A kind of Chinese medicine composition and preparation method
Technical field
The present invention relates to cosmetic fields, and in particular to a kind of Vitamin K, the Chinese medicine composition of eye pouch and oedema, system
Preparation Method and its application.
Background technique
Eye skin is the most thin position of human skin, and the thickness of epidermis and corium only has 0.25~0.55 millimeter, therefore
More easily influenced by external environment and body interior factor.Eye part skin lacks sebaceous glands and sweat gland is distributed, and can supply
Grease is opposite to want much less, the not natural moist ability of skin, so the skin than other positions is easier drying occur, lacks
The situation of few moisture.Sub-surface porosity connective tissue is abundant, is easy congested, ponding, and divides in the fibre structure of loose connective tissue
Cloth extremely fine and fragile capillary and lymphatic system, it is sensitive fragile, be highly prone to destroy and damage, cause local blood
Resistance, oedema.Seldom, with advancing age, collagen and elasticity are fine for collagen and elastomer distribution therein simultaneously
Dimension is lost, and periocualr skin becomes more loose and forms palpebra inferior edema, and Lymphatic Circulation and blood circulation become slower, blood, body
Liquid reflux is insufficient, and pigment, hydrops is caused to form black eye, oedema and eye pouch in connective tissue deposition.
Eye black eye is the emotional instability due to often staying up late, eye strain, aging, and vein blood vessel Hemodynamic environment is spent
In slow, red blood cell is for hypoxgia, and carbon dioxide and metabolic waste accumulation are excessive in vein blood vessel, forms chronic hypoxia,
Blood is darker and forms viscous flow and causes eye pigmentation.Age bigger people, the subcutaneous fat around eyes become to get over
It is thin, so black eye just becomes apparent from.
Ocular edema is that nighttime sleep is bad, eye part skin is impaired, and blood backflow goes wrong, and inflammation leads to capillary
Sepage occurs, is deposited in below eye.Chinese medicine is construed to:Stagnation of QI-blood, and cause retention of water-damp in the body, sends out as oedema, and gas does not run water
Wet, water-wet is poly-, and into swelling, the liquid of retardance is accumulated in eye part skin, forms ocular edema.
Eye pouch is that eye part skin aging is impaired, long-term microcirculation is bad, and retardance is obstructed to be caused to gather moisture and fat, eyelid
Around skin elasticity reduce, relaxation, orbicular muscle of eye relaxation, the fat of accumulation, hydrops are moved down in inferior orbit due to gravity
Form the satchel of protrusion.
Eye skin care item or effect object currently on the market are mostly from moisturizing, whitening, repair, diminish inflammation and react isogonism
Degree is set about to solve the problems, such as above-mentioned eye part skin for established presentation, and temporary solution is not effected a permanent cure.To black eye, eye pouch, eye water
Swollen to wait eye problems, the prior art is usually started with from Single-issue, targetedly improves a kind of symptom.
Eutectic solvent is a kind of novel green solvent of the substitution conventional solvent of recent hot spot, is by hydrogen bond receptor (chlorine
Change choline, glycine betaine etc.) it is combined with hydrogen bond donor (polyalcohol, carboxylic acids etc.) by hydrogen bond, freezing point is significantly lower than each group
Divide the solvent of fusing point.The advantages of eutectic solvent extracts is the water-soluble active ingredient that can not only extract in plant, such as more
Sugar, organic acid, can also extract the oil soluble active ingredients in plant, such as flavonoids, saponins, moreover it is possible to it is molten to extract water outlet oil
The all bad active constituent of solution property, such as tannins.Accordingly, it is considered to be used to the complexity of extraction efficiency and mentioned traditional Chinese medicinal components
Eutectic solvent is more advantageous to the extraction of effective component of chinese medicine.In addition, the raw material of eutectic solvent is very common compound,
Abundance and cheap, therefore, eutectic solvent extracting method are expected to realize industrialized production.
Summary of the invention
It is an object of the invention to propose a kind of Chinese medicine composition with Vitamin K, eye pouch and oedema.
The present invention also aims to propose a kind of extracting method of above-mentioned Chinese medicine composition active constituent.
The object of the invention is also to provide a kind of eye skin care item prepared using above-mentioned Chinese medicine composition.
The present invention starts with from the inherent origin cause of formation of eye problems, provide it is a kind of can Vitamin K, eye pouch and ocular edema
Eye-care Chinese medicine composition.
Chinese medicine composition of the invention is the monarch principle using drug matching, activating microcirculation and removing stasis medicinal, inducing diuresis for removing edema, tonifying Qi
Gu table, heat-clearing and damp-drying drug suit the medicine to the illness and solve to cause the inherent origin cause of formation of black eye, eye pouch and ocular edema multi-prongedly.
The composition of 1 the traditional chinese medicine composition of the invention of table and respective effect
The drug of composition prescription can be divided into monarch drug in a prescription by its effect played in prescription, ministerial drug, adjutant, make medicine, referred to as
Monarch minister, helps, makes.Monarch, which refers in prescription, plays the drug of primary treatment for main symptom, as required, can use simply or a few tastes.Minister
Refer to the drug of auxiliary monarch drug in a prescription treatment main symptom or primary treatment accompanying symptoms.Assistant assignment closes monarch-minister drug and treats accompanying symptoms, or inhibits monarch-minister drug
Toxicity, or play the drug of using corrigent.Make to guide the drug for leading the through diseased region of all medicines or coordinating the drug actions of a prescription.Yuan Dynasty Li Gao exists
《Treatise on the spleen and stomach》It writes:Monarch drug in a prescription component is most, and ministerial drug takes second place, and medicine is made to take second place again;Minister excessively monarch can not be enabled, the monarch and his subjects are orderly, Xiang Yuxuan
It takes the photograph, then can drive evil except disease.The main symptom that black eye, eye pouch and oedema are formed is stagnation of QI-blood, and accompanying symptoms is water-wet accumulation, therefore
Rhizoma Chuanxiong, safflower activating microcirculation and removing stasis medicinal are monarchs drug in a prescription in the present invention, and Poria cocos, Rhizoma Atractylodis Macrocephalae inducing diuresis for removing edema are ministerial drugs, and Radix Astragali invigorating qi for strengthening superficies strengthens the monarch and his subjects
Drug effect be adjutant, rough gentian coordinating the drug actions of a prescription is to make medicine.
Rhizoma Chuanxiong has blood-activating and qi-promoting, the effect of wind-expelling pain-stopping.Rhizoma Chuanxiong is the gas medicine in blood, nearly says its pungent scattered, relieve stagnation of residing, leads to
It reaches, has the function of improving microcirculation, expansion blood vessel.
Safflower has activating microcirculation and removing stasis medicinal, dissipate wet effect going to swell, and anticoagulant effect makes branch cyclic extention, increases blood flow, improves body
The ability of resist oxygen lack has apparent inhibiting effect to the penetrating amount increase of capillary of skin caused by histamine.
Poria cocos has the effect of eliminating dampness and diuresis, strengthening the spleen and stomach, antitoxic heart-soothing and sedative.Poria cocos can clearing damp and promoting diuresis and invigorating the spleen work
With the water-wet swelling for being unable to transporting for spleen deficiency has obvious effect, and dampness removing is without hurting healthy tendency.Relatively cold-dampness person, can be with ramulus cinnamomi, white
The compatibilities such as art, relatively damp and hot person can be with the compatibilities such as umbellate pore furgus, rhizoma alismatis;The person that belongs to Spleen-Qi Deficiency can match with Radix Codonopsis, astragalus, Rhizoma Atractylodis Macrocephalae etc.
5;Belong to cold of insufficiency type person, can also equally be used with monkshood, Rhizoma Atractylodis Macrocephalae.
Rhizoma Atractylodis Macrocephalae has strengthening the spleen and replenishing qi, and eliminate dampness and have diuretic effect is anti-oxidant, anticoagulant effect.
Radix Astragali has tonifying lung invigorating the spleen, invigorating qi for strengthening superficies, and effect of wind dispelling fortune poison is used for deficiency of vital energy and acking in strength, the sinking of qi of middle-jiao, and the deficiency of blood is withered
It is yellow.It is anti-oxidant for having in cosmetics, extend cell survival, promotes the active effect of SOD, lipid peroxide can be reduced to life
The damage of object film, delay skin aging.
Rough gentian, bitter is cold in nature, and root and rhizome is used as medicine.Primary efficacy is heat-clearing and damp-drying drug, and the effect of purging the liver of pathogenic fire gallbladder excess fire is very strong,
And it can relieve the wind syndrome analgesic.Regarding it as assistant makes, then all fire are all controlled.
To achieve the purpose of the present invention, technical scheme is as follows:
A kind of Chinese medicine composition includes following components in parts by weight:15~35 parts of Rhizoma Chuanxiong, 13~32 parts of safflower, Fu
9~24 parts of Siberian cocklebur, 8~20 parts of Rhizoma Atractylodis Macrocephalae, 5~15 parts of Radix Astragali, 3~15 parts of rough gentian.
A kind of preparation method of the Chinese medicine composition, specific step is as follows:
(1) it is immersed in eutectic solvent after crushing above-mentioned Chinese medicine by the weight, is obtained after closed placement
Liquid-solid mixture;
(2) liquid-solid mixture made from step (1) is heated, and combines ultrasound, microwave radiation exaraction, extracting solution is made;
(3) with absorption resin concentration separation, it will elute after extracting solution strainer filtering made from step (2), collect elution
Liquid filtering.
The granularity that the Chinese medicine of the step (1) crushes is 6~200 mesh;Preferably 20~100 mesh;Further preferably 40
~80 mesh.
Eutectic solvent in the step (1) is the mixing of choline chloride or glycine betaine alternative one and polyalcohols
Object;Preferably choline chloride and glycerol;It is furthermore preferred that the molar ratio of choline chloride and glycerol is 1: (1-3).
The water content of eutectic solvent in the step (1) is 5~50%;Preferably 10~45%;Further preferably
It is 25~35%.
The liquid-solid ratio of eutectic solvent and Traditional Chinese drug mixture powder in the step (1) is 5~20mL/g;Preferably 12
~17mL/g.
Closed standing time in the step (1) is 1~72h;Preferably 8~48h;Further preferably 20~28h.
Heating temperature in the step (2) is 40~70 DEG C;Preferably 60 DEG C.
Ultrasonic power in the step (2) is 200~400W;Preferably 350W.
500~750W of microwave power in the step (2);Preferably 600W.
Extraction time in the step (2) is 5~120min;Preferably 20min.
Strainer in the step (3) is 80~300 mesh;It is preferred that 200 mesh.
Absorption resin in the step (3) is macroporous absorbent resin;Preferably AB-8 type, X-5 type, D101 type;Into one
Step is preferably AB-8 type.
Eluent in the step (3) is the combination of any one or at least two in ethyl alcohol, water or butanediol;It is excellent
It is selected as the combination of butanediol and water;It is highly preferred that the mass fraction of butanediol is 50~90%;Most preferably 80%.
The Chinese medical extract that the method is prepared has effects that the eye of Vitamin K, eye pouch and oedema in preparation
Application in skin care item.
A kind of eye skin care item formula containing the Chinese medicine composition, includes following component by weight percentage:
The Chinese medicine composition is prepared according to the method, and additive amount is 0.5~20%;Preferably 3~
10%;Further preferably 5%.
A method of the eye skin care item being prepared, the method includes the following steps:
(1) prepare each raw material by the composition;
(2) A phase each component is weighed to be added sequentially to be heated to 85~90 DEG C in emulsion pot, homogeneous is stirred after five minutes,
Keep the temperature half an hour;
(3) it weighs B phase each component to be added sequentially to be heated to 85~90 DEG C in oil cauldron, stir evenly;
(4) Material in oil cauldron is slowly added in emulsion pot under stirring, homogeneous 5 minutes, vacuumizes heat preservation
Cooling water is opened in stirring after twenty minutes;
(5) when the mixture in the step (4) is cooled to 40 DEG C, C phase and D phase each component is sequentially added, vacuumizes and stirs
It mixes uniformly, is cooled to 38 DEG C, discharging.
The present invention has following beneficial effect:
(1) the traditional chinese medicine composition of the invention uses the monarch principle of drug matching, activating microcirculation and removing stasis medicinal, inducing diuresis for removing edema, tonifying Qi
Gu table, heat-clearing and damp-drying drug suit the medicine to the illness and solve to cause the inherent origin cause of formation of black eye, eye pouch and ocular edema, significant effect multi-prongedly;
(2) the traditional chinese medicine composition of the invention is mutually promoted using six kinds of Rhizoma Chuanxiong, safflower, Poria cocos, Rhizoma Atractylodis Macrocephalae, Radix Astragali and rough gentian components
Into, synergistic, the effect of playing significant Vitamin K, eye pouch and oedema, and it is safe and non-stimulating, it can be used as skin care
The raw material of product;
(3) present invention is using the effective elements of the medicine in eutectic solvent extraction, and extracting method is easy to operate, extraction efficiency
Height, it is time-consuming short, it is at low cost, it is conducive to industrialized production;Extracting eutectic solvent used simultaneously can recycle, and repeatedly extract,
High financial profit.
Detailed description of the invention
Fig. 1 embodiment 3~6 and commercially available eye cream and reference examples eye part skin pigment value difference change rate
1~6 eye part skin pigment value difference change rate of Fig. 2 embodiment 6 and comparative example
7~12 eye part skin pigment value difference change rate of Fig. 3 embodiment 6 and comparative example
Fig. 4 embodiment 6, commercially available eye cream and comparative example 7,8 palpebra inferior portion SkBF value changing values
Specific embodiment:
To make the purpose of the present invention, technical solution and advantage are clearer, below in conjunction with embodiment, comparative example to this hair
Bright do further is described in detail.Specific embodiment does not represent limiting the scope of the invention.Other people are according to the present invention
The some nonessential modifications and adjustment that theory is made still fall within protection scope of the present invention.
1 variety classes of embodiment and the eutectic solvent of proportion extract Chinese traditional medicine composition active substances
The Chinese medicine composition that total weight is 1 kilogram is weighed by parts by weight shown in table 2.
Table 2
Active Chinese drug component substance is extracted according to the following steps:
(1) by eutectic solvent mixture and deionized water with 7:3 quality prepares eutectic solvent than mixed dissolution.
(2) 40 mesh are ground into after mixing above-mentioned Chinese medicine each component by weight, the eutectic solvent prepared with step (1)
Wet to impregnate, closed to place 22 hours, the liquid-solid ratio of solvent usage and Chinese medicine composition weight is 12mL/g, and preparation solution mixes admittedly
Object.
(3) by the liquid-solid mixture of step (2) preparation in temperature 50 C, assisting ultrasonic power 200W, assisted microwave synthesis power
Under conditions of 750W, 120min is extracted, extracting solution is made;
(4) through AB-8 type macroporous adsorbing resin for purification point after extracting solution made from step (3) being filtered with 200 mesh filter screens
From with the elution of 80% butanediol, collection eluent filtering.
Only change the step the type and molar ratio of eutectic solvent in (1), the constant obtained Chinese medical extract of other conditions;Point
Not Yong Thermo company of the U.S. 220 ultraviolet specrophotometer of Evolution measurement extract in flavonoids content, with beauty
The content of tannins in the 1260Infinity high performance liquid chromatograph measurement extract of Agilent company of state, with Japanese Jasco
The content of saponins, the concrete content such as (quality hundred of table 3 in the X-treme LC ultrahigh-pressure liquid chromatograph measurement extract of company
Divide ratio):
The Chinese medicine composition activity substance content that 3 variety classes of table and the eutectic solvent of proportion extract
Group |
Solvent composition and molar ratio |
Flavone content |
Tannins content |
Saponine content |
a |
Glycine betaine/glycerol (1: 3) |
4.71 |
6.86 |
3.14 |
b |
Glycine betaine/glycerol (1: 1) |
4.58 |
6.65 |
3.01 |
c |
Glycine betaine/1,2 hexylene glycols (1: 3) |
4.82 |
5.75 |
3.36 |
d |
Glycine betaine/1,2 hexylene glycols (1: 1) |
4.15 |
5.28 |
3.53 |
e |
Glycine betaine/ethylene glycol (1: 3) |
3.22 |
5.38 |
2.81 |
f |
Glycine betaine/ethylene glycol (1: 1) |
3.57 |
5.67 |
2.92 |
g |
Choline chloride/glycerol (1: 3) |
5.48 |
8.28 |
3.94 |
g |
Choline chloride/glycerol (1: 1) |
4.56 |
6.37 |
2.84 |
i |
Choline chloride/1,2 hexylene glycols (1: 3) |
4.72 |
5.86 |
2.98 |
j |
Choline chloride/1,2 hexylene glycols (1: 1) |
4.17 |
5.31 |
3.16 |
k |
Choline chloride/ethylene glycol (1: 3) |
4.69 |
5.88 |
2.54 |
l |
Choline chloride/ethylene glycol (1: 1) |
4.97 |
5.12 |
2.62 |
As seen from the results in Table 3, it is made of by a certain percentage choline chloride or glycine betaine alternative one with polyalcohol low total
Molten solvent has good extractability to the active material of the traditional chinese medicine composition of the invention.When eutectic solvent is choline chloride and sweet
When oily (group g, h), the eutectic for being better than other types to the extraction effect of three kinds of active materials of the traditional chinese medicine composition of the invention is molten
Agent.When the molar ratio of choline chloride and glycerol in eutectic solvent is 1:(group g), the extraction to three kinds of active materials when 3
Effect is best.
The eutectic solvent extracting method of the present invention of embodiment 2 and traditional solvent extraction method Contrast on effect
The Traditional Chinese drug mixture that total weight is 1 kilogram is weighed by each Chinese medicine weight in embodiment 1, respectively with tradition
The Chinese traditional medicine composition active substances that water boiling method is extracted are sample m, the Chinese traditional medicine composition active substances extracted with ethanol refluxing process
As sample n.
Water boiling method the specific steps are:60 mesh are ground into after the Chinese medicine each component is mixed by weight, with distillation
Water-wet profit is impregnated, closed to place 48 hours, and the liquid-solid ratio of distillation water consumption and Chinese medicine composition weight is 20mL/g, and normal pressure decocts
90min is decocted 3 times, and decoction liquor room temperature is filtered after standing 2 hours with 200 mesh filter screens, by AB-8 type macroporous adsorbing resin for purification
Separation is eluted with 80% butanediol, collects eluent filtering;
Ethanol refluxing process the specific steps are:60 are ground into after the Chinese medicine composition each component is mixed by weight
Mesh, the ethanol water for being 80% with mass fraction is wet to be impregnated, closed to place 24 hours, solvent usage and Chinese medicine composition weight
The liquid-solid ratio of amount is 12mL/g, and atmospheric pressure reflux extracts 60min, and temperature is 70 DEG C, and extraction time is 2 times, and extracting solution room temperature stands 2
It is filtered after hour with 200 mesh filter screens, is separated by AB-8 type macroporous adsorbing resin for purification, eluted with 80% butanediol, collection is washed
De- liquid filtering.
Contained respectively with flavonoids in 220 ultraviolet specrophotometer of Evolution of Thermo company of U.S. measurement sample
Amount measures the content of tannins in sample with the 1260Infinity high performance liquid chromatograph of Agilent company of the U.S., with Japan
The content of the X-treme LC ultrahigh-pressure liquid chromatograph measurement saponins of Jasco company, concrete content such as (the quality percentage of table 4
Than):
4 Different Extraction Method Contrast on effect of table
Sample |
Flavone content |
Tannins content |
Saponine content |
a |
4.71 |
6.86 |
3.14 |
g |
5.48 |
8.28 |
3.94 |
l |
4.97 |
5.12 |
2.62 |
m |
1.51 |
1.59 |
0.98 |
n |
2.72 |
3.37 |
1.24 |
As seen from the results in Table 4, the flavones in the present invention in sample a, g, l of eutectic solvent extraction, tannin, saponins contain
Amount is all significantly larger than sample m, n made from traditional extraction process;It is 1 when eutectic solvent is proportion:3 choline chloride and glycerol
When (group g), the content of above-mentioned 3 kinds of active materials reaches 2-4 times of water boiling method and ethanol refluxing process.Eutectic of the invention
Solvent extraction process can extract the active constituent in Chinese medicine to the maximum extent, and extraction efficiency is high.
Eutectic solvent extracting method in the present invention does not need to extract repeatedly repeatedly, easy to operate;Heat required temperature
Low, extraction time is short, and energy charge is few;The solvent of traditional water decoction cooking method and ethanol refluxing process once utilizes, when extracting for the second time
Solvent is rejoined, and eutectic solvent can be recycled in the present invention, repeatedly extract, it is few for the pollution of environment.
Embodiment 3-6 contains the eye cream of the traditional chinese medicine composition of the invention
1. weighing the Chinese medicine composition that total weight is 1 kilogram by parts by weight shown in table 5.
Table 5
Component |
Embodiment 3 |
Embodiment 4 |
Embodiment 5 |
Embodiment 6 |
Rhizoma Chuanxiong |
19 |
25 |
25 |
25 |
Safflower |
25 |
25 |
20 |
25 |
Poria cocos |
18 |
15 |
15 |
18 |
Rhizoma Atractylodis Macrocephalae |
12 |
15 |
18 |
15 |
Radix Astragali |
14 |
10 |
12 |
10 |
Rough gentian |
12 |
10 |
10 |
7 |
2. the preparation of Chinese medicine composition:
(1) choline chloride and glycerol are pressed 1:3 molar ratio mixing, then by gained mixture and deionized water with 7:3
Quality prepares eutectic solvent than mixed dissolution.
(2) it being crushed after mixing the Chinese medicine by 5 parts by weight of table, the eutectic solvent prepared with step (1) is wet to be impregnated,
Closed placement, prepares liquid-solid mixture.Grinding particle size, closed standing time, solvent and Chinese medicine composition liquid-solid ratio parameter such as
Table 6.
Table 6
|
Embodiment 3 |
Embodiment 4 |
Embodiment 5 |
Embodiment 6 |
Grinding particle size (mesh) |
40 |
60 |
80 |
60 |
Closed standing time (hour) |
22 |
28 |
20 |
24 |
Liquid-solid ratio (mL/g) |
12 |
14 |
17 |
15 |
Extracting temperature (DEG C) |
50 |
55 |
65 |
60 |
Ultrasonic power (W) |
200 |
250 |
300 |
350 |
Microwave power (W) |
750 |
700 |
650 |
600 |
Extraction time (min) |
120 |
80 |
40 |
20 |
(3) liquid-solid mixture by step (2) preparation presses 6 condition of table in a heated condition, and assisting ultrasonic, microwave are extracted
For a period of time, extracting solution is made.
(4) through AB-8 type macroporous adsorbing resin for purification point after extracting solution made from step (3) being filtered with 200 mesh filter screens
From with the elution of 80% butanediol, collection eluent filtering.
3. the preparation of the eye cream containing Chinese medicine composition
Table 7
Preparation method:
(1) prepare each raw material by the composition;
(2) A phase each component is weighed to be added sequentially to be heated to 85~90 DEG C in emulsion pot, homogeneous is stirred after five minutes,
Keep the temperature half an hour;
(3) it weighs B phase each component to be added sequentially to be heated to 85~90 DEG C in oil cauldron, stir evenly;
(4) Material in oil cauldron is slowly added in emulsion pot under stirring, homogeneous 5 minutes, vacuumizes heat preservation
Cooling water is opened in stirring after twenty minutes;
(5) when the mixture in the step (4) is cooled to 40 DEG C, C phase and D phase each component is sequentially added, vacuumizes and stirs
It mixes uniformly, is cooled to 38 DEG C, discharging.
Comparative example 1-12 contains the eye cream of different Chinese medicine compositions
1. weighing the Chinese medicine or Chinese medicine composition that total weight is 1 kilogram by parts by weight shown in table 8.
Table 8
2. extracting the eye cream of Chinese medicine composition and preparation containing Chinese medicine composition according to the preparation method of above-described embodiment 6.
7 efficacy test of embodiment
By embodiment 3-6, (functional component is turmeric, root of kirilow rhodiola, Chinese herbaceous peony for eye cream made of comparative example 1-12 and commercially available eye cream
Medicine, the bletilla striata, Poria cocos, Rhizoma Atractylodis Macrocephalae, radix ampelopsis) carry out dependence test:
Efficacy test one:The measurement of eye part skin pigment value
Subject Population:Choosing eye has obvious black-eyed women, and totally 77 people, age range 29~60 years old, is divided into 11
Group, every group of 7 people.
Experimental method:Five groups of tested crowds use eye cream obtained by embodiment 3-6 and commercially available eye cream, right and left eyes point respectively
Not Tu Mo the eye cream containing the traditional chinese medicine composition of the invention and the eye cream control sample without Chinese prescription of the present invention, it is another six groups using pair
Eye cream obtained by ratio 1-12.The eye cream point of grain of rice size is dipped below eye with index finger when use, then along interior eye
Angle, palpebra inferior, eye tail, upper eyelid make the circle of circular massage 5~6, finally gently put bat eye part skin with index finger finger pulp, especially under
Eyelid position, until eye cream fully absorbs.Subject is sooner or later each daily to smear once, persistently uses 28 days,
Evaluation index:After 28 days, eye part skin is tested with Shanghai optical instrument factory Q2000 direct-reading type skin pigment tester
Pigment value.Since individual's skin background color has differences, merely using direct-reading numerical value as no practical significance is compared, this experiment is with pupil
Center is directly descended at 15mm and directly the lower place 25mm numerical difference is to compare radix, records the 0th day, and the 14th day, the 28th day numerical difference was put down
Change in Mean, as a result such as table 9 and Fig. 1-3.
Table 9
As seen in Figure 1, embodiment 3-6 is directly descended at 15mm and directly lower 25mm in the 28th Tian Shi subject pupil center
(and change rate reaches 50%) is obviously reduced in place's numerical difference, illustrates to reduce using the eye pigment difference of embodiment 3-6 eye cream,
Black eye is obviously desalinated.And each reference examples are almost unchanged, show that Chinese medicine composition of the invention has desalination black eye well
Enclose effect;It is compared under same test condition with commercially available eye cream simultaneously, the effect of Vitamin K is all substantially better than commercially available
Eye cream (change rate is only 28.90%).
As seen in Figure 2, compared with comparative example 1-6, change rate significantly improves embodiment 6, and change rate is each right
The 2 times or more of ratio illustrates that the effect of the composition Vitamin K of six kinds of components in the present invention is substantially better than in any single
Medicine.
As seen in Figure 3, compared with comparative example 7-12, change rate significantly improves embodiment 6, and change rate is each right
1.5 times or more of ratio illustrate that the effect of the composition Vitamin K of six kinds of components in the present invention is substantially better than in comparative example
The composition of any five kinds of components;Also illustrate that six kinds of components are mutually promoted simultaneously, synergistic, optimum combination is indispensable.
Efficacy test two:The measurement of eye part skin microcirculation blood flow value
6 groups of embodiment are directed in efficacy test one, commercially available eye cream group, 7 groups of comparative example, the subject that 8 groups of comparative example uses English
The MoorLDLS laser-Doppler line scanning imaging instrument of MOOR company of state tests the skin microcirculation blood perfusion in its palpebra inferior portion
Value records the 0th day respectively, and 14 days, 28 days blood perfusion amount average value observed variation tendency.These subjects are without mouth
Vasoactive agent history is taken, normal living habit is kept in subject's test period, tests bland diet in first 48 hours,
Forbid drinking, coffee and strong tea, avoids strenuous exercise.As a result such as table 10 and Fig. 4.
Table 10
As seen in Figure 4,6 eye cream group blood flow changing value of embodiment is apparently higher than commercially available eye cream group, comparative example 7,8
Group shows that Chinese medicine composition of the present invention can obviously increase palpebra inferior portion skin blood flow, and have improves skin well
The effect of microcirculation, can the eye part skin problem caused by a variety of microcirculations as eye improve.
Efficacy test three:Remove eye pouch oedema measure of merit
Subject Population:The women that eye has obvious eye pouch, ocular edema is chosen, totally 45 people, age range 35~55 years old, with
Machine is divided into three groups, every group of 15 people.
Experimental method:One group uses the eye cream without the traditional chinese medicine composition of the invention, referred to as reference examples group, and one group uses implementation
The eye cream of example 6, one group of eye cream using comparative example 7.The eye cream point of grain of rice size is dipped below eye with index finger when use, so
Circular massage 5~6 is done along inner eye corner, palpebra inferior, eye tail, upper eyelid afterwards to enclose, and is finally gently put with index finger finger pulp and is clapped eye skin
Skin, especially eye pouch position, until eye cream fully absorbs.
Evaluation index:Subject is sooner or later each daily to smear once, persistently uses 56 days, respectively at the 0th day, the 28th day, the
Under 56 days VISIA-CR face-image analyzer standard optical modes with Canfield company of the U.S. observe subject's ocular edema,
The situation of change of eye pouch, by professional test personnel to going eye pouch oedema effect to carry out objective marking:0 point indicates to no effect, 1~3
Dividing indicates effective, and 4~6 points indicate positive effect, and 7~9 points indicate significant positive effect.As a result such as table 11.
Table 11
By this test as can be seen that reference examples group, comparative example group 28 days, 56 days results focus mostly on to no effect or is having light
Minor effect fruit, 6 groups of 28 days results of embodiment focus mostly on effective or have positive effect, and 56 days results focus mostly on and having positive effect
Or have remarkable result, show that embodiment 6 compares reference examples and comparative example 7, there is the effect of apparent desalination eye pouch, ocular edema.