CN108904394B - Traditional Chinese medicine composition and preparation method thereof - Google Patents
Traditional Chinese medicine composition and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a Chinese medicinal composition and a preparation method thereof, wherein the composition comprises ligusticum wallichii, safflower, poria cocos, bighead atractylodes rhizome, astragalus mongholicus and gentian. The composition starts from the principle of monarch, minister, assistant and guide of Chinese medicine compatibility, has synergistic effect from four angles of 'invigorating, benefiting, tonifying and clearing', and has obvious effect on black eye, pouch and edema aiming at various reasons causing eye skin problems. The invention also discloses an extraction method for preparing the traditional Chinese medicine composition, which is simple and efficient, and has high extraction efficiency and short extraction time. The invention also discloses an eye skin care product containing the traditional Chinese medicine composition.
Description
Technical Field
The invention relates to the field of cosmetics, and in particular relates to a traditional Chinese medicine composition for fading dark eye circles, under-eye puffiness and edema, a preparation method and application thereof.
Background
The eye skin is the thinnest part of human skin, and the thickness of the epidermis and the dermis is only 0.25-0.55 mm, so the eye skin is more easily influenced by the external environment and the factors in the body. The skin of the eye part lacks sebaceous glands and sweat glands, can supply relatively less grease, has no natural moistening capacity, and is more prone to dryness and lack of moisture than the skin of other parts. The subcutaneous loose connective tissue is rich and is easy to be filled with blood and water, and the fiber structure of the loose connective tissue is distributed with superfine and fragile capillary vessels and lymphatic systems which are sensitive and fragile and are easy to be damaged and injured to cause local blood blockage and edema. Meanwhile, the collagen and the elastic fibers are rarely distributed, and the collagen and the elastic fibers are lost with the increase of age, the skin around the eyes becomes more relaxed to form lower eyelid edema, the lymphatic circulation and the blood circulation become slower, and the backflow of blood and body fluid is insufficient, so that pigment and effusion are deposited on connective tissues to form black eyes, edema and eye bags.
The dark eye circles around eyes are caused by frequent staying up, unstable emotion, eye fatigue, aging, too slow blood flow of venous blood vessels, insufficient supply of oxygen to erythrocyte cells, excessive accumulation of carbon dioxide and metabolic wastes in the venous blood vessels, formation of chronic hypoxia, dark blood and formation of stagnation and pigmentation of eyes. The older the person, the thinner the subcutaneous fat around the eye becomes, so the more visible the dark circles.
The eye edema is caused by poor sleep at night, damaged skin of eyes, blood backflow problem, capillary vessel seepage caused by inflammation and accumulation under eyes. The traditional Chinese medicine explanation is as follows: edema caused by water-dampness retention due to qi and blood stagnation, edema caused by water-dampness accumulation when qi fails to operate, and accumulation of stagnant fluid in the skin of the eyes to form edema of the eyes.
Under-eye puffiness is a bag which is formed by that the skin of the eye is aged and damaged, the microcirculation is poor for a long time, water and fat are accumulated due to obstruction, the skin around the eyelid is reduced in elasticity and is loose, the orbicularis oculi is loose, and the accumulated fat and effusion move down on the lower orbit due to the action of gravity to form a bulge.
The eye skin care products or functional substances on the market at present solve the eye skin problems from the aspects of moisturizing, whitening, repairing, eliminating inflammatory reaction and the like, and aim at the formed appearance, so that the symptoms are treated and the root causes are not treated. For eye problems such as black eye circles, eye bags, eye edema and the like, the prior art generally starts with a single problem and aims at improving one symptom.
The eutectic solvent is a novel green solvent which is a hot spot in recent years and replaces a traditional solvent, and is a solvent which is formed by combining a hydrogen bond acceptor (choline chloride, betaine and the like) and a hydrogen bond donor (polyalcohol, carboxylic acids and the like) through hydrogen bonds, and the freezing point of the eutectic solvent is obviously lower than the melting point of each component. The eutectic solvent extraction has the advantages of extracting not only water-soluble active ingredients in plants, such as polysaccharides and organic acids, but also oil-soluble active ingredients in plants, such as flavonoids and saponins, and also extracting active ingredients with poor water and oil solubility, such as tannins. Therefore, the use of the eutectic solvent is more advantageous for the extraction of the effective ingredients of the Chinese medicine in view of the extraction efficiency and the complexity of the components of the Chinese medicine. In addition, the raw materials of the eutectic solvent are common compounds, and the eutectic solvent is rich in source and low in cost, so that the extraction method of the eutectic solvent is expected to realize industrial production.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition with the effects of fading dark eye circles, under-eye puffiness and edema.
The invention also aims to provide an extraction method of the active ingredients of the traditional Chinese medicine composition.
The invention also aims to provide an eye skin care product prepared by using the traditional Chinese medicine composition.
The invention provides a traditional Chinese medicine composition for eye care, which can lighten dark eye circles, eye bags and eye edema starting from the internal cause of eye problems.
The traditional Chinese medicine composition adopts the monarch, minister, assistant and guide principles of traditional Chinese medicine compatibility, has the effects of promoting blood circulation to remove blood stasis, inducing diuresis to alleviate edema, tonifying qi and consolidating superficial resistance, clearing heat and drying dampness, and solves the internal causes of black eye, pouch and eye edema in symptom-free manner.
TABLE 1 compositions and respective efficacy of the Chinese medicinal composition of the present invention
The medicines composing the prescription can be divided into monarch, ministerial, adjuvant and conductant medicines according to the functions of the medicines in the prescription, and the medicines are called monarch, ministerial, adjuvant and conductant medicines. Jun means herbs in the prescription that have main therapeutic effects on principal syndrome, one or more herbs can be used as required. Ministerial refers to the drugs that assist monarch drugs in treating principal symptoms or main concurrent symptoms. The adjuvant is a medicine which combines the treatment of both monarch and ministerial medicines, or inhibits the toxicity of the monarch and ministerial medicines, or plays an anti-adjuvant role. So that the fingers can direct the herbs to the affected part or harmonize the herbs. The original Li Gao written in the theory of spleen and stomach: the amount of monarch drugs is the most, the ministerial drugs are the second to the ministerial drugs, and the conductant drugs are the second to the ministerial drugs; the monarch and minister medicines are orderly and mutually diffused, so that the medicine can defend the evil and eliminate the disease . The main symptoms of formation of the black eye, the pouch and the edema are qi and blood stasis, and the symptoms are water-dampness accumulation, so that the ligusticum wallichii and the safflower for activating blood and dissolving stasis are monarch drugs, the poria cocos and the bighead atractylodes rhizome for inducing diuresis and reducing edema are ministerial drugs, the astragalus mongholicus for tonifying qi and strengthening exterior and strengthening the principal and ministerial drugs is an adjuvant drug, and the gentian root for harmonizing the drugs is a conductant drug.
Rhizoma Ligustici Chuanxiong has effects of promoting blood circulation, activating qi-flowing, dispelling pathogenic wind and relieving pain. Chuan Xiong is a qi-flowing medicine in blood, so it can disperse pungent qi, relieve depression, and dredge qi, and has the functions of improving microcirculation and dilating blood vessels.
The safflower has the effects of promoting blood circulation, removing blood stasis, dissipating dampness, removing swelling and resisting blood coagulation, expands branch circulation, increases blood flow, improves the hypoxia tolerance of organisms, and has obvious inhibiting effect on the increase of the permeation quantity of skin capillaries caused by histamine.
Poria has effects of eliminating dampness, promoting diuresis, invigorating spleen, regulating stomach function, calming heart, and tranquilizing mind. The tuckahoe has the functions of promoting diuresis and excreting dampness and invigorating spleen, has obvious effect on the edema of water dampness which can not be transported and dissolved due to spleen deficiency, and promotes diuresis without hurting healthy qi. For cold-damp syndrome, it is combined with Gui Zhi and Bai Zhu, or for damp-heat syndrome, it is combined with Zhu Ling and ze Xie, etc.; for spleen qi deficiency, it is combined with Dang Shen, Huang Qi and Bai Zhu; for deficiency-cold, it is combined with Fu Zi and Bai Zhu, etc.
The rhizoma atractylodis macrocephalae has the effects of strengthening spleen and tonifying qi, eliminating dampness and promoting diuresis, resisting oxidation and resisting coagulation.
Radix astragali has effects of invigorating lung and spleen, invigorating qi and consolidating superficial resistance, dispelling pathogenic wind and removing toxic substance, and can be used for treating qi deficiency, debilitation, collapse of middle-warmer energy, and sallow complexion due to blood deficiency. Used in cosmetics, has effects of resisting oxidation, prolonging cell life, increasing SOD activity, reducing damage of lipid peroxide to biological membrane, and delaying skin aging.
Gentiana scabra Bunge, bitter in taste and cold in nature, with roots and rhizomes as raw material. The main effects are clearing heat and drying dampness, and the action of purging excessive fire of liver and gallbladder is strong, and it can extinguish wind and alleviate pain. When the guiding is used as an adjuvant, all the fire can be cured.
In order to realize the purpose of the invention, the technical scheme of the invention is as follows:
a traditional Chinese medicine composition comprises the following components in parts by weight: 15-35 parts of ligusticum wallichii, 13-32 parts of safflower, 9-24 parts of poria cocos, 8-20 parts of bighead atractylodes rhizome, 5-15 parts of astragalus membranaceus and 3-15 parts of gentian.
A preparation method of the traditional Chinese medicine composition comprises the following specific steps:
(1) the traditional Chinese medicines are ground according to the weight part ratio and then soaked in a eutectic solvent, and a liquid-solid mixture is obtained after the mixture is placed in a closed manner;
(2) heating the liquid-solid mixture prepared in the step (1), and performing ultrasonic and microwave assisted extraction to prepare an extracting solution;
(3) filtering the extracting solution prepared in the step (2) by using a filter screen, enriching and separating by using adsorption resin, eluting, collecting eluent and filtering.
The granularity of the crushed traditional Chinese medicine in the step (1) is 6-200 meshes; preferably 20-100 meshes; more preferably 40 to 80 mesh.
The eutectic solvent in the step (1) is a mixture of one of choline chloride or betaine and polyhydric alcohols; preferably choline chloride and glycerol; more preferably, the molar ratio of choline chloride to glycerol is 1: (1-3).
The water content of the eutectic solvent in the step (1) is 5-50%; preferably 10-45%; more preferably 25 to 35%.
The liquid-solid ratio of the eutectic solvent in the step (1) to the traditional Chinese medicine mixture powder is 5-20 m L/g, and preferably 12-17 m L/g.
The closed standing time in the step (1) is 1-72 h; preferably 8-48 h; further preferably 20 to 28 hours.
The heating temperature in the step (2) is 40-70 ℃; preferably 60 deg.c.
The ultrasonic power in the step (2) is 200-400W; preferably 350W.
The microwave power in the step (2) is 500-750W; preferably 600W.
The extraction time in the step (2) is 5-120 min; preferably 20 min.
The filter screen in the step (3) is 80-300 meshes; preferably 200 mesh.
The adsorption resin in the step (3) is macroporous adsorption resin; preferably AB-8 type, X-5 type, D101 type; more preferably AB-8 type.
The eluent in the step (3) is any one or the combination of at least two of ethanol, water or butanediol; preferably a combination of butanediol and water; more preferably, the mass fraction of the butanediol is 50-90%; most preferably 80%.
The traditional Chinese medicine extract prepared by the method is applied to preparing an eye skin care product with the effects of lightening dark circles, under-eye puffiness and edema.
The formula of the eye skin care product containing the traditional Chinese medicine composition comprises the following components in percentage by weight:
the traditional Chinese medicine composition is prepared according to the method, and the addition amount of the traditional Chinese medicine composition is 0.5-20%; preferably 3-10%; further preferably 5%.
A method of making the ocular skin care product, the method comprising the steps of:
(1) preparing raw materials according to the composition;
(2) weighing the phase A, sequentially adding the phase A into an emulsifying pot, heating to 85-90 ℃, homogenizing for 5 minutes, stirring, and keeping the temperature for half an hour;
(3) weighing the components of the phase B, sequentially adding the components into an oil pan, heating to 85-90 ℃, and uniformly stirring;
(4) slowly adding the material in the oil pan into an emulsifying pan under stirring, homogenizing for 5 minutes, vacuumizing, preserving heat, stirring for 20 minutes, and then starting cooling water;
(5) and (4) when the temperature of the mixture in the step (4) is reduced to 40 ℃, sequentially adding the components of the phase C and the phase D, vacuumizing and uniformly stirring, reducing the temperature to 38 ℃, and discharging.
The invention has the following beneficial effects:
(1) the traditional Chinese medicine composition adopts the monarch, minister, assistant and guide principles of traditional Chinese medicine compatibility, has the effects of promoting blood circulation to remove blood stasis, inducing diuresis to alleviate edema, tonifying qi and consolidating superficial resistance, clearing heat and drying dampness, and solves the internal causes of black eye, pouch and eye edema in multiple ways and has obvious effect;
(2) the traditional Chinese medicine composition disclosed by the invention has the advantages that the six components of ligusticum wallichii, safflower, poria cocos, bighead atractylodes rhizome, astragalus mongholicus and gentian are mutually promoted and synergized, so that the effect of remarkably fading black eyes, pouches and edema is achieved, and the traditional Chinese medicine composition is safe and non-irritant and can be used as a raw material of a skin care product;
(3) the method adopts the eutectic solvent to extract the effective components of the traditional Chinese medicine, has simple operation, high extraction efficiency, short time consumption and low cost, and is beneficial to industrial production; meanwhile, the eutectic solvent used for extraction can be repeatedly used, and the economic benefit is high after multiple extractions.
Drawings
FIG. 1 shows the difference in the rate of change of the pigment value of the eye skin between examples 3 to 6 and commercial eye creams and the control
FIG. 2 the difference change rates of the skin pigment values of the eyes of example 6 and comparative examples 1 to 6
FIG. 3 the difference change rates of the skin pigment values of the eyes of example 6 and comparative examples 7 to 12
FIG. 4 values of changes in skin blood flow values of lower eyelid portions in example 6, commercial eye cream, and comparative examples 7 and 8
The specific implementation scheme is as follows:
in order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail with reference to examples and comparative examples. The specific examples do not represent limitations to the scope of the invention. Insubstantial modifications and adaptations of the present invention by others of the concepts fall within the scope of the invention.
Example 1 extraction of active substances of Chinese medicinal composition with different kinds and ratios of eutectic solvents
Weighing the traditional Chinese medicine composition with the total weight of 1 kg according to the weight parts shown in the table 2.
TABLE 2
Extracting the active substances of the traditional Chinese medicine according to the following steps:
(1) the eutectic solvent mixture and deionized water were mixed and dissolved in a mass ratio of 7:3 to prepare a eutectic solvent.
(2) Mixing the traditional Chinese medicine components in parts by weight, crushing into 40 meshes, wetting and soaking by using the eutectic solvent prepared in the step (1), sealing and standing for 22 hours, wherein the liquid-solid ratio of the solvent dosage to the traditional Chinese medicine composition weight is 12m L/g, and preparing a liquid-solid mixture.
(3) Extracting the liquid-solid mixture prepared in the step (2) for 120min at the temperature of 50 ℃, the auxiliary ultrasonic power of 200W and the auxiliary microwave power of 750W to prepare an extracting solution;
(4) filtering the extracting solution prepared in the step (3) by using a 200-mesh filter screen, enriching and separating by using AB-8 type macroporous adsorption resin, eluting by using 80% butanediol, collecting eluent and filtering.
Measuring the content of flavonoids in the extract by using an Evolution 220 ultraviolet spectrophotometer of the U.S. Thermo company, measuring the content of tannins in the extract by using a 1260Infinity high performance liquid chromatograph of the U.S. Agilent company, and measuring the content of saponins in the extract by using an X-treme L C ultrahigh pressure liquid chromatograph of the Japan Jasco company, wherein the specific contents are shown in the following table 3 (mass percent):
TABLE 3 content of active substances of Chinese medicinal composition extracted from eutectic solvent of different kinds and ratios
| Group of | Solvent composition and molar ratio | Content of flavonoids | Content of tannins | Saponins content |
| a | Betaine/glycerol (1: 3) | 4.71 | 6.86 | 3.14 |
| b | Betaine/glycerol (1: 1) | 4.58 | 6.65 | 3.01 |
| c | Betaine/1, 2 hexanediol (1: 3) | 4.82 | 5.75 | 3.36 |
| d | Betaine/1, 2 hexanediol (1: 1) | 4.15 | 5.28 | 3.53 |
| e | Betaine/ethylene glycol (1: 3) | 3.22 | 5.38 | 2.81 |
| f | Betaine/ethylene glycol (1: 1) | 3.57 | 5.67 | 2.92 |
| g | Choline chloride/glycerol (1: 3) | 5.48 | 8.28 | 3.94 |
| g | Choline chloride/glycerol (1: 1) | 4.56 | 6.37 | 2.84 |
| i | Choline chloride/1, 2 hexanediol (1: 3) | 4.72 | 5.86 | 2.98 |
| j | Choline chloride/1, 2 hexanediol (1: 1) | 4.17 | 5.31 | 3.16 |
| k | Choline chloride/ethylene glycol (1: 3) | 4.69 | 5.88 | 2.54 |
| l | Choline chloride/ethylene glycol (1: 1) | 4.97 | 5.12 | 2.62 |
As can be seen from the results in Table 3, the eutectic solvent composed of either choline chloride or betaine and polyhydric alcohol in a certain ratio has good extraction ability for the active substances of the Chinese medicinal composition. When the eutectic solvent is choline chloride and glycerol (groups g and h), the extraction effect of the three active substances of the traditional Chinese medicine composition is better than that of other eutectic solvents. When the molar ratio of choline chloride to glycerol in the eutectic solvent is 1: when 3 (group g), the extraction effect on the three active substances was the best.
Example 2 comparison of the Effect of the method for extracting eutectic solvent of the invention and the conventional method for extracting solvent
Weighing a traditional Chinese medicine mixture with the total weight of 1 kg according to the weight part ratio of the traditional Chinese medicines in the embodiment 1, taking the active substance of the traditional Chinese medicine composition extracted by a traditional water decoction method as a sample m, and taking the active substance of the traditional Chinese medicine composition extracted by an ethanol reflux method as a sample n.
The water decoction method comprises mixing the above Chinese medicinal materials in parts by weight, pulverizing into 60 mesh, soaking in distilled water, sealing, standing for 48 hr, wherein the liquid-solid ratio of the distilled water to the Chinese medicinal composition is 20m L/g, decocting at normal pressure for 90min for 3 times, standing the decoction at room temperature for 2 hr, filtering with 200 mesh filter screen, enriching and separating with AB-8 type macroporous adsorbent resin, eluting with 80% butanediol, collecting eluate, and filtering;
the ethanol reflux method comprises the specific steps of mixing the components of the traditional Chinese medicine composition according to parts by weight, crushing the mixture into 60 meshes, wetting and soaking the mixture by using an ethanol water solution with the mass fraction of 80%, placing the mixture in a closed state for 24 hours, carrying out reflux extraction for 60 minutes at normal pressure and the temperature of 70 ℃, wherein the liquid-solid ratio of the solvent dosage to the weight of the traditional Chinese medicine composition is 12m L/g, carrying out reflux extraction for 2 times at the temperature, standing the extracting solution for 2 hours at normal temperature, filtering the extracting solution by using a 200-mesh filter screen, carrying out enrichment separation by using AB-8 type macroporous adsorption resin, eluting the obtained product by using 80% butanediol.
Measuring the content of flavonoids in the sample with an Evolution 220 ultraviolet spectrophotometer of Thermo company, measuring the content of tannins in the sample with a 1260Infinity high performance liquid chromatograph of Agilent company, and measuring the content of saponins with an X-treme L C ultra high pressure liquid chromatograph of Jasco company, wherein the specific contents are shown in Table 4 (mass percent):
TABLE 4 comparison of the effects of different extraction methods
| Sample (I) | Content of flavonoids | Content of tannins | Saponins content |
| a | 4.71 | 6.86 | 3.14 |
| g | 5.48 | 8.28 | 3.94 |
| l | 4.97 | 5.12 | 2.62 |
| m | 1.51 | 1.59 | 0.98 |
| n | 2.72 | 3.37 | 1.24 |
The results in table 4 show that the contents of flavone, tannin and saponins in the samples a, g and l extracted by the eutectic solvent are far higher than those in the samples m and n prepared by the traditional extraction method; when the mixture ratio of the eutectic solvent is 1:3 choline chloride and glycerol (group g), the content of the above 3 active substances is 2-4 times of that of the water decoction method and the ethanol reflux method. The extraction method of the eutectic solvent can extract the active ingredients in the traditional Chinese medicine to the maximum extent, and has high extraction efficiency.
The extraction method of the eutectic solvent does not need repeated extraction, and is simple to operate; the heating temperature is low, the extraction time is short, and the energy consumption is low; the solvent of the traditional water decoction method and the ethanol reflux method is used for one time, the solvent is added again during the second extraction, and the eutectic solvent can be repeatedly used and extracted for a plurality of times, so that the pollution to the environment is less.
Examples 3 to 6 eye creams containing the Chinese medicinal composition of the present invention
1. Weighing the traditional Chinese medicine composition with the total weight of 1 kg according to the weight parts shown in the table 5.
TABLE 5
| Components | Example 3 | Example 4 | Example 5 | Example 6 |
| |
19 | 25 | 25 | 25 |
| |
25 | 25 | 20 | 25 |
| Poria cocos (Schw.) wolf | 18 | 15 | 15 | 18 |
| White atractylodes rhizome | 12 | 15 | 18 | 15 |
| Radix astragali | 14 | 10 | 12 | 10 |
| Radix Gentianae | 12 | 10 | 10 | 7 |
2. The preparation of the traditional Chinese medicine composition comprises the following steps:
(1) the method comprises the steps of mixing choline chloride and glycerol according to a molar ratio of 1:3, and mixing and dissolving the obtained mixture and deionized water according to a mass ratio of 7:3 to prepare the eutectic solvent.
(2) Mixing the traditional Chinese medicines according to the parts by weight in the table 5, crushing, wetting and soaking by using the eutectic solvent prepared in the step (1), and standing in a closed state to prepare a liquid-solid mixture. The particle size of the powder, the time of the powder being left sealed, and the liquid-solid ratio of the solvent to the Chinese medicinal composition are shown in Table 6.
TABLE 6
| Example 3 | Example 4 | Example 5 | Example 6 | |
| Crushing size (mesh) | 40 | 60 | 80 | 60 |
| Closed standing time (hours) | 22 | 28 | 20 | 24 |
| Liquid-solid ratio (m L/g) | 12 | 14 | 17 | 15 |
| Extraction temperature (. degree.C.) | 50 | 55 | 65 | 60 |
| Ultrasonic power (W) | 200 | 250 | 300 | 350 |
| Microwave power (W) | 750 | 700 | 650 | 600 |
| Extraction time(min) | 120 | 80 | 40 | 20 |
(3) And (3) extracting the liquid-solid mixture prepared in the step (2) for a period of time under the heating condition with the assistance of ultrasound and microwave according to the conditions in the table 6 to prepare an extracting solution.
(4) Filtering the extracting solution prepared in the step (3) by using a 200-mesh filter screen, enriching and separating by using AB-8 type macroporous adsorption resin, eluting by using 80% butanediol, collecting eluent and filtering.
3. Preparation of eye cream containing Chinese medicinal composition
TABLE 7
The preparation method comprises the following steps:
(1) preparing raw materials according to the composition;
(2) weighing the phase A, sequentially adding the phase A into an emulsifying pot, heating to 85-90 ℃, homogenizing for 5 minutes, stirring, and keeping the temperature for half an hour;
(3) weighing the components of the phase B, sequentially adding the components into an oil pan, heating to 85-90 ℃, and uniformly stirring;
(4) slowly adding the material in the oil pan into an emulsifying pan under stirring, homogenizing for 5 minutes, vacuumizing, preserving heat, stirring for 20 minutes, and then starting cooling water;
(5) and (4) when the temperature of the mixture in the step (4) is reduced to 40 ℃, sequentially adding the components of the phase C and the phase D, vacuumizing and uniformly stirring, reducing the temperature to 38 ℃, and discharging.
Comparative examples 1 to 12 eye creams containing different Chinese medicinal compositions
1. Weighing the traditional Chinese medicine or the traditional Chinese medicine composition with the total weight of 1 kg according to the weight parts shown in the table 8.
TABLE 8
2. The traditional Chinese medicine composition is extracted and the eye cream containing the traditional Chinese medicine composition is prepared according to the preparation method of the embodiment 6.
Example 7 efficacy testing
The eye creams prepared in examples 3 to 6 and comparative examples 1 to 12 and commercially available eye creams (effective components are turmeric, rhodiola rosea, peony, bletilla striata, poria cocos, atractylodes macrocephala and ampelopsis japonica) were subjected to related tests:
the first efficacy test is as follows: ocular skin pigment value determination
The experimental population is as follows: the method comprises the steps of selecting 77 females with obvious dark circles on eyes, dividing the females into eleven groups with the age span of 29-60 years, and dividing each group into 7 females.
The experimental method comprises the following steps: five groups of subjects used the eye creams prepared in examples 3 to 6 and the commercially available eye creams, respectively, the eye creams containing the Chinese medicinal composition of the present invention and the eye cream containing no Chinese medicinal composition were applied to the left and right eyes, and the eye creams prepared in comparative examples 1 to 12 were applied to the other six groups. When the eye cream is used, the forefinger is dipped in the eye cream with the size of rice grains and is spotted below eyes, then circular massage is carried out for 5-6 circles along the inner canthus, lower eyelids, tail of eyes and upper eyelid, and finally the index finger is used for lightly tapping the skin of the eyes, particularly the lower eyelid part, until the eye cream is completely absorbed. The subject is applied once in the morning and evening every day for 28 days,
evaluation indexes are as follows: after 28 days, the ocular skin pigment values were tested using a Q2000 direct-reading skin pigment tester from Shanghai optical instruments, Inc. Because of the difference of the individual skin background colors, the comparison of the direct reading numerical values is not practical, the experiment takes the numerical value difference of the positions 15mm and 25mm directly below the center of the pupil as the comparison base number, the average value change of the numerical value difference of the days 0, 14 and 28 is recorded, and the result is shown in the table 9 and the figures 1-3.
TABLE 9
As can be seen from the graph in FIG. 1, the difference between the values of the subjects at 15mm directly below the center of the pupil and at 25mm directly below the center of the pupil at day 28 in the examples 3-6 is obviously reduced (and the change rate reaches 50 percent), which shows that the difference of the eye pigment of the eye using the eye cream in the examples 3-6 is reduced and the dark circles are obviously lightened. The comparative examples are almost unchanged, which shows that the traditional Chinese medicine composition has good black eye fading effect; meanwhile, compared with the commercially available eye cream under the same test condition, the black eye fading effect of the eye cream is obviously better than that of the commercially available eye cream (the change rate is only 28.90%).
As can be seen from FIG. 2, in example 6, compared with comparative examples 1 to 6, the change rate is significantly improved, and the change rate is more than 2 times of each comparative example, which shows that the black eye fading effect of the composition of six components is significantly better than that of any single traditional Chinese medicine.
As can be seen from FIG. 3, the change rate of example 6 is obviously improved compared with that of comparative examples 7-12, and the change rate is more than 1.5 times of that of each comparative example, so that the black eye fading effect of the composition containing six components is obviously better than that of the composition containing any five components in the comparative examples; meanwhile, the six components are mutually promoted, synergistic effect is realized, the optimal combination is realized, and the disadvantages of one component and the other component are not met.
And (5) efficacy test II: determination of microcirculation blood flow value of eye skin
Efficacy test one for the subjects of example 6, commercial eye cream, comparative example 7 and comparative example 8, the skin microcirculation blood perfusion values of the lower eyelid parts were measured by using a MOOR L D L S laser doppler line scanning imager of the company MOOR, u.k. the mean blood perfusion values of the 0 th day, 14 th day and 28 th day were recorded, and the change trend was observed.
As can be seen from FIG. 4, the blood flow change value of the eye cream group in example 6 is significantly higher than that of the commercially available eye cream group and that of the groups in comparative examples 7 and 8, which indicates that the traditional Chinese medicine composition provided by the invention can significantly increase the blood flow of the skin of the lower eyelid, has a good effect of improving skin microcirculation, and can improve various eye skin problems caused by eye microcirculation.
And (3) efficacy test: pouch edema removal effect test
The experimental population is as follows: the method comprises the steps of selecting 45 women with obvious eye bags and eye edema on eyes, randomly dividing the women into three groups with the age span of 35-55 years, and randomly dividing the women into 15 persons in each group.
The experimental method comprises the following steps: one group used the eye cream without the Chinese medicinal composition of the present invention and was referred to as a control group, one group used the eye cream of example 6, and one group used the eye cream of comparative example 7. When the eye cream is used, the forefinger is dipped in the eye cream with the size of rice grains and is spotted below eyes, then circular massage is carried out for 5-6 circles along the inner canthus, lower eyelids, tail of eyes and upper eyelid, and finally the index finger is used for lightly tapping the skin of the eyes, particularly the eye bags, until the eye cream is completely absorbed.
Evaluation indexes are as follows: the subjects were applied once a day, in the morning and at night, for 56 days, and observed eye edema and change of eye bags on day 0, day 28, and day 56 respectively in standard light mode of VISIA-CR facial image analyzer from Canfield, and the effect of edema after eye bag removal was objectively scored by professional testers: 0 points represent no effect, 1-3 points represent effect, 4-6 points represent obvious effect, and 7-9 points represent obvious effect. The results are shown in Table 11.
TABLE 11
The test shows that the results of the control example group and the comparative example group in 28 days and 56 days are mostly concentrated on no effect or slight effect, the results of the example 6 group in 28 days are mostly concentrated on effective effect or obvious effect, and the results of the example 6 group in 56 days are mostly concentrated on obvious effect or obvious effect, which shows that the example 6 has obvious effect of lightening the eye bags and the eye edema compared with the comparative example and the comparative example 7.
Claims (36)
1. The traditional Chinese medicine composition is characterized by comprising the following components in parts by weight: 15-35 parts of ligusticum wallichii, 13-32 parts of safflower, 9-24 parts of poria cocos, 8-20 parts of bighead atractylodes rhizome, 5-15 parts of astragalus membranaceus and 3-15 parts of gentian;
the preparation method of the traditional Chinese medicine composition comprises the following specific steps:
(1) crushing the traditional Chinese medicines according to the weight part ratio, soaking the crushed traditional Chinese medicines in a eutectic solvent, and sealing and placing the mixture to obtain a liquid-solid mixture;
(2) heating the liquid-solid mixture prepared in the step (1), and performing ultrasonic and microwave assisted extraction to prepare an extracting solution;
(3) filtering the extracting solution prepared in the step (2) by using a filter screen, enriching and separating by using adsorption resin, eluting, collecting eluent and filtering;
the eutectic solvent in the step (1) is a mixture of one of choline chloride or betaine and polyhydric alcohols.
2. The preparation method according to claim 1, wherein the particle size of the pulverized traditional Chinese medicine in the step (1) is 6-200 meshes.
3. The preparation method according to claim 1, wherein the particle size of the pulverized traditional Chinese medicine in the step (1) is 20-100 meshes.
4. The preparation method according to claim 1, wherein the particle size of the pulverized traditional Chinese medicine in the step (1) is 40-80 meshes.
5. The method according to claim 1, wherein the eutectic solvent in the step (1) is choline chloride and glycerol.
6. The preparation method according to claim 5, wherein the eutectic solvent choline chloride and glycerol in the step (1) are in a molar ratio of 1: 1-3.
7. The method according to claim 1, wherein the eutectic solvent has a water content of 5 to 50%.
8. The method according to claim 1, wherein the eutectic solvent has a water content of 10 to 45%.
9. The method according to claim 1, wherein the eutectic solvent has a water content of 25 to 35%.
10. The preparation method according to claim 1, wherein the liquid-solid ratio of the eutectic solvent to the traditional Chinese medicine mixture powder is 5-20 m L/g.
11. The preparation method according to claim 1, wherein the liquid-solid ratio of the eutectic solvent to the traditional Chinese medicine mixture powder is 12-17 m L/g.
12. The preparation method according to claim 1, wherein the closed standing time in the step (1) is 1-72 h.
13. The preparation method according to claim 1, wherein the closed standing time in the step (1) is 8-48 h.
14. The preparation method according to claim 1, wherein the closed standing time in the step (1) is 20-28 h.
15. The method according to claim 1, wherein the heating temperature in the step (2) is 40 to 70 ℃.
16. The production method according to claim 1, wherein the heating temperature in the step (2) is 60 ℃.
17. The preparation method according to claim 1, wherein the ultrasonic power in the step (2) is 200-400W.
18. The method according to claim 1, wherein the ultrasonic power in the step (2) is 350W.
19. The preparation method according to claim 1, wherein the microwave power in the step (2) is 500-750W.
20. The method according to claim 1, wherein the microwave power in the step (2) is 600W.
21. The method according to claim 1, wherein the extraction time in the step (2) is 5 to 120 min.
22. The method according to claim 1, wherein the extraction time in the step (2) is 20 min.
23. The preparation method according to claim 1, wherein the sieve in the step (3) is 80-300 mesh.
24. The method according to claim 1, wherein the sieve in the step (3) is 200 mesh.
25. The method according to claim 1, wherein the adsorbent resin in the step (3) is a macroporous adsorbent resin.
26. The method for preparing the macroporous resin of claim 25, wherein the macroporous resin in the step (3) is AB-8 type, X-5 type or D101 type.
27. The method as claimed in claim 25, wherein the macroporous adsorbent resin in step (3) is AB-8 type.
28. The method according to claim 1, wherein the eluent in the step (3) is any one or a combination of at least two of ethanol, water or butanediol.
29. The method according to claim 1, wherein the eluent in the step (3) is a combination of butanediol and water.
30. The method according to claim 29, wherein the mass fraction of butanediol in the eluent is 50 to 90%.
31. The method according to claim 29, wherein the mass fraction of butanediol in the eluent is 80%.
32. Use of the composition of claim 1 for the preparation of an eye care product for the reduction of dark circles under the eyes, bags under the eyes and edema.
33. An ophthalmic skin care product according to claim 32, comprising the following ingredients in weight percent:
the traditional Chinese medicine composition is prepared by the method of any one of claims 1 to 31, and the addition amount of the traditional Chinese medicine composition is 0.5-20%.
34. The eye skin care product according to claim 33, wherein the amount of the traditional Chinese medicine composition added is 3-10%.
35. The ocular skin care product of claim 33, wherein the amount of the traditional Chinese medicine composition added is 5%.
36. A method of making the ocular skin care product of claim 33, comprising the steps of:
(1) preparing raw materials according to the composition;
(2) weighing the phase A, sequentially adding the phase A into an emulsifying pot, heating to 85-90 ℃, homogenizing for 5 minutes, stirring, and keeping the temperature for half an hour;
(3) weighing the components of the phase B, sequentially adding the components into an oil pan, heating to 85-90 ℃, and uniformly stirring;
(4) slowly adding the material in the oil pan into an emulsifying pan under stirring, homogenizing for 5 minutes, vacuumizing, preserving heat, stirring for 20 minutes, and then starting cooling water;
(5) and (4) when the temperature of the mixture in the step (4) is reduced to 40 ℃, sequentially adding the components of the phase C and the phase D, vacuumizing and uniformly stirring, reducing the temperature to 38 ℃, and discharging.
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| CN113262179A (en) * | 2021-03-19 | 2021-08-17 | 深圳市萱嘉生物科技有限公司 | Plant extract composition with anti-allergy effect and preparation method and application thereof |
| CN112870131A (en) * | 2021-03-31 | 2021-06-01 | 深圳市萱嘉生物科技有限公司 | Traditional Chinese medicine extract composition with acne removing effect and preparation method thereof |
| CN113354610B (en) * | 2021-05-17 | 2023-04-25 | 浙江中医药大学 | Method for extracting flavonoid components in yin-nourishing qi-tonifying blood-activating prescription by natural eutectic solvent and process optimization method thereof |
| CN115006317A (en) * | 2022-07-27 | 2022-09-06 | 水羊化妆品制造有限公司 | Method for enriching fuding white tea flavone by using natural eutectic solvent and application |
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