CN108935969A - A kind of soluble dietary fiber, agent of feed for nursing sow and its preparation method and application - Google Patents
A kind of soluble dietary fiber, agent of feed for nursing sow and its preparation method and application Download PDFInfo
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- CN108935969A CN108935969A CN201810559171.6A CN201810559171A CN108935969A CN 108935969 A CN108935969 A CN 108935969A CN 201810559171 A CN201810559171 A CN 201810559171A CN 108935969 A CN108935969 A CN 108935969A
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 44
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 30
- 230000000474 nursing effect Effects 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 108
- 239000007788 liquid Substances 0.000 claims abstract description 45
- 238000000855 fermentation Methods 0.000 claims abstract description 42
- 230000004151 fermentation Effects 0.000 claims abstract description 33
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 32
- 239000000706 filtrate Substances 0.000 claims abstract description 23
- 239000000203 mixture Substances 0.000 claims abstract description 22
- 241000194017 Streptococcus Species 0.000 claims abstract description 19
- 108010059892 Cellulase Proteins 0.000 claims abstract description 14
- 229940106157 cellulase Drugs 0.000 claims abstract description 14
- 230000000050 nutritive effect Effects 0.000 claims abstract description 14
- 108091005804 Peptidases Proteins 0.000 claims abstract description 13
- 239000004365 Protease Substances 0.000 claims abstract description 13
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 13
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 56
- 241000894006 Bacteria Species 0.000 claims description 37
- 239000004310 lactic acid Substances 0.000 claims description 28
- 235000014655 lactic acid Nutrition 0.000 claims description 28
- 244000017020 Ipomoea batatas Species 0.000 claims description 20
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 15
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 12
- 235000005822 corn Nutrition 0.000 claims description 12
- 235000009566 rice Nutrition 0.000 claims description 12
- 150000003839 salts Chemical class 0.000 claims description 12
- 235000015099 wheat brans Nutrition 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 11
- 239000004519 grease Substances 0.000 claims description 10
- 244000068988 Glycine max Species 0.000 claims description 9
- 235000010469 Glycine max Nutrition 0.000 claims description 9
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 9
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 230000006651 lactation Effects 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 230000008020 evaporation Effects 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims 2
- 238000011081 inoculation Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 230000000968 intestinal effect Effects 0.000 abstract description 2
- 235000019629 palatability Nutrition 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 description 17
- 239000000835 fiber Substances 0.000 description 12
- 241000209094 Oryza Species 0.000 description 10
- 206010010774 Constipation Diseases 0.000 description 8
- 239000012141 concentrate Substances 0.000 description 6
- 235000021050 feed intake Nutrition 0.000 description 6
- 244000005700 microbiome Species 0.000 description 5
- 239000001913 cellulose Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 239000013065 commercial product Substances 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 230000035606 childbirth Effects 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 238000010564 aerobic fermentation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008855 peristalsis Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 235000002918 Fraxinus excelsior Nutrition 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000002956 ash Substances 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
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- Birds (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
The present invention provides a kind of soluble dietary fibers, agent of feed for nursing sow and its preparation method and application, belong to pannage technical field.The soluble dietary fiber includes the following steps to be prepared: 1) preparing streptococcus acidi lactici fermented solution；2) citric acid mixture is prepared；3) obtained citric acid mixture is used into fermentation of Aspergillus niger, obtains fermentation of Aspergillus niger liquid；4) fermentation of Aspergillus niger liquid is mixed with cellulase, pectase and protease, prepares enzymolysis liquid；5) enzymolysis liquid is filtered, collects filtrate and filter residue respectively, obtained filtrate is soluble dietary fiber.The soluble dietary fiber that the present invention is prepared can improve function of intestinal canal, and nutritive value is high, easily absorb.Further, the present invention also provides a kind of agent of feed for nursing sow including soluble dietary fiber described in above scheme, have the characteristics that nutrition is high, easy to digest and palatability, can be improved the production performance of sow.
Description
Technical field
The invention belongs to pannage art fields, and in particular to a kind of soluble dietary fiber, agent of feed for nursing sow and its system
Preparation Method and application.
Background technique
China is the first in the world pig raising big country, and 2017, the Animal Husbandry in China output value delivered 6.89 for sale up to 3.6 trillion yuans, live pig
Hundred million, wherein sow livestock on hand forty-two million head.In pig production, it is production management that there is a phenomenon where constipation is relatively common for sow
In a vast pig production person of puzzlement obstinate problem, about 40%~60% sow has constipation phenomenon according to statistics.For a long time
The sow of constipation will lead to birth process and extend even difficult labour, and the raising of serious person's body temperature is disease accompanied, stillborn foetus occur, induces agalasisa
Syndrome.
Fiber is known as except this six big nutrient of protein, fat, carbohydrate, vitamin, minerals and water
The seventh-largest nutrient, appropriate intake can help gastrointestinal peristalsis, promote the digestion and absorption of food, anti-to treat constipation, while can be
Numerous harmful, toxic factors are taken out of in vitro.One of the main reason for prevention of sow constipation be exactly in daily ration content of cellulose it is insufficient or
Cellulose is poor quality.If crude fiber content is insufficient in farrowing sow daily ration or cellulose is poor quality, enteron aisle cannot be drawn
Stimulation enough is played, intestines peristalsis is made to slow down and cause constipation.The quality of agent of feed for nursing sow quality and cellulose nutrition are not only straight
Lactation amount, the survival rate of weanling pig and the weanling weight of piglet for influencing sow are connect, but also will affect sow after wean
Normal heat, breeding.Therefore, it is extremely important to improve milking sow feed intake.However, the conventional generally existing fiber of fiber source
The problems such as content is not high, and mycotoxin contamination is serious, and fermentable fibres ratio is low, and content of mineral substances is uneven, to influence fibre
The application in sow diets preparation is tieed up, the development of live pig industry is constrained.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of soluble dietary fiber, agent of feed for nursing sow and its preparations
Methods and applications can prevent and treat prevention of sow constipation, improve feed intake, promote milking sow lactation.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of soluble dietary fibers, are prepared by the method included the following steps:
1) sweet potato vine powder, glucose, nutritive salt, water and lactic acid bacteria are blended in anaerobic fermentation 18 at 25~30 DEG C~for 24 hours,
Obtain streptococcus acidi lactici fermented solution；
2) streptococcus acidi lactici fermented solution of the step 1) is mixed with citric acid, obtains citric acid mixture；
The mass ratio of the sweet potato vine powder and citric acid is 100:1~5；
3) citric acid mixture of the step 2) is adjusted into pH value to inoculated aspergillus niger after 6~7, is issued at 28~32 DEG C
36~72h of ferment obtains fermentation of Aspergillus niger liquid；
4) the fermentation of Aspergillus niger liquid of the step 3) is mixed with cellulase, pectase and protease, it is mixed by what is obtained
Synthase liquid carries out 8~12h of enzymatic hydrolysis at 35~50 DEG C, obtains enzymolysis liquid；
5) enzymolysis liquid of the step 4) is filtered, collects filtrate and filter residue respectively, obtained filtrate is soluble dietary
Fiber.
Preferably, the sweet potato vine powder of the step 1), glucose, nutritive salt, water and the mass ratio of lactic acid bacteria be 100:3~
5:0.2~0.5:200~400:5~10.
Preferably, the viable bacteria concentration of lactic acid bacteria is 1.0 × 10 in the step 1)6~1.0 × 109CFU/g。
Preferably, the solution for pH value being adjusted in the step 3) is calcium carbonate soln, potassium hydroxide solution or hydroxide
Sodium solution.
Preferably, the mass ratio of citric acid mixture aspergillus niger bacterium solution is 100:5~10 in the step 3).
Preferably, the mass ratio of fermentation of Aspergillus niger liquid in the step 4), cellulase, pectase and protease is 100:
0.1~0.5:0.01~0.05:0.005~0.05.
Preferably, after the step 4) obtains filtrate further include: the filtrate is concentrated；The method of the concentration is
It is concentrated by evaporation under conditions of 50~60 DEG C.
The present invention provides a kind of agent of feed for nursing sow, the component including following parts by weight: solvable described in above scheme
130~140 parts of dietary fiber of property, 60~70 parts of filter residue, 160~170 parts of corn, crack rice 6~16 parts, it is 55~65 parts of dregs of beans, swollen
15~20 parts of soybean of change, 15~20 parts of wheat bran, 15~20 parts of grease and 17~19 parts of premix；
The filter residue is the filter residue that soluble dietary fiber generation is prepared in above scheme.
The present invention provides a kind of preparation methods of agent of feed for nursing sow described in above scheme, by the soluble dietary
Fiber filter residue, corn, is cracked rice, dregs of beans, expanded soybean, wheat bran, grease and premix mixing, dry at 50~60 DEG C, is obtained
Agent of feed for nursing sow.
The present invention provides agent of feed for nursing sow described in a kind of above scheme or the method as described in above scheme to prepare
Application of the obtained agent of feed for nursing sow in feeding milking sow.
The present invention provides a kind of soluble dietary fibers, are prepared by the method included the following steps: 1) preparation cream
Acid bacteria fermentation liquid；2) citric acid mixture is prepared；3) obtained citric acid mixture inoculated aspergillus niger is fermented, obtains aspergillus niger
Fermentation liquid；4) fermentation of Aspergillus niger liquid is mixed with cellulase, pectase and protease, prepares enzymolysis liquid；5) by enzymolysis liquid mistake
Filter, collects filtrate and filter residue respectively, and obtained filtrate is soluble dietary fiber.When pH is lower than 3~4, many microorganisms are (outstanding
It is putrefactive microorganisms) growth suffers from inhibitions, or even death.In anaerobic fermentation process, the lactic acid that lactic acid bacteria generates can
Fermentation liquid pH is reduced to 4 hereinafter, the growth of other microorganisms at this time is suppressed, but works as the lactic acid accumulation in fermentation liquid
When to a certain concentration, but can lactic acid bacteria inhibiting itself proliferation.After lactic acid bacteria anaerobic fermentation is first generated a large amount of lactic acid by the present invention,
Fermentation liquid pH can be reduced to 3 hereinafter, can be (especially corrupt by miscellaneous bacteria inactivation in fermentation liquid by adding a certain amount of citric acid
Microorganism), be conducive to subsequent aspergillus niger aerobic fermentation, and be not spoilage organisms occur largely to be proliferated, while aspergillus niger is being sent out
Amylase, cellulase, pectase etc. are generated during ferment, is digested in fermentation, add cellulase, pectase and
Protease hydrolyzed prepares soluble dietary fiber.And the soluble dietary fiber that is prepared of the present invention be suitable for lactic acid bacteria and
The fermentation of butyric, promotes the absorption of nutriment, while inhibiting the growth of harmful bacteria in enteron aisle, improves function of intestinal canal.Simultaneously
Soluble dietary fiber provided by the invention, content of ashes is low, does not influence animal electrolyte balance.
Further, the present invention also provides a kind of, and the milking sow including soluble dietary fiber described in above scheme is raised
Material, by soluble dietary fiber, insoluble diedairy fiber, corn, crack rice, dregs of beans, expanded soybean, wheat bran and premix are by certain
Ratio is prepared, and the clover fiber feedstuff that the present invention is prepared is rich in soluble dietary fiber, reduced sugar, amino acid and biology
Enzyme has the characteristics that nutrition is high, easy to digest and palatability, can effectively improve feed intake, reduces constipation rate and promotes lactation
Sow lactation.The effect of embodiment shows after feeding agent of feed for nursing sow provided by the invention that the feed intake of milking sow is compared
It is respectively increased 30.78%~32.82% in control group, piglet daily gain improves 22.63%~25.61% than control group respectively,
The piglet death rate reduces by 77.11%~79.42% than control group respectively.
Specific embodiment
The present invention provides a kind of soluble dietary fibers, are prepared by the method included the following steps:
1) sweet potato vine powder, glucose, nutritive salt, water and lactic acid bacteria are blended in anaerobic fermentation 18 at 25~30 DEG C~for 24 hours,
Obtain streptococcus acidi lactici fermented solution；
2) streptococcus acidi lactici fermented solution of the step 1) is mixed with citric acid, obtains citric acid mixture；
The mass ratio of the sweet potato vine powder and citric acid is 100:1~5；
3) citric acid mixture of the step 2) is adjusted into pH value to inoculated aspergillus niger after 6~7, is issued at 28~32 DEG C
36~72h of ferment obtains fermentation of Aspergillus niger liquid；
4) the fermentation of Aspergillus niger liquid of the step 3) is mixed with cellulase, pectase and protease, it is mixed by what is obtained
Synthase liquid carries out 8~12h of enzymatic hydrolysis at 35~50 DEG C, obtains enzymolysis liquid；
5) enzymolysis liquid of the step 4) is filtered, collects filtrate and filter residue respectively, obtained filtrate is soluble dietary
Fiber.
The present invention by sweet potato vine powder, glucose, nutritive salt, water and lactic acid bacteria be blended in anaerobic fermentation 18 at 25~30 DEG C~
For 24 hours, streptococcus acidi lactici fermented solution is obtained；.In the present invention, the granularity of the sweet potato vine powder is preferably 100~200 mesh, more preferably 150
Mesh.The sweet potato vine powder, glucose, nutritive salt, water and the mass ratio of lactic acid bacteria be preferably 100:3~5:0.2~0.5:200~
400:5~10, more preferably 100:4:0.35:300:8.The viable bacteria concentration of the lactic acid bacteria is preferably 1.0 × 106~1.0 ×
109CFU/g, more preferably 1.0 × 108CFU/g.The mixed mode preferably stirs.The revolving speed of the stirring is preferably
1000~2000r/min, more preferably 1500r/min.The time of the stirring is preferably 3~10min, more preferably 5min.
Sweet potato vine powder, glucose, nutritive salt, water and lactic acid bacteria are mixed and carry out anaerobic fermentation by the present invention, are generated a large amount of lactic acid, are conducive to
Enteron aisle is digested and assimilated.
The present invention is not particularly limited the sweet potato vine powder, glucose, nutritive salt, water and the source of lactic acid bacteria, uses
This field conventional commercial product.After obtaining streptococcus acidi lactici fermented solution, the present invention is by the streptococcus acidi lactici fermented solution and lemon acid-mixed
It closes, obtains citric acid mixture；The mass ratio of the sweet potato vine powder and citric acid is 100:1~5.In the present invention, the mixing
Mode preferably stir.The revolving speed of the stirring is preferably 1000~2000r/min, more preferably 1500r/min.It is described to stir
The time mixed is preferably 3~10min, more preferably 5min.The mass ratio of the streptococcus acidi lactici fermented solution and citric acid is preferably
100:3.In the present invention, streptococcus acidi lactici fermented solution is mixed with citric acid, reduces the pH value of streptococcus acidi lactici fermented solution, make microorganism
Growth is suppressed, while preventing the reproduction of the excessive fermentation lactic acid bacteria itself always of lactic acid bacteria.
The present invention is not particularly limited the source of the citric acid, using this field conventional commercial product.
After obtaining citric acid mixture, the citric acid mixture is adjusted pH value to inoculated aspergillus niger after 6~7 by the present invention
Bacterium solution, 36~72h of aerobic fermentation at 28~32 DEG C, obtains fermentation of Aspergillus niger liquid.In the present invention, the adjusting pH value solution
For calcium carbonate soln, potassium hydroxide solution or sodium hydroxide solution.The mass ratio of the citric acid mixture aspergillus niger bacterium solution is
100:5~10, more preferably 100:8.The mixed mode preferably stirs.The revolving speed of the stirring is preferably 1000~
2000r/min, more preferably 1500r/min.The time of the stirring is preferably 3~10min, more preferably 5min.The hair
The temperature of ferment is preferably 30 DEG C.The time of the fermentation is preferably 48~60h, more preferably 55h.
The present invention is not particularly limited the source of the aspergillus niger, using this field conventional commercial product.
After obtaining fermentation of Aspergillus niger liquid, the present invention is by the fermentation of Aspergillus niger liquid and cellulase, pectase and protease
Mixing, carries out 8~12h of enzymatic hydrolysis for obtained mixed enzyme solution at 35~50 DEG C, obtains enzymolysis liquid.In the present invention, the black song
The mass ratio of mould fermentation liquid and cellulase, pectase and protease is preferably 100:0.1~0.5:0.01~0.05:0.005
~0.05, more preferably 100:0.3:0.03:0.03.The temperature of the enzymatic hydrolysis is preferably 40~45 DEG C, and more preferably 42 DEG C, institute
The time for stating enzymatic hydrolysis is preferably 10h.
After obtaining enzymolysis liquid, the present invention filters the enzymolysis liquid, collects filtrate and filter residue respectively, obtained filtrate is can
Soluble dietary fiber.
In the present invention, the aperture of filter membrane used by the filtering is preferably 50~100 mesh, more preferably 80 mesh.This hair
In bright, preferably gained filtrate is preferably concentrated after filtering, obtained concentrate is soluble dietary fiber.The concentration
Method is preferably concentrated by evaporation under conditions of 50~60 DEG C, and more preferably 55 DEG C.In the present invention, the concentrate that is obtained after concentration
The percentage composition that middle soluble dietary fiber accounts for concentrate gross mass is preferably 60%~70%, and more preferably 65%.
The present invention provides a kind of agent of feed for nursing sow, the component including following parts by weight: solvable described in above scheme
130~140 parts of dietary fiber of property, 60~70 parts of filter residue, 160~170 parts of corn, crack rice 6~16 parts, it is 55~65 parts of dregs of beans, swollen
15~20 parts of soybean of change, 15~20 parts of wheat bran, 15~20 parts of grease and 17~19 parts of premix；The filter residue is above scheme institute
It states and prepares filter residue obtained in soluble dietary fiber.
In the present invention, the agent of feed for nursing sow preferably includes the component of following parts by weight: solvable described in above scheme
65 parts of filter residue, 165 parts of corn, crack rice 10 parts, 60 parts of dregs of beans, expanded soybean 18 described in 135 parts of dietary fiber of property, above scheme
Part, 18 parts of wheat bran and 18 parts of premix.
The present invention to the corn, crack rice, the source of dregs of beans, expanded soybean, wheat bran, grease and premix does not have special limit
It is fixed, using this field conventional commercial product.
The present invention provides a kind of preparation methods of agent of feed for nursing sow described in above scheme, by the soluble dietary
Fiber filter residue, corn, is cracked rice, dregs of beans, expanded soybean, wheat bran, grease and premix mixing, dry at 50~60 DEG C, is obtained
Agent of feed for nursing sow.
In the present invention, the mixed mode is preferably stirred.The time of the stirring is preferably 10~20min, more excellent
It is selected as 15min.The revolving speed of the stirring is preferably 2000~5000r/min, more preferably 3500r/min.The temperature of the drying
Preferably 55 DEG C of degree.
The present invention provides agent of feed for nursing sow described in a kind of above scheme or the method as described in above scheme to prepare
Application of the obtained agent of feed for nursing sow in feeding milking sow.
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
Weigh sweet potato vine powder 100Kg, glucose 3Kg, nutritive salt 0.5Kg, water 200Kg and the lactic acid bacteria (viable bacteria of lactic acid bacteria
Concentration is 1.0 × 106CFU/g) 10Kg stirs mixture after 3min the anaerobic fermentation at 25 DEG C with the revolving speed of 2000r/min
For 24 hours, streptococcus acidi lactici fermented solution is obtained.Obtained streptococcus acidi lactici fermented solution is mixed into (the quality of sweet potato vine powder and citric acid with citric acid
PH value is adjusted to being mixed after 7 with aspergillus niger than for 20:1), obtaining citric acid mixture, and ferment 72h at 28 DEG C, obtains black song
Mould fermentation liquid.By obtained fermentation of Aspergillus niger liquid and cellulase, pectase and protease by 100:0.1:0.05:0.005's
Mass ratio mixing, carries out enzymatic hydrolysis 12h at 35 DEG C, filters, and collects filtrate and filter residue respectively, and obtained filtrate is soluble dietary
Fiber.
It is big to weigh Soluble Fiber concentrate 130Kg, filter residue 70Kg, corn 160Kg, the 16Kg that cracks rice, dregs of beans 55Kg, extruding
Beans 20Kg, wheat bran 15Kg, grease 15Kg and premix 17Kg are done at 50 DEG C after stirring 20min with the revolving speed of 2000r/min
It is dry.Obtain agent of feed for nursing sow.
Embodiment 2
Weigh sweet potato vine powder 100Kg, glucose 5Kg, nutritive salt 0.2Kg, water 400Kg and the lactic acid bacteria (viable bacteria of lactic acid bacteria
Concentration is 1.0 × 109CFU/g) 5Kg stirs mixture after 3min the anaerobic fermentation at 30 DEG C with the revolving speed of 2000r/min
18h obtains streptococcus acidi lactici fermented solution.Obtained streptococcus acidi lactici fermented solution is mixed into (the quality of sweet potato vine powder and citric acid with citric acid
PH value is adjusted to being mixed after 6 with aspergillus niger than for 100:1), obtaining citric acid mixture, and ferment 36h at 32 DEG C, obtains black song
Mould fermentation liquid.By obtained fermentation of Aspergillus niger liquid with cellulase, pectase and protease by the matter of 100:0.5:0.01:0.05
Amount carries out enzymatic hydrolysis 8h than mixing at 50 DEG C, filters, and collects filtrate and filter residue respectively, and obtained filtrate is that soluble dietary is fine
Dimension.
It is big to weigh Soluble Fiber concentrate 140Kg, filter residue 60Kg, corn 170Kg, the 6Kg that cracks rice, dregs of beans 65Kg, extruding
Beans 15Kg, wheat bran 20Kg, grease 20Kg and premix 19Kg are done at 60 DEG C after stirring 10min with the revolving speed of 5000r/min
It is dry.Obtain agent of feed for nursing sow.
Embodiment 3
Weigh sweet potato vine powder 100Kg, glucose 4Kg, nutritive salt 0.35Kg, water 300Kg and the lactic acid bacteria (viable bacteria of lactic acid bacteria
Concentration is 1.0 × 108CFU/g) 8Kg stirs mixture after 3min the anaerobic fermentation at 28 DEG C with the revolving speed of 2000r/min
20h obtains streptococcus acidi lactici fermented solution.Obtained streptococcus acidi lactici fermented solution is mixed into (the quality of sweet potato vine powder and citric acid with citric acid
PH value is adjusted to being mixed after 6.5 with aspergillus niger than for 100:3), obtaining citric acid mixture, and ferment 55h at 30 DEG C, obtains black
Aspergillus fermentation broth.By obtained fermentation of Aspergillus niger liquid and cellulase, pectase and protease by 100:0.3:0.03:0.03's
Mass ratio mixing, carries out enzymatic hydrolysis 10h at 42 DEG C, filters, and collects filtrate and filter residue respectively, and obtained filtrate is soluble dietary
Fiber.
It is big to weigh Soluble Fiber concentrate 135Kg, filter residue 65Kg, corn 165Kg, the 10Kg that cracks rice, dregs of beans 60Kg, extruding
Beans 18Kg, wheat bran 18Kg, grease 18Kg and premix 18Kg are done at 55 DEG C after stirring 15min with the revolving speed of 3500r/min
It is dry.Obtain agent of feed for nursing sow.
Embodiment 4
The content of main active substances, concrete outcome in the agent of feed for nursing sow that measurement Examples 1 to 3 is prepared respectively
As shown in table 1.
The content of main active substances in the different agent of feed for nursing sow of table 1
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Color form | Yellowish-brown | It is light yellow | It is light yellow |
| Moisture/% | ≤12.0 | ≤12.0 | ≤12.0 |
| Crude protein/% | ≥15.0 | ≥15.0 | ≥15.0 |
| Crude fat/% | ≥1.5 | ≥1.5 | ≥1.5 |
| Crude fibre/% | ≤10.0 | ≤10.0 | ≤10.0 |
| Total calcium/% | ≥0.8 | ≥0.8 | ≥0.8 |
| Total phosphorus/% | ≥0.5 | ≥0.5 | ≥0.5 |
| Lysine/% | ≥0.6 | ≥0.6 | ≥0.6 |
| Methionine/% | ≥0.3 | ≥0.3 | ≥0.3 |
As can be seen from Table 1, the moisture content for the agent of feed for nursing sow that the present invention is prepared % below 12%, thick egg
It is white that 15% or more, crude fibre is less than 10%, and total calcium is 0.8% or more, and for total phosphorus 0.5% or more, lysine is greater than 0.6%,
Methionine is greater than 0.3%, nutritive value with higher.
Embodiment 5
Health Duroc milking sow 120 similar in selection body condition, parity, weight and the expected date of childbirth, is randomly divided into 4 groups,
Every group of 5 repetitions, 1 repetition, 6 sows, as a control group and experimental group.Experimental group 1~3 feeds the embodiment of the present application 1 respectively
The feed of~3 preparations.Since feed feeding experiment terminate when feeding experiment material to piglet wean in 22 days giving a birth first 5 days.Childbirth
Preceding is pre-feeding period, is positive after childbirth the examination phase.Control group is consistent with the diet nutrient of experimental group, the battalion such as protein, energy and calcium, phosphorus
Index difference is supported less than 0.005%.Measure feed intake, daily gain and the piglet death rate of sow.Concrete outcome is as shown in table 2.
Influence of 2. different feeds of table to milking sow production performance
As can be seen from Table 2, after feeding agent of feed for nursing sow provided by the invention, the feed intake of milking sow is compared to right
It is respectively increased 30.78%~32.82% according to group, piglet daily gain improves 22.63%~25.61% than control group respectively, piglet
The death rate reduces by 77.11%~79.42% than control group respectively.The result shows that: the agent of feed for nursing sow that the present invention is prepared
The production performance of milking sow can be significantly improved.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of soluble dietary fiber, which is characterized in that be prepared by the method included the following steps:
1) sweet potato vine powder, glucose, nutritive salt, water and lactic acid bacteria are blended in anaerobic fermentation 18 at 25~30 DEG C~for 24 hours, obtained
Streptococcus acidi lactici fermented solution；
2) streptococcus acidi lactici fermented solution of the step 1) is mixed with citric acid, obtains citric acid mixture；
The mass ratio of the sweet potato vine powder and citric acid is 100:1~5；
3) citric acid mixture of the step 2) is adjusted into pH value to inoculation black-koji mould liquid after 6~7, is issued at 28~32 DEG C
36~72h of ferment obtains fermentation of Aspergillus niger liquid；
4) the fermentation of Aspergillus niger liquid of the step 3) is mixed with cellulase, pectase and protease, the mixed enzyme that will be obtained
Liquid carries out 8~12h of enzymatic hydrolysis at 35~50 DEG C, obtains enzymolysis liquid；
5) enzymolysis liquid of the step 4) is filtered, collects filtrate and filter residue respectively, obtained filtrate is soluble dietary fiber.
2. soluble dietary fiber according to claim 1, which is characterized in that the sweet potato vine powder of the step 1), grape
Sugar, nutritive salt, water and the mass ratio of lactic acid bacteria are 100:3~5:0.2~0.5:200~400:5~10.
3. soluble dietary fiber according to claim 1 or 2, which is characterized in that the work of lactic acid bacteria in the step 1)
Bacteria concentration is 1.0 × 106~1.0 × 109CFU/g。
4. soluble dietary fiber according to claim 1, which is characterized in that adjust the molten of pH value in the step 3)
Liquid is calcium carbonate soln, potassium hydroxide solution or sodium hydroxide solution.
5. soluble dietary fiber according to claim 1 or 4, which is characterized in that citric acid mixes in the step 3)
The mass ratio of liquid aspergillus niger bacterium solution is 100:5~10.
6. soluble dietary fiber according to claim 1, which is characterized in that fermentation of Aspergillus niger liquid in the step 4),
The mass ratio of cellulase, pectase and protease is 100:0.1~0.5:0.01~0.05:0.005~0.05.
7. soluble dietary fiber according to claim 1, which is characterized in that the step 4) is also wrapped after obtaining filtrate
It includes: the filtrate is concentrated；The method of the concentration is to be concentrated by evaporation under conditions of 50~60 DEG C.
8. a kind of agent of feed for nursing sow, which is characterized in that the component including following parts by weight: claim 1~7 any one institute
130~140 parts of the soluble dietary fiber stated, 60~70 parts of filter residue, 160~170 parts of corn, crack rice 6~16 parts, dregs of beans 55~
65 parts, 15~20 parts of expanded soybean, 15~20 parts of wheat bran, 15~20 parts of grease and 17~19 parts of premix；
The filter residue is the filter residue that claim 1~7 any one prepares soluble dietary fiber generation.
9. the preparation method of agent of feed for nursing sow according to any one of claims 8, which is characterized in that by the soluble dietary fiber,
Filter residue corn, is cracked rice, dregs of beans, expanded soybean, wheat bran, grease and premix mixing, dry at 50~60 DEG C, obtains lactation
Sow feed.
10. agent of feed for nursing sow according to any one of claims 8 is raised by the milking sow that method as claimed in claim 9 is prepared
Expect the application in feeding milking sow.
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| CN102934736A (en) * | 2012-12-05 | 2013-02-20 | 蒋常德 | Method for preparing sweet potato skin/ sweet potato powder dreg fermented feed |
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| JP2018007612A (en) * | 2016-07-13 | 2018-01-18 | 株式会社岡安商店 | Dietary fiber having protein saccharization suppression function and method for producing the same |
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Application publication date: 20181207 |