exchange water and nutrients with the soil, placing the nutrition pot in a non-suspended manner, keeping the water content of the nursery soil at 23-26%, watering once every day, and watering for 9 am: 00-10: 00; watering every 1 day after 6 weeks of cuttage; after autumn equinox-before the next year of grain rain, water is poured every 4 days; watering once every 2 days after the grain rain along with the rise of the air temperature;
i. seed maturation: during cuttage, branch cuttings with fruits have taxus seeds which are mature successively at the beginning of 11-12 months, and the taxus seeds are picked and harvested in time;
j. and (4) outplanting: after the cutting shoots survive for 2 years, the main stems can grow to be more than 50cm, the number of the tillers reaches 6-8, and the cutting shoots can be outplanted for forestation; the tillers with good growth vigor can also be selected to be propagated by a cutting method.
2. The method for summer cuttage of taxus chinensis according to claim 1, wherein: and c, sporadically covering small cobblestones with the diameter of 5-7cm on the nursery soil surface in the step a and the soil surface of the nutrition pot in the step b.
3. The method for summer cuttage of taxus chinensis according to claim 1, wherein: and c, in the steps h, the watering amount is ensured to be 10-15cm through the soil layer.
4. The method for summer cuttage of taxus chinensis according to claim 1, wherein: and g, placing 5-8 small cobblestones with the diameter of 5-7cm around the cuttings.
CN201810838479.4A 2018-07-27 2018-07-27 Method for cuttage of taxus chinensis in summer Active CN109005970B (en)

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CN101982050B (en) * 2010-09-30 2011-09-21 哈尔滨红豆杉科技开发有限责任公司 Method for asexually propagating taxus cuspidata
CN102893788B (en) * 2012-10-23 2014-05-28 黄山市黟县环宇农业科技有限责任公司 Method for cuttage of taxus chinensis
CN103109665A (en) * 2013-02-25 2013-05-22 江西省喜果绿化有限公司 Cutting propagation method for Chinese yew by seedling pots
CN103283460B (en) * 2013-06-07 2014-12-10 江苏红豆杉生物科技股份有限公司 Taxus media cuttage method
CN103563638A (en) * 2013-11-15 2014-02-12 黄山市黟县环宇农业科技有限责任公司 Taxus chinensis var mairei artificial propagation technology

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