CN101982050B - Method for asexually propagating taxus cuspidata - Google Patents

Method for asexually propagating taxus cuspidata Download PDF

Info

Publication number
CN101982050B
CN101982050B CN201010298148XA CN201010298148A CN101982050B CN 101982050 B CN101982050 B CN 101982050B CN 201010298148X A CN201010298148X A CN 201010298148XA CN 201010298148 A CN201010298148 A CN 201010298148A CN 101982050 B CN101982050 B CN 101982050B
Authority
CN
China
Prior art keywords
northeast
taxus chinensis
cuttage
seedling
year
Prior art date
Application number
CN201010298148XA
Other languages
Chinese (zh)
Other versions
CN101982050A (en
Inventor
王志国
齐桂芳
Original Assignee
哈尔滨红豆杉科技开发有限责任公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 哈尔滨红豆杉科技开发有限责任公司 filed Critical 哈尔滨红豆杉科技开发有限责任公司
Priority to CN201010298148XA priority Critical patent/CN101982050B/en
Publication of CN101982050A publication Critical patent/CN101982050A/en
Application granted granted Critical
Publication of CN101982050B publication Critical patent/CN101982050B/en

Links

Abstract

The invention provides a method for asexually propagating taxus cuspidata, relating to the method for propagating the taxus cuspidate. The invention solves the problems of low rooting rate and weak nursery stock in the existing cutting propagation method for the taxus cuspidate and low survival rate under natural condition when the taxus cuspidata is transplanted for afforestation on a mountain. The method comprises the following steps: ears are picked up in winter of the first year, and is buried by ice and snow; in spring of the second year, cutting is carried out on a disinfected cutting bed, a film is coated on the cutting bed in 2.5 to 3 months with humidity of between 85 and 88 percent at the temperature of between 26 and 29 DEG C and shading degree of between 70 and 75 percent after cutting is completed, and the cutting bet is subject to fertilizer application and sterilization; the coated film is removed, cold-proof water is irrigated before winter; and the bed is changed in spring of the third year, and culture is conducted for two years to obtain the taxus cuspidata for afforestation. The cultured sapling has 100 percent of lignification, 80 to 96 percent of cut rooting rate and 85 to 90 percent of planting percentage, thus the mature period of medical forest is shortened. The method can be used for large-scale afforestation.

Description

The method of taxus chinensis in northeast pierre
Technical field
The present invention relates to the method that taxus chinensis in northeast is bred.
Background technology
Taxus chinensis in northeast has another name called Japanese yew, it is taxaceae magaphanerophytes, be rare tree species, its stem, branch, leaf, root all can be used as medicine, main component is taxol, taxine, diterpenoids compound, have anti-cancer function is arranged, and the diabetes of inhibition are arranged and treat cardiopathic effectiveness, seed can also extract oil in addition.Because taxus chinensis in northeast is medicinal seeds in imminent danger, dioecism, and it is fragmentary to distribute is difficult to collect seed and seeding ratio is low, is difficult to satisfy breed needs on a large scale; Publication number is the cultivation method that the Chinese patent of CN1745619A discloses a kind of Chinese yew, this method is at solar term in clear and bright and the beginning of autumn, plant branching is cut, be trimmed to 10cm~20cm and become section, the bottom leaf is removed, put into and carry out cuttage after the soup of taking root soaks 20min~40min, cuttage and seedling culture soil is made up of foaming perlite, vermiculite, soil and sand equal portions batching, cuttage was taken root in 3~3.5 months, move into then and cultivate 3.5~4.5 months in the nutritive cube, long to sapling to 10cm~20cm, cut the earth and transplant, carry out Routine Management then.The nursery stock that this method is cultivated is thin and delicate, malnutrition, and lignification is bad, is unfavorable for seedling overwintering.Publication number is the breeding and seedling method that the Chinese patent of CN1711826A discloses a kind of Chinese yew, and this method is cut into segment with the first tender shoots of Chinese yew spring, and every section has 2~4 true leaf buds; Then with Chinese yew just the tender shoots segment plant in the breeding humus soil is grown seedlings base, insulation urging grow seedlings germinate to the bud strain be more than 12 centimetres; Again segment is cut in the bud strain, every section 2~4 true leaf buds are planted in the breeding humus soil and are grown seedlings in the base, insulation urging grow seedlings germinate to the bud strain be more than the 12cm; Treat that the bud strain decides to transplant after the strain.This method is bred with the first tender shoots of Chinese yew, and the Chinese yew seedling that obtains is thin and delicate, transplants on the Yushan Hill and afforests, and survival rate is 40%~60% only under field conditions (factors), and degree of lignification is low, and cold resistance is poor, and technology is loaded down with trivial details, and production cost is than higher.
Summary of the invention
The present invention be low for the cuttage breeding method rooting rate that solves existing Chinese yew, nursery stock is thin and delicate, transplant on the Yushan Hill and afforest, the low problem of survival rate under field conditions (factors), and the method for taxus chinensis in northeast pierre is provided.
The method of taxus chinensis in northeast pierre of the present invention is carried out according to the following steps: one, select fringe: the winter in 1 year, the middle and upper part of Chinese yew tree crown was northeastward cut and is adopted 1~3 year whole lignified branch of life and make cuttage bar fringe, buries stand-by with ice and snow; Two, cutting bed was made: the spring in 1 year, by the mass ratio of detritus soil and river sand is that 1:1.4~1.6 take by weighing detritus soil and river sand and mix, carry out disinfection, after desinsection handles, being made into width in the greenhouse or on the open country is that 0.9m~1.2m, thickness are the cutting bed of 0.25m~0.30m; Three, the skewer fringe is handled: it is 10cm~15cm that the bar fringe of step 1 collection is cut into length, and the plugged ear of base diameter 〉=0.2cm, plugged ear have 1 terminal bud or short lateral bud, use HORMONE TREATMENT then; Four, cuttage: with mass percent concentration is that 0.18%~0.22% liquor potassic permanganate sprays cutting bed, every square metre is sprayed 2L~3L, the plugged ear that step 3 is handled is vertically inserted on the cutting bed then, the cuttage degree of depth is 1/3 of a cutting length, the line space of cuttage is from being 5cm~6cm, and distance is 3cm~3.5cm between the strain in every row, is 0.09%~0.11% liquor potassic permanganate sprinkling immediately with mass percent concentration after the cuttage, every square metre is sprayed 2L~3L, and then washes leaf with clear water; Five, cuttage seeding management: from cuttage finish to after 2.5~3 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%~88%, temperature is 26 ℃~29 ℃, obscurity is 70%~75%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once sprayed fertilizer once in per ten days, and wherein the trace element fertilizer amount of application is 0.03g/m 2~0.04g/m 2, the fertile amount of application of macroelement is 5g/m 2~10g/m 2, remove plastic film on the cutting bed then, the relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%~88%, obscurity is 70%~75%; Six, overwinter management: in the greenhouse, grow seedlings, when the temperature in the greenhouse is low to moderate 4 ℃~6 ℃, irrigate winter protection water, keeping indoor temperature then is-10 ℃~-35 ℃, and obscurity is the 70%~75%, three year spring, when temperature was stablized by 4 ℃~6 ℃, adjusting obscurity was 50%~60%; On open country, grow seedlings, when the minimum air temperature is low to moderate 11 ℃, the taxus chinensis in northeast seedling is covered, cover the straw screen or mat shading on the plastic foil with plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating in the 3rd year spring, removes straw screen or mat when temperature is stablized by 10 ℃; Seven, changing bed cultivates: the 3rd year spring, by the mass ratio of detritus soil and river sand is that 1:1.4~1.6 take by weighing detritus soil and river sand and mix, carry out disinfection, after desinsection is handled, being made into width on open country is 0.9m~1.1m, thickness is the seedbed of 0.25m~0.30m, then when temperature is stablized by 15 ℃, the taxus chinensis in northeast seedling that step 6 is cultivated is pressed line space from being 10cm~11cm, distance is implanted on the seedbed for 5cm~6cm between strain, cultivated 2 years, in biennium, spring, keep that relative moisture is 85%~88% in the growing space of Chinese yew seedling in summer and autumn, obscurity is 70%~75%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once, sprayed fertilizer once in per ten days, wherein the amount of application of trace element fertilizer is 0.03g~0.04g/m 2, the amount of application of macroelement fertilizer is 5g/m 2~10g/m 2With plastic foil cover taxus chinensis in northeast seedling when being low to moderate 11 ℃ when the minimum air temperature autumn, covers the straw screen or mat shading on the plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating; The taxus chinensis in northeast seedling of afforestation obtains going up a hill; Wherein the HORMONE TREATMENT described in the step 3 is meant with skewer fringe root concentration to be that the α-Nai Yisuan of 40ppm~100ppm is soaked 15h~24h or is 40 * 10 with skewer fringe root concentration -6μ L/L~70 * 10 -6Soaking with indole butyric acid 10h~14h of μ L/L.
The present invention gathered cuttage bar fringe 1 year winter, buried with ice and snow, kept the bar fringe to be in the state of not sprouting, and nutrition is not run off, and prolong the activity duration, and ice and snow buried and can sterilize.By HORMONE TREATMENT to plugged ear, the temperature of cuttage seeding, humidity, illumination and fertile management, make the nutrition of taxus chinensis in northeast seedling abundant, nursery stock is healthy and strong, 100% lignification, method of the present invention makes taxus chinensis in northeast cuttage root-taking rate reach 80%~96%, planting percent reaches 85%~90%, accelerate the speed of seedling-wood breeding, the illumination of this method, temperature, damp condition is fit to the taxus chinensis in northeast growth, make the high amount of growth of taxus chinensis in northeast year of artificial breeding reach 10cm~25cm, and the about 5cm~8cm of high amount of growth of natural taxus chinensis in northeast year, make the taxus chinensis in northeast afforestation of artificial culture get final product collection and utilization after 3 years, under the situation that does not reduce the medicinal content of taxus chinensis in northeast taxol, shortened the technical maturity of the medicinal woods of taxus chinensis in northeast; The clone taxus chinensis in northeast of artificial culture is the shrub shape more, be thick with leaves, amount of growth is big, after measured, its branches and leaves dry matter biomass yield is more than 2.5 times of natural taxus chinensis in northeast of the same age: natural living branches and leaves (dry matter) are 0.1 kilogram/strain~0.2 kilogram/strain, artificial culture be 0.3 kilogram/strain~0.5 kilogram/strain; The taxus chinensis in northeast of artificial culture was cut every 1 year and is gathered branches and leaves once, and increased with the age of tree, and its branches and leaves output increases progressively, and has guaranteed the amount of growth and the Sustainable utilization of resources of the medicinal woods maximum of Chinese yew.
Embodiment
Embodiment one: the method for the taxus chinensis in northeast pierre of present embodiment was carried out according to the following steps: one, select fringe: the winter in 1 year, the middle and upper part of Chinese yew tree crown northeastward, cut and adopt 1 year~3 years whole lignified branches of life and make cuttage bar fringe, bury stand-by with ice and snow; Two, cutting bed was made: the spring in 1 year, by the mass ratio of detritus soil and river sand is that 1:1.4~1.6 take by weighing detritus soil and river sand and mix, carry out disinfection, after desinsection handles, being made into width in the greenhouse or on the open country is that 0.9m~1.2m, thickness are the cutting bed of 0.25m~0.30m; Three, the skewer fringe is handled: it is 10cm~15cm that the bar fringe of step 1 collection is cut into length, and the plugged ear of base diameter 〉=0.2cm, plugged ear have 1 terminal bud or short lateral bud, use HORMONE TREATMENT then; Four, cuttage: with concentration is 0.18%~0.22%(quality) liquor potassic permanganate spray cutting bed, every square metre is sprayed 2L~3L, the plugged ear that step 3 is handled is vertically inserted on the cutting bed then, the cuttage degree of depth is 1/3 of a cutting length, the line space of cuttage is from being 5cm~6cm, and distance be 3cm~3.5cm between the strain in every row, uses 0.09%~0.11%(quality after the cuttage immediately) the liquor potassic permanganate sprinkling, every square metre is sprayed 2L~3L, and then washes leaf with clear water; Five, cuttage seeding management: from cuttage finish to after 2.5~3 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%~88%, temperature is 26 ℃~29 ℃, obscurity is 70%~75%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once sprayed fertilizer once in per ten days, and wherein the trace element fertilizer amount of application is 0.03g/m 2~0.04g/m 2, the fertile amount of application of macroelement is 5g/m 2~10g/m 2, remove plastic film on the cutting bed then, the relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%~88%, obscurity is 70%~75%; Six, overwinter management: in the greenhouse, grow seedlings, when the temperature in the greenhouse is low to moderate 4 ℃~6 ℃, irrigate winter protection water, keeping indoor temperature then is-10 ℃~-35 ℃, and obscurity is the 70%~75%, three year spring, when temperature was stablized by 4 ℃~6 ℃, adjusting obscurity was 50%~60%; On open country, grow seedlings, when the minimum air temperature is low to moderate 9 ℃~11 ℃, the taxus chinensis in northeast seedling is covered, cover the straw screen or mat shading on the plastic foil with plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating in the 3rd year spring, removes straw screen or mat when temperature is stablized by 10 ℃; Seven, changing bed cultivates: the 3rd year spring, by the mass ratio of detritus soil and river sand is that 1:1.4~1.6 take by weighing detritus soil and river sand and mix, carry out disinfection, after desinsection is handled, being made into width on open country is 0.9m~1.1m, thickness is the seedbed of 0.25m~0.30m, then when temperature is stablized by 15 ℃, the taxus chinensis in northeast seedling that step 6 is cultivated is pressed line space from being 10cm~11cm, distance is implanted on the seedbed for 5cm~6cm between strain, cultivated 2 years, in biennium, spring, keep that relative moisture is 85%~88% in the growing space of Chinese yew seedling in summer and autumn, obscurity is 70%~75%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once, sprayed fertilizer once in per ten days, wherein the amount of application of trace element fertilizer is 0.03g~0.04g/m 2, the amount of application of macroelement fertilizer is 5g/m 2~10g/m 2With plastic foil cover taxus chinensis in northeast seedling when being low to moderate 9 ℃~11 ℃ when the minimum air temperature autumn, covers the straw screen or mat shading on the plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating; The taxus chinensis in northeast seedling of afforestation obtains going up a hill; Wherein the HORMONE TREATMENT described in the step 3 is meant with skewer fringe root concentration to be that the α-Nai Yisuan of 40ppm~100ppm is soaked 15h~24h or is 40 * 10 with skewer fringe root concentration -6μ L/L~70 * 10 -6Soaking with indole butyric acid 10h~14h of μ L/L.
Trace element fertilizer in the present embodiment step 5 and macroelement fertilizer are the commercially available prod.
Present embodiment was gathered cuttage bar fringe 1 year winter, buried with ice and snow, kept the bar fringe to be in the state of not sprouting, and nutrition is not run off, and prolong the activity duration, and ice and snow buried and can sterilize.By HORMONE TREATMENT to plugged ear, the temperature of cuttage seeding, humidity, illumination and fertile management, make the nutrition of taxus chinensis in northeast seedling abundant, nursery stock is healthy and strong, cultured sapling 100% lignification, the method of present embodiment makes taxus chinensis in northeast cuttage root-taking rate reach 80%~96%, planting percent reaches 85%~90%, accelerate the speed of seedling-wood breeding, the illumination of this method, temperature, damp condition is fit to the taxus chinensis in northeast growth, make the high amount of growth of taxus chinensis in northeast year of artificial breeding reach 10cm~25cm, and about 5 cm of high amount of growth of natural taxus chinensis in northeast year~8cm, make the taxus chinensis in northeast afforestation of artificial culture get final product collection and utilization after 3 years, under the situation that does not reduce the medicinal content of taxus chinensis in northeast taxol, shortened the technical maturity of the medicinal woods of taxus chinensis in northeast; The clone taxus chinensis in northeast of artificial culture is the shrub shape more, be thick with leaves, amount of growth is big, after measured, its branches and leaves dry matter biomass yield is more than 2.5 times of natural taxus chinensis in northeast of the same age: natural living branches and leaves (dry matter) are 0.1 kilogram/strain~0.2 kilogram/strain, artificial culture be 0.3 kilogram/strain~0.5 kilogram/strain; The taxus chinensis in northeast of artificial culture was cut every 1 year and is gathered branches and leaves once, and increased with the age of tree, and its branches and leaves output increases progressively, and has guaranteed the amount of growth and the Sustainable utilization of resources of the medicinal woods maximum of Chinese yew.
Embodiment two: what present embodiment and embodiment one were different is: the river sand diameter 1mm~0.1mm described in the step 2.Other is identical with embodiment one.
Embodiment three: what present embodiment and embodiment one were different is: the river sand diameter 0.8mm~0.3mm described in the step 2.Other is identical with embodiment one.
Embodiment four: what present embodiment was different with one of embodiment one to three is: the sterilization described in step 2 and the step 7, desinsection are handled and carried out according to the following steps: 500 times of liquid with carbendazim 50% wetting powder spray soil, and every square metre is 2L~3L with the amount of spraying.Other is identical with one of embodiment one to three.
Embodiment five: what present embodiment was different with one of embodiment one to three is: the sterilization described in step 2 and the step 7, desinsection are handled and carried out according to the following steps: 500 times of liquid with carbendazim 50% wetting powder spray soil, and every square metre is 2.5L with the amount of spraying.Other is identical with one of embodiment one to three.
Embodiment six: what present embodiment was different with one of embodiment one to five is: cut in the step 1 and adopt 2 years whole lignified branches of life and make cuttage bar fringe.Other is identical with one of embodiment one to five.
Embodiment seven: what present embodiment was different with one of embodiment one to six is: the mass ratio of detritus soil and river sand is 1:1.45~1.55 in the step 2.Other is identical with one of embodiment one to six.
Embodiment eight: what present embodiment was different with one of embodiment one to six is: the mass ratio of detritus soil and river sand is 1:1.5 in the step 2.Other is identical with one of embodiment one to six.
Embodiment nine: what present embodiment was different with one of embodiment one to eight is: the width of cutting bed is that 0.95m~1.15m, thickness are 0.26m~0.28m in the step 2.Other is identical with one of embodiment one to eight.
Embodiment ten: what present embodiment was different with one of embodiment one to eight is: the width of cutting bed is that 1.1m, thickness are 0.27m in the step 2.Other is identical with one of embodiment one to eight.
Embodiment 11: what present embodiment was different with one of embodiment one to ten is: cutting length is that 11cm~14cm, base diameter are 0.22 cm~2.5cm in the step 3.Other is identical with one of embodiment one to ten.
Embodiment 12: what present embodiment was different with one of embodiment one to ten is: cutting length is that 13cm, base diameter are 0.25cm~1cm in the step 3.Other is identical with one of embodiment one to ten.
Embodiment 13: what present embodiment was different with one of embodiment one to 12 is: be 0.19%~0.21%(quality with concentration before the cuttage in the step 4) liquor potassic permanganate spray cutting bed, every square metre of sprinkling 2.2L~2.8L.Other is identical with one of embodiment one to 12.
Embodiment 14: what present embodiment was different with one of embodiment one to 12 is: be the 0.20%(quality with concentration before the cuttage in the step 4) liquor potassic permanganate spray cutting bed, every square metre of sprinkling 2.5L.Other is identical with one of embodiment one to 12.
Embodiment 15: what present embodiment was different with one of embodiment one to 14 is: the line space of cuttage is from being 5.3cm~5.8cm in the step 4, and distance is 3.1cm~3.4cm between the strain in every row.Other is identical with one of embodiment one to 14.
Embodiment 16: what present embodiment was different with one of embodiment one to 14 is: the line space of cuttage is from being 5.5cm in the step 4, and distance is 3.3cm between the strain in every row.Other is identical with one of embodiment one to 14.
Embodiment 17: what present embodiment was different with one of embodiment one to 16 is: in the step 4 after the cuttage with 0.095%~0.105%(quality) liquor potassic permanganate spray, every square metre is sprayed 2.2L~2.8L.Other is identical with one of embodiment one to 16.
Embodiment 18: what present embodiment was different with one of embodiment one to 16 is: use the 0.10%(quality in the step 4 after the cuttage) liquor potassic permanganate spray, every square metre is sprayed 2.5L.Other is identical with one of embodiment one to 16.
Embodiment 19: what present embodiment was different with one of embodiment one to 18 is: in the step 5 from cuttage finish to after 2.6~2.9 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85.5%~87.5%, temperature is 26.2 ℃~28.7 ℃, obscurity is 71%~74%, and the trace element fertilizer amount of application is 0.032g~0.038g/m when applying fertilizer at every turn 2, the fertile amount of application of macroelement is 6g/m 2~9g/m 2Other is identical with one of embodiment one to 18.
Embodiment 20: what present embodiment was different with one of embodiment one to 18 is: in the step 5 from cuttage finish to after 2.8 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 86%, temperature is 27 ℃, obscurity is 73%, and the trace element fertilizer amount of application is 0.035g/m when applying fertilizer at every turn 2, the fertile amount of application of macroelement is 8g/m 2Other is identical with one of embodiment one to 18.
Embodiment 21: what present embodiment was different with one of embodiment one to 20 is: remove in the step 5 on the cutting bed behind the plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85.5%~87.5%, and obscurity is 71%~74%.Other is identical with one of embodiment one to 20.
Embodiment 22: what present embodiment was different with one of embodiment one to 20 is: remove in the step 5 on the cutting bed behind the plastic film, keeping the relative moisture in the growing space of taxus chinensis in northeast seedling is 87%, and obscurity is 72%.Other is identical with one of embodiment one to 20.
Embodiment 23: present embodiment is with one of embodiment one to 22 different: grow seedlings in the greenhouse in the step 6, when the temperature in the greenhouse is low to moderate 4.2 ℃~5.8 ℃, irrigate winter protection water, keeping indoor temperature then is-12 ℃~-33 ℃, obscurity is 71%~74%, in the 3rd year spring, when temperature was stablized by 4.5 ℃ ~ 5.5 ℃, adjusting obscurity was 52%~58%.Other is identical with one of embodiment one to 22.
Embodiment 24: present embodiment is with one of embodiment one to 22 different: grow seedlings in the greenhouse in the step 6, when the temperature in the greenhouse is low to moderate 5 ℃, irrigate winter protection water, keeping indoor temperature then is-25 ℃, obscurity is 73%, in the 3rd year spring, when temperature was stablized by 5 ℃, adjusting obscurity was 55%.Other is identical with one of embodiment one to 22.
Embodiment 25: present embodiment is different with one of embodiment one to 24: grow seedlings on open country in the step 6, when the minimum air temperature is low to moderate 9.5 ℃~10.5 ℃, with plastic foil the taxus chinensis in northeast seedling is covered.Other is identical with one of embodiment one to 24.
Embodiment 26: present embodiment is different with one of embodiment one to 24: grow seedlings on open country in the step 6, when the minimum air temperature is low to moderate 10 ℃, with plastic foil the taxus chinensis in northeast seedling is covered.Other is identical with one of embodiment one to 24.
Embodiment 27: what present embodiment was different with one of embodiment one to 26 is: the mass ratio of detritus soil and river sand is 1:1.45~1.55 in the step 7.Other is identical with one of embodiment one to 26.
Embodiment 28: what present embodiment was different with one of embodiment one to 26 is: the mass ratio of detritus soil and river sand is 1:1.50 in the step 7.Other is identical with one of embodiment one to 26.
Embodiment 29: what present embodiment was different with one of embodiment one to 28 is: in the step 7 on open country the width in seedbed be that 0.95m~1.05m, thickness are 0.26m~0.29m.Other is identical with one of embodiment one to 28.
Embodiment 30: what present embodiment was different with one of embodiment one to 28 is: in the step 7 on open country the width in seedbed be that 1.0m, thickness are 0.28m.Other is identical with one of embodiment one to 28.
Embodiment 31: what present embodiment was different with one of embodiment one to 30 is: the line space of taxus chinensis in northeast seedling is from being 5.2cm~5.8cm for distance between 10.2cm~10.8cm, strain in the step 7.Other is identical with one of embodiment one to 30.
Embodiment 32: what present embodiment was different with one of embodiment one to 30 is: the line space of taxus chinensis in northeast seedling is from being 5.5cm for distance between 10.5cm, strain in the step 7.Other is identical with one of embodiment one to 30.
Embodiment 33: what present embodiment was different with one of embodiment one to 32 is: keep in the step 7 that relative moisture is 85.5%~87.5% in the growing space of Chinese yew seedling in spring nurturing period, summer and autumn, obscurity is 71%~74%.Other is identical with one of embodiment one to 32.
Embodiment 34: what present embodiment was different with one of embodiment one to 32 is: keep in the step 7 that relative moisture is 86% in the growing space of Chinese yew seedling in spring nurturing period, summer and autumn, obscurity is 73%.Other is identical with one of embodiment one to 32.
Embodiment 35: what present embodiment was different with one of embodiment one to 34 is: the amount of application of nurturing period trace element fertilizer is 0.032g~0.038g/m in the step 7 2, the amount of application of macroelement fertilizer is 6g/m 2~9g/m 2Other is identical with one of embodiment one to 34.
Embodiment 36: what present embodiment was different with one of embodiment one to 34 is: the amount of application of nurturing period trace element fertilizer is 0.035g/m in the step 7 2, the amount of application of macroelement fertilizer is 8g/m 2Other is identical with one of embodiment one to 34.
Embodiment 37: what present embodiment was different with one of embodiment one to 36 is: cover the taxus chinensis in northeast seedling with plastic foil autumn nurturing period when the minimum air temperature is low to moderate 9.5 ℃~10.5 ℃ in the step 7.Other is identical with one of embodiment one to 36.
Embodiment 38: what present embodiment was different with one of embodiment one to 36 is: cover the taxus chinensis in northeast seedling with plastic foil autumn nurturing period when the minimum air temperature is low to moderate 10 ℃ in the step 7.Other is identical with one of embodiment one to 36.
Embodiment 39: what present embodiment was different with one of embodiment one to 38 is: the HORMONE TREATMENT described in the step 3 is to be α-Nai Yisuan immersion 16h~23h of 50ppm~90ppm with skewer fringe root concentration.Other is identical with one of embodiment one to 38.
Embodiment 40: what present embodiment was different with one of embodiment one to 38 is: the HORMONE TREATMENT described in the step 3 is to be the α-Nai Yisuan immersion 20h of 70ppm with skewer fringe root concentration.Other is identical with one of embodiment one to 38.
Embodiment 41: what present embodiment was different with one of embodiment one to 38 is: the HORMONE TREATMENT described in the step 3 is to be 45 * 10 with skewer fringe root concentration -6μ L/L~65 * 10 -6Soaking with indole butyric acid 11h~13h of μ L/L.Other is identical with one of embodiment one to 38.
Embodiment 42: what present embodiment was different with one of embodiment one to 38 is: the HORMONE TREATMENT described in the step 3 is to be 50 * 10 with skewer fringe root concentration -6The soaking with indole butyric acid 12h of μ L/L.Other is identical with one of embodiment one to 38.
Embodiment 43: the method for the taxus chinensis in northeast pierre of present embodiment is carried out according to the following steps:
The method of the taxus chinensis in northeast pierre of present embodiment is carried out according to the following steps: one, select fringe: the winter in 1 year, the middle and upper part of Chinese yew tree crown was northeastward cut and is adopted 1~3 year whole lignified branch of life and make cuttage bar fringe, buries stand-by with ice and snow; Two, cutting bed is made: the spring in 1 year, be that 1:1.5 takes by weighing detritus soil and river sand and mixes by the mass ratio of detritus soil and river sand, carry out disinfection, after the desinsection processing that being made into width in the greenhouse is that 1.1m, thickness are the cutting bed of 0.25m; Three, the skewer fringe is handled: it is 15 ㎝ that the bar fringe of step 1 collection is cut into length, the plugged ear of base diameter 〉=0.2 ㎝, plugged ear have 1 terminal bud or short lateral bud, and the upper cut of plugged ear is flat mouthful, lower cut is an angle, is the α-Nai Yisuan immersion 17h of 100ppm then with concentration; Four, cuttage: with concentration is the 0.20%(quality) liquor potassic permanganate spray cutting bed, every square metre is sprayed 2L, the plugged ear that step 3 is handled is vertically inserted on the cutting bed then, the cuttage degree of depth is 1/3 of a cutting length, the line space of cuttage is from being 5cm, and distance be 3cm between the strain in every row, uses the 0.10%(quality after the cuttage immediately) the liquor potassic permanganate sprinkling, every square metre is sprayed 2L, and then washes leaf with clear water; Five, cuttage seeding management: from cuttage finish to after 2.5 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 86%, temperature is 27 ℃~28 ℃, obscurity is 72%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once sprayed fertilizer once in per ten days, and wherein the trace element fertilizer amount of application is 0.035 g/m 2, the fertile amount of application of macroelement is 8g/m 2, remove plastic film on the cutting bed then, the relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%, obscurity is 70%; Six, overwinter management: when the temperature in the greenhouse is low to moderate 5 ℃, irrigate winter protection water, keeping indoor temperature then is-10 ℃~-35 ℃, and obscurity is 75%, the three year spring, and when temperature was stablized by 5 ℃, adjusting obscurity was 60%; Seven, changing bed cultivates: the 3rd year spring, by the mass ratio of detritus soil and river sand is that 1:1.4 takes by weighing detritus soil and river sand and mixes, carry out disinfection, after desinsection is handled, being made into width on open country is 1.0m, thickness is the seedbed of 0.30m, then when temperature is stablized by 15 ℃, the taxus chinensis in northeast seedling that step 6 is cultivated is pressed line space from being 10cm, distance is implanted on the seedbed for 6cm between strain, cultivated 2 years, in biennium, spring, keep that relative moisture is 86% in the growing space of Chinese yew seedling in summer and autumn, obscurity is 75%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once, sprayed fertilizer once in per ten days, wherein the amount of application of trace element fertilizer is 0.04g/m 2, the amount of application of macroelement fertilizer is 10g/m 2With plastic foil cover taxus chinensis in northeast seedling when being low to moderate 10 ℃ when the minimum air temperature autumn, covers the straw screen or mat shading on the plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating; The taxus chinensis in northeast seedling of afforestation obtains going up a hill.
Present embodiment experimental field be located at nursery, State Forestry Administration, P.R. China green hill, reed river, Heilongjiang Province.The geographical position be 127 ° 21 of east longitude '~127 ° 25 ', 45 ° 16 '~45 ° 19 of north latitude ', 36.4 ℃ of year maximum air temperatures, the minimum air temperature-38.1 ℃, mean annual precipitation is 635.8mm, average annual relative moisture 70%.The method of present embodiment makes taxus chinensis in northeast cuttage root-taking rate reach 89.2%, planting percent reaches 85%, accelerate the speed of seedling-wood breeding, the illumination of this method, temperature, damp condition are fit to the taxus chinensis in northeast growth, make the high amount of growth of taxus chinensis in northeast year of artificial breeding reach 15cm, and about 5 cm of high amount of growth of natural taxus chinensis in northeast year~8cm, make the taxus chinensis in northeast afforestation of artificial culture get final product collection and utilization after 3 years, under the situation that does not reduce the medicinal content of taxus chinensis in northeast taxol, shortened the technical maturity of the medicinal woods of taxus chinensis in northeast; The clone taxus chinensis in northeast of artificial culture is the shrub shape more, be thick with leaves, amount of growth is big, after measured, its branches and leaves dry matter biomass yield is more than 2.5 times of natural taxus chinensis in northeast of the same age: natural living branches and leaves (dry matter) are 0.14 kilogram/strain, artificial culture be 0.3~0.5 kilogram/strain; Manually accompany the taxus chinensis in northeast of educating to cut every 1 year and gather branches and leaves once, and increase with the age of tree, its branches and leaves output increases progressively, and has guaranteed the amount of growth and the Sustainable utilization of resources of the medicinal woods maximum of Chinese yew.
Embodiment 44: the method for the taxus chinensis in northeast pierre of present embodiment was carried out according to the following steps: one, select fringe: the winter in 1 year, the middle and upper part of Chinese yew tree crown northeastward, cut and adopt 1~3 year whole lignified branch of life and make cuttage bar fringe, bury stand-by with ice and snow; Two, cutting bed is made: the spring in 1 year, be that 1:1.5 takes by weighing detritus soil and river sand and mixes by the mass ratio of detritus soil and river sand, carry out disinfection, after the desinsection processing that being made into width on open country is that 1.0m, thickness are the cutting bed of 0.30m; Three, the skewer fringe is handled: it is 15 ㎝ that the bar fringe of step 1 collection is cut into length, and base diameter is the plugged ear of 0.2cm ~ 1cm, and plugged ear has 1 terminal bud or short lateral bud, and the upper cut of plugged ear is flat mouthful, and lower cut is an angle, is 50 * 10 with concentration -6The soaking with indole butyric acid 10h of μ L/L; Four, cuttage: with concentration is the 0.20%(quality) liquor potassic permanganate spray cutting bed, every square metre is sprayed 2.5L, the plugged ear that step 3 is handled is vertically inserted on the cutting bed then, the cuttage degree of depth is 1/3 of a cutting length, the line space of cuttage is from being 6cm, and distance be 3.5cm between the strain in every row, uses the 0.10%(quality after the cuttage immediately) the liquor potassic permanganate sprinkling, every square metre is sprayed 2.5L, and then washes leaf with clear water; Five, cuttage seeding management: from cuttage finish to after 2.5 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 87%, temperature is 26 ℃~27 ℃, obscurity is 74%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once sprayed fertilizer once in per ten days, and wherein the trace element fertilizer amount of application is 0.04g/m 2, the fertile amount of application of macroelement is 8g/m 2, remove plastic film on the cutting bed then, the relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%, obscurity is 70%; Six, overwinter management: when the minimum air temperature is low to moderate 10 ℃, the taxus chinensis in northeast seedling is covered, cover the straw screen or mat shading on the plastic foil with plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating in the 3rd year spring, removes straw screen or mat when temperature is stablized by 10 ℃; Seven, changing bed cultivates: the 3rd year spring, by the mass ratio of detritus soil and river sand is that 1:1.4 takes by weighing detritus soil and river sand and mixes, carry out disinfection, after desinsection is handled, being made into width on open country is 1.0m, thickness is the seedbed of 0.30m, then when temperature is stablized by 15 ℃, the taxus chinensis in northeast seedling that step 6 is cultivated is pressed line space from being 10cm, distance is implanted on the seedbed for 6cm between strain, cultivated 2 years, in biennium, spring, keep that relative moisture is 86% in the growing space of Chinese yew seedling in summer and autumn, obscurity is 73%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once, sprayed fertilizer once in per ten days, wherein the amount of application of trace element fertilizer is 0.04g/m 2, the amount of application of macroelement fertilizer is 8 g/m 2With plastic foil cover taxus chinensis in northeast seedling when being low to moderate 10 ℃ when the minimum air temperature autumn, covers the straw screen or mat shading on the plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating; The taxus chinensis in northeast seedling of afforestation obtains going up a hill.
Present embodiment experimental field be located at nursery, State Forestry Administration, P.R. China green hill, reed river, Heilongjiang Province.The geographical position be 127 ° 21 of east longitude '~127 ° 25 ', 45 ° 16 '~45 ° 19 of north latitude ', 36.4 ℃ of year maximum air temperatures, the minimum air temperature-38.1 ℃, mean annual precipitation is 635.8mm, average annual relative moisture 70%.
The method of present embodiment makes taxus chinensis in northeast cuttage root-taking rate reach 96%, planting percent reaches 94%, accelerate the speed of seedling-wood breeding, the illumination of this method, temperature, damp condition are fit to the taxus chinensis in northeast growth, make the high amount of growth of taxus chinensis in northeast year of artificial breeding reach 18cm, and about 5 cm of high amount of growth of natural taxus chinensis in northeast year~8cm, make the taxus chinensis in northeast afforestation of artificial culture get final product collection and utilization after 3 years, under the situation that does not reduce the medicinal content of taxus chinensis in northeast taxol, shortened the technical maturity of the medicinal woods of taxus chinensis in northeast; The clone taxus chinensis in northeast of artificial culture is the shrub shape more, be thick with leaves, amount of growth is big, after measured, its branches and leaves dry matter biomass yield is more than 2.5 times of natural taxus chinensis in northeast of the same age: natural living branches and leaves (dry matter) are 0.18 kilogram/strain, artificial culture be 0.3~0.5 kilogram/strain; Manually accompany the taxus chinensis in northeast of educating to cut every 1 year and gather branches and leaves once, and increase with the age of tree, its branches and leaves output increases progressively, and has guaranteed the amount of growth and the Sustainable utilization of resources of the medicinal woods maximum of Chinese yew.

Claims (10)

1. the method for taxus chinensis in northeast pierre, the method that it is characterized in that the taxus chinensis in northeast pierre was carried out according to the following steps: one, select fringe: the winter in 1 year, the middle and upper part of Chinese yew tree crown northeastward, cut and adopt 1~3 year whole lignified branch of life and make cuttage bar fringe, bury stand-by with ice and snow; Two, cutting bed was made: the spring in 1 year, by the mass ratio of detritus soil and river sand is that 1:1.4~1.6 take by weighing detritus soil and river sand and mix, carry out disinfection, after desinsection handles, being made into width in the greenhouse or on the open country is that 0.9m~1.2m, thickness are the cutting bed of 0.25m~0.30m; Three, the skewer fringe is handled: it is 10cm~15cm that the bar fringe of step 1 collection is cut into length, and the plugged ear of base diameter 〉=0.2cm, plugged ear have 1 terminal bud or short lateral bud, use HORMONE TREATMENT then; Four, cuttage: with mass percent concentration is that 0.18%~0.22% liquor potassic permanganate sprays cutting bed, every square metre is sprayed 2L~3L, the plugged ear that step 3 is handled is vertically inserted on the cutting bed then, the cuttage degree of depth is 1/3 of a cutting length, the line space of cuttage is from being 5cm~6cm, and distance is 3cm~3.5cm between the strain in every row, is 0.09%~0.11% liquor potassic permanganate sprinkling immediately with mass percent concentration after the cuttage, every square metre is sprayed 2L~3L, and then washes leaf with clear water; Five, cuttage seeding management: from cuttage finish to after 2.5~3 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%~88%, temperature is 26 ℃~29 ℃, obscurity is 70%~75%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once sprayed fertilizer once in per ten days, and wherein the trace element fertilizer amount of application is 0.03g/m 2~0.04g/m 2, the fertile amount of application of macroelement is 5g/m 2~10g/m 2, remove plastic film on the cutting bed then, the relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85%~88%, obscurity is 70%~75%; Six, overwinter management: in the greenhouse, grow seedlings, when the temperature in the greenhouse is low to moderate 4 ℃~6 ℃, irrigate winter protection water, keeping indoor temperature then is-10 ℃~-35 ℃, and obscurity is the 70%~75%, three year spring, when temperature was stablized by 4 ℃~6 ℃, adjusting obscurity was 50%~60%; On open country, grow seedlings, when the minimum air temperature is low to moderate 11 ℃, the taxus chinensis in northeast seedling is covered, cover the straw screen or mat shading on the plastic foil with plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating in the 3rd year spring, removes straw screen or mat when temperature is stablized by 10 ℃; Seven, changing bed cultivates: the 3rd year spring, by the mass ratio of detritus soil and river sand is that 1:1.4~1.6 take by weighing detritus soil and river sand and mix, carry out disinfection, after desinsection is handled, being made into width on open country is 0.9m~1.1m, thickness is the seedbed of 0.25m~0.30m, then when temperature is stablized by 15 ℃, the taxus chinensis in northeast seedling that step 6 is cultivated is pressed line space from being 10cm~11cm, distance is implanted on the seedbed for 5cm~6cm between strain, cultivated 2 years, in biennium, spring, keep that relative moisture is 85%~88% in the growing space of Chinese yew seedling in summer and autumn, obscurity is 70%~75%, 1000 times of liquid disinfectants using carbendazim 50% wetting powder weekly once, sprayed fertilizer once in per ten days, wherein the amount of application of trace element fertilizer is 0.03g~0.04g/m 2, the amount of application of macroelement fertilizer is 5g/m 2~10g/m 2With plastic foil cover taxus chinensis in northeast seedling when being low to moderate 11 ℃ when the minimum air temperature autumn, covers the straw screen or mat shading on the plastic foil, the ventilation of film both sides, the freeze-up preceding winter protection water of irrigating; The taxus chinensis in northeast seedling of afforestation obtains going up a hill; Wherein the HORMONE TREATMENT described in the step 3 is meant with skewer fringe root concentration to be that the α-Nai Yisuan of 40ppm~100ppm is soaked 15h~24h or is 40 * 10 with skewer fringe root concentration -6μ L/L~70 * 10 -6Soaking with indole butyric acid 10h~14h of μ L/L.
2. the method for taxus chinensis in northeast pierre according to claim 1 is characterized in that the river sand diameter 1mm~0.1mm described in the step 2.
3. the method for taxus chinensis in northeast pierre according to claim 1 and 2, it is characterized in that the sterilization described in step 2 and the step 7, desinsection are handled carries out according to the following steps: 500 times of liquid with carbendazim 50% wetting powder spray soil, and every square metre is 2L~3L with the amount of spraying.
4. the method for taxus chinensis in northeast pierre according to claim 1 and 2 is characterized in that the mass ratio of detritus soil and river sand is 1:1.45~1.55 in the step 2.
5. the method for taxus chinensis in northeast pierre according to claim 1 and 2, the width that it is characterized in that cutting bed in the step 2 are that 0.95m~1.15m, thickness are 0.26m~0.28m.
6. the method for taxus chinensis in northeast pierre according to claim 1 and 2 is characterized in that cutting length is that 11cm~14cm, base diameter are 0.22 cm~2.5cm in the step 3.
7. the method for taxus chinensis in northeast pierre according to claim 1 and 2 is characterized in that before the cuttage in the step 4 with mass percent concentration being that 0.19%~0.21% liquor potassic permanganate sprays cutting bed, every square metre of sprinkling 2.2L~2.8L.
8. the method for taxus chinensis in northeast pierre according to claim 1 and 2, the line space that it is characterized in that cuttage in the step 4 is from being 5.3cm~5.8cm, distance is 3.1cm~3.4cm between the strain in every row.
9. the method for taxus chinensis in northeast pierre according to claim 1 and 2, it is characterized in that in the step 5 from cuttage finish to after 2.6~2.9 months time in, on cutting bed, cover plastic film, relative moisture in the growing space of maintenance taxus chinensis in northeast seedling is 85.5%~87.5%, temperature is 26.2 ℃~28.7 ℃, obscurity is 71%~74%, and the trace element fertilizer amount of application is 0.032g~0.038g/m when applying fertilizer at every turn 2, the fertile amount of application of macroelement is 6g/m 2~9g/m 2
10. the method for taxus chinensis in northeast pierre according to claim 1 and 2, the line space that it is characterized in that taxus chinensis in northeast seedling in step 7 distance between for 10.2cm~10.8cm, strain is 5.2cm~5.8cm.
CN201010298148XA 2010-09-30 2010-09-30 Method for asexually propagating taxus cuspidata CN101982050B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201010298148XA CN101982050B (en) 2010-09-30 2010-09-30 Method for asexually propagating taxus cuspidata

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201010298148XA CN101982050B (en) 2010-09-30 2010-09-30 Method for asexually propagating taxus cuspidata

Publications (2)

Publication Number Publication Date
CN101982050A CN101982050A (en) 2011-03-02
CN101982050B true CN101982050B (en) 2011-09-21

Family

ID=43618982

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201010298148XA CN101982050B (en) 2010-09-30 2010-09-30 Method for asexually propagating taxus cuspidata

Country Status (1)

Country Link
CN (1) CN101982050B (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102792831B (en) * 2011-05-26 2015-07-15 汉中隆康生物科技开发有限公司 High-efficiency rapid propagation technique for Chinese yew
CN102282995B (en) * 2011-06-27 2012-10-31 江苏省中国科学院植物研究所 Cold-resistant excellent lance-leaved red anise seedling cutting propagation technology
CN102577822B (en) * 2012-03-17 2013-12-25 常熟市尚湖农业生态园有限公司 Cuttage propagation method for bald cypress
CN103109666A (en) * 2013-02-25 2013-05-22 江西省喜果绿化有限公司 Chinese yew dry-bed cutting seedling method
CN103109665A (en) * 2013-02-25 2013-05-22 江西省喜果绿化有限公司 Cutting propagation method for Chinese yew by seedling pots
CN104429430A (en) * 2013-09-17 2015-03-25 贵州省印江自治县梵净青茶业有限责任公司 Method for planting taxuschinensis tea in garden
CN103975737A (en) * 2014-05-16 2014-08-13 四川农业大学 Chinese yew vegetative propagation method
CN104429480A (en) * 2014-11-07 2015-03-25 江苏省中国科学院植物研究所 Taxodium zhongshansha 406 hard wood cutting simple culture method
CN104782457A (en) * 2015-04-03 2015-07-22 中国科学院东北地理与农业生态研究所 Fertilization method of taxus cuspidata container seedlings
CN105917936B (en) * 2016-05-16 2018-12-04 浙江农林大学 A kind of China fir Light media cuttage vessel seeding cultivating method
CN106386061A (en) * 2016-08-30 2017-02-15 崀山珍稀植物研究所 Breeding method for cathaya argyrophylla
CN107439299A (en) * 2017-08-02 2017-12-08 北京林业大学 A kind of method that taxus chinensis in northeast is cultivated in Introduced in Beijing Area
CN108040719A (en) * 2017-12-13 2018-05-18 佛山市高明区生产力促进中心 A kind of planting method of taxodium ascendens
CN109005970A (en) * 2018-07-27 2018-12-18 铜仁学院 A kind of method of summer cuttage Chinese yew

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1615682A (en) * 2004-10-21 2005-05-18 李万祥 Method for planting Chinese yew
CN1759653A (en) * 2005-10-28 2006-04-19 林敏� Method for planting southerm yew
CN101663946A (en) * 2009-09-28 2010-03-10 苏庆良 Cuttage breeding method of taxus chinensis in northeast

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1615682A (en) * 2004-10-21 2005-05-18 李万祥 Method for planting Chinese yew
CN1759653A (en) * 2005-10-28 2006-04-19 林敏� Method for planting southerm yew
CN101663946A (en) * 2009-09-28 2010-03-10 苏庆良 Cuttage breeding method of taxus chinensis in northeast

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
滕国旭等.东北红豆杉营造林技术.《吉林林业科技》.2002,第31卷(第5期),第45、47页. *

Also Published As

Publication number Publication date
CN101982050A (en) 2011-03-02

Similar Documents

Publication Publication Date Title
CN103814745B (en) Method for cutting propagation by utilizing lithocarpus polystachyus epicormic branches
CN105993626B (en) A kind of breeding method of potting Camellia nitidissima
CN105103856B (en) A kind of is suitable for the method for cultivating Moringa oleifera in northern area
CN103918530B (en) A kind of cowpea high-yield implantation methods
CN102812838B (en) Propagation method of camellia amplexicaulis cohen stuart by using shoot cutting
CN102986445B (en) Process for cutting thick branches of golden camellia
CN104106364B (en) The propagation method of a kind of mao of purple camellia sprout cuttage
CN104380994B (en) The mass-producing high yield cultivating method of a kind of bletilla striata
CN102138408B (en) Tea light-medium plug seedling method
CN101036446B (en) Quick propagating method of megranate
CN102246645B (en) High-yield culture technology for southern potatoes planted in autumn
CN102763551B (en) Cuttage and breeding technology for ampelopsis grossedentata
CN102612971B (en) Rose cutting propagation method
CN103733849B (en) A kind of golden camellia old branch cuttage propagation method
CN104585007B (en) The cuttage and seedling culture method of American red-maple
CN104920146A (en) Propagation method for rapid spikelet breeding of ithocarpus polysachyus rehd
CN104221655B (en) The method of a kind of children orchard in age interplanting leaf vegetables type sweet potato
CN102907244B (en) Short-scion cutting propagation method for star anise
CN104106382B (en) The propagation method of a kind of mao of purple camellia grafting Ta Mu island Camellia nitidissima
CN105191763A (en) Vegetable organic soil-less culture method, culture medium and manufacturing method thereof
CN100356838C (en) Season-opposite culture method of peach
CN105409677A (en) Planting method of blueberries
CN102204489B (en) Method for culturing teak seedlings by spike culture and rapid propagation in container
CN105210635A (en) A kind of honeysuckle cuttage and seedling culture method
CN102960172A (en) Short-spike and root-grafting rapid propagation method for camellia azalea

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant