CN105998448A - Method for preparing mulberry oral solution for improving immunity and oral solution prepared by same - Google Patents

Abstract

The invention discloses a preparation method of mulberry oral liquid for improving immunity, which comprises the following steps: a. extracting mulberry as component 1; b. putting the remaining raw materials into ethanol and heating and refluxing to extract twice, As component 2; c. Combine the above two extract components 1 and 2, concentrate, add honey or dextrin to reconcile, disinfect and sterilize to prepare an oral liquid. In the step a, the mulberry fruits are harvested when they are ripe from April to June each year, washed, removed from impurities, dried in the sun or slightly steamed and then dried in the sun. The invention uses mulberry as the main ingredient, adds some traditional Chinese medicines that nourish the liver and kidney, promote blood circulation and nourish the marrow, and have mild nature and flavor, so as to obviously improve the taste. All medicines are used together to nourish lung gold, nourish kidney water, restrain lung qi, relieve cough and asthma, tonify kidney essence, and improve brain power, so it can improve intelligence and strengthen the body, and has a certain anti-aging effect.

Description

Preparation method of mulberry oral liquid for improving immunity and oral liquid thereof

technical field

The invention relates to medical health products containing raw materials derived from plants, animals or minerals, in particular to a mulberry oral liquid for improving immunity and a preparation method thereof.

Background technique

For millions of years, humans have lived in an environment that is both suitable for survival and fraught with danger. Human beings have survived and acquired extraordinary immunity. So immunity is a product of biological evolution.

Immunity is the body's own defense mechanism, which is the body's ability to recognize and eliminate any foreign matter (viruses, bacteria, etc.) . Modern immunology believes that immunity is the physiological response of the human body to recognize and eliminate "others". The body that performs this function is the immune system.

Immunity can be divided into: 1. Innate immunity is what a person is born with. For example, swine fever spreads quickly among pigs, but it has no relationship with humans. This is because humans are not born with this disease; 2. Acquired immunity is acquired naturally in the course of life after birth, or passively acquired with artificial assistance.

A body with a low immune system is prone to infection or cancer; an immune system with a high immune system can also produce harmful results, such as triggering allergic reactions, autoimmune diseases, etc.

For various reasons, the immune system cannot normally play a protective role. In this case, it is very easy to cause infections such as bacteria, viruses, and fungi. Therefore, the most direct manifestation of low immunity is that it is easy to get sick. Due to frequent illnesses, the consumption of the body has been aggravated, so there are generally symptoms such as physical weakness, malnutrition, listlessness, fatigue, loss of appetite, sleep disturbance, etc. Getting sick, taking injections and taking medicine have become commonplace. It takes a long time to recover from each illness, and often relapses. If things go on like this, it can lead to poor physical and mental development, and it is also easy to induce major diseases.

The underlying reason is low immunity or unsound immunity. When the human body's immune function is dysfunctional, or the immune system is not perfect, the following problems will recur: --recurrent colds, recurrent tonsillitis, recurrent asthma, recurrent bronchitis, recurrent pneumonia, recurrent diarrhea... ...so don't underestimate it.

Immunity refers to the ability of the body to resist external invasion and maintain the stability of the internal environment. The air is full of all kinds of microorganisms: bacteria, viruses, mycoplasma, chlamydia, fungi, etc. In the case of low human immunity, they can all become pathogens of the morning shift. Although the human body will produce corresponding antibodies against different pathogens to resist re-infection, antibodies are specific and time-limited. Can not resist other virus infection. People with low immunity cannot resist the invasion of cold viruses at all, which is the real reason for his frequent colds.

According to the research of biological scientists, the number of biological cell divisions is directly proportional to the life span. For example, the number of cell divisions of a dolphin is 80 times, and its average life span can reach 150 years. The reason for human aging is the decay of the most basic unit of human body life - cells. With the increase of age, the division of cell regeneration becomes slower and slower, and the accumulated aging cells make the functions of organs continue to decline, aging and even pathological changes occur, and people are also aging day by day.

Mulberry is the mature fruit of Moraceae deciduous tree mulberry tree. Mulberry is also called mulberry and mulberry jujube. Farmers like its ripe fresh fruit to eat. It has a sweet taste and a lot of juice. It is one of the fruits that people often eat. Traditional Chinese medicine believes that mulberry fruit nourishes the liver and kidney, nourishes yin and nourishes blood, and relieves wind. Mulberries can improve the blood supply of the skin (including the scalp), nourish the skin, make the skin white and tender and black hair, and can improve immunity. Mulberry is a good fruit and good medicine for middle-aged and elderly people's health, beauty and anti-aging. Eating mulberries regularly can improve eyesight and relieve the symptoms of eye fatigue and dryness. Mulberries have an immune-boosting effect. Mulberry has a weight-increasing effect on the spleen, enhances the hemolytic reaction, prevents human arteriosclerosis, bone and joint sclerosis, and promotes metabolism. It can promote the growth of red blood cells, prevent leukopenia, and has auxiliary effects on the treatment of diabetes, anemia, hypertension, hyperlipidemia, coronary heart disease, neurasthenia and other diseases. Mulberry has the effects of promoting body fluid to quench thirst, promoting digestion, and helping defecation. Eating in moderation can promote the secretion of gastric juice, stimulate intestinal peristalsis and relieve dryness and heat. Chinese medicine believes that mulberries are sweet and cold in nature and have the effects of nourishing the liver and kidney, promoting body fluid and moistening the intestines, and blackening the eyes.

More than 2,000 years ago, mulberry was a tonic used by Chinese emperors. Because of the special growth environment of the mulberry tree, the mulberry fruit has the characteristics of natural growth and no pollution, so the mulberry is also known as the "folk holy fruit". Modern research has confirmed that mulberry fruit is rich in active protein, vitamins, amino acids, carotene, minerals, resveratrol, anthocyanins and other ingredients, and its nutrition is 5-6 times that of apples and 4 times that of grapes. It has a variety of effects, and is praised as "the best health fruit in the 21st century" by the medical profession. Eating mulberry regularly can significantly improve human immunity, and has the effects of improving immunity and beautifying the skin.

Traditional Chinese medicine believes that mulberries are sour in taste and slightly cold in nature. Sore knees, premature graying of beard and hair, quenching thirst and quenching dryness, dry stool, nourishing yin and blood, soothing the nerves and nourishing the brain, sharpening joints, eliminating rheumatism, hangover and other effects.

1. Prevent arteriosclerosis: mulberry contains fatty acids, mainly linoleic acid. Composed of stearic acid and oleic acid, it has the functions of decomposing fat, reducing blood fat, and preventing hardening of blood vessels.

2. Strengthen the spleen and stomach and help digestion: mulberry contains tannic acid, fatty acid, malic acid and other nutrients, which can help the digestion of fat, protein and starch, so it has the function of strengthening the spleen and stomach and helping digestion, and can be used to treat indigestion caused by indigestion. diarrhea.

3. Supplementary nutrition: Mulberry contains a lot of water, carbohydrates, multivitamins, carotene and trace elements necessary for the human body, etc., which can effectively expand the blood volume of the human body, and it is not greasy. It is suitable for high blood pressure and gynecological diseases. patient diet.

4. Black hair beauty: Mulberry contains a lot of nutrients needed by the human body, and also contains black hair, which can make the hair black and shiny, and can be used for beauty.

Mature mulberries are rich in nutrients, containing 81.8 grams of water, 1.8 grams of protein and 0.3 grams of fat per 100 grams of mulberries. 4.9 grams of cellulose, 10 grams of carbohydrates, 1.2 grams of ash, 30 micrograms of carotene, 0.02 mg of thiamine, 0.06 mg of riboflavin, 6.95 mg of vitamin E, 33 mg of potassium, 0.27 mg of zinc, 0.08 mg of copper, selenium 4.8 micrograms. In addition, it also contains tannic acid, malic acid, vitamin C and fatty acids. Its fat is mainly linoleic acid, oleic acid, palmitic acid, stearic acid and a small amount of caprylic acid, nonanoic acid, capric acid, myristic acid, linolenic acid and so on.

Traditional Chinese medicine has a bitter smell and a bad taste; many people are unwilling to take it; people also feel resistance when using it. How to maximize the sweet, nutritious and anti-aging effects of mulberry without harming the human body, and obviously improve the taste so that people can accept it is a problem that we urgently need to solve. Mulberry contains a large number of safe and effective anti-aging and beauty ingredients; it is non-toxic and can be completely absorbed by organisms, which is the main direction of our research.

Contents of the invention

The technical problem to be solved by the present invention is that the traditional Chinese medicine smells bitter and has a bad taste; many people are unwilling to take it; people also feel resistance when using it. The immunity of modern people is becoming more and more fragile. Environmental pollution, irregular work and rest, fast-paced life, unhealthy diet, and lack of abstinence have caused the incidence of various diseases to continue to rise. Especially the so-called office workers are high-risk groups with low immunity. Most people are in a sub-healthy state, overdrawing their unhealthy bodies. Symptoms of most people are easy to catch a cold, sore eyes, spleen deficiency and weakness; lack of food, dry mouth; thirst; As the contemporary white-collar workers age, their kidney essence gradually becomes weak, their physical strength declines day by day, and their brain power decreases. Mulberry tonifying kidney essence improves brain power, so it can improve intelligence and strengthen the body. Experimental studies have shown that mulberry has certain anti-aging effects in improving immunity, nourishing the liver and kidney . The present invention uses mulberries as the main ingredient, and adds some traditional Chinese medicines that nourish the liver and kidney, strengthen tendons and bones, promote blood circulation and nourish marrow, and have mild nature and flavor. It has greatly improved and has a strong market prospect; the combination of various medicines can nourish the lungs, nourish the kidneys, restrain the lungs, relieve cough and asthma, nourish the kidney essence, and improve brain power, so it can improve the mind and strengthen the body, and has a certain anti-aging effect on the human body , is a health product that improves immunity.

In order to solve the above-mentioned technical problems, the present invention provides a mulberry oral liquid for improving immunity. The mulberry oral liquid may include the following raw materials in parts by weight: 10-50 parts of mulberry, 10-20 parts of curculigo, 10-20 parts of Rehmannia glutinosa 20 parts, Schisandra 10-20 parts, Dandelion 10-20 parts, Poria cocos 10-20 parts, Digupi 10-20 parts, Wujiapi 10-20 parts, Shouwu 10-20 parts, Cornus officinalis 10-20 parts , 10-20 parts of mulberry, 10-20 parts of Gynostemma, 10-20 parts of Astragalus, 10-20 parts of Weeping grass, 10-20 parts of Codonopsis, 10-20 parts of Ligustrum lucidum, 10-20 parts of dodder, Danpi 10-20 parts, gardenia 10-20 parts.

The mulberry oral liquid for improving immunity may also include the following raw materials in parts by weight: 10-40 parts of mulberry, 10-15 parts of curculigo, 10-15 parts of Rehmannia glutinosa, and 10-15 parts of schisandra , 10-15 parts of Dandelion, 10-15 parts of Poria, 10-15 parts of Digupi, 10-15 parts of Wujiapi, 10-15 parts of Shouwu, 10-15 parts of Cornus officinalis, 10-15 parts of Sangjishen, 10-20 parts of Gynostemma pentaphyllum, 10-20 parts of Astragalus membranaceus, 10-20 parts of weeping grass, 10-20 parts of Codonopsis pilosula, 10-20 parts of Ligustrum lucidum, 10-20 parts of dodder, 10-20 parts of paeonol, 10 parts of gardenia ~20 servings.

The mulberry oral liquid for improving immunity may also include the following raw materials in parts by weight: 10-30 parts of mulberry, 10-20 parts of curculigo, 10-20 parts of Rehmannia glutinosa, and 10-20 parts of Schisandra chinensis , 10-20 parts of Dandelion, 10-20 parts of Poria cocos, 10-20 parts of Digupi, 10-20 parts of Wujiapi, 10-20 parts of Radix Polygoni Multiflori, 10-20 parts of Cornus officinalis, 10-20 parts of Sangjisi, 10-15 parts of Gynostemma pentaphyllum, 10-15 parts of Astragalus membranaceus, 10-15 parts of Weeping grass, 10-15 parts of Codonopsis pilosula, 10-15 parts of Ligustrum lucidum, 10-15 parts of dodder, 10-15 parts of paeonol, 10 parts of gardenia ~15 servings.

In order to solve the above-mentioned technical problems, the present invention also provides a method for preparing the mulberry oral liquid for improving immunity, wherein the preparation steps of the oral liquid include:

a. the extraction of mulberry, as component 1;

b. put the rest of the raw materials into ethanol and heat and reflux to extract twice, as component 2;

c. Combining the above two extracts, component 1 and component 2, concentrating, adding honey or dextrin to reconcile, disinfecting and sterilizing to prepare an oral liquid.

In the step a, the mulberries can be harvested when the mulberry fruit is ripe in April to June every year, washed, removed from impurities, dried in the sun or slightly steamed and then dried in the sun.

In the step a, further comprising: soaking the air-dried mulberries in a stainless steel bucket with a cover, adding 70% ethanol solution, the mass of which is 0.8-1 times that of the coarse particles, stirring evenly, and placing them in a moist airtight place for more than 1 hour, so that Fully expand; wrap the filter plate at the bottom of the percolation cylinder with a gauze bag and spread it flat, then mix and loosen the wet and expanded drug samples, then use a stainless steel spoon to hold the powder, and evenly put it into the percolation cylinder, 10-12 cm Thick, use a T-shaped rod to press evenly, and then follow the above operation, load layer by layer, pressurize appropriately, the filling of the powder should not exceed 2/3 of the height of the percolation cylinder; the surface of the powder should be covered with a stainless steel orifice plate and pressed firmly , open the discharge valve under the percolation cylinder, put a container, and then slowly add 70% ethanol solution; after the air between the powder particles is discharged, and about 20L of ethanol flows out, close the discharge valve and cover Drum, impregnated for 24 hours, then open the material valve for percolation, control the percolation speed, generally 1000g of medicinal materials flow out 2-3ml per minute, put the filtrate into the storage tank, and pour the ethanol solution when emptying into the storage solution Cylinder; Combine the leachate and let it stand for later use as component 1.

In the step b, the rest of the raw materials can be put into ethanol, soaked for 1-2 days, then heated and extracted twice, each time for 1-2 hours, and the 2 extracts are combined and left standing; then the above ethanol The extracted dregs of raw medicinal materials are extracted twice with water and heated under reflux, each time for 1 to 2 hours, the two extracts are combined and left standing; the above two extracts are combined, concentrated under reduced pressure and the relative density is 1.35 at 80°C The filtrate was recovered with ethanol, and the specific gravity of the obtained filtrate was adjusted to 1.38, and the extract was obtained under reduced pressure, which became component 2.

In the step c, component 1 and component 2 can be combined, put into a double-effect vacuum concentrator, first concentrate the filtrate to a concentrated solution with a relative density of 1.38 at 80°C, and refrigerate at a low temperature of 0-5°C 24 hours; add 0.3% diatomite filter aid to the refrigerated liquid, filter, put the filtrate into a double-effect vacuum concentrator, concentrate to 0.1g extract per 1ml, add dextrin to reconcile, and prepare it after sterilization Oral solution.

In order to solve the above-mentioned technical problems, the present invention also provides a method for preparing an immunity-enhancing mulberry oral liquid, the preparation steps of which can also be:

a. The preparation of mulberries, as component 1;

b. chopping and extracting the rest of the raw materials as component 2;

c. Mix component 1 and component 2, concentrate and add appropriate amount of honey to blend, sterilize with ultraviolet light and bottle.

In the step a, soak the air-dried mulberries in ethanol for 1-2 days, heat and extract twice, each time for 1-2 hours, take the supernatant, combine the extracts, filter through 100-110 mesh, and then After ultrafiltration through an ultrafiltration column with a cut-off molecular weight of 6000-10000, the ultrafiltrate is concentrated under reduced pressure to an extract with a relative density of 1.36 at 80°C, heated and concentrated to a paste, and left to stand for later use to form component 1; the step b In the process, the rest of the raw materials can be mixed, chopped and passed through a 100-120 mesh sieve, and the fine powder is used after sieving, and the coarse particles are put into an acid and alkali-resistant dipping pot, and soaked with ethanol above 70 degrees at room temperature for 3-7 days , and then heat and reflux for extraction twice, each time for 1 to 2 hours, combine the two extracts and let stand, filter, and take the filtrate after separation; filter with gauze, add 50-60% ethanol to the residue, and continue at 60°C-70°C Extract for 2 hours, stir once every 10 minutes, filter with gauze, combine the extracts, concentrate into a paste to become component 2; in the step c, you can combine components 1 and 2, and put them into a double-effect vacuum concentration Concentrate to 80°C in a container with a relative density of 1.60. The concentrated ointment is blended with honey or dextrin, sterilized by ultraviolet rays, and bottled.

The present invention organically combines modern nutrition theory with traditional Chinese medicine health care theory. In view of the characteristics of dialectical treatment of traditional Chinese medicine and the advantage of less toxic and side effects of traditional Chinese medicine, the health care treatment of traditional Chinese medicine has attracted widespread attention. Mulberry fruit is sour in taste and slightly cold in nature. It has the functions of improving human immunity, nourishing blood and nourishing yin, promoting body fluid and quenching thirst, moistening intestines and eliminating dryness, etc. Early whitening, quenching thirst and quenching dryness, dry stool, nourishing yin and blood, calming the nerves and nourishing the brain, sharpening joints, eliminating rheumatism, hangover and other effects. Modern people's life is irregular and the body is gradually weak, physical strength is declining day by day, and brain power is declining. Mulberry tonifying kidney essence can improve brain power, so it can improve intelligence and strengthen the body. Experimental studies have shown that mulberry has certain anti-aging effects in improving immunity, nourishing liver and kidney. The invention uses mulberry as the main ingredient, adds some traditional Chinese medicines that nourish the liver and kidneys, and has a mild nature and flavor, so as to obviously improve the taste. The traditional Chinese medicines have a bitter smell, poor taste, and inconvenient use, making a great improvement and having a strong market prospect. ; Various medicines are used together to nourish lung gold, nourish kidney water, restrain lung qi, relieve cough and asthma, tonify kidney essence, and improve brain power, so it can improve intelligence and strengthen the body, and has a certain anti-aging effect on the human body. It is a health product that improves immunity.

detailed description

The health care effect of traditional Chinese medicine is very strong. If western medicine is likely to cause a large number of side effects in the body and cause adverse reactions in people, the effect of traditional Chinese medicine with less toxic side effects and long-term use is more obvious.

Human immunity is the defense function of the human body against external pathogenic microorganisms, or the defense system. Under normal circumstances, it can ensure that the human body is not invaded by external pathogenic microorganisms. Generally speaking, human immunity refers to the ability of the human body's immune system to resist the invasion of foreign pathogenic microorganisms. Under the condition of normal human immunity, the human body itself can resist the invasion of quite a few germs and avoid causing damage to the human body. Immunity plays an important role in the mechanism of human life, and the human body's nutrition, fatigue, mood, environment, disease, medication, radiation, etc. lead to the decline of its own immunity. More and more studies have shown that traditional Chinese medicine is playing an increasingly important role in the regulation of human immunity. The results of this experiment show that making tea of the present invention can improve the visceral index, phagocytosis ability of macrophages and the value-proliferating ability of T cells in mice with decreased immunity caused by cyclophosphamide. Immunity of mice is enhanced.

Traditional Chinese medicine believes that mulberry fruit nourishes the liver and kidney, nourishes yin and nourishes blood, and relieves wind. Mulberries can improve the blood supply of the skin (including the scalp), nourish the skin, make the skin white and tender and black hair, and can improve immunity. Mulberry is a good fruit and good medicine for middle-aged and elderly people's health, beauty and anti-aging. Eating mulberries regularly can improve eyesight and relieve the symptoms of eye fatigue and dryness. Mulberries have an immune-boosting effect. Mulberry has a weight-increasing effect on the spleen, enhances the hemolytic reaction, prevents human arteriosclerosis, bone and joint sclerosis, and promotes metabolism. It can promote the growth of red blood cells, prevent leukopenia, and has auxiliary effects on the treatment of diabetes, anemia, hypertension, hyperlipidemia, coronary heart disease, neurasthenia and other diseases. Mulberry has the effects of promoting body fluid to quench thirst, promoting digestion, and helping defecation. Eating in moderation can promote the secretion of gastric juice, stimulate intestinal peristalsis and relieve dryness and heat. Chinese medicine believes that mulberries are sweet and cold in nature and have the effects of nourishing the liver and kidney, promoting body fluid and moistening the intestines, and blackening the eyes.

More than 2,000 years ago, mulberry was a tonic used by Chinese emperors. Because of the special growth environment of the mulberry tree, the mulberry fruit has the characteristics of natural growth and no pollution, so the mulberry is also known as the "folk holy fruit". Modern research has confirmed that mulberry fruit is rich in active protein, vitamins, amino acids, carotene, minerals, resveratrol, anthocyanins and other ingredients, and its nutrition is 5-6 times that of apples and 4 times that of grapes. It has a variety of effects, and is praised as "the best health fruit in the 21st century" by the medical profession. Eating mulberry regularly can significantly improve human immunity, and has the effects of improving immunity and beautifying the skin.

Traditional Chinese medicine believes that mulberries are sour in taste and slightly cold in nature. Sore knees, premature graying of beard and hair, quenching thirst and quenching dryness, dry stool, nourishing yin and blood, soothing the nerves and nourishing the brain, sharpening joints, eliminating rheumatism, hangover and other effects.

1. Prevent arteriosclerosis: mulberry contains fatty acids, mainly linoleic acid. Composed of stearic acid and oleic acid, it has the functions of decomposing fat, reducing blood fat, and preventing hardening of blood vessels.

2. Strengthen the spleen and stomach and help digestion: mulberry contains tannic acid, fatty acid, malic acid and other nutrients, which can help the digestion of fat, protein and starch, so it has the function of strengthening the spleen and stomach and helping digestion, and can be used to treat indigestion caused by indigestion. diarrhea.

3. Supplementary nutrition: Mulberry contains a lot of water, carbohydrates, multivitamins, carotene and trace elements necessary for the human body, etc., which can effectively expand the blood volume of the human body, and it is not greasy. It is suitable for high blood pressure and gynecological diseases. patient diet.

4. Black hair beauty: Mulberry contains a lot of nutrients needed by the human body, and also contains black hair, which can make the hair black and shiny, and can be used for beauty.

Mature mulberries are rich in nutrients, containing 81.8 grams of water, 1.8 grams of protein and 0.3 grams of fat per 100 grams of mulberries. 4.9 grams of cellulose, 10 grams of carbohydrates, 1.2 grams of ash, 30 micrograms of carotene, 0.02 mg of thiamine, 0.06 mg of riboflavin, 6.95 mg of vitamin E, 33 mg of potassium, 0.27 mg of zinc, 0.08 mg of copper, selenium 4.8 micrograms. In addition, it also contains tannic acid, malic acid, vitamin C and fatty acids. Its fat is mainly linoleic acid, oleic acid, palmitic acid, stearic acid and a small amount of caprylic acid, nonanoic acid, capric acid, myristic acid, linolenic acid and so on.

Poria cocos is sweet, light, and flat in nature. When used as medicine, it has the functions of diuresis and dampness, spleen and stomach, and calming the mind. Modern medical research: Poria cocos can enhance the immune function of the body, and Poria cocos polysaccharide has obvious anti-tumor and liver-protecting effects. Poria cocos is sweet and flat in nature and flavor, and enters the heart, lung and spleen meridians. It has the effects of eliminating dampness and diuresis, invigorating the spleen and stomach, calming the mind and calming the nerves. It can cure dysuria, edema and fullness, phlegm retention and cough, vomiting, nausea, diarrhea, spermatorrhea, stranguria, palpitations, and amnesia. The diuresis of Poria cocos is achieved by invigorating the functions of the spleen and lungs, which is different from other traditional Chinese medicines that directly diuresis. For spleen deficiency diarrhea, taking Poria cocos can not only strengthen the spleen, but also ooze dampness. For diarrhea and leukorrhea caused by spleen deficiency, Poria cocos has the effect of taking care of both specimens and is often compatible with Codonopsis pilosula, Atractylodes macrocephala, and Chinese yam. It can be used as an adjuvant medicine for invigorating the lung and spleen and treating qi deficiency.

Cornus officinalis, mainly cures cold and heat in the heart, warms the middle, expels cold and warm arthralgia, removes three insects, and has the effects of strengthening yin and nourishing essence, calming the five internal organs, clearing the nine orifices, and stopping urination; long-term use can improve eyesight and strengthen the body long years. Cornus officinalis, also known as yam meat, medicinal jujube, real jujube, jujube skin, meat jujube, etc., is a commonly used and precious Chinese medicinal material in my country with a long history of application. It has been favored by ancient medicine for its special effects such as strengthening and calming, strengthening yang but not helping fire, nourishing yin but not greasy, astringent but not staying evil. Zhang Zhongjing created "Jin Kui Shen Qi Wan" with Cornus officinalis as the king. According to chemical analysis, Cornus officinalis contains active ingredients such as cornel, tartaric acid, gallic acid, malic acid, resin, tannins and multivitamins with strong physiological activities, which have pharmacological effects such as enhancing immunity, anti-inflammatory, and antibacterial. A common medicine in Chinese medicine.

Rehmannia glutinosa, also known as Rehmannia glutinosa or Fudi, belongs to the plant of Scrophulariaceae. It is a good Chinese herbal medicine. It has the effect of nourishing blood and nourishing yin. It is one of the main ingredients of Liuwei Dihuang Pills, an over-the-counter drug for deficiency syndromes, which is used for hot flashes, night sweats, nocturnal emission, and thirst due to deficiency of kidney yin. For all syndromes of liver and kidney yin deficiency, this quality moistens the kidney, nourishes kidney yin, replenishes essence and marrow, and is an essential medicine for invigorating kidney yin. The ancients called it "replenishing the five internal organs and true yin" and "replenishing true water". It is often used with yam and dogwood to treat liver and kidney yin deficiency, soreness of the waist and knees, nocturnal emission, night sweats, tinnitus, deafness and thirst, etc. It can nourish the liver and kidney, and benefit the essence, such as Liuwei Dihuang Wan

Trichosanthes trichosanthes is the root of Trichosanthes cucurbitaceae. It is a traditional Chinese medicine that clears away heat and purifies fire. Indications: Cure fever and thirst, quench one's thirst, jaundice, dryness of the lung and hemoptysis, carbuncle, hemorrhoids and flaccidity. For the treatment of diabetes, it is often used in conjunction with Yin-nourishing medicines to achieve the effect of treating both symptoms and root causes. "Compendium of Materia Medica": Trichosanthum, relieves stagnation and heat in the five internal organs, such as excessive heart fire and dry tongue and mouth, excessive lung fire and swollen throat and throat, excessive spleen fire and swollen mouth, tongue and teeth, excessive phlegm fire and restless cough. If the liver-fire is distended and drained, the bones of the kidney-fire are steaming and hot, or the carbuncle is ulcerated but the heat toxin persists, or the five jaundice, the body and the eyes are yellow, and the small water is dripping or astringent, it is all caused by the stagnation of fire and heat. Cause, only this agent can open stagnation, reduce phlegm fire, and can cure it. In addition, its nature is sweet and cold, and it is good at curing thirst. It can be used to cure deficiency and thirst from tonic, to cure fire thirst from cold medicine, to cure thirst from qi medicine, and to cure polydipsia from blood medicine, which is the key medicine for thirst.

Curculigo curculigo has the functions of invigorating kidney and yang, benefiting essence and blood, strengthening bones and muscles, promoting blood circulation and reducing swelling. Tonify kidney yang, strengthen muscles and bones, dispel cold and dampness. Treatment of impotence and cold essence, urinary incontinence, metrorrhagia, confidant cold pain, cold arthralgia of waist and feet, carbuncle, scrofula, yang deficiency and cold diarrhea. Impotence and essence-cold, wind-cold waist and knees, muscle and bone flaccidity and numbness embolism. Among the ancient prescriptions, there is Xianmao Pill, which can strengthen the muscles and bones, benefit the spirit, improve eyesight, and blacken the beard and hair. "Haiyao Materia Medica": Main wind, warming the waist and feet, calming the five internal organs, strengthening muscles and bones, and digesting food. Propagation and supplementation can treat the seven injuries of the husband, improve the eyes and ears, strengthen the tendons, fill the bone marrow, and benefit yang. "Rihuazi Materia Medica": cure all wind, tonify five labors and seven injuries, appetizing and lowering qi.

Digupi is cold in nature and sweet in taste. Functions and indications: cooling blood and removing steam, clearing lung and reducing fire. For yin deficiency hot flashes, bone steaming night sweats, lung heat cough, hemoptysis, epistaxis. Heat-clearing, cooling blood. Control fatigue, hot flashes and night sweats, cough and asthma due to lung heat, hematemesis, epistaxis, stranguria with blood, thirst, hypertension, carbuncle, malignant sore. "The Classic": "maintains the five internal evils, quenches one's thirst in the heat, and impedes the week." , beneficial to the large and small intestine, resistant to cold and heat."

Wujiapi is the dried root bark of the deciduous small shrub Wujia of Araliaceae. Also known as Nitraria, Eyebone, Chasing Windshi, Nanwujiapi, etc. Mainly produced in Hubei, Hunan, Henan, Anhui, Sichuan and other places. The traditional Chinese medicine Acanthopanax has the effects of dispelling wind-dampness, nourishing the liver and kidney, and strengthening bones and muscles. "Compendium of Materia Medica" records: "Replenishing the middle and nourishing Qi, treating rheumatism and impotence, strengthening the bones and muscles." Modern research shows that this product also has the functions of anti-tumor, anti-fatigue, reducing whole blood viscosity, and preventing the formation of atherosclerosis. Wujiapi is easy to use. It can be decocted, put into pills, powdered or soaked in Wujiapi wine, but it should be used with caution for those with yin deficiency and hyperactivity of fire.

Sangjisheng bitter; sweet; property flat. It has the effects of dispelling rheumatism, benefiting liver and kidney, strengthening muscles and bones, and preventing miscarriage. Enter the heart, kidney two warps. Function cures mainly: invigorating the liver and kidney; strengthening muscles and bones; dispelling rheumatism; preventing miscarriage. Mainly soreness of the waist and knees; flaccidity of muscles and bones; partial dryness of limbs; rheumatic arthralgia; headache and dizziness; restless fetal movement, metrorrhagia and bleeding. For rheumatic arthralgia, soreness of the waist and knees, weakness of muscles and bones, metrorrhagia, bleeding during pregnancy, fetal movement; hypertension.

Paeoniae Alba is cool in nature, bitter and sour in taste, slightly cold, has the functions of nourishing blood and softening the liver, calming the liver and relieving pain. It has the effects of constricting yin and sweating, and is suitable for symptoms such as yin deficiency and fever, irregular menstruation, pain in the chest, abdomen and hypochondriac, limb cramps, diarrhea and abdominal pain, spontaneous sweating, metrorrhagia, and leukorrhea. Paeoniflorin has the effects of antibacterial, antipyretic, anti-inflammatory, increasing coronary artery flow, improving myocardial nutritional blood flow, dilating blood vessels, resisting acute myocardial ischemia, inhibiting platelet aggregation, sedative, analgesic, antispasmodic, anti-ulcer, and regulating blood sugar. . White peony decoction can inhibit Shigella, Streptococcus pneumoniae, Escherichia coli, Salmonella typhi, Hemolytic streptococcus, Pseudomonas aeruginosa and so on. Blood deficiency and yin deficiency, pain in the chest and abdomen, flank pain, liver pain, cholecystitis, gallstone pain, diarrhea and abdominal pain, women's menstrual pain, spontaneous sweating, night sweats, gastrocnemius muscle spasm, spasm and pain in the limbs, restless legs syndrome and other diseases Suitable for consumption.

Radix Polygoni Multiflori is made by boiling raw Radix Polygoni Multiflori with black beans and then drying it in the sun. It is mainly used to treat symptoms such as weakness of the waist and knees, dizziness, palpitations, insomnia, and premature graying of hair caused by deficiency of liver and kidney, and loss of essence and blood. The black hair function of Polygonum multiflorum is exerted through the functions of nourishing the kidney, benefiting the essence, nourishing the blood and calming the mind. Shouwu has the functions of nourishing essence and blood, nourishing the liver and calming the nerves, strengthening the bones and muscles, and strengthening the kidneys. It is used for dizziness and dizziness due to blood deficiency, palpitation, insomnia, forgetfulness, chlorosis and fatigue, dizziness and tinnitus due to liver and kidney essence and blood deficiency, soreness of the waist and knees, Nocturnal emission collapse belt, premature graying of beard and hair embolism. angelica

Danshen bitter, slightly cold. GUIXIN, Liver Channel. Dispelling blood stasis and relieving pain, promoting blood circulation to stimulate menstrual flow, clearing away heart-fire and relieving restlessness. For irregular menstruation, amenorrhea dysmenorrhea, accumulation of lumps in the abdomen, tingling pain in the chest and abdomen, heat arthralgia pain, sore swelling and pain, vexation and insomnia. It is used for sternocostal and hypochondriac pain, rheumatic arthralgia, lumps in the abdomen, swelling and pain of sores, pain from falls, irregular menstruation, amenorrhea dysmenorrhea, postpartum stasis and pain, etc. It is effective in treating sternocostal pain, lumps in the abdomen, irregular menstruation and amenorrhea. "Compendium" contains: Danshen, according to "Furen Ming Theory", Siwu Decoction can be used to treat women's diseases, regardless of prenatal and postpartum, and the amount of menstrual fluid, but Danshen powder is the same, and the indications are the same. Salvia miltiorrhiza can break old blood, replenish new blood, give birth to a fetus, stop stillbirth, stop stagnation in metrorrhagia, and regulate meridians. Tanshinone, the main component of Danshen, has the effects of dilating blood vessels, improving circulation, and relieving intravascular coagulation. It has the effect of promoting blood circulation and promoting blood circulation in the liver, which is beneficial to liver cell regeneration.

Astragalus has the effects of invigorating qi and solidifying the surface, diuresis and swelling, detoxification and pus drainage, and muscle regeneration. The medicinal use of Astragalus has a history of more than 2,000 years. Modern research shows that Astragalus contains saponins, sucrose, polysaccharides, various amino acids, folic acid, selenium, zinc, copper and other trace elements. It can enhance the immune function of the body, protect the liver, diuretic, anti-aging, anti-stress, lower blood pressure and have a wide range of antibacterial effects. However, there are evidences such as exuberance of excess pathogens, stagnation of qi and dampness, stagnation of food accumulation, early onset of carbuncle or ulceration of heat and toxin. Radix Astragali has the effects of invigorating qi and strengthening the exterior, diuretic and detoxification, evacuating pus, astringing sores and promoting granulation. For qi deficiency and fatigue, lack of appetite, loose stools, subsidence of central qi, chronic diarrhea and prolapse of the anus, blood in the stool and metrorrhagia, spontaneous perspiration due to superficial deficiency, carbuncle and gangrene that are difficult to ulcerate, chronic ulceration without restraint, blood deficiency and chlorosis, internal heat and quenching thirst. "The Classic" records: "mainly carbuncle and gangrene, long-term sores, draining pus and relieving pain. Tonifies deficiency, and treats all kinds of diseases in children." "Nihuazi Materia Medica" records: "Helps Qi and strengthens muscles and bones, grows flesh and nourishes blood."

Ligustrum lucidum, sweet and bitter in taste, cool in nature, returns to liver and kidney meridian, has the merit of nourishing liver and kidney, benefiting yin and nourishing blood. Modern medical research has proved that Ligustrum lucidum contains ursolic acid, mannitol, glucose, fatty oil, etc., which have the effects of strengthening the heart, diuresis and protecting the liver. Bacteria and Escherichia coli have inhibitory effect. Ligustrum lucidum contains oleanolic acid, ligustolic acid, privetin, ursolic acid, stearic acid, oleic acid, mannitol, betulin, phospholipids and sugars such as privet fruit polysaccharide, arabinose, glucose etc., also contains 15 kinds of amino acids, 11 kinds of trace elements and volatile oil. Studies have shown that Ligustrum lucidum has hypoglycemic and blood lipid-lowering effects and anti-atherosclerosis, as well as antibacterial and antiviral effects. and Escherichia coli were inhibited. It has a moderate inhibitory effect on type B adenovirus. Elevate white blood cells: Ligustrum lucidum and its component preparations have certain effects on blood cells, and it can increase the decrease in white blood cells caused by chemotherapy. The compound containing Ligustrum lucidum also has the effect of increasing white blood cells. Antithrombotic effect: Oleanolic acid can accelerate the fluidity of platelet cells, weaken the collision between platelets, make them less likely to adhere and aggregate, and less likely to deposit on the intima of blood vessels, slow down or prevent thrombosis, and reduce lipids. The deposition of intima provides some experimental basis for the prevention and treatment of thrombotic diseases in the elderly. Anti-aging and Improving Immunity: Ligustrum lucidum decoction can increase the weight of immune organs thymus and spleen in mice; significantly increase the activity of serum hemolysin antibody in mice, and has a two-way regulating effect on the production of interleukin-2 by spleen lymphocytes in mice. Ligustrum lucidum has a significant immune enhancement effect, scavenging free radicals and anti-aging. Ligustrum lucidum prevention and treatment: cardiovascular disease, coronary heart disease, hyperlipidemia, diabetes, thrombocytopenia, leukopenia, chronic nephritis, chronic bronchitis, intractable insomnia, chronic benzene poisoning, tumor, malnutrition, immune function hypochondriac, seborrheic dermatitis

Schisandra chinensis is rich in organic acids, vitamins, flavonoids, phytosterols and lignans (such as schisandrin A, schisandrin B or schisandrin) with strong restoration effects. One of the few herbal medicines with great benefits, it can nourish qi and strengthen the liver, improve the efficiency of cells to eliminate waste, supply more oxygen, create and use energy, improve memory and sexual endurance. In ancient times, Russian hunters must take Schisandra chinensis to strengthen their body and invigorate their energy before hunting. Schisandra has extraordinary effects, including: 1. Liver protection and regeneration of liver tissue. The liver is the central detoxification organ of the human body, responsible for filtering out toxic substances so as not to cause harm to the human body. Schisandra has anti-inflammatory effects, Schisandra has anti-inflammatory effects to stop liver damage, activate anabolic processes to promote the repair of damaged liver cells, and can enhance the activity of deoxyribonucleic acid (DNA) synthesis and ornithine decarboxylase to regenerate the liver cell. Carbon tetrachloride (CCl4) is one of the most toxic substances to the liver. Numerous studies have shown that the potent hepatoprotective effect of Schisandra chinensis can counteract the toxic effects of CCl4. It can be seen that schisandra has great efficacy in protecting the liver from daily toxins.

Dandelion is sweet in nature and flavor, slightly bitter, cold. Return liver, stomach warp. Diuretic, laxative, anti-jaundice, choleretic and other effects. Treatment of heat, bloated, sores, internal hemorrhoids, red eyes and swelling, damp heat, jaundice, urination, phlegm, astringent pain, hemorrhoids, phlegm, phlegm, toothache, red eyes, sore throat, lung abscess, Intestinal abscess, damp-heat jaundice, hot dripping pain. Treatment of acute mastitis, lymphadenitis, scrofula, furunculosis, acute conjunctivitis, cold and fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection, etc. Dandelion can be eaten raw, stir-fried, and made into soup. It is a plant with both medicine and food.

Chuipencao, the name of traditional Chinese medicine. It is the dry whole herb of Sedum chinensis. Sweet, light, cool. Promoting dampness and reducing jaundice, clearing heat and detoxifying. It is often used for damp-heat jaundice, dysuria, carbuncle and sore. Return liver, gallbladder, small intestine channel. Efficacy dampness and jaundice, heat and detoxification. Cure mainly for damp-heat jaundice, dysuria, carbuncle sores.

The main active ingredients of Jiaogulan are Jiaogulan saponins, Jiaogulan glucosides (polysaccharides), water-soluble amino acids, flavonoids, multivitamins, trace elements, minerals, etc. Medicinal base source: It is the whole herb of Cucurbitaceae Gynostemma pentaphyllum. Nature and flavor: bitter; slightly sweet; cool in nature. Return through: return lung; Spleen; Kidney channel. Efficacy: Tonifying Qi and invigorating the spleen, resolving phlegm and relieving cough, clearing heat and detoxifying. Efficacy classification: tonic medicine; phlegm-resolving medicine; heat-clearing medicine. Indications: physical weakness, fatigue, loss of sperm due to fatigue, leukopenia, hyperlipidemia, viral hepatitis, chronic gastroenteritis, and chronic bronchitis.

Codonopsis Codonopsis is a traditional tonic medicine commonly used in China. In ancient times, Codonopsis Codonopsis produced in Shangdang area of Shanxi was the top grade. It has the effects of nourishing the middle and Qi, strengthening the spleen and lungs. Codonopsis pilosula has the effects of enhancing immunity, dilating blood vessels, lowering blood pressure, improving microcirculation, and enhancing hematopoietic function. In addition, it can improve the leukopenia caused by chemotherapy and radiotherapy.

Cuscuta Chinese medicine Cuscuta is the seed of the dicotyledonous plant medicine Convolvulaceae, Cuscuta, Southern Dodder, Golden Dengteng, etc. It can nourish the kidney and essence, nourish the liver and improve eyesight, strengthen the fetus and stop diarrhea. Nourishing liver and kidney, solidifying essence and reducing urination, preventing miscarriage, improving eyesight, and stopping diarrhea. For impotence and nocturnal emission, residual urination, enuresis, frequent urination, soreness of the waist and knees, dizziness and tinnitus, kidney deficiency and fetal leakage, restless fetal movement, spleen and kidney fetal leakage, restless fetal movement, diarrhea due to spleen and kidney deficiency; external treatment of vitiligo. Sieve the original medicine to remove mud, pick out impurities, and dry

Gardenia is bitter and cold, can divide the blood and clear away pathogenic heat, widen the intestines and laxative, and prevent cancer. Gardenia contains cellulose, which can prevent the occurrence of hemorrhoids and rectal cancer. Gardenia roots, leaves, and fruits can be used as medicine. It has the effects of purging fire and relieving restlessness, reducing inflammation and heat, clearing heat and diuresis, and detoxifying for external use. Gardenia, fruit, leaves and roots can be used as medicine, usually made of tea or decocted. Gardeniae contains geniposide, deshydroxygeniposide, gallinacein methyl ester and a small amount of shangenin. blood pressure etc. Among them, gardenia fruit is used as medicine to treat high fever, restlessness and insomnia, toothache due to excessive fire, aphtha of the mouth and tongue, nasal congestion, hematemesis, conjunctivitis, sore swelling, jaundice infectious hepatitis, favism, hematuria; external use to treat trauma Bleeding, sprains. The root is used as medicine to treat mainly infectious hepatitis, bruises, wind-fire toothache. "Compendium of Materia Medica" calls it "pleasant color, and it is used in "Qianjinyi" face cream." "Southern Yunnan Materia Medica" says it "reduces lung fire, relieves cough due to lung heat, stops epistaxis, and eliminates phlegm." : cold in nature, sweet and bitter in taste; enter lung, liver meridian. Efficacy and indications: Qingfei cough, topical hemostasis. Indications of lung heat cough, nose, expectoration, swelling and other diseases. Main ingredients: Gardenia contains triterpenes, gardenia acid A, B and linic acid. Contains volatile oils, including benzyl acetate and linalyl acetate, as well as pigment glycosides and wood honey. In addition, it also contains carbohydrates, protein, crude fiber and multivitamins.

Specific embodiments of the invention

Specific embodiment 1:

Harvest the mulberry fruit when it is ripe from April to June every year, wash it, remove impurities, and dry it in the sun or steam it slightly.

Soak the sun-dried mulberry into 10 times the amount of ethanol, soak for more than 2-5 days, heat and extract twice, and form component 1.

Curculigo curculigo, Rehmannia glutinosa, Schisandra chinensis, Dandelion, Poria cocos, Digupi, Wujiapi, Shouwu, Cornus officinalis, Sangjisi, Gynostemma pentaphyllum, Astragalus, Weeping grass, Codonopsis pilosula, Ligustrum lucidum, Cuscuta, Danpi , gardenia, put into 10 times the weight of ethanol, soak for more than 2-5 days, heat and extract twice, and become component 2.

Combine components 1 and 2 and place them in a double-effect vacuum concentrator. The concentrated ointment is blended with honey, sterilized by ultraviolet rays, and bottled.

Specific embodiment 2: harvest when the mulberry fruit is ripe in April-June every year, wash, remove impurities, dry in the sun or dry after slightly steaming. About 5000g. Soak 5000g of air-dried mulberries in a covered stainless steel bucket, add 70% ethanol solution, the mass is 0.8-1 times that of coarse particles, stir evenly, and keep it moist and airtight for more than 1 hour to fully expand; Wrap the filter plate with a gauze bag and lay it flat, then loosen the wet and swelled drug sample, then use a stainless steel spoon to fill the powder, evenly put it into the percolation cylinder, fill it with a thickness of 10-12 cm, and press it evenly with a T-shaped stick , and then according to the above operation, load layer by layer, pressurize appropriately, the powder filling should not exceed 2/3 of the height of the percolation cylinder; and put a container, then slowly add 70% ethanol solution; after the air between the powder particles is discharged, and about 20L of ethanol flows out, close the discharge valve, cover the drum, and soak for 24 hours, then Open the material valve for percolation, control the percolation speed, generally 1000g of medicinal materials flow out 2-3ml per minute, put the filtrate into the storage tank, and pour the ethanol solution when emptying into the storage tank; combine the leachate and let it stand , stand for later use as component 1. Curculigo 1200g, Rehmannia glutinosa 1300g, Schisandra 1300g, Dandelion 1200g, Digupi 1200g, Shouwu 1200g, Cornus officinalis 1200g, Sangji 1200g, Jiaogulan 1200g, Astragalus 1200g, Weeping grass 1200g, Codonopsis 1200g, Ligustrum lucidum 1200g, Cuscuta 1200g, paeonol 1200g, gardenia 1200g, put into 10 times the weight of ethanol, soak for 15 days, then heat and extract twice, each time for 1-2 hours, combine the extracts, take the supernatant after standing, and then Put it into a vacuum concentration tank, recover ethanol under reduced pressure, concentrate to a concentration of 0.6 g of crude drug/mL, and filter with suction until the relative density of the filtrate is 1.06 at 20°C, which becomes component 2. After combining components 1 and 2, put them into a double-effect vacuum concentrator, first concentrate the filtrate to a concentrated solution with a relative density of 1.38 at 80°C, and refrigerate at a low temperature of 0-5°C for 24 hours; add the refrigerated solution to 0.3% filter aid diatomite, filter, put the filtrate into a double-effect vacuum concentrator, concentrate to contain 0.1g extract per 1ml, add dextrin to reconcile, prepare oral liquid after sterilization.

Specific embodiment 3: harvest when the mulberry fruit is ripe in April-June every year, wash, remove impurities, dry in the sun or dry after slightly steaming. Soak 4000g of air-dried mulberries in 10 times the amount of ethanol, soak for 15 days, heat and extract twice, 1-2 hours each time, take the supernatant, combine the extracts, filter with 100-110 mesh, and then pass through the cut-off molecular weight It is ultrafiltered with an ultrafiltration column of 6000-10000, and the ultrafiltrate is concentrated under reduced pressure to an extract with a relative density of 1.36 at 80°C, heated and concentrated to a paste, and left to stand for later use to form component 1. Curculigo 110g, Rehmannia glutinosa 110g, Schisandra 110g, Dandelion 1200g, Digupi 1200g, Shouwu 1200g, Cornus officinalis 1200g, Sangji 1200g, Jiaogulan 1200g, Astragalus 110g, Weeping grass 110g, Codonopsis 1200g, Ligustrum lucidum 1200g, Cuscuta 110g, paeonol 1200g, gardenia 1200g, mix the rest of the raw medicinal materials, chop and pass through a 100-120 mesh sieve, sieve the fine powder for later use, and put the coarse particles into the acid and alkali resistant dipping pot, at room temperature, with 70 degrees Immerse the above ethanol together for 2 to 4 days, then heat and reflux to extract twice, each time for 1 to 2 hours, combine the two extracts and let stand, filter, separate and take the filtrate; filter with gauze, add 50-60% to the residue Continue leaching with ethanol at 60°C-70°C for 2 hours, stir once every 10 minutes, filter through gauze, combine the extracts, concentrate into a paste and become component 2. Combine components 1 and 2 and place them in a double-effect vacuum concentrator, concentrate to a concentrated solution with a relative density of 1.50 at 80°C, and refrigerate at a low temperature of 0-5°C for 24 hours; Filter with diatomaceous earth, put the filtrate into a double-effect vacuum concentrator, and concentrate to 0.1 per 1ml; the concentrated ointment is blended with dextrin, sterilized by ultraviolet rays, and bottled.

Specific embodiment 4: harvest when the mulberry fruit is ripe in April-June every year, wash, remove impurities, dry in the sun or dry after slightly steaming. About 3500g. Soak 3500g of sun-dried mulberries in 10 times the amount of ethanol for 2 days, heat and extract twice, 1-2 hours each time, take the supernatant, combine the extracts, filter with 100-110 mesh, and then pass the cut-off molecular weight to 6000 -10000 ultrafiltration column ultrafiltration, the ultrafiltrate is concentrated under reduced pressure to an extract with a relative density of 1.36 at 80°C, heated and concentrated to a paste, and left to stand for later use to form component 1. Curculigo 110g, Rehmannia glutinosa 110g, Schisandra 110g, Dandelion 1200g, Digupi 1200g, Shouwu 1200g, Cornus officinalis 1200g, Sangjisi 1200g, Jiaogulan 1200g, Astragalus 110g, Weeping grass 110g, Codonopsis 1200g, Ligustrum lucidum 1200g , Cuscuta 110g, paeonol 1200g, gardenia 1200g, put the crude drug into a pulverizer and pulverize it, put the granules into ethanol and heat and reflux to extract 2 times, each time for 1 to 2 hours, and combine the 2 extracts Put the medicinal dregs extracted by ethanol with 10 times the amount of water and heat and reflux to extract twice, each time for 1 to 2 hours, combine the two extracts and let stand, combine the above two extracts, as component 2; Combine components 1 and 2 under reduced pressure and concentrate the filtrate with a relative density of 1.50 at 80°C, recover ethanol, add dextrin or honey, sterilize with ultraviolet rays, and bottle.

Pharmacological toxicity test

Experimental example 1: acute toxicity test of the present invention

1. Test materials: Animals: Kunming mice, weighing 19-26g, half male and half male, bred by the biological laboratory of Shandong University. Medicine: the present invention (all raw materials are mixed and decocted twice, combined and filtered, and the medicinal solution is taken) contains 0.0365 mg/ml.

2. Method:

1. Calculation of LD50: adopt the modified Cole's method, mice are randomly divided into 5 groups, 10 in every group, half male and half male, the present invention is dissolved in distilled water, made into maximum concentration, and administered according to the maximum allowable capacity of mice. Dosing is followed by 18, 14.4, 11.5, 9.2, 7.4 (g.kg-1) according to the crude drug amount, and after the animal is fasted (can't help water) for 18 hours, it is divided into two administrations (interval half an hour) in one day, 0.5ml each time, observe the animal death.

2. Determination of maximum tolerated dose (MTD value): take 20 mice, 10 males and 10 males. The present invention is added with distilled water and dissolved to make the highest concentration. According to the maximum tolerance of the animal, the injection feeding device can twitch as the criterion. After the animals fasted (without water) for 18 hours, they were administered twice a day (with an interval of half an hour), 0.5ml each time (each ml contained 0.36g of crude drug), and the total drug dose was 18g of crude drug/kg.d, equivalent to 300 times the amount of clinical adult 50Kg body weight. Continuous observation for 7 days after administration.

3. Test results:

In the LD50 calculation, when the mice were administered with the maximum allowable concentration and the maximum allowable capacity (18g/Kg.d), no death of the mice was seen, that is, the LD50 was not measured, and only the maximum tolerated dose could be obtained. During the 7-day observation period , the animals' appetite, activity, coat color, mental state, etc. were all normal, and the development was normal, and no death was seen. That is, the dosage equivalent to 300 times of the clinical dose was selected, and no adverse reaction occurred, indicating that the acute toxicity was extremely small, and the MTD was greater than 18g/Kg.d.

Experimental example 2: Acute toxicity and long-term toxicity test results

Acute toxicity test: give the present invention by one-time gavage of mice, maximum concentration 35%, maximum gavage capacity 0.4ml/10g, dose 14g/kg (every g of powder is equivalent to 10g crude drug), observe continuously for 7 days, no For any toxic reaction, the LD 50 of the drug could not be measured because the concentration and dose could not be increased. Determination of the maximum tolerated dose: with the highest concentration and the maximum gavage volume, the mice were gavaged three times with an interval of 5 hours, and then observed continuously for 7 days, and no case died. The dosage of the medicinal powder is >42g/kg.day (each g of medicinal powder is equivalent to 10g of crude drug), which is equivalent to 420 times of the clinical daily dosage of an adult in terms of kilogram body weight.

Long-term toxicity test: in order to observe whether long-term medication produces toxic reactions, give rats the present invention to feed respectively, by 70 times and 35 times (being 7g/kg/ day and 3.5g/kg/ day) of adult's clinical daily dose, continuous After feeding for 8 weeks, there was no abnormality in the behavior, eating, and body weight of the rats. Compared with the control group, the blood routine, liver and kidney functions, and various important organs had no abnormal changes. Any toxic and side effects of the invention. Acute and chronic toxicity tests on animals prove that the invention has a large safety range and is a safe and reliable health care product.

Pharmacodynamics experiment:

Immunomodulatory effect

Using modern immunology theory and technology to study the effect of traditional Chinese medicine on enhancing and regulating the immune function of the body is of great significance for clarifying the material basis of the theory of traditional Chinese medicine. The invention is formed by scientific compatibility of several natural medicines such as mulberries. Existing studies have found that the oral liquid of the present invention can adjust the functions of various system organs of the body, regulate immune cells and immune molecules, and have a certain effect of improving immunity. Now further explore the molecular immunological mechanism of the oral liquid of the present invention on the immunomodulatory effect on immunocompromised mice with experiments.

1 Materials and methods

1.1 Instruments and reagents Oral liquid of the present invention is provided by the preparation department of this hospital; main raw materials are: mulberry, curculigo, rehmannia glutinosa, schisandra chinensis, raspberry, poria cocos, digupi, acanthopanax, Radix Polygoni Multiflori, dogwood, mulberry, Prepared by the method of Example 2. Cyclophosphamide for injection was provided by Shanghai Hualian Pharmaceutical Co., Ltd. Cytokine detection kits were purchased from American R&D Company. Flow cytometer, 2000FCA, produced by BD Company of the United States.

1.2 Animals Thirty Kunming male mice, 6-8 weeks old, weighing 25±2g, were provided by Shandong University Experimental Animal Co., Ltd.

1.3 Method

1.3.1 Establishment of animal model and grouping Kunming male mice were randomly divided into 3 groups: blank control group, model group and drug group, with 10 mice in each group. Blank control group: normal feeding without any treatment during the experiment; model group: intraperitoneal injection of 100 mg/kg cyclophosphamide to cause hypoimmunity model, once a day, for 3 consecutive days; drug group: intraperitoneal injection of 100 mg/kg cyclophosphamide Phosphoramide induced hypoimmunity model, 1 time/d, for 3 consecutive days, and smeared on the left tibialis anterior muscle of mice on the first day of modeling, 0.1mL/mouse, boosted immunization once a week later, the dose was the same as that of the first day once. Samples were collected after 4 days.

1.3.2 Determination of mouse immune organ index After weighing the body weight of the mice, the mice were killed by cervical dislocation, and the thymus and spleen were taken to weigh their wet weight, and the thymus index and spleen index were calculated:

Thymus index = thymus weight × 1000/body weight

Spleen index = spleen weight × 1000/body weight

1.3.3 Determination of IgG and IgM levels in mouse serum According to the kit instructions, the IgG and IgM levels in mouse serum were determined.

1.3.4 Determination of T lymphocytes and their subsets CD4+, CD8+ and CD4+/CD8+ in mouse spleen tissue Aseptically take the spleen, place it in a plate containing an appropriate amount of Hank's-free liquid, and gently grind the spleen with tweezers to prepare A single cell suspension was filtered through a 200-mesh sieve, the cells were suspended in 1 mL of complete culture medium, and the cell concentration was adjusted to 1×109L ^-1 , and 100 μL of well-mixed spleen cell suspension was carefully added with a micropipette. To the bottom of the injection tube, add 20 μL of monoclonal antibody, oscillate at low speed for 3 seconds, incubate at room temperature in the dark for 30 minutes, and after counterler-PREP breaks red blood cells, use a flow cytometer to detect the content of T cell subsets.

1.3.5 Determination of IFN-Y and IL- ¹⁰ levels in mouse serum According to the kit instructions, the levels of IFN-Y and IL- ¹⁰ in serum were measured.

1.2 Statistical processing All values are represented by x ± s, and SPSS13.0 statistical software is used for data analysis, and t test is used for comparison between groups. Take P<0.05 as the standard of statistically significant difference.

2 results

2.1 Effects on the immune organs of the immunocompromised mice It can be concluded from Table 1 that compared with the normal control group, the spleen and thymus indexes in the model group were significantly lower, and the difference was statistically significant, P<0.01. Compared with the model group, the spleen index of the mice in the drug group increased significantly, which was statistically significant, P<0.01, and the thymus index also increased, but the difference was not significant.

Table 1 The present invention is to the influence (n=10, mean ± S) of immunocompromised mice immune organ weight

Compared with normal control group: Δ: P<0.05, ΔΔ: P<0.01; compared with model group: *: P<0.05, **: P<0.01

2.2 Effects on T lymphoid subgroups in spleen tissue of immunocompromised mice It can be seen from Table 2 that compared with the normal control group, the percentage of CD4+ cell subsets in the spleen of the model group mice decreased, and the percentage of CD8+ cell subsets increased, with significant differences , P<0.05, the ratio of CD4+/CD8+ decreased significantly, with a very significant difference, P<0.01. However, the drug group can obviously counteract the effect of cyclophosphamide on destroying immunity, with a significant difference, P<0.05.

Table 2 Effect of the present invention on humoral T cell subsets in immunocompromised mice (n=10, mean±SD)

Compared with normal control group: ΔP<0.05, ΔΔP<0.05; compared with model group: *P<0.05, **P<0.05.

Thymus and spleen are important lymphatic organs of the body. The thymus is the place where T cells develop, differentiate and mature, and its structure and function are closely related to the state of the body. The reduction of thymus index and thymus atrophy are the manifestations of immunosuppression mainly of cellular immunity in mice. The spleen is the largest peripheral immune organ in the body. The immune cells of the spleen are mainly B lymphocytes, and B lymphocytes are the main cells involved in humoral immunity. Therefore, the spleen is closely related to the humoral immunity of the body. Thymus index and spleen index intuitively reflect the strength of immune body function. This experiment shows that the oral liquid of the present invention can promote the development of the spleen of mice with hypoimmunity caused by cyclophosphamide, and also have a certain promoting effect on the development of the thymus.

Modern medical research shows that the essence of aging is the decline of stem cells, the decline of immunity, the degradation of tissue and organ functions, and the decline of anti-aging genes; therefore, scientists believe that the most fundamental way to improve the immunity of the human body is to delay the aging of the human body process. Pursuing immortality and delaying life has been the dream of human beings for thousands of years, and human scientists are still studying the subject of human longevity unremittingly for a long time. This experiment proves from one aspect that the present invention can significantly improve the immunity level, and further effectively resist aging.

Traditional Chinese medicine believes that mulberries are sour in taste and slightly cold in nature. Sore knees, premature graying of beard and hair, quenching thirst and quenching dryness, dry stool, nourishing yin and blood, soothing the nerves and nourishing the brain, sharpening joints, eliminating rheumatism, hangover and other effects.

Mulberries contain fatty acids, mainly linoleic acid. Composed of stearic acid and oleic acid, it has the functions of decomposing fat, reducing blood fat, and preventing vascular sclerosis. Strengthen the spleen and stomach and help digestion: Mulberry fruit contains tannic acid, fatty acid, malic acid and other nutrients, which can help the digestion of fat, protein and starch, so it has the function of strengthening the spleen and stomach and helping digestion, and can be used to treat diarrhea caused by indigestion. Mulberry contains a large amount of water, carbohydrates, multivitamins, carotene and trace elements necessary for the human body, etc., which can effectively expand the blood volume of the human body, and nourish but not greasy. It is suitable for diet therapy for patients with hypertension and gynecological diseases. Contain the nutritive substance needed by a large amount of human body in the mulberry fruit, also contain black hair element, can make hair become black and glossy, can be used for beauty treatment. Ripe mulberries are rich in nutrients, containing 81.8 grams of water, 1.8 grams of protein and 0.3 grams of fat per 100 grams of mulberries. 4.9 grams of cellulose, 10 grams of carbohydrates, 1.2 grams of ash, 30 micrograms of carotene, 0.02 mg of thiamine, 0.06 mg of riboflavin, 6.95 mg of vitamin E, 33 mg of potassium, 0.27 mg of zinc, 0.08 mg of copper, selenium 4.8 micrograms. In addition, it also contains tannic acid, malic acid, vitamin C and fatty acids. Its fat is mainly linoleic acid, oleic acid, palmitic acid, stearic acid and a small amount of caprylic acid, nonanoic acid, capric acid, myristic acid, linolenic acid and so on. Add lung-nourishing gold, nourishing kidney water, astringe lung-qi, relieve cough and asthma, nourish kidney-essence, improve brain power, improve intelligence and strengthen body, and some mild-smelling traditional Chinese medicines have certain anti-aging effects on the human body. It is a health care that improves immunity. Taste. The present invention uses mulberries as the main ingredient, and adds some traditional Chinese medicines that nourish the liver and kidney, strengthen tendons and bones, promote blood circulation and nourish marrow, and have mild nature and flavor. It has greatly improved and has a strong market prospect; the combination of various medicines can nourish the lungs, nourish the kidneys, restrain the lungs, relieve cough and asthma, nourish the kidneys, and improve brain power, so it can improve the mind and strengthen the body, and has a certain anti-aging effect on the human body , is a health product that improves immunity.

Claims (10)

1. the preparation method of the Fructus Mori oral liquid improving immunity, it is characterised in that comprise the following steps:

A. the extraction of Fructus Mori, as component 1；

B. remaining crude drug described is put into heating and refluxing extraction 2 times in ethanol, as component 2；

C. by 2-in-1 also to above two extracting solution component 1 and component, add Mel after concentration or dextrin is in harmonious proportion, prepare after disinfection Become oral solutions.

Improve the preparation method of the Fructus Mori oral liquid of immunity the most according to claim 1, it is characterised in that described step a In, gather during annual 4-6 month Fructus Mori fruit maturation, clean, decontamination, dry after drying or slightly steaming.

Improve the preparation method of the Fructus Mori oral liquid of immunity the most according to claim 1, it is characterised in that described step a In, farther including, Fructus Mori good for dry in the sun are soaked and has been placed in lid stainless steel cask, add 70% ethanol, quality is coarse granule 0.8-1 times, stir, moistening airtight placement more than 1 hour, make fully to expand；By filter plate gauze bag bottom percolator Drug sample after moistening expansion is mixed pine and is done to dissipate by parcel again after paving, then contain powder with rustless steel spoon, load percolation uniformly Cylinder, fills 10-12 cm thick, even by T-bar pressure, then presses aforesaid operations, and loading in layer is suitably pressurizeed, and medicated powder loads not 2/3 eminence of percolator must be exceeded；Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts one Container, is then slowly added into 70% ethanol；Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close Close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material Outflow 2～3ml per minute, filtrate is put into liquid container, and is poured ethanol during emptying into liquid container；Percolate is merged quiet Put, stand for standby use, as component 1.

Improve the preparation method of the Fructus Mori oral liquid of immunity the most according to claim 1, it is characterised in that described step b In, the medicine of remaining crude drug is put in ethanol, soaks 1-2 days, then heating extraction 2 times, each 1-2 hour, 2 times are extracted Liquid merges standing；The medicinal residues of the raw medicinal material crossed by above-mentioned ethanol extraction again add water heating and refluxing extraction 2 times, and each 1～2 is little Time, 2 extracting solution are merged and stands；Above two extracting solution is merged, the filter of 1.35 when concentrating under reduced pressure relative density is 80 DEG C Liquid, reclaims ethanol, and by gained filtrate adjustment proportion to 1.38, reduce pressure to obtain extractum, becomes component 2.

Improve the preparation method of the Fructus Mori oral liquid of immunity the most according to claim 1, it is characterised in that described step c In, by 2-in-1 to component 1 and component and after, insert in economic benefits and social benefits vacuum concentrator, first concentrating the filtrate to relative density when 80 DEG C is The concentrated solution of 1.38, sets to 0～5 DEG C of deepfreezes 24 hours；The filter aid kieselguhr adding 0.3% by cold preservation liquid, filters, and filtrate is again Insert in economic benefits and social benefits vacuum concentrator, be concentrated into every 1ml extractum Han 0.1g, add dextrin and be in harmonious proportion, after disinfection, be prepared as oral liquid.

6. the preparation method of the Fructus Mori oral liquid improving immunity, it is characterised in that comprise the following steps:

A. the preparation of Fructus Mori, as component 1；

B. remaining crude drug described chopping is extracted as component 2；

C. component 1 and component 2 are mixed, add proper honey after concentration and be in harmonious proportion, bottle after ultraviolet ray sterilization bactericidal.

Improve the preparation method of the Fructus Mori oral liquid of immunity the most according to claim 6, it is characterised in that described step a In, the Fructus Mori that dry in the sun is good are steeped in ethanol after 1-2 days, heating extraction 2 times, each 1-2 hour, take supernatant, united extraction Liquid, 100-110 mesh filters, then the ultrafiltration post ultrafiltration that molecular weight is 6000-10000 through damming, and ultrafiltrate concentrating under reduced pressure is the closeest The extractum of 1.36 when degree is 80 DEG C, heating is concentrated into paste, stand for standby use, becomes component 1；In described step b, by remaining crude drug Material mixes, and shreds 100～120 mesh sieves, and after sieving, fine powder is standby, and coarse granule puts into acid and alkali-resistance infusion kettle, at room temperature, with 70 Ethanol more than spending impregnates 3～7 days together, then heating and refluxing extraction 2 times, each 1～2 hour, merges quiet by 2 extracting solution Put, filter, after separation, take filtrate；By filtered through gauze, adding 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued extraction 2h, often 10min stirs once, filtered through gauze, merges lixiviating solution, and being condensed into pasty state becomes component 2；In described step c, by component 1 and group Point 2-in-1 and after, insert in economic benefits and social benefits vacuum concentrator, when being concentrated into 80 DEG C, relative density is the extractum of 1.60, the unguentum after concentration Add Mel or dextrin is in harmonious proportion, bottle after ultraviolet ray sterilization bactericidal.

8. the Fructus Mori oral liquid improving immunity, it is characterised in that include the crude drug of following weight portion: Fructus Mori 10～50 Part, Rhizoma Curculiginis 10～20 parts, Radix Rehmanniae Preparata 10～20 parts, Fructus Schisandrae Chinensis 10～20 parts, Herba Taraxaci 10～20 parts, Poria 10～20 parts, ground bone Skin 10～20 parts, Cortex Acanthopancis 10～20 parts, Radix Polygoni Multiflori Preparata 10～20 parts, Fructus Corni 10～20 parts, Herba Taxilli 10～20 parts, Herb Gynostemmae Pentaphylli 10～20 parts, the Radix Astragali 10～20 parts, Herba Sedi 10～20 parts, Radix Codonopsis 10～20 parts, Fructus Ligustri Lucidi 10～20 parts, Semen Cuscutae 10～20 Part, Cortex Moutan 10～20 parts, Fructus Gardeniae 10～20 parts.

Improve the Fructus Mori oral liquid of immunity the most according to claim 1, it is characterised in that described Fructus Mori oral liquid also includes The crude drug of following weight portion: Fructus Mori 10～40 parts, Rhizoma Curculiginis 10～15 parts, Radix Rehmanniae Preparata 10～15 parts, Fructus Schisandrae Chinensis 10～15 parts, Pu Gong English 10～15 parts, Poria 10～15 parts, Cortex Lycii 10～15 parts, Cortex Acanthopancis 10～15 parts, Radix Polygoni Multiflori Preparata 10～15 parts, Fructus Corni 10 ～15 parts, Herba Taxilli 10～15 parts, Herb Gynostemmae Pentaphylli 10～20 parts, the Radix Astragali 10～20 parts, Herba Sedi 10～20 parts, Radix Codonopsis 10～20 Part, Fructus Ligustri Lucidi 10～20 parts, Semen Cuscutae 10～20 parts, Cortex Moutan 10～20 parts, Fructus Gardeniae 10～20 parts.

Improve the Fructus Mori oral liquid of immunity the most according to claim 1, it is characterised in that described Fructus Mori oral liquid also wraps Include the crude drug of following weight portion: Fructus Mori 10～30 parts, Rhizoma Curculiginis 10～20 parts, Radix Rehmanniae Preparata 10～20 parts, Fructus Schisandrae Chinensis 10～20 parts, Pu Herba Taraxaci 10～20 parts, Poria 10～20 parts, Cortex Lycii 10～20 parts, Cortex Acanthopancis 10～20 parts, Radix Polygoni Multiflori Preparata 10～20 parts, Fructus Corni 10～20 parts, Herba Taxilli 10～20 parts, Herb Gynostemmae Pentaphylli 10～15 parts, the Radix Astragali 10～15 parts, Herba Sedi 10～15 parts, Radix Codonopsis 10～15 Part, Fructus Ligustri Lucidi 10～15 parts, Semen Cuscutae 10～15 parts, Cortex Moutan 10～15 parts, Fructus Gardeniae 10～15 parts.