CN108913124B - 一种壳聚糖衍生物p在检测铝离子中的应用 - Google Patents

一种壳聚糖衍生物p在检测铝离子中的应用 Download PDF

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CN108913124B
CN108913124B CN201810818779.6A CN201810818779A CN108913124B CN 108913124 B CN108913124 B CN 108913124B CN 201810818779 A CN201810818779 A CN 201810818779A CN 108913124 B CN108913124 B CN 108913124B
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chitosan derivative
aluminum ions
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张军
于春伟
季玉祥
傅琼瑶
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Abstract

本发明涉及对铝离子的检测，具体讲是一种壳聚糖衍生物P在检测铝离子中的应用。一种壳聚糖衍生物P在检测铝离子中的应用。所述壳聚糖衍生物P可作为荧光探针实现对铝离子的识别检测。本发明通过有效的合成手段得到的壳聚糖衍生物P，对铝离子表现出较好的选择性，可以实现对铝离子的检测。

Description

一种壳聚糖衍生物P在检测铝离子中的应用
技术领域
本发明涉及对铝离子的检测，具体讲是一种壳聚糖衍生物P在检测铝离子中的应用。
背景技术
大多数报道的荧光探针是在发光团上修饰的生物小分子，具有生物毒性较大，水溶性低等缺点[1-3]，这成为荧光探针研究的限制因素。而用壳聚糖及其衍生物做载体，辅助合成的荧光探针可以很好的解决水溶性和生物毒性的问题。基于壳聚糖衍生物的荧光探针已被应用到汞离子、铜离子的检测中[3]。但在对铝离子检测方面的报道较少。
发明内容
本发明的目的是提供一种壳聚糖衍生物P在检测铝离子中的应用。
为实现上述目的，本发明采用技术方案为：
一种壳聚糖衍生物P在检测铝离子中的应用。
所述壳聚糖衍生物P在作为铝离子荧光探针的应用。
所述壳聚糖衍生物P如式1：
Figure BDA0001740907900000011
所述壳聚糖衍生物P如式1的制备：将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中，加热回流4h，冷却至室温后，倒入冷的丙酮溶液中，析出固体过滤，干燥后得淡黄色固体纯品P。
进一步的说：首先将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中，80℃下以摩尔比1︰1反应4h，冷却后将反应液倒入冷的丙酮溶液中，将析出固体抽滤，干燥，得淡黄色固体纯品P。
本发明所具有的优点：本发明将合成的壳聚糖衍生物P作为荧光探针实现对铝离子的检测。该探针具有选择性好、水溶性高、灵敏度高、生物毒性低等优点。
附图说明
图1为本发明采用的壳聚糖衍生物P的红外谱图。
图2为本发明采用的壳聚糖衍生物P的核磁谱图。
图3为本发明是实例提供的壳聚糖衍生物P与铝离子的荧光滴定光谱图，插图为壳聚糖衍生物P对铝离子响应的曲线；其中，乙醇-水溶液，v/v，1:4，20mM HEPES,pH 10.0；铝离子浓度范围为：0—50.0μM。
具体实施方式
下面结合附图和实施例对本发明做进一步的解释说明。
实施例1
壳聚糖衍生物P的制备，下述为壳聚糖衍生物P的合成路线：
Figure BDA0001740907900000021
将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中，80℃下以摩尔比1︰1反应4h，冷却后将反应液倒入冷的丙酮溶液中，析出固体抽滤，干燥，得淡黄色固体纯品P，产率85％(参见图1和2)。
从图1的红外吸收和图2中H的化学位移可知壳聚糖衍生物P的生成。
实施例2
壳聚糖衍生物P对铝离子的光学识别
1)条件的选择：在乙醇/水(1/4，v/v)介质中，用HEPES缓冲溶液控制体系pH为6.0。
2)实验方法：5mL比色管中，加入不同浓度的铝离子标准溶液，后加入50μL含1mM探针分子P(壳聚糖衍生物P)的DMSO标准溶液，以乙醇/水(1/4,v:v,pH6.0,20mM HEPES)稀释至刻度，摇匀。测试时，激发/发射狭缝＝10/10nm，激发波长为340nm(参见图3)。
由图3可见，铝离子的荧光滴定谱图光学性质测定探针分子P(50μM)的乙醇/水(1/4,v:v,pH6.0,20mM HEPES)溶液，荧光很弱，说明体系中探针分子P自身无荧光；加入铝离子后，447nm处出现荧光峰，说明探针分子P与铝离子络合后，产生强荧光，并且随着铝离子浓度的增加，447nm处的荧光强度随之增强。在乙醇/水(1/4,v:v,pH6.0,20mM HEPES)溶液中，探针分子P对铝离子具有较好的选择性，得到探针分子P(50μM)对铝离子响应的曲线，线性范围为0.9-4.0μM，检出限为0.3μM。
参考文献：
1)Y.T.Li and C.M.Yang,Chem.Commun.,2003,2884-2885.
2)Q.T.Meng,C.He,W.P.Su,Sens.Actuators,B.Chem.,2012,312-317.
3)M.N.Elizabeth,J.L.Stephen,J.Mater.Chem.,2005,15,2778-2783.

Claims (3)

1.一种壳聚糖衍生物P在检测铝离子中的应用；
所述壳聚糖衍生物P如式1：
Figure FDA0002643580070000011
2.按权利要求1所述的壳聚糖衍生物P在检测铝离子中的应用，其特征在于：所述壳聚糖衍生物P在作为铝离子荧光探针的应用。
3.按权利要求2所述的壳聚糖衍生物P在检测铝离子中的应用，其特征在于：将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中，加热回流4h，冷却至室温后，倒入冷的丙酮溶液中，析出固体过滤，干燥后得淡黄色固体纯品P。
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