CN108882689A - 烟草植物体及其制备方法 - Google Patents

烟草植物体及其制备方法 Download PDF

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CN108882689A
CN108882689A CN201780021047.8A CN201780021047A CN108882689A CN 108882689 A CN108882689 A CN 108882689A CN 201780021047 A CN201780021047 A CN 201780021047A CN 108882689 A CN108882689 A CN 108882689A
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铃木庄
铃木庄一
浜野香织
佐藤正纪
新井雅雄
根岸勇太
野村绫子
塚原麻伊
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Japan Tobacco Inc
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Abstract

本发明提供适于为了收获烟叶而栽培的烟草植物体。本发明涉及导入了抑制腋芽发育的突变的烟草植物体、以及该烟草植物体的制备方法。

Description

烟草植物体及其制备方法
技术领域
本发明涉及适于为了收获烟叶而栽培的烟草植物体及其制备方法。
背景技术
种子植物在其生长过程中，种子的胚发育而形成子叶及芽顶端分生组织(shootapical meristem)。通过上述芽顶端分生组织的细胞分裂，逐步形成叶原基(leafprimordium)，在叶原基的近轴侧(adaxial side)形成腋生分生组织(axillarymeristem)。腋生分生组织由此作为芽顶端分生组织而发挥功能，成为腋芽(axillaryBud)。在植物体的营养生长时，腋芽的发育通常暂时处于休眠状态(受到了抑制)。初生茎(primary shoot)的芽顶端分生组织在从营养生长状态转变为生殖生长状态时、或者在死亡时，腋芽的发育从休眠状态苏醒，受到促进。对于腋芽的发育，在茄科植物(例如，番茄及烟草)、其它植物(例如，稻及拟南芥)中有多项研究报道。
作为用于收获叶的栽培植物的烟草植物，在其栽培中，为了提高收获叶的质和量(例如，收获叶的内含成分的积累、成熟及展开)而实施打顶(将带有花的顶端部的茎切除)。实施打顶后，烟草植物从叶的根部(叶腋)等开始旺盛的腋芽发育。腋芽的发育当然需要消耗营养成分，因此供给至收获叶的营养成分相对减少。由此，腋芽的发育及生成会导致收获叶的品质及产量的降低。出于与打顶相同的原因，在从打顶至收获叶的期间，对腋芽进行除去或抑制发育等处理。需要说明的是，至少对于烟草植物而言，抑制即使暂时除去腋芽，腋芽也会从相同的叶的根部反复发育，在用于收获烟叶的烟草植物的栽培中，腋芽的处理是有待解决的重要问题。
作为除去腋芽的方法，可以举出通过手工操作或机械进行摘取。通过手工操作进行摘取存在需要巨大的劳动力(及与此相伴的人工费的增加)、以及效率低的问题。利用机械进行摘取存在操作的准确率比手工操作差而损伤植物体的问题。另外，作为抑制腋芽发育的方法，可以举出使用农药及利用基因修饰进行抑制。农药的使用存在为了保持效果而反复散布、对植物体的生长造成影响、因农药残留而对收获叶造成影响、以及残留农药的检测成本增大的问题。
这里，关于腋芽的发育，在专利文献1、2及非专利文献1～19中有所公开。专利文献1及2公开了用于抑制腋芽的发育的技术。
以下，基于非专利文献1～19，对于与腋芽的原基形成相关的基因进行说明。
作为与腋芽的原基形成相关的基因，有来自除烟草以外的植物的多个基因的报道。作为代表性的基因，可以列举：LATERAL SUPPLESSOR(LS)、Blind(Bl)、REVOLUTA(REV)、CUP-SHAPED COTYLEDON(CUC)。
已经报道了从拟南芥(非专利文献1)、番茄(非专利文献2)、稻(非专利文献3)中分离LS，其是腋芽原基形成的必要基因。在拟南芥的LS基因的突变体中，茎性叶的腋芽是正常的，但除了上部2片以外的莲座叶基本上观察不到腋芽(非专利文献1)。在番茄的LS基因的突变体中，营养生长期(vegetative stage)的腋芽消失，但在生殖生长期(reproductivestage)中形成了上部2个部位的腋芽(非专利文献2)。在稻的LS基因的突变体(moc1)中，在营养生长期(tillering stage)、生殖生长期(heading stage)中均完全观察不到与稻的腋芽相当的分蘖(tiller)(非专利文献3)。对于烟草而言，虽然已经公开了推测为LS直向同源基因(ortholog gene)的cDNA序列(收录号：EU0935581.1)，但尚未在烟草中对该基因的功能进行确认。
Bl的基因从拟南芥(非专利文献4及5)、番茄(非专利文献6)中分离。在番茄中，由于1个基因的突变体，在进行了摘心的情况下，也与叶位无关地基本上不形成腋芽(非专利文献6及7)。在拟南芥中，作为Bl直向同源，报道了至少3个功能重复的基因(REGULATOR OFAXILLARY MERISTEM(RAX)1、2及3)。即使是RAX1单独突变体也观察到了抑制，在3重突变体中，在莲座叶基本上不形成腋芽，茎性叶的腋芽也大幅减少(非专利文献4及5)。在RAX1单独突变体中，即使在切除初生茎之后，也没有观察到从未见到形成腋芽的下部莲座叶生长腋芽(outgrowth)。根据拟南芥的RAX基因序列、以及从葡萄及番茄的基因组序列推测出的推定氨基酸序列的相同性比较，可以推测拟南芥的RAX1的番茄的直向同源基因除了Blind以外还有C基因。但是，C基因不是与腋芽的形成、而是与叶的形成相关的基因(非专利文献8)。在烟草中，没有推测为Bl直向同源基因的cDNA序列的报道，但公开了与番茄Bl的氨基酸序列93％相同的EST序列(收录号：FS402940.1)。然而，烟草中该基因的功能尚未报道。
REV的基因从拟南芥中分离(非专利文献10及11)，REV的突变体在莲座叶及茎性叶两者中腋芽原基的形成减少，未观察到通过摘心(decapitation)而促进腋芽原基形成(非专利文献9、10及12)。在烟草中没有推测为REV直向同源基因的cDNA序列的报道，但公开了在氨基酸水平上与拟南芥REV有79％相同的EST序列(收录号：FG135778.1)。另外，公开了推测为REV直向同源基因的烟草(品种：SR-1)的全长cDNA序列(收录号：JQ686937)。但是，烟草中与REV相同性高的基因的功能尚未报道。
CUC的3个基因(CUC1、2及3)从拟南芥中分离(非专利文献16～18)，CUC1和2均对茎顶端分生组织的调节发挥功能，在功能上重复(非专利文献15)。在CUC3基因单独的突变体中观察到莲座叶的腋芽形成受到抑制，但在CUC2及3的二重突变体中，腋芽抑制程度不大(非专利文献13及14)。在烟草中，尚未报道推测为CUC直向同源基因的cDNA序列，但公开了与作为拟南芥CUC1基因的保守结构域的NAM结构域序列的氨基酸序列81％相同的EST序列(FG644078.1)。另外报道了，在使用了推测为罗布红麻(Apocynum venetum)的CUC3的序列的RNAi重组烟草中，烟草的某个基因(未公开序列)的表达受到抑制，观察到了与拟南芥的CUC突变体类似的叶的形态异常(非专利文献19)。但是，在烟草中，对于与CUC相同性高的基因的功能尚不明确，至少没有对于腋芽的功能的报道。
先行技术文献
专利文献
专利文献1：美国专利申请公开第2009/0249518(2009年10月1日公开)
专利文献2：国际公开第2010/081917号小册子(2010年7月22日公开)非专利文献
非专利文献1：Greb T,Clarenz O,Schafer E,Muller D,Herrero R,Schmitz G,Theres K(2003)Molecular analysis of the LATERAL SUPPRESSOR gene inArabidopsis reveals a conserved control mechanism for axillary meristemformation.Genes Dev.17：1175-1187
非专利文献2：Schumacher K,Schmitt T,Rossberg M,Schmitz G,Theres K(1999)The Lateral suppressor(Ls)gene of tomato encodes a new member of theVHIIDprotein family.Proc Natl Acad Sci USA 96：290-295
非专利文献3：Xueyong L,Qian Q,Zhiming F,Yonghong W,Guosheng X,Dali Z,Xiaoqun W,Xinfang L,Sheng T,Fujimoto H,Ming Y,Da L,Bin H&Jiayang L(2003)Control of tillering in rice.Nature 402(10)：618-621
非专利文献4：Keller,T.,Abbott,J.,Moritz,T.,and Doerner,P(2006)Arabidopsis REGULATOR OF AXILLARY MERISTEMS1 controls a leaf axil stem cellnicheand modulates vegetative development.The Plant Cell 18：598-611
非专利文献5：Muller D,Schmitz G,Theres K(2006)Blind homologous R2R3Myb genes control the pattern of lateral meristem initiation inArabidopsis.The Plant Cell 18：586-597
非专利文献6：Schmitz G,Tillman E,Carriero F,Fiore C,Cellini F,TheresK(2002)The tomato Blind gene encodes a MYB transcription factor thatcontrolsthe formation of lateral meristems.Proc Natl Acad Sci USA 99：1064-1069
非专利文献7：Mapelli SC,Lombardi L(1982)A comparative auxin andcytokinin study in normal and to-2 mutant tomato plants.Plant CellPhysiol.23：751-757
非专利文献8：Busch BL,Schmitz G,Rossmann S,Piron F,Ding J,BendahmaneA,Theres K(2011)Shoot branching and leaf dissection in totamto are regulatedbyhomologous gene modules.The Plant Cell 23：3595-3609
非专利文献9：Talbert PB,Adler HT,Parks DW,Comai L(1995)The REVOLUTAgene is necessary for apical meristem development and for limiting celldivisions in the leaves and stems of Arabidopsis thaliana.Development 121：2723-2735.
非专利文献10：Otsuga D,DeGuzman B,Prigge MJ,Drews GN,Clark SE(2001)REVOLUTA regulates meristem initiation at lateral positions.The Plant Journal25：223-236
非专利文献11：Zhong R,Ye ZH(1999)IFL1,a gene regulatinginterfascicular fiber differentiation in Arabiodpsis,encodes a homeodomain-leucine zipper protein.The Plant Cell 11：2139-2152
非专利文献12：Zhong R,Taylor JJ,Ye ZH(1997)Disruption ofinterfascicular fiber differentiation in an Arabidopsis mutant.The Plant Cell9：2159-2170
非专利文献13：Hibara K,Karim MR,Takada S,Taoka K,Furutani M,Aida M,Tasaka M(2006)Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonicshootmeristem and organ boundary formation.The Plant Cell 18：2946-2957
非专利文献14：Raman,S.,Greb,T.,Peaucelle,A.,Blein,T.,Laufs,P.andTheres,K(2008)Interplay of miR164,CUP-SHAPED COTYLEDON genes andLATERALSUPPRESSOR controls axillary meristem formation in Arabidopsisthaliana.The Plant Journal 55：65-76
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发明内容
发明要解决的课题
然而，根据上述文献可知，除了烟草植物以外，仅能够减少腋芽。因此，如何获得消除或减轻了腋芽的发育所导致的问题的、用于收获烟叶而栽培的烟草植物尚不明确。
本发明的课题在于提供一种适于为了收获烟叶而栽培的烟草植物体。
解决课题的方法
鉴于上述课题，本发明人等对烟草植物中预想与腋芽发育相关的基因进行鉴定，对于由该基因表达的蛋白质在烟草植物体中存在量减少的情况下可获得的有利影响进行了探索，结果完成了本发明。
即，为了解决上述课题，本发明的烟草植物体在基因组中导入了引起基因的功能抑制的突变，所述基因包含编码以下(1)～(5)的多肽中任一者的多核苷酸作为编码区域，
其中，所述(1)～(5)的多肽为：
(1)相对于序列号1所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号3所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(2)相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(3)相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(4)相对于序列号13所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号15所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；以及
(5)相对于序列号17所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号19所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者，
所述功能抑制抑制腋芽的发育。
为了解决上述课题，本发明的烟草植物体的制备方法包括导入引起基因的功能抑制的突变的步骤，所述基因包含编码了以下(1)～(5)的多肽中任一者的多核苷酸作为编码区域，
其中，(1)～(5)的多肽为：
(1)相对于序列号1所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号3所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(2)相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(3)相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(4)相对于序列号13所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号15所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；以及
(5)相对于序列号17所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号19所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者，
所述功能抑制抑制腋芽的发育。
发明的效果
本发明能够选择性抑制腋芽发育，因此起到能够提供适于为了收获烟叶而栽培的烟草植物体的效果。
附图说明
图1是示出了确认本发明的实施例的NtREV基因的表达抑制对腋芽发育的影响的结果的图。
图2是示出了确认本发明的实施例的NtBl1基因的表达抑制对腋芽发育的影响的结果的图。
图3是示出了确认本发明的实施例的NtLS基因的表达抑制对腋芽发育的影响的结果的图。
图4是示出了确认本发明的实施例的#15360基因的表达抑制对腋芽发育的影响的结果的图。
图5是示出了确认本发明的实施例的#07437基因的表达抑制对腋芽发育的影响的结果的图。
图6是示出了确认本发明的实施例的在NtREV基因中导入的突变对腋芽发育的影响的结果的图。
图7是示出了确认本发明的实施例的在NtLS基因中导入的突变对腋芽发育的影响的结果的图。
图8是示出了为了利用CRISPR/Cas9system在NtBl1基因中导入突变而使用的载体的结构的示意图。
图9是示出了确认本发明的实施例的在NtBl1基因中导入的突变对腋芽发育的影响的结果的图。
图10是示出了确认本发明的实施例的在NtBl1基因中导入的突变对腋芽发育的影响的结果的图。
图11是示出了确认本发明的实施例的在NtBl1基因或NtLS基因中导入的突变对腋芽的发育位置的影响的图。
图12是示出了确认本发明的实施例的在NtBl1基因中导入的突变对烟草植物的生长的影响的图。
图13是示出了确认本发明的实施例的在NtLS基因中导入的突变对烟草植物的生长的影响的图。
图14是示出了确认在NtREV基因中导入的突变对烟草植物的生长的影响的图。
图15是示出了确认在LS基因中导入的突变对烟草植物的生长的影响的图。
图16是示出了确认在LS基因中导入的突变对腋芽发育的影响的结果的图。
图17是示出了确认在REV基因中导入的突变对腋芽发育的影响的结果的图。
图18是示出了确认在LS基因中导入的突变对腋芽发育的影响的结果的图。
图19是示出了确认在REV基因中导入的突变对腋芽发育的影响的结果的图。
图20是示出了确认在REV基因中导入的突变对腋芽发育的影响的结果的图。
图21是示出了确认在#15360中导入的突变对腋芽发育的影响的结果的图。
图22是示出了确认在Bl1基因中导入的突变对腋芽发育的影响的结果的图。
图23是示出了确认在Bl1基因中导入的突变对腋芽发育的影响的结果的图。
图24是示出了确认在Bl1基因中导入的突变对腋芽发育的影响的结果的图。
图25是示出了确认在LS基因中导入的突变对腋芽发育的影响的结果的图。
图26是示出了确认在LS基因中导入的突变对腋芽发育的影响的结果的图。
具体实施方式
〔1.烟草植物体〕
在一个方面，本发明提供一种烟草植物体，其在基因组中导入了引起基因的功能抑制的突变，所述基因包含编码了特定多肽的多核苷酸作为编码区域。这里，该功能抑制是抑制腋芽的发育。
特定的上述多肽的具体例子为以下的任一者：相对于序列号1所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号3所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；相对于序列号13所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号15所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；以及，相对于序列号17所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号19所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者。
如后述的实施例所示，上述烟草植物体在一级腋芽的数量或重量方面没有显示出与野生型的实质性差异，但是除去了一级腋芽之后生出的腋芽(二级腋芽及三级腋芽)的数量或重量显示出大幅减少(例如，野生株植物的1/2以下)，或者在除去了一级腋芽之后不进一步产生腋芽。因此，本发明中从烟草植物体中除去腋芽的操作基本上1次结束，因此与用于收获烟叶的烟草植物体栽培中的现有的腋芽处理相比，只要几分之一以下的劳动力就可以完成。
如上所述，现有技术的文献中仅公开了除烟草植物以外，腋芽的发育整体上受到抑制。但是，从以下所述的原因考虑，整体上抑制腋芽的发育未必只有优点。对于种子植物，腋芽的发育能力是用于保持个体健全的重要功能。例如，在生长中的组织受到损伤的情况下，腋芽代替其开始生长，从而使个体实现自身的生存。因此，必然可以预见全面损害该功能会对个体的健全性带来风险。实际上，在非专利文献9(Fig.1的legend)中提及了使植物体的成长部分衰弱。另外，使腋芽完全消失的个体在因风灾水害等受到损伤时，有很高的死亡风险。因此，考虑到烟叶生产，腋芽的发育根据情况有重要的意义。在生长过程中初生茎损伤的情况下，通过使下位节的腋芽代替初生茎而伸长及发育，可以确保烟叶生产量。
在本说明书中使用的情况下，“烟草植物体”及“烟草”包含个体整体(例如，成体、苗及种子)、组织(例如，叶、茎、花、根、生殖器官、胚及它们的一部分等)、以及这些的干燥物。
在本说明书中使用的情况下，“腋芽(axillary bud)”是指在叶原基(leafprimordium)的叶腋(leaf axil)部形成的腋生分生组织(axillary meristem)所生成的芽(bud)及该芽发育而成的苗两者。腋芽在打顶后按照一级腋芽(primary axillary bud)、二级腋芽(secondary axillary bud)及三级腋芽(tertiary axillary bud)的顺序在相同叶的根部发育。首先，在打顶后一级腋芽发育，除去一级腋芽后，二级腋芽接着发育。腋芽的“发育”是指，停留在由上述腋生分生组织分化而成的状态的腋芽因茎顶的除去等而开始活泼的活动，进行生长及伸长。
腋芽的“数量或重量”是指1个个体中发育的腋芽的数量或采集到的腋芽的总质量(新鲜重量)，也适用于一级腋芽、二级腋芽或三级腋芽。
在本说明书中使用的情况下，“(氨基酸序列的)序列同一性”是指，相对于作为基准的(氨基酸)序列，提及的(氨基酸)序列一致的比例。这里，序列不一致的部分是存在(氨基酸残基的)取代、添加、缺失或插入的部分。
这里，使用序列表中记载的氨基酸序列确定多肽的记载“相对于～所示的氨基酸序列具有90％以上的序列同一性的多肽”是指野生型多肽。该野生型多肽是指通常存在于后述的烟草属植物中的多肽。
因此，本发明的烟草植物体中存在量减少了的上述特定多肽只要是与序列表所示的各氨基酸序列具有90％以上的序列同一性的多肽即可，该序列同一性优选为更高的比例(例如，91％、92％、93％、94％、95％、96％、97％、98％或99％以上)。
多肽的“存在量的减少”是指，以野生型多肽的存在量作为基准，存在70％以下、60％以下、50％以下、40％以下、30％以下、20％以下、10％以下、5％以下或1％以下的该多肽。以上述野生型多肽的存在量作为基准的上述多肽的存在量可以从带来二级腋芽的数量或重量减少的结果的上述值中适当选择。
需要说明的是，本发明的烟草植物体中的上述多肽存在量的减少优选在由该烟草植物体得到的培养细胞、愈伤组织、原生质体、种子及后代中遗传稳定地得到继承。因此，本发明的烟草植物体可以是由借助人工操作生成的培养细胞、愈伤组织、原生质体、种子及后代产生的个体，用于得到该个体的这些材料也包含于本发明的范围。
本发明的烟草植物体可以进一步包含通过交配而得到的育种后代。以稻、小麦、大麦、大豆为代表，在多个植物类种中进行了使用突变体的育种。例如，在从用突变原进行了处理的突变体群中分离出的突变体的情况下，除了目标基因以外，也有多个突变。因此，通常为了除去多余的突变而进行回交。此时，通过与具有优良性状的栽培品种进行交配，将突变体所具有的性状导入栽培品种，由此可以得到具有更高附加值的栽培品种。由于突变体所具有的性状来自于突变，因此为了进行回交，需要对具有突变的个体进行筛选。为了进行高效的回交，需要简便地检测有无突变和突变是纯合型还是杂合型的方法。该方法可以使用后述的检测突变的方法来进行。另外，通过使用在突变体与栽培品种之间显示出多态性的背景标志物进行MAS(Marker Assisted Selection)，可以以较少的杂交次数高效地得到返回栽培品种的比率高的品系。作为多态性标志物，可以利用烟草中公知的SNP、SSR(Simple Sequence Repeat)。如果需要，可以对待使用的烟草的基因组序列进行解读，确定碱基序列的区别、重复序列数的区别，从而获得并利用新的多态性标志物。
在本说明书中使用的情况下，“基因的功能抑制”是指位于基因组上的基因不发挥原本的功能的状态。因此，“基因的功能抑制”是包括“基因的破坏”、“基因的突变”及除该基因以外(包含外来基因)导致的该“基因的表达抑制”的用语。
在以下的说明中，关于基因及基因组，以Nicotiana tabacum(红花烟草、N.tabacum)为基准进行说明。作为以下说明的基准的Nicotiana tabacum(N.tabacum)，是双二倍体，有来自于作为祖先种的Nicotiana sylvestris(林烟草)的基因组(S基因组)及来自于Nicotiana tomentosiformis(绒毛状烟草)的基因组(T基因组)这两者。在N.tabacum中，由同一名称表示的基因在多数情况下是在各基因组中分别存在的二倍体，因此，该基因在S基因组中有2个等位基因，在T基因组中有2个等位基因。即，在N.tabacum的基因组上，多数情况下存在4个由同一名称表示的基因。
需要说明的是，对于烟草植物体，编码在物种之间实质上发挥相同功能的蛋白质的基因的一部分(不是全部)的、编码区域中的碱基序列在栽培品种之间可以有1～数％的差异，在栽培品种及近缘野生种之间可以有约10％以下的差异。
“基因的破坏”是指，基因组上不存在原本具有的基因、或者不能由位于基因组上的基因生成转录产物。“基因的突变”是指，不能生成原本的功能性蛋白质的基因的突变、虽然可生成蛋白质但使生成的量降低的基因的突变、或者虽然可生成蛋白质但使蛋白质的稳定性降低的基因的突变。“基因的表达抑制”是指，虽然该基因中未发生变化，但基因的功能(从转录为mRNA至后续翻译为蛋白质)经由其它因子被修饰而使蛋白质的生成量降低、或者处于不发生蛋白质生成的状态。“基因的表达抑制”例如可以通过由该基因转录的mRNA的分解而产生。
在本说明书中使用的情况下，“突变”具有本申请的技术领域中通常理解的含义，例如是指位于野生型的基因组上的碱基、或者位于野生型蛋白质的氨基酸残基的变化(例如，取代、缺失、插入、添加、重复或倒位等)。位于基因组上的碱基的变化除了以上的例子以外，还包括多个碱基的易位。
相对于序列号1、3、5、7、9、11、13、15、17或19所示的氨基酸序列具有90％以上的序列同一性的多肽是存在于野生株植物的多肽(或其变体)。因此，本发明的烟草植物体的上述多肽的存在量比野生株植物有所减少，因此比野生株植物所具有的上述功能更差。该功能例如为野生株植物中形成腋生分生组织的功能、从腋生分生组织分化为腋芽的功能、或者保持或提高腋芽的发育能力的功能。
具有序列号1所示的氨基酸序列的多肽例如由具有序列号2所示的碱基序列的多核苷酸所编码。具有序列号3所示的氨基酸序列的多肽例如由具有序列号4所示的碱基序列的多核苷酸所编码。这些多核苷酸符合后述的实施例所示的NtREV基因的cDNA，序列号2示出了S基因组的NtREV基因的cDNA序列，序列号4示出了T基因组的NtREV基因的cDNA序列。序列号21及22分别示出了NtREV基因的S基因组及T基因组中的碱基序列。序列号54及55分别示出了NtREV基因(包含5’上游及3’下游)的S基因组及T基因组中的碱基序列。
具有序列号5所示的氨基酸序列的多肽例如由具有序列号6所示的碱基序列的多核苷酸所编码。具有序列号7所示的氨基酸序列的多肽例如由具有序列号8所示的碱基序列的多核苷酸所编码。这些多核苷酸符合后述的实施例所示的NtLS基因的cDNA，序列号6示出了S基因组的NtLS基因的cDNA序列，序列号8示出了T基因组的NtLS基因的cDNA序列。序列号23及24分别示出了NtLS基因是S基因组及T基因组中的碱基序列。序列号56及57分别示出了NtLS基因(包含5’上游及3’下游)的S基因组及T基因组中的碱基序列。
具有序列号9所示的氨基酸序列的多肽由例如具有序列号10所示的碱基序列的多核苷酸所编码。具有序列号11所示的氨基酸序列的多肽由例如具有序列号12所示的碱基序列的多核苷酸所编码。这些多核苷酸符合后述的实施例所示的NtBl1基因的cDNA，序列号10示出了S基因组的NtBl1基因的cDNA序列，序列号12示出了T基因组的NtBl1基因的cDNA序列。序列号25及26分别示出了NtBl1基因的S基因组及T基因组中的碱基序列。序列号58～61分别示出了NtBl1基因(包含5’上游及3’下游)的S基因组及T基因组中的碱基序列。
具有序列号13所示的氨基酸序列的多肽由例如具有序列号14所示的碱基序列的多核苷酸所编码。具有序列号15所示的氨基酸序列的多肽由例如具有序列号16所示的碱基序列的多核苷酸所编码。这些多核苷酸符合后述的实施例所示的#15360的cDNA，序列号14示出了S基因组的#15360的cDNA序列，序列号16示出了T基因组的#15360的cDNA序列。序列号27及28分别示出了#15360的S基因组及T基因组中的碱基序列。序列号62及63分别示出了#15360(包含5’上游及3’下游)的S基因组及T基因组中的碱基序列。
具有序列号17所示的氨基酸序列的多肽由例如具有序列号18所示的碱基序列的多核苷酸所编码。具有序列号19所示的氨基酸序列的多肽由例如具有序列号20所示的碱基序列的多核苷酸所编码。这些多核苷酸符合后述的实施例所示的#07437的cDNA，序列号18示出了S基因组的#07437的cDNA序列，序列号20示出了T基因组的#07437的cDNA序列。序列号29及30分别示出了#07437的S基因组及T基因组中的碱基序列。
需要说明的是，上述#07437基因从序列相同性方面可以认为是分类为CUC的基因。CUC基因在拟南芥中已经报道了CUC1～3这3个基因，还已知对于突变体的表型而言，集成了多个突变时比单独的突变显示出更大的影响。作为该家族基因，我们从烟草中除#07437以外还分离了5种基因。由此，可以期待通过将这些家族基因和#07437集成利用而发挥更大的效果。
在本发明的烟草植物体中，优选减少了特定的上述多肽的存在量。具体而言，该存在量通过编码上述野生型多肽的基因的突变或破坏、或者表达抑制而减少。
上述基因的突变或破坏例如作为自然突变、突变原处理、基因组编辑或基因敲除的结果而产生。上述基因的自然突变通常由复制错误及基因损伤而发生。该损伤的原因为暴露于天然存在的、或者人工生成后停留在自然环境中的公知的突变原(例如，放射线、紫外线或诱变物质(例如EMS等))等。上述基因的突变原处理可以通过人为地使上述突变原作用于烟草植物体(以及根据需要与抑制基因修复功能组合)来实施。上述基因的重组可以通过按照公知的基因重组方法，用重组序列对目标基因的一部分或全部进行同源重组来实施。上述基因的基因组编辑可以通过公知的技术(例如，zinc-finger nucleases：ZFN、transcription activator-like effector nucleases：TALEN、以及CRISPR/Cas9 system等)来实施。上述基因敲除可以通过利用了公知的转座酶的基因的转移或T-DNA的插入来实施。
如上所述，烟草植物体大多在T基因组及S基因组中具有2组基因。因此，为了使该基因的功能完全消失，需要使位于T基因组及S基因组的全部基因(4个)的功能缺失。但是，在表现出基因剂量效应(dosage effect)的情况下，即使不使T基因组及S基因组的功能缺失，也能够抑制上述基因的功能。
在通过取代使功能缺失的情况下，该取代可以存在于启动子序列(可以列举以编码区域为基准位于上游(5’侧)的序列、及位于下游(3’侧)的序列)、5’非翻译区域及3’非翻译区域、位于内含子两端的保守序列(5’GT-AG3’)、以及编码区域中的至少1个。可以预想，由于在位于启动子序列、5’非翻译区域及3’非翻译区域的对基因表达活性调节很重要的碱基序列中发生取代，因此由该基因的转录活性、稳定性引起转录产物量降低，蛋白质的量减少。在内含子的保守序列中发生了取代的情况下，不会正常发生剪接，而以添加内含子的形式进行翻译。因此可以预想，通过移码突变，可翻译出具有与原来不同的氨基酸序列的蛋白质。作为在编码区域发生了取代的情况的一例，可以预想，由于取代为不编码氨基酸的终止密码子(无义突变)而翻译出C末端侧变短的不完全长度的蛋白质，从而使功能缺失。无义突变的插入位置只要是无法形成完全长度的蛋白质的位置即可，优选缩短数个以上的氨基酸。
另一方面，可以预想，通过氨基酸序列被取代，蛋白质的功能降低。可以预想，这是由于氨基酸序列的取代，立体结构发生变化、对于活性功能重要的氨基酸变为不具有活性的氨基酸而引起的。非保守性取代(non-conservative substitutions)容易发生功能降低，因此优选作为氨基酸取代。作为非保守性取代，可以列举取代为电荷或疏水性不同的氨基酸(例如，由碱性氨基酸取代为酸性氨基酸、由极性氨基酸取代为非极性氨基酸)、以及取代为氨基酸侧链的位阻(立体上的大小)不同的氨基酸等。
在缺失、插入等除取代以外的突变的情况下，只要在启动子序列、5’非翻译区域及3’非翻译区域内发生，就可以预想与取代同样地因转录活性、稳定性的降低而引起转录产物量的减少、蛋白质的量的减少。即使是内含子的保守序列，也可以预想与取代同样地翻译出具有与原来不同的氨基酸序列的蛋白质。在编码区域发生的情况下，可以预想，由于氨基酸残基的缺失或插入(由3的倍数的连续的碱基缺失或插入而产生)、或者移码突变，翻译出具有与原来不同的氨基酸序列的蛋白质。另外，在包含全部该基因的大的缺失、大片段插入的情况下，可以预想该基因本身的表达会消失。
作为上述基因的突变或破坏的结果而产生的个体，在本说明书中称为烟草植物体的突变体(简单记载为突变体)。上述突变体可以在S基因组及T基因组中的任一者中具有上述的突变，优选在S基因组及T基因组两者中具有上述的突变。需要说明的是，用于使功能缺失的突变可以在1个基因中具有1种突变，也可以具有多个突变，并且突变的种类不受限制。S基因组及T基因组各有2个、总计4个基因的突变可以相同，也可以不同。
上述基因的表达抑制包括从该基因向mRNA转录的抑制、从该基因向介导mRNA的蛋白质的翻译的抑制(例如，该mRNA的分解)、以及翻译出的蛋白质的功能的抑制。转录的抑制可以通过抑制促进从上述基因进行转录的转录因子、以及抑制转录起始因子接近上述基因等来实现。翻译的抑制可以使用反义RNA分子、RNAi分子或共抑制分子来实现。蛋白质功能的抑制可以通过与功能性蛋白质结合而抑制该蛋白质的功能的分子(例如，诱饵(decoy)核酸、核酶、抗体及抑制肽)来实现。
以上说明的各种突变可以由参考了例如以下所示的各基因的公知的基因组序列、以及序列号54～63所示的各基因的基因组序列的本领域技术人员容易地导入烟草植物体。即，基于这些序列信息，可以适当确定本发明的概念所包含的各种烟草植物体的基因组中存在的、应导入突变的区域。
NtREV：(S基因组)Sol Genomics Network(SOL)accession#Ntab-K326_AWOJ-SS17907、及(T基因组)SOL accession#Ntab-K326_AWOJ-SS9429
NtLS：(S基因组)SOL accession#Ntab-K326_AWOJ-SS1238、及(T基因组)SOLaccession#Ntab-K326_AWOJ-SS5309
NtBl1：(S基因组)SOL accession#Ntab-K326_AWOJ-SS18396、及(T基因组)SOLaccession#Ntab-K326_AWOJ-SS12956
#15360：(S基因组)SOL accession#Ntab-K326_AWOJ-SS587及(T基因组)SOLaccession#Ntab-K326_AWOJ-SS20471
#07437：(S基因组)SOL accession#Ntab-K326_AWOJ-SS943、以及(T基因组)GeneBank accession#AYMY01348769.1、AWOK01667329.1及ASAG01052465.1。
上述的抑制(转录、翻译及蛋白质功能)可以通过例如将用于实现该抑制的分子直接导入植物体、或者通过使用插入了目标基因的载体的植物体的转化而使该分子在植物体中表达来实现。在植物体的转化中，用于表达上述分子的目标基因可以导入上述植物体的基因组的任意区域，不必导入S基因组及T基因组两者。
上述烟草植物体只要是烟草属植物即可，没有特别限定，作为烟草属植物，只要是属于烟草(Nicotiana)属的植物即可，没有特别限定，可以例如例如：无茎烟草(Nicotianaacaulis)、渐尖叶烟草(Nicotiana acuminata)、渐尖叶烟草多花型变种(Nicotianaacuminata var.multzjlora)、非洲烟草(Nicotiana africana)、花烟草(Nicotianaalata)、抱茎烟草(Nicotiana amplexicaulis)、阿伦特氏烟草(Nicotiana arentsii)、渐狭叶烟草(Nicotiana attenuata)、贝纳米特氏烟草(Nicotiana benavidesii)、本塞姆氏烟草(Nicotiana benthamiana)、毕基劳氏烟草(Nicotiana bigelovii)、博内里烟草(Nicotiana bonariensis)、洞生烟草(Nicotiana cavicola)、克利夫兰式烟草(Nicotianaclevelandii)、心叶烟草(Nicotiana cordifolia)、伞状烟草(Nicotiana corymbosa)、迪勃纳氏烟草(Nicotiana debneyi)、高烟草(Nicotiana excelsior)、福尔吉特氏烟草(Nicotiana forgetiana)、香烟草(Nicotiana fragrans)、粉蓝烟草(Nicotiana glauca)、粘毛烟草(Nicotiana glutinosa)，古特斯皮德氏烟草(Nicotiana goodspeedii)、哥西氏烟草(Nicotiana gossei)、因古儿巴烟草(Nicotiana ingulba)、川上烟草(Nicotianakawakamii)、奈特氏烟草(Nicotiana knightiana)、蓝格斯多夫烟草(Nicotianalangsdorfi)、狭叶烟草(Nicotiana linearis)、长花烟草(Nicotiana longiflora)、海滨烟草(Nicotiana maritima)、特大管烟草(Nicotiana megalosiphon)、摩西烟草(Nicotiana miersii)、夜花烟草(Nicotiana noctiflora)、裸茎烟草(Nicotiananudicaulis)、欧布特斯烟草(Nicotiana obtusifolia)、西方烟草(Nicotianaoccidentalis)、西方烟草Hesperis亚种(Nicotiana occidentalis subsp.Hesperis)、耳状烟草(Nicotiana otophora)、圆锥烟草(Nicotiana paniculata)、少花烟草(Nicotianapauczjlora)、矮牵牛状烟草(Nicotiana petunioides)、蓝茉莉叶烟草(Nicotianaplumbaginifolia)、四科烟草(Nicotiana quadrivalvis)、雷蒙德氏烟草(Nicotianaraimondii)、残波烟草(Nicotiana repanda)、莲座叶烟草(Nicotiana rosulata)、莲座叶烟草因古儿巴亚种(Nicotiana rosulata subsp.Ingulba)、圆叶烟草(Nicotianarotundifolia)、黄花烟草(Nicotiana rustica)(黄花烟)、赛特氏烟草(Nicotianasetchellii)、拟似烟草(Nicotiana simulans)，茄叶烟草(Nicotiana solanifolia)、斯佩格茨烟草(Nicotiana spegauinii)、斯托克通氏烟草(Nicotiana stocktonii)、香甜烟草(Nicotiana suaveolens)、林烟草(Nicotiana sylvestris)、红花烟草(Nicotianatabacum)、蓝烟草(Nicotiana thyrsiflora)、绒毛烟草(Nicotiana tomentosa)、绒毛状烟草(Nicotiana tomentosifomis)、三角叶烟草(Nicotiana trigonophylla)、荫生烟草(Nicotiana umbratica)、波叶烟草(Nicotiana undulata)、颤毛烟草(Nicotianavelutina)、芹叶烟草(Nicotiana wigandioides)、及烟草属植物的杂交种等。其中，更优选为本塞姆氏烟草、黄花烟草及红花烟草，特别优选为作为烟叶生产的原料而使用的黄花烟草及红花烟草。
除了上述的作用以外，本发明的烟草植物体的一级腋芽的位置偏离叶的根部，因此能够不伤害叶而除去该腋芽，这在实际的栽培实践中是有用的。关于该有用性，优选本发明的烟草植物体在基因组中导入了引起基因的功能抑制的突变，所述基因包含编码了以下多肽的多核苷酸作为编码区域，所述多肽为：
相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽；以及
相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽，
上述功能抑制抑制腋芽的发育。另外，如后述的实施例所示，本发明的烟草植物体特别优选为在NtBl1及NtLS中导入了突变而得到的突变体。
〔2.烟草植物体的制备方法〕
在1个方面，本发明提供一种上述的烟草植物体的制备方法。上述制备方法包括：导入引起基因的功能抑制的突变的步骤，所述基因包含编码了上述的特定的多肽中任一者的多核苷酸作为编码区域。
上述的导入步骤的结果是，通过由该突变引起的上述基因的功能抑制，抑制腋芽的发育。关于通过上述基因的功能抑制来抑制腋芽的发育的概要，如上所述。因此，作为用于实施上述步骤的具体例子，以下列举通过使用了载体的烟草植物体的转化进行的基因的表达抑制及对基因导入突变来进行说明。
作为以基因的表达抑制或向基因导入突变作为目的的烟草植物转化所使用的载体，只要是能够使插入载体内的多核苷酸在植物细胞内表达的载体即可，没有特别限定。作为该载体，例如，优选使用能够借助土壤杆菌将目标多核苷酸导入植物细胞的pBI系、pPZP系及pSMA系的载体等。特别优选为双元载体系(pBIG、pBIN19、pBI101、pBI121、pBI221及pPZP202等)的质粒。
在通过RNAi实现基因的表达抑制的情况下，用于通过RNAi抑制靶基因的表达所使用的触发器(trigger)序列可插入上述载体。该触发器序列例如为：编码具有序列号1、3、5、7、9、11、13、15、17或19所示的氨基酸序列的多肽的多核苷酸(可以有0.1～1％的取代)的一部分的、或者具有序列号2、4、6、8、10、12、14、16、18或20的多核苷酸(可以有0.1～1％的取代)的一部分的、连续至少21～30碱基(例如，21碱基以上、22碱基以上、23碱基以上、24碱基以上、25碱基以上、26碱基以上、27碱基以上、28碱基以上、29碱基以上及30碱基以上)的碱基序列所示的多核苷酸(正义RNA部分)、以及与该多核苷酸互补的碱基序列所示的多核苷酸(反义RNA部分)。上述的“连续至少21～30碱基”的碱基序列更具体是指连续的21碱基以上、23碱基以上、25碱基以上、30碱基以上、35碱基以上、40碱基以上、45碱基以上、50碱基以上、60碱基以上、70碱基以上、80碱基以上、90碱基以上或100碱基以上的碱基序列。
如上所述，本发明的烟草植物体中的基因的表达抑制优选可遗传继承。因此，上述触发器序列优选导入上述烟草植物体的基因组。
对烟草植物体的基因导入突变可以通过公知的基因组编辑技术来实现。作为该基因组编辑技术，可以列举：CRISPR/Cas9system、TALEN及ZFN。在CRISPR/Cas9system中，向导RNA及Cas9蛋白质存在于靶细胞内时，可以进行基因组编辑，在TALEN及ZFN中，融合蛋白质(DNA结合结构域及核酸酶发生了融合)存在于靶细胞内时，可以进行基因组编辑。因此，上述向导RNA及Cas9蛋白质、以及上述融合蛋白质均可以直接导入靶细胞。作为将其直接导入靶细胞的方法，可以列举：PEG法、电穿孔法、以及基因枪(particle bombardment)法等。
在CRISPR/Cas9system中，位于紧邻基因组上的XGG的上游的碱基序列的互补序列与向导RNA的一部分形成碱基对，在该碱基序列内，双链的基因组DNA被Cas9所切断。该碱基序列例如为：编码具有序列号1、3、5、7、9、11、13、15、17或19所示的氨基酸序列的多肽的多核苷酸(可以有0.1～1％的取代)、或者具有序列号2、4、6、8、10、12、14、16、18或20的多核苷酸(可以有0.1～1％的取代)的一部分中的、位于紧邻XGG的上游的连续的10碱基以上(例如，15碱基以上、优选为17碱基以上、更优选为18碱基以上、更进一步优选为19碱基以上、最优选为20碱基以上)。
在TALEN中，形成二聚体的人工核酸酶的一对DNA结合结构域分别与经由5～20碱基的间隔物存在于FokI切断结构域两端的碱基序列结合。该碱基序列存在于双链基因组DNA的一条链及另一条链，因此，上述一对DNA结合结构域的一者与上述一条链结合，另一者与另一条链结合。该DNA结合结构域以33～34氨基酸残基作为重复单元(模块)，由与待结合的碱基数对应数量的模块构成。通过DNA结合结构域结合的碱基序列为：编码具有序列号1、3、5、7、9、11、13、15、17或19所示的氨基酸序列的多肽的多核苷酸(可以有0.1～1％的取代)、或者具有序列号2、4、6、8、10、12、14、16、18或20的多核苷酸(可以有0.1～1％的取代)及形成与该多核苷酸互补的链的多核苷酸的一部分中的、经由5～20碱基的间隔物存在于FokI切断结构域的两端、且各自连续的6碱基以上、优选为10碱基以上、更优选为18碱基以上。
在ZFN中，与TALEN同样，形成二聚体的人工核酸酶的一对DNA结合结构域分别与经由5～20碱基的间隔物存在于FokI切断结构域两端的碱基序列结合。该DNA结合结构域由多个锌指模块(zinc finger module)构成。该碱基序列为：编码具有序列号1、3、5、7、9、11、13、15、17或19所示的氨基酸序列的多肽的多核苷酸(可以有0.1～1％的取代)、或者具有序列号2、4、6、8、10、12、14、16、18或20的多核苷酸(可以有0.1～1％的取代)及形成与该多核苷酸互补的链的多核苷酸的一部分中的、经由5～20碱基的间隔物存在于FokI切断结构域的两端、且各自连续的9碱基以上、优选为12碱基以上、更优选为18碱基以上。
根据所有项目的记载，对于以上的RNAi、CRISPR/Cas9system、TALEN及ZFN的说明，分别可以将具有序列号1、3、5、7、9、11、13、15、17或19所示的氨基酸序列的多肽另外表示成，具有与该多肽90％以上序列同一性、且存在于烟草属所包含的其它物种中的相同分子种类的多肽。另外，同样地，可以将前面段落中记载的具有序列号2、4、6、8、10、12、14、16、18或20的多核苷酸另外表示成，具有与该多核苷酸90％以上序列同一性、且存在于烟草属所包含的其它物种中的同源基因的多核苷酸。
如上所述，导入本发明的烟草植物体的基因突变优选可遗传继承。但是，对于为了基因组编辑而导入烟草植物体的外源性的多核苷酸而言，优选在确认了将希望的突变导入烟草植物体后，从该烟草植物体排除。这是由于，如果上述外源性的多核苷酸保持在烟草植物体中，则因(持续)导入不希望的突变而存在使希望的性状(例如，二级腋芽的抑制)消失、威胁烟草植物体的生存的隐患。
对烟草植物体的基因导入突变可以通过其它生物工程的方法(例如，利用了转座子或土壤杆菌的方法)来实施。该方法的具体例子为将烟草的反转录转座子tnt1或其它植物中的转座子、或者土壤杆菌的T1质粒中的T-DNA导入烟草植物体的方法。
或者，对烟草植物体的基因导入突变可以通过其它方法(烟草植物体的突变原处理)来实施。作为突变源的例子，可以列举小分子的化合物(例如，甲磺酸乙酯(EMS)、N-乙基-N-亚硝基脲(ENU)、叠氮化钠等)、以及放射线(例如，γ射线、重离子束、X射线、中子射线、紫外线等)。
突变可以导入能够再生的任意烟草植物体。该烟草植物体的例子为种子、根、叶、花、生殖器官或胚，优选为种子。
通过以上方法得到的可以是具有各种突变的(或者不具有突变的)植物体的突变体群。因此，可以从该突变体群中进一步挑选显示出希望的表型的个体。作为挑选个体的例子，对于从在使用突变原进行了处理的情况下得到的突变体群(实验对象集合)中挑选希望的个体的步骤进行说明。
在T基因组、S基因组两者总计4个基因中具有突变的功能缺失的烟草突变体例如可以通过以下方法获得。如上所述利用突变原对烟草进行处理，制备在全部烟草基因组中发生了突变的烟草的突变体群(实验对象集合)，提取基因组DNA。利用S基因组、T基因组各自的基因特异性引物，由实验对象集合的基因组DNA扩增靶基因(多核苷酸)，确定其产物的碱基序列，挑选具有纯合型突变的品系。首先，获得在S基因组、T基因组分别具有纯合型突变的品系(M2)，制备将它们交配而成的F1。进而，培育其自身繁殖后代(F2)，从其中获得S、T两个基因组均具有纯合型突变的品系(由于双因子隐性，可以以1/16的概率获得)。
本发明的烟草植物体的制备方法进一步包括如下步骤：从在上述的生成步骤中生成的烟草植物体中，挑选除去一级腋芽后发育的二级腋芽的数量或重量与上述野生株植物相比减少为1/2以下的个体。上述挑选的步骤例如可以基于编码上述的特定多肽的基因的破坏、突变或表达抑制来实施。
基因的突变或破坏可以通过检测该基因中有无突变来确定。作为检测基因中的突变的方法，只要是能够判断有无突变的方法即可，可以是以下方法：(1)利用市售的测序仪等直接解读DNA碱基序列的方法；(2)使用SSCP(Single StrandConformationPolymorphism)法根据电泳距离的不同检测序列中的不同的方法；(3)使用CycleavePCR法检测SNP(Single Nucleotide Polymorphism)的方法；(4)通过使用T7EndonucleaseI等切断错配部位而检测有无突变的方法；(5)根据利用制限酶处理有无切断可以判断有无突变的CAPS(Cleaved Amplified Polymorphic Sequence)法；(6)通过使用有意包含错配的引物组，从而根据有无利用制限酶进行的切断来判断有无突变的dCAPS(derived CAPS)法；(7)通过使用对突变序列特异性杂交的探针检测探针是否杂交，从而判断有无突变的方法(使用了TaqMan探针的PCR法、MassARRAY分析法)；(8)在缺失、插入的情况下，根据电泳的移动程度的差别来检测突变的方法等。或者，基因的突变或破坏可以通过作为基因的改变结果而产生的蛋白质或野生型蛋白质的表达量的检测(例如，蛋白质印迹法(western blotting))来确定。
在上述导入突变的步骤之前，为了确定待抑制表达的基因和/或待导入突变的基因，可以根据需要实施以下所示的步骤(1及2)。
1.推测控制腋芽的发育的烟草基因的分离
可能控制腋芽的基因可以从其它植物的现有技术文献(例如，确认了基因与腋芽的关系的非专利文献)中选择，以该基因的碱基序列及氨基酸序列的同一性作为指标，从烟草中获得基因。例如，通过对公知的数据库中收录的序列进行基于blast(Basic LocalAlignment Search Tool)的检索，可以获得公知的烟草基因、或者与烟草亲缘关系接近的植物种的基因(例如，番茄)的碱基序列、氨基酸序列。在公知的序列为部分长度的情况下，可以利用已知的序列信息，通过通常的方法、例如通过Race(Rapid amplification ofcDNA ends)法从cDNA文库中筛选，获得全长cDNA。
新的能够控制腋芽的基因例如可以通过挑选由作为目标的组织、处理所表达的基因而获得。作为目标的组织、处理可以基于以下举出的信息来选择。已知，与腋芽的原基形成相关的基因在腋芽原基形成之前表达，与腋芽原基的保持、生长相关的基因在腋芽原基中表达(例如，LS、Blind基因)。已知与腋芽的休眠、发育相关的基因在休眠、脱离休眠的腋芽中表达增加减少(例如BRANCHED1)。另外，已知若干植物激素与腋芽的控制相关。生长素与顶芽优势相关，独脚金内酯与腋芽的发育抑制相关，细胞分裂素与腋芽的伸长相关，脱落酸与休眠相关。
新挑选能够控制腋芽的发育的基因可以通过利用了表达特异性的通常的方法来实施。作为该方法，可以列举例如：(1)从cDNA的碱基序列获得基因表达分析数据的方法、以及制作在对象组织中表达的基因的cDNA文库并对末端序列进行测序的方法、将限制酶片段串联并进行测序的SAGE(Serial Analysis of Gene Expression)法等。(2)通过差示杂交获得基因表达分析数据的方法。已知有微阵列(マクロアレイ)、DNA芯片。(3)通过差别显示法可以获得在多个样品之间表达水平不同的基因(Differentially Expressed Genes：DEGs)。可以举出比较PCR扩增片段的量的方法。
分离的基因的扩增
多核苷酸的扩增可以通过PCR(Polymerase Chain Reaction)法进行，也可以通过LCR(Ligase Chain Reaction)法、LAMP(Loop-Mediated Isothermal Amplification)法等进行。
用于扩增多核苷酸的引物只要是能够从S基因组、T基因组的基因混合存在的烟草基因组中特异性地扩增各基因组的目标基因的引物即可。只要能够特异性地扩增目标基因，也可以包含1个或1个以上的取代、缺失、插入、添加。另外，引物可以根据需要用荧光物质、放射线等进行标记。
作为扩增的模板而使用的基因组DNA的提取可以通过公知的方法来进行，可以使用市售的提取试剂盒。可以是基因组DNA经过简易提取而得到的粗纯化物，也可以是经过纯化工序得到的纯化物。
2.推测与腋芽的发育相关的基因的鉴定
制备作为对象的抑制了基因的表达、功能的重组体及突变体，通过在温室、人工气候室、大棚或田地中进行栽培，可以对基因的效果进行确认。通过将产生的腋芽的数量、重量与对照进行比较，可以确认对腋芽的产生、发育的效果。对于腋芽的数量、重量而言，可以不进行打顶，也可以进行打顶，优选通过打顶使其脱离休眠而促进腋芽的发育来进行。腋芽的数量、重量的调查可以在任意一个时期进行1次，也可以进行多次。1次测量中可以对一级腋芽进行评价，但不适于对二级腋芽、三级腋芽的评价，因此优选进行多次。为了确认对于二级腋芽、三级腋芽的效果，优选分别除去一级腋芽、二级腋芽。一级腋芽、二级腋芽的除去可以在各腋芽发育后的任意时间进行，但必须做到没有剩余。优选在确认了腋芽的茎的伸长的时期除去。在多次调查的情况下，优选设置间隔，分为一级腋芽、二级腋芽、三级腋芽进行调查。例如，可以以如下方法进行：每周1次分别对以一级腋芽、二级腋芽、三级腋芽作为对象的腋芽数量进行计数，对其进行采集，并调查重量。
调查可以仅着眼于特定的腋芽(例如二级芽)来进行，也可以仅集中于数量、重量来分别进行。在该情况下，优选以次数、间隔分别适合调查的方法来进行。
〔3.选择性地抑制烟草植物体中的二级腋芽的方法〕
在一个方面，本发明提供一种选择性地抑制烟草植物体中的二级腋芽的方法。二级腋芽的选择性抑制可以通过导入引起烟草植物体中的基因的功能抑制的突变来进行，所述基因包含编码上述特定多肽中任一种的多核苷酸作为编码区域。这里，该功能抑制是抑制腋芽的发育。即，本发明的一个方面的烟草植物体发生了该功能抑制，本发明的另一方面的烟草植物体的制备方法在其制备过程中在烟草植物体中发生该功能抑制。因此，对于在烟草植物体中发生该功能抑制的方法的详细情况，可以参照与烟草植物体及其制备方法有关的前面的项目。
对以上各实施方式进行总结，本发明可以概括如下。
即，一种烟草植物体，其在基因组中导入了引起基因的功能抑制的突变，所述基因包含编码了以下(1)～(5)的多肽中任一者的多核苷酸作为编码区域，其中，
所述功能抑制抑制腋芽的发育，
(1)～(5)的多肽为：
(1)相对于序列号1所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号3所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(2)相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(3)相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(4)相对于序列号13所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号15所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；以及
(5)相对于序列号17所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号19所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者。
在上述烟草植物体中，优选上述功能抑制抑制上述腋芽中在除去一级腋芽后发育的二级腋芽的发育。
在上述烟草植物体中，优选上述功能抑制使上述二级腋芽的数量或重量与野生株植物相比减少至1/2以下。
在上述烟草植物体中，优选上述功能抑制为与野生株植物相比时上述多肽的存在量减少。
在上述烟草植物体中，优选上述功能抑制为与野生株植物相比时上述多肽的翻译量减少。
在上述烟草植物体中，优选上述功能抑制为与野生株植物相比时来自上述基因的mRNA的转录量减少。
在上述烟草植物体中，优选上述功能抑制为从上述基因转录而成的mRNA的分解的促进。
在上述植物体中，优选在上述基因中导入了上述突变。
在上述烟草植物体中，优选通过自然突变、突变原处理、基因组编辑或基因敲除导入了上述突变。
在上述烟草植物体中，优选上述突变为表达促进上述mRNA分解的因子的多核苷酸插入上述基因存在的区域以外。
在上述烟草植物体中，优选上述因子为反义RNA分子、RNAi分子或共抑制分子。
上述烟草植物体优选属于红花烟草或黄花烟草。
一种烟草植物体的制备方法，该方法包括导入引起基因的功能抑制的突变的步骤，所述基因包含编码了以下(1)～(5)的多肽中任一者的多核苷酸作为编码区域，其中，
上述功能抑制抑制腋芽的发育，
(1)～(5)的多肽为：
(1)相对于序列号1所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号3所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(2)相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(3)相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(4)相对于序列号13所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号15所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；以及
(5)相对于序列号17所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号19所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者。
在上述制备方法中，优选进一步包括从上述导入的步骤中生成的个体中挑选上述腋芽中在除去一级腋芽后发育的二级腋芽的发育受到抑制的个体的步骤。
在上述制备方法中，优选在上述挑选的步骤中，挑选上述二级腋芽的数量或重量比野生株植物有所减少的个体。
在上述制备方法中，优选上述导入的步骤包括将上述突变导入上述基因。
在上述制备方法中，优选上述导入的步骤通过自然突变、突变原处理、基因组编辑或基因敲除来实施。
在上述制备方法中，优选上述导入的步骤包括将表达促进从上述基因转录而成的mRNA的分解的因子的多核苷酸插入上述基因存在的区域以外。
在上述制备方法中，优选上述因子为反义RNA分子、RNAi分子或共抑制分子。
上述烟草植物体或通过制备方法得到的烟草植物体的后代，或者通过该烟草植物体的交配而得到的育种后代。
从上述烟草植物体、通过上述制备方法得到的烟草植物体、或者上述后代或育种后代收获得到的烟叶。
以下，示出实施例对本发明的实施方式进行详细说明。当然，本发明并不限定于以下的实施例，对于细节部分可以有各种方式。另外，本发明并不限定于上述的实施方式，可以在权力要求所示的范围进行各种变更，将各公开的技术方式适当组合而得到的实施方式也包含于本发明的技术范围。另外，参考并援引本说明书中记载的全部文献。
实施例
〔1.烟草植物中的与腋芽发育相关的候选基因〕
通过Basic Local Alignment Search Tool(blast)分析确定了其它植物中的与腋芽发育相关的多个基因(拟南芥(Arabidopsis thaliana)的Revolutla(REV)、番茄的Lateral suppressor(LS)及番茄的Blind(Bl))的烟草直向同源的候选(以下，简称为候选组A)。另一方面，通过转录组分析确定了烟草植物中与腋芽发育相关的基因的候选(以下，简称为候选组B)。以下，对于各分析及基于分析的结果而得到的基因进行说明。
(1-1.候选组A)
(a)blast分析
以拟南芥的REV基因的氨基酸序列作为查询，在NCBI的网页(http://blast.ncbi.nlm.nih.gov/Blast.cgi)进行tblastn，结果是得到了具有80％的高氨基酸序列同一性的番茄的REV同源基因序列。以番茄的REV同源基因的氨基酸序列作为查询，对cDNA文库(来自于Tsukuba(つくば)1号的叶、茎顶端及根的混合物)的EST(ExpressedSequence Tag)分析结果进行tblastn，挑选了烟草的推测REV cDNA克隆组。
与番茄的LS基因具有87％的氨基酸序列同一性的烟草的cDNA序列收录于公开的DB(收录号：EU935581)。另外，与EU935581具有高同一性的烟草EST序列(收录号：AM848584)收录于公开的DB。
以番茄的Bl基因的氨基酸序列作为查询，对cDNA文库(来自于Tsukuba1号的叶、茎顶端及根的混合物)的EST分析结果进行tblastn，挑选了烟草的推测的Bl克隆组。
(b)来自于个体的基因组DNA片段及cDNA(来自于total RNA)的制备
按照简易提取法或CTAB法从烟草(Tsukuba 1号或Petit Havana SR-1(SR-1))的叶中提取了基因组DNA片段。CTAB法是公知的方法，因此省略其详细说明。按照以下步骤进行了简易提取法。使用破碎装置(多珠振荡器(multi-bead shocker)(安井器械)或ShakeMaster Neo(Biomedical Science)等)将放入0.3～0.5ml的提取缓冲液(0.2M的Tris-HClpH8.0、0.4M的NaCl、25mM的EDTA、0.5％的SDS)中的叶片磨碎(2500rpm、1分钟)。从磨碎后的悬浮物中取得上清，通过乙醇沉淀，从上清中纯化了基因组DNA片段。
如下所述提取了total RNA。将烟草的茎顶端、苗及腋芽分别浸渍于RNAlater(Ambion)，进行冷冻保存。然后，使样品融化，在其中添加0.5ml的RTL缓冲液(QIAGEN)，所述RTL缓冲液中加入了20μl的1M DTT。使用多珠振荡器(安井器械)将混合物磨碎(2500rpm、1分钟)。通过对磨碎后的悬浮物进行离心分离(15000rpm、10分钟)而得到上清，使用Magtration(Precision System Science)或RNeasy Kit(QIAGEN)在Dnase存在的条件下从该上清中纯化了total RNA。
使用以下任一种试剂盒，按照各试剂盒中附带的手册从total RNA中纯化了cDNA。
·PrimeScript II 1st strand cDNA Synthesis Kit(Takara Bio)
·PrimeScript RT reagent kit with gDNA Eraser(Takara Bio)。
(c)候选组A的基因的生成
通过使用在(b)中得到的cDNA作为模板的RT-PCR对3个基因进行了扩增。在使用了PrimeSTAR Max DNA Polymerase(Takara Bio)作为酶的情况下，如下所述设定了反应条件。
94℃下30秒钟
以98℃下10秒钟、55℃下5秒钟及72℃下10秒钟＊作为1个循环，30～40个循环
72℃下10秒钟
＊72℃的延伸反应设定为扩增片段的每1kb长度10秒钟。
在使用了Tks Gflex DNA Polymerase(Takara Bio)作为酶的情况下，如下所述设定了反应条件。
94℃下30秒钟
以98℃下10秒钟、55℃下15秒钟、68℃下60秒钟＊作为1个循环，30～40个循环
68℃下60秒钟
＊68℃的延伸反应设定为扩增片段的每1kb长度60秒钟。
将RT-PCR用的对象基因及引物的组合示于以下。
(第1组：NtLS、T基因组、Tsukuba 1号的苗)
LS_Tom_F1:AGGTTCTTCTTCCTTAATATTGAGTC、及NtLS_qRV1:ATCTAAGGCCTAAAGAGTGAGCAAAT的组合
LS2_F2:ACACCTAATGCATCATCTAATGTT、及LS_Syl_R1:CAAATAAAGATTAAGTTCAGGATCTG的组合
(第2组：NtLS、S基因组、Tsukuba 1号的苗)
LS_F2_seq:ATTTCCCCTCCTCCATCATTG、及NtLS_qRV1:ATCTAAGGCCTAAAGAGTGAGCAAAT的组合
LS1_F2:CTTGACACCATCTAATGTTGTTG、及LS_Tom_R1:CAAATAAAGATTAAGTTCAGGATCTG的组合
(第3组：NtREV、T基因组、Tsukuba 1号的苗)
REV_RT_F2:AAGCTGTTTGCAGGGAATATATC、及G053330_RV3:TCTCTGGCTAAATGTTCGAAG的组合
REV_RT_F3:GTAAGTTGTGAGTCTGTGGTAACTAC、及REV_RT_R1:GGAAACAAACATCTGCACTCAA的组合
(第4组：NtBl、S基因组、Tsukuba 1号的苗)
Bl1_F1seq2:GTCCATCTGTCTATATAGGTAGAATG、及Bl1-2_RT_R1:TGAATCTTCTTGGCAACCCCC的组合
(第5组：NtREV、S基因组、SR-1的腋芽)
Ns_in0_F1:TTGTTTGGGATTTTGGGGTTTGAGGG、及REV_S_R1:AATTGTATGGCCAAGTGGCATTATTATCTGA
REV_S_F1:CACTTCCGTTCCTCTTTCACCGCTG、及NtREV_S_RV1:TCCGTTCAACTGTGTTCCTGG
(第6组：NtREV、T基因组、SR-1的腋芽)
REV_RT_F2:AAGCTGTTTGCAGGGAATATATC、及NtREV1_RV1:TCCGTTCAACTGTGTTCCTG
(第7组：NtLS、S基因组、SR-1的腋芽)
LS_Tom_F1:AGGTTCTTCTTCCTTAATATTGAGTC、及LS_Tom_R1:CAAATAAAGATTAAGTTCAGGATCTG的组合
(第8组：NtLS、T基因组、SR-1的腋芽)
LS_Tom_F1:AGGTTCTTCTTCCTTAATATTGAGTC、及LS2-F2compR:AACATTAGATGATGCATTAGGTGT的组合
LS2-F2:ACACCTAATGCATCATCTAATGTT、及LS_Syl_R1:TTGGCCTCTAATTAAATAGACTGATA的组合。
另外，通过使用了在(b)中得到的基因组DNA片段作为模板的基因组PCR对3个基因进行了扩增。使用的酶及酶所对应的反应条件与上述的RT-PCR相同，因此将对象基因及引物的组合示于以下。
(第1组：NtREV、S基因组、Tsukuba 1号的叶)
REV_F3:TCTCAAAGCTGGCTGTTTTATGTAT、及REV_R14:TACCATTCTCCAGGGTGGTTGTGTAT的组合
Ns_in4_F1:GAAAATTCAGTATTGCCATGTC、及G053330_RV2:GCAAAAACTAGTTCAGAACA的组合
NtREV_TrFW2:CACCGCCTATGTAGCTTCGTCAATG、及
NtREV_RT-R1:GGAAACAAACATCTGCACTCAA的组合
(第2组：NtREV、T基因组、Tsukuba 1号的叶)
REV_F3:TCTCAAAGCTGGCTGTTTTATGTAT、及REV_R14:TACCATTCTCCAGGGTGGTTGTGTAT的组合
Nt_in4_F1:AAAAAAATTCAGTATTGCCACGTGC、及G053330_RV2:GCAAAAACTAGTTCAGAACA的组合
NtREV_TrFW2:CACCGCCTATGTAGCTTCGTCAATG、及NtREV_RT-R1:GGAAACAAACATCTGCACTCAA的组合
(第3组：NtLS、S基因组、Tsukuba 1号的叶)
LS_F1_seq:AGGTTCTTCTTCCTTAATATTGAGTC、及LS_TRV_R3:TCGCTTGATTAGCAGTCAGC的组合
LS_F1_seq:AGGTTCTTCTTCCTTAATATTGAGTC、及NtLS_QPCR_RV1:ATCTAAGGCCTAAAGAGTGAGCAAAT
LS_TRV_F3:CACCGAAGAAACTGATGATCAACGG、及LS_TRV_R2:GAAGACCTCTTTGTCCTTCACCATGCAG的组合
(第4组：NtLS、T基因组、Tsukuba 1号的叶)
LS_F2_seq:ATTTCCCCTCCTCCATCATTG、及NtLS_QPCR_RV1:ATCTAAGGCCTAAAGAGTGAGCAAAT的组合
LS_TRV_F3:CACCGAAGAAACTGATGATCAACGG、及LS_TRV_R2:GAAGACCTCTTTGTCCTTCACCATGCAG的组合
(第5组：NtBl1、S基因组、Tsukuba 1号及SR-1的叶)
Bl1_F1seq2:GTCCATCTGTCTATATAGGTAGAATG、及Bl1_R1seq:CACCATGTTTGATATTAGGCCTTA的组合
Bl1_F3seq2:TGATGAGATTTATGTTGGGAACTG、及Bl1_R2seq:TCTCATCATTGAACACGAACATACT的组合
(第6组：NtBl1、T基因组、Tsukuba 1号及SR-1的叶)
Bl1_F1seq1:CCACTTGTCTATATAGCAAGAAAGA、及Bl1_R1seq:CACCATGTTTGATATTAGGCCTTA的组合
Bl1_F2seq:CTAAGGCCTAATATCAAACATGGT、及Bl1_R2seq:TCTCATCATTGAACACGAACATACT的组合。
(d)得到的基因的序列确定
使用Zero Blunt TOPO PCR Cloning Kit for Sequencing Kit(LifeTechnology)对将上述3个基因扩增而得到的PCR产物分别进行克隆。需要说明的是，通过将琼脂糖凝胶电泳及MiniElute柱(QIAGEN)组合的通常方法，根据需要在克隆前对该PCR产物进行了纯化。使用BigDye(注册商标)Terminator v3.1 Cycle Sequencing Kit(ABI)在毛细管测序仪3730xl DNA Analyzer(ABI)长确定了克隆得到的基因的各碱基序列(序列号21～30)。
(1-2.候选组B)
为了对预想在植物体的叶原基中表达提高的基因进行鉴定，如下所述进行了转录组分析。
(a)RNA提取样品的制备
制作了包埋有从播种起4～5周后的烟草苗(品种：SR-1)中得到的茎顶端部的石蜡块(具体参照Takahashi H,Kamakura H,Sato Y,Shiono K,Abiko T,Tsutsumi N,NagamuraY,Nishizawa NK,Nakazono M.(2010)A method for obtaining high quality RNA fromparaffin sections of plant tissues by laser microdissection.J Plant Res 123:807-813)。使用切片机(RM2125RTS；Leica)将该石蜡块制成厚度20μm的连续切片。从该连续切片中选择包含了Shoot ApicalMeristem(SAM)的中心部及其附近的切片。使用AppliedBiosystems(注册商标)ArcturusXT(商标)激光捕获显微切割系统，从该切片中分别将未形成腋芽原基的叶原基的根部(腋芽原基样品)、叶原基及茎顶端分生组织的下部(对照样品)切成直径100～200μm的大小。将腋芽原基样品及对照样品分别回收于CapSure(注册商标)LCM Cap(Applied Biosystems)，转移至RNA提取用的管中，直至RNA提取为止，在-80℃下冷冻保存。
(b)RNA纯化
使用PicoPure RNA isolation Kit(Arcturus)，根据试剂盒附带的手册从(a)的RNA提取样品中纯化了total RNA。使用生物分析仪(Agilent Technologies)推测纯化的RNA溶液的RNA浓度，并确认了品质(RNA的分解程度)。
(c)使用了下一代测序仪(454Genome Sequnecer Titanium、Roche)的碱基序列确定及基因表达量的预测
将在(b)中得到的RNA送至Takara Bio，委托该公司从该RNA制作用于测序仪分析的cDNA文库。将该cDNA文库中含有的cDNA的5’末端部分的碱基序列分析委托给Genaris公司。在碱基序列的确定中，将来自于cDNA文库的植物的每个部分的3/4板的部分进行测序。通过使用了由各部分得到的全部序列信息的从头组装(de novo assembly)分析，得到了组装序列。将从来自于各部分的cDNA文库得到的前导序列与得到的组装序列进行匹配(比对)，对于各部分统计与各基因对应的读取数(リード数)。将统计的读取数在来自于各部分的cDNA文库之间进行标准化，并且基于该读取数对各部分预测了全部基因的表达量。
(d)候选组B的确定
在腋芽原基样品(参照上述的(a))中有200碱基以上的序列数据，选择了腋芽原基样品的读取数为4以上且具有与对照样品相比为10倍以上的表达量的基因作为与腋芽原基形成相关的1次候选基因。可以推测，形成腋芽原基的这样的成为控制器官形成的主开关的基因是控制多个基因表达的转录因子。因此，从根据1次表达特性选择的候选基因中进一步集中于变码转录因子的可能性高的基因，由此挑选了2次候选基因。对这些基因的表达抑制是否抑制烟草中的腋芽的发育进行确认，最终确定了候选组B(2个基因)。
(e)2个基因的全长序列及cDNA序列的生成
根据下一代测序分析的结果，通过组装前导序列得到了共有序列“isogroup15360”及“isogroup07437”。通过使用了这些共有序列的Race、RT-PCR及基因组PCR，生成了上述2个基因的全长序列及cDNA序列。
使用按照1-1.(b)的记载制备的total RNA，并且使用SMARTer RACE cDNAAmplification Kit(Clontech)，按照试剂盒中附带的手册实施了Race。Race的nested PCR中使用了稀释300倍的1st PCR产物作为模板。在Race中如下所述设定了反应条件。
(1st PCR)
以98℃下10秒钟及72℃下10秒钟作为1个循环，5个循环
以98℃下10秒钟、70℃下5秒钟及72℃下5秒钟作为1个循环，5个循环
以98℃下10秒钟、60℃下5秒钟及72℃下5秒钟作为1个循环，25个循环
(nested PCR)
以98℃下10秒钟、55℃下5秒钟及72℃下5秒钟作为1个循环，25个循环。
作为Race用的引物，使用了试剂盒附带的引物、以及对以下所示的基因的特异性引物。
(1st PCR：#15360、S基因组及T基因组、SR-1的茎顶端)
5’Race1st引物：R-GAACCACCAGGGACTAAACTCTGCAA、及3’Race1st引物：F-TTGCAGAGTTTAGTCCCTGGTGGTTC的组合
(nested PCR：#15360、S基因组及T基因组、SR-1的茎顶端)
5’RaceNested引物：R-GAAACGATCACTGATTCTATGCC、及3’RaceNested引物：F-TACAATGTTAGAAGAAGCAATTCAC的组合。
如1-1.(c)所述进行了RT-PCR。使用的引物及对象基因如下所述。
(第1组：#07437、S基因组、SR-1的茎顶端)
正向：TACTTCCCTTTCTCACTTTGGTTTC、及反向：AATATTCCCATCAATAGATCACAAC的组合
(第2组：#07437、T基因组、Tsukuba 1号的苗)
07437_T_F1:CTACTACATCACTTAATATCATTCATT、及07437_Tom_RT_R1:CAATAGATTGCAACTTTACATTAGTCG的组合
(第3组：#07437、S基因组、Tsukuba 1号的苗)
07437_S_F1:TACTATCACTTAATACCATCATTCATC、及07437_Syl_RT_R1:CCCATCAATAGATCACAACTTTAGT的组合
(第4组：#15360、S基因组、Tsukuba 1号的苗)
15360-2_F2:AAATAGAGGTAATTAGTTGTATCAATGG、及15360-Nts_R2:ACAACATACCATACTACCACACACTA的组合
(第5组：#15360、T基因组、Tsukuba 1号的苗)
15360-1_F1:TGCATGGACAATCTCCTCTT、及15360-Nts_R2:ACAACATACCATACTACCACACACTA的组合
(第6组：#15360、S基因组、SR-1的腋芽)
15360-2_F1:GCATGGACAATCTCATCTTCTC、及15360-1_R1-2:CAACAGGAGTTGAGTTATTCTCAT的组合
(第7组：#15360、T基因组、SR-1的腋芽)
15360_TrFW1:CACCTTCTTCAAGCAAAATTAATGAC、及15360_TrRV1:ATTAGAGTCATGAGCCATTAGC的组合。
如1-1.(c)的记载所述进行了基因组PCR。使用的引物及对象基因如下所述。
(第1组：#15360、S基因组、Tsukuba 1号的叶)
15360-2_F1:GCATGGACAATCTCATCTTCTC、及15360-2_R1:CTGGGCAATATTCCACCATT的组合
15360-2_F2:AATGGTGGAATATTGCCCAG、及15360_NtsR2:ACAACATACCATACTACCACACACTA的组合
(第2组：#15360、T基因组、Tsukuba 1号的叶)
15360-1_F1:TGCATGGACAATCTCCTCTT、及15360-1_R1-2:CAACAGGAGTTGAGTTATTCTCAT的组合
15360-1_F2-2:ATGAGAATAACTCAACTCCTGTTG、及15360_NtsR2:ACAACATACCATACTACCACACACTA的组合
(第3组：#07437、S基因组、Tsukuba 1号的叶)
07437-S_F1:TACTATCACTTAATACCATCATTCATC、及07437-S_R1:TCCCTGTACTTTGGGACATGA的组合
07437-S_F2:GTGTACCAGCTAGTTATTATTGCG、及07437-S_R2:CCTGATCCGTTCTGATAGATCG的组合
07427-S_F3:ATTTGTTAAAAAGTTGTAATAAAATTGG、及07437-S_R3:TTTCTTTGAATTGCTAACGAGGA的组合
07437-S_F4:TCCTCGTTAGCAATTCAAAGAAA、及07437-S_R5:AGAATATAAAGAGCAGCCTGAATTAC的组合
07437-S_F1:TACTATCACTTAATACCATCATTCATC、及07437-S_R2:CCTGATCCGTTCTGATAGATCG的组合
07437-S_F2:GTGTACCAGCTAGTTATTATTGCG、及07437-S_R3:TTTCTTTGAATTGCTAACGAGGA的组合
(第4组：#07437、T基因组、Tsukuba 1号的叶)
07437-T_F1:CTACTACATCACTTAATATCATTCATT、及07437-T_R1:TCCCTGTACTTTGGGACATGA的组合
07437-T_F2:TGCATTAACATGAATGCGAC、及07437-T_R2:TCTAAATAGCGAGTAATAAGGATGAGA的组合
07437-T_F3:GTTTGTTAAAAAATTGTAATAAACTTGG、及07437-T_R3:TTTCTTTGAAGTGCAAAAGGAAT的组合
07437-T_F4:ATTCCTTTTGCACTTCAAAGAAA、及07437-T_R4:ATTATGGAAAAACAACTCTTCTATT的组合
07437-T_F1:CTACTACATCACTTAATATCATTCATT、及07437-T_R2:TCTAAATAGCGAGTAATAAGGATGAGA的组合
07437-T_F2:TGCATTAACATGAATGCGAC、及07437-T_R3:TTTCTTTGAAGTGCAAAAGGAAT的组合。
(1-3.基因组上的目标基因的全长序列确定)
按照1-1.(b)中记载得到了基因组DNA片段。通过使用了以该基因组DNA片段作为模板的PCR，使目标基因的5’上游及3’下游分别扩增。PCR的反应条件如1-1.(c)中的记载所述。PCR中使用的引物如下所述。
[表1]
使用Zero Blunt(注册商标)TOPO(注册商标)PCR Cloning Kit(Thermo FisherScientific)，按照其手册所述，利用扩增后的PCR产物分别对大肠杆菌(Mach1(商标)-T1R)进行了转化。在板上接种转化后的大肠杆菌。使用在板上形成的菌落，进行菌落PCR。使用ExoSAP-IT(注册商标)For PCR Product Clean-UP(Affimetrix)按照其手册对菌落PCR的扩增产物进行了纯化，然后用于后述的测序反应的模板。同时，在对菌落进行液体培养后，使用QIAGEN Plasmid Mini Kit(QIAGEN)从培养的菌体中提取出质粒。提取出的质粒也用于后述的测序反应的模板。
使用BigDye(注册商标)Terminator v3.1 Cycle Sequencing Kit(AppliedBiosystems)如其手册所述使上述模板进行反应。使用BigDye(注册商标)XTerminator(商标)Purification Kit(Thermo Fisher Scientific)对反应生成物进行纯化。通过毛细管测序仪3730xl DNA Analyzer(Applied Biosystems)确定了纯化后的反应生成物的碱基序列。测序引物根据序列信息适当设计而使用。
通过使用ATGC测序序列组装软件(Genetics)连接确定的碱基序列，确定了目标基因中的非翻译区域的碱基序列。通过进一步连接非翻译区域及结构基因部分，确定了目标基因的完全长度的基因组DNA序列(序列号54～63)。
(1-4.结果)
将确定为候选组A的REV、LS及Bl的烟草直向同源基因分别命名为NtREV、NtLS及NtBl1。另外，根据转录组分析的结果，将确定为候选组B的基因分别命名为#15360及#07437。
〔2.候选物A及B中任一者的表达抑制对于腋芽发育的影响的确认〕
为了研究候选物A及B中任一者的表达抑制对于腋芽发育的影响，对抑制了各基因的表达的重组体(以下，简称为重组体)中腋芽发育的变化进行了确认。
(2-1.重组体的制备)
(a)转化的准备
为了制备重组体，首先，如下所述准备了转化用的载体。
以基于1.的结果的生成的来自于SR-1的cDNA为模板，使用PrimeSTAR Max DNAPolymerase(Takara Bio)通过PCR对用于抑制NtREV、NtBl1、NtLS、#15360及#07437(也统称为对象基因)的表达的RNAi触发器序列进行了扩增。PCR的条件及引物如下所述。
(PCR条件)
94℃下30秒钟
以98℃下10秒钟、55℃下5秒钟、及72℃下10秒钟作为1个循环，30～40循环
72℃下10秒钟
(#15360用引物)
15360_TrFW1:CACCTTCTTCAAGCAAAATTAATGAC、及
15360_TrRV1:ATTAGAGTCATGAGCCATTAGC的组合
(#07437用引物)
07437_TrFW1:ACCACCTGGTTTTAGGTTTCATCC、及
07437_TrRV1:TATTCTGCATATCACCCATTCC的组合
(NtLs用引物)
LS_TRV_F3:CACCGAAGAAACTGATGATCAACGG、及
LS_TRV_R3:TCGCTTGATTAGCAGTCAGC的组合
(NtBl1用引物)
N.t_BL(hit1)_TRV_F1:CACCTCAAGAAAAAGCTTATGGG、及
N.t_BL(hit1)_TRV_R1:GCAGCAGCTAACAAGTTGTA的组合
(NtREV用引物)
NtREV_TrFW2:CACCGCCTATGTAGCTTCGTCAATG、及
NtREV_TrRV2:CACTGTAGCCAGAGACCACA的组合。
如上所示，为了NtREV的表达抑制，选自位于HD-Zip结构域的下游侧(3’侧)的翻译区域的序列作为RNAi触发器序列，为了NtBl1的表达抑制，选自位于Myb结构域的下游侧(3’侧)的翻译区域的序列作为RNAi触发器序列，为了NtLS的表达抑制，选择翻译区域的5’末端侧作为RNAi触发器序列，为了#15360的表达抑制，选择包含了bHLH结构域的序列作为RNAi触发器序列，为了#07437的表达抑制，选择NAM结构域区域的序列作为RNAi触发器序列。另外，通过PCR扩增的各RNAi触发器序列设计为在5’末端添加CCAC、且RNAi触发器序列为400～500bp的长度。
将PCR产物克隆于pENTR(商标)/D-TOPO载体(Life Technology)，在确认了各RNAi触发器序列的碱基序列后，使用Gateway LR Clonase II Enzyme Mix(Life Technology)将各RNAi触发器序列导入了pSP231载体。为了确认导入的序列，将导入pSP231载体(是在pHellsgate12(参照文献：Wesley et al.,2001,Plant J.,27,581-590)的SacI部位插入了GFP(Green-fluorescent protein基因)表达盒而得到的载体，是触发器序列的倒位重复序列包夹着pdk/cat内含子而配置、且能够通过花椰菜花叶病毒35SRNA基因启动子表达RNAi序列的双元载体)的各RNAi触发器序列单独通过PCR使用TaKaRa Ex Taq及PrimeSTAR MaxDNA Polymerase(Takara Bio)对正义链及反义链分别进行扩增。在使用MiniElute(QIAGEN)对PCR产物进行了纯化后，进行了测序。导入pSP231载体的RNAi触发器序列的碱基序列如序列号31(NtREV)、序列号32(NtBl1)、序列号33(NtLS)、序列号34(#15360)及序列号35(#07437)所示。需要说明的是，在序列表所示的碱基序列中，省略了位于5’末端的CACC。
使用包含了各触发器序列的pSP231载体通过电穿孔对土壤杆菌(Agrobacteriumutumefaciens)LBA4404进行转化。在LBA4404中，通过PCR确认了各RNAi触发器序列被扩增后，将该土壤杆菌用于烟草的转化。
(b)烟草的转化、及转化后的种子的采种
使用品种MC1(NtBl1的转化)或SR-1(NtREV、NtLS、#15360及#07437各自的转化)通过如下所述的通常方法进行了烟草的转化。使转化后的上述土壤杆菌感染烟草叶的切片，在包含了卡那霉素的Linsmaier and Skoog培养基中进行培养，由此从得到的愈伤组织得到了卡那霉素耐性的再分化个体。从这些再分化个体中挑选了确认到叶整体中基于GFP的强荧光、及间隔物部分(PPDK内含子)高表达的个体。将挑选出的个体(T0个体)移植至3号盆，在23～25℃的封闭型温室中于一定条件下进行栽培。使T0个体进行自身繁殖，对T1种子进行了采种。
(c)T1重组体的挑选
将T1种子无菌地接种于Linsmaier and Skoog培养基，对苗的基于GFP的荧光进行观察。根据导入基因的基因型(纯合型(纯合)/半合型(杂合)及无效(null)分离个体(无效))的分离比，挑选了推测导入基因的基因座数为1～2的品系。
通过使用了从T1品系的叶或根分离得到的total RNA的qPCR，确定了对象基因的表达水平。以纯合的表达水平相对于无效的表达水平之比的形式评价了表达水平。在纯合或无效的品系中，挑选了该比小(即，对象基因的表达抑制的程度大)的品系。上述qPCR的详细情况如下所述。
使用专用的软件(PrimerExpress、ABI)进行qPCR的引物及探针的设计，或者委托Sigma-Aldrich Japan进行。如1-1.(b)中的记载所述，根据从叶或根分离得到的total RNA合成了cDNA。使用稀释至2～5倍的cDNA、如上所述得到的引物、以及Taq Man FastAdvanced Master Mix(ABI)进行了qPCR。作为定量化的对照，对eukaryotic elongationfactor-1α基因(accession No.AF120093、efla)进行了扩增。作为定量化用探针，使用了报告色素-淬灭剂的组合(FAM-TAMURA(分析对象的基因)及VIC-TAMURA(对照))。示出了qPCR用的引物及探针的序列。在以以下的各基因作为对象的序列中，第1个是正向引物，第2个是反向引物，第3个是探针。
(NtLS)
NtLS_qFW1:CCGGTACTGGAAATGACCTTGA
NtLS_qRV1:ATCTAAGGCCTAAAGAGTGAGCAAAT
NtLS_P1:CCCTTCGTAGAACCGGAGATCGTTTAGCT
(NtBl1)
NtBl1_qFW1:GAGAAAACAAATGTAAGTACACCATTAGG
NtBl1_qRV1:GAAAAAGTTTGAATCTTCTTGCCAA
NtBl1_P1:GATTTGAAAGGGCGTTTGGGTATGGG
(NtREV)
NtREV1_qFW1:TCTCCAGGCTCCCCTGAAG
NtREV1_qRV1:TGTCCCCATGTGATAACTGTAGCT
NtREV1_P1:AACGTTGTCGCACTGGATCTGCCA
(#07437)
Nt_07437_1-F:ATGGCTACCCTACAAGCTTGAAA
Nt_07437_1-R:TTGCCAATGTGTAGTTGTTGTGG
Nt_07437_1-P:TCTTAACACAGCAACATCAGCAGAAGCAGC
(#15360)
Nt_15360_48821-F:ACTCCTGTTGAGAATGCACAAATAA
Nt_15360_48821-R:CCAGAAATATTAGTTTCTTCTCCTTGG
Nt_15360_48821-P:CCATCTGAAAATGCATAACCTGGAAGCTGC。
作为以上的挑选的结果，为了供于腋芽评价试验，对于表达受到了抑制的每个对象基因进行挑选得到的个体如下所述。
·NtREV：在评价了表达水平的10个个体的T1品系中，基因座数为1、且表现出明显的表达抑制的3个个体的T1品系(品系号：3、8及14)
·NtBl1：在评价了表达水平的15个个体的T1品系中，基因座数为1、且表现出明显的表达抑制的3个个体的T1品系(品系号：6、9及12)
·NtLS：在评价了表达水平的24个个体的T1品系中，基因座数为1、且表现出明显的表达抑制的3个个体的T1品系(品系号：10、15及19)
·#15360：在评价了表达水平的22个个体的T1品系中，基因座数为1或2、且表现出明显的表达抑制的3个个体的T1品系(品系号：11、14及17)
·#07437：在评价了表达水平的20个个体的T1品系中，基因座数为1、且表现出明显的表达抑制的3个个体的T1品系(品系号：1、10及22)。
将各重组体的T1品系组中的对象基因的表达水平之比(将无效的表达设定为1)示于以下。
·NtREV-品系3：0.56、品系8：0.57、品系14：0.74
·NtBl1-品系6：0.33、品系9：0.35、品系12：0.25
·NtLS-品系10：0.50、品系15：0.58、品系19：0.43
·#15360-品系11：0.07、品系14：0.10、品系17：0.08
·#07437-品系1：0.24、品系10：0.17、品系22：0.13。
(2-2.温室中的腋芽评价)
接种如上所述得到的各重组体的T1品系的种子，在封闭型温室或Koitotron(人工气候箱，小丝制作所)中进行栽培。将封闭型温室设定为保持23～25℃的室温的自然日长条件，将Koitotron设定为12小时日长、25℃(光照期)及18℃(黑暗期)的条件。在装有500mL/盆的肥料土的5号盆中栽培个体。肥料土的组成为：堆肥：40L，原野土：30L，赤玉土(小)：10L，赤玉土(中)：10L，蛭石：10L，肥料(S625)：1000g。
在从出蕾至开花前的期间中生长了12～13片真叶的时刻进行打顶，选择位于地上部的从下起第4片以上的真叶产生的腋芽作为评价对象。从打顶日起包括第1周在内，记录具有约5mm以上长度的茎的腋芽的数量，从芽根部通过手工操作摘取记录的对象的腋芽，测定了摘取的腋芽的新鲜重量(FW)。至无法确认新腋芽的发育为止，大约进行了5次，对腋芽的数量及重量进行了调查。
首先，将对抑制了NtREV表达的重组体(在封闭型温室中栽培)的腋芽发育进行评价的结果示于图1。对于抑制了NtREV表达的重组体的3个品系的全部纯合(在图1中，在品系数之后附加“H”)而言，与对应的无效(在图1中，在品系数之后附加“N”)相比，在二级腋芽的数量方面统计上显著减少，其中的2个品系未产生二级腋芽。另一方面，对于品系12的纯合而言，与对应的无效相比，除了一级腋芽的数量在统计上显示出显著增加这一点以外，在一级腋芽的数量及新鲜重量方面，未在纯合及无效之间确认到统计上的显著差异。需要说明的是，在图2～5中，“N”及“H”的含义与图1相同。
将对抑制了NtBl1表达的重组体(在Koitotron中栽培)的腋芽发育进行评价的结果示于图2。抑制了NtBl1表达的重组体的3个品系的全部纯合未产生二级腋芽，而对应的无效产生了二级腋芽。另一方面，在一级腋芽的数量及新鲜重量方面，未在纯合及无效之间确认到统计上的显著差异。
将对抑制了NtLS表达的重组体(在Koitotron中栽培)的腋芽发育进行评价的结果示于图3。对于抑制了NtLS表达的重组体的3个品系的全部纯合而言，与对应的无效相比，在二级腋芽的数量方面统计上显著减少。另外，与对应的无效相比，1个纯合在二级腋芽的新鲜重量方面统计上显著减少，其余2个纯合虽然在统计上不显著，但显示出新鲜重量减少。另一方面，在一级腋芽的数量及新鲜重量方面，未在纯合及无效之间确认到统计上的显著差异。
将对抑制了#15360表达的重组体(在Koitotron中栽培)的腋芽发育进行评价的结果示于图4。对于抑制了#15360表达的重组体的3个品系的全部纯合而言，与对应的无效相比，在二级腋芽的数量方面统计上显著减少。另外，与对应的无效相比，2个纯合在二级腋芽的新鲜重量方面统计上显著减少，其余1个纯合虽然在统计上并不显著，但显示出新鲜重量的减少。另一方面，与对应的无效相比，品系17的纯合除了在一级腋芽的新鲜重量方面统计上显著增加这一点以外，在一级腋芽的数量及新鲜重量方面未在纯合及无效之间确认到统计上的显著差异。
将对抑制了#07437表达的重组体(在Koitotron中栽培)的腋芽发育进行评价的结果示于图5。在抑制了#07437表达的重组体的3个品系中，与对应的无效相比，品系1的纯合在二级腋芽的数量及新鲜重量方面统计上显著减少。另外，与对应的无效相比，其余2个品系的纯合虽然在统计上并不显著，但显示出二级腋芽的数量及新鲜重量的减少。另一方面，在一级腋芽的数量及新鲜重量方面，未在纯合及无效之间确认到统计上的显著差异。
根据以上结果可知，5个对象基因的表达抑制未抑制一级腋芽的发育，而可以选择性地抑制二级腋芽的发育。
〔3.导入对象基因的突变对于腋芽发育的影响的确认(1)〕
(3-1.通过EMS处理生成的突变体)
(a)突变体的筛选
通过对种子进行甲磺酸乙酯(EMS)处理，制备了烟草(品种：Tsukuba 1号)的突变体实验对象集合(TUM)(文献：平成23年日本植物病理学会大会p234、烟草突变体实验对象集合的制备)。该突变体实验对象集合包含突变体自身繁殖后代种子(M2主体种子)组和主体DNA(bulk DNA)组，所述突变体自身繁殖后代种子(M2主体种子)组是对数千个种子进行以EMS作为突变原的处理，由每个培育出的个体(M1代)得到的，所述主体DNA组是接种上述M2种子，从培育出的各品系8个个体的幼苗中提取而得到的。以该DNA样品作为模板，对突变体文库的基因组进行了Single-strand conformation polymorphism(SSCP)分析或PCR扩增片段的直接测序，基于该结果，挑选了NtREV或NtLS中具有突变的突变体。在SSCP中，通过使用了结合荧光色素的PCR引物的PCR对靶部位进行扩增，使用毛细管电泳装置(ABI3130xlDNA analyzer)检测了扩增得到的片段。使用QIAGEN Multiplex PCR Kit(QIAGEN)按照试剂盒附带的手册实施了PCR。该PCR引物的序列如下所述。
(NtREV、S基因组)
Nt_in0_F1:TTGGTTTGGGATTTTGAGGTTTGAGG、及Nt_ex1_R1:TCCATCACTGATCTAACTAATCCAAG的组合
Ns_in1_F1:TTTGGAATTGAGGGTGAACATTGTGC、及Ns_in2_R1:ACGTTACCATTCGTCTACAGTAAGC的组合
Ns_in2_F1:CCAATAAACAAGAAACAGATGATGG、及Ns_in3_R1:GAATGGACACCATAGACGGAAAGGA的组合
Ns_in3_F1:TTTCCGTCTATGGTGTCCATTCTCC、及Ns_in4_R1:GAGACATGGCAATACTGAATTTTCA的组合
Ns_in4_F1:GAAAATTCAGTATTGCCATGTC、及Ns_in6_R1:AGCCTACGTGAAGATTGATGAGAAG的组合
(NtREV、T基因组)
Nt_in0_F1:TTGGTTTGGGATTTTGAGGTTTGAGG、及Nt_ex1_R1:TCCATCACTGATCTAACTAATCCAAG的组合
Nt_in1_F1:TCGATTGGGTTGTATGAGTTAACCGT、及Nt_in2_R1:GTTACCATAAGCTGTGGAATATCAGG的组合
Nt_in2_F1:AACCAATGGACAAGAAACGGATGGCA、及Nt_in4_R1:TTTAGCTATCCAGTCAAAGAGGCACG的组合
Nt_in4_F1:AAAAAAATTCAGTATTGCCACGTGC、及Nt_in6_R1:AGCCTACGTGAAGATTGATGAGAAA的组合
(NtLS、S基因组)
LS_F2_seq:ATTTCCCCTCCTCCATCATTG、及LS1-R1:CAACAACATTAGATGGTGTCAAG的组合
LS1-F2:CTTGACACCATCTAATGTTGTTG、及NtLS_QPCR_RV1:ATCTAAGGCCTAAAGAGTGAGCAAAT的组合
LS1,2-F3:TTCGTAGAACCGGAGATCGT、及LS1,2_R3:GCAAAGTTGCTTCCAATGAAT的组合
LS1,2_F4:GTGGAGGCTTTGGATTATTATG、及N.t_LS_TRV_R2:GAAGACCTCTTTGTCCTTCACCATGCAG的组合
(NtLS、T基因组)
LS_F2_seq:ATTTCCCCTCCTCCATCATTG、及LS2-R1:AACATTAGATGATGCATTAGGTGT的组合
LS2-F2:ACACCTAATGCATCATCTAATGTT、及NtLS_QPCR_RV1:ATCTAAGGCCTAAAGAGTGAGCAAAT的组合
LS1,2-F3:TTCGTAGAACCGGAGATCGT、及LS1,2_R3:GCAAAGTTGCTTCCAATGAAT的组合
LS1,2_F4:GTGGAGGCTTTGGATTATTATG、及N.t_LS_TRV_R2:GAAGACCTCTTTGTCCTTCACCATGCAG的组合。
通过对来自于M2代的突变个体的基因组的PCR扩增片段进行克隆、并且确认该片段的碱基序列，进行了导入了突变的基因的序列鉴定。通过导入了突变的基因表达的多肽与野生型蛋白质(WT)的差异如下所述。
通过S基因组中导入了突变的NtREV表达的多肽(MT、Ns1630突变体、序列号36)与野生型蛋白质相比具有以下差异。
·MT：111aa、WT：838aa
由于第112号的谷氨酰胺(Q)变为终止密码子，因此全长缩短为111aa。
通过T基因组中导入了突变的NtREV表达的多肽(Nt1605突变体、序列号37)与野生型蛋白质相比具有以下差异。
·MT：116aa、WT：839aa
由于117号的谷氨酰胺(Q)变为终止密码子，因此全长缩短为116aa。
通过T基因组中导入了突变的NtREV表达的多肽(Nt5850突变体、序列号38)与野生型蛋白质相比具有以下差异。
·MT：68aa、WT：839aa
由于69号的谷氨酰胺(Q)变为终止密码子，因此全长缩短为68aa。
通过T基因组中导入了突变的NtLS表达的多肽(Nt1145突变体、序列号39)与野生型蛋白质相比具有以下差异。
·MT：398aa、WT：410aa
由于399号的谷氨酰胺(Q)变为终止密码子，因此全长缩短为398aa。
通过T基因组中导入了突变的NtLS表达的多肽(Nt1025突变体、序列号40)与野生型蛋白质相比具有以下差异。
·MT：145aa、WT：410aa
由于146号的谷氨酰胺(Q)变为终止密码子，因此全长缩短为145aa。
通过S基因组中导入了突变的NtLS表达的多肽(Ns369突变体、序列号41)与野生型蛋白质相比具有以下差异。
·MT：163aa、WT：407aa
由于164号的谷氨酰胺(Q)变为终止密码子，因此全长缩短为163aa。
(b)从M2突变体组挑选希望的突变体
从推测靶基因中具有突变的M2突变体组中按照以下步骤制备了T基因组及S基因组两者中纯合地具有靶基因突变的突变体(T+S+)、以及T基因组及S基因组两者中不具有靶基因的突变的突变体(T-S-)。
首先，从M2突变体组中挑选了以下的4个群组。
T基因组中纯合地具有靶基因突变的M2突变体(T+)
S基因组中纯合地具有靶基因突变的M2突变体(S+)
T基因组中不具有靶基因突变的M2突变体(T-)
S基因组中不具有靶基因突变M2突变体(S-)
然后，使T+及S+交配而制备得到的F1品系进行自身繁殖，制备了目标的F2突变体(T+S+)。同样地制备了T-S-。
在上述步骤中，为了确定有无基因组上的突变，按照下一段的记载实施了CycleavePCR法。作为确认NtREV基因的突变的CycleavePCR中的模板，使用了通过简易提取法提取出的基因组DNA。作为确认NtLS基因的突变的CycleavePCR中的模板，将从基因组DNA(T及S基因组分别)通过PCR扩增而得到的片段稀释至300～500倍来使用。该PCR使用TksGflex(商标)DNA polymerase(Takara Bio)实施，该PCR的反应条件及引物如下所述。
(反应条件)
94℃下30秒钟
以98℃下10秒钟、55℃下15秒钟及68℃下90秒钟作为1个循环，35个循环
68℃下90秒钟
(引物)
T基因组
NtLS_prePCR_Ntom_F1:CCCAGACCCCCTTTTCCTCT
NtLS_prePCR_Ntom_R1:AATTTCCCTTATAATTTAACGCC
S基因组
NtLS_prePCR_Nsyl_F1:CCCTAGAGAGACCCCTTTTTC
NtLS_prePCR_Nsyl_R1:GGGTTTTAAATTTAACGCCAA。
使用从Takara Bio的网页可用的Cycleave(注册商标)RPCR Assay Designer(SNPs)设计了CycleavePCR法用的引物及探针(表1)。与该引物及探针一起使用CycleavePCR Reacrion Mix(Takara Bio)，按照该公司提供的手册实施了CycleavePCR法。使用Applied Biosystems(注册商标)StepOnePlus(商标)实时PCR系统(Thermo Fisher)实施了PCR反应。
[表2]
(c)农田中的腋芽评价
农田中的栽培
在烟叶研究所田地中，在通常的耕作期(3月播种、4月种植)，以垄长16m、垄间120cm、株间43cm、37株/垄的条件下，通过高垄及多栽培法栽培了突变体的各品系。1个品系的栽培分配1垄，根据外观判断并预挑选在从移植起1个月后显示出相同的生长程度的10～15个个体，将其中的10个个体继续供于试验。试验中没有使用任何腋芽抑制剂(例如，CONTACT)。
开花期的确定
在开花的时期确定了地上叶数、以及在即将打顶前确定了推定的开花期。对第1花枝+1～4片的叶进行切除来打顶，在进行比较、评价的品系之间使地上叶数一致。
腋芽发育的评价
从打顶及打顶日起包括第1周在内，总计7次记录茎长度达到约5mm的腋芽的数量，通过手工操作从芽根部摘取记录的对象的腋芽，测定了摘取的腋芽的新鲜重量(FW)。对于一级腋芽、二级腋芽及三级腋芽，对每个个体总结了记录及测定的结果。
将对在NtREV中导入了突变的突变体的腋芽发育进行评价的结果示于图6。NtREV_Homo品系(T+S+)未产生二级腋芽及三级腋芽。NtREV_Null品系(T-S-)产生了二级腋芽及三级腋芽(与T+S+相比在统计上有显著差异)。另一方面，在一级腋芽的数量及新鲜重量方面，未在2组之间确认到在统计上有显著差异。
将对在NtLS中导入了突变的突变体的腋芽发育进行评价的结果示于图7。与NtLS_Null品系(T-S-)相比，NtLS_Homo品系(T+S+)在二级腋芽的数量及新鲜重量方面统计上显著减少。而且，未产生三级腋芽(与NtLS_Null品系在统计上具有显著差异)。另一方面，在一级腋芽的数量及新鲜重量方面，未在2组之间确认到在统计上有显著差异。
根据以上的结果可知，对于NtREV或NtLS的突变体而言，与基因表达的抑制相同，可以选择性地抑制二级腋芽(及三级腋芽)的发育。
(3-3.通过CRISPR/Cas9system生成的NtBl1的突变体)
(a)转化的准备
作为转化土壤杆菌的载体，使用了双元载体pRI-201-AN(Takara Bio)。在pRI-201-AN的NdeI-SalI之间导入了对植物用途进行了密码子最优化的pcoCas9(参考文献2)，在KpnI-BamHI之间导入了sgRNA表达盒。作为引导序列GN20GG用的启动子，使用了AtU6-1(参考文献3)，作为引导序列AN20GG用的启动子，使用了AtU3B(参考文献4)，而且作为scaffold-polyT序列，使用了参考文献2中报道的序列。将构建而成的载体的示意图示于图8(图中的target sequence为本文中的引导序列)。即，除了3’末端的PAM序列(NGG)以外的引导序列设计为在启动子及scaffold-polyT序列之间插入的sgRNA表达盒。委托从Life Technology的GeneArt(注册商标)Strings(商标)DNA Fragments合成了在5’末端添加了KpnI位点且在3’末端于末端侧添加了BamHI位点的sgRNA表达盒(化学式1)。委托Takara Bio进行了在5’末端添加了NdeI位点且在3’末端添加了SalI的Cas9的人工合成，获得了该Cas9(化学式2及3)。
[化学式1]
下划线部分表示引导序列，其上游表示AtU3B启动子序列，其下游表示scaffold-polyT序列，末端小写字母表示KpnI、BamHI的限制酶序列。
[化学式2]
Cas9序列
序列接下页
[化学式3]
Cas9序列接上页
在跨过2页以上的Cas9序列中，下划线部分表示NdeI、SalI序列。
使用导入了Cas9及sgRNA表达盒的pRI201-AN，通过电穿孔对土壤杆菌LBA4404进行了转化。在含有25μg/ml的卡那霉素的AB板上使土壤杆菌增殖，分离了单菌落的土壤杆菌。
(b)烟草的转化、以及转化体的栽培
将从播种第10天的烟草(品种：SR-1)上采集的子叶片与如上所述得到的转化后的土壤杆菌共存培养3天。然后，使用含有抗菌剂(头孢噻肟)的蒸馏水清洗子叶片，从而从子叶片上除去土壤杆菌。将清洗后的子叶片在含有抗菌剂的Linsmaier and Skoog培养基中培养4天，由此完全除去了土壤杆菌。然后，将子叶片转移至含有抗生素(卡那霉素)的Linsmaier and Skoog培养基中进行培养，得到了卡那霉素耐性的再分化个体(幼苗)。将幼苗转移至Linsmaier and Skoog培养基使其生根。从生根的幼苗中选择高表达(与对照的eukaryotic elfa相比为2倍以上的表达量)Cas9mRNA的个体，移植至装有移植用土(堆肥：40L、原野土：30L、赤玉土(小)：10L、赤玉土(中)：10L、蛭石：10L、肥料(S625)：1000g)的3号盆中，使其生长。
(c)有无突变及突变序列的确认
以从烟草转化体的叶中提取出的基因组DNA作为模板，使用Tks Gflex(商标)DNApolymerase(Takara Bio)进行了PCR。PCR中的反应条件及引物如下所述。
(反应条件)
94℃下30秒钟
以98℃下10秒钟、55℃下15秒钟及68℃下60秒钟作为1个循环，40个循环
68℃下60秒钟
(引物)
T基因组
NtBl1-1_2A_F1:AAGTATTACTACTACAAAATTCCAACG、及
Nb_Bl1_2A_R1:CCATCTGATGAAGAACAACTTGC的组合
S基因组
NtBl1-2_1A_F1:TTAAACACTAGAGAGTGAGAGAGTGC、及
NtBl1-2_2A_F1:CAGATGTTTAATTATTAAGACAAAGTTCC的组合。
在PCR反应后，在下述条件下进行了变性/退火。变性：95℃5分钟，退火：85℃1秒钟/85℃1秒钟、60℃1秒钟、30℃恒定。85～60℃的Ramp Rate(变温速率)为5％(0.1℃/秒的下降率)，60～30℃的Ramp Rate为10％(0.1℃/秒的下降率)。在10μl的反应体系中使用1U的T7endonuclease I(New England Biolabs)处理变性/退火后的5μl的PCR产物，通过电泳进行分离，确认了PCR产物是否受到酶的切断。另行使用Zero Blunt TOPO PCR CloningKit克隆PCR产物，确定了其碱基序列。
(d)重组体的挑选
使T基因组及S基因组两者中具有突变(缺失或插入1碱基以上)的T0世代个体自身繁殖，进行采种，由此得到了T1品系。与(c)同样地确认了T1品系的个体中有无突变。根据确认的结果，使在T基因组及S基因组中纯合地具有突变的T1品系(T+S+)的个体自身繁殖而得到T2品系(T+S+)的个体，将其用于试验。
得到的T2品系的个体中的突变体多肽
·2A-1_121、2A-1_126、2A-133_1、2A-161_17(Bl1-1-T基因组：1b deletion)
WT由336个氨基酸构成，与此相对，生成在至107个氨基酸为止相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号92、94、96、106)
·2A-1_121、2A-1_126(Bl1-2-S基因组：5b deletion)
WT由337个氨基酸构成，与此相对，生成在至106个氨基酸为止相同的氨基酸的基础上添加了无关的3个氨基酸(LEY)的多肽(序列号91、93)
·2A-133_1(Bl1-2-S基因组：3b deletion)
生成WT的337个氨基酸中第107号N(天冬酰胺)缺失的337个氨基酸的多肽(序列号95)
·2A-161_8、2A-161_122(Bl1-1-T基因组：22b deletion)
WT由336个氨基酸构成，与此相对，生成在至101个氨基酸为止相同的氨基酸的基础上添加了无关的11个氨基酸(EILNSRKSLWD)的多肽(序列号104、108)
·2A-161_8、2A-161_17、2A-161_122(Bl1-2-S基因组：2b deletion)
WT由337个氨基酸构成，与此相对，生成在至106个氨基酸为止相同的氨基酸的基础上添加了无关的4个氨基酸(KLEY)的多肽(序列号103、105、107)。
(e)温室中的腋芽评价
在温室中栽培(d)中得到的T2品系的个体(T+S+)，对腋芽进行了评价。评价的详细情况如2-2.中记载所述。
将对在NtBl1中导入了突变的突变体的腋芽发育进行评价的结果示于图9及10。如图9及10所示，在NtBL1基因中导入了突变的个体均在一级腋芽的数量及重量方面未显示出与野生型的显著差异，而在二级腋芽的数量及重量方面与野生型相比显示出在统计上显著减少。
根据以上的结果可知，对于NtBl1的突变体而言，与基因表达的抑制相同，可以选择性地抑制二级腋芽的发育。
〔4.对象基因中导入的突变对腋芽的发育位置的影响的确认〕
与WT(SR-1)同样地在Koitotron中栽培通过CRISPR/Cas9system制备的NtBl1突变体。在从打顶起1周后确认了一级腋芽的发育位置(图11上)。从Tsukuba 1号突变体实验对象集合中进行挑选，将制备的NtLS突变体(Homo品系)与NtLS突变体(Null品系)同样地在农田中进行栽培。在移植第63天确认了一级腋芽的发育位置(图11下)。
如图11所示，在2个突变体中，一级腋芽形成于偏离叶腋的位置。因此，从这些突变体上摘取一级腋芽是极其容易的。其原因是由于在摘取腋芽时会产生如下问题。在摘取腋芽时，如果腋芽形成于叶腋，则又是会对带有收获叶的侧枝造成损伤。这样的损伤可能成为病原体的侵入途径，因此对叶的产量及品质造成严重的不良影响。
〔5.对象基因中导入的突变对于烟草植物的生长的影响的确认〕
(a)试验对象
对于使3-2.(c)中评价的品系、以及3-3.(e)中评价的Bl1_2A-1_121自身繁殖而得到的后代的T3品系，如下所述研究了各基因的突变对于植物体的生长的影响。作为比较组，使用了用于制备各突变体的品种。
(b)条件
在温室(25℃恒定)中，将种植于3号盆中的各个体栽培至出蕾。肥料土的组成与2-2.相同。在出蕾时，测定了各个体的株高、新鲜重量及干燥重量、以及各叶位的叶长及叶宽。株高是从盆的肥料土表面至最上位花枝的根部的长度。新鲜重量是刚刚收获了16片(NtLS突变体、NtREV突变体)或14片(NtBl1突变体)的地上叶后、收获叶及植物体的总重量。干燥重量是测定了新鲜重量后的收获叶及植物体在70℃及相对湿度7％下进行热风干燥后的重量。叶长是从叶柄至叶的前端在纵方向上的长度，叶宽是叶的横宽的最大长度。为了确定叶长及叶宽，按照从最下位叶(1)起至上位的顺序赋予表示叶位的编号。
(c)结果
如图12(突变体：n＝3、WT(Tsukuba 1号)：n＝2)、图13(突变体及WT(Tsukuba 1号)：n＝3)、以及图14(突变体：n＝2、WT(Tsukuba 1号)：n＝3)分别表明，对于株高、新鲜重量及干燥重量，在突变体组及对照组之间没有确认到统计上有显著差异。而且，根据这些图可知，对于叶长及叶宽，在突变体组及对照组之间也没有确认到显著差异。
以上结果表明，各突变体显示出与对照组实质上相同程度的生长及叶的产量。因此可知，对于这些实施例的突变体而言，由于(1)腋芽处理容易、(2)不发生产量降低、(3)植物体的生存性高等原因，作为以收获叶为目的的烟草植物，是极其有用的。
〔6.对象基因中导入的突变对于腋芽发育的影响的确认(2)〕
制备具有以下的特征(profile)的突变体，对该突变体的成长状态、腋芽发育及使用了腋芽抑制剂的情况下的腋芽发育进行了评价。
LS_21_Null：是使Ns369突变体与Nt1025突变体交配而得到的F1进行了2次自身繁殖而成的F3个体，是S基因组中纯合地具有Ns369突变体(表达序列号41的多肽)的突变、且T基因组中纯合地具有Nt1025突变体(表达序列号40的多肽)的突变的突变体(T+S+)。
LS19_WT：是使Ns369突变体与Nt1025突变体交配而得到的F1进行了2次自身繁殖而成的F3个体，是T基因组、S基因组两者中不具有LS基因的突变的突变体(T-S-)
LS15_Null、LS85_Null：是使Ns369突变体与Ns1145突变体交配而得到的F1进行2次自身繁殖而成的F3个体，是S基因组中纯合地具有Ns369突变体(表达序列号41的多肽)的突变、且T基因组中纯合地具有Nt1145突变体(表达序列号39的多肽)的突变的突变体(T+S+)。
LS_57_WT：是使Ns369突变体与Ns1145突变体交配而得到的F1进行2次自身繁殖而成的F3个体，是T基因组、S基因组两者中不具有LS基因的突变的突变体(T-S-)
REV_26_Nu-W及REV_89_Nu-W：T基因组中纯合地具有Nt1605突变体(表达序列号37的多肽)的突变、且S基因组中纯合地具有Ns1630突变体(表达序列号39的多肽)的突变的突变体(T+S+)与K326交配而得到了F1。使K326与F1进行1次回交而得到的BC1F1进行2次自身繁殖，得到了BC1F3个体。在该BC1F3个体当中，将T基因组中纯合地具有Nt1605突变体(表达序列号37的多肽)的突变、且S基因组中不具有REV基因的突变的突变体(T+S-)作为REV_26_Nu-W及REV_89_Nu-W。
REV_26_Nu-He及REV_89_Nu-He：T基因组中纯合地具有Nt1605突变体(表达序列号37的多肽)的突变、且S基因组中纯合地具有Ns1630突变体(表达序列号39的多肽)的突变的突变体(T+S+)与K326交配而得到了F1。使K326与该F1进行1次回交而得到的BC1F1进行2次自身繁殖，得到了BC1F3个体。在该BC1F3个体当中，将T基因组中纯合地具有Nt1605突变体(表达序列号37的多肽)的突变、且S基因组中杂合地具有Ns1630突变体(表达序列号39的多肽)的突变的突变体(T+S+/-)作为REV_26_Nu-He及REV_89_Nu-He。
REV35_WT：T基因组中纯合地具有Nt1605突变体(表达序列号37的多肽)的突变、且S基因组中纯合地具有Ns1630突变体(表达序列号39的多肽)的突变的突变体(T+S+)与K326交配而得到了F1。使K326与该F1进行1次回交而得到的BC1F1进行2次自身繁殖，得到了BC1F3个体。在该BC1F3个体当中，将T基因组、S基因组两者中不具有REV基因的突变的突变体(T-S-)为REV35_WT。
REV_F3_Null：是使Nt1605突变体与Ns1630突变体交配而得到的F1进行了2次自身繁殖而成的F3个体，是T基因组中纯合地具有Nt1605突变体(表达序列号37的多肽)的突变、且S基因组中纯合地具有Ns1630突变体(表达序列号39的多肽)的突变的突变体(T+S+)。
REV_F3_WT：是使Nt1605突变体与Ns1630突变体交配而得到的F1进行了2次自身繁殖而成的F3个体，是T基因组、S基因组两者中不具有REV基因的突变的突变体(T-S-)。
(1)生长状态的评价
调查中使用了3个品系的LS突变体无效品系、2个品系的作为该突变体无效品系的对照的品系、以及作为突变体的亲本品种的Tsukuba 1号。作为突变体无效品系，使用了LS_21-1_Null、LS_15_Null、以及LS_85_Null。作为突变体无效品系的对照，作为突变体无效品系LS_21-1_Null的对照，使用了F2分离品系中LS不具有突变的LS_19_WT，作为突变体无效品系LS_15_Null及LS_85_Null的对照，使用了F2分离品系中LS不具有突变的LS_57_WT。
在烟叶研究所田地中，于稍晚于通常的耕作期(3～4月播种、5月种植)在株间43cm、垄间120cm的条件下，通过高垄及多栽培法栽培了上述的各品系的个体。作为肥料，使用了2000kg/10a的堆肥，120kg/10a的Agri 622。对栽培的各品系的个体的开花天数、株高、地上叶数、鲜叶重、以及干燥重量进行了评价。需要说明的是，在本说明书中，开花天数是从播种日至第一朵花开花的天数。株高是指从地面至最上位花枝的根部的高度。地上叶数是指位于从地上至最接近第一花枝下方的叶的位置的叶的总数。将地上叶的全部叶在干燥前的总重量作为鲜叶重，将地上叶的全部叶在干燥后的重量作为干燥重量。每个品系各评价了6个个体，计算出各自的评价值的平均值和标准偏差。对突变体无效品系与对照之间在各评价值方面是否存在统计上的显著差异进行了调查。
参照图15对结果进行说明。除了LS_15_Null的株高比对照(LS_57_WT)显著增高、LS_15_Null的开花天数比对照(LS_57_WT)显著缩短这一点和LS_85_Null的地上叶数比对照(LS_57_WT)显著增多这一点以外，对照与突变体无效品系之间在评价值方面没有观察到显著差异。由此可以认为，LS基因的突变没有对生长造成很大影响。
(2)腋芽发育的评价
与(1)同样地栽培了各个体。作为LS突变体及其对照，使用突变体无效品系(LS_21)及其对照(LS_19_WT)、LS无效突变体(LS_15、LS_85)及其对照(LS57_WT)进行了试验。LS_21通过在2块田中栽培而进行了反复试验。作为REV突变体及其对照，使用了进行了回交的BC2F3分离品系(回交亲本：K326)的4个品系(REV_26_Nu-W、REV_26_Nu-He、REV89_Nu-W、REV_89_Nu-He)及其对照(REV_35_WT)。另外，作为REV突变体及其对照，使用了未进行回交的REV无效突变体(REV_F3_Null)及其对照(REV_F3_WT)。另外，试验中使用了作为各突变体的亲本品种的Tsukuba 1号及BC2F3分离品系的回交亲本K326。
在开花期于最接近第一花枝下方进行打顶，调查了地上叶上产生的腋芽。打顶当日进行第1次调查，随后每1周进行总计8次调查。以腋芽的茎长为5mm以上的腋芽作为调查对象，对每个个体记录采集腋芽时该腋芽的位置，在采集腋芽后测定了该腋芽的重量。一级腋芽、二级腋芽及三级腋芽分别单独进行总结、调查及统计。
以下，参照图16及17对结果进行说明。图16示出了LS突变体的评价结果，图17示出了REV突变体的评价结果。
如图16所示，3种LS突变体无效品系与对照相比，均观察到一级腋芽的数量多、鲜叶重(FW)少的倾向。作为LS突变体无效品系的一级腋芽的数量多的原因，作为可能性可以举出，与对照相比，LS突变体无效品系中具有地上叶数量多的倾向(图15)。另一方面，二级腋芽、三级腋芽比对照大幅减少、或者完全未形成。根据以上的结果可以确认，在LS具有不同的突变的突变体中，二级腋芽及二级以上腋芽的腋芽形成受到抑制。
如图17所示，在REV基因的全部等位基因中导入了突变的REV_F3_Null中，完全未形成二级腋芽及三级腋芽。另外，在T基因组中的2个等位基因及S基因组中的1个等位基因中具有突变的2个品系(REV_26_Nu-He、REV_89_Nu-He)中，虽然形成了二级腋芽，但与对照(REV_35_WT)相比，数量及鲜叶重均减少为1/2以下，该减少在统计上是显著的。
另一方面，在T基因组中的2个等位基因中具有突变、且S基因组中不具有突变的2个品系(REV_26_Nu-W、REV_89_Nu-W)中，二级腋芽的数量及鲜叶重与对照(REV_35_WT)相同。根据以上结果可以确认，关于REV基因，即使不在T基因组及S基因组中的全部等位基因中导入突变，只要在总计3个等位基因中导入了突变，就具有二级腋芽抑制效果。
(3)使用了腋芽抑制剂的情况下的腋芽发育
使用装有5L肥料土的菊盆(内径25cm、高24cm)，在大棚中进行了栽培。肥料土的组成为堆肥：40L，原野土：10L，桐生砂：15L，赤玉土(小)：15L，赤玉土(中)：15L，蛭石：10L，BareS625(肥料)：1000g。在5月进行播种，移植后经过了1.5个月左右后追加了2L肥料土。在开花期切除第一花枝下方2片为止(打顶)，3天后对每株喷洒了20ml的稀释至30倍的CONTACT(OAT AGRIO)。喷洒4～7天后调查了CONTACT是否接触一级腋芽，记录未接触CONTACT的一级腋芽，在伸长后出去未接触CONTACT的一级腋芽。此后，将从CONTACT未接触一级腋芽的部位形成的二级腋芽排除在调查对象以外。CONTACT未接触一级腋芽的部位在每株平均存在1～2个部位。喷洒CONTACT后，每周1次、共13次调查了二级腋芽的产生。每个品系的平均着叶数为25～31片不等，因此对单位着叶数产生的二级腋芽的比例进行了比较。对于作为LS的无效突变体的LS_15_null及LS_85_null、作为它们的对照的LS中不具有突变的LS_77_wt、作为LS的无效突变体的LS_14_null及LS24_null、作为它们的对照的LS中不具有突变的LS_19_wt进行了比较。
参照图18对结果进行了说明。喷洒CONTACT后产生的二级腋芽的数量(单位着叶数)因LS基因的突变而在统计上显著地大幅减少。
〔7.对象基因中导入的突变对于腋芽发育的影响的确认(3)〕
(7-1.REV突变体及#15360突变体)
使用CRISPR/Cas9system制备新的突变体，评价了腋芽发育。制备的步骤按照3-3.的记载进行，因此仅对3-3.的变更点进行说明。
(a)转化的准备
在转化土壤杆菌的载体的构建中，委托Life Technology进行了sgRNA表达盒的合成。得到的sgRNA表达盒的碱基序列如下所述。
[化学式4]
REVG2
[化学式5]
REVG5
[化学式6]
ROXG1
在以上的3个sgRNA表达盒中，下划线部分表示引导序列，其上游表示AtU6-26启动子序列，其下游表示scaffold-polyT序列，末端小写字母表示KpnI、BamHI的限制酶序列。
(b)烟草的转化及转化体的栽培
烟草植物体的转化及转化体的栽培除了基于Cas9mRNA水平进行了个体的挑选这一点以外，如3-3.(b)的记载所述。
(c)有无突变及突变序列的确认
如3-3.(c)的记载所述实施了栽培的转化体中有无突变及突变序列的确认。设计了使位于基因组DNA上的包含引导序列的区域特异性扩增的引物。将各引物的序列示于以下的表中。
[表3]
PCR的反应条件如下所述。
(REVG2)
94℃下60秒钟
以98℃下10秒钟、60℃下15秒钟及68℃下50秒钟作为1个循环，45个循环
(REVG5、ROXG1)
94℃下60秒钟
以98℃下10秒钟、62℃下15秒钟及68℃下50秒钟作为1个循环，45个循环。
(d)重组体的挑选
使T基因组及S基因组两者中具有突变(缺失或插入1个碱基以上)的T0世代个体自身繁殖，进行采种，得到了T1品系。使用表3的引物确认了T1品系的个体中有无突变及其序列。挑选S基因组及T基因组两者的等位基因的4个部位全部具有突变的T1个体，用于试验。得到的T1个体的详细情况如下所述。
REV_G2-15
T基因组：缺失了6个碱基CAAGGC。WT由839个氨基酸构成，与此相对，生成由缺失了第175～176号这2个氨基酸(KA)的837个氨基酸构成的多肽(序列号65)。
S基因组：插入了1个碱基A。WT由838个氨基酸构成，与此相对，生成在至176个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的5个氨基酸(YRNCC)的多肽(序列号64)。
REV_G2_94
T基因组：插入了1个碱基T。WT由839个氨基酸构成，与此相对，生成在至176个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的5个氨基酸(YRNCC)的多肽(序列号67)。
S基因组：插入了1个碱基T。WT由838个氨基酸构成，与此相对，生成在至176个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的5个氨基酸(YRNCC)的多肽(序列号66)。
REV_G5_18
T基因组：插入了1个碱基C。WT由839个氨基酸构成，与此相对，生成在至212个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的4个氨基酸(MWSC)的多肽(序列号68)。
S基因组：插入了5个碱基TCGAC。WT由838个氨基酸构成，与此相对，生成在至212个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的7个氨基酸(RHVVLLV)的多肽(序列号69)。
REV_G5_59
T基因组：缺失了28个碱基。WT由839个氨基酸构成，与此相对，生成在至211氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的54个氨基酸(QRLLRSSKIDLLGSEIAGTLKFSQCFLQEMEQLNFCTRRYMLLPPWLLHVIFGL)的多肽(序列号71)。
S基因组：缺失了3个碱基GCA。WT由838个氨基酸构成，与此相对，生成由第212号的1个氨基酸(A)缺失的837个氨基酸构成的多肽(序列号70)。
ROX_G1-1(15360_G1-1)、ROXG1-30(15360_G1-30)
T基因组：插入了1个碱基A。WT由165个氨基酸构成，与此相对，生成在至40个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的3个氨基酸(KKA)的多肽(序列号123、125)。
S基因组：缺失了1个碱基A。WT由168个氨基酸构成，与此相对，生成在至40个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的14个氨基酸(EGIESVIVSRFCRV)的多肽(序列号122、124)。
ROX_G1-131(15360_G1-131)
T基因组：缺失1个碱基A。WT由165个氨基酸构成，与此相对，生成在至40个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的14个氨基酸(EGIESVIVSRFCRV)的多肽(序列号129)。
S基因组：插入了39个碱基及13个碱基。WT由168个氨基酸构成，与此相对，生成在至39个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的4个氨基酸(FLCG)的多肽(序列号128)。
ROX_G1-46(15360_G1-46)
T基因组：缺失了20个碱基。WT由165个氨基酸构成，与此相对，生成在至36个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的3个氨基酸(ENQ)的多肽(序列号127)。
S基因组：插入了1个碱基G。WT由168个氨基酸构成，与此相对，生成在至40个氨基酸为止与WT相同的氨基酸序列的基础上添加了无关的3个氨基酸(EKA)的多肽(序列号126)。
(e)温室中的腋芽发育的评价
在温室中栽培(d)中得到的T1品系的个体，如2-2.中记载所述进行了评价。将REV突变体的评价结果示于图19及20，将#15360突变体的评价结果示于图21。
如图19及20所示，在REV中导入了突变的全部4个品系中，在一级腋芽的数量及新鲜重量方面与野生型之间没有显著差异，二级腋芽的数量及新鲜重量与野生型相比在统计上显著减少。如图21所示，在#15360中导入了突变的全部4个品系中，在一级腋芽的数量及新鲜重量方面未显示出与野生型具有显著差异，二级腋芽的数量及新鲜重量与野生型相比显示出统计上显著减少。根据以上的结果可知，对于REV而言，不仅是从通过EMS处理制备的TUM中挑选的突变体，而且通过CRISPR/Cas9system制备的多个不同的突变体也可选择性地抑制二级腋芽的发育。对于#15360而言，在通过CRISPR/Cas9system制备的多个不同的突变体中，与基因表达的抑制相同，确认了二级腋芽的发育受到选择性抑制。
(7-2.Bl1突变体及LS突变体)
(a)转化的准备
在转化土壤杆菌的载体的构建中，委托Life Technology从GeneArt(注册商标)Strings(商标)DNA Fragments合成在5’末端添加了KpnI位点且在3’末端于末端侧添加了BamHI位点的sgRNA表达盒。得到的sgRNA表达盒的碱基序列如下所述。
[化学式7]
2G-35_10、2G-37_i03、3G-126_10、2G-126_i39
在上述序列中，下划线部分表示引导序列，其上游表示AtU6-1启动子序列，其下游表示scaffold-polyT序列，末端小写字母表示KpnI、BamHI的限制酶序列。
另外，利用Eurofinsgenomics的人工基因合成服务合成了在5’末端添加了KpnI位点、且在3’末端于末端侧添加了BamHI位点的sgRNA表达盒。得到的sgRNA表达盒的碱基序列如下所述。需要说明的是，接下来，在烟草转化及转化体的栽培中从3-3.没有变更点，因此省略说明。
[化学式8]
LS_1A
[化学式9]
LS_3A
[化学式10]
Bl_4A
在上述3个序列中，下划线部分表示引导序列，其上游表示AtU3B启动子序列，其下游表示scaffold-polyT序列，末端小写字母表示KpnI、BamHI的限制酶序列。
(b)有无突变及突变序列的确认
如3-3.(c)中记载所述实施了栽培的转化体中有无突变及突变序列的确认。设计了对位于基因组DNA上的、包含引导序列的区域特异性扩增的引物。将各引物的序列示于以下。另外，如以下所示，仅变更了一部分PCR条件实施。
(2A-1_14、2A-1_19、2A-1_119、2A-1_120、2A_133_17、2A_133_122、2A_133_142：T基因组)
NtBl1-1_2A_F1:AAGTATTACTACTACAAAATTCCAACG、及
Nb_Bl1_2A_R1:CCATCTGATGAAGAACAACTTGC的组合
(2A-1_14、2A-1_19、2A-1_119、2A-1_120、2A_133_17、2A_133_122、2A_133_142：S基因组)
NtBl1-2_1A_F1:TTAAACACTAGAGAGTGAGAGAGTGC、及NtBl1-2_2A_F1:CAGATGTTTAATTATTAAGACAAAGTTCC的组合
(2G-35_10、2G-37_103、2G-126_10、2G-126_139：T基因组)
NtBl1-1_2G_F1:ATATGTTTGAATATAGGGGGAGGG、及NtBl1-1_2G_R1:TGGTTTACAAAAGGAAAAGTTTTC
(2G-35_10、2G-37_103、2G-126_10、2G-126_139：S基因组)
NtBl1-2_2G_F1:ATATGTTTGAGTATAAAGGGAGGA、及NtBl1-2_2G_R1:TTGGTTTACTAGAGAAAAAATTTCC的组合
(LS_1A-8_4、13、LS_1A-9_32、48：T基因组)
LS_1A_F_T:TACCGGTACTGGAAATGACCTC、及LS_1A_R_T:TCCTTAACATTTCGCGGTCT的组合
(LS_1A-8_4、13、LS_1A-9_32、48：S基因组)
LS_1A_F_S:CCGGTACTGGAAATGACCTTG、及LS_1,2_R3:GCAAAGTTGCTTCCAATGAAT的组合
(LS_3A-12、LS_3A-15、及LS_3A-30：T基因组)
LS_3A_4G_F_T:GTTTGGTTCGGAAGAGAAATTATAG、及LS_3A_4G_R_T:CTTTGTCCTTCACCATGCAG的组合
(LS_3A-12、LS_3A-15、及LS_3A-30：S基因组)
LS_3A_4G_F_S:TTGGTTCGGGAGAGAAATAATTGA、及LS_3A_4G_R_S:CGCCAAGAAGATATGGAAAA的组合
(Bl_4A-11、Bl_4A-13、Bl4A-16：T基因组)
Bl_3A_4A_F_T:ATTTCTTCTGCCCACCAGC、及Bl_3A_4A_R_ST:TCTCATCATTGAACACGAACA的组合
(Bl_4A-11、Bl_4A-13、Bl4A-16：S基因组)
Bl_3A_4A_F_S:CCTAATTTGGGTGCTACAAATAAT、及Bl_3A_4A_R_ST:TCTCATCATTGAACACGAACA的组合。
(变更的一部分PCR条件)
LS_1A的T基因组
94℃下60秒钟
以98℃下10秒钟、57℃下15秒钟及68℃下50秒钟作为1个循环，40个循环
LS_1A的S基因组
94℃下60秒钟
以98℃下10秒钟、65℃下15秒钟及68℃下50秒钟作为1个循环，45个循环
LS_3A的T基因组
94℃下60秒钟
以98℃下10秒钟、55℃下15秒钟及68℃下35秒钟作为1个循环，45个循环
LS_3A的S基因组
94℃下60秒钟
以98℃下10秒钟、60℃下15秒钟及68℃下50秒钟作为1个循环，40个循环
Bl_4A的T基因组、S基因组
94℃下60秒钟
以98℃下10秒钟、60℃下15秒钟及68℃下50秒钟作为1个循环，40个循环。
(c)重组体的挑选
按照3-3.中的记载挑选了以下的T2品系及T1品系。首先，将得到的T2品系的个体中的突变体多肽示于以下。
2A-1_14
T基因组：WT由336个氨基酸构成，与此相对，生成在至107个氨基酸为止与WT相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号84)。
S基因组：WT由337个氨基酸构成，与此相对，生成与WT相同的多肽、以及与WT在至106个氨基酸为止相同的氨基酸的基础上添加了无关的3个氨基酸(LEY)的多肽(序列号83)。
2A-1_19
T基因组：WT由336个氨基酸构成，与此相对，生成与WT相同的多肽、以及与WT在至107个氨基酸为止相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号86)。
S基因组：WT由337个氨基酸构成，与此相对，生成在至106个氨基酸为止与WT相同的氨基酸的基础上添加了无关的3个氨基酸(LEY)的多肽(序列号85)。
2A_133_17
T基因组：WT由336个氨基酸构成，与此相对，生成在至107个氨基酸为止与WT相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号98)。
S基因组：由与WT相同的337个氨基酸构成的多肽(序列号97)。
2A_133_122
T基因组：WT由336个氨基酸构成，与此相对，生成在至107个氨基酸为止与WT相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号100)。
S基因组：WT由337个氨基酸构成，与此相对，生成与WT相同的多肽、以及由WT的第107号天冬酰胺缺失的336个氨基酸构成的多肽(序列号99)。
2A_133_142
T基因组：WT由336个氨基酸构成，与此相对，生成在至107个氨基酸为止与WT相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号102)。
S基因组：WT由337个氨基酸构成，与此相对，生成与WT相同的多肽、以及由WT的第107号天冬酰胺缺失的336个氨基酸构成的多肽(序列号101)。
2A_1_119
T基因组：WT由336个氨基酸构成，与此相对，生成在至107个氨基酸为止与WT相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号88)。
S基因组：由与WT相同的337个氨基酸构成的多肽(序列号87)。
2A_1_120
T基因组：WT由336个氨基酸构成，与此相对，生成在至107个氨基酸为止与WT相同的氨基酸的基础上添加了无关的12个氨基酸(TGILNSRKSLWD)的多肽(序列号90)。
S基因组：由与WT相同的337个氨基酸构成的多肽(序列号89)。
2G-35_10
T基因组：WT由336个氨基酸构成，与此相对，生成在至56个氨基酸为止相同的氨基酸的基础上添加了无关的8个氨基酸(KMAKLSKA)的多肽(序列号110)。
S基因组：WT由337个氨基酸构成，与此相对，生成由至56个氨基酸为止相同的氨基酸构成的多肽(序列号109)。
2G-37_103
T基因组：WT由336个氨基酸构成，与此相对，生成由第55和56号这2个氨基酸缺失的334个氨基酸构成的多肽(序列号112)。
S基因组：WT由337个氨基酸构成，与此相对，生成在至54个氨基酸为止相同的氨基酸的基础上添加了无关的7氨基酸(MAKLFKA)的多肽(序列号111)。
2G-126_10
T基因组：WT由336个氨基酸构成，与此相对，生成在至56个氨基酸为止相同的氨基酸的基础上添加了无关的2个氨基酸(DG)的多肽(序列号114)。
S基因组：WT由337个氨基酸构成，与此相对，生成由至56个氨基酸相同的氨基酸构成的多肽(序列号113)。
2G-126_139
T基因组：WT由336个氨基酸构成，与此相对，生成在至54个氨基酸为止相同的氨基酸的基础上添加了无关的1个氨基酸(I)的多肽(序列号118)。
S基因组：WT由337个氨基酸构成，与此相对，生成在至52个氨基酸为止相同的氨基酸的基础上添加了无关的6个氨基酸(AKLFKA)的多肽(序列号117)。
接着，得到的T1品系的个体中的突变体多肽如下所述。
LS_1A-8
T基因组：WT由410个氨基酸构成，与此相对，生成在至247个氨基酸为止与WT相同的氨基酸的基础上添加了无关的15氨基酸(TQALKRPRNVKDFFA)的多肽(序列号73)。
S基因组：WT由407个氨基酸构成，与此相对，生成在至243个氨基酸为止与WT相同的氨基酸的基础上添加了无关的15个氨基酸(PQALERPRKVKDFFA)的多肽(序列号72)。
LS_1A-9
T基因组：WT由410个氨基酸构成，与此相对，生成在至246个氨基酸为止与WT相同的氨基酸的基础上添加了无关的3氨基酸(TGS)的多肽(序列号76)。
S基因组：WT由407个氨基酸构成，与此相对，生成在至244个氨基酸为止与WT相同的氨基酸的基础上添加了无关的15个氨基酸(SQALERPRKVKDFFA)的多肽(序列号75)、以及在至244个氨基酸为止相同的氨基酸的基础上添加了无关的15个氨基酸(TQALERPRKVKDFFA)的多肽(序列号74)。
LS_3A-12
T基因组：WT由410个氨基酸构成，与此相对，生成在至394个氨基酸为止与WT相同的氨基酸的基础上添加了无关的18个氨基酸(SWVGKINPFFPYLLGVKL)的多肽(序列号78)。
S基因组：WT由407个氨基酸构成，与此相对，生成在至391个氨基酸为止与WT相同的氨基酸的基础上添加了无关的66个氨基酸(SWVGKINPFFPYLLGVKFKTLKNKIFIYLHGEGQRGLQSQVLFFFFYIYILFGFKVIGLMNVLILT)的多肽(序列号77)。
LS_3A-15
T基因组：WT由410个氨基酸构成，与此相对，生成在至394个氨基酸为止与WT相同的氨基酸的基础上添加了无关的18个氨基酸(SWVGKINPFFPYLLGVKL)的多肽(序列号80)。
S基因组：WT由407个氨基酸构成，与此相对，生成在至391个氨基酸为止与WT相同的氨基酸的基础上添加了无关的66个氨基酸(SWVGKINPFFPYLLGVKFKTLKNKIFIYLHGEGQRGLQSQVLFFFFYIYILFGFKVIGLMNVLILT)的多肽(序列号79)。
LS_3A-30
T基因组：WT由410个氨基酸构成，与此相对，生成在至397个氨基酸为止与WT相同的氨基酸的基础上添加了无关的14个氨基酸(LAKSTPFFHIFLAL)的多肽(序列号82)。
S基因组：WT由407个氨基酸构成，与此相对，生成在至394个氨基酸为止与WT相同的氨基酸的基础上添加了无关的18个氨基酸(LAKSTPFFHIFLALNLKP)的多肽(序列号81)。
Bl_4A-11
T基因组：WT由336个氨基酸构成，与此相对，生成在至301个氨基酸为止与WT相同的氨基酸的基础上添加了无关的29个氨基酸(MATMAVLLDVTTTTVCSCSMMRIITSQMR)的多肽(序列号121)。
S基因组：WT由337个氨基酸构成，与此相对，生成在至302为止与WT相同的氨基酸的基础上添加了无关的5个氨基酸(QWQQW)的多肽(序列号120)、以及在相同的至302为止与WT相同的氨基酸的基础上添加了无关的4个氨基酸(YYWM)的多肽(序列号119)。
(e)温室中的腋芽发育的评价
在温室中栽培了Bl1基因的至少1个等位基因中不具有突变的7个T2品系的个体(2A-1_14、2A-133_122、2A-133_142、2A-1_19、2A-133_17、2A-1_119及2A-1_120)，按照3-3.的记载对于腋芽进行了评价。2A-1_14、2A-133_122及2A-133_142在S基因组中的Bl1一方的等位基因中不具有突变(T+/+S+/-)。2A-1_19在T基因组中的Bl1一方的等位基因中不具有突变(T+/-S+/+)。2A-133_17、2A-1_119及2A-1_120在S基因组中的Bl1两者的等位基因中不具有突变(T+/+S-/-)。与作为对照的WT(SR-1)相比，评价的7个品系均在一级腋芽的数量、重量方面没有观察到差异，但在二级腋芽的数量、重量方面均显示出显著减少(图22)。因此可知，在二级腋芽的抑制中，不需要在位于S及T基因组上的NtBl1基因的全部等位基因(4个)中导入突变，只要在位于S或T基因组上的NtBl1基因的2个等位基因中导入突变即可。
对于腋芽，评价了5个T2品系(2G-35_10、2G-37_103、2G-126_10、2G-126_139、Bl_4A-11)。这些品系在不同的基因(LS或Bl1)中导入了突变，但该突变在产生移码突变、产生比野生型多肽更短的多肽这一点上是共通的。将对2G-35_10、2G-37_103、2G-126_10、2G-126_139进行评价的结果示于图23，将对Bl_4A-11进行评价的结果示于图24。需要说明的是，图23及24中总结了在打顶后实施的3次腋芽评价。对于在Bl1基因中导入了突变的2G的4个品系、Bl_4A的1个品系而言，在一级腋芽的数量、重量方面没有观察到差异，但在二级腋芽的数量、重量方面显示出显著减少。特别是对于2G的4个品系而言，完全未观察到二级腋芽的发育。根据以上的结果可知，对于Bl1基因而言，在多个不同的突变体中，选择性地抑制了二级腋芽的发育。
接着，同样地对5个T1品系(LS_1A-8、LS_1A-9、LS_3A-12、LS_3A-15、LS_3A-30)进行了评价。将对LS_1A-8、LS_1A-9进行评价的结果示于图25。将对LS_3A-12、LS_3A-15、LS_3A-30进行评价的结果示于图26。需要说明的是，图25及26中总结了在打顶后实施的3次腋芽评价。LS_1A的2个品系、LS_3A的3个品系均在一级腋芽的数量、重量方面没有观察到差异，但完全未观察到二级腋芽的发育。根据以上的结果，对于LS基因而言，与REV基因一样，不仅是从通过EMS处理制备的TUM中挑选出的突变体，而且通过CRISPR/Cas9system制备的多个不同的突变体也选择性地抑制了二级腋芽的发育。
〔参考文献〕
1.Takahashi H,Kamakura H,Sato Y,Shiono K,Abiko T,Tsutsumi N,NagamuraY,Nishizawa NK,Nakazono M.(2010)A method for obtaining high quality RNA fromparaffin sections of plant tissues by laser microdissection.J Plant Res 123:807-813
2.Li JF,Norville JE,Aach J,McCormack M,Zhang D,Bush J,Church GM,SheenJ.(2013)Multiplex and homologous recombination-mediated genome editing inArabidopsis and Nicotiana benthamiana using guide RNA and Cas9.NatBiotechnol.31(8),688-91.
3.Waibel F,Filipowicz W.(1990)U6 snRNA genes of Arabidopsis aretranscribedby RNA polymerase III but contain the same two upstream promoterelements as RNA polymerase II-transcribed U-snRNA genes.Nucleic Acids Res.25；18(12),3451-8.4.Marshallsay C1,Kiss T,Filipowicz W.(1990)Amplification ofplant U3 and U6 snRNA gene sequences using primers specific for an upstreampromoter element and conserved intragenic regions.Nucleic Acids Res.25；18(12),3459-66。
工业实用性
根据本发明，由于可以抑制烟草植物在栽培时不需要的腋芽的发育，因此能够减少栽培的劳动及成本、并且使收获的叶的品质提高。另外，根据本发明，可以选择性地抑制二级腋芽的发育，因此能够提高避免因灾害等引起的损伤所导致的植物体死亡的可能性。
序列表
<110> 日本烟草产业株式会社(JAPAN TOBACCO INC.)
<120> 烟草植物体及其制备方法
<130> JT16269
<150> JP 2016-069742
<151> 2016-03-30
<160> 129
<170> PatentIn version 3.5
<210> 1
<211> 838
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 1
Met Ala Met Val Val Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
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Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Ala Cys Gly Leu Val Ser Leu Glu Pro Thr Lys Ile Ala
210 215 220
Glu Ile Leu Lys Asp Arg Pro Ser Trp Phe Arg Asp Cys Arg Asn Val
225 230 235 240
Glu Val Phe Thr Met Phe Ser Ala Gly Asn Gly Thr Ile Glu Leu Leu
245 250 255
Tyr Thr Gln Ile Tyr Ala Pro Thr Thr Leu Ala Pro Ala Arg Asp Phe
260 265 270
Trp Thr Leu Arg Tyr Thr Thr Thr Leu Glu Asn Gly Ser Phe Val Val
275 280 285
Cys Glu Arg Ser Leu Ser Gly Thr Gly Ala Gly Pro Asn Ala Ala Ser
290 295 300
Ala Ser Gln Phe Val Arg Ala Gln Met Leu Pro Ser Gly Tyr Leu Ile
305 310 315 320
Arg Pro Cys Asp Gly Gly Gly Ser Ile Ile His Ile Val Asp His Leu
325 330 335
Asn Leu Glu Ala Trp Ser Ala Pro Glu Ile Leu Arg Pro Leu Tyr Glu
340 345 350
Ser Ser Lys Val Val Ala Gln Lys Met Thr Ile Ala Ala Leu Arg Tyr
355 360 365
Ala Arg Gln Ile Ala Gln Glu Thr Ser Gly Glu Val Val Tyr Gly Leu
370 375 380
Gly Arg Gln Pro Ala Val Leu Arg Thr Phe Ser Gln Arg Leu Ser Arg
385 390 395 400
Gly Phe Asn Asp Ala Ile Asn Gly Phe Ser Asp Asp Gly Trp Ser Leu
405 410 415
Leu Ser Ser Asp Gly Gly Glu Asp Val Ile Val Ala Val Asn Ser Arg
420 425 430
Lys Asn Ile Ala Thr Thr Ser Val Pro Leu Ser Pro Leu Gly Gly Ile
435 440 445
Leu Cys Ala Lys Ala Ser Met Leu Leu Gln Asn Val Pro Pro Val Val
450 455 460
Leu Val Arg Phe Leu Arg Glu His Arg Ser Glu Trp Ala Asp Phe Asn
465 470 475 480
Val Asp Ala Tyr Val Ala Ser Ser Met Lys Ser Cys Ser Tyr Ala Tyr
485 490 495
Pro Gly Met Arg Pro Thr Arg Phe Thr Gly Ser Gln Ile Ile Met Pro
500 505 510
Leu Gly His Thr Ile Glu His Glu Glu Met Leu Glu Val Ile Arg Leu
515 520 525
Glu Gly His Ser Ile Gly Gln Glu Asp Thr Phe Met Pro Arg Asp Val
530 535 540
His Leu Leu Gln Met Cys Ser Gly Thr Asp Glu Asn Ala Val Gly Ala
545 550 555 560
Cys Ser Glu Leu Val Phe Ala Ala Ile Asp Glu Met Phe Pro Asp Asp
565 570 575
Ala Pro Leu Leu Pro Ser Gly Phe Arg Ile Ile Pro Leu Glu Ser Lys
580 585 590
Ser Ser Asp Pro Gln Asp Thr Ser Asn Ala His Arg Thr Leu Asp Leu
595 600 605
Ala Ser Ser Leu Glu Val Gly Pro Ala Thr Asn Pro Ala Thr Gly Asp
610 615 620
Val Val Ser Gly Tyr Ser Ala Arg Ser Val Leu Thr Ile Ala Phe Gln
625 630 635 640
Phe Pro Phe Glu Asp Asn Leu Gln Asp Asn Val Ala Thr Met Ala Arg
645 650 655
Gln Tyr Val Arg Ser Val Val Ser Ser Val Gln Arg Val Ala Met Ala
660 665 670
Ile Ser Pro Ala Gly Val Asn Ser Thr Phe Gly Ser Lys Leu Ser Pro
675 680 685
Gly Ser Pro Glu Ala Val Thr Leu Ser His Trp Ile Cys Gln Ser Tyr
690 695 700
Ser Tyr His Met Gly Thr Glu Leu Leu Gln Thr Asp Ser Arg Gly Asp
705 710 715 720
Glu Ser Val Leu Lys Asn Leu Trp Gln His Gln Asp Ala Ile Leu Cys
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Cys Ser Leu Lys Ser Leu Pro Val Phe Ile Phe Ala Asn Lys Ala Gly
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Leu Asp Met Leu Glu Thr Thr Leu Val Ala Leu Gln Asp Ile Thr Leu
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Asp Lys Ile Phe Asp Glu Ser Gly Arg Lys Val Leu Phe Ala Glu Phe
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Pro Lys Ile Met Glu Gln Gly Phe Ala Tyr Leu Pro Gly Gly Ile Cys
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Met Ser Ala Met Gly Arg His Ile Ser Tyr Glu Gln Ala Ile Ala Trp
805 810 815
Lys Val Phe Ala Ser Glu Glu Thr Val His Cys Leu Ala Phe Ser Phe
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Ile Asn Trp Ser Phe Val
835
<210> 2
<211> 3882
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 2
gaaagcttta actcaagcaa attctctctc tctctctctc tctctctctc tctctctctc 60
tcttcatttt ctttttctct tttctcaccc accactctca cacacctctt cacctcacct 120
tacacactaa aaaaacatca ctcctctctc taaaaaattc aatctttttg ctgttccaac 180
atgtctttta gagtttgttt cagtttcaga tcttaagggc gggagtgtta tgcttcttct 240
aatattttga agctcaagaa aacagagcaa atttttgctt tcttttctcc tactttttgt 300
ggggggtaat tcttgttttt gtaatctcaa agctggctgt tttatgtata tactgaaggg 360
gttgtggtga tttgtttgtc tactttaaga aggtgccatc tttttcagta atatttgggt 420
aaaagttctc tcttttttgg ccttaaacgc gaagattcag gcctctctca acgtgtcatc 480
tgttctctgt attaaacaca gctggagaat taattacata gaggtaaaaa aaggggttaa 540
agtcgccaaa gaattgaaaa aaaacagagg gctgaggtaa aaagttgatg gtttttaaaa 600
aaaaataaaa gcttaaatga tgataaagtt tggagcttta tgtgaatgga aatggtgttg 660
tgtttgtatc aaacacgagt agtttacagc ttatgtgaat ttgaaagaga gagaattttt 720
gtctgtattt atatcctttt cagccatatc tttcgttaga gcagttttgg ctgtacctta 780
atttgtaagg gtttaagcgt gaagtgtgtg tttgagcctt ctgttataag gggcacaaag 840
tatagaaaca acaaaagggg cacctaggaa tcttctggct caatcaagat cgttcattta 900
atcttgtctg agatcactag aaaaagaaaa aggaaagata aagataaagt ctttgtttca 960
gagaatctta gttctctgtg ttgatatata taataaaagc tgtttgcagg gaatatatct 1020
acttgggggt gtttttattt cttttaaggg tgtttgaaaa tttggaaatc ttgattattt 1080
ttttgtttgg gattttgggg tttgacggca aatggctatg gtggtacagc agcataggga 1140
gagtagtagt ggtagtatta caaaacatct tgacagtagt ggaaagtatg tccggtatac 1200
agctgagcaa gtggaggcat tagagagggt ttatgcagag tgccctaaac ctagctcgtt 1260
gcgccgccag caattgatcc gcgaatgccc tattctgtcg aatatcgagc ctaagcagat 1320
caaagtttgg tttcaaaaca gaaggtgtcg agagaagcaa aggaaagagt cttctagact 1380
acagactgta aatagaaagc tgtctgcaat gaataaacta ttaatggagg agaatgatcg 1440
cttgcaaaaa caggtttcac agcttgtgtg tgaaaatggc tttatgcggc aacaattgca 1500
tactgcatca gcggccacta ctgatgtaag ttgtgagtca gtggttacca cccctcagca 1560
ttccctcaga gatgctaaca accctgctgg actgctgtcg attgcagagg aaaccttagc 1620
agagttcctt tccaaggcta caggaactgc tgttgattgg gtcccgatgc ctgggatgaa 1680
gcctggtccg gattcagttg ggatttttgc catctcacac agttgtagtg gagtggcagc 1740
ccgagcatgt ggtcttgtta gtttagagcc gacaaagatt gctgagatcc tcaaagatcg 1800
accatcttgg ttccgagact gccggaacgt tgaagttttc acgatgtttt ctgcaggaaa 1860
tggaacaatt gagcttttgt acacgcagat atatgctcct accaccttgg ctcctgcacg 1920
tgatttttgg actctgagat acacaaccac cctggagaat ggtagttttg tggtttgtga 1980
aagatccctc tctggtactg gagctgggcc gaatgctgct tctgcttccc agtttgtaag 2040
agctcaaatg cttccgtccg gatatctaat ccgaccgtgt gacggtggag gatccattat 2100
acatattgtt gaccatctga atcttgaggc atggagtgcc cctgagattt tgcgtccact 2160
ttatgaatcg tcaaaagttg tggcacagaa aatgactatt gcggcactgc gatatgcaag 2220
gcaaatagct caggagacta gtggggaggt tgtatatggt ctgggaaggc aacctgcagt 2280
tcttcgaaca tttagccaga gattaagcag aggcttcaat gacgccatca atggattcag 2340
tgatgatggc tggtcattgt taagttctga tggtggtgaa gatgttatag ttgctgtcaa 2400
ttcaaggaag aacattgcca ccacttccgt tcctctttca ccgctgggag gcatcctttg 2460
tgccaaagca tcaatgctac tccagaatgt tcctcctgtg gtactggttc gatttctcag 2520
ggagcaccgt tcagagtggg cggactttaa tgttgatgcc tatgtagctt cgtcaatgaa 2580
atcttgttca tatgcatatc ctgggatgag gcctaccaga tttaccggaa gtcagataat 2640
aatgccactt ggccatacaa ttgaacatga agagatgctt gaggttatta gattggaagg 2700
acactctatt ggccaggaag atacttttat gccaagagat gttcaccttc tccagatgtg 2760
tagtggaact gatgagaatg ctgtcggagc ttgttctgaa ctagtttttg ctgcaattga 2820
tgagatgttt ccagatgatg cacccctgtt gccctccggg tttcgtatca ttcctctcga 2880
gtcaaaatca agcgatcccc aggatacatc gaatgctcat agaacactgg atctggcatc 2940
aagtcttgaa gttggcccag caacaaaccc tgctactgga gatgtggtct ctggctacag 3000
tgcacgatct gtgttgacaa ttgcttttca atttccattc gaggacaatc ttcaggacaa 3060
tgtagctacc atggcgcgcc agtatgttcg cagtgtggtt tcatctgtcc aacgggttgc 3120
catggcaata tctcccgcag gagtgaattc aacattcggg tccaagcttt ctccaggctc 3180
ccctgaagct gtaacgttgt cgcactggat ctgccagagc tacagttatc acatggggac 3240
agagttgctt caaactgatt cgaggggcga tgaatcagtg ctaaaaaatc tttggcaaca 3300
tcaggatgct attttgtgct gctcattgaa gtccctgccg gttttcattt ttgctaataa 3360
ggctgggctt gatatgctgg agacaacctt agttgcttta caggacatta ctctagataa 3420
gatatttgat gaatctggcc ggaaagtgtt gttcgctgaa tttcccaaga tcatggaaca 3480
gggttttgcg tacttgccgg gtggtatttg catgtcagca atgggacgac atatttcata 3540
tgaacaagct attgcatgga aagtctttgc ttctgaagaa actgtccact gcttagcctt 3600
ctcatttatt aactggtcat ttgtttaatg ttgctgtcaa atctcctttc ttttttttcc 3660
tttttgtttt ttgacatctt cctcacagag gacactgaca gccaggaaca cagttgaacg 3720
gaatgatctt tgggacggat gaaaattttg taacttgggg ggctcccgtc tgttttacct 3780
ttaatttaat tagactaaat ttgtattttg cttcctgaat tcttcatact cttatgtaaa 3840
ttttctagtg cagctttttt gagtgcagat gtttgtttcc gc 3882
<210> 3
<211> 839
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 3
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Ala Cys Gly Leu Val Ser Leu Glu Pro Thr Lys Ile Ala
210 215 220
Glu Ile Leu Lys Asp Arg Ser Ser Trp Phe Arg Asp Cys Arg Asn Val
225 230 235 240
Glu Val Phe Thr Met Phe Ser Ala Gly Asn Gly Thr Ile Glu Leu Leu
245 250 255
Tyr Thr Gln Ile Tyr Ala Pro Thr Thr Leu Ala Pro Ala Arg Asp Phe
260 265 270
Trp Thr Leu Arg Tyr Thr Thr Thr Leu Glu Asn Gly Ser Phe Val Val
275 280 285
Cys Glu Arg Ser Leu Ser Gly Thr Gly Ala Gly Pro Asn Ala Ala Ser
290 295 300
Ala Ser Gln Phe Val Arg Ala Gln Met Leu Pro Ser Gly Tyr Leu Ile
305 310 315 320
Arg Pro Cys Asp Gly Gly Gly Ser Ile Ile His Ile Val Asp His Leu
325 330 335
Asn Leu Glu Ala Trp Ser Ala Pro Glu Ile Leu Arg Pro Leu Tyr Glu
340 345 350
Ser Ser Lys Val Val Ala Gln Lys Met Thr Ile Ala Ala Leu Arg Tyr
355 360 365
Ala Arg Gln Ile Ala Gln Glu Thr Ser Gly Glu Val Val Tyr Gly Leu
370 375 380
Gly Arg Gln Pro Ala Val Leu Arg Thr Phe Ser Gln Arg Leu Ser Arg
385 390 395 400
Gly Phe Asn Asp Ala Ile Asn Gly Phe Ser Asp Asp Gly Trp Ser Leu
405 410 415
Leu Ser Ser Asp Gly Gly Glu Asp Val Ile Val Ala Val Asn Ser Arg
420 425 430
Lys Asn Ile Ala Thr Thr Ser Val Pro Leu Ser Pro Leu Gly Gly Ile
435 440 445
Leu Cys Ala Lys Ala Ser Met Leu Leu Gln Asn Val Pro Pro Ala Val
450 455 460
Leu Val Arg Phe Leu Arg Glu His Arg Ser Glu Trp Ala Asp Phe Asn
465 470 475 480
Val Asp Ala Tyr Val Ala Ser Ser Met Lys Ser Cys Ser Tyr Ala Tyr
485 490 495
Pro Gly Val Arg Pro Thr Arg Phe Thr Gly Ser Gln Ile Ile Met Pro
500 505 510
Leu Gly His Thr Ile Glu His Glu Glu Met Leu Glu Val Ile Arg Leu
515 520 525
Glu Gly His Ser Ile Gly Gln Glu Asp Ala Phe Met Pro Arg Asp Ile
530 535 540
His Leu Leu Gln Met Cys Ser Gly Thr Asp Glu Asn Ala Val Gly Ala
545 550 555 560
Cys Ser Glu Leu Val Phe Ala Ala Ile Asp Glu Met Phe Pro Asp Asp
565 570 575
Ala Pro Leu Leu Pro Ser Gly Phe Arg Ile Ile Pro Leu Glu Ser Lys
580 585 590
Ser Ser Asp Pro Gln Asp Thr Ser Asn Ala His Arg Thr Leu Asp Leu
595 600 605
Ala Ser Ser Leu Glu Val Gly Pro Ala Thr Asn Pro Ala Thr Gly Asp
610 615 620
Val Val Ser Gly Tyr Ser Ala Arg Ser Val Leu Thr Ile Ala Phe Gln
625 630 635 640
Phe Pro Phe Glu Asp Asn Leu Gln Asp Asn Val Ala Thr Met Ala Arg
645 650 655
Gln Tyr Val Arg Ser Val Val Ser Ser Val Gln Arg Val Ala Met Ala
660 665 670
Ile Ser Pro Ala Gly Val Asn Ser Thr Phe Gly Ser Lys Leu Ser Pro
675 680 685
Gly Ser Pro Glu Ala Val Thr Leu Ser His Trp Ile Cys Gln Ser Tyr
690 695 700
Ser Tyr His Met Gly Thr Glu Leu Leu Gln Ala Asp Ser Arg Gly Asp
705 710 715 720
Glu Ser Val Leu Lys Asn Leu Trp Gln His Gln Asp Ala Ile Leu Cys
725 730 735
Cys Ser Leu Lys Ser Leu Pro Val Phe Ile Phe Ala Asn Lys Ala Gly
740 745 750
Leu Asp Met Leu Glu Thr Thr Leu Val Ala Leu Gln Asp Ile Thr Leu
755 760 765
Asp Arg Ile Phe Asp Glu Ser Gly Arg Lys Val Leu Phe Ala Glu Phe
770 775 780
Pro Lys Ile Met Asp Gln Gly Phe Ala Tyr Leu Pro Gly Gly Ile Cys
785 790 795 800
Met Ser Ala Met Gly Arg His Ile Ser Tyr Glu Gln Ala Ile Ala Trp
805 810 815
Lys Val Phe Ala Ser Glu Glu Thr Ser Val His Cys Leu Ala Phe Ser
820 825 830
Phe Ile Asn Trp Ser Phe Val
835
<210> 4
<211> 2884
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 4
aagctgtttg cagggaatat atctacttgg gggtgttttt atttcttaaa agggtgtttg 60
aaaatttgga aatcttgatt ttttttttgg tttgggattt tgaggtttga gggcaatggc 120
tatggttgca cagcagcaca gggagagtag tagtggtagt attacaaaac atcttgacag 180
tagtggaaag tatgtccggt atacagctga gcaagttgag gcattggaga gggtttatgc 240
tgagtgccct aagcctagct ccttgcgccg ccaacaattg atccgtgaat gccctattct 300
gtcgaatatc gagcctaagc agatcaaagt ttggtttcaa aacagaaggt gtcgagagaa 360
gcaaaggaaa gagtcttctc gactacagac tgtaaataga aagctgtctg caatgaataa 420
actattgatg gaggagaatg atcgcttgca aaaacaggtt tcgcagcttg tgtgtgaaaa 480
tggctttatg cggcaacagt tgcatactgc atcagcggcc actactgatg taagttgtga 540
gtctgtggta actacccctc agcattccct cagagatgct aacaaccctg ctggactgct 600
gtcgattgca gaggaaacct tagcagagtt cctttccaag gctacaggaa ctgctgttga 660
ttgggtcccg atgcctggga tgaagcctgg tccggattca gttgggattt ttgccatctc 720
acacagttgt agtggagtgg cagcccgagc atgtggtctt gttagtttag agccgacaaa 780
gattgctgag atcctcaaag atcgatcttc ttggttccga gattgccgga acgttgaagt 840
tttcacaatg ttttctgcag gaaatggaac aattgaactt ttgtacacgc agatatatgc 900
tcctaccacc ttggctcctg cacgtgattt ttggactctg agatacacaa ccaccctgga 960
gaatggtagc tttgtggttt gtgaaagatc cctctctggt actggagctg ggccgaatgc 1020
tgcttctgct tcccagtttg taagagctca aatgcttccg tctggatatc taatccgacc 1080
gtgtgacggt ggaggatcca ttatacatat tgttgaccac ctgaatcttg aggcatggag 1140
tgcccctgag attttgcgtc cactttatga atcgtcaaaa gttgtggcac agaaaatgac 1200
tattgcggca ctgcgatatg caaggcaaat agctcaggag actagtgggg aggttgtata 1260
tggtctggga aggcaacctg cagttcttcg aacatttagc cagagattaa gcagaggctt 1320
caatgatgcc atcaatggat tcagtgatga tggctggtca ttgttaagtt ctgatggtgg 1380
tgaagatgtt atagttgctg tcaattcaag gaagaacatt gccaccactt ccgttcctct 1440
ttcaccactt ggaggcatcc tttgtgccaa agcatcaatg ctactccaga atgttcctcc 1500
tgcggtactg gttcgatttc tcagggagca ccgttcagag tgggcggact ttaatgttga 1560
tgcctatgta gcttcctcaa tgaaatcttg ttcatatgca tatcctgggg tgaggcctac 1620
cagatttacc ggaagccaga taataatgcc actgggccac acaatagaac atgaagagat 1680
gcttgaagtt attagattgg aagggcactc tattggccag gaagatgctt ttatgccgag 1740
agatattcac cttctccaga tgtgtagtgg aaccgatgag aatgctgtcg gagcttgttc 1800
tgaactagtt tttgctgcaa ttgatgagat gtttccagat gatgcacccc tgttgccctc 1860
cgggtttcgt atcattcctc tcgagtcaaa atcaagcgat ccccaggata catcgaatgc 1920
tcatagaaca ctggatctgg catcaagtct tgaagttggc ccagcaacaa accctgctac 1980
tggagatgtg gtctctggct acagtgcacg atctgtattg acaattgctt ttcaatttcc 2040
attcgaggac aatcttcagg ataatgtagc taccatggcg cgccagtatg ttcgcagtgt 2100
ggtttcatct gtccaacggg ttgccatggc aatatctccc gcaggagtga attcaacatt 2160
cgggtccaag ctttctccag gctcccctga agctgtaact ttgtcgcact ggatctgcca 2220
gagctacagt tatcacatgg ggacagagtt gcttcaagct gattcgaggg gcgatgaatc 2280
agtgctaaag aatctttggc aacatcagga tgctattttg tgctgctcat tgaagtcgct 2340
gccggttttc atttttgcta ataaggctgg gcttgatatg ctggagacaa cattagttgc 2400
tttgcaagac attactctag ataggatatt tgacgaatct ggccggaaag tgttgttcgc 2460
tgaatttccc aagatcatgg atcagggttt cgcgtacctg ccgggtggta tttgcatgtc 2520
tgcaatggga cgacatattt catatgaaca agctattgca tggaaagtct ttgcttctga 2580
agaaactagt gtccactgct tagccttctc atttattaac tggtcatttg tttaatgttg 2640
ctgtcaaatc tcctcttttt tttccttttt gttttttgac atcttcctca cagaggacac 2700
tgacagacag gaacacagtt gaacggaaag atcttgggac cgatgaaaat ttttgtaact 2760
tgtggggctc ctgtctgttt tgccttaatt taattagact aaatttgtat tttgcttccc 2820
ggattcttca tactcttgtg taaatttact agtgcagctt ttttgagtgc agatgtttgt 2880
ttcc 2884
<210> 5
<211> 407
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 5
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Lys Leu Arg Ile
245 250 255
Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val Thr Leu Ala
260 265 270
Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln Arg Phe Val
275 280 285
Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu Glu Ala Thr
290 295 300
Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln Val Trp Phe
305 310 315 320
Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp Lys Arg Arg
325 330 335
Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu Arg Ser Cys
340 345 350
Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser Gln Ala Lys
355 360 365
Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln Leu Ser Val
370 375 380
Ser Ser Thr Ser Asn Ser Phe Phe Leu Gly Trp Gln Asn Gln Pro Leu
385 390 395 400
Phe Ser Ile Ser Ser Trp Arg
405
<210> 6
<211> 1623
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 6
atttcccctc ctccatcatt gaaaaccccc tctgtccttt cccctagaga gacccctttt 60
tcctctctct ctcctttctc tttttattag acgcatatat tctctcttct ttctctttct 120
agggttttca cctgaaatag ttttatttcg gtgatatgtt aggatccttt ggttcatcat 180
ctcaatctca tgatgaagaa actgatgatc aacggcggag attcagttcc acttcccctg 240
caatccaaat ccggcaacta ctcattagct gcgcggagtt aatctcgcgg tccgatttct 300
cggccgcaaa cagactcctc accattttat caactaactc ttcccctttt ggtgattcaa 360
ctgaaagatt agtccatcag ttcactcgcg cactttctct tcgcctcaac cgttatatct 420
cttcagccac taatttcttg acaccatcta atgttgttga aagttcaaat gattcagctc 480
tacttcagtc atcctatctt tccctaaacc aagtgactcc tttcattaga tttagtcagc 540
taactgctaa tcaagcgatt ttggaagcta ttaacgataa ccaacaagcg atccacatcg 600
ttgattttga tattaatcac ggtgttcaat ggccaccgtt aatgcaagca ctagctgatc 660
gttaccctcc tccaactctt cggattaccg gtactggaaa tgaccttgat acccttcgta 720
gaaccggaga tcgtttagct aaatttgctc actctttagg ccttagattt cagtttcacc 780
ctcttttgat taccaataat aatgacaatg atcatgaccc ttcaataatt tcttctattg 840
ttcttctccc tgatgagaca ttagctatca actgtgtatt ttatcttcac aggctcttga 900
aagaccgcga aaagttaagg atttttttgc ataggattaa atccatgaac cctaaagttg 960
taacgctggc cgagagagaa gcaaatcata atcacccact ttttttgcaa agatttgtgg 1020
aggctttgga ttattatgca gctgtgtttg attcattgga agcaactttg ccaccgagca 1080
gtagagagag gatgacagtg gaacaagttt ggttcgggag agaaataatt gatatagtag 1140
cagcagaagg agataagaga agagaaagac acgagagatt cagatcatgg gaagtaatgt 1200
tgaggagctg tggatttagc aatgttgctt taagcccttt tgcactctca caagctaaac 1260
ttctcttgag acttcattac ccatctgaag gataccagct tagtgtttcg agtacgagta 1320
attctttctt cttgggttgg caaaatcaac cccttttttc catatcttct tggcgttaaa 1380
tttaaaaccc taaaaaacaa gatttttatc tatctgcatg gtgaaggaca aagaggtctt 1440
caatctcagg ttcttttttt tttttttttt ttatatatat atcttgtttg ggtttaaggt 1500
tattgggctg atgaatgttt taattttaac ataggtctac ttacgtagta gttataggtt 1560
gataatgaga tataattaac taagtctttg tataatgcag atcctgaact taatctttat 1620
ttg 1623
<210> 7
<211> 410
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 7
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Met
245 250 255
Leu Arg Ile Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val
260 265 270
Thr Leu Ala Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln
275 280 285
Arg Phe Val Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu
290 295 300
Glu Ala Thr Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln
305 310 315 320
Val Trp Phe Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp
325 330 335
Lys Arg Arg Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu
340 345 350
Arg Ser Cys Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser
355 360 365
Gln Ala Lys Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln
370 375 380
Leu Ser Val Ser Ser Thr Ser Asn Ser Phe Phe Leu Gly Trp Gln Asn
385 390 395 400
Gln Pro Leu Phe Ser Ile Ser Ser Trp Arg
405 410
<210> 8
<211> 1827
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 8
aggttcttct tccttaatat tgagtcaaga ttagtactac tactatagcc aagaaaatgt 60
gaaatcatat agtactaact ttcccttctc cctagctact gataactcta attaatttca 120
gatgccaaaa ccataaattt cccctcctcc atcattgaaa acccctttgt cctttccccc 180
cagaccccct tttcctctct ctctctctcc tttctctttt tattagacgc atattctctc 240
ttctttctct ttctagggtt ttcacctgaa atagttttat ttcgttgata tgttaggatc 300
ctttggttca tcatctcaat ctcatgatga agaagctgat gatcaacggc ggagatgcag 360
ttccacttcc cctgcaatcc aaatccggca actactcatt agctgcgcgg agttaatctc 420
acggtccgat ttctcggcgg caaacagact cctcaccatt ttatcaacta actcttcccc 480
ttttggtgat tcaactgaaa gattagtcca tcagttcact cgcgcacttt ccattcgcct 540
caaccgctat atctcttcag ccactaattt cttgacacct aatgcatcat ctaatgttgt 600
tgaaagttca aatgattcag ctctacttca gtcatcctat ctttccctaa accaagtgac 660
cccttttatt agatttagtc agctaactgc taatcaagcg attttagaag ctattaacga 720
taaccaacaa gcgatccaca tcgttgattt tgatattaat cacggtgttc aatggccacc 780
gttaatgcaa gcactagctg atcgttaccc tcctccaact cttcggatta ccggtactgg 840
aaatgacctc gatacccttc gtagaaccgg agatcgttta gctaaatttg ctcactcttt 900
aggccttaga tttcagtttc accctctttt gatcaccaat aataatgaca atgatcatga 960
cccttcaatc atttcttcta ttgttcttct ccctgatgag acattagcaa tcaactgtgt 1020
attttatctt cacaggctct taaaagaccg cgaaatgtta aggatttttt tgcataggat 1080
taaatccatg aaccctaaag ttgtaacact ggccgagaga gaagcaaatc ataatcaccc 1140
actttttttg caaagatttg tggaggcttt ggattattat gcagctgtct ttgattcatt 1200
ggaagcaact ttgccgccga gcagtagaga gaggatgaca gtggagcaag tttggttcgg 1260
aagagaaatt atagatatag tagcagcaga aggagataag agaagagaaa gacacgagag 1320
attcagatca tgggaagtaa tgttgaggag ctgtggattt agcaatgttg ctttaagtcc 1380
ttttgcactt tcacaagcta aacttctctt gagacttcat tacccttctg aaggatacca 1440
gcttagtgtt tcgagtacga gtaattcttt cttcttgggt tggcaaaatc aacccctttt 1500
ttccatatct tcttggcgtt aaattataag ggaaattaaa accctaaaaa caagatttta 1560
tctatctgca tggtgaagga caaagaggtc ttcaatctca ggttcttttt gtttttttaa 1620
cttgtttgga tatgaggtta ttgagctgat gaatgtttta attttaacat aggcctactt 1680
acgtagtagt tataggttga taatgatata tatttaacta agtctttgta taatgcagat 1740
cctgaactta atttttattt ttattatttt gttgttaatg aaagattctg ttaccaaatt 1800
ttatcagtct atttaattag aggccaa 1827
<210> 9
<211> 337
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 9
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro
145 150 155 160
Asn Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr
165 170 175
Asn Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala
180 185 190
Thr Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met
195 200 205
Phe Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met
210 215 220
Ser Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu
225 230 235 240
Gln Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln
245 250 255
Asn Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln
260 265 270
Lys Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly
290 295 300
Asn Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val
305 310 315 320
Phe Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Glu Met Phe Tyr
325 330 335
Tyr
<210> 10
<211> 1239
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 10
gtccatctgt ctatataggt agaatgagag taaaggagaa aacatatcct cctctccatt 60
tctgtagaca aagattctca aagagaaaca aattaaacac tagagagtga gagagtgcta 120
taagaaaaag aatatgggga gagctccatg ttgtgataaa gcaaatgtga agagagggcc 180
atggtctcct gaagaagatg ctaaactcaa agatttcatt cacaaatatg gaactggtgg 240
aaattggatt gctcttcctc aaaaagctgg actaaagaga tgtgggaaga gttgtagatt 300
gagatggcta aattatttaa ggcctaacat taaacatggt gatttttctg aggaagaaga 360
tagagttatt tgcaccttgt attccaccat tggaagcagg tggtcaataa tagcagctca 420
attaccggga agaactgaca atgatatcaa gaattactgg aatactaagc tcaagaaaaa 480
acctatggga ttaatgcaat caactaacca aagaaaatca ccatattttc cagctactaa 540
ttctcttcaa acccaacccc agataaattc aagtcttttt agagacttat attacacccc 600
aaataatagg cctaatatta caggcctaaa tcatcagtcc atttcttctg cccaccagac 660
aaattttctc tacactaata ataacatgaa ctttcctaat ttgggtgcta caaataatca 720
atatccttat aatatccaaa gtcataattt acttatgttt ggagaagcaa gttgttcttc 780
atcagatgga agttgcagcc aaatgagttt tggtaaagaa atcaagagag aagaaattat 840
gagtaatagt ttacaacaag gtcaaatttc aagtgttaat gcttttgaag aaaaccacca 900
gaattttact cttgattatg gcaatagtag tagtaattgg gtggatcaaa aaccaaatgt 960
gtattttggt actactacta ctcaagtact tcagtatgat aatgttgaag aagttaagca 1020
gcagctaaca agttgtacca atggcaacaa tggtagtact attggatgta acaacaacaa 1080
cagtatgttc gtgttcaatg atgagaatta taacaagtca aatgagatag agatgttcta 1140
ttactaaaga agaaatgact gttgaaaaga aaacaaatgc aagtaccatt aggaagattt 1200
gaaagggcgt ttgggtatgg gggttgccaa gaagattca 1239
<210> 11
<211> 336
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 11
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Ala Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Asn Pro Asn Asn Arg Pro
145 150 155 160
Ile Ile Thr Gly Leu Asn Gln Ser Ile Ser Ser Ala His Gln Pro Asn
165 170 175
Phe Leu Tyr Thr Asn Ser Asn Met Asn Phe Pro Asn Leu Gly Ala Thr
180 185 190
Asn Ser Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met Phe
195 200 205
Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met Ser
210 215 220
Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Cys Leu Gln
225 230 235 240
Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn Gln Asn Phe
245 250 255
Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln Lys Pro
260 265 270
Asn Val Tyr Phe Gly Asn Thr Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly Asn
290 295 300
Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val Phe
305 310 315 320
Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Gly Met Phe Tyr Tyr
325 330 335
<210> 12
<211> 1343
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 12
gtccacttgt ctatatagca agaaagagag taaaggagaa aacatattct cctctccatt 60
tctgtagaca agattctcaa aaagaaacaa attaaacact agagagtgag agagaactat 120
aagaaaaaga atatggggag agctccatgt tgtgataaag caaatgtgaa gagagggcca 180
tggtctcctg aagaagatgc taaactcaaa gatttcattc acaaatatgg aactggtgga 240
aattggattg ctcttcccca aaaagcagga ctaaagagat gtgggaagag ttgtagattg 300
agatggctaa attatctaag gcctaatatc aaacatggtg atttttcgga ggaagaagat 360
agagttattt gcagcttgta ttccaccatt ggaagcaggt ggtcaataat agcagctcaa 420
ttaccaggaa ggactgacaa tgatatcaag aattactgga atactaaact caagaaaaag 480
cttatgggat taatgcaatc aacaaaccaa agaaaatcac catattttcc agctactaat 540
tctcttcaag cccaacccca gataaattca agtcttttta gagacttata ttacaaccca 600
aataataggc ctattattac aggcctaaat cagtccattt cttctgccca ccagccaaat 660
tttctctaca ctaatagtaa catgaatttt cctaatttgg gtgctacaaa tagtcaatat 720
ccttataata ttcaaagtca taatttactt atgtttggag aagcaagttg ttcttcatca 780
gatggaagtt gtagccaaat gagttttggc aaagaaatca agagagagga aattatgagt 840
aattgtttac aacaaggtca aatttcaagt gttaatgctt ttgaagaaaa tcagaatttc 900
actcttgatt atggtaacag tagtagtaat tgggtggatc aaaaaccaaa tgtgtatttt 960
ggaaatacta ctactactac tcaagtactt cagtatgatg ttgaagaagt taagcagcag 1020
ctaacaagtt gtaccaatgg caacaatggc agtactattg gatgtaacaa caacaacagt 1080
atgttcgtgt tcaatgatga gaattataac aagtcaaatg agatagggat gttctattac 1140
tgaagaagaa atgactagct gttgaaaaga gaaaacaaat gtaagtacac cattaggaag 1200
atttgaaagg gcgtttgggt atgggggttg gcaagaagat tcaaactttt tctggggttt 1260
tgtgtaattg tggtggaatt attattattg aaacttcttt acttcaattt aaatcgtcgg 1320
tacatattac gtagttgtag tac 1343
<210> 13
<211> 168
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 13
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Arg Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Arg Arg His Arg Ile Ser Asp Arg
35 40 45
Phe Lys Ile Leu Gln Ser Leu Ile Pro Gly Gly Ser Lys Met Asp Thr
50 55 60
Val Thr Met Leu Glu Glu Ala Ile His Tyr Val Lys Phe Leu Lys Thr
65 70 75 80
Gln Ile Trp Leu His Gln Thr Val Ile Asn Ile Val Asp Asp Tyr Asp
85 90 95
Asn Pro Asn Tyr His Asp Gln Leu Leu Met Ala His Asp Ser Asn Phe
100 105 110
Ala Asn Tyr Tyr Pro His Glu Met Val Glu Tyr Cys Pro Ala Pro Val
115 120 125
Glu Asn Ala Gln Ile Asn Tyr Asn Leu Asp Gln Leu Gln Leu Pro Gly
130 135 140
Tyr Ala Phe Ser Asp Gly Asp Gln Phe Gln Gly Glu Glu Thr Asn Ile
145 150 155 160
Thr Gly Asp Ser Phe Met Tyr Tyr
165
<210> 14
<211> 594
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 14
aaaatagagg taattagttg tatcaatgga tcaacaacat tccacttgtt tttcttcttc 60
aagtaaaatt aatgacaaag aaaagaagaa aaaaagatca gttgtgaaac tatcaactga 120
tccacaaagt gtagcagctc gtgaaagaag gcatagaatc agtgatcgtt tcaagatttt 180
gcagagttta atccctggtg gttcaaaaat ggatacagtt actatgttag aagaagcaat 240
tcactatgtc aaatttctta agactcaaat atggctgcat caaaccgtga ttaatattgt 300
agatgattat gataatccaa attatcatga tcagttgcta atggctcatg actctaattt 360
tgctaattat tatcctcatg aaatggtgga atattgccca gctcctgttg agaatgcaca 420
aataaattat aacttggacc agctgcagct tccaggttat gcattttcag atggggatca 480
attccaagga gaagaaacta atattactgg tgattctttt atgtactatt agttagttaa 540
ttatgttgcc taagtttaat tagaatacgt agtgtgtggt agtatggtat gttg 594
<210> 15
<211> 165
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 15
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Gly Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Arg Arg His Arg Ile Ser Asp Arg
35 40 45
Phe Lys Ile Leu Gln Ser Leu Val Pro Gly Gly Ser Lys Met Asp Thr
50 55 60
Val Thr Met Leu Glu Glu Ala Ile His Tyr Val Lys Phe Leu Lys Met
65 70 75 80
Gln Ile Trp Leu His Gln Thr Met Ile Asn Ile Val Asp Asp Tyr Asp
85 90 95
Asn Pro Asn Tyr His His Gln Leu Leu Met Ala His Asp Ser Asn Phe
100 105 110
Ala Asn Tyr Tyr Pro His Glu Asn Asn Ser Thr Pro Val Glu Asn Ala
115 120 125
Gln Ile Asn Tyr Asn Leu Asp Gln Leu Gln Leu Pro Gly Tyr Ala Phe
130 135 140
Ser Asp Gly Asp Gln Phe Gln Gly Glu Glu Thr Asn Ile Ser Gly Asp
145 150 155 160
Ala Phe Met Tyr Tyr
165
<210> 16
<211> 660
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 16
tgcatggaca atctcctctt ctcaaacttc ataaagatat tatattaaaa aaaataaaga 60
agaagagaag atagaggtaa ttagctatag caatggatca acaacattcc acttgttttt 120
cttcttcaag caaaattaat gacaaagaaa agaagaaaaa aggatcagtt gtgaaactat 180
caactgatcc acaaagtgta gcagctcgtg aaagaaggca tagaatcagt gatcgtttca 240
agattttgca gagtttagtc cctggtggtt ctaaaatgga cacagttaca atgttagaag 300
aagcaattca ctatgtcaaa tttctcaaga tgcaaatatg gctgcatcaa accatgatta 360
atattgtaga tgattatgat aatccaaatt atcatcatca gttgctaatg gctcatgact 420
ctaattttgc taattattat cctcatgaga ataactcaac tcctgttgag aatgcacaaa 480
taaattataa cttggaccag ctgcagcttc caggttatgc attttcagat ggagatcaat 540
tccaaggaga agaaactaat atttctggtg atgcttttat gtactattaa ttagtaatta 600
gttaattatg ttgcctaagt ttaattagaa tacgtagtgt gtggtagtat ggtatgttgt 660
<210> 17
<211> 400
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 17
Met Leu Ser Met Glu Glu Ile Leu Cys Glu Leu Ser Arg Glu Asp Met
1 5 10 15
Asn Asn Glu Lys Gly Leu Pro Pro Gly Phe Arg Phe His Pro Thr Asp
20 25 30
Glu Glu Leu Ile Thr Phe Tyr Leu Ala Ser Lys Val Phe Asn Gly Thr
35 40 45
Phe Cys Gly Ile Gln Ile Ala Glu Val Asp Leu Asn Arg Cys Glu Pro
50 55 60
Trp Glu Leu Pro Glu Val Ala Lys Met Gly Glu Arg Glu Trp Tyr Phe
65 70 75 80
Phe Ser Leu Arg Asp Arg Lys Tyr Pro Thr Gly Leu Arg Thr Asn Arg
85 90 95
Ala Thr Gly Ala Gly Tyr Trp Lys Ala Thr Gly Lys Asp Arg Glu Val
100 105 110
Tyr Ser Ala Thr Asn Gly Ala Leu Leu Gly Met Lys Lys Thr Leu Val
115 120 125
Phe Tyr Lys Gly Arg Ala Pro Lys Gly Glu Lys Thr Lys Trp Val Met
130 135 140
His Glu Tyr Arg Leu Asp Gly Asp Phe Ser Tyr Arg Tyr Ser Ser Lys
145 150 155 160
Glu Glu Trp Val Ile Cys Arg Ile Leu His Lys Ile Gly Glu Lys Lys
165 170 175
Asn Pro Ile Tyr Gln Ala Ala Gly Gln Asn Tyr Gly Tyr Pro Thr Ser
180 185 190
Leu Lys Thr Trp Pro Ser Ser Ser Phe Leu Asn Thr Ala Thr Ser Ala
195 200 205
Glu Ala Ala Pro Asn Pro Ile Leu Ala Glu Thr Pro Asn Pro Lys Thr
210 215 220
Thr Thr Thr Thr His Trp Gln Glu Ser Phe Gln Ile Ser Gln Asn Ser
225 230 235 240
Met Gln Ser Leu His Asn Phe Tyr Leu Phe His His Gln Glu Asn Asp
245 250 255
Leu Met Lys Ser Leu Phe Asn Pro Ile Asn Val Ser Gln Thr Asn Leu
260 265 270
Phe Pro Ile Asn Asn Ser Val Leu Ser Ser Ala Thr Ser Phe Ser Thr
275 280 285
Ser Gln Ser Thr Lys Lys Tyr Lys Glu Asp Ile Asn Lys Asn Ser Ser
290 295 300
Leu Ser Ser Phe Leu Val Ser Asn Ser Lys Lys Asn Glu Lys His Gln
305 310 315 320
Val Pro Leu Met Gln Ala Asn Thr Thr Met Lys Thr Glu Ala Ser Phe
325 330 335
Ser Pro Tyr Ser Gly Cys Tyr Asn Asp Gln Asn Pro Met Ala Thr Asn
340 345 350
Phe Gly Met Asn Asn Ser Thr Asp Trp Ser Leu Val Gly Ile Glu Gly
355 360 365
Met His Phe Asn Gly Gly Cys Thr Gln Ser Gln Met Val Leu Asp His
370 375 380
Met His Cys Pro Ile Lys Ile Ala Ala Glu Ser Trp Pro Leu Asp Leu
385 390 395 400
<210> 18
<211> 1461
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 18
tactatcact taataccatc attcatcatg ttgtcaatgg aagaaatatt gtgtgaacta 60
agtagagaag acatgaataa tgagaaaggt ctaccacctg gttttaggtt tcatcctact 120
gatgaagagc ttatcacttt ctaccttgcc tctaaggttt ttaacggcac cttttgtggt 180
attcagattg ctgaagttga tctcaacaga tgtgagccct gggaacttcc agaagtggca 240
aagatggggg aaagagaatg gtatttcttt agcttaaggg acagaaaata cccaaccgga 300
ctaagaacaa accgggcaac aggagctggt tattggaaag ctacaggaaa agatagagaa 360
gtgtacagtg caacaaatgg agcactcctt gggatgaaga aaacattggt tttttacaaa 420
ggaagagcac caaagggtga gaaaaccaaa tgggttatgc atgaatatcg tcttgacggc 480
gatttttcct accgttactc ttctaaggag gaatgggtga tatgcagaat actacacaaa 540
ataggggaga agaaaaatcc aatataccaa gctgcaggac aaaactatgg ctaccctaca 600
agcttgaaaa catggccatc atcatctttt cttaacacag caacatcagc agaagcagct 660
ccaaatccta tattggctga aacaccaaat ccaaaaacca caacaactac acattggcaa 720
gaatcattcc aaatatcaca aaactcaatg caatcactgc acaactttta tctatttcac 780
caccaagaaa acgaccttat gaaatccctc ttcaacccca ttaatgtttc ccaaacaaac 840
ctcttcccaa taaataatag tgtcctttct tctgctacct ccttttctac atcccaaagc 900
acaaaaaaat acaaagaaga cataaacaaa aactcgtcac tatcatcttt cctcgttagc 960
aattcaaaga aaaatgaaaa acatcaagtc ccactcatgc aggctaacac aacaatgaaa 1020
acagaagcca gtttttcacc atattctggt tgttacaatg atcaaaaccc tatggctacg 1080
aattttggta tgaataattc aacagattgg agtttagtag gcatagaagg gatgcatttt 1140
aatggtggat gtactcagtc tcagatggtg ttggatcaca tgcattgtcc catcaaaata 1200
gctgcagaat cttggcctct tgatctctaa aaatagaaga ttttgttttt aataaattct 1260
actgtaggat gatatggtaa ttaattatta ctcctgttat atcatcctta tctatgaata 1320
gctatcctct aagtatataa aagtaattca ggctgctctt tatattctga aacatgttgc 1380
ttgctcctta agcatctagg taataccgct atgtaagata tatcttcctt ttctcgacta 1440
aagttgtgat ctattgatgg g 1461
<210> 19
<211> 399
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 19
Met Leu Ser Met Glu Glu Ile Leu Cys Glu Leu Ser Arg Asp Asp Met
1 5 10 15
Asn Asn Glu Lys Gly Leu Pro Pro Gly Phe Arg Phe His Pro Thr Asp
20 25 30
Glu Glu Leu Ile Thr Phe Tyr Leu Ala Ser Lys Val Phe Asn Gly Thr
35 40 45
Phe Cys Gly Ile Gln Ile Ala Glu Val Asp Leu Asn Arg Cys Glu Pro
50 55 60
Trp Glu Leu Pro Glu Val Ala Lys Met Gly Glu Arg Glu Trp Tyr Phe
65 70 75 80
Phe Ser Leu Arg Asp Arg Lys Tyr Pro Thr Gly Leu Arg Thr Asn Arg
85 90 95
Ala Thr Gly Ala Gly Tyr Trp Lys Ala Thr Gly Lys Asp Arg Glu Val
100 105 110
Tyr Ser Ala Thr Asn Gly Ala Leu Leu Gly Met Lys Lys Thr Leu Val
115 120 125
Phe Tyr Lys Gly Arg Ala Pro Lys Gly Glu Lys Thr Lys Trp Val Met
130 135 140
His Glu Tyr Arg Leu Asp Gly Asp Phe Ser Tyr Arg Tyr Ser Ser Lys
145 150 155 160
Glu Glu Trp Val Ile Cys Arg Ile Leu His Lys Ile Gly Glu Lys Lys
165 170 175
Asn Pro Ile Tyr Gln Ala Ala Gly Gln Asn Tyr Gly Tyr Pro Thr Ser
180 185 190
Leu Lys Thr Trp Pro Ser Ser Ser Phe Leu Asn Thr Ala Ala Pro Asn
195 200 205
Pro Ile Leu Ala Glu Thr Pro Asn Pro Lys Thr Thr Thr Thr Thr His
210 215 220
Trp Gln Glu Ser Phe Gln Ile Ser Gln Asn Ser Val Gln Ser Leu His
225 230 235 240
Asn Leu Tyr Leu Phe His His Gln Glu Asn Asp Leu Met Lys Ser Leu
245 250 255
Phe Ser Pro Ile Asn Val Ser Gln Thr Asn Leu Phe Pro Ile Asn Asn
260 265 270
Ser Asp Leu Ser Ser Ala Ala Ser Phe Ser Thr Ser Gln Ser Thr Lys
275 280 285
Lys Tyr Lys Glu Asp Ile Asn Lys Asn Ser Ser Ile Ser Ser Phe Leu
290 295 300
Phe Ser Asn Ser Phe Cys Thr Ser Lys Lys Asn Glu Lys Gln Gln Val
305 310 315 320
Pro Leu Met Gln Ala Asn Thr Thr Met Lys Thr Glu Ala Ser Phe Ser
325 330 335
Pro Tyr Ser Gly Cys Tyr Asn Asp Gln Asn Pro Met Ala Ser Thr Phe
340 345 350
Gly Met Asn Asn Ser Ser Asp Trp Ser Leu Val Gly Ile Glu Gly Met
355 360 365
His Phe Asn Gly Gly Cys Thr Gln Ser Gln Met Val Leu Asp His Met
370 375 380
Asn Cys Pro Ile Lys Ile Thr Ala Glu Ser Trp Pro Leu Asp Leu
385 390 395
<210> 20
<211> 1465
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 20
ctactacatc acttaatatc attcattatg ttgtcaatgg aagaaatatt gtgtgaactt 60
agtagagatg acatgaataa tgagaaaggt ctaccacctg gttttaggtt tcatcctact 120
gatgaagagc ttatcacttt ctaccttgcc tctaaggttt ttaacggcac cttttgtggt 180
attcagattg ctgaagttga cctcaacaga tgtgagccct gggaacttcc agaagtggca 240
aagatggggg agagagaatg gtatttcttt agcttaaggg acagaaaata cccaaccggg 300
ctaagaacaa accgggcaac aggagcaggt tattggaaag ctacaggaaa agatagggaa 360
gtgtacagtg caaccaatgg agcactcctt gggatgaaga aaacactggt tttttacaaa 420
ggaagagcac caaagggtga gaaaaccaag tgggttatgc acgaatatcg tcttgacggt 480
gatttttctt accgctactc ttctaaggag gaatgggtga tatgcagaat actacacaaa 540
ataggggaga agaaaaatcc aatataccaa gctgcaggac aaaactatgg ctaccctaca 600
agcttgaaaa catggccatc atcatctttt ctcaacacag cagctccaaa tcccatattg 660
gctgaaacac caaatccaaa aaccacaact actacacatt ggcaagaatc attccaaata 720
tcacaaaact cagtgcaatc actgcacaac ctttatctat ttcaccacca agaaaacgac 780
cttatgaaat ccctcttcag tcccattaat gtttcccaaa caaacctctt cccaataaat 840
aatagtgacc tttcttctgc tgcctccttt tctacatccc aaagcaccaa aaaatacaaa 900
gaagacataa acaaaaactc gtcaatatca tctttcctct ttagcaattc cttttgcact 960
tcaaagaaaa atgaaaaaca gcaagttcca ctaatgcagg ctaacacaac aatgaaaaca 1020
gaagctagtt tttcaccata ttctggttgt tacaatgatc aaaaccctat ggcttcgact 1080
tttgggatga ataattcatc agattggagt ttagtaggca tagaagggat gcattttaat 1140
ggtggatgta ctcagtctca gatggtgttg gatcacatga attgtcccat caaaatcact 1200
gcagaatctt ggcctctcga tctctaaaaa tagaagagtt gtttttccat aatttctata 1260
gtaggatgat atggtaatta attatgacta ctgttatgtc atcctctata tatagctatc 1320
cgctctagta tatgtaatct ttgtaattaa tttaggctgc tttattctga aagatgttgc 1380
tttctcctta aggatatatc tagctagtac cgctatgtaa gatatatctt tcttttctcg 1440
actaatgtaa agttgcaatc tattg 1465
<210> 21
<211> 7032
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 21
tctcaaagct ggctgtttta tgtatatact gaaggggttg tggtgatttg tttgtctact 60
ttaagaaggt gccatctttt tcagtaatat ttgggtaaaa gttctctctt ttttggcctt 120
aaacgcgaag attcaggcct ctctcaacgt gtcatctgtt ctctgtatta aacacagctg 180
gagaattaat tacatagagg taaaaaaagg ggttaaagtc gccaaagaat tgaaaaaaaa 240
cagagggctg aggtaaaaag ttgatggttt ttaaaaaaaa ataaaagctt aaatgatgat 300
aaagtttgga gctttatgtg aatggaaatg gtgttgtgtt tgtatcaaac acgagtagtt 360
tacagcttat gtgaatttga aagagagaga atttttgtct gtatttatat ccttttcagc 420
catatctttc gttagagcag ttttggctgt accttaattt gtaagggttt aagcgtgaag 480
tgtgtgtttg agccttctgt tataaggggc acaaagtata gaaacaacaa aaggggcacc 540
taggaatctt ctggctcaat caagatcgtt catttaatct tgtctgagat cactagaaaa 600
agaaaaagga aagataaaga taaagtcttt gtttcagaga atcttagttc tctgtgttga 660
tatatataat aaaagctgtt tgcagggaat atatctactt gggggtgttt ttatttcttt 720
taagggtgtt tgaaaatttg gaaatcttga ttattttttt gtttgggatt ttggggtttg 780
agggcaaatg gctatggtgg tacagcagca tagggagagt agtagtggta gtattacaaa 840
acatcttgac agtagtggaa agtatgtccg gtatacagct gagcaagtgg aggcattaga 900
gagggtttat gcagagtgcc ctaaacctag ctcgttgcgc cgccagcaat tgatccgcga 960
atgccctatt ctgtcgaata tcgagcctaa gcagatcaaa gtttggtttc aaaacagaag 1020
gtacactgcc cgctgttcaa ttttgattgc tccaatttgg tttctttttt gttcttaaat 1080
gcatatattt aggtgtcgtg cacttgtgat cttggactga aatatgggat aagttagatg 1140
agtgatggtt aaattggaat atatcactgt gcttctagtt tcctaggctt gtcgattggg 1200
ttgtatggat taatcggggg gggggggcat taagtgaatc gtgaattgga tgtgtagttt 1260
gatttctgtc tgtcgggtag ttgagcttag attttggaat tgagggtgaa cattgtgcca 1320
tttcaggtgt cgagagaagc aaaggaaaga gtcttctaga ctacagactg taaatagaaa 1380
gctgtctgca atgaataaac tattaatgga ggagaatgat cgcttgcaaa aacaggtttc 1440
acagcttgtg tgtgaaaatg gctttatgcg gcaacaattg catactgtaa gttaacataa 1500
tttttccttt atacttgtgg taaaaagctt tattttttgc ttactgtaga cgaatggtaa 1560
cgtatatctt gtcttttgtt tctgatgaaa tggctaagca ctatgaattt taagatttct 1620
gatattccac agcttatggt aacatatttt aaacagtgta aataaacttt attctgatga 1680
cactgtttta ggacattctt atagttatgg aatgcatggc tttagatatg ggactaaatt 1740
ttatgttcat cgtgtttttg cattctatat tcttctactc gcccttgttt tgctgtgaag 1800
ttgaaccaat aaacaagaaa cagatgatgg atatctccgg tgatcttttg ttccatagga 1860
ttaattagac tgtatttgtg ttttctgcag gcatcagcgg ccactactga tgtaagttgt 1920
gagtcagtgg ttaccacccc tcagcattcc ctcagagatg ctaacaaccc tgctgggtaa 1980
ttaatttcaa acacctattt ctcccatcct ttccgtctat ggtgtccatt ctccaacata 2040
tttatgttat ttattcaatg gcatatacaa cattttgagg ggctaatttg tttatctcta 2100
agtcaagttt gttctctatg cagactgctg tcgattgcag aggaaacctt agcagagttc 2160
ctttccaagg ctacaggaac tgctgttgat tgggtcccga tgcctgggat gaaggtttga 2220
actttagtca atcctcttta ttttttgaaa attcagtatt gccatgtctc tttgactgga 2280
tagctaaaaa actaaatttt cattctattg ccagcctggt ccggattcag ttgggatttt 2340
tgccatctca cacagttgta gtggagtggc agcccgagca tgtggtcttg ttagtttaga 2400
gccgacaaag gtaagcagtc atgtggaaaa ttaatttaaa tgtagtgctg ttgctctatt 2460
actagttttg gtcctttgac gggtgtacta gatgttgcca gtttcttctt agtaaatata 2520
tttttgtcaa atatttacag attgctgaga tcctcaaaga tcgaccatct tggttccgag 2580
actgccggaa cgttgaagtt ttcacgatgt tttctgcagg aaatggaaca attgagcttt 2640
tgtacacgca ggtaattaat taccttctca tcaatcttca cgtaggcttc tgattggaga 2700
agctacagca ttgaggggat ttttgaaatc atttcttttc agatatatgc tcctaccacc 2760
ttggctcctg cacgtgattt ttggactctg agatacacaa ccaccctgga gaatggtagt 2820
tttgtggtat gcacatcctc cgcattagcg tgtcttagga taagcaatct ggccactttt 2880
gtacttagtt atgaatattt tgctgatagt ttgttgtatg tgccatcaat tcctcctccc 2940
ctcaaggttt gtgaaagatc cctctctggt actggagctg ggccgaatgc tgcttctgct 3000
tcccagtttg taagagctca aatgcttccg tccggatatc taatccgacc gtgtgacggt 3060
ggaggatcca ttatacatat tgttgaccat ctgaatcttg aggtcagatt gcacactgta 3120
ctaccacttc cctttctttt taacttgttc tgtcttgcag ctggacttca cggcataatg 3180
tttttcttca ggcatggagt gcccctgaga ttttgcgtcc actttatgaa tcgtcaaaag 3240
ttgtggcaca gaaaatgact attgcggtga gttgaaccgt tgattgtcat taaatactgg 3300
atgtgtaaca acctttttag tcttcacaac tagatctcaa tttttgttga gctctgaagt 3360
cgaaagggtt gtaatttctg gacgagcagt tagatatagc ctgatatttt tgtttattca 3420
gttagaagtt cccagcttta aaaatataga acacctgaca aatccttagt ctcttaatgc 3480
acgttattga ggatttcttt gttttttcga gttttctaag gttcattatt gttttcctca 3540
tggggttgcc ataaaagtct gcatgtgaaa catatagtat tgaagaactg taggctgtga 3600
agcgcaccat actcttaact gcattagttg ttgctttaat tccatatgtt gctctgagaa 3660
tacttgcagc attttttatg tttcaagtac ttgagcaatt accgtagctt accatcacaa 3720
caaaagaaat actaattata gtatgttttt gctgtaaagg cactgcgata tgcaaggcaa 3780
atagctcagg agactagtgg ggaggttgta tatggtctgg gaaggcaacc tgcagttctt 3840
cgaacattta gccagagatt aagcaggtgc tgtttattgc tctgattgtt ctgtgctatg 3900
agatatgata tgccataaaa gtagacatac gaattctgaa gcacaagtat cataattaag 3960
ctattttcta tattgcagag gcttcaatga cgccatcaat ggattcagtg atgatggctg 4020
gtcattgtta agttctgatg gtggtgaaga tgttatagtt gctgtcaatt caaggaagaa 4080
cattgccacc acttccgttc ctctttcacc gctgggaggc atcctttgtg ccaaagcatc 4140
aatgctactc caggtgaata gattaccttt taactgacta gaaattttca ttggccaact 4200
acctttgcct tgttagataa aattgttcca gactgttgca gattttgatg atgctttcaa 4260
tttctaaact cttggaatga atcgggattc ctggaatata agagaatatt actcagtgtt 4320
ctataaagct atttgtttaa tgcaccatgt ggggcatctt gttgctatta aatggaagaa 4380
tgagaattga cttttaactc ttctgtatgg tggcagaatg ttcctcctgt ggtactggtt 4440
cgatttctca gggagcaccg ttcagagtgg gcggacttta atgttgatgc ctatgtagct 4500
tcgtcaatga aatcttgttc atatgcatat cctgggatga ggcctaccag atttaccgga 4560
agtcagataa taatgccact tggccataca attgaacatg aagaggtaag cactttgcac 4620
ttgccccagt tccatccatc ccatgtgttg gagtgtgctt atacagcacc agtatttttt 4680
ataatcagaa agttagcact ctttgaattg ctaggcttgt tacctaatat tgctaatatt 4740
atactttaga cttcctctca tttttttttt attttgtttt gctttgcaga tgcttgaggt 4800
tattagattg gaaggacact ctattggcca ggaagatact tttatgccaa gagatgttca 4860
ccttctccag gtaccttttg cctatgcatt gatgtttcgg tgtgttatct acgtacagac 4920
attgttgaag caatagctaa caaacggtta tttctagatg tgtagtggaa ctgatgagaa 4980
tgctgtcgga gcttgttctg aactagtttt tgctgcaatt gatgagatgt ttccagatga 5040
tgcacccctg ttgccctccg ggtttcgtat cattcctctc gagtcaaaat cagttgagta 5100
aaaatatttc attttcaact ttaagcattg aatttggcca atctattgtt tacatggatt 5160
atttttcatt ttgcttgatt ttggagcata accggtgatt ctattttcag agcgatcccc 5220
aggatacatc gaatgctcat agaacactgg atctggcatc aagtcttgaa gttggcccag 5280
caacaaaccc tgctactgga gatgtggtct ctggctacag tgcacgatct gtgttgacaa 5340
ttgcttttca atttccattc gaggacaatc ttcaggacaa tgtagctacc atggcgcgcc 5400
agtatgttcg cagtgtggtt tcatctgtcc aacgggttgc catggcaata tctcccgcag 5460
gagtgaattc aacattcggg tccaagcttt ctccaggctc ccctgaagct gtaacgttgt 5520
cgcactggat ctgccagagc tacaggtaaa atgatttctc aactatggtg aaaccttgtt 5580
ctcttcgttt cagctcaata tggggtttat tgctttacat gttcatactg tcgtgcttac 5640
aagtcactcg ttgcaaatct catttaccac caagagccaa agtagtgtca agtgtgcatg 5700
ttgagatctt caattatttt atgagaattt ttcctttctc aacatattga gaaaaagcag 5760
acggtcttag aagtactttt ctgattgtta acataccgtt ttcttctttt gcatttaata 5820
tccagttatc acatggggac agagttgctt caaactgatt cgaggggcga tgaatcagtg 5880
ctaaaaaatc tttggcaaca tcaggatgct attttgtgct gctcattgaa ggtatgaatt 5940
ctcttatcat gtaaacagca tgttacggtt agtaaaaaaa tattgtatgt tgtgttgcgg 6000
tgaaacatga acatatacgt aaagaaaaaa tgtattaacc tagtaaatcc acgatgaagg 6060
cagatttgtt caaaagttaa tctcatgacc ctaattaata ttagaatacg aaagagctgg 6120
acaaggatat tagaaataag tccgacttaa attatacttg tgatggtgat attttatggt 6180
gaaaatgcca tatcatgggt gtatatttga actacttgtg attggcattt tgattgtcct 6240
cattttggtc cctagcatgc ttttgacatg tcaacatgga aatgagttgc taagaaattg 6300
gaaggactga cctattcgtt cacgttcctt ttatcttgtt aaaagaatgt gtttagtaag 6360
ttaaatttct ttctctgctg ttgcagtccc tgccggtttt catttttgct aataaggctg 6420
ggcttgatat gctggagaca accttagttg ctttacagga cattactcta gataagatat 6480
ttgatgaatc tggccggaaa gtgttgttcg ctgaatttcc caagatcatg gaacaggtat 6540
ttacagctga ctctggtctt ttgcagaacc tagaaaacaa aagttgaggt cttaactgtt 6600
acttttttcc gcgatgttga ttcttgatca tagggttttg cgtacttgcc gggtggtatt 6660
tgcatgtcag caatgggacg acatatttca tatgaacaag ctattgcatg gaaagtcttt 6720
gcttctgaag aaactgtcca ctgcttagcc ttctcattta ttaactggtc atttgtttaa 6780
tgttgctgtc aaatctcctt tctttttttt cctttttgtt ttttgacatc ttcctcacag 6840
aggacactga cagccaggaa cacagttgaa cggaatgatc tttgggacgg atgaaaattt 6900
tgtaacttgg ggggctcccg tctgttttac ctttaattta attagactaa atttgtattt 6960
tgcttcctga attcttcata ctcttatgta aattttctag tgcagctttt ttgagtgcag 7020
atgtttgttt cc 7032
<210> 22
<211> 7155
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 22
tctcaaagct ggctgtttta tgtatatact gaaggggttg tggtgatttg tttgtctact 60
ttaagaaggt gccatctttt tcagtaatat ttgggtaaag gttctctttt tttggcctta 120
cacgcgaaga ttcaggcctc tctgaacgtg tcatttgttc tctgtattaa acacagctgg 180
agaagtaatt acatcaaggt agaaaaaggg gttaaagatt ccaaagaatt gagtgtttga 240
aaaaaaaaac agagggctga ggtaaaaagt tgatggtttt aaaaaaaata aattaaatga 300
tgatagagtt tggagcttta tgtgaatgga aatggtgttg tgtttttatc aaacacgagt 360
agtttacagc ttatgtgaat ttgaaagaga gagagaattt ttgtctgtat ttatatcctt 420
ttcagccata tctttcgtta gagcagtttt ggctgtacct taatttgtaa gtttttaagc 480
gtgaagtgtg tgtttgagcc ttctgttata aggggcacaa agtatagaaa caacaaaagg 540
gggcacctag gaatcttctg gctcaatcaa gatcgttcat ttaatcttgt ctgagatcac 600
tagaaaaaga aaaaaaaaag agataaagat aaagtctttg tttcagagaa tcttagttct 660
ctgtgttgat atatataata aaagctgttt gcagggaata tatctacttg ggggtgtttt 720
tatttcttaa aagggtgttt gaaaatttgg aaatcttgat tttttttttg gtttgggatt 780
ttgaggtttg agggcaatgg ctatggttgc acagcagcac agggagagta gtagtggtag 840
tattacaaaa catcttgaca gtagtggaaa gtatgtccgg tatacagctg agcaagttga 900
ggcattggag agggtttatg ctgagtgccc taagcctagc tccttgcgcc gccaacaatt 960
gatccgtgaa tgccctattc tgtcgaatat cgagcctaag cagatcaaag tttggtttca 1020
aaacagaagg tacactgccc attgttcaat ttggattact ccaatttggt ttcttttttg 1080
ttcttaaatg catatattta ggtgtgtact gcacttgtga tcttgggctc tagtttgttt 1140
ggtactgctc aaatcttgga ttagttagat cagtgatgga tgaagtggaa tatatcactg 1200
tccttctagt ttcctaggct tgtcgattgg gttgtatgag ttaaccgtgg ggcattaagt 1260
gaatcatgaa ttgcatatgt agtttgattt ctgtctgttg ggtagttgag cttagatttt 1320
ggaatagagg gtgaatattg tatcatttca ggtgtcgaga gaagcaaagg aaagagtctt 1380
ctcgactaca gactgtaaat agaaagctgt ctgcaatgaa taaactattg atggaggaga 1440
atgatcgctt gcaaaaacag gtttcgcagc ttgtgtgtga aaatggcttt atgcggcaac 1500
agttgcatac tgtaagttaa cataattttt cctttattat ttatggtaaa aaaccttttt 1560
tttcacttaa cgtatcttgt cttttgtttc tgataagcac tatggatttt aagattcctg 1620
atattccaca gcttatggta acatatttta aacagtgtaa attgtcttta ttttgatgac 1680
aggttttagg tcattcttat agttacgaaa tgcatgacta aattttgaat tcatcgtgtt 1740
tttgctttct atattcttct acccgccctt cttgttttgc tgtgatattg aaccaatgga 1800
caagaaacgg atggcagata tctccggtga tcttttgttc tgtaggaatt aattagactg 1860
tatttgtgtt ttctgcaggc atcagcggcc actactgatg taagttgtga gtctgtggta 1920
actacccctc agcattccct cagagatgct aacaaccctg ctgggtaatt aatttcaaac 1980
tcctatttct cccacccctt ctgtctatgg tgtttataca tatttatgtt atttattaaa 2040
tggcatagac cacattttga ggggctaatt tgtttatctc taagtcaagt ttgttctctc 2100
cgcagactgc tgtcgattgc agaggaaacc ttagcagagt tcctttccaa ggctacagga 2160
actgctgttg attgggtccc gatgcctggg atgaaggttt gaactttagt caatcctttt 2220
ttgttttaaa aaaaaattca gtattgccac gtgcctcttt gactggatag ctaaaaaact 2280
aaattttcat tctattgtca gcctggtccg gattcagttg ggatttttgc catctcacac 2340
agttgtagtg gagtggcagc ccgagcatgt ggtcttgtta gtttagagcc gacaaaggta 2400
agcagtcttg tggaaaatta atttaaatgt agtgctgctg ctctattact agttttggtc 2460
ccttgatgag tgtactagat tatgccagtt tcttctaagt acatatattt ttgtctaata 2520
tttacagatt gctgagatcc tcaaagatcg atcttcttgg ttccgagatt gccggaacgt 2580
tgaagttttc acaatgtttt ctgcaggaaa tggaacaatt gaacttttgt acacgcaggt 2640
aattaattac tttctcatca atcttcacgt aggcttctga ttggagaagc tacagcattg 2700
aggggattgt tgaaatcatt ttttttccag atatatgctc ctaccacctt ggctcctgca 2760
cgtgattttt ggactctgag atacacaacc accctggaga atggtagctt tgtggtaagc 2820
acatccttca cattagtgtg tcttaggatt agcaatctgg ccacttttgt acttagttat 2880
gaatattttg ctgatagttt gttgtatgtg cccatcaatt cctcctcccc gtaaggtttg 2940
tgaaagatcc ctctctggta ctggagctgg gccgaatgct gcttctgctt cccagtttgt 3000
aagagctcaa atgcttccgt ctggatatct aatccgaccg tgtgacggtg gaggatccat 3060
tatacatatt gttgaccacc tgaatcttga ggtcagatta cacgctgtac taccacttct 3120
ctttcttatt agcttgttct gtcttgcagc tggacttcac tgcataatat tgtttttcag 3180
gcatggagtg cccctgagat tttgcgtcca ctttatgaat cgtcaaaagt tgtggcacag 3240
aaaatgacta ttgcggtgag ttgaaccctt ggtttttatt aactactgga tgtttaacaa 3300
cctttttggt cttcacaact agatctcaat ttttgttcag ctctgaagta gataggattg 3360
tactttctgg acgagcagtt agatatagcc tgatattttt gtttattctg ttagaagttc 3420
ccagctttaa aaatatagaa cacctgacaa atccttagtc tcttaatgca cgttatcgag 3480
gatttcttcg ttattcgagt tttcaaaggt tcattattgt tttcctcatt gtgttgccat 3540
aaaagtctgc atgtgaaaca tataagtaat gaagaacctt atgctgtgaa gcacagcata 3600
ctgttaactg cattcgatgt tgcttaattc cagaagttgc tctgagaata cttacagcct 3660
ttttttatat tttaagtact tgagcaatta ccgttactta ccacaacagc aaaagaaata 3720
ctaattatgg ttagtttttg ctgtaaaggc actgcgatat gcaaggcaaa tagctcagga 3780
gactagtggg gaggttgtat atggtctggg aaggcaacct gcagttcttc gaacatttag 3840
ccagagatta agcaggtgct ggttattgct ctgattgttc tgtgcttcga gatatgatat 3900
gccataaaag tagacatacg aatcctgaag cgcaagtatc ataattaggc tattttctat 3960
attgcagagg cttcaatgat gccatcaatg gattcagtga tgatggctgg tcattgttaa 4020
gttctgatgg tggtgaagat gttatagttg ctgtcaattc aaggaagaac attgccacca 4080
cttccgttcc tctttcacca cttggaggca tcctttgtgc caaagcatca atgctactcc 4140
aggtcaacag attaagcttt cttgaactaa ctacagattt tcattggcca actacctttg 4200
ccttgttaat tcactgaata ggtcaagtaa ttctaaagac aagttttgca gtgctcttgt 4260
tgccttgtta gttcatagca aacagagttg cagctgttca aagtaggatc atatattgtg 4320
atacctattc agtatctgta ttagatctag tatcacaaga caagttttct ttactgctct 4380
tgtttcttag aaattggctc tatactctta ctaaaaaaga gcgataatgg tagattttga 4440
agtcgaggaa aaattaaaat cgttccggat tgttgcagat ttttattatg ctttcaattt 4500
ctaattctag gaaagaatca ggattcctgg aatattagag aatattactc agtgttttat 4560
aaagctattt gtttaatgct ctgagtaggg catcttgcta ttaattggaa gaatgagaat 4620
tgacttttaa ctcttttgtt cggtggcaga atgttcctcc tgcggtactg gttcgatttc 4680
tcagggagca ccgttcagag tgggcggact ttaatgttga tgcctatgta gcttcctcaa 4740
tgaaatcttg ttcatatgca tatcctgggg tgaggcctac cagatttacc ggaagccaga 4800
taataatgcc actgggccac acaatagaac atgaagaggt aagcggtttg caattgcccc 4860
agttctcact tatgtgttat ggggaatgcc tcgacataca tgagcaagaa tttgagactt 4920
gagacttcct ctcactttat tttggtttgc agatgcttga agttattaga ttggaagggc 4980
actctattgg ccaggaagat gcttttatgc cgagagatat tcaccttctc caggtacttt 5040
tgcttataca ttgatgtttc ggtgtgttgt atgtacatac attgttgaag gataatgcta 5100
acaaacagtt atttctagat gtgtagtgga accgatgaga atgctgtcgg agcttgttct 5160
gaactagttt ttgctgcaat tgatgagatg tttccagatg atgcacccct gttgccctcc 5220
gggtttcgta tcattcctct cgagtcaaaa tcagttgagt aaaatatttt gattttcaac 5280
ttcaagcatt gaatttggca aatctattgt ttacatggat tttttttttt cttttcattt 5340
tgctcgattt tggagcataa ccggtgattc tattttcaga gcgatcccca ggatacatcg 5400
aatgctcata gaacactgga tctggcatca agtcttgaag ttggcccagc aacaaaccct 5460
gctactggag atgtggtctc tggctacagt gcacgatctg tattgacaat tgcttttcaa 5520
tttccattcg aggacaatct tcaggataat gtagctacca tggcgcgcca gtatgttcgc 5580
agtgtggttt catctgtcca acgggttgcc atggcaatat ctcccgcagg agtgaattca 5640
acattcgggt ccaagctttc tccaggctcc cctgaagctg taactttgtc gcactggatc 5700
tgccagagct acaggtaaaa tgatttctca actatggtga aaccttattc tctgcatttc 5760
agctcaatat ggggtttatt gctttacatg ttcatactgt cgtgcttaca agtcgattca 5820
ttgcaaatct catttaccac caagagcgga agcagtgtcg agtgtgcatg ttgatcttca 5880
attatttttt gagaattttt cctttctcaa catattgaga aaaatcagat ggtcttagaa 5940
gtacttttct gattgttaac ataccgtttt cttcttttgc atataatatc cagttatcac 6000
atggggacag agttgcttca agctgattcg aggggcgatg aatcagtgct aaagaatctt 6060
tggcaacatc aggatgctat tttgtgctgc tcattgaagg tatgaattct cttatgaact 6120
catgtaaaca gcatattacg gtttgttagt aaaaaaattg taggttgtgt tgcggtgaaa 6180
catgaacata tgcataaaga aaaatgtatt aacctagtag tgtcatgacc ctaattaata 6240
ttagaatatg aaggagctgg acaatgatat taagaaataa gctcgactta aattatattt 6300
gtgatggtga ttttttatgg tgaaaatgtc atatcatggg tgcatatttg aactacttgt 6360
gattggcatt ttgattgtcc tcattttggt ccctagcatg cttttgacat gtcaacatgc 6420
attgcttttg acctattcat ccgccttcta gtcttttatc ttgttaaatg aatggcgtta 6480
gtaagttgaa tttctttctc tgctgttgca gtcgctgccg gttttcattt ttgctaataa 6540
ggctgggctt gatatgctgg agacaacatt agttgctttg caagacatta ctctagatag 6600
gatatttgac gaatctggcc ggaaagtgtt gttcgctgaa tttcccaaga tcatggatca 6660
ggtatttaca gccgactctt agtctttgca gaaccgagaa accaaagttg aggtcttaac 6720
tcttactttc ttcgattctg tttattcttg atcatagggt ttcgcgtacc tgccgggtgg 6780
tatttgcatg tctgcaatgg gacgacatat ttcatatgaa caagctattg catggaaagt 6840
ctttgcttct gaagaaacta gtgtccactg cttagccttc tcatttatta actggtcatt 6900
tgtttaatgt tgctgtcaaa tctcctcttt ttttcctttt tgttttttga catcttcctc 6960
acagaggaca ctgacagaca ggaacacagt tgaacggaaa gatcttggga ccgatgaaaa 7020
tttttgtaac ttgtggggct cctgtctgtt ttgccttaat ttaattagac taaatttgta 7080
ttttgcttcc cggattcttc atactcttgt gtaaatttac tagtgcagct tttttgagtg 7140
cagatgtttg tttcc 7155
<210> 23
<211> 1457
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 23
atttcccctc ctccatcatt gaaaacccct ttgtcctttc cccccagacc cccttttcct 60
ctctctctct ctcctttctc tttttattag acgcatattc tctcttcttt ctctttctag 120
ggttttcacc tgaaatagtt ttatttcgtt gatatgttag gatcctttgg ttcatcatct 180
caatctcatg atgaagaagc tgatgatcaa cggcggagat gcagttccac ttcccctgca 240
atccaaatcc ggcaactact cattagctgc gcggagttaa tctcacggtc cgatttctcg 300
gcggcaaaca gactcctcac cattttatca actaactctt ccccttttgg tgattcaact 360
gaaagattag tccatcagtt cactcgcgca ctttccattc gcctcaaccg ctatatctct 420
tcagccacta atttcttgac acctaatgca tcatctaatg ttgttgaaag ttcaaatgat 480
tcagctctac ttcagtcatc ctatctttcc ctaaaccaag tgaccccttt tattagattt 540
agtcagctaa ctgctaatca agcgatttta gaagctatta acgataacca acaagcgatc 600
cacatcgttg attttgatat taatcacggt gttcaatggc caccgttaat gcaagcacta 660
gctgatcgtt accctcctcc aactcttcgg attaccggta ctggaaatga cctcgatacc 720
cttcgtagaa ccggagatcg tttagctaaa tttgctcact ctttaggcct tagatttcag 780
tttcaccctc ttttgatcac caataataat gacaatgatc atgacccttc aatcatttct 840
tctattgttc ttctccctga tgagacatta gcaatcaact gtgtatttta tcttcacagg 900
ctcttaaaag accgcgaaat gttaaggatt tttttgcata ggattaaatc catgaaccct 960
aaagttgtaa cactggccga gagagaagca aatcataatc acccactttt tttgcaaaga 1020
tttgtggagg ctttggatta ttatgcagct gtctttgatt cattggaagc aactttgccg 1080
ccgagcagta gagagaggat gacagtggag caagtttggt tcggaagaga aattatagat 1140
atagtagcag cagaaggaga taagagaaga gaaagacacg agagattcag atcatgggaa 1200
gtaatgttga ggagctgtgg atttagcaat gttgctttaa gtccttttgc actttcacaa 1260
gctaaacttc tcttgagact tcattaccct tctgaaggat accagcttag tgtttcgagt 1320
acgagtaatt ctttcttctt gggttggcaa aatcaacccc ttttttccat atcttcttgg 1380
cgttaaatta taagggaaat taaaacccta aaaacaagat tttatctatc tgcatggtga 1440
aggacaaaga ggtcttc 1457
<210> 24
<211> 1548
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 24
aggttcttct tccttaatat tgagtcacag attagtacca ctactatagc caagaaaatg 60
tgaaatcata tagtactaaa tattaatttc agatgccaaa accataaatt tcccctcctc 120
catcattgaa aaccccctct gtcctttccc ctagagagac ccctttttcc tctctctctc 180
ctttctcttt ttattagacg catatattct ctcttctttc tctttctagg gttttcacct 240
gaaatagttt tatttcggtg atatgttagg atcctttggt tcatcatctc aatctcatga 300
tgaagaaact gatgatcaac ggcggagatt cagttccact tcccctgcaa tccaaatccg 360
gcaactactc attagctgcg cggagttaat ctcgcggtcc gatttctcgg ccgcaaacag 420
actcctcacc attttatcaa ctaactcttc cccttttggt gattcaactg aaagattagt 480
ccatcagttc actcgcgcac tttctcttcg cctcaaccgt tatatctctt cagccactaa 540
tttcttgaca ccatctaatg ttgttgaaag ttcaaatgat tcagctctac ttcagtcatc 600
ctatctttcc ctaaaccaag tgactccttt cattagattt agtcagctaa ctgctaatca 660
agcgattttg gaagctatta acgataacca acaagcgatc cacatcgttg attttgatat 720
taatcacggt gttcaatggc caccgttaat gcaagcacta gctgatcgtt accctcctcc 780
aactcttcgg attaccggta ctggaaatga ccttgatacc cttcgtagaa ccggagatcg 840
tttagctaaa tttgctcact ctttaggcct tagatttcag tttcaccctc ttttgattac 900
caataataat gacaatgatc atgacccttc aataatttct tctattgttc ttctccctga 960
tgagacatta gctatcaact gtgtatttta tcttcacagg ctcttgaaag accgcgaaaa 1020
gttaaggatt tttttgcata ggattaaatc catgaaccct aaagttgtaa cgctggccga 1080
gagagaagca aatcataatc acccactttt tttgcaaaga tttgtggagg ctttggatta 1140
ttatgcagct gtgtttgatt cattggaagc aactttgcca ccgagcagta gagagaggat 1200
gacagtggaa caagtttggt tcgggagaga aataattgat atagtagcag cagaaggaga 1260
taagagaaga gaaagacacg agagattcag atcatgggaa gtaatgttga ggagctgtgg 1320
atttagcaat gttgctttaa gcccttttgc actctcacaa gctaaacttc tcttgagact 1380
tcattaccca tctgaaggat accagcttag tgtttcgagt acgagtaatt ctttcttctt 1440
gggttggcaa aatcaacccc ttttttccat atcttcttgg cgttaaattt aaaaccctaa 1500
aaaacaagat ttttatctat ctgcatggtg aaggacaaag aggtcttc 1548
<210> 25
<211> 1936
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 25
gtccatctgt ctatataggt agaatgagag taaaggagaa aacatatcct cctctccatt 60
tctgtagaca aagattctca aagagaaaca aattaaacac tagagagtga gagagtgcta 120
taagaaaaag aatatgggga gagctccatg ttgtgataaa gcaaatgtga agagagggcc 180
atggtctcct gaagaagatg ctaaactcaa agatttcatt cacaaatatg gaactggtgg 240
aaattggatt gctcttcctc aaaaagctgg taacaacaac ttctactcca ctagtcctct 300
atgtgtatgt attttattat tattattatt attattatta ttattattat tattattatt 360
attcatgaat cgaagggaca aaggtctaaa tctcagtggg tcgtggtagc aaggccattc 420
cgccatttat aatatcttct tgcaaattcc accagtttca tatgtgtatg tttttttctt 480
attagtcata aatcaaagcg acgaagggtt aaatttcagt tgattgtgat agcaaggtca 540
cactctaccg cttataatat ctcgtggcgt atttaacatt gtttgtatgt atatgtttga 600
gtataaaggg aggaaagctt atatttatat ttgagtggat tgagtttttt tccttgttgc 660
tgcattattt atgatttgat gagatttatg ttgggaactg caggactaaa gagatgtggg 720
aagagttgta gattgagatg gctaaattat ttaaggccta acattaaaca tggtgatttt 780
tctgaggaag aagatagagt tatttgcacc ttgtattcca ccattggaag caggtaatat 840
atatatacct ttttttggtc gtaatttttt tttcattttt tatcatcttt ctgatgaatt 900
tgagactgaa acaaaaactg ttcccactaa aaatggaaaa gtaaaacctc aataagtaag 960
aaaagggaaa aaacaatgag ggctcagaaa gaaatgcaaa tagtcagttg gatttttaat 1020
taaagattct gccatttatg gacatatttt tctgcatgca tgccaggttt agatctaaga 1080
tcaagtcttt atttactcac ttacagatgt ttaattatta agacaaagtt ccaatttttc 1140
ttctttcttc tctttctttt tgtggaaatt ttttctctag taaaccaatt aatttttgtt 1200
ataacatgtg caatataata tgttaacagg tggtcaataa tagcagctca attaccggga 1260
agaactgaca atgatatcaa gaattactgg aatactaagc tcaagaaaaa acctatggga 1320
ttaatgcaat caactaacca aagaaaatca ccatattttc cagctactaa ttctcttcaa 1380
acccaacccc agataaattc aagtcttttt agagacttat attacacccc aaataatagg 1440
cctaatatta caggcctaaa tcatcagtcc atttcttctg cccaccagac aaattttctc 1500
tacactaata ataacatgaa ctttcctaat ttgggtgcta caaataatca atatccttat 1560
aatatccaaa gtcataattt acttatgttt ggagaagcaa gttgttcttc atcagatgga 1620
agttgcagcc aaatgagttt tggtaaagaa atcaagagag aagaaattat gagtaatagt 1680
ttacaacaag gtcaaatttc aagtgttaat gcttttgaag aaaaccacca gaattttact 1740
cttgattatg gcaatagtag tagtaattgg gtggatcaaa aaccaaatgt gtattttggt 1800
actactacta ctcaagtact tcagtatgat aatgttgaag aagttaagca gcagctaaca 1860
agttgtacca atggcaacaa tggtagtact attggatgta acaacaacaa cagtatgttc 1920
gtgttcaatg atgaga 1936
<210> 26
<211> 1752
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 26
ccacttgtct atatagcaag aaagagagta aaggagaaaa catattctcc tctccatttc 60
tgtagacaag attctcaaaa agaaacaaat taaacactag agagtgagag agaactataa 120
gaaaaagaat atggggagag ctccatgttg tgataaagca aatgtgaaga gagggccatg 180
gtctcctgaa gaagatgcta aactcaaaga tttcattcac aaatatggaa ctggtggaaa 240
ttggattgct cttccccaaa aagcaggtaa caacaacttc tactccctta ttcccagaat 300
cgaagcgaca aagggttaaa tctcagtgga ttgtggtagc aagatcatat tctatcgctt 360
acaatatctc gtcgcgtatt taacactttc gtatgtatat gtttgaatat agggggaggg 420
aagcttacat taatatttat actttgagtg gattaagttt ttttttggtt gcttcattat 480
ttatgatttt gatgagatat atgtttggaa ctgcaggact aaagagatgt gggaagagtt 540
gtagattgag atggctaaat tatctaaggc ctaatatcaa acatggtgat ttttcggagg 600
aagaagatag agttatttgc agcttgtatt ccaccattgg aagcaggtac aatatacctt 660
tttttagtct taaattgttt tccatttttt atcatctttc tgatgaattt gagactgaaa 720
caaaaactgt tcccactaaa aatggaaaag aagaacctta ataaataaga aaagggaaaa 780
aacaatgagg gctcagaaag aaatgcaaat agtctgttgg atttttaatt aaagattctg 840
ccatttatgg acattttttt ctgcatgcat gccaggttta gatctaagat caagtcttta 900
tttactcact tacagctgtt taagtattac tactacaaaa ttccaacgtt tcttcttttc 960
tctctttttt tttttttttt tggaaaactt ttccttttgt aaaccaatta aattttgtta 1020
taacatatgc aatatattat gttaacaggt ggtcaataat agcagctcaa ttaccaggaa 1080
ggactgacaa tgatatcaag aattactgga atactaaact caagaaaaag cttatgggat 1140
taatgcaatc aacaaaccaa agaaaatcac catattttcc agctactaat tctcttcaag 1200
cccaacccca gataaattca agtcttttta gagacttata ttacaaccca aataataggc 1260
ctattattac aggcctaaat cagtccattt cttctgccca ccagccaaat tttctctaca 1320
ctaatagtaa catgaatttt cctaatttgg gtgctacaaa tagtcaatat ccttataata 1380
ttcaaagtca taatttactt atgtttggag aagcaagttg ttcttcatca gatggaagtt 1440
gtagccaaat gagttttggc aaagaaatca agagagagga aattatgagt aattgtttac 1500
aacaaggtca aatttcaagt gttaatgctt ttgaagaaaa tcagaatttc actcttgatt 1560
atggtaacag tagtagtaat tgggtggatc aaaaaccaaa tgtgtatttt ggaaatacta 1620
ctactactac tcaagtactt cagtatgatg ttgaagaagt taagcagcag ctaacaagtt 1680
gtaccaatgg caacaatggc agtactattg gatgtaacaa caacaacagt atgttcgtgt 1740
tcaatgatga ga 1752
<210> 27
<211> 648
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 27
gcatggacaa tctcatcttc tcaaacttca taaagatatc tttaaaaaaa agagaaaata 60
gaggtaatta gttgtatcaa tggatcaaca acattccact tgtttttctt cttcaagtaa 120
aattaatgac aaagaaaaga agaaaaaaag atcagttgtg aaactatcaa ctgatccaca 180
aagtgtagca gctcgtgaaa gaaggcatag aatcagtgat cgtttcaaga ttttgcagag 240
tttaatccct ggtggttcaa aaatggatac agttactatg ttagaagaag caattcacta 300
tgtcaaattt cttaagactc aaatatggct gcatcaaacc gtgattaata ttgtagatga 360
ttatgataat ccaaattatc atgatcagtt gctaatggct catgactcta attttgctaa 420
ttattatcct catgaaatgg tggaatattg cccagctcct gttgagaatg cacaaataaa 480
ttataacttg gaccagctgc agcttccagg ttatgcattt tcagatgggg atcaattcca 540
aggagaagaa actaatatta ctggtgattc ttttatgtac tattagttag ttaattatgt 600
tgcctaagtt taattagaat acgtagtgtg tggtagtatg gtatgttg 648
<210> 28
<211> 773
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 28
atataaacca tgcatggaca atctcctctt ctcaaacttc ataaagatat tatattaaaa 60
aaaataaaga agaagagaag atagaggtaa ttagctatag caatggatca acaacattcc 120
acttgttttt cttcttcaag caaaattaat gacaaagaaa agaagaaaaa aggatcagtt 180
gtgaaactat caactgatcc acaaagtgta gcagctcgtg aaagaaggca tagaatcagt 240
gatcgtttca agattttgca gagtttagtc cctggtggtt ctaaaatgga cacagttaca 300
atgttagaag aagcaattca ctatgtcaaa tttctcaaga tgcaaatatg gctgcatcaa 360
accatgatta atattgtaga tgattatgat aatccaaatt atcatcatca gttgctaatg 420
gctcatgact ctaattttgc taattattat cctcatgaga ataactcaac tcctgttgag 480
aatgcacaaa taaattataa cttggaccag ctgcagcttc caggttatgc attttcagat 540
ggagatcaat tccaaggaga agaaactaat atttctggtg atgcttttat gtactattaa 600
ttagtaatta gttaattatg ttgcctaagt ttaattagaa tacgtagtgt gtggtagtat 660
ggtatgttgt tttctctctt tctatctagc agcctaatga tgggtttgtg ttaattaatt 720
agatgtagta aattgtaagt gttggttagt tgattaagta tgttgcaagt ttg 773
<210> 29
<211> 3335
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 29
tactatcact taataccatc attcatcatg ttgtcaatgg aagaaatatt gtgtgaacta 60
agtagagaag acatgaataa tgagaaaggt ctaccacctg gttttaggtt tcatcctact 120
gatgaagagc ttatcacttt ctaccttgcc tctaaggttt ttaacggcac cttttgtggt 180
attcagattg ctgaagttga tctcaacaga tgtgagccct gggaacttcc aggtaacaca 240
cacacacaca cacattccct tcattaaatt cttcttttgt tcatgtccca aaaagtacaa 300
ggaaaaaata gtagtaactc aaaaggaagc tgatgaaaag tctttttctt atttttttat 360
ttttaactcc ttgtcttgaa catggtttta aagttttgga atttgtctgt tgtaaacaca 420
tgtgatggaa tgtagtcgta gtatatatgg cagacgtact actgttttaa gatattcttc 480
attgctttgt cacgagaaat atttactact agtaatattt aaaattatat aatgagaata 540
tgagaagatt ctagtctaaa atacattaat aattggagat tcttgtctaa aatactttag 600
tcttcttctt ttgcatgtgc atatgatatc tcacaaagaa agttttttgt gaaattccac 660
tattaatacg tacctcacgt catttatgat ttaccagcta cgttaagttc ttaataatac 720
atgtcctact ttggacctat aaatttcaga catctctctc ttttgttacc tgtcatttat 780
gatttaccag cttcggtaag ttcttaacag tagtaagaca tgtacttgaa atatgttgtc 840
cactgattct taaccctttc ctagccatag aatatagtag taggcaggag cggatttagg 900
ggcgcaaggg tgttcaccca aattatacta tatatatata aggcaaaatc tgttttttat 960
ctctatatat taagttttga atgctctcaa cacaatccaa aagtatagtt tagtggtcaa 1020
agggattcaa aatctacata aggtcatggg ttcaatttca actagctaca aaaaaaaaat 1080
ttgaacccct tcgtagagat cccgcctatg catctcctgt tgttgcagaa agcaattaat 1140
atgtgtcaaa tctacttcta tgattattca ttgaaactaa aaaatacagt agaaaatatg 1200
tgtaccagct agttattatt gcgcacatta tatgaatgcg actattatag taatttaatg 1260
gtatgtttta gtaattttga aagtgaaaat tgcagaagtg gcaaagatgg gggaaagaga 1320
atggtatttc tttagcttaa gggacagaaa atacccaacc ggactaagaa caaaccgggc 1380
aacaggagct ggttattgga aagctacagg aaaagataga gaagtgtaca gtgcaacaaa 1440
tggagcactc cttgggatga agaaaacatt ggttttttac aaaggaagag caccaaaggg 1500
tgagaaaacc aaatgggtta tgcatgaata tcgtcttgac ggcgattttt cctaccgtta 1560
ctcttctaag gtaaatttct aatcctatct atcgatttga aaatagcacg ctcatctcgc 1620
ttttcgttca taataaataa ccaatttatt tttaattttt gttcaaaata tgtgacatat 1680
tatcttattt cagtttagac gatgacatat ttcgattatc gtgagagtaa tagttgatta 1740
cttgtagggc tatattgtac gtatctatag ggtttgtcct ttttagggtt gacttgttta 1800
gtaagtctcg tgatattaat tgatgtgatg ttgacttgat tgatggatag atggatatga 1860
gaaggtgttg ttatagtacc ctaaagataa ttgccgactc ttttcacttt gctgattctt 1920
aagaaaacat agtgaaaatt aaaaattatc ttggaatttt atgtacaaat tttaatggtt 1980
atttaatttc atgttcaagg gtattcccta accaatgtca cttaaaatat gtactgtaga 2040
gttattaggt gctttgacta aaaagattag tatactatca cttgacatat gtggttatta 2100
gtgttatcac tactaaaaac gggaattaac cactgacacg ttctgtagct aaacataaat 2160
tagcaatgat tataaataaa ttatgttagc tacgagtgaa ttaaagagta gtataacccg 2220
tcacttaggt gctttgatta aaaagagtag tataatataa tgtcgcttga aatatgtggt 2280
tattaagtcc tttcactatt aaaatcagaa attagcgaca tgcaaatttt gtaactaaac 2340
ggaaattagc gacaaattat aaagaaatta tgttttctat gagtgattta gcgacagatt 2400
aacgatgaag ttcctagtta attctagttc tttttgtaga gtaatttgtt aaaaagttgt 2460
aataaaattg gcaggaggaa tgggtgatat gcagaatact acacaaaata ggggagaaga 2520
aaaatccaat ataccaagct gcaggacaaa actatggcta ccctacaagc ttgaaaacat 2580
ggccatcatc atcttttctt aacacagcaa catcagcaga agcagctcca aatcctatat 2640
tggctgaaac accaaatcca aaaaccacaa caactacaca ttggcaagaa tcattccaaa 2700
tatcacaaaa ctcaatgcaa tcactgcaca acttttatct atttcaccac caagaaaacg 2760
accttatgaa atccctcttc aaccccatta atgtttccca aacaaacctc ttcccaataa 2820
ataatagtgt cctttcttct gctacctcct tttctacatc ccaaagcaca aaaaaataca 2880
aagaagacat aaacaaaaac tcgtcactat catctttcct cgttagcaat tcaaagaaaa 2940
atgaaaaaca tcaagtccca ctcatgcagg ctaacacaac aatgaaaaca gaagccagtt 3000
tttcaccata ttctggttgt tacaatgatc aaaaccctat ggctacgaat tttggtatga 3060
ataattcaac agattggagt ttagtaggca tagaagggat gcattttaat ggtggatgta 3120
ctcagtctca gatggtgttg gatcacatgc attgtcccat caaaatagct gcagaatctt 3180
ggcctcttga tctctaaaaa tagaagattt tgtttttaat aaattctact gtaggatgat 3240
atggtaatta attattactc ctgttatatc atccttatct atgaatagct atcctctaag 3300
tatataaaag taattcaggc tgctctttat attct 3335
<210> 30
<211> 3339
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 30
tacttccctt tctccctttg gtttcagtgc ctaatttgtt ttcttttctt ttctcttttg 60
tatatatata taatcctcca ctttctaacc agctactaca tcacttaata tcattcatta 120
tgttgtcaat ggaagaaata ttgtgtgaac ttagtagaga tgacatgaat aatgagaaag 180
gtctaccacc tggttttagg tttcatccta ctgatgaaga gcttatcact ttctaccttg 240
cctctaaggt ttttaacggc accttttgtg gtattcagat tgctgaagtt gacctcaaca 300
gatgtgagcc ctgggaactt ccaggtaaca cacacacaca cattgccttc attatattct 360
tcttttgttc atgtcccaaa gtacagggaa aaaatagtag taactcaaaa ggaagctgat 420
gagagtcttt ttttaccccc tttgtcttga aaatatatgg ttttaaagtt ttggaatttg 480
tctgttgtaa atacatgtaa tggaatgtag tagtatggca gacgtactag ctgttttaag 540
atattcttca ttgctttatc atcagaaata tttattcata tctaaaacaa ataatgagag 600
tatgagaaga ttcttgtcta aaatacatta aatgggatat tcttgtctaa aatactttag 660
tcattcttct tttgcatgtg catatgatat ctcaccaaga aagtttttct gtgaagttcc 720
actattgata cgtaccctca cgtcattaat tagttattta ccagcttcgc taagttctta 780
ataatacatg tccttgaatt ataatgccct ttcgacctat aaatttcaga catatctttc 840
ttttgttacc tcatgtcatt tatgatttac cagcttcgct aagttcttaa tagtagtaag 900
acatgtacat gaaatatgtt gtccactgac tcataaccct ttcctagcca tagaatatag 960
taggagtact attttcttct ttccttacat ttttaaaaat gaaaatgtat gcatctccct 1020
gttgttgcag aaagaaatta atatgggtca aatctacctc tatgattact tattaaaaca 1080
agcaaaatac agtagaaaat atgtgtacca gctagttgtt attattgcgc acatgcatta 1140
acatgaatgc gactattata gtaatttaat ggtatgttct agtaattttg aaagtgaaaa 1200
ttgcagaagt ggcaaagatg ggggagagag aatggtattt ctttagctta agggacagaa 1260
aatacccaac cgggctaaga acaaaccggg caacaggagc aggttattgg aaagctacag 1320
gaaaagatag ggaagtgtac agtgcaacca atggagcact ccttgggatg aagaaaacac 1380
tggtttttta caaaggaaga gcaccaaagg gtgagaaaac caagtgggtt atgcacgaat 1440
atcgtcttga cggtgatttt tcttaccgct actcttctaa ggtaaatttc tcatccttat 1500
tactcgctat ttagatgata tagttaatta ctcggagatt gagaaaatta aagggagtaa 1560
ttaattgtag agctttattg tatgtagggt ctataggttt tgtccttttt agggttgact 1620
tgtttagtaa gtctcgtgat attaattgac gtgatgtgga cttgattgat ggatatgagg 1680
tgttgttata gtactctaaa gatacttgcc aaatcttttc actttgctga ttcttaagaa 1740
aacatagcga aaatcatctt ggaatttcat gtactaaaat taaagattat ttcatgttca 1800
agggtattcc ctaaacctaa tgtcacatta aaaagttatt atagagttct taggtgcttt 1860
gattaaaaag agtactagtg taatgtcact cgacatgtct ttattaggtg ctttcactag 1920
tgtaatgtca cttgacatgt ctttattccc taaacctaat gtcacgttaa aaagatatta 1980
tagagttctt aggtgcttta aataaaaaga gtactagtgt aatgtcactt gacatgtctt 2040
tattaggtgc tttcactaat aaaaacaaaa attatatagc aatgattata aaaaaaaaat 2100
tgttagctac gagcgattac ccgtcactta ggtgctttga ttaaaagaga agtataatgt 2160
cgcttgaaat atgtggttat taggtgcttt tactattaaa aatatgaatt ggcgatggac 2220
aaattctgta gttaattata aagcagacac attataaaga aattatgtta gctacgagtg 2280
atttagtgac agatttagga caaagtttgt agctaattct aatttttttt gtaatgtttg 2340
ttaaaaaatt gtaataaact tggcaggagg aatgggtgat atgcagaata ctacacaaaa 2400
taggggagaa gaaaaatcca atataccaag ctgcaggaca aaactatggc taccctacaa 2460
gcttgaaaac atggccatca tcatcttttc tcaacacagc agctccaaat cccatattgg 2520
ctgaaacacc aaatccaaaa accacaacta ctacacattg gcaagaatca ttccaaatat 2580
cacaaaactc agtgcaatca ctgcacaacc tttatctatt tcaccaccaa gaaaacgacc 2640
ttatgaaatc cctcttcagt cccattaatg tttcccaaac aaacctcttc ccaataaata 2700
atagtgacct ttcttctgct gcctcctttt ctacatccca aagcaccaaa aaatacaaag 2760
aagacataaa caaaaactcg tcaatatcat ctttcctctt tagcaattcc ttttgcactt 2820
caaagaaaaa tgaaaaacag caagttccac taatgcaggc taacacaaca atgaaaacag 2880
aagctagttt ttcaccatat tctggttgtt acaatgatca aaaccctatg gcttcgactt 2940
ttgggatgaa taattcatca gattggagtt tagtaggcat agaagggatg cattttaatg 3000
gtggatgtac tcagtctcag atggtgttgg atcacatgaa ttgtcccatc aaaatcactg 3060
cagaatcttg gcctctcgat ctctaaaaat agaagagttg tttttccata atttctatag 3120
taggatgata tggtaattaa ttatgactac tgttatgtca tcctctatat atagctatcc 3180
gctctagtat atgtaatctt tgtaattaat ttaggctgct ttattctgaa agatgttgct 3240
ttctccttaa ggatatatct agctagtacc gctatgtaag atatatcttt cttttctcga 3300
ctaatgtaaa gttgcaatct attgatggga gtatttata 3339
<210> 31
<211> 445
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 31
gcctatgtag cttcgtcaat gaaatcttgt tcatatgcat atcctgggat gaggcctacc 60
agatttaccg gaagtcagat aataatgcca cttggccata caattgaaca tgaagagatg 120
cttgaggtta ttagattgga aggacactct attggccagg aagatacttt tatgccaaga 180
gatgttcacc ttctccagat gtgtagtgga actgatgaga atgctgtcgg agcttgttct 240
gaactagttt ttgctgcaat tgatgagatg tttccagatg atgcacccct gttgccctcc 300
gggtttcgta tcattcctct cgagtcaaaa tcaagcgatc cccaggatac atcgaatgct 360
catagaacac tggatctggc atcaagtctt gaagttggcc cagcaacaaa ccctgctact 420
ggagatgtgg tctctggcta cagtg 445
<210> 32
<211> 565
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 32
ctcaagaaaa agcttatggg attaatgcaa tcaacaaacc aaagaaaatc accatatttt 60
ccagctacta attctcttca agcccaaccc cagataaatt caagtctttt tagagactta 120
tattacaacc caaataatag gcctattatt acaggcctaa atcagtccat ttcttctgcc 180
caccagccaa attttctcta cactaatagt aacatgaatt ttcctaattt gggtgctaca 240
aatagtcaat atccttataa tattcaaagt cataatttac ttatgtttgg agaagcaagt 300
tgttcttcat cagatggaag ttgtagccaa atgagttttg gcaaagaaat caagagagag 360
gaaattatga gtaattgttt acaacaaggt caaatttcaa gtgttaatgc ttttgaagaa 420
aatcagaatt tcactcttga ttatggtaac agtagtagta attgggtgga tcaaaaacca 480
aatgtgtatt ttggaaatac tactactact actcaagtac ttcagtatga tgttgaagaa 540
gttaagcagc agctaacaag ttgta 565
<210> 33
<211> 364
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 33
gaagaaactg atgatcaacg gcggagattc agttccactt cccctgcaat ccaaatccgg 60
caactactca ttagctgcgc ggagttaatc tcgcggtccg atttctcggc cgcaaacaga 120
ctcctcacca ttttatcaac taactcttcc ccttttggtg attcaactga aagattagtc 180
catcagttca ctcgcgcact ttctcttcgc ctcaaccgtt atatctcttc agccactaat 240
ttcttgacac catctaatgt tgttgaaagt tcaaatgatt cagctctact tcagtcatcc 300
tatctttccc taaaccaagt gactcctttc attagattta gtcagctgac tgctaatcaa 360
gcga 364
<210> 34
<211> 304
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 34
ttcttcaagc aaaattaatg acaaagaaaa gaagaaaaaa ggatcagttg tgaaactatc 60
aactgatcca caaagtgtag cagctcgtga aagaaggcat agaatcagtg atcgtttcaa 120
gattttgcag agtttagtcc ctggtggttc taaaatggac acagttacaa tgttagaaga 180
agcaattcac tatgtcaaat ttctcaagat gcaaatatgg ctgcatcaaa ccatgattaa 240
tattgtagat gattatgata atccaaatta tcatcatcag ttgctaatgg ctcatgactc 300
taat 304
<210> 35
<211> 437
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 35
acctggtttt aggtttcatc ctactgatga agagcttatc actttctacc ttgcctctaa 60
ggtttttaac ggcacctttt gtggtattca gattgctgaa gttgatctca acagatgtga 120
gccctgggaa cttccagaag tggcaaagat gggggaaaga gaatggtatt tctttagctt 180
aagggacaga aaatacccaa ccggactaag aacaaaccgg gcaacaggag ctggttattg 240
gaaagctaca ggaaaagata gagaagtgta cagtgcaaca aatggagcac tccttgggat 300
gaagaaaaca ttggtttttt acaaaggaag agcaccaaag ggtgagaaaa ccaaatgggt 360
tatgcatgaa tatcgtcttg acggcgattt ttcctaccgt tactcttcta aggaggaatg 420
ggtgatatgc agaatac 437
<210> 36
<211> 111
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 36
Met Ala Met Val Val Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu
100 105 110
<210> 37
<211> 116
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 37
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser
115
<210> 38
<211> 68
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 38
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys
65
<210> 39
<211> 398
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 39
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Met
245 250 255
Leu Arg Ile Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val
260 265 270
Thr Leu Ala Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln
275 280 285
Arg Phe Val Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu
290 295 300
Glu Ala Thr Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln
305 310 315 320
Val Trp Phe Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp
325 330 335
Lys Arg Arg Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu
340 345 350
Arg Ser Cys Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser
355 360 365
Gln Ala Lys Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln
370 375 380
Leu Ser Val Ser Ser Thr Ser Asn Ser Phe Phe Leu Gly Trp
385 390 395
<210> 40
<211> 145
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 40
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn
145
<210> 41
<211> 163
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 41
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met
<210> 42
<211> 838
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 42
Met Ala Met Val Val Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Ala Cys Gly Leu Val Ser Leu Glu Pro Thr Lys Ile Ala
210 215 220
Glu Ile Leu Lys Asp Arg Pro Ser Trp Phe Arg Asp Cys Arg Asn Val
225 230 235 240
Glu Val Phe Thr Met Phe Ser Ala Gly Asn Gly Thr Ile Glu Leu Leu
245 250 255
Tyr Thr Gln Ile Tyr Ala Pro Thr Thr Leu Ala Pro Ala Arg Asp Phe
260 265 270
Trp Thr Leu Arg Tyr Thr Thr Thr Leu Glu Asn Gly Ser Phe Val Val
275 280 285
Cys Glu Arg Ser Leu Ser Gly Thr Gly Ala Gly Pro Asn Ala Ala Ser
290 295 300
Ala Ser Gln Phe Val Arg Ala Gln Met Leu Pro Ser Gly Tyr Leu Ile
305 310 315 320
Arg Pro Cys Asp Gly Gly Gly Ser Ile Ile His Ile Val Asp His Leu
325 330 335
Asn Leu Glu Ala Trp Ser Ala Pro Glu Ile Leu Arg Pro Leu Tyr Glu
340 345 350
Ser Ser Lys Val Val Ala Gln Lys Met Thr Ile Ala Ala Leu Arg Tyr
355 360 365
Ala Arg Gln Ile Ala Gln Glu Thr Ser Gly Glu Val Val Tyr Gly Leu
370 375 380
Gly Arg Gln Pro Ala Val Leu Arg Thr Phe Ser Gln Arg Leu Ser Arg
385 390 395 400
Gly Phe Asn Asp Ala Ile Asn Gly Phe Ser Asp Asp Gly Trp Ser Leu
405 410 415
Leu Ser Ser Asp Gly Gly Glu Asp Val Ile Val Ala Val Asn Ser Arg
420 425 430
Lys Asn Ile Ala Thr Thr Ser Val Pro Leu Ser Pro Leu Gly Gly Ile
435 440 445
Leu Cys Ala Lys Ala Ser Met Leu Leu Gln Asn Val Pro Pro Val Val
450 455 460
Leu Val Arg Phe Leu Arg Glu His Arg Ser Glu Trp Ala Asp Phe Asn
465 470 475 480
Val Asp Ala Tyr Val Ala Ser Ser Met Lys Ser Cys Ser Tyr Ala Tyr
485 490 495
Pro Gly Met Arg Pro Thr Arg Phe Thr Gly Ser Gln Ile Ile Met Pro
500 505 510
Leu Gly His Thr Ile Glu His Glu Glu Met Leu Glu Val Ile Arg Leu
515 520 525
Glu Gly His Ser Ile Gly Gln Glu Asp Thr Phe Met Pro Arg Asp Val
530 535 540
His Leu Leu Gln Met Cys Ser Gly Thr Asp Glu Asn Ala Val Gly Ala
545 550 555 560
Cys Ser Glu Leu Val Phe Ala Ala Ile Asp Glu Met Phe Pro Asp Asp
565 570 575
Ala Pro Leu Leu Pro Ser Gly Phe Arg Ile Ile Pro Leu Glu Ser Lys
580 585 590
Ser Ser Asp Pro Gln Asp Thr Ser Asn Ala His Arg Thr Leu Asp Leu
595 600 605
Ala Ser Ser Leu Glu Val Gly Pro Ala Thr Asn Pro Ala Thr Gly Asp
610 615 620
Val Val Ser Gly Tyr Ser Ala Arg Ser Val Leu Thr Ile Ala Phe Gln
625 630 635 640
Phe Pro Phe Glu Asp Asn Leu Gln Asp Asn Val Ala Thr Met Ala Arg
645 650 655
Gln Tyr Val Arg Ser Val Val Ser Ser Val Gln Arg Val Ala Met Ala
660 665 670
Ile Ser Pro Ala Gly Val Asn Ser Thr Phe Gly Ser Lys Leu Ser Pro
675 680 685
Gly Ser Pro Glu Ala Val Thr Leu Ser His Trp Ile Cys Gln Ser Tyr
690 695 700
Ser Tyr His Met Gly Thr Glu Leu Leu Gln Thr Asp Ser Arg Gly Asp
705 710 715 720
Glu Ser Val Leu Lys Asn Leu Trp Gln His Gln Asp Ala Ile Leu Cys
725 730 735
Cys Ser Leu Lys Ser Leu Pro Val Phe Ile Phe Ala Asn Lys Ala Gly
740 745 750
Leu Asp Met Leu Glu Thr Thr Leu Val Ala Leu Gln Asp Ile Thr Leu
755 760 765
Asp Lys Ile Phe Asp Glu Ser Gly Arg Lys Val Leu Phe Ala Glu Phe
770 775 780
Pro Lys Ile Met Glu Gln Gly Phe Ala Tyr Leu Pro Gly Gly Ile Cys
785 790 795 800
Met Ser Ala Met Gly Arg His Ile Ser Tyr Glu Gln Ala Ile Ala Trp
805 810 815
Lys Val Phe Ala Ser Glu Glu Thr Val His Cys Leu Ala Phe Ser Phe
820 825 830
Ile Asn Trp Ser Phe Val
835
<210> 43
<211> 839
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 43
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Ala Cys Gly Leu Val Ser Leu Glu Pro Thr Lys Ile Ala
210 215 220
Glu Ile Leu Lys Asp Arg Ser Ser Trp Phe Arg Asp Cys Arg Asn Val
225 230 235 240
Glu Val Phe Thr Met Phe Ser Ala Gly Asn Gly Thr Ile Glu Leu Leu
245 250 255
Tyr Thr Gln Ile Tyr Ala Pro Thr Thr Leu Ala Pro Ala Arg Asp Phe
260 265 270
Trp Thr Leu Arg Tyr Thr Thr Thr Leu Glu Asn Gly Ser Phe Val Val
275 280 285
Cys Glu Arg Ser Leu Ser Gly Thr Gly Ala Gly Pro Asn Ala Ala Ser
290 295 300
Ala Ser Gln Phe Val Arg Ala Gln Met Leu Pro Ser Gly Tyr Leu Ile
305 310 315 320
Arg Pro Cys Asp Gly Gly Gly Ser Ile Ile His Ile Val Asp His Leu
325 330 335
Asn Leu Glu Ala Trp Ser Ala Pro Glu Ile Leu Arg Pro Leu Tyr Glu
340 345 350
Ser Ser Lys Val Val Ala Gln Lys Met Thr Ile Ala Ala Leu Arg Tyr
355 360 365
Ala Arg Gln Ile Ala Gln Glu Thr Ser Gly Glu Val Val Tyr Gly Leu
370 375 380
Gly Arg Gln Pro Ala Val Leu Arg Thr Phe Ser Gln Arg Leu Ser Arg
385 390 395 400
Gly Phe Asn Asp Ala Ile Asn Gly Phe Ser Asp Asp Gly Trp Ser Leu
405 410 415
Leu Ser Ser Asp Gly Gly Glu Asp Val Ile Val Ala Val Asn Ser Arg
420 425 430
Lys Asn Ile Ala Thr Thr Ser Val Pro Leu Ser Pro Leu Gly Gly Ile
435 440 445
Leu Cys Ala Lys Ala Ser Met Leu Leu Gln Asn Val Pro Pro Ala Val
450 455 460
Leu Val Arg Phe Leu Arg Glu His Arg Ser Glu Trp Ala Asp Phe Asn
465 470 475 480
Val Asp Ala Tyr Val Ala Ser Ser Met Lys Ser Cys Ser Tyr Ala Tyr
485 490 495
Pro Gly Val Arg Pro Thr Arg Phe Thr Gly Ser Gln Ile Ile Met Pro
500 505 510
Leu Gly His Thr Ile Glu His Glu Glu Met Leu Glu Val Ile Arg Leu
515 520 525
Glu Gly His Ser Ile Gly Gln Glu Asp Ala Phe Met Pro Arg Asp Ile
530 535 540
His Leu Leu Gln Met Cys Ser Gly Thr Asp Glu Asn Ala Val Gly Ala
545 550 555 560
Cys Ser Glu Leu Val Phe Ala Ala Ile Asp Glu Met Phe Pro Asp Asp
565 570 575
Ala Pro Leu Leu Pro Ser Gly Phe Arg Ile Ile Pro Leu Glu Ser Lys
580 585 590
Ser Ser Asp Pro Gln Asp Thr Ser Asn Ala His Arg Thr Leu Asp Leu
595 600 605
Ala Ser Ser Leu Glu Val Gly Pro Ala Thr Asn Pro Ala Thr Gly Asp
610 615 620
Val Val Ser Gly Tyr Ser Ala Arg Ser Val Leu Thr Ile Ala Phe Gln
625 630 635 640
Phe Pro Phe Glu Asp Asn Leu Gln Asp Asn Val Ala Thr Met Ala Arg
645 650 655
Gln Tyr Val Arg Ser Val Val Ser Ser Val Gln Arg Val Ala Met Ala
660 665 670
Ile Ser Pro Ala Gly Val Asn Ser Thr Phe Gly Ser Lys Leu Ser Pro
675 680 685
Gly Ser Pro Glu Ala Val Thr Leu Ser His Trp Ile Cys Gln Ser Tyr
690 695 700
Ser Tyr His Met Gly Thr Glu Leu Leu Gln Ala Asp Ser Arg Gly Asp
705 710 715 720
Glu Ser Val Leu Lys Asn Leu Trp Gln His Gln Asp Ala Ile Leu Cys
725 730 735
Cys Ser Leu Lys Ser Leu Pro Val Phe Ile Phe Ala Asn Lys Ala Gly
740 745 750
Leu Asp Met Leu Glu Thr Thr Leu Val Ala Leu Gln Asp Ile Thr Leu
755 760 765
Asp Arg Ile Phe Asp Glu Ser Gly Arg Lys Val Leu Phe Ala Glu Phe
770 775 780
Pro Lys Ile Met Asp Gln Gly Phe Ala Tyr Leu Pro Gly Gly Ile Cys
785 790 795 800
Met Ser Ala Met Gly Arg His Ile Ser Tyr Glu Gln Ala Ile Ala Trp
805 810 815
Lys Val Phe Ala Ser Glu Glu Thr Ser Val His Cys Leu Ala Phe Ser
820 825 830
Phe Ile Asn Trp Ser Phe Val
835
<210> 44
<211> 407
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 44
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Lys Leu Arg Ile
245 250 255
Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val Thr Leu Ala
260 265 270
Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln Arg Phe Val
275 280 285
Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu Glu Ala Thr
290 295 300
Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln Val Trp Phe
305 310 315 320
Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp Lys Arg Arg
325 330 335
Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu Arg Ser Cys
340 345 350
Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser Gln Ala Lys
355 360 365
Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln Leu Ser Val
370 375 380
Ser Ser Thr Ser Asn Ser Phe Phe Leu Gly Trp Gln Asn Gln Pro Leu
385 390 395 400
Phe Ser Ile Ser Ser Trp Arg
405
<210> 45
<211> 410
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 45
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Met
245 250 255
Leu Arg Ile Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val
260 265 270
Thr Leu Ala Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln
275 280 285
Arg Phe Val Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu
290 295 300
Glu Ala Thr Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln
305 310 315 320
Val Trp Phe Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp
325 330 335
Lys Arg Arg Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu
340 345 350
Arg Ser Cys Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser
355 360 365
Gln Ala Lys Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln
370 375 380
Leu Ser Val Ser Ser Thr Ser Asn Ser Phe Phe Leu Gly Trp Gln Asn
385 390 395 400
Gln Pro Leu Phe Ser Ile Ser Ser Trp Arg
405 410
<210> 46
<211> 167
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 46
Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn Asp
1 5 10 15
Lys Glu Lys Lys Lys Lys Arg Ser Val Val Lys Leu Ser Thr Asp Pro
20 25 30
Gln Ser Val Ala Ala Arg Glu Arg Arg His Arg Ile Ser Asp Arg Phe
35 40 45
Lys Ile Leu Gln Ser Leu Ile Pro Gly Gly Ser Lys Met Asp Thr Val
50 55 60
Thr Met Leu Glu Glu Ala Ile His Tyr Val Lys Phe Leu Lys Thr Gln
65 70 75 80
Ile Trp Leu His Gln Thr Val Ile Asn Ile Val Asp Asp Tyr Asp Asn
85 90 95
Pro Asn Tyr His Asp Gln Leu Leu Met Ala His Asp Ser Asn Phe Ala
100 105 110
Asn Tyr Tyr Pro His Glu Met Val Glu Tyr Cys Pro Ala Pro Val Glu
115 120 125
Asn Ala Gln Ile Asn Tyr Asn Leu Asp Gln Leu Gln Leu Pro Gly Tyr
130 135 140
Ala Phe Ser Asp Gly Asp Gln Phe Gln Gly Glu Glu Thr Asn Ile Thr
145 150 155 160
Gly Asp Ser Phe Met Tyr Tyr
165
<210> 47
<211> 165
<212> PRT
<213> 红花烟草(Nicotiana tabacum)
<400> 47
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Gly Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Arg Arg His Arg Ile Ser Asp Arg
35 40 45
Phe Lys Ile Leu Gln Ser Leu Val Pro Gly Gly Ser Lys Met Asp Thr
50 55 60
Val Thr Met Leu Glu Glu Ala Ile His Tyr Val Lys Phe Leu Lys Met
65 70 75 80
Gln Ile Trp Leu His Gln Thr Met Ile Asn Ile Val Asp Asp Tyr Asp
85 90 95
Asn Pro Asn Tyr His His Gln Leu Leu Met Ala His Asp Ser Asn Phe
100 105 110
Ala Asn Tyr Tyr Pro His Glu Asn Asn Ser Thr Pro Val Glu Asn Ala
115 120 125
Gln Ile Asn Tyr Asn Leu Asp Gln Leu Gln Leu Pro Gly Tyr Ala Phe
130 135 140
Ser Asp Gly Asp Gln Phe Gln Gly Glu Glu Thr Asn Ile Ser Gly Asp
145 150 155 160
Ala Phe Met Tyr Tyr
165
<210> 48
<211> 2640
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 48
ttgtttggga ttttggggtt tgagggcaaa tggctatggt ggtacagcag catagggaga 60
gtagtagtgg tagtattaca aaacatcttg acagtagtgg aaagtatgtc cggtatacag 120
ctgagcaagt ggaggcatta gagagggttt atgcagagtg ccctaaacct agctcgttgc 180
gccgccagca attgatccgc gaatgcccta ttctgtcgaa tatcgagcct aagcagatca 240
aagtttggtt tcaaaacaga aggtgtcgag agaagcaaag gaaagagtct tctagactac 300
agactgtaaa tagaaagctg tctgcaatga ataaactatt aatggaggag aatgatcgct 360
tgcaaaaaca ggtttcacag cttgtgtgtg aaaatggctt tatgcggcaa caattgcata 420
ctgcatcagc ggccactact gatgtaagtt gtgagtcagt ggttaccacc cctcagcatt 480
ccctcagaga tgctaacaac cctgctggac tgctgtcgat tgcagaggaa accttagcag 540
agttcctttc caaggctaca ggaactgctg ttgattgggt cccgatgcct gggatgaagc 600
ctggtccgga ttcagttggg atttttgcca tctcacacag ttgtagtgga gtggcagccc 660
gagcatgtgg tcttgttagt ttagagccga caaagattgc tgagatcctc aaagatcgac 720
catcttggtt ccgagactgc cggaacgttg aagttttcac gatgttttct gcaggaaatg 780
gaacaattga gcttttgtac acgcagatat atgctcctac caccttggct cctgcacgtg 840
atttttggac tctgagatac acaaccaccc tggagaatgg tagttttgtg gtttgtgaaa 900
gatccctctc tggtactgga gctgggccga atgctgcttc tgcttcccag tttgtaagag 960
ctcaaatgct tccgtccgga tatctaatcc gaccgtgtga cggtggagga tccattatac 1020
atattgttga ccatctgaat cttgaggcat ggagtgcccc tgagattttg cgtccacttt 1080
atgaatcgtc aaaagttgtg gcacagaaaa tgactattgc ggcactgcga tatgcaaggc 1140
aaatagctca ggagactagt ggggaggttg tatatggtct gggaaggcaa cctgcagttc 1200
ttcgaacatt tagccagaga ttaagcagag gcttcaatga cgccatcaat ggattcagtg 1260
atgatggctg gtcattgtta agttctgatg gtggtgaaga tgttatagtt gctgtcaatt 1320
caaggaagaa cattgccacc acttccgttc ctctttcacc gctgggaggc atcctttgtg 1380
ccaaagcatc aatgctactc cagaatgttc ctcctgtggt actggttcga tttctcaggg 1440
agcaccgttc agagtgggcg gactttaatg ttgatgccta tgtagcttcg tcaatgaaat 1500
cttgttcata tgcatatcct gggatgaggc ctaccagatt taccggaagt cagataataa 1560
tgccacttgg ccatacaatt gaacatgaag agatgcttga ggttattaga ttggaaggac 1620
actctattgg ccaggaagat acttttatgc caagagatgt tcaccttctc cagatgtgta 1680
gtggaactga tgagaatgct gtcggagctt gttctgaact agtttttgct gcaattgatg 1740
agatgtttcc agatgatgca cccctgttgc cctccgggtt tcgtatcatt cctctcgagt 1800
caaaatcaag cgatccccag gatacatcga atgctcatag aacactggat ctggcatcaa 1860
gtcttgaagt tggcccagca acaaaccctg ctactggaga tgtggtctct ggctacagtg 1920
cacgatctgt gttgacaatt gcttttcaat ttccattcga ggacaatctt caggacaatg 1980
tagctaccat ggcgcgccag tatgttcgca gtgtggtttc atctgtccaa cgggttgcca 2040
tggcaatatc tcccgcagga gtgaattcaa cattcgggtc caagctttct ccaggctccc 2100
ctgaagctgt aacgttgtcg cactggatct gccagagcta cagttatcac atggggacag 2160
agttgcttca aactgattcg aggggcgatg aatcagtgct aaaaaatctt tggcaacatc 2220
aggatgctat tttgtgctgc tcattgaagt ccctgccggt tttcattttt gctaataagg 2280
ctgggcttga tatgctggag acaaccttag ttgctttaca ggacattact ctagataaga 2340
tatttgatga atctggccgg aaagtgttgt tcgctgaatt tcccaagatc atggaacagg 2400
gttttgcgta cttgccgggt ggtatttgca tgtcagcaat gggacgacat atttcatatg 2460
aacaagctat tgcatggaaa gtctttgctt ctgaagaaac tgtccactgc ttagccttct 2520
catttattaa ctggtcattt gtttaatgtt gctgtcaaat ctcctttctt ttttttcctt 2580
tttgtttttt gacatcttcc tcacagagga cactgacagc caggaacaca gttgaacgga 2640
<210> 49
<211> 2726
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 49
aagctgtttg cagggaatat atctacttgg gggtgttttt atttcttaaa agggtgtttg 60
aaaatttgga aatcttgatt ttttttttgg tttgggattt tgaggtttga gggcaatggc 120
tatggttgca cagcagcaca gggagagtag tagtggtagt attacaaaac atcttgacag 180
tagtggaaag tatgtccggt atacagctga gcaagttgag gcattggaga gggtttatgc 240
tgagtgccct aagcctagct ccttgcgccg ccaacaattg atccgtgaat gccctattct 300
gtcgaatatc gagcctaagc agatcaaagt ttggtttcaa aacagaaggt gtcgagagaa 360
gcaaaggaaa gagtcttctc gactacagac tgtaaataga aagctgtctg caatgaataa 420
actattgatg gaggagaatg atcgcttgca aaaacaggtt tcgcagcttg tgtgtgaaaa 480
tggctttatg cggcaacagt tgcatactgc atcagcggcc actactgatg taagttgtga 540
gtctgtggta actacccctc agcattccct cagagatgct aacaaccctg ctggactgct 600
gtcgattgca gaggaaacct tagcagagtt cctttccaag gctacaggaa ctgctgttga 660
ttgggtcccg atgcctggga tgaagcctgg tccggattca gttgggattt ttgccatctc 720
acacagttgt agtggagtgg cagcccgagc atgtggtctt gttagtttag agccgacaaa 780
gattgctgag atcctcaaag atcgatcttc ttggttccga gattgccgga acgttgaagt 840
tttcacaatg ttttctgcag gaaatggaac aattgaactt ttgtacacgc agatatatgc 900
tcctaccacc ttggctcctg cacgtgattt ttggactctg agatacacaa ccaccctgga 960
gaatggtagc tttgtggttt gtgaaagatc cctctctggt actggagctg ggccgaatgc 1020
tgcttctgct tcccagtttg taagagctca aatgcttccg tctggatatc taatccgacc 1080
gtgtgacggt ggaggatcca ttatacatat tgttgaccac ctgaatcttg aggcatggag 1140
tgcccctgag attttgcgtc cactttatga atcgtcaaaa gttgtggcac agaaaatgac 1200
tattgcggca ctgcgatatg caaggcaaat agctcaggag actagtgggg aggttgtata 1260
tggtctggga aggcaacctg cagttcttcg aacatttagc cagagattaa gcagaggctt 1320
caatgatgcc atcaatggat tcagtgatga tggctggtca ttgttaagtt ctgatggtgg 1380
tgaagatgtt atagttgctg tcaattcaag gaagaacatt gccaccactt ccgttcctct 1440
ttcaccactt ggaggcatcc tttgtgccaa agcatcaatg ctactccaga atgttcctcc 1500
tgcggtactg gttcgatttc tcagggagca ccgttcagag tgggcggact ttaatgttga 1560
tgcctatgta gcttcctcaa tgaaatcttg ttcatatgca tatcctgggg tgaggcctac 1620
cagatttacc ggaagccaga taataatgcc actgggccac acaatagaac atgaagagat 1680
gcttgaagtt attagattgg aagggcactc tattggccag gaagatgctt ttatgccgag 1740
agatattcac cttctccaga tgtgtagtgg aaccgatgag aatgctgtcg gagcttgttc 1800
tgaactagtt tttgctgcaa ttgatgagat gtttccagat gatgcacccc tgttgccctc 1860
cgggtttcgt atcattcctc tcgagtcaaa atcaagcgat ccccaggata catcgaatgc 1920
tcatagaaca ctggatctgg catcaagtct tgaagttggc ccagcaacaa accctgctac 1980
tggagatgtg gtctctggct acagtgcacg atctgtattg acaattgctt ttcaatttcc 2040
attcgaggac aatcttcagg ataatgtagc taccatggcg cgccagtatg ttcgcagtgt 2100
ggtttcatct gtccaacggg ttgccatggc aatatctccc gcaggagtga attcaacatt 2160
cgggtccaag ctttctccag gctcccctga agctgtaact ttgtcgcact ggatctgcca 2220
gagctacagt tatcacatgg ggacagagtt gcttcaagct gattcgaggg gcgatgaatc 2280
agtgctaaag aatctttggc aacatcagga tgctattttg tgctgctcat tgaagtcgct 2340
gccggttttc atttttgcta ataaggctgg gcttgatatg ctggagacaa cattagttgc 2400
tttgcaagac attactctag ataggatatt tgacgaatct ggccggaaag tgttgttcgc 2460
tgaatttccc aagatcatgg atcagggttt cgcgtacctg ccgggtggta tttgcatgtc 2520
tgcaatggga cgacatattt catatgaaca agctattgca tggaaagtct ttgcttctga 2580
agaaactagt gtccactgct tagccttctc atttattaac tggtcatttg tttaatgttg 2640
ctgtcaaatc tcctcttttt ttcctttttg ttttttgaca tcttcctcac agaggacact 2700
gacagacagg aacacagttg aacgga 2726
<210> 50
<211> 1730
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 50
aggttcttct tccttaatat tgagtcacag attagtacca ctactatagc caagaaaatg 60
tgaaatcata tagtactaaa tattaatttc agatgccaaa accataaatt tcccctcctc 120
catcattgaa aaccccctct gtcctttccc ctagagagac ccctttttcc tctctctctc 180
ctttctcttt ttattagacg catatattct ctcttctttc tctttctagg gttttcacct 240
gaaatagttt tatttcggtg atatgttagg atcctttggt tcatcatctc aatctcatga 300
tgaagaaact gatgatcaac ggcggagatt cagttccact tcccctgcaa tccaaatccg 360
gcaactactc attagctgcg cggagttaat ctcgcggtcc gatttctcgg ccgcaaacag 420
actcctcacc attttatcaa ctaactcttc cccttttggt gattcaactg aaagattagt 480
ccatcagttc actcgcgcac tttctcttcg cctcaaccgt tatatctctt cagccactaa 540
tttcttgaca ccatctaatg ttgttgaaag ttcaaatgat tcagctctac ttcagtcatc 600
ctatctttcc ctaaaccaag tgactccttt cattagattt agtcagctaa ctgctaatca 660
agcgattttg gaagctatta acgataacca acaagcgatc cacatcgttg attttgatat 720
taatcacggt gttcaatggc caccgttaat gcaagcacta gctgatcgtt accctcctcc 780
aactcttcgg attaccggta ctggaaatga ccttgatacc cttcgtagaa ccggagatcg 840
tttagctaaa tttgctcact ctttaggcct tagatttcag tttcaccctc ttttgattac 900
caataataat gacaatgatc atgacccttc aataatttct tctattgttc ttctccctga 960
tgagacatta gctatcaact gtgtatttta tcttcacagg ctcttgaaag accgcgaaaa 1020
gttaaggatt tttttgcata ggattaaatc catgaaccct aaagttgtaa cgctggccga 1080
gagagaagca aatcataatc acccactttt tttgcaaaga tttgtggagg ctttggatta 1140
ttatgcagct gtgtttgatt cattggaagc aactttgcca ccgagcagta gagagaggat 1200
gacagtggaa caagtttggt tcgggagaga aataattgat atagtagcag cagaaggaga 1260
taagagaaga gaaagacacg agagattcag atcatgggaa gtaatgttga ggagctgtgg 1320
atttagcaat gttgctttaa gcccttttgc actctcacaa gctaaacttc tcttgagact 1380
tcattaccca tctgaaggat accagcttag tgtttcgagt acgagtaatt ctttcttctt 1440
gggttggcaa aatcaacccc ttttttccat atcttcttgg cgttaaattt aaaaccctaa 1500
aaaacaagat ttttatctat ctgcatggtg aaggacaaag aggtcttcaa tctcaggttc 1560
tttttttttt ttttttttta tatatatatc ttgtttgggt ttaaggttat tgggctgatg 1620
aatgttttaa ttttaacata ggtctactta cgtagtagtt ataggttgat aatgagatat 1680
aattaactaa gtctttgtat aatgcagatc ctgaacttaa tctttatttg 1730
<210> 51
<211> 1827
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 51
aggttcttct tccttaatat tgagtcaaga ttagtactac tactatagcc aagaaaatgt 60
gaaatcatat agtactaact ttcccttctc cctagctact gataactcta attaatttca 120
gatgccaaaa ccataaattt cccctcctcc atcattgaaa acccctttgt cctttccccc 180
cagaccccct tttcctctct ctctctctcc tttctctttt tattagacgc atattctctc 240
ttctttctct ttctagggtt ttcacctgaa atagttttat ttcgttgata tgttaggatc 300
ctttggttca tcatctcaat ctcatgatga agaagctgat gatcaacggc ggagatgcag 360
ttccacttcc cctgcaatcc aaatccggca actactcatt agctgcgcgg agttaatctc 420
acggtccgat ttctcggcgg caaacagact cctcaccatt ttatcaacta actcttcccc 480
ttttggtgat tcaactgaaa gattagtcca tcagttcact cgcgcacttt ccattcgcct 540
caaccgctat atctcttcag ccactaattt cttgacacct aatgcatcat ctaatgttgt 600
tgaaagttca aatgattcag ctctacttca gtcatcctat ctttccctaa accaagtgac 660
cccttttatt agatttagtc agctaactgc taatcaagcg attttagaag ctattaacga 720
taaccaacaa gcgatccaca tcgttgattt tgatattaat cacggtgttc aatggccacc 780
gttaatgcaa gcactagctg atcgttaccc tcctccaact cttcggatta ccggtactgg 840
aaatgacctc gatacccttc gtagaaccgg agatcgttta gctaaatttg ctcactcttt 900
aggccttaga tttcagtttc accctctttt gatcaccaat aataatgaca atgatcatga 960
cccttcaatc atttcttcta ttgttcttct ccctgatgag acattagcaa tcaactgtgt 1020
attttatctt cacaggctct taaaagaccg cgaaatgtta aggatttttt tgcataggat 1080
taaatccatg aaccctaaag ttgtaacact ggccgagaga gaagcaaatc ataatcaccc 1140
actttttttg caaagatttg tggaggcttt ggattattat gcagctgtct ttgattcatt 1200
ggaagcaact ttgccgccga gcagtagaga gaggatgaca gtggagcaag tttggttcgg 1260
aagagaaatt atagatatag tagcagcaga aggagataag agaagagaaa gacacgagag 1320
attcagatca tgggaagtaa tgttgaggag ctgtggattt agcaatgttg ctttaagtcc 1380
ttttgcactt tcacaagcta aacttctctt gagacttcat tacccttctg aaggatacca 1440
gcttagtgtt tcgagtacga gtaattcttt cttcttgggt tggcaaaatc aacccctttt 1500
ttccatatct tcttggcgtt aaattataag ggaaattaaa accctaaaaa caagatttta 1560
tctatctgca tggtgaagga caaagaggtc ttcaatctca ggttcttttt gtttttttaa 1620
cttgtttgga tatgaggtta ttgagctgat gaatgtttta attttaacat aggcctactt 1680
acgtagtagt tataggttga taatgatata tatttaacta agtctttgta taatgcagat 1740
cctgaactta atttttattt ttattatttt gttgttaatg aaagattctg ttaccaaatt 1800
ttatcagtct atttaattag aggccaa 1827
<210> 52
<211> 649
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 52
gcatggacaa tctcatcttc tcaaacttca taaagatatc tttaaaaaaa agagaaaata 60
gaggtaatta gttgtatcaa tggatcaaca acattccact tgtttttctt cttcaagtaa 120
aattaatgac aaagaaaaga agaaaaaaag atcagttgtg aaactatcaa ctgatccaca 180
aagtgtagca gctcgtgaaa gaaggcatag aatcagtgat cgtttcaaga ttttgcagag 240
tttaatccct ggtggttcaa aaatggatac agttactatg ttagaagaag caattcacta 300
tgtcaaattt cttaagactc aaatatggct gcatcaaacc gtgattaata ttgtagatga 360
ttatgataat ccaaattatc atgatcagtt gctaatggct catgactcta attttgctaa 420
ttattatcct catgaaatgg tggaatattg cccagctcct gttgagaatg cacaaataaa 480
ttataacttg gaccagctgc agcttccagg ttatgcattt tcagatgggg atcaattcca 540
aggagaagaa actaatatta ctggtgattc ttttatgtac tattagttag ttaattatgt 600
tgcctaagtt taattagaat acgtagtgtg tggtagtatg gtatgttgt 649
<210> 53
<211> 740
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 53
aaacttcata aagatattat attaaaaaaa ataaagaaga agagaagata gaggtaatta 60
gctatagcaa tggatcaaca acattccact tgtttttctt cttcaagcaa aattaatgac 120
aaagaaaaga agaaaaaagg atcagttgtg aaactatcaa ctgatccaca aagtgtagca 180
gctcgtgaaa gaaggcatag aatcagtgat cgtttcaaga ttttgcagag tttagtccct 240
ggtggttcta aaatggacac agttacaatg ttagaagaag caattcacta tgtcaaattt 300
ctcaagatgc aaatatggct gcatcaaacc atgattaata ttgtagatga ttatgataat 360
ccaaattatc atcatcagtt gctaatggct catgactcta attttgctaa ttattatcct 420
catgagaata actcaactcc tgttgagaat gcacaaataa attataactt ggaccagctg 480
cagcttccag gttatgcatt ttcagatgga gatcaattcc aaggagaaga aactaatatt 540
tctggtgatg cttttatgta ctattaatta gtaattagtt aattatgttg cctaagttta 600
attagaatac gtagtgtgtg gtagtatggt atgttgtttt ctctctttct atctagcagc 660
ctaatgatgg gtttgtgtta attaattaga tgtagtaaat tgtaagtgtt ggttagttga 720
ttaagtatgt tgcaagtttg 740
<210> 54
<211> 15103
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 54
ttctagggat tacggaaatt catttacgta catttagttt gaataacttc ttaacaaaat 60
ggtaattgtt tttaaattct aaaaataaag gatagagaag gaaaatgtca cgacccgaaa 120
ttctcacctt cgggatcgtg acggtatcta acattttact tgctaggcaa gtcaacgttt 180
gagtaaatta tatgttattt caacaactca agtaattaaa ctcatttaaa ctgaaatgaa 240
actaaaaaga agtgtgaggt gacataataa cccaaaatat ttaagtacaa tccggacctg 300
gagttacaag tacatgagct actagaaatt ctacaaacag agtctgcaat aaatgtaatc 360
gtctcgaata aaatgaacag taactaagga aagaggaagc ggacttcaag gtctgcggac 420
gccatcagat ctaccccgag tctccgagta tgtcaatccg agccgataca gctcacgtag 480
tgctgggact agttctaaaa tctgcacaag aagtttagag tagtatgagt acaaccgacc 540
caatgtccat aagtgtcgag cctaacctca acgaggtagt gacgaggcta taacaggaca 600
ctcacgtaat tcaacatgta cgaactaaga catatacaca atatagtaac aataaagaat 660
taatctatga aattgggagg ggacatgcaa acggggaaca agatacgata attacaacat 720
aaataacaac ataagacagt caaataaatc atcaaccaag aaaagggata aacatgatga 780
ataaagatgg cacaacattg cccatcgtgc ttttactctc aaccttgcca tgaaataata 840
ataatagcac gacatcaccc ttcgtgtttt tactctcaat ctctccatga acaataaata 900
cggcacgaca tcactcttcg tgctttaact ctctttctca ccatgtatta atcagtaatt 960
gaaacaaata ataacacgac atcacccttc gtgctttaac actctccctt accatgtagt 1020
aatgaatata aaaataaaca agaataaacc ttacgtcaat aatgatttcg aaacacaaat 1080
cctcaatttc aaagaaatac tcaactatca caacttaatt cataaatgca gggagaacaa 1140
tcaaataaga aataaactaa tctaagcatg gacatcatga gtaaagaagg caataaatta 1200
caagcagcaa gtatcactcg catgttttaa cccaataata acacataagt actcgtcacc 1260
tcacttatat gttataccca aacatttaaa catatagcaa ataggcaaac aagtcctaat 1320
cactcaagtc aaggttagcg acgacactta cccacttcat agtccactca gagctctatc 1380
gaggcatttc ctctagaatc cgcctccaac ccactcgtat ctaaccacaa tcgactcgat 1440
aacgtcaaat aatgttaaga aaatcaatta cattacataa agaaaggatt tttacacctt 1500
ttcccaaaag taaaaaaaag tcaacctcga gctcgcttag tcaaaatccg agagtccgac 1560
caaaactcat ttatccattc accccgagtc cgattatgta attaattttg aaattcgaac 1620
tcaaattaag gtctaaatcc cgtatttgca aaaatccaaa attcttccta aatccctagt 1680
tttctactat ggaagaacaa agtttagggc tagaaattaa tgggcgatgt tacaaattga 1740
agaaaatgag ttaaagtgta caaacctatg aattggtatt gggttttcca ttcaaaattg 1800
catctaggcc gagctctaat ggagagtttt atgaaaaagg gtgaaatcct gtttttgaaa 1860
catttaaatc actgggcgtc aggtgttcat cacgatcgcg tgaaacttga cgcgttcgcg 1920
gagagcattg cctaactggc ttatacgatc gcgagtatct ccacacgttc gtgaaggttt 1980
agcctgcctc tcctccgcat tcgcgagcct aggttcgctt tcgcatagag taacctcaac 2040
tcctagccca gcctatctaa cactacgcgt tcgcgaagag cagacccccc agtgctccgc 2100
gttggcaacc aaggtctcgc gttcgggtag aataaaatcc tccccaatcc cagattccct 2160
ttcgcgaccg cgagaaaggc ttcgctgtct cgaagcacaa tacactagat gacagctgaa 2220
cctcgaaacc catatttttc taagttcaaa tggtccgtag gctatccaaa attcacccaa 2280
gccctagggg ctccaaacca aatatgcacg caagtacaaa aatattatac gaactcgctc 2340
gcacgatcaa agctccaaat aatacttaga actacgaatc aaacaccaaa tcgaatgaaa 2400
ttttcaataa agcttaagaa cattcaattt ttcaatcgga cgtccgaatc acgtcaaatc 2460
atttccgttt tgcaccaaat tttacagaca agtcataaat atagtaatga acatatacca 2520
agtttcggaa ctaagatccg gacccggtat caacaaagtc aaacatcggt caaatttata 2580
aagtctttaa acctttaaat ttcaaattct cgacaaattg cgataactcg agctagggat 2640
atccgaattc aattccgggc atacgctcaa gtcccaaatc acgatacaaa ctatcgggat 2700
cgtcaaaata cgaatccggt tctgtttcct taaaatattg gccgaaatca attcaaatga 2760
attttaaagc acgaattcac attttaatta atttttcaca tataagcctt cgggaagtat 2820
gaacttaaca cgcaaatcga cctgtcgggt tatcacagaa aaaaccatga gtaccaccag 2880
tagagttctc ttttaacttc tttttcctta gagtttagct ctgagttaaa aaaaatcttc 2940
ttgataatag taaccatata tagaataaaa atgtcctata atttcaaggg cagatatagg 3000
atacatgaat tatgaattca atcaaataca ataatatgtt caaatatcca attatcattg 3060
aattcagcgt tattactatc cacaaaattc gaactttaaa tatacttttg cataatttag 3120
attcaactaa acaaagatca tataagaaca aagatgtcct gtgatttcag ggcagatatg 3180
cgacataagg tatgaattca gtcaaattca atatttttag tatcagatgt acaaatatta 3240
aattctaatc tagtaactca aatggtcatt aaaatttagt ggtagtccaa ccacaaaatt 3300
gaaattctaa atctcctttt atataatgta gatcaagtta agaaatttca tctagacaat 3360
attgaccata tttggacaaa gatgtccttt gaacttttcc ttttatgttt atagctagaa 3420
aaaataatcc aaacagcggt gatcacatat tttattaaaa agaaaaagaa aaagaagttg 3480
tattattata ctctatcgga ttactgaata ttttatattc gtacattata atttcaatta 3540
aatagttgaa gagaaaggac ataaaagaaa acagaaaaac acaaaggcaa gagcaacaac 3600
agcagcataa aaacactggc attttcgatt tgcgagctca taaagcttta actcaagcaa 3660
attctctctc tctctctctc tctctctctc tctctctctc tctctccatt ttctttttct 3720
cttttctcac ccaccactct cacacacctc ttcacctcac cttacacact aaaaaaacat 3780
cactcctctc tctaaaaaat tcaatctttt tgctgttcca acatgtcttt tagagtttgt 3840
ttcagtttca gatcttaagg gcgggagtgt tatgcttctt ctaatatttt gaagctcaag 3900
aaaacagagc aaatttttgc tttcttttct cctacttttt gtggggggta attcttgttt 3960
ttgtaatctc aaagctggct gttttatgta tatactgaag gggttgtggt gatttgtttg 4020
tctactttaa gaaggtgcca tctttttcag taatatttgg gtaaaagttc tctctttttt 4080
ggccttaaac gcgaagattc aggcctctct caacgtgtca tttgttctct gtattaaaca 4140
cagctggaga attaattaca tagaggtaaa aaaaggggtt aaagattcca aagaattgaa 4200
aaaaaacaga gggctgaggt aaaaagttga tggtttttaa aaaaaaataa aagcttaaat 4260
gatgataaag tttggagctt tatgtgaatg gaaatggtgt tgtgtttgta tcaaacacga 4320
gtagtttaca gcttatgtga atttgaaaga gagagaattt ttgtctgtat ttatatcctt 4380
ttcagccata tctttcgtta gagcagtttt ggctgtacct taatttgtaa gggtttaagc 4440
gtgaagtgtg tgtttgagcc ttctgttata aggggcacaa agtatagaaa caacaaaagg 4500
ggcacctagg aatcttctgg ctcaatcaag atcgttcatt taatcttgtc tgagatcact 4560
agaaaaagaa aaaggaaaga taaagataaa gtctttgttt cagagaatct tagttctctg 4620
tgttgatata tataataaaa gctgtttgca gggaatatat ctacttgggg gtgtttttat 4680
ttcttttaag ggtgtttgaa aatttggaaa tcttgattat ttttttgttt gggattttgg 4740
ggtttgaggg caaatggcta tggtggtaca gcagcatagg gagagtagta gtggtagtat 4800
tacaaaacat cttgacagta gtggaaagta tgtccggtat acagctgagc aagtggaggc 4860
attagagagg gtttatgcag agtgccctaa acctagctcg ttgcgccgcc agcaattgat 4920
ccgcgaatgc cctattctgt cgaatatcga gcctaagcag atcaaagttt ggtttcaaaa 4980
cagaaggtac actgcccgct gttcaatttt gattgctcca atttggtttc ttttttgttc 5040
ttaaatgcat atatttaggt gtcgtgcact tgtgatcttg gactgaaata tgggataagt 5100
tagatgagtg atggttaaat tggaatatat cactgtgctt ctagtttcct aggcttgtcg 5160
attgggttgt atggattaat cggggggggg gggcattaag tgaatcgtga attggatgtg 5220
tagtttgatt tctgtctgtc gggtagttga gcttagattt tggaattgag ggtgaacatt 5280
gtgccatttc aggtgtcgag agaagcaaag gaaagagtct tctagactac agactgtaaa 5340
tagaaagctg tctgcaatga ataaactatt aatggaggag aatgatcgct tgcaaaaaca 5400
ggtttcacag cttgtgtgtg aaaatggctt tatgcggcaa caattgcata ctgtaagtta 5460
acataatttt tcctttatac ttgtggtaaa aagctttatt ttttgcttac tgtagacgaa 5520
tggtaacgta tatcttgtct tttgtttctg atgaaatggc taagcactat gaattttaag 5580
atttctgata ttccacagct tatggtaaca tattttaaac agtgtaaata aactttattc 5640
tgatgacact gttttaggac attcttatag ttatggaatg catggcttta gatatgggac 5700
taaattttat gttcatcgtg tttttgcatt ctatattctt ctactcgccc ttgttttgct 5760
gtgaagttga accaataaac aagaaacaga tgatggatat ctccggtgat cttttgttcc 5820
ataggattaa ttagactgta tttgtgtttt ctgcaggcat cagcggccac tactgatgta 5880
agttgtgagt cagtggttac cacccctcag cattccctca gagatgctaa caaccctgct 5940
gggtaattaa tttcaaacac ctatttctcc catcctttcc gtctatggtg tccattctcc 6000
aacatattta tgttatttat tcaatggcat atacaacatt ttgaggggct aatttgttta 6060
tctctaagtc aagtttgttc tctatgcaga ctgctgtcga ttgcagagga aaccttagca 6120
gagttccttt ccaaggctac aggaactgct gttgattggg tcccgatgcc tgggatgaag 6180
gtttgaactt tagtcaatcc tctttatttt ttgaaaattc agtattgcca tgtctctttg 6240
actggatagc taaaaaacta aattttcatt ctattgccag cctggtccgg attcagttgg 6300
gatttttgcc atctcacaca gttgtagtgg agtggcagcc cgagcatgtg gtcttgttag 6360
tttagagccg acaaaggtaa gcagtcatgt ggaaaattaa tttaaatgta gtgctgttgc 6420
tctattacta gttttggtcc tttgacgggt gtactagatg ttgccagttt cttcttagta 6480
aatatatttt tgtcaaatat ttacagattg ctgagatcct caaagatcga ccatcttggt 6540
tccgagactg ccggaacgtt gaagttttca cgatgttttc tgcaggaaat ggaacaattg 6600
agcttttgta cacgcaggta attaattacc ttctcatcaa tcttcacgta ggcttctgat 6660
tggagaagct acagcattga ggggattttt gaaatcattt cttttcagat atatgctcct 6720
accaccttgg ctcctgcacg tgatttttgg actctgagat acacaaccac cctggagaat 6780
ggtagttttg tggtatgcac atcctccgca ttagcgtgtc ttaggataag caatctggcc 6840
acttttgtac ttagttatga atattttgct gatagtttgt tgtatgtgcc atcaattcct 6900
cctcccctca aggtttgtga aagatccctc tctggtactg gagctgggcc gaatgctgct 6960
tctgcttccc agtttgtaag agctcaaatg cttccgtccg gatatctaat ccgaccgtgt 7020
gacggtggag gatccattat acatattgtt gaccatctga atcttgaggt cagattgcac 7080
actgtactac cacttccctt tctttttaac ttgttctgtc ttgcagctgg acttcacggc 7140
ataatgtttt tcttcaggca tggagtgccc ctgagatttt gcgtccactt tatgaatcgt 7200
caaaagttgt ggcacagaaa atgactattg cggtgagttg aaccgttgat tgtcattaaa 7260
tactggatgt gtaacaacct ttttagtctt cacaactaga tctcaatttt tgttgagctc 7320
tgaagtcgaa agggttgtaa tttctggacg agcagttaga tatagcctga tatttttgtt 7380
tattcagtta gaagttccca gctttaaaaa tatagaacac ctgacaaatc cttagtctct 7440
taatgcacgt tattgaggat ttctttgttt tttcgagttt tctaaggttc attattgttt 7500
tcctcatggg gttgccataa aagtctgcat gtgaaacata tagtattgaa gaactgtagg 7560
ctgtgaagcg caccatactc ttaactgcat tagttgttgc tttaattcca tatgttgctc 7620
tgagaatact tgcagcattt tttatgtttc aagtacttga gcaattaccg tagcttacca 7680
tcacaacaaa agaaatacta attatagtat gtttttgctg taaaggcact gcgatatgca 7740
aggcaaatag ctcaggagac tagtggggag gttgtatatg gtctgggaag gcaacctgca 7800
gttcttcgaa catttagcca gagattaagc aggtgctgtt tattgctctg attgttctgt 7860
gctatgagat atgatatgcc ataaaagtag acatacgaat tctgaagcac aagtatcata 7920
attaagctat tttctatatt gcagaggctt caatgacgcc atcaatggat tcagtgatga 7980
tggctggtca ttgttaagtt ctgatggtgg tgaagatgtt atagttgctg tcaattcaag 8040
gaagaacatt gccaccactt ccgttcctct ttcaccgctg ggaggcatcc tttgtgccaa 8100
agcatcaatg ctactccagg tgaatagatt accttttaac tgactagaaa ttttcattgg 8160
ccaactacct ttgccttgtt agataaaatt gttccagact gttgcagatt ttgatgatgc 8220
tttcaatttc taaactcttg gaatgaatcg ggattcctgg aatataagag aatattactc 8280
agtgttctat aaagctattt gtttaatgca ccatgtgggg catcttgttg ctattaaatg 8340
gaagaatgag aattgacttt taactcttct gtatggtggc agaatgttcc tcctgtggta 8400
ctggttcgat ttctcaggga gcaccgttca gagtgggcgg actttaatgt tgatgcctat 8460
gtagcttcgt caatgaaatc ttgttcatat gcatatcctg ggatgaggcc taccagattt 8520
accggaagtc agataataat gccacttggc catacaattg aacatgaaga ggtaagcact 8580
ttgcacttgc cccagttcca tccatcccat gtgttggagt gtgcttatac agcaccagta 8640
ttttttataa tcagaaagtt agcactcttt gaattgctag gcttgttacc taatattgct 8700
aatattatac tttagacttc ctctcatttt ttttttattt tgttttgctt tgcagatgct 8760
tgaggttatt agattggaag gacactctat tggccaggaa gatactttta tgccaagaga 8820
tgttcacctt ctccaggtac cttttgccta tgcattgatg tttcggtgtg ttatctacgt 8880
acagacattg ttgaagcaat agctaacaaa cggttatttc tagatgtgta gtggaactga 8940
tgagaatgct gtcggagctt gttctgaact agtttttgct gcaattgatg agatgtttcc 9000
agatgatgca cccctgttgc cctccgggtt tcgtatcatt cctctcgagt caaaatcagt 9060
tgagtaaaaa tatttcattt tcaactttaa gcattgaatt tggccaatct attgtttaca 9120
tggattattt ttcattttgc ttgattttgg agcataaccg gtgattctat tttcagagcg 9180
atccccagga tacatcgaat gctcatagaa cactggatct ggcatcaagt cttgaagttg 9240
gcccagcaac aaaccctgct actggagatg tggtctctgg ctacagtgca cgatctgtgt 9300
tgacaattgc ttttcaattt ccattcgagg acaatcttca ggacaatgta gctaccatgg 9360
cgcgccagta tgttcgcagt gtggtttcat ctgtccaacg ggttgccatg gcaatatctc 9420
ccgcaggagt gaattcaaca ttcgggtcca agctttctcc aggctcccct gaagctgtaa 9480
cgttgtcgca ctggatctgc cagagctaca ggtaaaatga tttctcaact atggtgaaac 9540
cttgttctct tcgtttcagc tcaatatggg gtttattgct ttacatgttc atactgtcgt 9600
gcttacaagt cactcgttgc aaatctcatt taccaccaag agccaaagta gtgtcaagtg 9660
tgcatgttga gatcttcaat tattttatga gaatttttcc tttctcaaca tattgagaaa 9720
aagcagacgg tcttagaagt acttttctga ttgttaacat accgttttct tcttttgcat 9780
ttaatatcca gttatcacat ggggacagag ttgcttcaaa ctgattcgag gggcgatgaa 9840
tcagtgctaa aaaatctttg gcaacatcag gatgctattt tgtgctgctc attgaaggta 9900
tgaattctct tatcatgtaa acagcatgtt acggttagta aaaaaatatt gtatgttgtg 9960
ttgcggtgaa acatgaacat atacgtaaag aaaaaatgta ttaacctagt aaatccacga 10020
tgaaggcaga tttgttcaaa agttaatctc atgaccctaa ttaatattag aatacgaaag 10080
agctggacaa ggatattaga aataagtccg acttaaatta tacttgtgat ggtgatattt 10140
tatggtgaaa atgccatatc atgggtgtat atttgaacta cttgtgattg gcattttgat 10200
tgtcctcatt ttggtcccta gcatgctttt gacatgtcaa catggaaatg agttgctaag 10260
aaattggaag gactgaccta ttcgttcacg ttccttttat cttgttaaaa gaatgtgttt 10320
agtaagttaa atttctttct ctgctgttgc agtccctgcc ggttttcatt tttgctaata 10380
aggctgggct tgatatgctg gagacaacct tagttgcttt acaggacatt actctagata 10440
agatatttga tgaatctggc cggaaagtgt tgttcgctga atttcccaag atcatggaac 10500
aggtatttac agctgactct ggtcttttgc agaacctaga aaacaaaagt tgaggtctta 10560
actgttactt ttttccgcga tgttgattct tgatcatagg gttttgcgta cttgccgggt 10620
ggtatttgca tgtcagcaat gggacgacat atttcatatg aacaagctat tgcatggaaa 10680
gtctttgctt ctgaagaaac tgtccactgc ttagccttct catttattaa ctggtcattt 10740
gtttaatgtt gctgtcaaat ctcctttctt ttttttcctt tttgtttttt gacatcttcc 10800
tcacagagga cactgacagc caggaacaca gttgaacgga atgatctttg ggacggatga 10860
aaattttgta acttgggggg ctcccgtctg ttttaccttt aatttaatta gactaaattt 10920
gtattttgct tcctgaattc ttcatactct tatgtaaatt ttctagtgca gcttttttga 10980
gtgcagatgt ttgtttccgc atattctacc actgattcat tttatattta gctttagtat 11040
ttgcagtgat attcaagatg ctgcaatgtt gctaagctta tgtgatattt ttttcttata 11100
acaactgaga aaacttattt cgacaactta acttgaacgg aaatcctaca ttattaaaca 11160
aagagtagat aaaggttagt cgtgaatgga agataaatta ttataatata tacaacaaag 11220
ataagtacaa aataatgatg gtctcgattg ttattttaaa ataaatttta ggtttaatgt 11280
gtgcttttgg acttgaataa caagctcaag atgtgctagg gagattgcct ccaatgaata 11340
agacctgtct tatcatccat tatatgctat gtaatgtcaa agattcaagg gtgcccacta 11400
tacttttgca caatcctgca catatagcaa cagccaacaa gcatagcaca actcaagttt 11460
gcgcattgac ctgctttaca caatttgtat ttacacactg cagcggcaat tgtatgtttt 11520
ttggacatgg atcatccaga caaacatctg caatatcaga acaaaaatca gttctttaca 11580
aaaatgtttg gatattgttt gagtatttcc tgagaaccat tcttcctctt caccgagatg 11640
caagtgacca aaagtttaag agaaatgatg gcatagcaca gaaccagcta agaaaggcca 11700
tggcagtagc agtctcaaac tctgtgcagt gattctgaga gcagacacca aggtcattgc 11760
caatcagtac agtaatgcca gcagaagcac aggctgcagc aaacgtgagg gtagaagtga 11820
cctgcaagaa agatagtatt tataaggaga tgaatggaaa ggagtcatgt agctactcag 11880
atgcttcagg atgagcttat atacaacttg tgttaaatgc tcaaagcgga atgtggtgca 11940
cgcatagccc gaaaagttta taataatgga gtaaaaggaa gagaccaatt caagaagtgc 12000
aaaatggaga taggtacggg ggatttgctg ttataataaa ttacaaatct ggttatcaag 12060
aaacaataaa ctattaactc cagactatga gatatttaaa agataataga gcttttctac 12120
ctacaaggta atttctacat catattagaa gggttgagga accaggttaa aaaaaccaat 12180
tttatgtctg acctcacaga ccttgaggga tcagcgatgg atcagaaatg cccaagaata 12240
catcaaaaag tagcaaaatc tcctacaaaa tgaacagact gaggaagaag ttcccaccag 12300
acgggtaact gggctaagag ggctcaaaat ctgtttcagt tattgcacgg aagttgtcca 12360
actgtgacta agtcttacat aaactgtgtc actctcttcc atcattaggg acgaatgatc 12420
tggatcccca gccattcata tcataactct ttcatgcaag agtttatcac tctattcata 12480
attaattttg cagtcaattt tattccgaaa agaaacatag caagactata ttaatattaa 12540
ccaatttaaa gtcccaaatg agccatgaca tatatcaata ggcatattaa acttggagag 12600
aacgaaagag ggcatgctgc caaagtttat atcaataggc atattaaact tggagcgaaa 12660
acgaaaaaag ggaatgttgc caaagtttac ttgcaaagag gaaattttag gggaaaagtc 12720
atatttacta attctctaat accatttcaa ttcactttta aacaattact atgagtagcc 12780
caaatcattg attatgttta actgcgcatc tttcatatct ttctgagctc cttaaataaa 12840
tggctattgc ctattgcaaa attcatatac cacaagtata cgaccaactt tactggtgca 12900
gtaaaatact gcttatccgg ctcttaaatg taaaaaaggc atgttgctta taaggcaacc 12960
aagagaatgt gaccatctta aacgtaaagg gtacctaatt ttaatatttc attgctgagt 13020
atacaataaa aaaattaggc aactcaactt ctctattttt tttttctgta taaactctat 13080
ggatacacca tcttactccc ttcttccact atccttttta gtgcaccccc aaaagattga 13140
aacatttcta tttatgaaaa ctacctccca acactatcct tttcactaaa aaagaaaatt 13200
ttaatgctca tttctaccaa gagcgagcca tcagcattca tgatgaagta tatcatgata 13260
gaaaggccat tttcgcaact tgtgtttgtt ttacttatgt gtgtatacat ttctaaaatt 13320
cactttccaa taaaaccacc tcataatatg gaacggacgg agtactaaaa gtcataagtt 13380
tataggaatt ccaagttcat gaaacaccct taacagctag ccaagttatc aaaggctatg 13440
ccctacttct tgagttgtca cccgcgtttt atgagtttct catagttgca tcatcgcggt 13500
gcctagaggg gatatgtacc aacttaatct taccaattag tctaaagaaa ataatttgat 13560
atgtatttgt gcaagacgtt gaagcaagct ggtagaggtt gtattcaacg gcacaaaaac 13620
caatgaagcc ccttaatttt ttgaatccaa tataatactt ttagaatcct tataaaaaaa 13680
atgtttagaa tgccattact agaatttagt aagcatacca tgaggtctct aagaaattaa 13740
tcatctctct catggcatta tatattacac aaccttccct taaattttgg tacctatacg 13800
agaccctctg gatgggtcaa aaactgaata actaaagaaa caacaaagaa ataggcatta 13860
ctttttctta ttttacctta taaaatagaa ataattaatt ttcaaacttt atgccataga 13920
ggatgcccta tccatcactg gtaactactc gtgagaccaa gcaagtggaa taatccgaag 13980
aaagaggggt gagggagact cctaattgtt tcccccattt agatcctgta aatgcaattt 14040
aattccctca tcctatttgt ttgctccatt aagatattat taataaacac attttaataa 14100
aattagtaga gatgcatatt tccaagtgat tgttttgtat taaccaaacg taataaataa 14160
ggatatttac aaaaactcta gtacatattg caagatccat tcaatgggat ggaagaagca 14220
tgcagatcac ttcacaaact attgacatag ttttttatta tctggattga tcatatttgc 14280
aaatagttta caaagtttta agattaaatg cattaaattg gtataatata taggatctaa 14340
agtcattcaa ttcactagaa aactcgtgaa attgtgacct tggaccttaa atagcaacag 14400
aagaatgaaa ggccagcgga aattctgtta aatacatggt cattatggac aagagaaaat 14460
gggtatggtc attgcacaga attactccat gagtagatgt gaatattgac aatattgaat 14520
tgtgtttaat ccagcacaag ctgccccact cttgagctag tcaattttcc ggcacttctt 14580
ccacatacat aagagttgaa aaccaaaaat tggccctgga taacttatat ccaagcttca 14640
tatttcaaag actactaggt aggcaattag ggcataatat ctctggaaac ctagccaatt 14700
tctttgttaa aaagaaaaat gggtaaataa aaataatcaa acattctaaa caactgaaga 14760
gtagttaaac aaaggtcatt ttgcataaac aggaagttag tgcatgcata atatacaagc 14820
tatctcaatt aaaactggat aagacataaa gaaaaatcat atcgatccaa gtatatatgt 14880
attaaaattt tcagtgcaat tgcaaggtgg aactaaacca gaaacacata agcaaaagtc 14940
atatctttcc atgccaatcg caagctggaa agttttttaa ttccatatta tacttatcaa 15000
acgaaagaaa gtcacgacac tcatctattt gatcaattgc aatagctatc ttttgtatca 15060
agtccttgca cgtctgattg tatcagtctc tattcaaaag cta 15103
<210> 55
<211> 14465
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 55
agattttggc tattcttttt tgaaaatatt gtttgttcat gaaatatgat cacttttgat 60
tcaatttcaa gttgcagttt ttgggtgaaa attttctttc actcacaaaa atttaactct 120
ttttcaaatt aaatgcatat caaacacaac ttcaacttcc aaaagttatt ttcaccataa 180
cttcaaaaac tattttatca agttttgaca aaatctatat ccaaacgcta gcgaattgtt 240
tttacccaag aactgtccta cataagtttt tgaaaactcg aaatttctct tcaatttttt 300
tttaaaattg atcatattcc atgaatatac aatattttta attttttttt taaatagcca 360
aaagtatatg accaaccggg agctaaagtt taggttaaac aatcaaatgg ttaaaagact 420
agcataacaa tttagtcatg taggtgattt gctagtcatt ttcaaaatgt tacatttcct 480
agactggagc aggagcaaag attcaagaaa ttaatcctaa tactgacaca aatattagat 540
gactagtgtg aattgatctc atagggggat catccattca cttgctacct tactagccat 600
cagttaaaac aacgaaaaga ttgatacaga ttctcacaaa tgaagaaaaa aagaagtaaa 660
ctacaaggac aaaaaacaaa gatttaaagc ccatttggat ggtcttaaag atttgactgt 720
tatttttggt tcgttttaag taattttcaa cttatttcaa actctttata attttgagcc 780
cgttaggatt aactgattta aactagctga taagcattta gtgctgaaaa atatttttaa 840
gtgttgaaac tgatttaata aataagcagt tacatgtttg attacaagtg ctgaaattga 900
taataagttg ctgcaatatt tgataaaaaa aatgccgata aattcctttt ttgttaaaat 960
gacttaaata accttagaag tgtttacact tataaggacg taatttcttc aaaattttaa 1020
attccaaatc gatccaaata caaaatattc tatttgtcat ttttttaaaa tacaactgtg 1080
cttagataag ataactttta tgataaatat aatttatttt atgctaagta tataatcata 1140
agttataata attaataaat tgataaaaaa ttttcaataa aaaataaacc taaataggac 1200
aaaatatttg aagagaaaca aacactgtgt ttatcaccac aacacttaga aagcaacaca 1260
cgtcctcatt tattacatac aattcaaaga ttaatacaca atcaatagat ataaatatgc 1320
aaaggaataa ataatttgct tatcttaaat agtcaatgag cattgtaggc ttgaagaggc 1380
gggggagggg ggtttaggtt gaaatagtac ttgagtcagt gagtatccat gtaagagttt 1440
tgttctaaaa aaaaatattt taaggataaa atagtaaaat ttttgatcaa atttaaagtg 1500
cttataagct aaaaatttat aagctgaggg tgaccgactt atgatttatt tttgacttat 1560
aagcacttga cttataaaca cttttaactt taccaaacgc gtaaatatac cgataaatgc 1620
ttataagcta gtttgacgag cttataaact taaccgaaca ccctcttact tatataaaaa 1680
taaaagaaaa tattaaaagt aataaatctt tttgcagaga gtagagtaga ggggtgaaac 1740
cctaatagtc agagagacag agtatagacc acaaaggggc acagtagcca taggtattgg 1800
cagaagaaaa gattatgatt tgaccaaagc atttatatcc acagtacatg gcgtgggtct 1860
tatatgtcat ttcatatact gtacattgga ctagcttgta aatgcgccga atgggtaata 1920
ttaaattttt tgtttttata ataataatgg ctaaatcaat ttattattta tcaactaatt 1980
tcacggtata tatatgttat cacaaaataa ataactttat attgacactt catagttttt 2040
gataattatt aatttttaat tatcaactag aaatattact gagaaaagaa tccagacagt 2100
agagatcata aatattaaca aagttctatt agtatcttat aaatgaggct taaatctaag 2160
tcaaaacaca agagacgttt gcaaatgtgc caacttatca aattatgaat cacatttcac 2220
aatctgcctg ataatccctt tgaatttaca ttaatacatg ctccaaaaaa tttaacttta 2280
ctcactttaa cttaagaaca ttggctttat tcctctaatt tgtgaatata caagcagctt 2340
gttctagaca ttactgaaat tcatttacgt acatttagtt tgaataacta tttaacaaat 2400
ggtaattgtt tttaaattct aaaaataaag gatagagaag gaaaaaacca tgagtaccac 2460
ccgtagagtt ctcttttaac ttctttttcc ttagagttta gctctgagtt aaaaaaattc 2520
atcttgataa tagtaaccat atatggaata aaaatgtcct ataatttcaa gggcagatat 2580
acgatatata taaattatga attcaatcaa atccaattgt atgttcaaat attcaattat 2640
cattgaattt agcattattg ctatccacaa aattcaaact ctagatatgc tttgatataa 2700
tttagattca tctagacaat agagaccata tagaaaaaaa aaatcctgta atttgaggga 2760
cagatatgcc atgtaagtta cgaattcagt caaattcaat aatttagtat cagatgtaca 2820
tatattaaat tctaatttag taactcaaat gatcattaaa tttagtggta ttccaaccac 2880
aaaatttaaa ttctaaatct ccttttatat ataatgtaga tcaagttaaa acattcatca 2940
agacaatatt gaccatatat ggacaaaaat gtcctttgaa cttttccctt ttatgtttat 3000
agctagaatt catgtttata gctagaaaaa atcatccaaa cagtggtgat cacatatttt 3060
attttattaa aaggaaaaag aaaaagaaaa agaagttgta ttattatact ctagcagata 3120
actgaatatt tatattcgta cgtaccatat tgcaatttta attaaattat tagtgaagaa 3180
aaaggacata aaataaaaga aaacagaaaa acacaaaggc aagagcaaca gcagcagcat 3240
aaaaacactg gcattttcga tttgcgagct cataaagctt taagtcaagc aaattcccac 3300
atcacagtct ctctctctct ctccattttc ttttgccctt ttctcaccca ccactctcac 3360
acacctcttc acctcacctt acacacacta aaaaaacatc acttctctct gtctctctct 3420
ctaaaaaaaa ttctatcttt ttgctgttcc aacatgtctt ttagagtttg tttcagtttc 3480
agatcttaag tgggaggtgt tatgcttctt cttatatatt gaagctcaag aaaactaaga 3540
aaacagagca aatttttgct ttcttttctc ctactttttg tgggggtaat tcttgttttt 3600
gtaatctcaa agctggctat tttatgtata tactgaaggg gttgtggtga tttgtttgtc 3660
tactttaaga aggtgccatc tttttcagta atatttgggt aaaggttctc tttttttggc 3720
cttacacgcg aagattcagg cctctctgaa cgtgtcattt gttctctgta ttaaacacag 3780
ctggagaagt aattacatca aggtagaaaa aggggttaaa gattccaaag aattgagtgt 3840
ttgaaaaaaa aaacagaggg ctgaggtaaa aagttgatgg ttttaaaaaa aataaattaa 3900
atgatgatag agtttggagc tttatgtgaa tggaaatggt gttgtgtttt tatcaaacac 3960
gagtagttta cagcttatgt gaatttgaaa gagagagaga atttttgtct gtatttatat 4020
ccttttcagc catatctttc gttagagcag ttttggctgt accttaattt gtaagttttt 4080
aagcgtgaag tgtgtgtttg agccttctgt tataaggggc acaaagtata gaaacaacaa 4140
aagggggcac ctaggaatct tctggctcaa tcaagatcgt tcatttaatc ttgtctgaga 4200
tcactagaaa aagaaaaaaa aaagagataa agataaagtc tttgtttcag agaatcttag 4260
ttctctgtgt tgatatatat aataaaagct gtttgcaggg aatatatcta cttgggggtg 4320
tttttatttc ttaaaagggt gtttgaaaat ttggaaatct tgattttttt tttggtttgg 4380
gattttgagg tttgagggca atggctatgg ttgcacagca gcacagggag agtagtagtg 4440
gtagtattac aaaacatctt gacagtagtg gaaagtatgt ccggtataca gctgagcaag 4500
ttgaggcatt ggagagggtt tatgctgagt gccctaagcc tagctccttg cgccgccaac 4560
aattgatccg tgaatgccct attctgtcga atatcgagcc taagcagatc aaagtttggt 4620
ttcaaaacag aaggtacact gcccattgtt caatttggat tactccaatt tggtttcttt 4680
tttgttctta aatgcatata tttaggtgtg tactgcactt gtgatcttgg gctctagttt 4740
gtttggtact gctcaaatct tggattagtt agatcagtga tggatgaagt ggaatatatc 4800
actgtccttc tagtttccta ggcttgtcga ttgggttgta tgagttaacc gtggggcatt 4860
aagtgaatca tgaattgcat atgtagtttg atttctgtct gttgggtagt tgagcttaga 4920
ttttggaata gagggtgaat attgtatcat ttcaggtgtc gagagaagca aaggaaagag 4980
tcttctcgac tacagactgt aaatagaaag ctgtctgcaa tgaataaact attgatggag 5040
gagaatgatc gcttgcaaaa acaggtttcg cagcttgtgt gtgaaaatgg ctttatgcgg 5100
caacagttgc atactgtaag ttaacataat ttttccttta ttatttatgg taaaaaacct 5160
tttttttcac ttaacgtatc ttgtcttttg tttctgataa gcactatgga ttttaagatt 5220
cctgatattc cacagcttat ggtaacatat tttaaacagt gtaaattgtc tttattttga 5280
tgacaggttt taggtcattc ttatagttac gaaatgcatg actaaatttt gaattcatcg 5340
tgtttttgct ttctatattc ttctacccgc ccttcttgtt ttgctgtgat attgaaccaa 5400
tggacaagaa acggatggca gatatctccg gtgatctttt gttctgtagg aattaattag 5460
actgtatttg tgttttctgc aggcatcagc ggccactact gatgtaagtt gtgagtctgt 5520
ggtaactacc cctcagcatt ccctcagaga tgctaacaac cctgctgggt aattaatttc 5580
aaactcctat ttctcccacc ccttctgtct atggtgttta tacatattta tgttatttat 5640
taaatggcat agaccacatt ttgaggggct aatttgttta tctctaagtc aagtttgttc 5700
tctccgcaga ctgctgtcga ttgcagagga aaccttagca gagttccttt ccaaggctac 5760
aggaactgct gttgattggg tcccgatgcc tgggatgaag gtttgaactt tagtcaatcc 5820
ttttttgttt taaaaaaaaa ttcagtattg ccacgtgcct ctttgactgg atagctaaaa 5880
aactaaattt tcattctatt gtcagcctgg tccggattca gttgggattt ttgccatctc 5940
acacagttgt agtggagtgg cagcccgagc atgtggtctt gttagtttag agccgacaaa 6000
ggtaagcagt cttgtggaaa attaatttaa atgtagtgct gctgctctat tactagtttt 6060
ggtcccttga tgagtgtact agattatgcc agtttcttct aagtacatat atttttgtct 6120
aatatttaca gattgctgag atcctcaaag atcgatcttc ttggttccga gattgccgga 6180
acgttgaagt tttcacaatg ttttctgcag gaaatggaac aattgaactt ttgtacacgc 6240
aggtaattaa ttactttctc atcaatcttc acgtaggctt ctgattggag aagctacagc 6300
attgagggga ttgttgaaat catttttttt ccagatatat gctcctacca ccttggctcc 6360
tgcacgtgat ttttggactc tgagatacac aaccaccctg gagaatggta gctttgtggt 6420
aagcacatcc ttcacattag tgtgtcttag gattagcaat ctggccactt ttgtacttag 6480
ttatgaatat tttgctgata gtttgttgta tgtgcccatc aattcctcct ccccgtaagg 6540
tttgtgaaag atccctctct ggtactggag ctgggccgaa tgctgcttct gcttcccagt 6600
ttgtaagagc tcaaatgctt ccgtctggat atctaatccg accgtgtgac ggtggaggat 6660
ccattataca tattgttgac cacctgaatc ttgaggtcag attacacgct gtactaccac 6720
ttctctttct tattagcttg ttctgtcttg cagctggact tcactgcata atattgtttt 6780
tcaggcatgg agtgcccctg agattttgcg tccactttat gaatcgtcaa aagttgtggc 6840
acagaaaatg actattgcgg tgagttgaac ccttggtttt tattaactac tggatgttta 6900
acaacctttt tggtcttcac aactagatct caatttttgt tcagctctga agtagatagg 6960
attgtacttt ctggacgagc agttagatat agcctgatat ttttgtttat tctgttagaa 7020
gttcccagct ttaaaaatat agaacacctg acaaatcctt agtctcttaa tgcacgttat 7080
cgaggatttc ttcgttattc gagttttcaa aggttcatta ttgttttcct cattgtgttg 7140
ccataaaagt ctgcatgtga aacatataag taatgaagaa ccttatgctg tgaagcacag 7200
catactgtta actgcattcg atgttgctta attccagaag ttgctctgag aatacttaca 7260
gccttttttt atattttaag tacttgagca attaccgtta cttaccacaa cagcaaaaga 7320
aatactaatt atggttagtt tttgctgtaa aggcactgcg atatgcaagg caaatagctc 7380
aggagactag tggggaggtt gtatatggtc tgggaaggca acctgcagtt cttcgaacat 7440
ttagccagag attaagcagg tgctggttat tgctctgatt gttctgtgct tcgagatatg 7500
atatgccata aaagtagaca tacgaatcct gaagcgcaag tatcataatt aggctatttt 7560
ctatattgca gaggcttcaa tgatgccatc aatggattca gtgatgatgg ctggtcattg 7620
ttaagttctg atggtggtga agatgttata gttgctgtca attcaaggaa gaacattgcc 7680
accacttccg ttcctctttc accacttgga ggcatccttt gtgccaaagc atcaatgcta 7740
ctccaggtca acagattaag ctttcttgaa ctaactacag attttcattg gccaactacc 7800
tttgccttgt taattcactg aataggtcaa gtaattctaa agacaagttt tgcagtgctc 7860
ttgttgcctt gttagttcat agcaaacaga gttgcagctg ttcaaagtag gatcatatat 7920
tgtgatacct attcagtatc tgtattagat ctagtatcac aagacaagtt ttctttactg 7980
ctcttgtttc ttagaaattg gctctatact cttactaaaa aagagcgata atggtagatt 8040
ttgaagtcga ggaaaaatta aaatcgttcc ggattgttgc agatttttat tatgctttca 8100
atttctaatt ctaggaaaga atcaggattc ctggaatatt agagaatatt actcagtgtt 8160
ttataaagct atttgtttaa tgctctgagt agggcatctt gctattaatt ggaagaatga 8220
gaattgactt ttaactcttt tgttcggtgg cagaatgttc ctcctgcggt actggttcga 8280
tttctcaggg agcaccgttc agagtgggcg gactttaatg ttgatgccta tgtagcttcc 8340
tcaatgaaat cttgttcata tgcatatcct ggggtgaggc ctaccagatt taccggaagc 8400
cagataataa tgccactggg ccacacaata gaacatgaag aggtaagcgg tttgcaattg 8460
ccccagttct cacttatgtg ttatggggaa tgcctcgaca tacatgagca agaatttgag 8520
acttgagact tcctctcact ttattttggt ttgcagatgc ttgaagttat tagattggaa 8580
gggcactcta ttggccagga agatgctttt atgccgagag atattcacct tctccaggta 8640
cttttgctta tacattgatg tttcggtgtg ttgtatgtac atacattgtt gaaggataat 8700
gctaacaaac agttatttct agatgtgtag tggaaccgat gagaatgctg tcggagcttg 8760
ttctgaacta gtttttgctg caattgatga gatgtttcca gatgatgcac ccctgttgcc 8820
ctccgggttt cgtatcattc ctctcgagtc aaaatcagtt gagtaaaata ttttgatttt 8880
caacttcaag cattgaattt ggcaaatcta ttgtttacat ggattttttt ttttcttttc 8940
attttgctcg attttggagc ataaccggtg attctatttt cagagcgatc cccaggatac 9000
atcgaatgct catagaacac tggatctggc atcaagtctt gaagttggcc cagcaacaaa 9060
ccctgctact ggagatgtgg tctctggcta cagtgcacga tctgtattga caattgcttt 9120
tcaatttcca ttcgaggaca atcttcagga taatgtagct accatggcgc gccagtatgt 9180
tcgcagtgtg gtttcatctg tccaacgggt tgccatggca atatctcccg caggagtgaa 9240
ttcaacattc gggtccaagc tttctccagg ctcccctgaa gctgtaactt tgtcgcactg 9300
gatctgccag agctacaggt aaaatgattt ctcaactatg gtgaaacctt attctctgca 9360
tttcagctca atatggggtt tattgcttta catgttcata ctgtcgtgct tacaagtcga 9420
ttcattgcaa atctcattta ccaccaagag cggaagcagt gtcgagtgtg catgttgatc 9480
ttcaattatt ttttgagaat ttttcctttc tcaacatatt gagaaaaatc agatggtctt 9540
agaagtactt ttctgattgt taacataccg ttttcttctt ttgcatataa tatccagtta 9600
tcacatgggg acagagttgc ttcaagctga ttcgaggggc gatgaatcag tgctaaagaa 9660
tctttggcaa catcaggatg ctattttgtg ctgctcattg aaggtatgaa ttctcttatg 9720
aactcatgta aacagcatat tacggtttgt tagtaaaaaa attgtaggtt gtgttgcggt 9780
gaaacatgaa catatgcata aagaaaaatg tattaaccta gtagtgtcat gaccctaatt 9840
aatattagaa tatgaaggag ctggacaatg atattaagaa ataagctcga cttaaattat 9900
atttgtgatg gtgatttttt atggtgaaaa tgtcatatca tgggtgcata tttgaactac 9960
ttgtgattgg cattttgatt gtcctcattt tggtccctag catgcttttg acatgtcaac 10020
atgcattgct tttgacctat tcatccgcct tctagtcttt tatcttgtta aatgaatggc 10080
gttagtaagt tgaatttctt tctctgctgt tgcagtcgct gccggttttc atttttgcta 10140
ataaggctgg gcttgatatg ctggagacaa cattagttgc tttgcaagac attactctag 10200
ataggatatt tgacgaatct ggccggaaag tgttgttcgc tgaatttccc aagatcatgg 10260
atcaggtatt tacagccgac tcttagtctt tgcagaaccg agaaaccaaa gttgaggtct 10320
taactcttac tttcttcgat tctgtttatt cttgatcata gggtttcgcg tacctgccgg 10380
gtggtatttg catgtctgca atgggacgac atatttcata tgaacaagct attgcatgga 10440
aagtctttgc ttctgaagaa actagtgtcc actgcttagc cttctcattt attaactggt 10500
catttgttta atgttgctgt caaatctcct ctttttttcc tttttgtttt ttgacatctt 10560
cctcacagag gacactgaca gacaggaaca cagttgaacg gaaagatctt gggaccgatg 10620
aaaatttttg taacttgtgg ggctcctgtc tgttttgcct taatttaatt agactaaatt 10680
tgtattttgc ttcccggatt cttcatactc ttgtgtaaat ttactagtgc agcttttttg 10740
agtgcagatg tttgtttcca aatattcgtt cactgaatca tttcaattta gctctagtat 10800
ttgcagtgag aatcaaaagt ttgtgatact tttttggtat atcagcagag aaaacttctt 10860
tcagcaactt atcaatttga ttggcaatcc tatacgacta aatagactcg ataaaggctg 10920
gttgtgtgaa tgtaagataa taaattatat atactacagc aagagttaag gatgaagtac 10980
aaaataatga cagcctcttc gattgttatt ttgaaacaag ttttaggtga aatatgtgtg 11040
cttttggact tgaatctaaa tgttacgttt gaataacgtc ggaacaaact ccaaatgtgc 11100
taggaagagt gcccacgtga tgtcaaagat tcaagagtgc ctactatagt ttttgcacaa 11160
tcctgcacat ataatagcag caagcatatc acaacacaag tttgcgcatt gacctgcttt 11220
acacaatttg tatttacaca ctgcagcagc aattgtatgt ttcttggaca ttgatcatct 11280
agacaaacat ttgcaatatc agaacaaaaa gcagttcttt ccaaaaatgt tcggacattg 11340
tttgagtatt tccttgagaa ccattcttct tcctcttcac cgggatgcaa gtgaccaaaa 11400
atttatgaga aatgatggca tagcacagaa ccagctaaga aaagccatgg cagtagctgt 11460
ctcaaactct atgcagtgat tctgagagca gacaccaagg tcattgccaa tcagtacagt 11520
aatgccagca gaagcacagg ctgcagcaaa cgtgagggta gaagtgacct gcaagaaagc 11580
tgatattttt acaaggagat gaatggaaag gagtcgtgaa gctattcaga tgcttcagga 11640
tgagcttata tacaccttgc gttaaatgca caaagttgaa tgtggcgcac acatattccg 11700
aaaaagttta taataatgga gtaaaaggaa gagacaaaat caagaagtgc aaaatggaga 11760
taggtacatg ggatttgctg ttataataaa caccaactct ggttatcaag aaataataaa 11820
ctcttaactc cagattatga gatatttaaa agatgataga gcttttttaa ctttgctaca 11880
aggtaatttc cacatcaaat tataagggtt gtggagacca ggttaaaaac accaatttca 11940
tgtctgacct cacagacctt gagggatcag cgatggatca gaaatgccca agaatacatc 12000
aaaaagtagc aaaatctcct acaaaatgaa cagaatgagg atgaaagttc ccgggtaacc 12060
gagctaaaag ggctcaaaaa actgtttcag ttattgcact gaagttgtcc aagctgtgat 12120
taagtcttac ataagctgtg tcactctctt tagggacaag tgatttactt ctggatccct 12180
agccatttct atcataactc tttcatgcaa gagtttatca ctctattcat aaataatttt 12240
gcagtcaatt ttattccgaa tagaaacata gcgagactat atttacatta gccaatttaa 12300
agtcccaaat gagccatgac atatatcaat aagcatatta aacttggaga gagaacgaaa 12360
gagggcctgc tgccaaagtt tacttgcaaa gaggaaattt tagaggaaaa gtcatattta 12420
ctaattctct aataccattt aaattcactt ttaaacaatt accatgagta gcccaaatca 12480
ttgattacgt ttaactgcgc atcttccata tctttttgag ctccttaatt ggctattgcc 12540
tattgaaaaa ttcatatacc acaagtatac gaccaacttt actgttgcag ttaaatactt 12600
gcttatgcag ctctccagga tatgcatcct agattctaat ttatctatat ctcccaaatg 12660
gaaaggcaac caagagaatg tgaccatctt aaacgtaaag ggtaactaat tttaatattt 12720
ccttgctgag tttacagtaa aaaagttagg caactgaact tctctatttt ttttttctgt 12780
aaatactcta tggatagacc atcttactcc cttcttccac tatccttttt agtgcacccc 12840
caaaagattg aaacatttct atttacaaaa actacctccc aacactatcc ttttccttaa 12900
ttaatgctca tttctgccaa gagtgagcca tcagcattca tgatgaagta tatcatgata 12960
gaaagacaat gctggcaact tgcatttgtt ttacttatgc gtctatacac aggggcggat 13020
gtagttcatt accgacgggt tcaattgaac ccataacttt tgacgcagag taaaaatcat 13080
aactttaaaa atataatagg ttcaatgcta aaaactttaa aagttgaacc cataggattt 13140
aaatcctgga tccgcctctg tctatacatt tcaaaaattc actttccaat aaaactacct 13200
cataatatgg aagtgaccga gtactaaagg tcataagttt atatgaattc aagttcatga 13260
aatactctta acagctagcc aagttagcaa aggctatgcc ctacttcttg agttgtcacc 13320
cacgttttat gagtttctca tagttgcatc atcacagtgc ctagagggga tatgtaccaa 13380
cttaatctta ccaattagtc taaagaaaat aatttgacat gtatttgtgc aagacgttga 13440
agcaagctga tagaggttgt ataatggcac aaaaaccaat gaaggccatt aattttttaa 13500
tccaatatat atttttagaa tccctaaaaa aaatgcttgg aatggcattt cttaaagtat 13560
ttagtaagca tacaatgagg tttctaagaa attaatcatc tctctcatgg cattatatat 13620
tacacaacct tccctaaaat tttgatacct atacgagacc ctctgaatgg gtcaaaaact 13680
gaataactcg agaaacaaca aagaaatagg tagtactttt tcttatcgta ccttataaaa 13740
aagaaataat taattttcaa actttatgcc ataaaggatg ccctatccat cactggttta 13800
ctcatgagac caagcaagtg gaataatcca aagaaagagg ggtgagggag actcctaatt 13860
ttttgctcca tttagatttg taaatgcatt ttaattccct catcctaatt gtttgcttca 13920
ttacgatata gttaataaac gcattttaat aaaattagta gaaatgcata tttccaggtg 13980
attgttttgt attaaccaaa cataataaat aaggatatta acaaaatctc tagtacatat 14040
tgcaagatcc attaaattgg atggaggaag tatgccgatc acttcacaaa ctattgacag 14100
tcttttacta tttggaagga ccaaatttgc acatagtgta caaagttttg tgattaaatg 14160
cattaaattg gtataatata tttggatctt aagtcattca attcactgca aaactcgtga 14220
aattgtgacc ttggacctta aataggaaca gaaggacgaa aggccagcgg aaattctgtt 14280
aaatacatgg tcattatgga caagagaaaa taggtatggt cattgcacag aattactcca 14340
tgagtagatg tgaatattga cagttaatat gcaaggaatt gtgttaaatc cagcacaagc 14400
ttccccactt ttgagctagt caattttccg gcacttcttc catactagtg gaggtaacgc 14460
ccgtg 14465
<210> 56
<211> 9831
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 56
acaagtgttt attgcccaga caggagagaa gtcacattgc caattacatg gatctagttc 60
ttatagagtg cctggagaga ggaaagcaaa tcaattgtct gccttcatta tcaagctgct 120
cgacagggtt ataaatggct ccaaggctca tgctactctt tatggcttta ttctaaccac 180
agttctggat agtctcaatg tgcctctaaa gaaatgggaa atgatctcga gaaaggatca 240
ctttggcatc aatactcttc ttgcttgtga ctatgcagtc aatgacatcc caaatgaacc 300
tggtacatcc tagaagacac ccatcaacag caaagtcagg actctggttc aggaatatgt 360
agccaaggat gctgaaatag ctaggctttt ggctcgtgtg attgaagtga aatttgagag 420
ggatggtctc agaactgagc ttgacaaaga aaaggagaaa aatgatggaa ttcttcataa 480
catgctgaac cttctccaag cccaaaccca accatatagt tcttccaagc cttaggactc 540
ctagcttttg tctcctgaac ttgtttagta cctcagtgac ccagattagg gatttttcta 600
tcttttattt ttgctcatga tttggatgtt tttctttctt tttgtggatt gttggtggca 660
acatatctct gtcaatgata actactattt tgctcttgtt taatgttaat ttgtccttga 720
tattttaaat atattttctt gattactgat gattactcca tgattacatt tgcagttgcc 780
gcagtggcca tgggtactta ttaaaatctg ggaatcacac tttgtatgta acatttcgat 840
gatgccaaaa gggagaagag agttgtgctt tacacacatt ctgaaataag taatatttat 900
aacctaatta acctggtcct tgatgttaag tgaattttct aagtttagta ttgatggtta 960
agctgagttc ttacaggtcc caaataagta aaaagcacag agtttgtcat catcaaaaag 1020
ggatatttgt tggcccaaga acaggtgaag ttttgaagat tgacaaaaga actcagacat 1080
ggaccaggtc catcttgtga agcacagtca tgatcaacct atacatgtga gatgcacgtg 1140
aaagagataa gtcttactga ttaagcaaca atatctcttg atctgatcga aaaggatgaa 1200
gatagtgtta gagtttgaga tcatcatgaa ctcttccacg atagaagagc agcaattgag 1260
tcacaatcaa actctgatta ctaacctatt aaatgtcagt ttgttctctt ttacaggaaa 1320
tacacatacg caaaagttaa actaaattga gagcaaaaga gcaaggcgat tttgcaagca 1380
atttatgtgt gatttgagtg tgcactcctg aagctacttg aacgagatag aagaaccagt 1440
tccatcgtgt ctatctttta ttctagttca attgtagtag gtggtttaaa attatacctt 1500
tcagctttca tagaagcaat tgtattagat acctagagtg ttcaagttat agctaacttg 1560
aagttgtcgc aacagttgag gttgtgtgcc acaacgggat tagagttaat ccttaggttt 1620
ataaagagtt tttgtaaaag ctattttggc tcagtgattt tagtggaagt ttgggaaaat 1680
cctactgagt tgtaggtcat ggttttttca ccttttgagc caggtgtttt ccacgtaaaa 1740
atctccgtgt tctttatttt ctgtatttat tattccgcaa ttagtagtag ttggaacacc 1800
tagaaaacca agttcttcta tagagtagtt aagcgaaaat tgggtgccac acaaatcacc 1860
cctctagtgt ggtattgacg cttaaaacat caattagtta atttctggag caactagcta 1920
ctagttgtta ttaaaatagt tagtttcttt gttagctaat atgttgtttt ggttgtaaat 1980
tagttccggt gtgtagtttg gactttggaa gaagacttgt accagattgt agtttgttat 2040
tttctttggt cttatgaatg ctcacactaa acatcaagtt ggtactttga ttttgcattt 2100
gaattagaag tagtagttga gacgtgttgt tgctatgcat aagtagtaaa agattggttt 2160
gagcttagtt ggtttcgtga taagattgga aataaaagaa atgtgtcaaa gttatgtaaa 2220
aatcagtaaa ataggctgct accttttaat attaccacaa gtccacattt attttagttt 2280
taaacaatat aaatttgtta aggtaatctt cttacaatag tctcatcttt taatttagta 2340
acagataatt gcaaagtcaa tccaactaat catacactac ccatatgtaa aaaaaaaaaa 2400
aaaaaaaaaa aaaaaaaaaa aaaaaaagtg aacaatcaat gcacaaaaag aaaaaaaaat 2460
atttttcttc tttaattaat tccataacat agtccttaaa attagttaat tctttgtttt 2520
agaaaaattg taacagtcta gttaattctc caaaatgaag caaaagattt tttttcttaa 2580
gtattacgtc acttttttta ttaatccacc aaataaatta aattagattt agttcactaa 2640
ataaactcaa taagatcaga tgttttattt atttataaaa ataactcaat tacttaatca 2700
caaatgatca tgactaactc aaaagtaatt tgttttaaca aaaataatta atttcgtctt 2760
aaccgatgtc gggacgactc attttggaat aaatacataa ataaaatggc caggtcgcga 2820
ggacacgtca tattccaatt ctttcaaata agcttgttat ttattaactt tgagtaggct 2880
ccaattttag gtgcggcgca cgaactaagg tcggagatat tcatcttggt tagcgtaagc 2940
tagggttggg gatattcgtc ctagtttgag attaattaag tcatcaacag taaaagtgga 3000
cataggcaaa acatgaaaac cgaataaagc acaatttatc cataattaat tcatgccaaa 3060
tttaagttaa taaagcaact gtgctagaac cacggactcg gagaatgctt tacaccttct 3120
ccccgatcaa caaaaatctt tattcggact ttatttttgc agaccgataa taatagagtc 3180
aaatcttcct ttgactaggg attcaaataa aaagtgactt ggaacatgca aaaatcaatt 3240
ccaagcgggc gaatctgtaa acaaaataat ccttattcaa atttgtcact ttaattgaaa 3300
aactctttaa cccactattc ataacatata tatttttggg gtagaaaagg ggtgtgacag 3360
ttatgaccta ctttatgcat cagtgttcga atttattttg atcaacaccc ttttggaaga 3420
gcgtttgata gaaatggttg gcttaataaa caatcatatt atcatcacct gcggaatcat 3480
atcattaact tttgaaaatt aaaatggttt tcaaagacgt tttgataaaa gaattcctat 3540
tgtcgcagtt ggaatctaca agaccaagat gttgatctag tgctatattt ggagaaagtg 3600
ccttaattaa aaaaaaattg ttcattagtt gtcttaagat tttttattat ttaaaaaaaa 3660
attaagacac aaagaaacac atttacgagt atatgtcggc cgactaatgt gaagttcccc 3720
acggacaacc cacacatatt gtggtcaaga tggattctat cataatcaaa agtcatcatc 3780
aattcaattc tcatatttgg catctcaagt acatgcacaa aagcaactta ggatgtaagt 3840
ttatatgcac attcttgaaa tagaacctat ttagtacgta gtacttaatt agttacagta 3900
gtattattta ttctctgcta cagagctatg gtttatcaaa tatatcagat tatcatttgt 3960
tgtgtaggcc atttccttat ttgtacttgg tattaattct ggcaaaagca caaaactggg 4020
aaatgaggtt cttcttcctt aatattgagt cacagattag taccactact atagccaaga 4080
aaatgtgaaa tcatatagta ctaaatatta atttcagatg ccaaaaccat aaatttcccc 4140
tcctccatca ttgaaaaccc cctctgtcct ttcccctaga gagacccctt tttcctctct 4200
ctctcctttc tctttttatt agacgcatat attctctctt ctttctcttt ctagggtttt 4260
cacctgaaat agttttattt cggtgatatg ttaggatcct ttggttcatc atctcaatct 4320
catgatgaag aaactgatga tcaacggcgg agattcagtt ccacttcccc tgcaatccaa 4380
atccggcaac tactcattag ctgcgcggag ttaatctcgc ggtccgattt ctcggccgca 4440
aacagactcc tcaccatttt atcaactaac tcttcccctt ttggtgattc aactgaaaga 4500
ttagtccatc agttcactcg cgcactttct cttcgcctca accgttatat ctcttcagcc 4560
actaatttct tgacaccatc taatgttgtt gaaagttcaa atgattcagc tctacttcag 4620
tcatcctatc tttccctaaa ccaagtgact cctttcatta gatttagtca gctaactgct 4680
aatcaagcga ttttggaagc tattaacgat aaccaacaag cgatccacat cgttgatttt 4740
gatattaatc acggtgttca atggccaccg ttaatgcaag cactagctga tcgttaccct 4800
cctccaactc ttcggattac cggtactgga aatgaccttg atacccttcg tagaaccgga 4860
gatcgtttag ctaaatttgc tcactcttta ggccttagat ttcagtttca ccctcttttg 4920
attaccaata ataatgacaa tgatcatgac ccttcaataa tttcttctat tgttcttctc 4980
cctgatgaga cattagctat caactgtgta ttttatcttc acaggctctt gaaagaccgc 5040
gaaaagttaa ggattttttt gcataggatt aaatccatga accctaaagt tgtaacgctg 5100
gccgagagag aagcaaatca taatcaccca ctttttttgc aaagatttgt ggaggctttg 5160
gattattatg cagctgtgtt tgattcattg gaagcaactt tgccaccgag cagtagagag 5220
aggatgacag tggaacaagt ttggttcggg agagaaataa ttgatatagt agcagcagaa 5280
ggagataaga gaagagaaag acacgagaga ttcagatcat gggaagtaat gttgaggagc 5340
tgtggattta gcaatgttgc tttaagccct tttgcactct cacaagctaa acttctcttg 5400
agacttcatt acccatctga aggataccag cttagtgttt cgagtacgag taattctttc 5460
ttcttgggtt ggcaaaatca accccttttt tccatatctt cttggcgtta aatttaaaac 5520
cctaaaaaac aagattttta tctatctgca tggtgaagga caaagaggtc ttcaatctca 5580
ggttcttttt tttttttttt ttttatatat atatcttgtt tgggtttaag gttattgggc 5640
tgatgaatgt tttaatttta acataggtct acttacgtag tagttatagg ttgataatga 5700
gatataatta actaagtctt tgtataatgc agatcctgaa cttaatcttt atttgtatta 5760
tttttttttg ttactgaaag attctgttac caaattttat cagtctattt aattagaggc 5820
caacgattgt taggtatgtg gcacttcgag tgggaaatga tatattccca ttaaaggtgt 5880
taattaacca ccaaattgtt ctttaggtct gtttgtcatt ttgtattaag gtggatggtt 5940
tattatatat atcttctctt taatgctaat catgcttaac tttttcattt agtaccagca 6000
agcatatttg tttactttat tggttattcc ttatcaaagt cttcatcttg ttgctttttt 6060
ttattgtact ttacaaaaga tttctagtat taatggaaag tgctcatatt tggaaaaaga 6120
catggccaac aagaaatgtc tatatacccc atttcttctt cttcttcttt ttttccgaaa 6180
atttcttatt tttgttttta tttctgtttc ttgttgagtg ctttcatggt agaagaagaa 6240
gtaggagatt cttggacatg gctgcatgag aattgttaaa taaccccgta tacatacaca 6300
agtagtgttg gctgtctttg atatcaaacc atttattgcc ctaatttctg ccttttgtcc 6360
cctcaacaaa accatcaaag ttctcaaaga gggtttattc ttgtttccca ctttgccccc 6420
cacctattag ggccacccca ccaaagggat ctctctcgtg tctagtgttt tttcccaagg 6480
accaccactc cttttttttt ctctaccata acttcgtcca caccatctta ttgtgatatt 6540
ttcgtttaat gaatttgcag ccatgccttc attcatcatc agaactcagt cataagcaca 6600
gattctgaga gagtaattaa tgaatgaatc agtggtgatt tgacgtaaag tatacatgat 6660
tatggttttt agctgaataa gcagagggag aaaatatata catatataaa caagtagagt 6720
aaaagaatga cgcaagatta gtaccaaaag agtgaaggaa gagatttaat attataggga 6780
aaagggaagt agtaggtgat acttgacagg ttgataagat ggttattact acaagttgat 6840
gtattgacgc taactcacgc aagagagacc tactcactgt acaatatttt tacaagaata 6900
agcgattctt tctctcttta cttgcaagaa ttgtgtgttg tgtgagttgt atggcgcatt 6960
ttctaggagc ctgtggtagt gatggatgta ttcatataat acaatacaat acatatggaa 7020
tagatagata gataagatgg tgcacgcatg agaggcaatt atgcaactta cgtcaactac 7080
ttccatccat ccatcttttc ttccttctgt ttctgtctga tatagtgagt atatgcttgt 7140
gctggtgttg tgtgcttttc tggcctggga ttttcctaac actttagata atttaggttc 7200
ccatcaataa taatgtcttt ttagaggagc atcatcgata gatattcaaa tattaaacct 7260
ggcctagcta ctatctaggg cgtctgctag gtttttccat taccctttgt atatctctta 7320
tgtgggacct tttgtttatg gaagaatatg gagtactttt attcatctcg tagggtcttg 7380
aatacaagat tttatatata tcactcttta aaaatgacca tcctaaaatt cttcctcttt 7440
catttgcatt taccagaatt gatattagta cctaaactag tactcttcac tgaggccttt 7500
tgtatttagt cctattatat ttgaatttgg cactatttaa attaaaaaaa taatctacaa 7560
taaaaaattc ttccctaaac attacccatc aaatactcac cacctaaggt aactctacca 7620
tgtattaatt ttttggatca aatctagtga ggattaattc tccacttatg ttctttcgga 7680
actggctaag taatcttcaa aagctagggc atctccgcag tcatatcgtg ccctcccaag 7740
tatagcgacc gcttctatat tttccctgaa tttcatctgt gctagggctt gttttcacgt 7800
tgatttcaaa caaaggctaa caatttcatt gagtaacttt ttctcatttc aggactcgaa 7860
ccttaaacct ctgttcaaat aacttctaag aagtatatat gtatacaatg tttgtattca 7920
ttgtgacaaa gtattatgag ttgtacaact ttcttgtgaa gatagagcat aatgttaaac 7980
aaggatctat atagagcata atgtcaacaa aacaacaaca tcaacccagt aatcatccta 8040
ctaataggat ttggggaggg tagagtgtac gtaaacctta cccatacagg ggagggggta 8100
aagaggttat tttcgggaga ccctcggctc agagacaaaa aaatctataa taacaacaga 8160
aaccagacaa ataatatcag catcataaga gacaacaaat aagtggaatg acaataatta 8220
tgccaataat aacattgaaa aaaataaaat taaaaattaa aaataaaaat agtgtgatga 8280
acaaaaatcg ctagcagtct tagacaaaac actatcagac tagctggaac aacgaggaaa 8340
aacgctgaag taccccctaa cctacaaccc taatgctcga catccacacc tccctatcca 8400
gggtcatgtc ctcggaaatc tcaaatcgcg tcatgtcctg cctgatcacc tcgccccatt 8460
acttcttagg tcgccctcta cctcttctca tacctgtcaa agccaaccgt cacacctcct 8520
aactggggca tctaggcttc tcggggccgg ccagcccgta gatctaaaag gatccatcca 8580
tagcgcccga accatccacg cctcgctttc cgcatcttgt cgtccatggg agccacgccc 8640
accttatcca gccttatcca gcctagtgtg cccccacatc atctcaacat cctcatttcg 8700
actactttca tcttctggat acaagaattc ttgactggcc aacattcagc tccatacaac 8760
atggtcgatc taaccaccac tctataaaac ttgcctttaa atttcagtgg catgttagta 8820
tgtttacttt agatacaatg ttttttagag tcttatagtc ttgttagaat actatatatt 8880
ataaaatatg gagacttctg ggcacttttg ttttatttta tataagatag gattggaatg 8940
aattcaattg gagggacatg catgataaat gaatattcat gtagccgata tatgtttggg 9000
actgaaacga cattattatt gtgaaatatt ttacaattgc atttcacact cactgaagtg 9060
aaactttgat tccacgtcgg tcaatactta ggtgttacgg tttggctgcg aggggaatcg 9120
aagagagcaa attaattaaa gtatttaatg aggaatcatg agttagttgg tggaattata 9180
atagtcaaat gaatgagtta ttcgcctgat aatatagttg atagtagtat atactatata 9240
tgttgatact agttattggt ggtgacctaa ttaagtaaag agaagagaag agtggttatg 9300
taaaggaatc taggtatagt gggggatggg gggaggcaag gttaaaagaa aggtggaaaa 9360
tccaagaatc ctgcttcctc tagtaacata gcatatcctg caattcgtgc ttttgtttcc 9420
tctcacaaga taactacttt tttgattaat tattacattt gacacataca tacaaaccta 9480
taaaattaga catccttatg gaatcttacg actccgaact tgtcatatat cctttaatta 9540
tgcttagctt tttgctaaaa acaaaaagga tatccttatt ccaaaatgca actaggagca 9600
tcttcccaca tttctttttt atgcctctgc atcatcaaat cccataatgc cgcacaacaa 9660
tttcttttta cttgagtata ttctagctta gctatttcat acgaataatg ggtatacaaa 9720
tttgcttatt ttaggtttta aataccgatt taaatatatt ggatgggttc aacttttaaa 9780
attcttacac tgatatacat gcatagaata tgtggaaaac tttaatatta a 9831
<210> 57
<211> 10589
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 57
gacgtcttac ccggttcaat gcctattgca cctcgttgaa gaggctctac gtgcctactt 60
cattcatctt tgcctaatcc ttatcggtca ccaggcaacg aaggtagctg gctatgccga 120
ccggagcgga cagggctcgg gcgtccatcg ggatagtaag aatgaccatt ctcttacggt 180
cggggtcaac acccgggggg agggaacttc ttcactagtt tcgggctcaa gctcgagctc 240
ggcttgctcg ctcccgatga cacatccttt tttgggacct ccaggtgacc aagcctggag 300
aagtcctcca atgcgaccat gtccatgcca tcaaagaacg tgttgagggc attatctgga 360
cccgacgcta tctcatttaa tttctcttta ttagcttgaa cctcttcgag catggactca 420
gtataggacg gcgtctctgc gatgtcaatg atactagcta catccttcgg gacaggagcg 480
gcttctcgat aggccatgga ctccgtctct ccctctggtt ctcgagctag agggggatcg 540
acctcttggt cacctttcct ggttgccacc ttctccctct cgttgagggt cgactcatga 600
gcaatgaact caaggtcgtc atcctcagga taatccctaa gccggtagag ggaatcagat 660
ttcggtaccc tcgacttggt gctctttttg tttttctgga cctggacgac caccctcttc 720
ttcttcacct cgacatccgg ggagcctgtg gatttccttt tattcttctt tttctcttcc 780
cttacggcgg cctcgggctg tcccgaagcg gagggttcag cgagcgagga cggatcctcg 840
tcctcatcca atggcttgag ctcggtggtc atgggtgaac ctgtgagaag aaagaagact 900
tagcgatata ttcatcaagt atatgaatgt aatcattcgg gagagagact cactatggga 960
acgagcctcc catttgccct tcgagagctt gcgccatacg ctcgaagtag gacatctatt 1020
tgcacatccc ctcgattcac tccttgaagc aggggaatgt attaggaacc tgggcaactg 1080
ctacataatg gcaaatacag gcaattagaa aaaagaataa aaggaaatgc cagatactcg 1140
agagggaaaa gacttacggg atgcgtttca ctttttgagg aatggtagaa attcggagag 1200
gatgaggtct tcggttttca cccgaacgaa cctcccctac caacctcgat ctcggtcctc 1260
gtcgatgctc gagaacggag ccttgcttgc tcggcgaacg atcttaatca acccctctcg 1320
gaagattcga ggactgtata gatgaagtag atgttcgagg gtgaactgag gtgcttcagt 1380
tttgtttaca aagtgttgga ggaggattac gatcctccaa aaggacaggt gaaggcagac 1440
cttgcacctt ttatagatgt cgaggactat ggggtccacc gggacgagcg tgaaggagta 1500
agtgtaaaca cttaggtacc cctccacgtg agtggtgatg tctttgttgg gcccggggac 1560
gaccacctcc tttccctccc agttacactc tacccgaact atgggtagtg tctcctcagt 1620
aacagagcat atgtacctcc atgctccctc gtctcggtcc tgttgactgg aggctttctc 1680
gacgttgaag tcattctcga tagagcagcc cccgagtacg aagctcttca agggaggctc 1740
atgggctacc tcaggaatgg ccacctcggc atttatggcc agattggaaa ataaaggagc 1800
ggtttgttga ggaacgaatt tgaaagtttt tgctatcggg ttctgaaaaa tatgaaggtt 1860
tgaagaaaaa atatgaagat ttgaagatag aatggaaata tgaaggtctg ggttgaagat 1920
tgaaagagaa tgtatgaaga ttgaggatga aggtatgaaa atctaaggag caatctatga 1980
agatttgaag gagttaaagg tatgtaaaga attcaagggt aaatcaagga gctctagaat 2040
cgaaaagtgg agaagtgaaa aaggggtcgg agcttttata gaggaaggac aatcaatgca 2100
tgacgtttca cattcgagga cagtcaatca acggccgata cgtgtccgat gttagaacga 2160
tgcgactaat gggacgtttc attgatccgt catctcggtt gtaacgtacg aagaaaggaa 2220
tcggggttca tttatcgctt cccgtcgttt cgataaatct atcctccgaa aaacaagggg 2280
actatctgta tacgggtaaa atcaggcaat gtctaccctg attctcctat aagagaataa 2340
agggggagcg cggatccgcg agattgtaat cgaggacaga gacccatcgt atcaagatcc 2400
aagaagagtg aacgatatat ctaacatcag acacggcaaa gcgatgtacc ccggaccgaa 2460
tataactcct agacctcggg agaagcgggg gacggttatg catgacagat aggagactgt 2520
atactcgccc tcaatcggat attacgacgc gaatctcgtc agtaacaatt atggatcaat 2580
aattactgga aaaagaagat ttttaccttt tttagactta tactaggact gaaattctcg 2640
tactatataa aggtaaagtt tttctttgat ctgacacatt gtaacacgca attcaaagaa 2700
ataaaaattt gtttttgcct tctaactaat gttaaaaatt ttgctcactt gttctgttct 2760
tcattcacga ctggactcga accgagggtc caatcgagta cgaggtcact gttcaatcta 2820
agatcatgct tggtcataac attgcgattg gtttgatcat ttatttcgtc tttaattcat 2880
ttatctgtta tttttaatta ttcgtgttga attaaatcac gtatcattta aaccgcgtac 2940
aaatttaatt gttacccatt tttaaggtaa acaactatag acgaaaaaaa aaatataaat 3000
attaaatatt atgtttcgaa agatacacaa tagacaagaa aagaaaagaa aaatccctta 3060
taaaatttgg atttagccca ccagttttat tgagacgtct ttgtgtgtta gttacccggc 3120
aaaggttatg aacctacttt atgcgtcaat gtccgaattt atttttatca acatcctttt 3180
ggaagagctt ttgatagaaa tggttggctt aattagcaat catattatca tcacctgcgc 3240
tttggtgtta tatcattcgg aatcatatca ttaccttttg aaatttaaaa tggttttcaa 3300
agacgtttcg ataaaaaaat tcctattgtc gcagttggaa tctacaagac gaagatgttg 3360
atctagtgct atatttggag aaagtgcctt aattaaaaat aaaaaattgt tgatcagttg 3420
tcttaagatt ttttattatt aaaaaaaaaa aattaagata caaagaaaca catttacgag 3480
tatatgtcgg ccgactaatt aatgtgaagt tccacacggt caacccacac atattgtggt 3540
caagatagat tctatcataa tcaaaagtca ttatcgactc aattttcata tttggcatct 3600
taagtacatg cacaaaagct acttaggatg taagtttata atcattcatt cttgaaatag 3660
aacctattta atagtactta attagttaca gtagtataat ttattctctg ctaaagagct 3720
attgttcatc aaatatatca gattatcctt tgtggtgtag accatttcct tatttgtact 3780
tagtattaat tctggcaaaa gcacaaaact gggaaatgag gttcttcttc attaatgttg 3840
agtcaagatt agtactacta ctatagccaa gaaaatgtga aatcatatag tactaacttt 3900
cccttctccc tagctactga taactctaat taatttcaga tgccaaaacc ataaatttcc 3960
cctcctccat cattgaaaac ccctttgtcc tttcccccca gacccccttt tcctctctct 4020
ctctctcctt tctcttttta ttagacgcat attctctctt ctttctcttt ctagggtttt 4080
cacctgaaat agttttattt cgttgatatg ttaggatcct ttggttcatc atctcaatct 4140
catgatgaag aagctgatga tcaacggcgg agatgcagtt ccacttcccc tgcaatccaa 4200
atccggcaac tactcattag ctgcgcggag ttaatctcac ggtccgattt ctcggcggca 4260
aacagactcc tcaccatttt atcaactaac tcttcccctt ttggtgattc aactgaaaga 4320
ttagtccatc agttcactcg cgcactttcc attcgcctca accgctatat ctcttcagcc 4380
actaatttct tgacacctaa tgcatcatct aatgttgttg aaagttcaaa tgattcagct 4440
ctacttcagt catcctatct ttccctaaac caagtgaccc cttttattag atttagtcag 4500
ctaactgcta atcaagcgat tttagaagct attaacgata accaacaagc gatccacatc 4560
gttgattttg atattaatca cggtgttcaa tggccaccgt taatgcaagc actagctgat 4620
cgttaccctc ctccaactct tcggattacc ggtactggaa atgacctcga tacccttcgt 4680
agaaccggag atcgtttagc taaatttgct cactctttag gccttagatt tcagtttcac 4740
cctcttttga tcaccaataa taatgacaat gatcatgacc cttcaatcat ttcttctatt 4800
gttcttctcc ctgatgagac attagcaatc aactgtgtat tttatcttca caggctctta 4860
aaagaccgcg aaatgttaag gatttttttg cataggatta aatccatgaa ccctaaagtt 4920
gtaacactgg ccgagagaga agcaaatcat aatcacccac tttttttgca aagatttgtg 4980
gaggctttgg attattatgc agctgtcttt gattcattgg aagcaacttt gccgccgagc 5040
agtagagaga ggatgacagt ggagcaagtt tggttcggaa gagaaattat agatatagta 5100
gcagcagaag gagataagag aagagaaaga cacgagagat tcagatcatg ggaagtaatg 5160
ttgaggagct gtggatttag caatgttgct ttaagtcctt ttgcactttc acaagctaaa 5220
cttctcttga gacttcatta cccttctgaa ggataccagc ttagtgtttc gagtacgagt 5280
aattctttct tcttgggttg gcaaaatcaa cccctttttt ccatatcttc ttggcgttaa 5340
attataaggg aaattaaaac cctaaaaaca agattttatc tatctgcatg gtgaaggaca 5400
aagaggtctt caatctcagg ttctttttgt ttttttaact tgtttggata tgaggttatt 5460
gagctgatga atgttttaat tttaacatag gcctacttac gtagtagtta taggttgata 5520
atgatatata tttaactaag tctttgtata atgcagatcc tgaacttaat ttttattttt 5580
attattttgt tgttaatgaa agattctgtt accaaatttt atcagtctat ttaattagag 5640
gccaaagatt gttaggtatg tggcacttgg agtgggaaat gatatattcc cattaaaggt 5700
gttaattaac caccaaattg ttctttaggt ctgtttgtca ttttgtatta aggtggatgg 5760
ttcattatct tctctttaat gctaatcatg cttcaccttt tcatttagta ctagcaagca 5820
tatttgttta ctttattggt tattccttat caaagtcttt atcttgttgc tttttttttt 5880
tattgtactt tacaaaagat ttctggtatt aatggaaagt gctcatattt ggaaaaagac 5940
atggccaaca agaaaggtgt ataccccatt tctttttctt tttctccaaa tttttttttt 6000
tttttttctg tttcttgttg agttctttca tggaagaaga agaagagtag gagattcttg 6060
gacatggctg catgagaatt gttattgttt tgtgcactta aataaccccg tatacataca 6120
caagtagtgt tggctgtctt tgatattgca ccatttattg ccctaatttc tgccttttgt 6180
cccctcaaca aaaccatcaa agttctcaaa tagggtttat tcttgtttcc cactttgccc 6240
cccacccatt agggccaccc caccaaaggg atctctctcg tgtctagtgt tttttcccaa 6300
ggaccaccac tacttttttt tttttctcta ccataacttc cacaccatct tgtgatcttt 6360
cgtttaataa tgattttgca gccatgcctt cgttcatcag aactcggtca taagcacaga 6420
ttctgagaga gtaattaatg aatgaatcag tggtgatttg acttatacat gattatggtt 6480
tttagctcaa taagcagagg gagaaaatat atataaacaa gtaaatctag tagaagaagt 6540
agaagtttta tagctagagt agtgggaaag aatgacgcaa gattagtacc aaaagagtga 6600
aggaagagct ttaatatagg gaaaagggaa gtagtaggtg atacttgaca ggttgataag 6660
atggttacta ctacaagttg atgtattgac gctaactcac gcaagagacc tactcactgt 6720
gcaatattta caagaagcga ttctttctct ctttacttgc aagagttgtg tgttccgagt 6780
tgtatggcgc atatgaacct tttttcatac aatacaatac atatggaata gatagataag 6840
acggtgcacg catgaggcaa ttatgcaact taacatcaac tacttccatc atcttttctt 6900
ccctctgttt ctgtttctgt ttctgtttct gtctgatata ctatatgctt ctctggcctg 6960
gattttccta actcttttga taatttaggt tcccatcaat aatgtctttt tagaggagca 7020
tcatatcgat agatattcaa atattaaacc tggcctaggg ctagggcgtc tgctaggttt 7080
ttgcattact ctttgtatat ctcatctgtg ggaccttttg tttatggaag aatatacttt 7140
tattcatctt gttgggtctt aaattcaaga tttaatatta ctctttaaaa attaatgact 7200
atcctaaaat tcttcctctt ttatttgcat ttacaagaat tgatattagt acctaaaact 7260
cttctctggg gccttttgta tttagtcctt ttatgtttga aattgacact atttaaataa 7320
aacataatct acaataagat gttcttcacc cttcgggttg cccggttggt ttggatggga 7380
tcgattcccc cgatatcttc tgggttgagc atatcgcaca gggcttgtct agtgcggttt 7440
gcattcccta tgtggtttgc attccctatg tggtttgcat gctattatac atgggtttac 7500
ccagtggaca caaagtattc aatacagagt gttcacccga agaacagagg ctgtggcaaa 7560
gattgtaacg gccgcgggtt tcccctctta caaaaaaaaa aaatgttctt ccttaaacat 7620
tacccatcaa agactcacca aagatagctc taccaagtat tatttttgga tcaaatggca 7680
tttccacggt catatctcct cccccccccc cctccccccc ccccccaaag ctagtgatca 7740
cttccatatt ttttcctgat ttcatcggtg ctcaaatact tgttcattca tcttcattcc 7800
aaacaaaggc gaaaaacttc actattgagt gcttttttcc tattccaagt gtcaaaccct 7860
aaacttctag tcaaataata tctaagatgt atactcttat actatgtttg tattcattat 7920
gacaaagtat gatgagttgt acaattttct tgcggactta gtgaaaatag agcataatgt 7980
taaaaaaata tttacatgat attaattagc cggattaagt ttataacgtt agtatatatg 8040
tctactttag gtacaataca agtcttatac tcttgtcaga atttatatgt cacaaaatat 8100
agaaacttct agctactttt ttttaatttt atataatata atattggaat gaatttaagt 8160
ggagcaaaag tgaatattca tgtagtcgat atattctaat ctgtttgggg ctgagatgac 8220
atgattgtag tgaaatattg taccattgca tttcacactc actgaactga aactttggtt 8280
ccacgtcggt gatcatttgc atgtttcatt agtcaatact gtggctgtta tgatttggct 8340
gcgaggggga tcgaagagag cacattaaag tatttaatca ggatttatga gttgaatgct 8400
gttagttggc ggaattaata gtcaaataat gaatgagtta ctcgctgata tagttaatag 8460
tactccgtat atatgttgat tctagttatt ggtggtgacc taagtaaaga gaatagatga 8520
gaggagtggt ggtatgtaaa ggaatctagg taaaggggtg ggggtggggg gaggcaaagt 8580
tgaaaagaaa ggtggaaaat ccaagaatcc tgcttcctct agtaacatag catatcctgc 8640
tattcgtgct tttgtttcct ctcacaagat aactactttt tgattaatta ttacatttga 8700
cgcatacaaa cctataaaat taaactaatc aacgacatcc ttatggaatc ttacgagtcc 8760
gaacttgtca tatatataac tttaaagtac tttgtcactt cttaatatgc tcctttaatt 8820
gtgcttagct ttttgctaaa aaacaaaaag gatatcctta ttccaaaatg caactgggag 8880
catcttctca cttttctttt ttatgcctct gcatcatcaa atcccacaat gccgcacaac 8940
aaactcttgt tacttaagta tatattctac ttcataagaa taatgggtat aaaaatttgc 9000
ttattttatg ttttaaatac caccgaaaat tcataagcaa attcaggatt taaatatatt 9060
aaatgaattc aacttttaaa attgttgcac ttatatatat atatatatat atatatgcat 9120
atccaagttg agggatacgg gttcacatga actcatatta ctttctctaa accatgtata 9180
acaatgttat attttttcaa aattatttaa atatatgtgt gtgaacccat tctcaaaatc 9240
tcttatggtg caattattat tgggtgcaca tctacaagtg aaatttgcag ctcaaaacct 9300
catctgggcg gtcttgtttt ccgcatggag tataactata tatgtgaaaa ttactagaat 9360
ttcaaaatga atataatttt gaaatgttgt gggttcctgg taagagacta aagttaaact 9420
cgtcaaatat aaattctaga tccacctctt cacaatagtg cacccattct tttgaaattc 9480
tggatctgcc tctgttaata atatatatat atatatatat atatatatat atatatgaac 9540
acaaaataat atgtggaaaa ctttactatt aataccactg ctaaacattt gaatggattc 9600
ttcatgccgt gtgctccttt gttgaagaac acgtacttgg gagggcgaga tttcgaataa 9660
aaaagttata ctaataacaa acagcaacaa ttataagaaa atgaaaataa aagggaaaga 9720
gcactcacat aaactagaaa ctgtagagtt ggcaagtacc aggtatatat gtccttgaat 9780
gttttttacg aggaattgag taaaacgcta gctatttcaa cacatatata aaaagcatca 9840
ataccaattt tatggtttct cttaggtgtt gatagattct cttttgtcag caaagttctt 9900
gcattaacta tatgaaattt ataataaaaa tgctgctctt ttaattgagt atacatgcag 9960
tctcctaaca tatacattct ccgtcccgat atatacttga tttgatgcat ttcaaaaatt 10020
aaatgtttga gtgttttggt gaattgtgct tgatatagaa gtatttaaaa taagaaagaa 10080
atgtaacggc agaatcttaa gtcgaaagtc aaattaaatt tgaaaaataa aaaataatac 10140
tcttgatact tactagtact agtcaatggg cagctctttc gggactaacc aaaagcatta 10200
ttcttattgt ttccggcata gtattaaaat gtaacaatgc ttaattatgt tacaaaatta 10260
atgtttttgt ggacttcgga ataatttatt tctgaattcg ccggtgttat cgaaaacatg 10320
gggacagtcc ccccaaaatc cacgggttat tgtgcaaaga cgaacataca agtttatttt 10380
tgataattta atcccaagca tcaactttct catacttctc taatcccttt gcatccaaaa 10440
ttatcaaaaa tagagaatat taaatgatag ggggaaaatg atcattaaat gcttcccttc 10500
cgggacatcc ggtttaaggg gggaaaagga gatattagat gcatgcaaaa taataaattc 10560
ttggaagtaa ttaatccgaa gttctgacg 10589
<210> 58
<211> 9770
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 58
gtctacaaga aaatatgcat ccggatattc aaaaatccaa aagaaaagaa aagttgaaaa 60
tttaataaaa tctcaaaaag gagttctcga gaattttttg acaaataata aaaaaactaa 120
atcgcaaaat gtaggagaat attctgtaga tgaacaagtc actaactttg agtcagatga 180
taacaaaatt caaattgaag aagatgttta tgagaaatct gacgaagaaa caaactttag 240
ccttaggccc tatattaaac tttggcccta ggcctcatat gagcttgagt cgcccctaat 300
tctaatacaa gtattagtgg ctactttcac ggcttgtgac ttatggatta caaacaactt 360
tacagttacg tcaaggctcc acgtagttct caatttatgg agcatatatt agatgattaa 420
cgcagggaaa gattctgctc tcctctgata catggctatt attcctcgtt tagttcaaaa 480
aggaaaaaga gggtagtctt gttatattat tggggaatga attatggttt caaacttttc 540
aaacttaaag gattttgtac atggtaaaac ctaaattgac acgtaacttg gtactttcaa 600
agacacgatc ttttacgcga tattttaaat aaagaaaaga tcaagtcaaa acatgggcca 660
aaaagaaaaa ccccatgatt ttttctgata aaaagctgct aacttttagt ttgttttatc 720
caataaaaca tctttaacgg tctgcctgct ttagtttaat cctcttttta agatgtaatt 780
aagcataaaa tagaaaaggg aaaaaaaagg tccattggat tttggaagaa attttaagaa 840
agtacaagaa ctagtaaagt cattttgtat agagtatgtt aaaaaggtga gtgacaattc 900
gaaaaagaaa gcattgataa gtcaatcact aaataaaaaa gcacacctaa taatcattca 960
ttcaaaaaaa caaatttcta tgaaagataa tcattatcat aagtcactgc agaaatccca 1020
tatacagtag agtaccagga ttttacgata aggtgttagc aaactatcta ttcatttttt 1080
gacaagcatt ttatgtttgg tcatttgttg ggaaaaatta gggagaaatt taaaaatagt 1140
tagatttaca actggtcatt aaaaatagcc caatttcaaa agtaatcgaa atttagccac 1200
ttttcatgta aagataaatc tgagcgaaaa tattgttcaa aacccggaaa atacgcccgt 1260
atattatact ggagttccag cataagtatg cttgaactcc agcatattat acgggagttc 1320
taggataact atgttggaac tccagcataa tatgttggag ttccagcata agtacactag 1380
aactccagca tattatacgg gagttccaac aagtataact gtcccgtata atatattgga 1440
gtttggagca ccggtgctcc agtctcccgt atattataca ggagtcagca aagtataccg 1500
gtccagcata atatgctgga gttcgtacac agatgcaccg aactcacgta tattatgcgg 1560
aaccggtctc tgttgcagca aaatagtggc tatttttcat tgacttcgta aacggtggct 1620
atttttgaat gaccagtccg aaaactggct ataccgtgct attttgacga aaaattatcc 1680
ccccacccac ccacccaccc aaacgcacct tacacacatt agtgcacatc ttttaactag 1740
tttttggtta tttttttatt tgatgcccga tattcgtata tggatttcga ttaattagaa 1800
ttcacaccga aacattcttt cttaggattt tgtacatact taatatgcga atacaaaacc 1860
tatgcggaaa ggtaagggaa cctattcatc cctctacagt acttgtgata atgttatact 1920
tttttgaatt taatttggga gacatgtcaa tctttatttt gaaaaaaaaa tagaataaaa 1980
ccatagggaa atgaacaatt tatctttcac tcctatctca ttttatttgt cttgaatttt 2040
tcaaaatttt gaattatatt ttgaaacttc ttcaatttat tttcttggaa tcttcagaat 2100
tcaatttaaa attccaaaat tccaaggatt tagctcccgt ttggccacag attttggctt 2160
cattttttta aaaaaaattt tgaaaacatt ctttgtttat gcaatatgat catgttttag 2220
gggaaaaaaa ttaaaaaaaa taaaaaaaaa tcaaattccc aaaaactggt taggcaattt 2280
ttggatgata ttttttcttc cactcacaaa actttaacat gtccaaacac aacttcaact 2340
tcaaaaatta ttttcaacac aattttaaaa actctttttt caagtttcaa tcaaatctat 2400
atccaaatgt tagcttagta tcaaataagt gattgaaatc aaattaaaat cgagtggtaa 2460
ataaaataga ggagagctcg gtaaattaca agagtgcggt aaatcttttc tcctttactc 2520
tcactgtagc ctattctatc tgttgtaact aataagtaac tgagctacgg aaaaagtgcc 2580
tagactttta acttcacaag tataataaat agaagtcaat tctttcataa tattgtttcc 2640
atcctatcaa acagactttg tctcactgac cttccttctg agtgtgtctt ttatatgtca 2700
tttttagtga atccatatga tttagagact ctaatattcc acatgcgggt cttaatttgg 2760
tgtatatgta tatggtaata atttttgtta ggtagctgta gtattctatt attgttatgt 2820
attgactcat catgtaaata aagccggtta gataaggcta gaaaaatatg agtataccta 2880
gaaattatta gcatattgtt tggaacatgt caaaaatttc aatgacctag ctagagctgt 2940
caattagtca aataacttta ttaatattta cttatgaaaa cactttgaaa ttcttggagt 3000
ttaagggaaa gactactgac taaaaaacaa agcaaaagtc tatgcattac tatactatac 3060
acagcacagc attttccaat agtatttgag atgaatctcc aatcagctac tgttgttctt 3120
ttcttttctt tatttagttt aagttttatg tgttgatggt atacaaatta tttgcacaat 3180
caaatggctt atctggataa tataggtaaa cctcttgtaa tcactaattg gtaatctggt 3240
aaaaataaca ctatttctat tccaatttat gtgatcaatt tcactagaca aaaatttaag 3300
aaagaaataa attttttaga acttgtagtc ataaacaagt tgtaacattt gtatggctat 3360
aattttttta acttgtgatg ttaaacatgt cagattgttt gtgtagctat aaaagttttt 3420
cattaggcgt aaaattaaaa atttagatta aattattatt aaatttagaa agaggtcatt 3480
ttttttagcg aagtaaaaaa gaaatcggtt cacataaacc gaaacataga gtaagtaatc 3540
tgttatgaca aattaaaaat tacttgtagt gtaaaaaaat ctttacaaca ttcgtgtata 3600
tacttaaatc ttttttattt tttggcaaga gatagttgtt cagcaaaagt aagttagaaa 3660
taggtctgtc cttctgactt tgtaactctg aaatgaaaat ttcaaaatcc cttctatttt 3720
tactgttacc cccccccccc cctcacaaac cccaactcac tcttatttaa taaaaagctc 3780
tacttagaaa agacaccctt gtccatctgt ctatataggt agaatgagag taaaggagaa 3840
aacatatcct cctctccatt tctgtagaca aagattctca aagagaaaca aattaaacac 3900
tagagagtga gagagtgcta taagaaaaag aatatgggga gagctccatg ttgtgataaa 3960
gcaaatgtga agagagggcc atggtctcct gaagaagatg ctaaactcaa agatttcatt 4020
cacaaatatg gaactggtgg aaattggatt gctcttcctc aaaaagctgg taacaacaac 4080
ttctactcca ctagtcctct atgtgtatgt attttattat tattattatt attattatta 4140
ttattattat tattattatt attcatgaat cgaagggaca aaggtctaaa tctcagtggg 4200
tcgtggtagc aaggccattc cgccatttat aatatcttct tgcaaattcc accagtttca 4260
tatgtgtatg tttttttctt attagtcata aatcaaagcg acgaagggtt aaatttcagt 4320
tgattgtgat agcaaggtca cactctaccg cttataatat ctcgtggcgt atttaacatt 4380
gtttgtatgt atatgtttga gtataaaggg aggaaagctt atatttatat ttgagtggat 4440
tgagtttttt tccttgttgc tgcattattt atgatttgat gagatttatg ttgggaactg 4500
caggactaaa gagatgtggg aagagttgta gattgagatg gctaaattat ttaaggccta 4560
acattaaaca tggtgatttt tctgaggaag aagatagagt tatttgcacc ttgtattcca 4620
ccattggaag caggtaatat atatatacct ttttttggtc gtaatttttt tttcattttt 4680
tatcatcttt ctgatgaatt tgagactgaa acaaaaactg ttcccactaa aaatggaaaa 4740
gtaaaacctc aataagtaag aaaagggaaa aaacaatgag ggctcagaaa gaaatgcaaa 4800
tagtcagttg gatttttaat taaagattct gccatttatg gacatatttt tctgcatgca 4860
tgccaggttt agatctaaga tcaagtcttt atttactcac ttacagatgt ttaattatta 4920
agacaaagtt ccaatttttc ttctttcttc tctttctttt tgtggaaatt ttttctctag 4980
taaaccaatt aatttttgtt ataacatgtg caatataata tgttaacagg tggtcaataa 5040
tagcagctca attaccggga agaactgaca atgatatcaa gaattactgg aatactaagc 5100
tcaagaaaaa acctatggga ttaatgcaat caactaacca aagaaaatca ccatattttc 5160
cagctactaa ttctcttcaa acccaacccc agataaattc aagtcttttt agagacttat 5220
attacacccc aaataatagg cctaatatta caggcctaaa tcatcagtcc atttcttctg 5280
cccaccagac aaattttctc tacactaata ataacatgaa ctttcctaat ttgggtgcta 5340
caaataatca atatccttat aatatccaaa gtcataattt acttatgttt ggagaagcaa 5400
gttgttcttc atcagatgga agttgcagcc aaatgagttt tggtaaagaa atcaagagag 5460
aagaaattat gagtaatagt ttacaacaag gtcaaatttc aagtgttaat gcttttgaag 5520
aaaaccacca gaattttact cttgattatg gcaatagtag tagtaattgg gtggatcaaa 5580
aaccaaatgt gtattttggt actactacta ctcaagtact tcagtatgat aatgttgaag 5640
aagttaagca gcagctaaca agttgtacca atggcaacaa tggtagtact attggatgta 5700
acaacaacaa cagtatgttc gtgttcaatg atgagaatta taacaagtca aatgagatag 5760
agatgttcta ttactaaaga agaaatgact gttgaaaaga aaacaaatgc aagtaccatt 5820
aggaagattt gaaagggcgt ttgggtatgg gggttgccaa gaagattcag actttttttg 5880
gggttttgtg tagttgtggt agaattatta ttgaatgaaa aaaaaaaact tcctgtactt 5940
taattcgtca gtacatacta catactacta caaagtagtt aaaagcctat tctatttgtg 6000
cttttttttt cactcgatgt tcaataatta tattggtttt tgattaaatt tgaatttgag 6060
caaggaagat caacattgga gggataaatt gtttcctaac gaaggcgatt acatacttag 6120
aacttgaact caatatctct aattaaaaat gaagtaatac ttataataac tccaccacaa 6180
ttcttattgt tgtgcatttc tttataaaat atgtaaataa tgggtcatat atattgttta 6240
ccttttctat tcatatacat agattttaaa ttaattatac acatatatat aatacattaa 6300
ttattcatat attatatttt tgctagctat ttttagttta agcgatttgg taggcgacta 6360
cttgggttaa ttcttttttt ttaatatata tatcaaaata atgaagctgt ataatacact 6420
taaaaatcat atttgaaagg tattaaatac gacttaggag agttcttaaa ccattttgga 6480
accttgtcta cgtactttta tgcaatagct gtttttgttt gtctctgcta aaacctatgc 6540
tccccaaccg tgcaccaatc aacttagaag ttagaactca gaaataaatg taactatact 6600
ccacagaaag ttaaaaagtt ttactgttac cattcactca aggatcagaa actgaaagac 6660
aaatgaatca gtgcttcact gttcttcact aaaagaaata ctgtttacat tagtttcaaa 6720
agagtttaat cataaaaaca aatgtaccat aaaaagggga gattatcaac ctgaaaatga 6780
aacagaacat acgttatata tcaatctata tacggtcgag atcggactcg tctattacac 6840
gacagatcgg gattgaaacg taacagtttt gaagatcaac cccgggttcc gtcggaccga 6900
ggtacaaggt cagaatgccc gttctcgaga acatcgagtc catgacccca gaatcaaccc 6960
tgaccccaaa tgagctcgag gaaacatccg gataacggaa ggcgaaatat ccgtaaccgg 7020
tcgggtatca cggcatgaat ttcggcacgt aacaatgaga aaccggctaa ttagcaaatc 7080
atggaatttt ttacctttta tagaattgta actaaagtgg gattccccta ctatgtaaag 7140
ggggtctgac tatttgtacg ggacattcat taaacgcatc ccaaagtaat ataatattat 7200
tttctttttg taagctattg ttctcctgta tctgatacta tttgaattgc atcaagttca 7260
agtgagactc attttttcaa ggctataatt gttcaagtcg cacggtttga atttattcga 7320
tcattgttcg ctttaattac aattcaattc atcgctttat gtcaaattaa tccacatatc 7380
cttaaaacca cttacaaatt taattgttat caaattttaa gggtaaacag tttggcgctc 7440
accgtggagc taaggataat agtggttgtt tgatatagat tttcataaca cacactattt 7500
tacaattgtt cttcgaagtg tctctcattt caggtttaag ctcaaaatgt caaactcaca 7560
attggcaccc ctacctgcac acaatgagtc tggtcaccat ggtgaaaata acaacatagc 7620
acctggtaac gaggtaccgc ccgctgatcc catcagaatt tcaatcgcgg acccgttgga 7680
cgctaactcg catgtggcta tcgacatgtt acagtctcaa caggcgacga tagctcagtt 7740
acaaaaccaa agccgcacac cgagcagagt tgaactcgat ccgtcccgga aaatcacctg 7800
cagggaagaa ccgtccgcgg agaggtcaaa tggagatgag tcggggacta accccgagat 7860
cataaaaatg cttgaggaac cgatgatacg gattgaatca ggggaaaaga aaatcgaggc 7920
aaatgacaag aaggtaaaaa cttacaattt cacggtcaac caaatcccgg gagcaccgcc 7980
ggtactgaaa agcttggatt ccaagaagtt cgtgcaaaaa catttccctc cgagtgtggc 8040
cccgaaatcg atcccaaaaa catttatatg cccgagattc ttaagtataa tgggacaacc 8100
gacccaaacg agtatgtcac ttcttacaca tgccctatca aagggaacaa cttagaggtt 8160
gatgagatcg agtctgtttt gttgaagaaa ttcggagaga ccctgtcaaa tggagctatg 8220
atatggtatc acttacctcc taattctatt gactcatttg caatgcttgc aaactctttc 8280
gtgaaagcac acgccagggc tatcaaggtc gagacccaga agtcggacct cttcaaagta 8340
agacagaagg ataatgagat gctcaaagag tccgtgtcct agtttcaaat gaaacagaag 8400
gacctaccac cggtcgctga tgattgggcc gttcaagctt tcacccaagg actcaatgtt 8460
cgaagctcgg tggcttcaca gcagttgaag caaaatctga taaagtaccc aactgttatt 8520
tgggccaatg tgcataaccg ctatcaatca aaaatcaaag tcgaagatga tcaacttgag 8580
gctctttccg ggtcggttta ccctgtcaga ctcgtcgaca gaatcaagag agatatcgac 8640
cgtgaaccaa ggtcaaacgt agatcattac tagccatatg atggagattg gaaaagcaat 8700
aggtctgggt gaagttctac acagaatgaa aagagaaatg atccaggtca gagcactcga 8760
ggactcgcaa gcaagaacga cttcgacagg cctatcaggc ctaaagaagc accaaggtta 8820
tcgaaatata actttaatat tgatgcggct gccatcgtat cagctatcag acgcatcaaa 8880
gataccaaat ggcctcgacc tttacaatcc gatccagccc aaagggatcc taaccaaatg 8940
tgcaaatatc atggcacttc tggccacaga ataaaggatt gtcgacggtt aagagaggaa 9000
gtagcccggt tgttcaataa cgggcacctt caagaatttc tgagcgaccg agccaagaat 9060
cattttagaa atagggattc taacaaatag accgaaccag aagaacctca acacgtcatt 9120
aacatgatca tcggtggagt cgatgcccct caagtgctga tgttgaagcg caccaaagtg 9180
tccattacaa gggaaaaacg gactcgagat tacatattag aaggaacctt gtctttcaac 9240
gacgaggatg cagaagggat cgtgcagcct cacaatgatg cattggtaat atctgtactc 9300
ataaataaat ctcgagttaa gcgtgtgtta attgatccag gtagctcaac caacatcatc 9360
cgattgaggg tcctagaatg gcttggccta caagatcaaa tcatgcctgc agtccgagtt 9420
ctaaatggat tcaacatagc atgcaaaacc actaagggag aaataacatt gccggtgaat 9480
accaccagaa ccatccagga aaccaagttt tatgtgatcg aaggagacat gaggtacaac 9540
gctctgttcg ggaggctaag gatctacagc atgagggcag caccctcgac tcttcaccaa 9600
gtgttaaagt tcccaacgtc gggagggatc aaaacaatct acggggagca accggccgca 9660
aaagaaatat ttgcagtcga agaagagatc ccggtataga cactagcaac atcaaaggaa 9720
ccgagttcgg ataagaaata ataggctaaa tagcaattat cgacaccagc 9770
<210> 59
<211> 9150
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 59
tttaagactg ttttttattt gatttatact ctttaattgt attttcgcac gaaaataacc 60
gatcaaagtt agtcgatttt attaaaaaat aaaattaccg accaaagttg gtcggttttt 120
taaaatgacc ggccgaatta accgaccaat tttggtcggt tttttaatat taatttttat 180
ttattttaat tgaaaaactg accaaaattg gtcggtttct tgaaaaataa atttcacggg 240
actcgaaaat agtttttcgc atttttgctc caaagaaaac cgaccaaagt tggtcgattt 300
cgtaaaaaaa aattaaaaat aaaatatttt aaaaaaccga ccaacttttg tcggtttttt 360
ggtcggtgtt ttgaccgacc aaagttggtc ggtcgacctt ggtcggtttt tgccgaattt 420
ctagtagtga tataccctta gagttacaca attggcacat atatgccctt ctcaaaacga 480
aattcaccca aaaattatgg tttaaacttt aaaataataa aaacatctca aactttaaca 540
atactcaaaa gaccaaaata tttaaattat ttctaaaaag ataatttaat gattaaaagc 600
ctagagttca agttgtagtg ttataaattt gagttgttag tctttttcat cttttttcag 660
ctggacattt tctatttttt ttattaacta tgtaaattag gggtgtacat ggaacgggtt 720
ggatcgattt ttatcaaaac taaaccaaac cgattatatc ggtttgaatt gttcggtttt 780
attggttttt tcagattttt tgttacataa atattatttc aatcttgctt tgttaaattt 840
tttagaacta aatatatgtt cagtaaaact taaaaaattg acaaacatat gatctatctt 900
gattacctta tgggagaatt ttcttagtaa ttggaattca tgagttttgt caagtgaaat 960
tggtgacgaa aatagagaag acatcagtaa ttgaggaaat cggataaggg agaaagaaaa 1020
agaaaaaaag aaaaaaagaa gaaagaaaag agaaaggtaa agaaaaaagc actaataaaa 1080
aggaaatagt atttgtaata tactttaata caattaacgt aagagctaat tagtttgagt 1140
ggattccgtt ttgaaaaggg catacatgtg ccaattatat aactctaagg gcatatatgg 1200
accaactatc tgacggtaag ggcatatttg agttaatata ttaacgaatg acaaatgtgc 1260
tcaatttcgt ataatacaag gacatattac attttcccta ttatgaaatg gttcaaactt 1320
aaggattttg tacatggtaa aacctaaatt gacatgtaac ttggtacttt ccattgggca 1380
aagacacgat cttttacgtg atattttaaa tcaagtaaag atcaagtcgg gccaaaaaga 1440
aaaaaaccca tgatttttta agataaaaag ctgctaactt ttagtttgtt tcatccaata 1500
aaacatcttt aacgatctgt ctgctttagt ttaatcctct ttttaagatg taactaagca 1560
tgaaatagaa aaggggaaaa aaaaggacca ttggattttg gaagaagttt taagaaagta 1620
caagaactag taaagtcatt ttgtatagag tatgttaaaa aggtgagtga caattcgaaa 1680
aagagagagc attgataagt caatcaataa aataaaagca cacctgataa tcattcattc 1740
agaaaacaaa tttctatgaa tgataatcat tatcataagt cactgcagaa atcccatata 1800
cagtagagta ccaggatttt acgataaggt gttagcagac tatctattca ttttttgaca 1860
accattttac gtttggtcat tttttgggaa acgaactctc ccaacattct tccaaattac 1920
cccacgcacc ttactgtgca catcttttaa ccaacttctg gttatttttt cttttgatgt 1980
ccgatattcg tatatgaatt cccattaatt ctaagttgca ccgaaatggt ttttatcaag 2040
attttgtata tatttaatat tcgaattcaa aactaatggt cgaaggtgga agatcgtatc 2100
catcccatca taatatttgg ttggtaatat cacacctttt tgaatttggg agacttgtca 2160
atttttattt tgaaaaaaga aaaaaaaaag aaatagaaac taaaaccata gggaaatgaa 2220
caattttatt ttcactccta cctcatttta tttgtcttga atttttcaat tttgttttga 2280
aacttcttca gtttattttc ttggaatctt cagaatttaa tttgaaattc caaaattcca 2340
aggatttagt gtcaaatcag tgcttgaaat taaatttaaa acgagtggta aataaaatag 2400
aggagaactc ggtaaattac aggagtgcgg taaatctttt ctccttttct ctctttggag 2460
cctactctat tctattgtaa ctaagtaact taactacgaa aaacgtgcct agacttttaa 2520
cttcacaagt ataataaata gaagtcaaat tctttcataa tattgtttcc atcctatcaa 2580
acagactttg cctcactgac tctccttctg agtgtgtctt ttttatgtca tttttagtga 2640
atccaattga tttagagact caaatattcc acatgcgtgt cttaatttgg tgtatatatg 2700
gtaataattt ttgttaggta gctgtagtat tctattattg ttatgtatta actcatgtaa 2760
ataaaagccg gttagataag actagaaaaa atagagtcta cttagaaatt attagcctat 2820
tgtttggaac atgtcaaaaa ttcagtgact cagctagagc tgtcaattag tcaaataact 2880
ttattaatat taacttatga aaacacttgg ggattcttgt agtttaaggg aaagactact 2940
gactgaaaaa caaagcaaaa gtctatgcat tactatatta tacacaatac agcattttcc 3000
aatagtattt tagataaatc tccaatcagc tactgttgtt cttttctttt cttttttagt 3060
ttaagttgta tgtgttgacg gtatacaaat tatttgcaca attagatggc ttatctagat 3120
aatacgtgta aatctattga taatcattaa ttagtaatct ggtaaaaata atattgcttt 3180
tgttctaata taatgtgata tatttgactg ggtacgaaat ttaaaaaaaa ataagacata 3240
tagaacttgt tgtcttaaac aattcataac atttgtgtgg ctataattct tttgaaactt 3300
atggtgttaa acatgtctaa ttgtttgtgt atgtataaaa gattctcatt aagcgtagga 3360
aaatttgaat taaattattt ttttaattta aaaagagatc actcctttta gagctgactt 3420
aaaaagaaat tgattcacat aaactcgcac ggagggaata agtaatatac tatcaaaaat 3480
taaaaatcac ttgtagtgta aaaaaatctt tacaccaatc gtgtatattc tcaatttttt 3540
tttttttttt ggcgagaggt agttgttcag caaaagtaag ttagaaatag gtctgtactt 3600
ttgactttgt aactctgaaa tgaaaaattc aaaatctctt cttttttact gttttaaaaa 3660
ctccaactca ctcttattaa tataaagctc tagttagcaa agacaccctt gtccacttgt 3720
ctatatagca agaaagagag taaaggagaa aacatattct cctctccatt tctgtagaca 3780
agattctcaa aaagaaacaa attaaacact agagagtgag agagaactat aagaaaaaga 3840
atatggggag agctccatgt tgtgataaag caaatgtgaa gagagggcca tggtctcctg 3900
aagaagatgc taaactcaaa gatttcattc acaaatatgg aactggtgga aattggattg 3960
ctcttcccca aaaagcaggt aacaacaact tctactccct tattcccaga atcgaagcga 4020
caaagggtta aatctcagtg gattgtggta gcaagatcat attctatcgc ttacaatatc 4080
tcgtcgcgta tttaacactt tcgtatgtat atgtttgaat atagggggag ggaagcttac 4140
attaatattt atactttgag tggattaagt ttttttttgg ttgcttcatt atttatgatt 4200
ttgatgagat atatgtttgg aactgcagga ctaaagagat gtgggaagag ttgtagattg 4260
agatggctaa attatctaag gcctaatatc aaacatggtg atttttcgga ggaagaagat 4320
agagttattt gcagcttgta ttccaccatt ggaagcaggt acaatatacc tttttttagt 4380
cttaaattgt tttccatttt ttatcatctt tctgatgaat ttgagactga aacaaaaact 4440
gttcccacta aaaatggaaa agaagaacct taataaataa gaaaagggaa aaaacaatga 4500
gggctcagaa agaaatgcaa atagtctgtt ggatttttaa ttaaagattc tgccatttat 4560
ggacattttt ttctgcatgc atgccaggtt tagatctaag atcaagtctt tatttactca 4620
cttacagctg tttaagtatt actactacaa aattccaacg tttcttcttt tctctctttt 4680
tttttttttt tttggaaaac ttttcctttt gtaaaccaat taaattttgt tataacatat 4740
gcaatatatt atgttaacag gtggtcaata atagcagctc aattaccagg aaggactgac 4800
aatgatatca agaattactg gaatactaaa ctcaagaaaa agcttatggg attaatgcaa 4860
tcaacaaacc aaagaaaatc accatatttt ccagctacta attctcttca agcccaaccc 4920
cagataaatt caagtctttt tagagactta tattacaacc caaataatag gcctattatt 4980
acaggcctaa atcagtccat ttcttctgcc caccagccaa attttctcta cactaatagt 5040
aacatgaatt ttcctaattt gggtgctaca aatagtcaat atccttataa tattcaaagt 5100
cataatttac ttatgtttgg agaagcaagt tgttcttcat cagatggaag ttgtagccaa 5160
atgagttttg gcaaagaaat caagagagag gaaattatga gtaattgttt acaacaaggt 5220
caaatttcaa gtgttaatgc ttttgaagaa aatcagaatt tcactcttga ttatggtaac 5280
agtagtagta attgggtgga tcaaaaacca aatgtgtatt ttggaaatac tactactact 5340
actcaagtac ttcagtatga tgttgaagaa gttaagcagc agctaacaag ttgtaccaat 5400
ggcaacaatg gcagtactat tggatgtaac aacaacaaca gtatgttcgt gttcaatgat 5460
gagaattata acaagtcaaa tgagataggg atgttctatt actgaagaag aaatgactag 5520
ctgttgaaaa gagaaaacaa atgtaagtac accattagga agatttgaaa gggcgtttgg 5580
gtatgggggt tggcaagaag attcaaactt tttctggggt tttgtgtaat tgtggtggaa 5640
ttattattat tgaaacttct ttacttcaat ttaaatcgtc ggtacatatt acgtagttgt 5700
agtaaaagcc ttttcctttt tgtgcttttt tttttttttc gtgttcgtat taagacttca 5760
ttaaatccaa atttgcatag ggacggtcaa cattagagga ataaattgct tcctaacaaa 5820
gacgatttta tactcaagag ttcgagcccg aaaaacgacc tctggttaag ggtaaaaata 5880
gtaattacaa taactccacc acaatcctta ttggtgtgca tttcttcatt aaatactccc 5940
tccaatccac tttaattgat ttgtttttgg ctatttttat atatattaag gaattatctt 6000
ttagcattaa tcaataatga aattgaccat attaaccttt tagttcattg gaaatataac 6060
aaatactcct aggcttttta attcaagagc aacttttaaa tccgaatttg ggctaagaat 6120
acaagcttgt tcttttttat ctgtttttca ctcggtgtac gaggactcaa ttaaatccga 6180
atttgagcta agaatacaga cattagaggt aatatgcttt ctaacaaatg tgactcaatg 6240
ttcagactca gaactcgata tctctggtaa ggatgacata gtacttacaa taactccatc 6300
ataatcttta taggtatgta tttctttata aaatatgtaa atagtgttat gattttttgt 6360
atcaaaaatg atgaagtata atactcttaa aaatcatact ccatccgttt caatttatgt 6420
gaacgtattt tctttttagt ctgtgccaaa aagaatgacc tatttcctta tttggaaata 6480
atttaccttt atgcaatgat ttatagtcac acaaaatata tgtgtctcat ttttaaccac 6540
aagttcaaaa gtcttctatc tttttttaaa ctctgtgccc agtcaaatga gttcacataa 6600
attaaaacgg agggaataat aaaaatgtat taaagactac ttaggagagt tcttaaaaaa 6660
ccattttgga accttgtcta cgtactttta tgcaataact gcttaagttt gtctctgcta 6720
aaacctatgc tccccaaccg tgcaccaatc agcttagaaa tttgaactca ggaataaatg 6780
taactacact ccacagaaac ttaaaaagtt ttactgttac cattcactca aggatcagaa 6840
ctgaaaaaca aaagaatcag tgcttcacta aaagaaatac tgtttacatt attttcaaaa 6900
gagtttaatc attaaaatag atgtaccatc agattagcta aaagataaat aatcgttaaa 6960
aaaaggagat tatcaacttg aaaatgaaac aaattatatg ttataatatg tcaaaatata 7020
ctgacagtat aaaaactcgt taaatgtgtt aaatcctatg aaaaaactgc ccaaataaat 7080
atttgagctt aggtgtcaaa tgttgtactc aacaacaata acaacaacgc attaggatcc 7140
tactagtggg gtgtccaatg ttgtactatt gaacattatt caactaactt ttgttaggtg 7200
ttcctgtagt ttagtgaaat taaagtccac tgttccccta tatattaatc ccaaattaat 7260
taatcaagtg cagataaaaa tttctcattt tctattaatt tattaagtgt aacaaactaa 7320
agaaattcaa gaatcttgaa tgatgagaaa gagtcatgca tgtagaaaaa tagataataa 7380
tacatggaaa tatatatgta tttggggatt tgcatggtag ctcaaagatt attggaaagt 7440
gacaggaaga taaatcaaaa tctcagtgtt atttcaaaaa taaaaggcac agattattta 7500
aataattgac agccagtttt ataatactat gtgggagggg acagagatca atccatgtac 7560
gtgcatggct aatattaaag taagggagaa aaaaatatta agttaattga tgattaaaaa 7620
tagtaaaatt tcagacgtat atcacggcaa tgaagagttt gatctttaat atctgtataa 7680
tggtcccata atatgatgga taggcgttgt ttatgatatg attgattgat cattgatcat 7740
tgactattgt ttcttgaata attaatcagt atgggaaagg ggtcccatta aagttgacca 7800
tttgcttagc aatattatct taggtaagct ccatattagt ttaatccact tgcgaatata 7860
ttccgtcctc gcaaatcaat atttacaatt ctttttttca gttttctatc cggtatctga 7920
tacttgcatt ggtgttcgac aaaatctgta ttcgcgtcaa aaaatttcat attatggggc 7980
aaaatgctcc ataataaaag cgactcaata ttagggctcg aaccaatggc ggaaacaaga 8040
tttttactaa gggaattcaa aaaataaaaa cgataaacac atgaagaacc tcagggaatt 8100
caacatctaa tataaatata tgaaataaaa atttgattct attgtaattt gatatacagt 8160
gtaatttaca ccgtagggga tttggctaaa cctccttccg cgtacctagc tccgtccctg 8220
actcgaatcc gaggtatttg gttaaaaatg aaagagtact tctcataacc tcgtcggttt 8280
ttgtttctaa tcaatcttta tattgttaaa acataaaacg tttacttcct ttcttcttct 8340
tttaagtttt gaaaatgata actacttttg tttgactaat attttgtagt ttttgatgct 8400
aatcaatttt gtaaaaatta ctgtacttca actagcgttt actaccccac ctcactttaa 8460
aaaattccct aaagagataa ctttttgatt aattcataaa ctaaattgaa gaacttttca 8520
aatgagagta agttgaaaat gcatattata ttgtagtata taattgcaat tttgcataac 8580
ttaccgtaaa atgttcttcc ttttaatgat ttgttaatat gggaaatttg aacttttctt 8640
tctttgaaat tgtattcttg tcccatggtt tctatgcaat ctcaatcatc aaattgcaat 8700
tatttttttt tgttttttgt tggcaaattc aggagagctt aggtcagtga tatatgaaaa 8760
actatttttt actcttattt attttaccct ttacttatta aagaataaag tccaagacga 8820
atagacgatg tacaacgcaa atgtaaaaat acagaaaaaa tgtttacgac ttcttctcta 8880
tttattttct acttaattta cttattaaac aagtacttac ttgttaaact agctaatctg 8940
accaacaatg tgaaaatgtt tgacattata catcttgact ttttatttct ctattatttt 9000
ctcgatggtt acttcaaatc atagatttgc taatctgacc aatatcgttt aacttcaagt 9060
agaacgaaat gaacatttca aggttttaga aaacagttga aattggaccc taaaataaat 9120
aaaatgaagt tattaatagg tttacacccc 9150
<210> 60
<211> 9227
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 60
gagggtagtc ttgttatatt attggggaat gaattatggt ttcaaacttt tcaaacttaa 60
aggattttgt acatggtaaa acctaaattg acacgtaact tggtactttc aaagacacga 120
tcttttacgc gatattttaa ataaagaaaa gatcaagtca aaacatgggc caaaaagaaa 180
aaccccatga ttttttctga taaaaagctg ctaactttta gtttgtttta tccaataaaa 240
catctttaac ggtctgcctg ctttagttta atcctctttt taagatgtaa ttaagcataa 300
aatagaaaag ggaaaaaaaa ggtccattgg attttggaag aaattttaag aaagtacaag 360
aactagtaaa gtcattttgt atagagtatg ttaaaaaggt gagtgacaat tcgaaaaaga 420
aagcattgat aagtcaatca ctaaataaaa aagcacacct aataatcatt cattcaaaaa 480
aacaaatttc tatgaaagat aatcattatc ataagtcact gcagaaatcc catatacagt 540
agagtaccag gattttacga taaggtgtta gcaaactatc tattcatttt ttgacaagca 600
ttttatgttt ggtcatttgt tgggaaaaat tagggagaaa tttaaaaata gttagattta 660
caactggtca ttaaaaatag cccaatttca aaagtaatcg aaatttagcc acttttcatg 720
taaagataaa tctgagcgaa aatattgttc aaaacccgga aaatacgccc gtatattata 780
ctggagttcc agcataagta tgcttgaact ccagcatatt atacgggagt tctaggataa 840
ctatgttgga actccagcat aatatgttgg agttccagca taagtacact agaactccag 900
catattatac gggagttcca acaagtataa ctgtcccgta taatatattg gagtttggag 960
caccggtgct ccagtctccc gtatattata caggagtcag caaagtatac cggtccagca 1020
taatatgctg gagttcgtac acagatgcac cgaactcacg tatattatgc ggaaccggtc 1080
tctgttgcag caaaatagtg gctatttttc attgacttcg taaacggtgg ctatttttga 1140
atgaccagtc cgaaaactgg ctataccgtg ctattttgac gaaaaattat ccccccaccc 1200
acccacccac ccaaacgcac cttacacaca ttagtgcaca tcttttaact agtttttggt 1260
tattttttta tttgatgccc gatattcgta tatggatttc gattaattag aattcacacc 1320
gaaacattct ttcttaggat tttgtacata cttaatatgc gaatacaaaa cctatgcgga 1380
aaggtaaggg aacctattca tccctctaca gtacttgtga taatgttata cttttttgaa 1440
tttaatttgg gagacatgtc aatctttatt ttgaaaaaaa aaatagaata aaaccatagg 1500
gaaatgaaca atttatcttt cactcctatc tcattttatt tgtcttgaat ttttcaaaat 1560
tttgaattat attttgaaac ttcttcaatt tattttcttg gaatcttcag aattcaattt 1620
aaaattccaa aattccaagg atttagctcc cgtttggcca cagattttgg cttcattttt 1680
ttaaaaaaaa ttttgaaaac attctttgtt tatgcaatat gatcatgttt taggggaaaa 1740
aaattaaaaa aaataaaaaa aaatcaaatt cccaaaaact ggttaggcaa tttttggatg 1800
atattttttc ttccactcac aaaactttaa catgtccaaa cacaacttca acttcaaaaa 1860
ttattttcaa cacaatttta aaaactcttt tttcaagttt caatcaaatc tatatccaaa 1920
tgttagctta gtatcaaata agtgattgaa atcaaattaa aatcgagtgg taaataaaat 1980
agaggagagc tcggtaaatt acaagagtgc ggtaaatctt ttctccttta ctctcactgt 2040
agcctattct atctgttgta actaataagt aactgagcta cggaaaaagt gcctagactt 2100
ttaacttcac aagtataata aatagaagtc aattctttca taatattgtt tccatcctat 2160
caaacagact ttgtctcact gaccttcctt ctgagtgtgt cttttatatg tcatttttag 2220
tgaatccata tgatttagag actctaatat tccacatgcg ggtcttaatt tggtgtatat 2280
gtatatggta ataatttttg ttaggtagct gtagtattct attattgtta tgtattgact 2340
catcatgtaa ataaagccgg ttagataagg ctagaaaaat atgagtatac ctagaaatta 2400
ttagcatatt gtttggaaca tgtcaaaaat ttcaatgacc tagctagagc tgtcaattag 2460
tcaaataact ttattaatat ttacttatga aaacactttg aaattcttgg agtttaaggg 2520
aaagactact gactaaaaaa caaagcaaaa gtctatgcat tactatacta tacacagcac 2580
agcattttcc aatagtattt gagatgaatc tccaatcagc tactgttgtt cttttctttt 2640
ctttatttag tttaagtttt atgtgttgat ggtatacaaa ttatttgcac aatcaaatgg 2700
cttatctgga taatataggt aaacctcttg taatcactaa ttggtaatct ggtaaaaata 2760
acactatttc tattccaatt tatgtgatca atttcactag acaaaaattt aagaaagaaa 2820
taaatttttt agaacttgta gtcataaaca agttgtaaca tttgtatggc tataattttt 2880
ttaacttgtg atgttaaaca tgtcagattg tttgtgtagc tataaaagtt tttcattagg 2940
cgtaaaatta aaaatttaga ttaaattatt attaaattta gaaagaggtc atttttttta 3000
gcgaagtaaa aaagaaatcg gttcacataa accgaaacat agagtaagta atctgttatg 3060
acaaattaaa aattacttgt agtgtaaaaa aatctttaca acattcgtgt atatacttaa 3120
atctttttta ttttttggca agagatagtt gttcagcaaa agtaagttag aaataggtct 3180
gtccttctga ctttgtaact ctgaaatgaa aatttcaaaa tcccttctat ttttactgtt 3240
accccccccc cccctcacaa accccaactc actcttattt aataaaaagc tctacttaga 3300
aaagacaccc ttgtccatct gtctatatag gtagaatgag agtaaaggag aaaacatatc 3360
ctcctctcca tttctgtaga caaagattct caaagagaaa caaattaaac actagagagt 3420
gagagagtgc tataagaaaa agaatatggg gagagctcca tgttgtgata aagcaaatgt 3480
gaagagaggg ccatggtctc ctgaagaaga tgctaaactc aaagatttca ttcacaaata 3540
tggaactggt ggaaattgga ttgctcttcc tcaaaaagct ggtaacaaca acttctactc 3600
cactagtcct ctatgtgtat gtaattttat tattattatt attattatta ttattattat 3660
tattattatt attcatgaat cgaagggaca aaggtctaaa tctcagtggg tcgtggtagc 3720
aaggccattc cgccatttat aatatcttct tgcaaattcc accagtttca tatgtgtatg 3780
tttttttctt attagtcata aatcaaagcg acgaagggtt aaatttcagt tgattgtgat 3840
agcaaggtca cactctaccg cttataatat ctcgtggcgt atttaacatt gtttgtatgt 3900
atatgtttga gtataaaggg aggaaagctt atatttatat ttgagtggat tgagtttttt 3960
tccttgttgc tgcattattt atgatttgat gagatttatg ttgggaactg caggactaaa 4020
gagatgtggg aagagttgta gattgagatg gctaaattat ttaaggccta acattaaaca 4080
tggtgatttt tctgaggaag aagatagagt tatttgcacc ttgtattcca ccattggaag 4140
caggtaatat atatatacct ttttttggtc gtaatttttt tttcattttt tatcatcttt 4200
ctgatgaatt tgagactgaa acaaaaactg ttcccactaa aaatggaaaa gaaaaacctc 4260
aataagtaag aaaagggaaa aaacaatgag ggctcagaaa gaaatgcaaa tagtcagttg 4320
gatttttaat taaagattct gccatttatg gacatatttt tctgcatgca tgccaggttt 4380
agatctaaga tcaagtcttt atttactcac ttacagatgt ttaattatta agacaaagtt 4440
ccaatttttc ttctttcttc tctttctttt tgtggaaatt ttttctctag taaaccaatt 4500
aatttttgtt ataacatgtg caatataata tgttaacagg tggtcaataa tagcagctca 4560
attaccggga agaactgaca atgatatcaa gaattactgg aatactaagc tcaagaaaaa 4620
acctatggga ttaatgcaat caactaacca aagaaaatca ccatattttc cagctactaa 4680
ttctcttcaa acccaacccc agataaattc aagtcttttt agagacttat attacacccc 4740
aaataatagg cctaatatta caggcctaaa tcatcagtcc atttcttctg cccaccagac 4800
aaattttctc tacactaata ataacatgaa ctttcctaat ttgggtgcta caaataatca 4860
atatccttat aatatccaaa gtcataattt acttatgttt ggagaagcaa gttgttcttc 4920
atcagatgga agttgcagcc aaatgagttt tggtaaagaa atcaagagag aagaaattat 4980
gagtaatagt ttacaacaag gtcaaatttc aagtgttaat gcttttgaag aaaaccacca 5040
gaattttact cttgattatg gcaatagtag tagtaattgg gtggatcaaa aaccaaatgt 5100
gtattttggt actactacta ctcaagtact tcagtatgat aatgttgaag aagttaagca 5160
gcagctaaca agttgtacca atggcaacaa tggtagtact attggatgta acaacaacaa 5220
cagtatgttc gtgttcaatg atgagaatta taacaagtca aatgagatag agatgttcta 5280
ttactaaaga agaaatgact gttgaaaaga aaacaaatgc aagtaccatt aggaagattt 5340
gaaagggcgt ttgggtatgg gggttgccaa gaagattcag actttttttg gggttttgtg 5400
tagttgtggt agaattatta ttgaatgaaa aaaaaaaact tcctgtactt taattcgtca 5460
gtacatacta catactacta caaagtagtt aaaagcctat tctatttgtg cttttttttt 5520
cactcgatgt ccaataatta tattggtttt tgattaaatt tgaatttgag caaggaagat 5580
caacattgga gggataaatt gtttcctaac gaaggcgatt acatacttag aacttgaact 5640
caatatctct aattaaaaat gaagtaatac ttataataac tccaccacaa ttcttattgt 5700
tgtgcatttc tttataaaat atgtaaataa tgggtcatat atattgttta ccttttctat 5760
tcatatacat agattttaaa ttaattatac acatatatat aatacattaa ttattcatat 5820
attatatttt tgctagctat ttttagttta agcgatttgg taggcgacta cttgggttaa 5880
ttcttttttt ttaatatata tatcaaaata atgaagctgt ataatacact taaaaatcat 5940
atttgaaagg tattaaatac gacttaggag agttcttaaa ccattttgga accttgtcta 6000
cgtactttta tgcaatagct gtttttgttt gtctctgcta aaacctatgc tccccaaccg 6060
tgcaccaatc aacttagaag ttagaactca gaaataaatg taactatact ccacagaaag 6120
ttaaaaagtt ttactgttac cattcactca aggatcagaa actgaaagac aaatgaatca 6180
gtgcttcact gttcttcact aaaagaaata ctgtttacat tagtttcaaa agagtttaat 6240
cataaaaaca aatgtaccat aaaaagggga gattatcaac ctgaaaatga aacagaacat 6300
acgttatata tcaatctata tacggtcgag atcggactcg tctattacac gacagatcgg 6360
gattgaaacg taacagtttt gaagatcaac cccgggttcc gtcggaccga ggtacaaggt 6420
cagaatgccc gttctcgaga acatcgagtc catgacccca gaatcaaccc tgaccccaaa 6480
tgagctcgag gaaacatccg gataacggaa ggcgaaatat ccgtaaccgg tcgggtatca 6540
cggcatgaat ttcggcacgt aacaatgaga aaccggctaa ttagcaaatc atggaatttt 6600
ttacctttta tagaattgta actaaagtgg gattccccta ctatgtaaag ggggtctgac 6660
tatttgtacg ggacattcat taaacgcatc ccaaagtaat ataatattat tttctttttg 6720
taagctattg ttctcctgta tctgatacta tttgaattgc atcaagttca agtgagactc 6780
attttttcaa ggctataatt gttcaagtcg cacggtttga atttattcga tcattgttcg 6840
ctttaattac aattcaattc atcgctttat gtcaaattaa tccacatatc cttaaaacca 6900
cttacaaatt taattgttat caaattttaa gggtaaacag tttggcgctc accgtggagc 6960
taaggataat agtggttgtt tgatatagat tttcataaca cacactattt tacaattgtt 7020
cttcgaagtg tctctcattt caggtttaag ctcaaaatgt caaactcaca attggcaccc 7080
ctacctgcac acaatgagtc tggtcaccat ggtgaaaata acaacatagc acctggtaac 7140
gaggtaccgc ccgctgatcc catcagaatt tcaatcgcgg acccgttgga cgctaactcg 7200
catgtggcta tcgacatgtt acagtctcaa caggcgacga tagctcagtt acaaaaccaa 7260
agccgcacac cgagcagagt tgaactcgat ccgtcccgga aaatcacctg cagggaagaa 7320
ccgtccgcgg agaggtcaaa tggagatgag tcggggacta accccgagat cataaaaatg 7380
cttgaggaac cgatgatacg gattgaatca ggggaaaaga aaatcgaggc aaatgacaag 7440
aaggtaaaaa cttacaattt cacggtcaac caaatcccgg gagcaccgcc ggtactgaaa 7500
agcttggatt ccaagaagtt cgtgcaaaaa catttccctc cgagtgtggc cccgaaatcg 7560
atcccaaaaa catttatatg cccgagattc ttaagtataa tgggacaacc gacccaaacg 7620
agtatgtcac ttcttacaca tgccctatca aagggaacaa cttagaggtt gatgagatcg 7680
agtctgtttt gttgaagaaa ttcggagaga ccctgtcaaa tggagctatg atatggtatc 7740
acttacctcc taattctatt gactcatttg caatgcttgc aaactctttc gtgaaagcac 7800
acgccagggc tatcaaggtc gagacccaga agtcggacct cttcaaagta agacagaagg 7860
ataatgagat gctcaaagag tccgtgtcct agtttcaaat gaaacagaag gacctaccac 7920
cggtcgctga tgattgggcc gttcaagctt tcacccaagg actcaatgtt cgaagctcgg 7980
tggcttcaca gcagttgaag caaaatctga taaagtaccc aactgttatt tgggccaatg 8040
tgcataaccg ctatcaatca aaaatcaaag tcgaagatga tcaacttgag gctctttccg 8100
ggtcggttta ccctgtcaga ctcgtcgaca gaatcaagag agatatcgac cgtgaaccaa 8160
ggtcaaacgt agatcattac tagccatatg atggagattg gaaaagcaat aggtctgggt 8220
gaagttctac acagaatgaa aagagaaatg atccaggtca gagcactcga ggactcgcaa 8280
gcaagaacga cttcgacagg cctatcaggc ctaaagaagc accaaggtta tcgaaatata 8340
actttaatat tgatgcggct gccatcgtat cagctatcag acgcatcaaa gataccaaat 8400
ggcctcgacc tttacaatcc gatccagccc aaagggatcc taaccaaatg tgcaaatatc 8460
atggcacttc tggccacaga ataaaggatt gtcgacggtt aagagaggaa gtagcccggt 8520
tgttcaataa cgggcacctt caagaatttc tgagcgaccg agccaagaat cattttagaa 8580
atagggattc taacaaatag accgaaccag aagaacctca acacgtcatt aacatgatca 8640
tcggtggagt cgatgcccct caagtgctga tgttgaagcg caccaaagtg tccattacaa 8700
gggaaaaacg gactcgagat tacatattag aaggaacctt gtctttcaac gacgaggatg 8760
cagaagggat cgtgcagcct cacaatgatg cattggtaat atctgtactc ataaataaat 8820
ctcgagttaa gcgtgtgtta attgatccag gtagctcaac caacatcatc cgattgaggg 8880
tcctagaatg gcttggccta caagatcaaa tcatgcctgc agtccgagtt ctaaatggat 8940
tcaacatagc atgcaaaacc actaagggag aaataacatt gccggtgaat accaccagaa 9000
ccatccagga aaccaagttt tatgtgatcg aaggagacat gaggtacaac gctctgttcg 9060
ggaggctaag gatctacagc atgagggcag caccctcgac tcttcaccaa gtgttaaagt 9120
tcccaacgtc gggagggatc aaaacaatct acggggagca accggccgca aaagaaatat 9180
ttgcagtcga agaagagatc ccggtataga cactagcaac atcaaag 9227
<210> 61
<211> 9159
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 61
agactgtttt ttatttgatt tatactcttt aattgtattt tcgcacgaaa ataaccgatc 60
aaagttagtc gattttatta aaaaataaaa ttaccgacca aagttggtcg gttttttaaa 120
atgaccggcc gaattaaccg accaattttg gtcggttttt taatattaat ttttatttat 180
tttaattgaa aaactgacca aaattggtcg gtttcttgaa aaataaattt cacgggactc 240
gaaaatagtt tttcgcattt ttgctccaaa gaaaaccgac caaagttggt cgatttcgta 300
aaaaaaaatt aaaaataaaa tattttaaaa aaccgaccaa cttttgtcgg ttttttggtc 360
ggtgttttga ccgaccaaag ttggtcggtc gaccttggtc ggtttttgcc gaatttctag 420
tagtgatata cccttagagt tacacaattg gcacatatat gcccttctca aaacgaaatt 480
cacccaaaaa ttatggttta aactttaaaa taataaaaac atctcaaact ttaacaatac 540
tcaaaagacc aaaatattta aattatttct aaaaagataa tttaatgatt aaaagcctag 600
agttcaagtt gtagtgttat aaatttgagt tgttagtctt tttcatcttt tttcagctgg 660
acattttcta ttttttttat taactatgta aattaggggt gtacatggaa cgggttggat 720
cgatttttat caaaactaaa ccaaaccgat tatatcggtt tgaattgttc ggttttattg 780
gttttttcag attttttgtt acataaatat tatttcaatc ttgctttgtt aaatttttta 840
gaactaaata tatgttcagt aaaacttaaa aaattgacaa acatatgatc tatcttgatt 900
accttatggg agaattttct tagtaattgg aattcatgag ttttgtcaag tgaaattggt 960
gacgaaaata gagaagacat cagtaattga ggaaatcgga taagggagaa agaaaaagaa 1020
aaaaagaaaa aaagaagaaa gaaaagagaa aggtaaagaa aaaagcacta ataaaaagga 1080
aatagtattt gtaatatact ttaatacaat taacgtaaga gctaattagt ttgagtggat 1140
tccgttttga aaagggcata catgtgccaa ttatataact ctaagggcat atatggacca 1200
actatctgac ggtaagggca tatttgagtt aatatattaa cgaatgacaa atgtgctcaa 1260
tttcgtataa tacaaggaca tattacattt tccctattat gaaatggttc aaacttaagg 1320
attttgtaca tggtaaaacc taaattgaca tgtaacttgg tactttccat tgggcaaaga 1380
cacgatcttt tacgtgatat tttaaatcaa gtaaagatca agtcgggcca aaaagaaaaa 1440
aacccatgat tttttaagat aaaaagctgc taacttttag tttgtttcat ccaataaaac 1500
atctttaacg atctgtctgc tttagtttaa tcctcttttt aagatgtaac taagcatgaa 1560
atagaaaagg ggaaaaaaaa ggaccattgg attttggaag aagttttaag aaagtacaag 1620
aactagtaaa gtcattttgt atagagtatg ttaaaaaggt gagtgacaat tcgaaaaaga 1680
gagagcattg ataagtcaat caataaaata aaagcacacc tgataatcat tcattcagaa 1740
aacaaatttc tatgaatgat aatcattatc ataagtcact gcagaaatcc catatacagt 1800
agagtaccag gattttacga taaggtgtta gcagactatc tattcatttt ttgacaacca 1860
ttttacgttt ggtcattttt tgggaaacga actctcccaa cattcttcca aattacccca 1920
cgcaccttac tgtgcacatc ttttaaccaa cttctggtta ttttttcttt tgatgtccga 1980
tattcgtata tgaattccca ttaattctaa gttgcaccga aatggttttt atcaagattt 2040
tgtatatatt taatattcga attcaaaact aatggtcgaa ggtggaagat cgtatccatc 2100
ccatcataat atttggttgg taatatcaca cctttttgaa tttgggagac ttgtcaattt 2160
ttattttgaa aaaagaaaaa aaaaagaaat agaaactaaa accataggga aatgaacaat 2220
tttattttca ctcctacctc attttatttg tcttgaattt ttcaattttg ttttgaaact 2280
tcttcagttt attttcttgg aatcttcaga atttaatttg aaattccaaa attccaagga 2340
tttagtgtca aatcagtgct tgaaattaaa tttaaaacga gtggtaaata aaatagagga 2400
gaactcggta aattacagga gtgcggtaaa tcttttctcc ttttctctct ttggagccta 2460
ctctattcta ttgtaactaa gtaacttaac tacgaaaaac gtgcctagac ttttaacttc 2520
acaagtataa taaatagaag tcaaattctt tcataatatt gtttccatcc tatcaaacag 2580
actttgcctc actgactctc cttctgagtg tgtctttttt atgtcatttt tagtgaatcc 2640
aattgattta gagactcaaa tattccacat gcgtgtctta atttggtgta tatatggtaa 2700
taatttttgt taggtagctg tagtattcta ttattgttat gtattaactc atgtaaataa 2760
aagccggtta gataagacta gaaaaaatag agtctactta gaaattatta gcctattgtt 2820
tggaacatgt caaaaattca gtgactcagc tagagctgtc aattagtcaa ataactttat 2880
taatattaac ttatgaaaac acttggggat tcttgtagtt taagggaaag actactgact 2940
gaaaaacaaa gcaaaagtct atgcattact atattataca caatacagca ttttccaata 3000
gtattttaga taaatctcca atcagctact gttgttcttt tcttttcttt tttagtttaa 3060
gttgtatgtg ttgacggtat acaaattatt tgcacaatta gatggcttat ctagataata 3120
cgtgtaaatc tattgataat cattaattag taatctggta aaaataatat tgcttttgtt 3180
ctaatataat gtgatatatt tgactgggta cgaaatttaa aaaaaaataa gacatataga 3240
acttgttgtc ttaaacaatt cataacattt gtgtggctat aattcttttg aaacttatgg 3300
tgttaaacat gtctaattgt ttgtgtatgt ataaaagatt ctcattaagc gtaggaaaat 3360
ttgaattaaa ttattttttt aatttaaaaa gagatcactc cttttagagc tgacttaaaa 3420
agaaattgat tcacataaac tcgcacggag ggaataagta atatactatc aaaaattaaa 3480
aatcacttgt agtgtaaaaa aatctttaca ccaatcgtgt atattctcaa tttttttttt 3540
ttttttggcg agaggtagtt gttcagcaaa agtaagttag aaataggtct gtacttttga 3600
ctttgtaact ctgaaatgaa aaattcaaaa tctcttcttt tttactgttt taaaaactcc 3660
aactcactct tattaatata aagctctagt tagcaaagac acccttgtcc acttgtctat 3720
atagcaagaa agagagtaaa ggagaaaaca tattctcctc tccatttctg tagacaagat 3780
tctcaaaaag aaacaaatta aacactagag agtgagagag aactataaga aaaagaatat 3840
ggggagagct ccatgttgtg ataaagcaaa tgtgaagaga gggccatggt ctcctgaaga 3900
agatgctaaa ctcaaagatt tcattcacaa atatggaact ggtggaaatt ggattgctct 3960
tccccaaaaa gcaggtaaca acaacttcta ctcccttatt cccagaatcg aagcgacaaa 4020
gggttaaatc tcagtggatt gtggtagcaa gatcatattc tatcgcttac aatatctcgt 4080
cgcgtattta acactttcgt atgtatatgt ttgaatatag ggggagggaa gcttacatta 4140
atatttatac tttgagtgga ttaagttttt ttttggttgc ttcattattt atgattttga 4200
tgagatatat gtttggaact gcaggactaa agagatgtgg gaagagttgt agattgagat 4260
ggctaaatta tctaaggcct aatatcaaac atggtgattt ttcggaggaa gaagatagag 4320
ttatttgcag cttgtattcc accattggaa gcaggtacaa tatacctttt tttagtctta 4380
aattgttttc cattttttat catctttctg atgaatttga gactgaaaca aaaactgttc 4440
ccactaaaaa tggaaaagaa gaaccttaat aaataagaaa agggaaaaaa caatgagggc 4500
tcagaaagaa atgcaaatag tctgttggat ttttaattaa agattctgcc atttatggac 4560
atttttttct gcatgcatgc caggtttaga tctaagatca agtctttatt tactcactta 4620
cagctgttta agtattacta ctacaaaatt ccaacgtttc ttcttttctc tctttttttt 4680
tttttttgga aaacttttcc ttttgtaaac caattaaatt ttgttataac atatgcaata 4740
tattatgtta acaggtggtc aataatagca gctcaattac caggaaggac tgacaatgat 4800
atcaagaatt actggaatac taaactcaag aaaaagctta tgggattaat gcaatcaaca 4860
aaccaaagaa aatcaccata ttttccagct actaattctc ttcaaaccca accccagata 4920
aattcaagtc tttttagaga cttatattac aacccaaata ataggcctat tattacaggc 4980
ctaaatcagt ccatttcttc tgcccaccag ccaaattttc tctacactaa tagtaacatg 5040
aattttccta atttgggtgc tacaaatagt caatatcctt ataatattca aagtcataat 5100
ttacttatgt ttggagaagc aagttgttct tcatcagatg gaagttgtag ccaaatgagt 5160
tttggcaaag aaatcaagag agaggaaatt atgagtaatt gtttacaaca aggtcaaatt 5220
tcaagtgtta atgcttttga agaaaatcag aatttcactc ttgattatgg taacagtagt 5280
agtaattggg tggatcaaaa accaaatgtg tattttggaa atactactac tactactcaa 5340
gtacttcagt atgatgttga agaagttaag cagcagctaa caagttgtac caatggcaac 5400
aatggcagta ctattggatg taacaacaac aacagtatgt tcgtgttcaa tgatgagaat 5460
tataacaagt caaatgagat agggatgttc tattactgaa gaagaaatga ctagctgttg 5520
aaaagagaaa acaaatgtaa gtacaccatt aggaagattt gaaagggcgt ttgggtatgg 5580
gggttggcaa gaagattcaa actttttctg gggttttgtg taattgtggt ggaattatta 5640
ttattgaaac ttctttactt caatttaaat cgtcggtaca tattacgtag ttgtagtaaa 5700
agccttttcc tttttgtgct tttttttttt ttcgtgttcg tattaagact tcattaaatc 5760
caaatttgca tagggacggt caacattaga ggaataaatt gcttcctaac aaagacgatt 5820
ttatactcaa gagttcgagc ccgaaaaacg acctctggtt aagggtaaaa atagtaatta 5880
caataactcc accacaatcc ttattggtgt gcatttcttc attaaatact ccctccaatc 5940
cactttaatt gatttgtttt tggctatttt tatatatatt aaggaattat cttttagcat 6000
taatcaataa tgaaattgac catattaacc ttttagttca ttggaaatat aacaaatact 6060
cctaggcttt ttaattcaag agcaactttt aaatccgaat ttgggctaag aatacaagct 6120
tgttcttttt tatctgtttt tcactcggtg tacgaggact caattaaatc cgaatttgag 6180
ctaagaatac agacattaga ggtaatatgc tttctaacaa atgtgactca atgttcagac 6240
tcagaactcg atatctctgg taaggatgac atagtactta caataactcc atcataatct 6300
ttataggtat gtatttcttt ataaaatatg taaatagtgt tatgattttt tgtatcaaaa 6360
atgatgaagt ataatactct taaaaatcat actccatccg tttcaattta tgtgaacgta 6420
ttttcttttt agtctgtgcc aaaaagaatg acctatttcc ttatttggaa ataatttacc 6480
tttatgcaat gatttatagt cacacaaaat atatgtgtct catttttaac cacaagttca 6540
aaagtcttct atcttttttt aaactctgtg cccagtcaaa tgagttcaca taaattaaaa 6600
cggagggaat aataaaaatg tattaaagac tacttaggag agttcttaaa aaaccatttt 6660
ggaaccttgt ctacgtactt ttatgcaata actgcttaag tttgtctctg ctaaaaccta 6720
tgctccccaa ccgtgcacca atcagcttag aaatttgaac tcaggaataa atgtaactac 6780
actccacaga aacttaaaaa gttttactgt taccattcac tcaaggatca gaactgaaaa 6840
acaaaagaat cagtgcttca ctaaaagaaa tactgtttac attattttca aaagagttta 6900
atcattaaaa tagatgtacc atcagattag ctaaaagata aataatcgtt aaaaaaagga 6960
gattatcaac ttgaaaatga aacaaattat atgttataat atgtcaaaat atactgacag 7020
tataaaaact cgttaaatgt gttaaatcct atgaaaaaac tgcccaaata aatatttgag 7080
cttaggtgtc aaatgttgta ctcaacaaca ataacaacaa cgcattagga tcctactagt 7140
ggggtgtcca atgttgtact attgaacatt attcaactaa cttttgttag gtgttcctgt 7200
agtttagtga aattaaagtc cactgttccc ctatatatta atcccaaatt aattaatcaa 7260
gtgcagataa aaatttctca ttttctatta atttattaag tgtaacaaac taaagaaatt 7320
caagaatctt gaatgatgag aaagagtcat gcatgtagaa aaatagataa taatacatgg 7380
aaatatatat gtatttgggg atttgcatgg tagctcaaag attattggaa agtgacagga 7440
agataaatca aaatctcagt gttatttcaa aaataaaagg cacagattat ttaaataatt 7500
gacagccagt tttataatac tatgtgggag gggacagaga tcaatccatg tacgtgcatg 7560
gctaatatta aagtaaggga gaaaaaaata ttaagttaat tgatgattaa aaatagtaaa 7620
atttcagacg tatatcacgg caatgaagag tttgatcttt aatatctgta taatggtccc 7680
ataatatgat ggataggcgt tgtttatgat atgattgatt gatcattgat cattgactat 7740
tgtttcttga ataattaatc agtatgggaa aggggtccca ttaaagttga ccatttgctt 7800
agcaatatta tcttaggtaa gctccatatt agtttaatcc acttgcgaat atattccgtc 7860
ctcgcaaatc aatatttaca attctttttt tcagttttct atccggtatc tgatacttgc 7920
attggtgttc gacaaaatct gtattcgcgt caaaaaattt catattatgg ggcaaaatgc 7980
tccataataa aagcgactca atattagggc tcgaaccaat ggcggaaaca agatttttac 8040
taagggaatt caaaaaataa aaacgataaa cacatgaaga acctcaggga attcaacatc 8100
taatataaat atatgaaata aaaatttgat tctattgtaa tttgatatac agtgtaattt 8160
acaccgtagg ggatttggct aaacctcctt ccgcgtacct agctccgtcc ctgactcgaa 8220
tccgaggtat ttggttaaaa atgaaagagt acttctcata acctcgtcgg tttttgtttc 8280
taatcaatct ttatattgtt aaaacataaa acgtttactt cctttcttct tcttttaagt 8340
tttgaaaatg ataactactt ttgtttgact aatattttgt agtttttgat gctaatcaat 8400
tttgtaaaaa ttactgtact tcaactagcg tttactaccc cacctcactt taaaaaattc 8460
cctaaagaga taactttttg attaattcat aaactaaatt gaagaacttt tcaaatgaga 8520
gtaagttgaa aatgcatatt atattgtagt atataattgc aattttgcat aacttaccgt 8580
aaaatgttct tccttttaat gatttgttaa tatgggaaat ttgaactttt ctttctttga 8640
aattgtattc ttgtcccatg gtttctatgc aatctcaatc atcaaattgc aattattttt 8700
ttttgttttt tgttggcaaa ttcaggagag cttaggtcag tgatatatga aaaactattt 8760
tttactctta tttattttac cctttactta ttaaagaata aagtccaaga cgaatagacg 8820
atgtacaacg caaatgtaaa aatacagaaa aaatgtttac gacttcttct ctatttattt 8880
tctacttaat ttacttatta aacaagtact tacttgttaa actagctaat ctgaccaaca 8940
atgtgaaaat gtttgacatt atacatcttg actttttatt tctctattat tttctcgatg 9000
gttacttcaa atcatagatt tgctaatctg accaatatcg tttaacttca agtagaacga 9060
aatgaacatt tcaaggtttt agaaaacagt tgaaattgga ccctaaaata aataaaatga 9120
agttattaat aggtttacac cccaatctta tctaatgct 9159
<210> 62
<211> 6522
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 62
agaacacatg agttctggta ttcttggact ccgctcaatc ttccattcaa caacagtcct 60
gaggttaaac atgtacctgg gtagagatgt aaaggactta tcccagttac cataaacaac 120
ttcaaacgca cgtttgctcc cgagaaatgc cttttctttt ggtagtggta cacccatata 180
cctggtggac aaatgttata tactctttga tcttgtacct tatggacgct tcaatgtgtg 240
gaatcaagat aagagaaatc aagtcaatat tcaagttaaa atgatgccca ctaaatatgc 300
ccatttcaca attgtgggtg ccaatatatt tacccacaac tcatatattt gttttctgct 360
tcctcgcacg cagcatccag ttacaacctg taaatcatct acggaaaata accttgtata 420
catccggaga tgactcatga accatgatct aacgacactc ttttatgttg tacattcgca 480
ccccctgctt aggcacgctt tatcagcaaa aacatgcctt ttgacagcac tgttactcta 540
tattgatccc atattgatgt ccgaatttta tcaagatccc ttgtgagggc atccacatcc 600
gacatactgg gcaaatgatc aaggtgggga atctcccttg aatgaaacgg cacgtgggac 660
tcgtacgctc ttggtctaat gggaggtgga gcatgctccc ttgtcaaatc tggttcaaca 720
ttctcttcct ccccatcatc atcctcatca gggaagggtg tgtcatctcc agactcatca 780
gcattgttgt catatcacta ttttcttcct gactctacgc atctgccaga tcctgattaa 840
atatgtcgtc ttcgggcaat tgagtaagga cataaccttc aagttactcg ttttcattgc 900
acaatcaata aaataatgag tttcataaat tgatccaaat tttacatata gatttatcac 960
ttcacttaca aatcataatg tgttgatatc ccatgatgga cattgttgtg ttgatgatga 1020
ctcccaaatg gaccaccatg atccaacata ctaaaactag gcatatttca actgggcgtt 1080
ggttcataac ttgtaaaatt catatctggc cagtaccccc tgtgaaatat atgggtatta 1140
atataaattc aatatataaa aataaacatc gtaacacaaa aggaaattga aatttaccac 1200
tcggcttgtg gattatgtaa actaggagag aaattatttc ctcgctcctc attagcccgt 1260
tgagataagt ttagatcagg ccaaactctt tcactcggaa tctgttcggc taaaactgct 1320
acaaaataac cacccgatga ttgagggata tccctacttt gcggaacctc attattgtga 1380
acatcttcag ctttgacgta catttccaat atttttatca taagaaattt attcttccgg 1440
agtcctcaaa aaatctctca gagtttcatg gtcttcgatg ttaaactcag cataacaagc 1500
aacccattgt agagtgacag aatacggata tttttcggtt actttaatat tcaccgaacg 1560
tttgctcata ctcatttttt ttacataaca acgatatcaa tgtatcatac tcaattgtaa 1620
gtgacaactt aacatgatac tgtggaaaac aactatagct tattcgtcac cataacctca 1680
cccccaatat aatgaaaccc taattcttcg cttttcaaat attatgacaa aatacaaaat 1740
aacttaatga ataattattt cagacgactt gaaggatcct gaatggattt ttacaaaatc 1800
ctgaaactct ttaaataagg aaaaaaaatc agtcccgggg gcacaattat ttgaggtata 1860
gctccttgaa tatggacgct atacccagtt gaattattgg tgtgctagtt aaatcaagaa 1920
gaattatttg tgggcccaat aaacaccgca ctatacctta acggcaaacg taccgttaag 1980
gtatagcgcg gtaaaatacg acactatata tattatggtg cctaattttt tttctacaca 2040
ttataattct ttgatcgagt ccaaaagccg gactcttatt cctaatgggt atggtgtgtc 2100
ggctatgaat ggacaaaaga gaggatttgg ttaaattccc aaggaatgtc taatatgtca 2160
actacgaaga gaataacaga attactactg ctaattaaag tacttttagg ttgaaaaagc 2220
aaaaagaagt ttgaaatatt agagcacagc actaatattc atgcaaattt tgcacttcaa 2280
aaagaagagc taataaagaa aaagaaggca ctttgttctg actatatcca agaagatatt 2340
ctacagtgat tctcctttta accaaacaaa atgaatccta ggaacagaaa caaaacataa 2400
gccccaaagg ttaattggtt ccggaggtga cataatcaac gacgttcata tatttacccc 2460
tatttcagtc gcatctctga ggaggaaatg gtgttcttct atgttagttt ttaccgattt 2520
ctcgtagaaa gtgtcatgta tagctcaaaa acatttctaa cgagtcttaa aaaccaatct 2580
cccggtatcc tttcctcttc ctttttcttt tcgtttcgca ttcagtgtaa gtatcaattt 2640
tggtctgttt aatttggatt cgtgctaata ttcattttcg gttaaagtca gaggcgaatc 2700
caaactttta agcttatgag ttcctataat aatcccaagt taatctacaa gataactgga 2760
caaatgaact gggctaaata ttcgtatatt tttaatgaat tttttagtat aaatacaggg 2820
tctatgcaaa aattactggg ttcacgagaa cccgtaccgt ttgctctaca tcggctcctg 2880
gttaaagtgc tcctttaatt tgcactccat ttgatatgaa aactttcgtt ttcgactttt 2940
cacaattatt tgacaacact ctgctaacga gttataaagt gaagttctct gtatgaagag 3000
gatagattaa gcttttcata tgtcaaatga atatcttaaa tttctggtaa aatttaaatt 3060
ccatcgataa gaaaaatata aaatgcaatg ggaacagtaa ttttttgtat ttaatttggc 3120
ccctagctag acagtagcac tttcatatgt agcaacttag aattcagtgc tttctcatgt 3180
cccttccagt agtagtgtga aaggacactt aaactattca tttaacctaa tctcagttta 3240
ataatggagt actcagtttc aattctctct tttttttttt cccccccttt ctccatttta 3300
ggtacatggg actattatct aattactgat cgagacattt ttgtatatct gtatatgtcg 3360
aaactgacaa aaagatgact tcgcatgtta agttaaaata ttgcatctaa caaggtggaa 3420
atattatttt ttggtaagag attaagttta tgtattaaca atataaaaaa tatttatatc 3480
aggacagtta aaaagatata tgtattaatt ggtagagttt aactagtatc catcacccat 3540
agaaatattt tacacaatca agttatcttt gtttgttgta acgaaacaac ttatttaatt 3600
tattttcaaa attataaatt tcacttttaa agagaattac atgtaaatga tttctgaatt 3660
acctgatggt gtaaaatatt aagtcatatt atatatctat aaagaaaaaa aaaatgagga 3720
gatgaggcaa aacatcctac aatccttgcg tatacagaaa tacttttacg tactgtcagt 3780
aatattaggt aattttcaat ggcagacctc ttttgttgcc ctattgaccc tacaattgga 3840
ggggtattta ccccccaaga aactcgtaat cttgccctaa agattggctg actcaaatca 3900
gatgaccata tttctatatt gtccgacgta cctaacgcaa tcttcttcct ctatataaac 3960
catgcatgga caatctcatc ttctcaaact tcataaagat atctttaaaa aaaagagaaa 4020
atagaggtaa ttagttgtat caatggatca acaacattcc acttgttttt cttcttcaag 4080
taaaattaat gacaaagaaa agaagaaaaa aagatcagtt gtgaaactat caactgatcc 4140
acaaagtgta gcagctcgtg aaagaaggca tagaatcagt gatcgtttca agattttgca 4200
gagtttaatc cctggtggtt caaaaatgga tacagttact atgttagaag aagcaattca 4260
ctatgtcaaa tttcttaaga ctcaaatatg gctgcatcaa accgtgatta atattgtaga 4320
tgattatgat aatccaaatt atcatgatca gttgctaatg gctcatgact ctaattttgc 4380
taattattat cctcatgaaa tggtggaata ttgcccagct cctgttgaga atgcacaaat 4440
aaattataac ttggaccagc tgcagcttcc aggttatgca ttttcagatg gggatcaatt 4500
ccaaggagaa gaaactaata ttactggtga ttcttttatg tactattagt tagttaatta 4560
tgttgcctaa gtttaattag aatacgtagt gtgtggtagt atggtatgtt gttttctctc 4620
tttctatcta gcagcctaaa gatgggtttg tgttaattaa ttagatgtag taaattgtat 4680
gtgttggtta gttgattaag tatgttgcaa gttattttca ctgatcaatt aatcaatgac 4740
tttgtgaaga agcatctgga agttcaattt gactaatgta taactttttt ttaaaaaaca 4800
atgaatggat aaaaagagtg aaaagaaaga aagagagaaa attatacaaa caaaattacg 4860
ggaggtttag aaacaattta agtactacct gatactataa agaatattta cacaattaaa 4920
tcacttaaaa gaaatttggt atgtgattta gcagtttagc tattatgtag tgaactgttt 4980
ggctaaactt ttaataattg ttcattttta gaattgcttt tatcaaaata acttttcgta 5040
gaggtacttc cggagagaat taaatgcatc tttatttatc atataaggat aatatttgaa 5100
ttaaatagaa gaattaaccc aaatcgtcgt ccgcctaatc tctaaaacta aaaatagcca 5160
acgaatatat atatatatat atatgtgtgt gtgtgtgtaa ctttgtataa tcaatatata 5220
atatatgtat accgtccaaa aaaagtaaat aatgaatctg gccggttatt tatgtaataa 5280
tccggtaaat agaccgtggg cctaaagctg ctgatagagg cctgaaaaca tgggcctatg 5340
tctgctgaaa gcccgttaaa tacgccaacc taaaatagtg cttccttatc cttaaaaagc 5400
aatttgaggt cgtctaaatc aggatggtga ttttatggtt tctctgcatg tcaagaacat 5460
ataggtaaga cacattttta ggagatcact gagataaact gaaacataat ctttctcgtg 5520
tgtttgaaag tagaagaaaa gattactgta gacggtcaaa atcgtgtgtc cccgattttg 5580
taggctcgag gggtcgcatc gagaacaagt tcaatataga ctgagctcga gttcgaaggc 5640
agaatgcgag actcgaagat ctatgtgctc gaggaacatc agagccgaat atggctaatc 5700
tcgagataat accgttatgg ttttgtaaca gaaagggcga gattcccacg gtggccctaa 5760
gatcgtggcg taaattccgg aatagatttg tacgagttag tacggatttg tactaggagg 5820
ttagacagtt gtcccaataa gattctttac tgtaaataga aatgtacatt atttagggtt 5880
cctctactat ataaagggga caccaatcat ttgtaacatt catttaatca ttggcaaaag 5940
aatatactct cttactttct tgttcattac tcatcagaat tgtctcttaa ctttattgtc 6000
tttattttac tattcttgtt gacctacctc gaggtcacca tagctcgagg tcaagacttg 6060
cttaagcact ggtttgattc agcttacttc tttaattttc acgtttgatt tcttggttat 6120
taattagtat tgaactaaat cacgtatctt taaaatcaca aacaagttta attgttactc 6180
gtattttcga ggtaaacagt ttggcgccca ccatggggct aaagataata gtggttattt 6240
cagtactgat tctgataaca cacgttattt ttacacttat tcttttcaag aatttttgtt 6300
ctcaagttaa attatgtcaa actcacaaaa cgcacccgta cacggcgatg atggtctagg 6360
atttcatggg gaaaacaaca atgtagtttg tcacacctcc ctttttccta taccctgtaa 6420
agggtataag ggagtttttc caattaaagg acaatcgaaa cgggattcct tattaatttc 6480
agagtcgcca cctgggaact ttatggcgtc ccaagtcacc ga 6522
<210> 63
<211> 8524
<212> DNA
<213> 红花烟草(Nicotiana tabacum)
<400> 63
agtgattatt agcccaatca cttaaaaact cccgaaggtg cccttcattg aataaccggg 60
ctacttcttc tctcaacttc ctgcaatctt ccattctgtg gccatgggtt ccataatact 120
tgcatacttg attgggattt ctctgggctg gatcggttgg tagaggtcaa gaccatttag 180
tagcttagat atgtccgata gccgatacga tagcggatgc atcaacattg aagctatact 240
tcgagaatcg tggtgcttcc ttaggtccgt tatgcctgtc gaaaccattc ttgcttatca 300
gccctcgaga gccttggcct cgatcacttc tccttttacc tcgtatgggc cccaggtctg 360
ttacccctac gatctctatt atacggctgg tatcgatccc tgttcaacct cgattctcga 420
tcgatgtcac tcttggtaac ggatccagaa ggagccccca gctggtcgtc ttcgactcta 480
atcttcgatt gatatcgatt atgtatatcg gcccaggtaa cagccgggta ctcaatcaag 540
ttctgcttca actgttgtga agccactgaa cttcgttcat ttagtccttg ggtgaaagct 600
tgaacggccc aatcgtccgt gaccggtggt agattcattc gttccatttg gaaacgagat 660
acaaactctc taagcatctc gttatctacc tgctttacct tgaaaaggtc cgactttcta 720
gtctcgacct ttatggctac ggcgtgtgct tttacaaaag aatctgcaag catggcaaaa 780
gaatcgatag agttaggtgg taaattatga taccatatca ttgctccctt tgacagggtt 840
tccccgaatt tctttagtaa tacggatttg atctcgtcgt cctctagatc attcccttta 900
atggcatacg tgtaagaggt gacatgctcg ttggggtcga tcgttccatt atacttagga 960
atctcggtca tgcagaactt cttggagatc ggtttgggag ctgcgctgag ggggaagggc 1020
tttggtacga actttttgga atctaaaccc ttcaatattg gtggtgcccc ggggatcaga 1080
tcaaccctgg agttataagt gtccactttc ttgtcgtttg cttcgatcct cctttcccct 1140
gactctatcc attttgtcat ttcctcgagc atctttataa tttcggggtt agtccccgat 1200
tcttgtacat ttaaccttac cacaactggc cccgttctgt gggtgactta ttggggtgga 1260
tcgggctcaa ccctgctcgg cgcctgggta tggctttgta actgagctat cgccacctgt 1320
tgagcttgta gcatttcaaa tatcatacgc aagttgatcc cgtcttctcc aatattttgg 1380
atatttctaa ctgcagatcg agttccacca tgtatgctat tttcgggacc ggaatgttgg 1440
ttcgcttcga tggccacatg cgaattaacg tcaatcggat ccacggcttg agtcccaacg 1500
gggtcagcag gtggcctttc atccctgggc gtcacgttgt tattctcacc ttgatggcca 1560
aattcattat cgatatgcac ggggagtaat tgagagttcg tcatctttag cctgaaatca 1620
aagatacttc caagagcaag tgcaaaatat ggtgttttat agagatttgt atcaaataac 1680
cactattatc cttagcccca cagtgggcgc caaactgtta acccaaaaaa tggataacaa 1740
ttgaatttat aagcggttct aaggatacgt agtataactt ggtacaaatt gagaaaatat 1800
ataaatgaat atcgaaatta actgtaaaaa aaatgaatgc aaaccgaatg aattaattag 1860
cctaggccct caagtttgat caccctcaaa ctgaatggag agtagactga tacaagaaca 1920
aaatgattga atatcgaaac cagaaaaagg cagtatattg ctttatattc tatgaatctg 1980
aatctcctcc cttacaaata atcagacccc ctttatatag tagggaagtc ctattcttaa 2040
tataatttct aaataccgta aggaatccca tgatagatta attaattggc ctcttcttga 2100
tatgcgccgg gatttccgtt cagattttcg cccaattgcg gatattccgg ttttctattt 2160
tttggctcga taagctctcc tcaattttgg ctgacctcga tcttgatcgg tctctaactt 2220
gctcgatctc gatctcgatc ttggccgatc tcgatcgtga ccggtctcga tcttgctcga 2280
tctcaatctt ggccgatatc gatcttgatc ggtctctgga tcacgagctc ggtaacctaa 2340
ctttgcatta tggctcgatt ctacacgagg ccataccttg gtctatcata ttccaatctc 2400
gattaatcat acgaagggca aactcggttt tgaccgtata cagaaatgat gttctataat 2460
cgattacaat ttcaataaaa gaaaataatc ttatagtaat aaagtttata tataaatctc 2520
ctgtcttatt tttttaccga ttcttataga aagtgtcata tatagctcaa aaacatttca 2580
aatgagtctt aaaaaccaat ctcccggtat ccttgcctct tcctttttct tttggtttct 2640
cattcagtat aagtatcaat tttggtctat ctaatttgga ttcgtgctaa aaattcattg 2700
tcggttaaag tgctccttta atttgcactc catttgatat ggacactttc gttttcgact 2760
tttcacaatt atttgacaac actctgctaa tgagttataa agtgaagttc tctgtatgaa 2820
gaggatagat taagcttttc atatgtcaag tgaatattaa tttctggtaa aatttaaata 2880
ccatcgataa gaaaaatata aaatgcaatg tgaacagtaa ttttttggat ttggccccta 2940
gttaggcagt agcactttca tatgtagcag cttagaattc agtgttttct catgtccctt 3000
ccagtagtag tatgaaagga cacttaaact attcatttaa cctaatctca gtttaataat 3060
ggagtactca gtttcaattc tctttttttt tttccctttc tccattttag gtacatggga 3120
atattatcta attactgatc gacacatttt tgtatatctg tatatgtcga aattgacaaa 3180
aagataactt tgcatgttaa gttaattaaa atattgcatc taactaggtg gaaataatat 3240
ttttggcaat agtactttaa gtttatgtat taagaataca aaaactattt atatcaggag 3300
agtaaaaaag atatatgcat taattggtag agcttaacta gtatccatcg cctatagaga 3360
tcttttacac aatcaaatta tcattgtttg ttctaacaaa caacttattt aatttatttt 3420
caaaattata aatttcagtt ttaaagagaa ttacttgtaa ataatttctg aattacctga 3480
tggtgtaaaa tattaagcca tattatatat acaaagaaaa aaatcaggag atgaggcaaa 3540
acatccaaca atccttgcgt atacagaaat acttttacgt actgtcagta atattaggta 3600
attttcaatg gcagacctct tttgttgccc tattgaccct acaattggag gggtatttac 3660
ccccaagaaa ctcgatcgta atcttgccct aaagattggc tgactcaaat cagatgacca 3720
catttctata ttgtcccacg tacctaacgc aatcttcttc ctctatataa accatgcatg 3780
gacaatctcc tcttctcaaa cttcataaag atattatatt aaaaaaaata aagaagaaga 3840
gaagatagag gtaattagct atagcaatgg atcaacaaca ttccacttgt ttttcttctt 3900
caagcaaaat taatgacaaa gaaaagaaga aaaaaggatc agttgtgaaa ctatcaactg 3960
atccacaaag tgtagcagct cgtgaaagaa ggcatagaat cagtgatcgt ttcaagattt 4020
tgcagagttt agtccctggt ggttctaaaa tggacacagt tacaatgtta gaagaagcaa 4080
ttcactatgt caaatttctc aagatgcaaa tatggctgca tcaaaccatg attaatattg 4140
tagatgatta tgataatcca aattatcatc atcagttgct aatggctcat gactctaatt 4200
ttgctaatta ttatcctcat gagaataact caactcctgt tgagaatgca caaataaatt 4260
ataacttgga ccagctgcag cttccaggtt atgcattttc agatggagat caattccaag 4320
gagaagaaac taatatttct ggtgatgctt ttatgtacta ttaattagta attagttaat 4380
tatgttgcct aagtttaatt agaatacgta gtgtgtggta gtatggtatg ttgttttctc 4440
tctttctatc tagcagccta atgatgggtt tgtgttaatt aattagatgt agtaaattgt 4500
aagtgttggt tagttgatta agtatgttgc aagtttgtga agaagcatct ggaagttcaa 4560
tttgcctaat gtataacatt ttttaaataa aaactgaacg gacaaaaaga gtgaaagagg 4620
aaagaaagag agaaaattaa agaaaccaaa ttacgggagg tttagaaaca atttaagtct 4680
atactacctc ttattacctc ttattaatta taatataaag aatatttaca taattaaatc 4740
atttttatgg tatttaacgg tttagctatt atgtagtgaa acgtctatct tttctggtgg 4800
caacgtatca agtctgcttg gccaaacttt taaaaacggc tcaaattttg gaattacttt 4860
tgtcaaaata acttttcgtg gaggttcttc cggagagaat taaatgcata tttatttgtc 4920
atactgggat aatattcgaa ttaaatatcc agtgggccta aagctgctga tagaggcctg 4980
aaaacatggg cctaagtctg ctgaaagctc gttaaatacg ctaacctgaa atagtgcttc 5040
cttaaaaata agggattttg gcatggtgtg acaacttacc tacataattg gtagaaacaa 5100
atcctagtta taaatattgg caccagatag ccaataaata tatatcacaa aaagaatgtg 5160
tatatcacaa tatttttctt ctttgtgtat atcgaaatat atattaattt tttttctttg 5220
tatatctaaa atataaaatg ataaatatag ttgtttaaaa tttattgtta tctctatatc 5280
aatctatatc acaacaaaaa taaatgcatt gcttgtatat cacagtatat gacacatcaa 5340
atatgtgtat accaaaatgg acatcacaaa tttatgtttc ttctttgtgt atatcaaact 5400
gtatatcaaa aaattatttt ccttttttat atattaaaat ttatataaaa tttatttatt 5460
cctacaatta tatttattgt ttatatttta gaactttctc taaacctatt atatcgggga 5520
aaaaaattat ttgacaagaa caaaattgaa ataagtttat tcaaaataaa tttctccctc 5580
ttaaaattaa actggacgat ttgattccga tttaatatat tgccaaatct aacaatgtat 5640
attacgatat acaacatgat atacacacag gtatatatgg tgatagacgt agatgtacac 5700
cacctcaaac acacatattt ttatcttaca ataagaaaaa aagaaaaaaa aaggaagaag 5760
aagaaaattg gaatatgccg cttgaaaaaa gaaagagaag aacggcgaaa ttcattagta 5820
cgagaattta catataagat atgtaaatat cgaatgaaaa atatgatccg gcaatcgcaa 5880
tattggattc tcataataat tagtgcgaga aacttttgtg acatgcggca tacttcggcc 5940
atatcattgg aattgaaaca acacaaaaaa aaaaatcaaa aatccttacc ctaacttcgt 6000
ttgaggcgca gaccaagttc tacaaggaaa ctttataatt ctattttttt tcttttactt 6060
caagacctta aaattctatc aaaagtaatt ctcacacgca atattaaagg gtccaagtaa 6120
aaattattga aatacgggga aagaaaagat aaaaacattg aagaagaaat acgggggatt 6180
ttaggatttg gctatataat ggcaattctt ttaggctgcc tttgccgaat ttaatacaag 6240
gctaaaaaag tgtcaattct ctttatgggt tgtcatacca tgccattttc tctataaaga 6300
atttaggggt attattattt ttagcccccg tcagaaacta tttatattaa ttaggtagcc 6360
aaaaaagtat ataaattttt tataattttt atatataaca tacataatgt gtgtgtgtgt 6420
gtgtatatat atatatatat atatatatat atattcggct attattttga gagcggttat 6480
ataatgtcat tttcccaaaa tttgagatca tctaaattag gattgtgatt ttatggaatt 6540
ttatggtttc tctgcatgtc aagaacatat aggtaagaca catttttagg agatttcgga 6600
gataaactga acaataattt tttcttgtgt gtttgaaagt aaaagaaaag attacaataa 6660
gaagaaaaaa gattcatctt ttatttttat tatatgaacc gtaatatctt ataactaagg 6720
gaaaagggca aaatatactc ctcaactttc ggatattatc taaatttaat attcgttata 6780
ctatcatgtc aaatttatcc ctatcgttat attatccagc caaatttacc cctatcatca 6840
ccaaattttt aaaaattaac ccttgatctg ttaggttatc caaaatctcc caattttttt 6900
tttaatttaa attgcttgtt attcttcttg ctccactatt ttcagaaatt actattgaat 6960
gctatatgta ctagactata caaattattt atggatattt tttgattacc attgcaccca 7020
cttctcttct tgtgataatg ggtttggaat tggtttaaaa ttttatttat ttttcaacta 7080
tatacacact gtagaattca gttggtctat ttattgtgta ttatatgcag ctgctcatca 7140
tgtatataca tgcatatttg aatatatttt gttattgtct agttagtata gagttcgatc 7200
gactagctta agagtgcaca atccataggc atttgattaa agcaaagttg aattcgataa 7260
tgtaaattct ccaaagaact tcaacttcta tcactaatat ttgtaaagtc tccatacttt 7320
tggtgcagca aattcattaa agatagggtg ttgtaaatac ttttaaaatt tagataagct 7380
acttaatttc gattcttcaa tttactatat tttgtttact aaccgttgta ttattttaat 7440
atttcttttt cttatttttt ttaattaagg acgcaggtaa atgccaacta tttcgaaaat 7500
agtgaatcaa gaagaagaat gagtgactta attaaaataa tttggaagat tctgggtcac 7560
ctgacggatt gaggggtaac ttttaaaagt ttgttgatga taggggtaaa tttggctgga 7620
taatataacg ataaggataa atttgactga atagtataac aaaggttaaa tgtaaagaat 7680
attcgaaagt agagggataa atttggacat tttccctata actaaaaggt aatgactaat 7740
ggggaatgac ttagacttct caagtagctt ttggttattt tttacgttat aatatacttt 7800
tctaatgaca aaaaacctag gcatctagct ttcagcatgc atgcctacat attagactaa 7860
aatataaaat ttcaatacaa ctaagcttct ttatgtgtgg gatcttagac aaaaatctaa 7920
tcaaattaaa ttggccgaat agtctcgtag actcttgtat ttgtttcagt ttgtcatttc 7980
ggtccttgta atccacaaag tttcatccaa acacttatag ttatcaaaat atactacttt 8040
aaattcctct gacagttgac tgaacatatg tggcaacttg atggctgagg aggacaaaat 8100
atgtgtgttt cacgtgtata acaagcgtga gtatgaatat taaaacaaaa aataattaaa 8160
taagaaagaa tgttttaaaa taaaagaaaa agacgagaca aatagtaaga aaagaaaatt 8220
agacaaaacc caaaaatgaa aaactccagt tactcaatcc ctgtcgttct cctcctcatc 8280
gtccgccccc ctctctcaaa agtaaataaa ggataaagct tagatctaaa aacaccgata 8340
ttctgttgta aaaaaaaagt ttgttctttc ataaaactaa ataccgcaat tcaatcacaa 8400
ctgtttctcc caaaaccata tccaaatata catgcatgtt ttccattaat taaaccaaaa 8460
tgcaaaagta aaactgttcc aatctgtacg cttttagcat ctggaacttc gttttggcaa 8520
tcta 8524
<210> 64
<211> 181
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 64
Met Ala Met Val Val Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Tyr Arg Asn Cys Cys
180
<210> 65
<211> 837
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 65
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Thr Gly
165 170 175
Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly Pro Asp
180 185 190
Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val Ala Ala
195 200 205
Arg Ala Cys Gly Leu Val Ser Leu Glu Pro Thr Lys Ile Ala Glu Ile
210 215 220
Leu Lys Asp Arg Ser Ser Trp Phe Arg Asp Cys Arg Asn Val Glu Val
225 230 235 240
Phe Thr Met Phe Ser Ala Gly Asn Gly Thr Ile Glu Leu Leu Tyr Thr
245 250 255
Gln Ile Tyr Ala Pro Thr Thr Leu Ala Pro Ala Arg Asp Phe Trp Thr
260 265 270
Leu Arg Tyr Thr Thr Thr Leu Glu Asn Gly Ser Phe Val Val Cys Glu
275 280 285
Arg Ser Leu Ser Gly Thr Gly Ala Gly Pro Asn Ala Ala Ser Ala Ser
290 295 300
Gln Phe Val Arg Ala Gln Met Leu Pro Ser Gly Tyr Leu Ile Arg Pro
305 310 315 320
Cys Asp Gly Gly Gly Ser Ile Ile His Ile Val Asp His Leu Asn Leu
325 330 335
Glu Ala Trp Ser Ala Pro Glu Ile Leu Arg Pro Leu Tyr Glu Ser Ser
340 345 350
Lys Val Val Ala Gln Lys Met Thr Ile Ala Ala Leu Arg Tyr Ala Arg
355 360 365
Gln Ile Ala Gln Glu Thr Ser Gly Glu Val Val Tyr Gly Leu Gly Arg
370 375 380
Gln Pro Ala Val Leu Arg Thr Phe Ser Gln Arg Leu Ser Arg Gly Phe
385 390 395 400
Asn Asp Ala Ile Asn Gly Phe Ser Asp Asp Gly Trp Ser Leu Leu Ser
405 410 415
Ser Asp Gly Gly Glu Asp Val Ile Val Ala Val Asn Ser Arg Lys Asn
420 425 430
Ile Ala Thr Thr Ser Val Pro Leu Ser Pro Leu Gly Gly Ile Leu Cys
435 440 445
Ala Lys Ala Ser Met Leu Leu Gln Asn Val Pro Pro Ala Val Leu Val
450 455 460
Arg Phe Leu Arg Glu His Arg Ser Glu Trp Ala Asp Phe Asn Val Asp
465 470 475 480
Ala Tyr Val Ala Ser Ser Met Lys Ser Cys Ser Tyr Ala Tyr Pro Gly
485 490 495
Val Arg Pro Thr Arg Phe Thr Gly Ser Gln Ile Ile Met Pro Leu Gly
500 505 510
His Thr Ile Glu His Glu Glu Met Leu Glu Val Ile Arg Leu Glu Gly
515 520 525
His Ser Ile Gly Gln Glu Asp Ala Phe Met Pro Arg Asp Ile His Leu
530 535 540
Leu Gln Met Cys Ser Gly Thr Asp Glu Asn Ala Val Gly Ala Cys Ser
545 550 555 560
Glu Leu Val Phe Ala Ala Ile Asp Glu Met Phe Pro Asp Asp Ala Pro
565 570 575
Leu Leu Pro Ser Gly Phe Arg Ile Ile Pro Leu Glu Ser Lys Ser Ser
580 585 590
Asp Pro Gln Asp Thr Ser Asn Ala His Arg Thr Leu Asp Leu Ala Ser
595 600 605
Ser Leu Glu Val Gly Pro Ala Thr Asn Pro Ala Thr Gly Asp Val Val
610 615 620
Ser Gly Tyr Ser Ala Arg Ser Val Leu Thr Ile Ala Phe Gln Phe Pro
625 630 635 640
Phe Glu Asp Asn Leu Gln Asp Asn Val Ala Thr Met Ala Arg Gln Tyr
645 650 655
Val Arg Ser Val Val Ser Ser Val Gln Arg Val Ala Met Ala Ile Ser
660 665 670
Pro Ala Gly Val Asn Ser Thr Phe Gly Ser Lys Leu Ser Pro Gly Ser
675 680 685
Pro Glu Ala Val Thr Leu Ser His Trp Ile Cys Gln Ser Tyr Ser Tyr
690 695 700
His Met Gly Thr Glu Leu Leu Gln Ala Asp Ser Arg Gly Asp Glu Ser
705 710 715 720
Val Leu Lys Asn Leu Trp Gln His Gln Asp Ala Ile Leu Cys Cys Ser
725 730 735
Leu Lys Ser Leu Pro Val Phe Ile Phe Ala Asn Lys Ala Gly Leu Asp
740 745 750
Met Leu Glu Thr Thr Leu Val Ala Leu Gln Asp Ile Thr Leu Asp Arg
755 760 765
Ile Phe Asp Glu Ser Gly Arg Lys Val Leu Phe Ala Glu Phe Pro Lys
770 775 780
Ile Met Asp Gln Gly Phe Ala Tyr Leu Pro Gly Gly Ile Cys Met Ser
785 790 795 800
Ala Met Gly Arg His Ile Ser Tyr Glu Gln Ala Ile Ala Trp Lys Val
805 810 815
Phe Ala Ser Glu Glu Thr Ser Val His Cys Leu Ala Phe Ser Phe Ile
820 825 830
Asn Trp Ser Phe Val
835
<210> 66
<211> 181
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 66
Met Ala Met Val Val Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Tyr Arg Asn Cys Cys
180
<210> 67
<211> 204
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 67
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Tyr Arg Asn Cys Cys Leu Gly Pro Asp Ala Trp Asp Glu Ala Trp Ser
180 185 190
Gly Phe Ser Trp Asp Phe Cys His Leu Thr Gln Leu
195 200
<210> 68
<211> 216
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 68
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Ala Met Trp Ser Cys
210 215
<210> 69
<211> 219
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 69
Met Ala Met Val Val Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Ala Arg His Val Val Leu Leu Val
210 215
<210> 70
<211> 837
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 70
Met Ala Met Val Val Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Cys Gly Leu Val Ser Leu Glu Pro Thr Lys Ile Ala Glu
210 215 220
Ile Leu Lys Asp Arg Pro Ser Trp Phe Arg Asp Cys Arg Asn Val Glu
225 230 235 240
Val Phe Thr Met Phe Ser Ala Gly Asn Gly Thr Ile Glu Leu Leu Tyr
245 250 255
Thr Gln Ile Tyr Ala Pro Thr Thr Leu Ala Pro Ala Arg Asp Phe Trp
260 265 270
Thr Leu Arg Tyr Thr Thr Thr Leu Glu Asn Gly Ser Phe Val Val Cys
275 280 285
Glu Arg Ser Leu Ser Gly Thr Gly Ala Gly Pro Asn Ala Ala Ser Ala
290 295 300
Ser Gln Phe Val Arg Ala Gln Met Leu Pro Ser Gly Tyr Leu Ile Arg
305 310 315 320
Pro Cys Asp Gly Gly Gly Ser Ile Ile His Ile Val Asp His Leu Asn
325 330 335
Leu Glu Ala Trp Ser Ala Pro Glu Ile Leu Arg Pro Leu Tyr Glu Ser
340 345 350
Ser Lys Val Val Ala Gln Lys Met Thr Ile Ala Ala Leu Arg Tyr Ala
355 360 365
Arg Gln Ile Ala Gln Glu Thr Ser Gly Glu Val Val Tyr Gly Leu Gly
370 375 380
Arg Gln Pro Ala Val Leu Arg Thr Phe Ser Gln Arg Leu Ser Arg Gly
385 390 395 400
Phe Asn Asp Ala Ile Asn Gly Phe Ser Asp Asp Gly Trp Ser Leu Leu
405 410 415
Ser Ser Asp Gly Gly Glu Asp Val Ile Val Ala Val Asn Ser Arg Lys
420 425 430
Asn Ile Ala Thr Thr Ser Val Pro Leu Ser Pro Leu Gly Gly Ile Leu
435 440 445
Cys Ala Lys Ala Ser Met Leu Leu Gln Asn Val Pro Pro Val Val Leu
450 455 460
Val Arg Phe Leu Arg Glu His Arg Ser Glu Trp Ala Asp Phe Asn Val
465 470 475 480
Asp Ala Tyr Val Ala Ser Ser Met Lys Ser Cys Ser Tyr Ala Tyr Pro
485 490 495
Gly Met Arg Pro Thr Arg Phe Thr Gly Ser Gln Ile Ile Met Pro Leu
500 505 510
Gly His Thr Ile Glu His Glu Glu Met Leu Glu Val Ile Arg Leu Glu
515 520 525
Gly His Ser Ile Gly Gln Glu Asp Thr Phe Met Pro Arg Asp Val His
530 535 540
Leu Leu Gln Met Cys Ser Gly Thr Asp Glu Asn Ala Val Gly Ala Cys
545 550 555 560
Ser Glu Leu Val Phe Ala Ala Ile Asp Glu Met Phe Pro Asp Asp Ala
565 570 575
Pro Leu Leu Pro Ser Gly Phe Arg Ile Ile Pro Leu Glu Ser Lys Ser
580 585 590
Ser Asp Pro Gln Asp Thr Ser Asn Ala His Arg Thr Leu Asp Leu Ala
595 600 605
Ser Ser Leu Glu Val Gly Pro Ala Thr Asn Pro Ala Thr Gly Asp Val
610 615 620
Val Ser Gly Tyr Ser Ala Arg Ser Val Leu Thr Ile Ala Phe Gln Phe
625 630 635 640
Pro Phe Glu Asp Asn Leu Gln Asp Asn Val Ala Thr Met Ala Arg Gln
645 650 655
Tyr Val Arg Ser Val Val Ser Ser Val Gln Arg Val Ala Met Ala Ile
660 665 670
Ser Pro Ala Gly Val Asn Ser Thr Phe Gly Ser Lys Leu Ser Pro Gly
675 680 685
Ser Pro Glu Ala Val Thr Leu Ser His Trp Ile Cys Gln Ser Tyr Ser
690 695 700
Tyr His Met Gly Thr Glu Leu Leu Gln Thr Asp Ser Arg Gly Asp Glu
705 710 715 720
Ser Val Leu Lys Asn Leu Trp Gln His Gln Asp Ala Ile Leu Cys Cys
725 730 735
Ser Leu Lys Ser Leu Pro Val Phe Ile Phe Ala Asn Lys Ala Gly Leu
740 745 750
Asp Met Leu Glu Thr Thr Leu Val Ala Leu Gln Asp Ile Thr Leu Asp
755 760 765
Lys Ile Phe Asp Glu Ser Gly Arg Lys Val Leu Phe Ala Glu Phe Pro
770 775 780
Lys Ile Met Glu Gln Gly Phe Ala Tyr Leu Pro Gly Gly Ile Cys Met
785 790 795 800
Ser Ala Met Gly Arg His Ile Ser Tyr Glu Gln Ala Ile Ala Trp Lys
805 810 815
Val Phe Ala Ser Glu Glu Thr Val His Cys Leu Ala Phe Ser Phe Ile
820 825 830
Asn Trp Ser Phe Val
835
<210> 71
<211> 265
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 71
Met Ala Met Val Ala Gln Gln His Arg Glu Ser Ser Ser Gly Ser Ile
1 5 10 15
Thr Lys His Leu Asp Ser Ser Gly Lys Tyr Val Arg Tyr Thr Ala Glu
20 25 30
Gln Val Glu Ala Leu Glu Arg Val Tyr Ala Glu Cys Pro Lys Pro Ser
35 40 45
Ser Leu Arg Arg Gln Gln Leu Ile Arg Glu Cys Pro Ile Leu Ser Asn
50 55 60
Ile Glu Pro Lys Gln Ile Lys Val Trp Phe Gln Asn Arg Arg Cys Arg
65 70 75 80
Glu Lys Gln Arg Lys Glu Ser Ser Arg Leu Gln Thr Val Asn Arg Lys
85 90 95
Leu Ser Ala Met Asn Lys Leu Leu Met Glu Glu Asn Asp Arg Leu Gln
100 105 110
Lys Gln Val Ser Gln Leu Val Cys Glu Asn Gly Phe Met Arg Gln Gln
115 120 125
Leu His Thr Ala Ser Ala Ala Thr Thr Asp Val Ser Cys Glu Ser Val
130 135 140
Val Thr Thr Pro Gln His Ser Leu Arg Asp Ala Asn Asn Pro Ala Gly
145 150 155 160
Leu Leu Ser Ile Ala Glu Glu Thr Leu Ala Glu Phe Leu Ser Lys Ala
165 170 175
Thr Gly Thr Ala Val Asp Trp Val Pro Met Pro Gly Met Lys Pro Gly
180 185 190
Pro Asp Ser Val Gly Ile Phe Ala Ile Ser His Ser Cys Ser Gly Val
195 200 205
Ala Ala Arg Gln Arg Leu Leu Arg Ser Ser Lys Ile Asp Leu Leu Gly
210 215 220
Ser Glu Ile Ala Gly Thr Leu Lys Phe Ser Gln Cys Phe Leu Gln Glu
225 230 235 240
Met Glu Gln Leu Asn Phe Cys Thr Arg Arg Tyr Met Leu Leu Pro Pro
245 250 255
Trp Leu Leu His Val Ile Phe Gly Leu
260 265
<210> 72
<211> 258
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 72
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Pro Gln Ala Leu Glu Arg Pro Arg Lys Val Lys Asp Phe
245 250 255
Phe Ala
<210> 73
<211> 262
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 73
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Leu Thr Gln Ala Leu Lys Arg Pro Arg Asn
245 250 255
Val Lys Asp Phe Phe Ala
260
<210> 74
<211> 259
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 74
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Leu Thr Gln Ala Leu Glu Arg Pro Arg Lys Val Lys Asp
245 250 255
Phe Phe Ala
<210> 75
<211> 259
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 75
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Leu Ser Gln Ala Leu Glu Arg Pro Arg Lys Val Lys Asp
245 250 255
Phe Phe Ala
<210> 76
<211> 249
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 76
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Thr Gly Ser
245
<210> 77
<211> 457
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 77
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Lys Leu Arg Ile
245 250 255
Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val Thr Leu Ala
260 265 270
Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln Arg Phe Val
275 280 285
Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu Glu Ala Thr
290 295 300
Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln Val Trp Phe
305 310 315 320
Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp Lys Arg Arg
325 330 335
Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu Arg Ser Cys
340 345 350
Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser Gln Ala Lys
355 360 365
Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln Leu Ser Val
370 375 380
Ser Ser Thr Ser Asn Ser Phe Ser Trp Val Gly Lys Ile Asn Pro Phe
385 390 395 400
Phe Pro Tyr Leu Leu Gly Val Lys Phe Lys Thr Leu Lys Asn Lys Ile
405 410 415
Phe Ile Tyr Leu His Gly Glu Gly Gln Arg Gly Leu Gln Ser Gln Val
420 425 430
Leu Phe Phe Phe Phe Tyr Ile Tyr Ile Leu Phe Gly Phe Lys Val Ile
435 440 445
Gly Leu Met Asn Val Leu Ile Leu Thr
450 455
<210> 78
<211> 412
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 78
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Met
245 250 255
Leu Arg Ile Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val
260 265 270
Thr Leu Ala Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln
275 280 285
Arg Phe Val Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu
290 295 300
Glu Ala Thr Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln
305 310 315 320
Val Trp Phe Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp
325 330 335
Lys Arg Arg Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu
340 345 350
Arg Ser Cys Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser
355 360 365
Gln Ala Lys Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln
370 375 380
Leu Ser Val Ser Ser Thr Ser Asn Ser Phe Ser Trp Val Gly Lys Ile
385 390 395 400
Asn Pro Phe Phe Pro Tyr Leu Leu Gly Val Lys Leu
405 410
<210> 79
<211> 457
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 79
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Lys Leu Arg Ile
245 250 255
Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val Thr Leu Ala
260 265 270
Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln Arg Phe Val
275 280 285
Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu Glu Ala Thr
290 295 300
Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln Val Trp Phe
305 310 315 320
Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp Lys Arg Arg
325 330 335
Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu Arg Ser Cys
340 345 350
Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser Gln Ala Lys
355 360 365
Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln Leu Ser Val
370 375 380
Ser Ser Thr Ser Asn Ser Phe Ser Trp Val Gly Lys Ile Asn Pro Phe
385 390 395 400
Phe Pro Tyr Leu Leu Gly Val Lys Phe Lys Thr Leu Lys Asn Lys Ile
405 410 415
Phe Ile Tyr Leu His Gly Glu Gly Gln Arg Gly Leu Gln Ser Gln Val
420 425 430
Leu Phe Phe Phe Phe Tyr Ile Tyr Ile Leu Phe Gly Phe Lys Val Ile
435 440 445
Gly Leu Met Asn Val Leu Ile Leu Thr
450 455
<210> 80
<211> 412
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 80
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Met
245 250 255
Leu Arg Ile Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val
260 265 270
Thr Leu Ala Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln
275 280 285
Arg Phe Val Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu
290 295 300
Glu Ala Thr Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln
305 310 315 320
Val Trp Phe Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp
325 330 335
Lys Arg Arg Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu
340 345 350
Arg Ser Cys Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser
355 360 365
Gln Ala Lys Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln
370 375 380
Leu Ser Val Ser Ser Thr Ser Asn Ser Phe Ser Trp Val Gly Lys Ile
385 390 395 400
Asn Pro Phe Phe Pro Tyr Leu Leu Gly Val Lys Leu
405 410
<210> 81
<211> 412
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 81
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Thr
1 5 10 15
Asp Asp Gln Arg Arg Arg Phe Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Leu Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu Leu Gln Ser
100 105 110
Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg Phe Ser Gln
115 120 125
Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp Asn Gln Gln
130 135 140
Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val Gln Trp Pro
145 150 155 160
Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro Thr Leu Arg
165 170 175
Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg Thr Gly Asp
180 185 190
Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe Gln Phe His
195 200 205
Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp Pro Ser Ile
210 215 220
Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala Ile Asn Cys
225 230 235 240
Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Lys Leu Arg Ile
245 250 255
Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val Thr Leu Ala
260 265 270
Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln Arg Phe Val
275 280 285
Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu Glu Ala Thr
290 295 300
Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln Val Trp Phe
305 310 315 320
Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp Lys Arg Arg
325 330 335
Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu Arg Ser Cys
340 345 350
Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser Gln Ala Lys
355 360 365
Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln Leu Ser Val
370 375 380
Ser Ser Thr Ser Asn Ser Phe Leu Leu Gly Leu Ala Lys Ser Thr Pro
385 390 395 400
Phe Phe His Ile Phe Leu Ala Leu Asn Leu Lys Pro
405 410
<210> 82
<211> 411
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 82
Met Leu Gly Ser Phe Gly Ser Ser Ser Gln Ser His Asp Glu Glu Ala
1 5 10 15
Asp Asp Gln Arg Arg Arg Cys Ser Ser Thr Ser Pro Ala Ile Gln Ile
20 25 30
Arg Gln Leu Leu Ile Ser Cys Ala Glu Leu Ile Ser Arg Ser Asp Phe
35 40 45
Ser Ala Ala Asn Arg Leu Leu Thr Ile Leu Ser Thr Asn Ser Ser Pro
50 55 60
Phe Gly Asp Ser Thr Glu Arg Leu Val His Gln Phe Thr Arg Ala Leu
65 70 75 80
Ser Ile Arg Leu Asn Arg Tyr Ile Ser Ser Ala Thr Asn Phe Leu Thr
85 90 95
Pro Asn Ala Ser Ser Asn Val Val Glu Ser Ser Asn Asp Ser Ala Leu
100 105 110
Leu Gln Ser Ser Tyr Leu Ser Leu Asn Gln Val Thr Pro Phe Ile Arg
115 120 125
Phe Ser Gln Leu Thr Ala Asn Gln Ala Ile Leu Glu Ala Ile Asn Asp
130 135 140
Asn Gln Gln Ala Ile His Ile Val Asp Phe Asp Ile Asn His Gly Val
145 150 155 160
Gln Trp Pro Pro Leu Met Gln Ala Leu Ala Asp Arg Tyr Pro Pro Pro
165 170 175
Thr Leu Arg Ile Thr Gly Thr Gly Asn Asp Leu Asp Thr Leu Arg Arg
180 185 190
Thr Gly Asp Arg Leu Ala Lys Phe Ala His Ser Leu Gly Leu Arg Phe
195 200 205
Gln Phe His Pro Leu Leu Ile Thr Asn Asn Asn Asp Asn Asp His Asp
210 215 220
Pro Ser Ile Ile Ser Ser Ile Val Leu Leu Pro Asp Glu Thr Leu Ala
225 230 235 240
Ile Asn Cys Val Phe Tyr Leu His Arg Leu Leu Lys Asp Arg Glu Met
245 250 255
Leu Arg Ile Phe Leu His Arg Ile Lys Ser Met Asn Pro Lys Val Val
260 265 270
Thr Leu Ala Glu Arg Glu Ala Asn His Asn His Pro Leu Phe Leu Gln
275 280 285
Arg Phe Val Glu Ala Leu Asp Tyr Tyr Ala Ala Val Phe Asp Ser Leu
290 295 300
Glu Ala Thr Leu Pro Pro Ser Ser Arg Glu Arg Met Thr Val Glu Gln
305 310 315 320
Val Trp Phe Gly Arg Glu Ile Ile Asp Ile Val Ala Ala Glu Gly Asp
325 330 335
Lys Arg Arg Glu Arg His Glu Arg Phe Arg Ser Trp Glu Val Met Leu
340 345 350
Arg Ser Cys Gly Phe Ser Asn Val Ala Leu Ser Pro Phe Ala Leu Ser
355 360 365
Gln Ala Lys Leu Leu Leu Arg Leu His Tyr Pro Ser Glu Gly Tyr Gln
370 375 380
Leu Ser Val Ser Ser Thr Ser Asn Ser Phe Phe Leu Gly Leu Ala Lys
385 390 395 400
Ser Thr Pro Phe Phe His Ile Phe Leu Ala Leu
405 410
<210> 83
<211> 109
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 83
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Leu Glu Tyr
100 105
<210> 84
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 84
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 85
<211> 109
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 85
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Leu Glu Tyr
100 105
<210> 86
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 86
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 87
<211> 337
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 87
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro
145 150 155 160
Asn Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr
165 170 175
Asn Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala
180 185 190
Thr Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met
195 200 205
Phe Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met
210 215 220
Ser Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu
225 230 235 240
Gln Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln
245 250 255
Asn Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln
260 265 270
Lys Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly
290 295 300
Asn Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val
305 310 315 320
Phe Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Glu Met Phe Tyr
325 330 335
Tyr
<210> 88
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 88
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 89
<211> 337
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 89
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro
145 150 155 160
Asn Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr
165 170 175
Asn Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala
180 185 190
Thr Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met
195 200 205
Phe Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met
210 215 220
Ser Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu
225 230 235 240
Gln Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln
245 250 255
Asn Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln
260 265 270
Lys Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly
290 295 300
Asn Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val
305 310 315 320
Phe Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Glu Met Phe Tyr
325 330 335
Tyr
<210> 90
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 90
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 91
<211> 109
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 91
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Leu Glu Tyr
100 105
<210> 92
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 92
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 93
<211> 109
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 93
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Leu Glu Tyr
100 105
<210> 94
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 94
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 95
<211> 336
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 95
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Tyr Trp Asn Thr Lys Leu
100 105 110
Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys Ser
115 120 125
Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile Asn
130 135 140
Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro Asn
145 150 155 160
Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr Asn
165 170 175
Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala Thr
180 185 190
Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met Phe
195 200 205
Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met Ser
210 215 220
Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu Gln
225 230 235 240
Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln Asn
245 250 255
Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln Lys
260 265 270
Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr Asp
275 280 285
Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly Asn
290 295 300
Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val Phe
305 310 315 320
Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Glu Met Phe Tyr Tyr
325 330 335
<210> 96
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 96
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 97
<211> 337
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 97
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro
145 150 155 160
Asn Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr
165 170 175
Asn Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala
180 185 190
Thr Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met
195 200 205
Phe Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met
210 215 220
Ser Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu
225 230 235 240
Gln Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln
245 250 255
Asn Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln
260 265 270
Lys Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly
290 295 300
Asn Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val
305 310 315 320
Phe Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Glu Met Phe Tyr
325 330 335
Tyr
<210> 98
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 98
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 99
<211> 336
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 99
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Tyr Trp Asn Thr Lys Leu
100 105 110
Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys Ser
115 120 125
Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile Asn
130 135 140
Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro Asn
145 150 155 160
Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr Asn
165 170 175
Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala Thr
180 185 190
Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met Phe
195 200 205
Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met Ser
210 215 220
Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu Gln
225 230 235 240
Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln Asn
245 250 255
Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln Lys
260 265 270
Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr Asp
275 280 285
Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly Asn
290 295 300
Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val Phe
305 310 315 320
Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Glu Met Phe Tyr Tyr
325 330 335
<210> 100
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 100
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 101
<211> 336
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 101
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Tyr Trp Asn Thr Lys Leu
100 105 110
Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys Ser
115 120 125
Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile Asn
130 135 140
Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro Asn
145 150 155 160
Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr Asn
165 170 175
Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala Thr
180 185 190
Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met Phe
195 200 205
Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met Ser
210 215 220
Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu Gln
225 230 235 240
Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln Asn
245 250 255
Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln Lys
260 265 270
Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr Asp
275 280 285
Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly Asn
290 295 300
Asn Gly Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val Phe
305 310 315 320
Asn Asp Glu Asn Tyr Asn Lys Ser Asn Glu Ile Glu Met Phe Tyr Tyr
325 330 335
<210> 102
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 102
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 103
<211> 110
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 103
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Lys Leu Glu Tyr
100 105 110
<210> 104
<211> 112
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 104
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Glu Ile Leu Asn Ser Arg Lys Ser Leu Trp Asp
100 105 110
<210> 105
<211> 110
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 105
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Lys Leu Glu Tyr
100 105 110
<210> 106
<211> 119
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 106
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Thr Gly Ile Leu Asn
100 105 110
Ser Arg Lys Ser Leu Trp Asp
115
<210> 107
<211> 110
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 107
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Lys Leu Glu Tyr
100 105 110
<210> 108
<211> 112
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 108
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Glu Ile Leu Asn Ser Arg Lys Ser Leu Trp Asp
100 105 110
<210> 109
<211> 56
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 109
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu
50 55
<210> 110
<211> 64
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 110
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Lys Met Ala Lys Leu Ser Lys Ala
50 55 60
<210> 111
<211> 61
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 111
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Met Ala Lys Leu Phe Lys Ala
50 55 60
<210> 112
<211> 334
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 112
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Trp Leu Asn Tyr Leu Arg Pro Asn Ile
50 55 60
Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys Ser Leu
65 70 75 80
Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln Leu Pro
85 90 95
Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys Leu Lys
100 105 110
Lys Lys Leu Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys Ser Pro
115 120 125
Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile Asn Ser
130 135 140
Ser Leu Phe Arg Asp Leu Tyr Tyr Asn Pro Asn Asn Arg Pro Ile Ile
145 150 155 160
Thr Gly Leu Asn Gln Ser Ile Ser Ser Ala His Gln Pro Asn Phe Leu
165 170 175
Tyr Thr Asn Ser Asn Met Asn Phe Pro Asn Leu Gly Ala Thr Asn Ser
180 185 190
Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met Phe Gly Glu
195 200 205
Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met Ser Phe Gly
210 215 220
Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Cys Leu Gln Gln Gly
225 230 235 240
Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn Gln Asn Phe Thr Leu
245 250 255
Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln Lys Pro Asn Val
260 265 270
Tyr Phe Gly Asn Thr Thr Thr Thr Thr Gln Val Leu Gln Tyr Asp Val
275 280 285
Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Asn Gly Asn Asn Gly
290 295 300
Ser Thr Ile Gly Cys Asn Asn Asn Asn Ser Met Phe Val Phe Asn Asp
305 310 315 320
Glu Asn Tyr Asn Lys Ser Asn Glu Ile Gly Met Phe Tyr Tyr
325 330
<210> 113
<211> 56
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 113
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu
50 55
<210> 114
<211> 58
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 114
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Asp Gly
50 55
<210> 115
<211> 58
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 115
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ala Lys Leu Phe Lys Ala
50 55
<210> 116
<211> 55
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 116
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Ile
50 55
<210> 117
<211> 58
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 117
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ala Lys Leu Phe Lys Ala
50 55
<210> 118
<211> 55
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 118
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Ile
50 55
<210> 119
<211> 306
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 119
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro
145 150 155 160
Asn Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr
165 170 175
Asn Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala
180 185 190
Thr Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met
195 200 205
Phe Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met
210 215 220
Ser Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu
225 230 235 240
Gln Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln
245 250 255
Asn Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln
260 265 270
Lys Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Tyr Tyr
290 295 300
Trp Met
305
<210> 120
<211> 307
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 120
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Thr Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Pro Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Thr Pro Asn Asn Arg Pro
145 150 155 160
Asn Ile Thr Gly Leu Asn His Gln Ser Ile Ser Ser Ala His Gln Thr
165 170 175
Asn Phe Leu Tyr Thr Asn Asn Asn Met Asn Phe Pro Asn Leu Gly Ala
180 185 190
Thr Asn Asn Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met
195 200 205
Phe Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met
210 215 220
Ser Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Ser Leu
225 230 235 240
Gln Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn His Gln
245 250 255
Asn Phe Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln
260 265 270
Lys Pro Asn Val Tyr Phe Gly Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Asn Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Gln Trp
290 295 300
Gln Gln Trp
305
<210> 121
<211> 330
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 121
Met Gly Arg Ala Pro Cys Cys Asp Lys Ala Asn Val Lys Arg Gly Pro
1 5 10 15
Trp Ser Pro Glu Glu Asp Ala Lys Leu Lys Asp Phe Ile His Lys Tyr
20 25 30
Gly Thr Gly Gly Asn Trp Ile Ala Leu Pro Gln Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Leu Asn Tyr Leu Arg Pro
50 55 60
Asn Ile Lys His Gly Asp Phe Ser Glu Glu Glu Asp Arg Val Ile Cys
65 70 75 80
Ser Leu Tyr Ser Thr Ile Gly Ser Arg Trp Ser Ile Ile Ala Ala Gln
85 90 95
Leu Pro Gly Arg Thr Asp Asn Asp Ile Lys Asn Tyr Trp Asn Thr Lys
100 105 110
Leu Lys Lys Lys Leu Met Gly Leu Met Gln Ser Thr Asn Gln Arg Lys
115 120 125
Ser Pro Tyr Phe Pro Ala Thr Asn Ser Leu Gln Thr Gln Pro Gln Ile
130 135 140
Asn Ser Ser Leu Phe Arg Asp Leu Tyr Tyr Asn Pro Asn Asn Arg Pro
145 150 155 160
Ile Ile Thr Gly Leu Asn Gln Ser Ile Ser Ser Ala His Gln Pro Asn
165 170 175
Phe Leu Tyr Thr Asn Ser Asn Met Asn Phe Pro Asn Leu Gly Ala Thr
180 185 190
Asn Ser Gln Tyr Pro Tyr Asn Ile Gln Ser His Asn Leu Leu Met Phe
195 200 205
Gly Glu Ala Ser Cys Ser Ser Ser Asp Gly Ser Cys Ser Gln Met Ser
210 215 220
Phe Gly Lys Glu Ile Lys Arg Glu Glu Ile Met Ser Asn Cys Leu Gln
225 230 235 240
Gln Gly Gln Ile Ser Ser Val Asn Ala Phe Glu Glu Asn Gln Asn Phe
245 250 255
Thr Leu Asp Tyr Gly Asn Ser Ser Ser Asn Trp Val Asp Gln Lys Pro
260 265 270
Asn Val Tyr Phe Gly Asn Thr Thr Thr Thr Thr Gln Val Leu Gln Tyr
275 280 285
Asp Val Glu Glu Val Lys Gln Gln Leu Thr Ser Cys Thr Met Ala Thr
290 295 300
Met Ala Val Leu Leu Asp Val Thr Thr Thr Thr Val Cys Ser Cys Ser
305 310 315 320
Met Met Arg Ile Ile Thr Ser Gln Met Arg
325 330
<210> 122
<211> 54
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 122
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Arg Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Glu Gly Ile Glu Ser Val Ile Val
35 40 45
Ser Arg Phe Cys Arg Val
50
<210> 123
<211> 43
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 123
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Gly Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Lys Lys Ala
35 40
<210> 124
<211> 54
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 124
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Arg Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Glu Gly Ile Glu Ser Val Ile Val
35 40 45
Ser Arg Phe Cys Arg Val
50
<210> 125
<211> 43
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 125
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Gly Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Lys Lys Ala
35 40
<210> 126
<211> 43
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 126
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Arg Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Glu Lys Ala
35 40
<210> 127
<211> 39
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 127
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Gly Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Glu Asn Gln
35
<210> 128
<211> 43
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 128
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Arg Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Phe Leu Cys Gly
35 40
<210> 129
<211> 54
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 129
Met Asp Gln Gln His Ser Thr Cys Phe Ser Ser Ser Ser Lys Ile Asn
1 5 10 15
Asp Lys Glu Lys Lys Lys Lys Gly Ser Val Val Lys Leu Ser Thr Asp
20 25 30
Pro Gln Ser Val Ala Ala Arg Glu Glu Gly Ile Glu Ser Val Ile Val
35 40 45
Ser Arg Phe Cys Arg Val
50

Claims (21)

1.一种烟草植物体，其在基因组中导入了引起基因的功能抑制的突变，所述基因包含编码了以下(1)～(5)的多肽中任一者的多核苷酸作为编码区域，其中，
所述(1)～(5)的多肽为：
(1)相对于序列号1所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号3所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(2)相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(3)相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(4)相对于序列号13所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号15所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；以及
(5)相对于序列号17所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号19所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者，
所述功能抑制是抑制腋芽的发育。
2.根据权利要求1所述的烟草植物体，其中，所述功能抑制是抑制所述腋芽中在除去一级腋芽后发育的二级腋芽的发育。
3.根据权利要求2所述的烟草植物体，其中，所述功能抑制使所述二级腋芽的数量或重量与野生株植物相比减少至1/2以下。
4.根据权利要求1～3中任一项所述的烟草植物体，其中，所述功能抑制为与野生株植物相比时所述多肽的存在量减少。
5.根据权利要求4所述的烟草植物体，其中，所述功能抑制为与野生株植物相比时所述多肽的翻译量减少。
6.根据权利要求4所述的烟草植物体，其中，所述功能抑制为与野生株植物相比时来自所述基因的mRNA的转录量减少。
7.根据权利要求4所述的烟草植物体，其中，所述功能抑制为从上述基因转录而成的mRNA的分解的促进。
8.根据权利要求1～7中任一项所述的烟草植物体，其中，在所述基因中导入了所述突变。
9.根据权利要求8所述的烟草植物体，其中，通过自然突变、突变原处理、基因组编辑或基因敲除导入了所述突变。
10.根据权利要求7所述的烟草植物体，其中，所述突变为表达促进所述mRNA分解的因子的多核苷酸插入所述基因存在的区域以外。
11.根据权利要求10所述的烟草植物体，其中，所述因子为反义RNA分子、RNAi分子或共抑制分子。
12.根据权利要求1～11中任一项所述的烟草植物体，其属于红花烟草或黄花烟草。
13.一种烟草植物体的制备方法，该方法包括：
导入引起基因的功能抑制的突变的步骤，所述基因包含编码了以下(1)～(5)的多肽中任一者的多核苷酸作为编码区域，
其中，所述(1)～(5)的多肽为：
(1)相对于序列号1所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号3所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(2)相对于序列号5所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号7所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(3)相对于序列号9所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号11所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；
(4)相对于序列号13所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号15所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者；以及
(5)相对于序列号17所示的氨基酸序列具有90％以上的序列同一性的多肽、及相对于序列号19所示的氨基酸序列具有90％以上的序列同一性的多肽中的至少一者，
所述功能抑制是抑制腋芽的发育。
14.根据权利要求13所述的制备方法，其进一步包括从所述导入的步骤中生成的个体中挑选所述腋芽中在除去一级腋芽后发育的二级腋芽的发育受到抑制的个体的步骤。
15.根据权利要求14所述的制备方法，其中，在所述挑选的步骤中，挑选所述二级腋芽的数量或重量比野生株植物有所减少的个体。
16.根据权利要求13～15中任一项所述的制备方法，其中，所述导入的步骤包括将所述突变导入所述基因。
17.根据权利要求16所述的制备方法，其中，所述导入的步骤通过自然突变、突变原处理、基因组编辑或基因敲除来实施。
18.根据权利要求13～17中任一项所述的制备方法，其中，所述导入的步骤包括将表达促进从所述基因转录而成的mRNA的分解的因子的多核苷酸插入所述基因存在的区域以外。
19.根据权利要求18所述的制备方法，其中，所述因子为反义RNA分子、RNAi分子或共抑制分子。
20.一种权利要求1～12中任一项所述的烟草植物体、或者通过权利要求13～19中任一项所述的制备方法得到的烟草植物体后代或通过该烟草植物体的交配而得到的育种后代。
21.一种烟叶，其是从权利要求1～12中任一项所述的烟草植物体、通过权利要求13～19中任一项所述的制备方法得到的烟草植物体、或者权利要求20所述的后代或育种后代收获而得到的。
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