CN108913124A - 一种壳聚糖衍生物p在检测铝离子中的应用 - Google Patents
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Abstract
本发明涉及对铝离子的检测,具体讲是一种壳聚糖衍生物P在检测铝离子中的应用。一种壳聚糖衍生物P在检测铝离子中的应用。所述壳聚糖衍生物P可作为荧光探针实现对铝离子的识别检测。本发明通过有效的合成手段得到的壳聚糖衍生物P,对铝离子表现出较好的选择性,可以实现对铝离子的检测。
Description
技术领域
本发明涉及对铝离子的检测,具体讲是一种壳聚糖衍生物P在检测铝离子中的应用。
背景技术
大多数报道的荧光探针是在发光团上修饰的生物小分子,具有生物毒性较大,水溶性低等缺点[1-3],这成为荧光探针研究的限制因素。而用壳聚糖及其衍生物做载体,辅助合成的荧光探针可以很好的解决水溶性和生物毒性的问题。基于壳聚糖衍生物的荧光探针已被应用到汞离子、铜离子的检测中[3]。但在对铝离子检测方面的报道较少。
发明内容
本发明的目的是提供一种壳聚糖衍生物P在检测铝离子中的应用。
为实现上述目的,本发明采用技术方案为:
一种壳聚糖衍生物P在检测铝离子中的应用。
所述壳聚糖衍生物P在作为铝离子荧光探针的应用。
所述壳聚糖衍生物P如式1:
所述壳聚糖衍生物P如式1的制备:将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中,加热回流4h,冷却至室温后,倒入冷的丙酮溶液中,析出固体过滤,干燥后得淡黄色固体纯品P。
进一步的说:首先将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中,80℃下以摩尔比1︰1反应4h,冷却后将反应液倒入冷的丙酮溶液中,将析出固体抽滤,干燥,得淡黄色固体纯品P。
本发明所具有的优点:本发明将合成的壳聚糖衍生物P作为荧光探针实现对铝离子的检测。该探针具有选择性好、水溶性高、灵敏度高、生物毒性低等优点。
附图说明
图1为本发明采用的壳聚糖衍生物P的红外谱图。
图2为本发明采用的壳聚糖衍生物P的核磁谱图。
图3为本发明是实例提供的壳聚糖衍生物P与铝离子的荧光滴定光谱图,插图为壳聚糖衍生物P对铝离子响应的曲线;其中,乙醇-水溶液,v/v,1:4,20mM HEPES,pH 10.0;铝离子浓度范围为:0—50.0μM。
具体实施方式
下面结合附图和实施例对本发明做进一步的解释说明。
实施例1
壳聚糖衍生物P的制备,下述为壳聚糖衍生物P的合成路线:
将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中,80℃下以摩尔比1︰1反应4h,冷却后将反应液倒入冷的丙酮溶液中,析出固体抽滤,干燥,得淡黄色固体纯品P,产率85%(参见图1和2)。
从图1的红外吸收和图2中H的化学位移可知壳聚糖衍生物P的生成。
实施例2
壳聚糖衍生物P对铝离子的光学识别
1)条件的选择:在乙醇/水(1/4,v/v)介质中,用HEPES缓冲溶液控制体系pH为6.0。
2)实验方法:5mL比色管中,加入不同浓度的铝离子标准溶液,后加入50μL含1mM探针分子P(壳聚糖衍生物P)的DMSO标准溶液,以乙醇/水(1/4,v:v,pH6.0,20mM HEPES)稀释至刻度,摇匀。测试时,激发/发射狭缝=10/10nm,激发波长为340nm(参见图3)。
由图3可见,铝离子的荧光滴定谱图光学性质测定探针分子P(50μM)的乙醇/水(1/4,v:v,pH6.0,20mM HEPES)溶液,荧光很弱,说明体系中探针分子P自身无荧光;加入铝离子后,447nm处出现荧光峰,说明探针分子P与铝离子络合后,产生强荧光,并且随着铝离子浓度的增加,447nm处的荧光强度随之增强。在乙醇/水(1/4,v:v,pH6.0,20mM HEPES)溶液中,探针分子P对铝离子具有较好的选择性,得到探针分子P(50μM)对铝离子响应的曲线,线性范围为0.9-4.0μM,检出限为0.3μM。
参考文献:
1)Y.T.Li and C.M.Yang,Chem.Commun.,2003,2884-2885.
2)Q.T.Meng,C.He,W.P.Su,Sens.Actuators,B.Chem.,2012,312-317.
3)M.N.Elizabeth,J.L.Stephen,J.Mater.Chem.,2005,15,2778-2783.
Claims (4)
1.一种壳聚糖衍生物P在检测铝离子中的应用。
2.按权利要求1所述的壳聚糖衍生物P在检测铝离子中的应用,其特征在于:所述壳聚糖衍生物P在作为铝离子荧光探针的应用。
3.按权利要求1或2所述的壳聚糖衍生物P在检测铝离子中的应用,其特征在于:所述壳聚糖衍生物P如式1:
4.按权利要求3所述的壳聚糖衍生物P在检测铝离子中的应用,其特征在于:将羧基化壳聚糖与邻羟基萘甲醛在无水乙醇中,加热回流4h,冷却至室温后,倒入冷的丙酮溶液中,析出固体过滤,干燥后得淡黄色固体纯品P。
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CN110128566B (zh) * | 2019-04-30 | 2021-09-07 | 盐城工学院 | 一种识别甲醛的近红外荧光聚合物探针及其制备方法和应用 |
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