CN108925548A - 一种冻存细胞制剂及细胞复苏方式 - Google Patents

一种冻存细胞制剂及细胞复苏方式 Download PDF

Info

Publication number
CN108925548A
CN108925548A CN201710375424.XA CN201710375424A CN108925548A CN 108925548 A CN108925548 A CN 108925548A CN 201710375424 A CN201710375424 A CN 201710375424A CN 108925548 A CN108925548 A CN 108925548A
Authority
CN
China
Prior art keywords
cells
preparation
cell
frozen
cryopreservation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710375424.XA
Other languages
English (en)
Chinese (zh)
Inventor
何佳平
张丽
刘丽萍
林南静
刘欣
李超
王飞
余舒倩
胡永来
赵佳维
孙哲
刘晓雨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Cellular Biopharmaceutical Group Ltd
Original Assignee
Cellular Biomedicine Group HK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellular Biomedicine Group HK Ltd filed Critical Cellular Biomedicine Group HK Ltd
Priority to CN201710375424.XA priority Critical patent/CN108925548A/zh
Priority to EP18805534.7A priority patent/EP3632207A4/en
Priority to PCT/CN2018/088246 priority patent/WO2018214943A1/zh
Priority to NZ759568A priority patent/NZ759568A/en
Priority to KR1020197038096A priority patent/KR102350423B1/ko
Priority to JP2020515812A priority patent/JP7303184B2/ja
Priority to US16/616,216 priority patent/US20200170242A1/en
Priority to AU2018272688A priority patent/AU2018272688B2/en
Publication of CN108925548A publication Critical patent/CN108925548A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/31—Chimeric antigen receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/428—Undefined tumor antigens, e.g. tumor lysate or antigens targeted by cells isolated from tumor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/20—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing sulfur, e.g. dimethyl sulfoxide [DMSO], docusate, sodium lauryl sulfate or aminosulfonic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Inorganic Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
CN201710375424.XA 2017-05-24 2017-05-24 一种冻存细胞制剂及细胞复苏方式 Pending CN108925548A (zh)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CN201710375424.XA CN108925548A (zh) 2017-05-24 2017-05-24 一种冻存细胞制剂及细胞复苏方式
EP18805534.7A EP3632207A4 (en) 2017-05-24 2018-05-24 FORMULATION FOR CELL PRESERVATION AND CELL RECOVERY METHOD
PCT/CN2018/088246 WO2018214943A1 (zh) 2017-05-24 2018-05-24 一种冻存细胞制剂及细胞复苏方式
NZ759568A NZ759568A (en) 2017-05-24 2018-05-24 Cell cryopreservation formulation and cell recovery method
KR1020197038096A KR102350423B1 (ko) 2017-05-24 2018-05-24 세포 동결 보존 제제 및 세포 소생 방법
JP2020515812A JP7303184B2 (ja) 2017-05-24 2018-05-24 凍結保存細胞製剤および細胞再生方法
US16/616,216 US20200170242A1 (en) 2017-05-24 2018-05-24 Cell cryopreservation formulation and cell recovery method
AU2018272688A AU2018272688B2 (en) 2017-05-24 2018-05-24 Cell cryopreservation formulation and cell recovery method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710375424.XA CN108925548A (zh) 2017-05-24 2017-05-24 一种冻存细胞制剂及细胞复苏方式

Publications (1)

Publication Number Publication Date
CN108925548A true CN108925548A (zh) 2018-12-04

Family

ID=64396262

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710375424.XA Pending CN108925548A (zh) 2017-05-24 2017-05-24 一种冻存细胞制剂及细胞复苏方式

Country Status (8)

Country Link
US (1) US20200170242A1 (enExample)
EP (1) EP3632207A4 (enExample)
JP (1) JP7303184B2 (enExample)
KR (1) KR102350423B1 (enExample)
CN (1) CN108925548A (enExample)
AU (1) AU2018272688B2 (enExample)
NZ (1) NZ759568A (enExample)
WO (1) WO2018214943A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679588A (zh) * 2019-10-23 2020-01-14 厦门生命互联科技有限公司 一种嵌合抗原受体t细胞冻存介质及用途
CN115136955A (zh) * 2022-08-03 2022-10-04 深圳宾德生物技术有限公司 细胞冻存液及其制备方法和应用

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021245671A1 (en) * 2020-03-31 2022-10-20 Cell Exosome Therapeutics Inc. Cell preservation method
AU2021324669A1 (en) * 2020-08-10 2023-02-23 Angiocrine Bioscience, Inc. Cryopreserved endothelial cell compositions
TW202245811A (zh) * 2021-02-03 2022-12-01 美商異基因治療有限公司 用於ｃａｒ ｔ細胞藥物產品的調配物與製程
US20220354108A1 (en) * 2021-05-04 2022-11-10 Tissue Testing Technologies Llc Preservation methods using trehalose with other cryoprotectants being absent from the cryopreservation protocol
CN113994951A (zh) * 2021-11-17 2022-02-01 晋文娟 一种cik细胞冻存液及冻存方法
CN114097771B (zh) * 2021-12-15 2022-10-14 珠海贝索细胞科学技术有限公司 一种细胞保存液及其应用和细胞保存方法
CN116058363B (zh) * 2023-01-13 2025-10-28 杭州中赢生物医疗科技有限公司 一种增加nk细胞冻存复苏后活性的方法及其应用

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1575643A2 (en) * 2002-12-20 2005-09-21 Cell Genesys, Inc. Directly injectable formulations which provide enhanced cryoprotection of cell products
CN102119936A (zh) * 2011-03-07 2011-07-13 李荣旗 制备利用人羊膜间充质细胞治疗缺血性脑损伤注射液的方法及其注射液
CN102908364A (zh) * 2012-11-14 2013-02-06 青岛奥克生物开发有限公司 一种间充质干细胞注射液及其制备方法和在制备治疗儿童扩张型心肌病药物中的应用
CN104857022A (zh) * 2015-05-21 2015-08-26 北京青藤谷禧干细胞科技研究院有限公司 一种间充质干细胞注射液、制备方法及其应用
CN104873542A (zh) * 2015-05-21 2015-09-02 北京青藤谷禧干细胞科技研究院有限公司 一种脐带间充质干细胞注射液及其制备方法和应用
CN105325402A (zh) * 2015-11-13 2016-02-17 黄林海 一种用于玻璃化冻存骨髓间充质干细胞的冻存保护剂
CN105394026A (zh) * 2015-10-31 2016-03-16 江苏赛尔特生物技术有限公司 一种造血干细胞冻存新方法
CN105660606A (zh) * 2016-03-10 2016-06-15 广州赛莱拉干细胞科技股份有限公司 一种细胞冻存液
CN106177918A (zh) * 2016-09-30 2016-12-07 广州赛莱拉干细胞科技股份有限公司 一种间充质干细胞注射液及其制备方法和应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6713245B2 (en) * 1998-07-06 2004-03-30 Diacrin, Inc. Methods for storing neural cells such that they are suitable for transplantation
US20030054331A1 (en) * 2001-09-14 2003-03-20 Stemsource, Inc. Preservation of non embryonic cells from non hematopoietic tissues
US7270946B2 (en) * 2002-10-04 2007-09-18 Organ Recovery Systems, Inc. Method for treatment of cellular materials with sugars prior to preservation
US20070025961A1 (en) 2003-06-03 2007-02-01 Kenzo Bamba Composition for stabilizing survival of transplanted hematopoietic stem cell, kit for obtaining the composition, method of stabilizing survival of transplanted hematopoietic stem cell, human monoclonal antibody or human polyclonal antibody and method of producing the same, gene encoding human monoclonal antibody and transf
CN103461322B (zh) * 2012-06-07 2016-08-03 西比曼生物科技（上海）有限公司 用于冻存复苏后的细胞的保存液
CN102907416B (zh) * 2012-08-01 2016-08-03 西比曼生物科技（上海）有限公司 一种可维持细胞活性的组织冻存液
CN102792947B (zh) * 2012-09-03 2014-03-26 四川新生命干细胞科技股份有限公司 一种间充质干细胞冻存液及注射液
EP3066193B1 (en) 2013-11-04 2024-07-10 Isopogen Pty Ltd Cell culture method
CN114540266A (zh) 2014-07-07 2022-05-27 塔尔加泽梅股份有限公司 用于治疗用途的岩藻糖基化细胞的制备和冷冻干燥
CN104430301A (zh) * 2014-11-18 2015-03-25 成都康华生物制品有限公司 一种提高人二倍体细胞存活率的方法
US20180116956A1 (en) * 2015-04-02 2018-05-03 Stegi-Ra Trust Composition for use in treating celiac disease
CN105477017A (zh) * 2015-12-03 2016-04-13 深圳爱生再生医学科技有限公司 治疗糖尿病足的混合干细胞制剂及其制备方法
US11666649B2 (en) * 2016-10-11 2023-06-06 University Of Miami Vectors and vaccine cells for immunity against Zika virus
CN106665560B (zh) * 2017-01-16 2021-02-05 哈尔滨医科大学 一种直接静脉回输的免疫细胞冻存液及其应用

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1575643A2 (en) * 2002-12-20 2005-09-21 Cell Genesys, Inc. Directly injectable formulations which provide enhanced cryoprotection of cell products
CN102119936A (zh) * 2011-03-07 2011-07-13 李荣旗 制备利用人羊膜间充质细胞治疗缺血性脑损伤注射液的方法及其注射液
CN102908364A (zh) * 2012-11-14 2013-02-06 青岛奥克生物开发有限公司 一种间充质干细胞注射液及其制备方法和在制备治疗儿童扩张型心肌病药物中的应用
CN104857022A (zh) * 2015-05-21 2015-08-26 北京青藤谷禧干细胞科技研究院有限公司 一种间充质干细胞注射液、制备方法及其应用
CN104873542A (zh) * 2015-05-21 2015-09-02 北京青藤谷禧干细胞科技研究院有限公司 一种脐带间充质干细胞注射液及其制备方法和应用
CN105394026A (zh) * 2015-10-31 2016-03-16 江苏赛尔特生物技术有限公司 一种造血干细胞冻存新方法
CN105325402A (zh) * 2015-11-13 2016-02-17 黄林海 一种用于玻璃化冻存骨髓间充质干细胞的冻存保护剂
CN105660606A (zh) * 2016-03-10 2016-06-15 广州赛莱拉干细胞科技股份有限公司 一种细胞冻存液
CN106177918A (zh) * 2016-09-30 2016-12-07 广州赛莱拉干细胞科技股份有限公司 一种间充质干细胞注射液及其制备方法和应用

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679588A (zh) * 2019-10-23 2020-01-14 厦门生命互联科技有限公司 一种嵌合抗原受体t细胞冻存介质及用途
CN115136955A (zh) * 2022-08-03 2022-10-04 深圳宾德生物技术有限公司 细胞冻存液及其制备方法和应用

Also Published As

Publication number Publication date
KR102350423B1 (ko) 2022-01-11
WO2018214943A1 (zh) 2018-11-29
US20200170242A1 (en) 2020-06-04
EP3632207A4 (en) 2021-03-10
KR20200073171A (ko) 2020-06-23
AU2018272688B2 (en) 2021-05-27
JP2020520680A (ja) 2020-07-16
NZ759568A (en) 2023-01-27
AU2018272688A1 (en) 2019-12-19
JP7303184B2 (ja) 2023-07-04
EP3632207A1 (en) 2020-04-08

Similar Documents

Publication Publication Date Title
CN108925548A (zh) 一种冻存细胞制剂及细胞复苏方式
CN110050782A (zh) 一种干细胞冻存液及其制备方法和冻存方法
CN110959606B (zh) 一种免疫效应细胞冻存液及其用途
CN105994254A (zh) 一种dc细胞的冻存液及冻存方法
CN109315386A (zh) 一种可用于造血干细胞或淋巴细胞的冻存液及冻存方法
WO2016076428A1 (ja) 臍帯血および末梢血の凍結保存方法および凍結保存用溶液
BR112018077047B1 (pt) Cassete e automato para cultura celular
CN102559581B (zh) 一种无血清肝细胞培养基
CN109619088A (zh) 一种脂肪间充质干细胞的保存液
WO2021190620A1 (zh) 细胞保存液及其试剂盒
CN114391536B (zh) 一种间充质干细胞冻存液
CN114403127B (zh) 一种间充质干细胞冷藏保护液及保存方法
Tianhang et al. Autologous transplantation of endothelial progenitor cells to prevent multiple organ dysfunction syndromes in pig
CN107912425B (zh) 一种可输液的细胞冻存管试剂盒及其应用
Kaiser et al. Lentiviral vector-mediated gene therapy of hepatocytes ex vivo for autologous transplantation in swine
CN207121593U (zh) 一种冻存细胞试剂盒
CN114763527A (zh) 一种高效稳定工作细胞库的制备方法
CN118680163A (zh) 一种脐带间充质干细胞注射液冻存液、制备方法及应用
Scharp et al. Advances in human islet processing: manufacturing steps to achieve predictable islet outcomes from research pancreases
HK40017286A (en) Cell cryopreservation formulation and cell recovery method
CN110882276B (zh) 细胞治疗组合物及治疗血管病变的方法
CN106867877A (zh) 一种密闭式慢病毒载体培养装置和培养方法
CN116584479B (zh) 一种细胞冷藏保护液及其制备方法和应用
NL2034495B1 (en) Stem cell cryopreservation protective agent, preparation method, and application thereof
CN117859733A (zh) 一种牙髓间充质干细胞保存液及其使用方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20221021

Address after: Floor 6, Building 1, No. 333 Guiping Road, Xuhui District, Shanghai

Applicant after: CELLULAR BIOMEDICINE GROUP (SHANGHAI) Ltd.

Address before: Hongkong Jinzhong Chinese Cotton Tree Drive Fairmont House No. 8 Building 402 room 4

Applicant before: CELLULAR BIOMEDICINE GROUP HK Ltd.

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20221221

Address after: 201210 Building 3, No. 85 Faraday Road, Pudong New Area (Shanghai) Pilot Free Trade Zone, Shanghai

Applicant after: SHANGHAI CELLULAR BIOPHARMACEUTICAL Group Ltd.

Address before: 200233 6th floor, building 1, 333 Guiping Road, Xuhui District, Shanghai

Applicant before: CELLULAR BIOMEDICINE GROUP (SHANGHAI) Ltd.