CN107223155A - 单倍体诱导系 - Google Patents

单倍体诱导系 Download PDF

Info

Publication number
CN107223155A
CN107223155A CN201580061712.7A CN201580061712A CN107223155A CN 107223155 A CN107223155 A CN 107223155A CN 201580061712 A CN201580061712 A CN 201580061712A CN 107223155 A CN107223155 A CN 107223155A
Authority
CN
China
Prior art keywords
plant
nucleic acid
sequence
haploid
albumen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580061712.7A
Other languages
English (en)
Inventor
C·博尔杜安
M·克洛伊贝尔-迈茨
M·尼森
M·奥祖诺娃
F·韦尔特迈尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KWS SAAT SE and Co KGaA
Original Assignee
KWS SAAT SE and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KWS SAAT SE and Co KGaA filed Critical KWS SAAT SE and Co KGaA
Publication of CN107223155A publication Critical patent/CN107223155A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

本发明涉及提供技术工具例如核酸，其在植物中转录后或翻译后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，还涉及用于产生和鉴定非转基因和转基因植物单倍体诱导系的方法和用途，以及涉及已有植物单倍体诱导系的改良。

Description

单倍体诱导系
发明领域
本发明涉及通过分子生物学方法和标记技术和遗传工程修饰植物的领域。其涉及提供技术工具例如核酸和载体，以及涉及方法和用途，用于产生和鉴定非转基因和转基因植物单倍体诱导系(haploid inducer)，以及改良已有的植物单倍体诱导系。
发明背景
一般而言，在杂交植物生产中，作为亲本的两个育种系相互杂交，由于已知的杂种优势效应，其后代部分地产生相对于亲本系而言强烈增加的产量。所述育种系可以通过多个自交步骤获得，然而其需要进行多代并因此涉及巨大的时间成本。现代植物育种许多年以前已经越来越多地转变为经由单倍体诱导以及随后的染色体加倍在短得多的时间量内生成育种系。对此的一技术需求是有用的单倍体诱导系统，其同时也提供足够的高效以经济上可用。
例如，对于玉米(Zea mays)，已知母体体内诱导系统，其中待诱导的植物用诱导系的花粉授粉。然后由此生成的后代的多达10％仅仅含有种子亲本的简单(单倍体)染色体组。现在可获得用于玉米杂交育种的少数几个这样的诱导系。然而，这些全部都归因于Coe,1959描述的单一系“Stock 6”。这样的已知的诱导系的一个实例是RWS( et al.,2005)系。在过去，在这些品系上进行了多项QTL研究以鉴别诱导系相关基因座。Deimling等在1997年已经鉴定到玉米种染色体1的主要QTL(bin 1.04)。Barret et al.2008更精确的定位于染色体1上66.96MB和68.11MB之间的范围，Prigge et al.2012更精确的定位于62.9MB和70.8MB之间的范围，和随后Dong et al.2013更精确的定位于68.18MB和68.43MB之间的范围，其根据公开注释含有三个基因。全部位置信息指的是B73参考基因组，AGPv02版本。Dong et al.2014实现5％的诱导率似乎已经证明该基因座其自身的功能性。然而，不能排除错误的精细定位，因为由于缺乏轮回亲本中侧翼标记的信息，不可能明确地对所述QTL进行划界。
此外，WO 2012/030893公开了玉米中染色体1上诱导系相关基因座，然而，其与前述基因座显著不同并且更具体地定位于端粒处。在所考虑的基因组区域中没有重叠。
总的而言，从“Stock 6”获得的玉米品系中体内单倍体诱导的分子和发育特异性机制至今很大程度是未知的。例如可以考虑，发生受精，但是随后其导致染色体消除，然后允许产生单倍体后代。例如，Ravi&Chan(2010)已经描述了在具有组蛋白CenH3的系统中的此类机制。然而，在另一方面，受精也可失败，在三倍体胚乳中出现单倍体卵细胞的发育。如果不了解来自“Stock 6”的诱导系基因型背后的母体体内单倍体诱导基因和所负责的基因的知识，该玉米诱导系的定向改良或所述诱导基因向非诱导系基因型的转移，或在玉米非诱导系中体内单倍体诱导能力的定向处理实际上是不可能的。
此外，对于一些栽培植物，根本没有已知的高效(且因此是经济的)可用的系统用于产生单倍体和双-单倍体植物，例如高粱、黑麦或向日葵。
有需要提供遗传元件如基因或调控元件，其在转基因和/或非转基因方法中可用以允许通过体内诱导的单倍体开发，或改善单倍体开发的效率。
发明概述
针对上文所述的现有技术的背景获得本发明，其中本发明的目的是提供工具和方法，其可用于产生体内单倍体诱导系和/或提供单倍体植物。
根据本发明，所述目的通过这样的核酸实现，所述核酸在植物中转录或表达后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力。根据本发明的所述核酸可以作为转基因使用。在另一方面，与本发明的所述核酸之一相同的植物基因组中或植物单倍体诱导系基因组中的内源DNA序列也可以被修饰，使得在所述内源DNA序列转录或表达后，单倍体诱导系的特性被介导或单倍体诱导系的诱导能力被增加。本发明的核酸优选是分离的核酸，其提取自其天然或原始环境(遗传背景)。核酸可以是双链或单链的，且可以是线性或环状的。其因此可以是基因组DNA、合成的DNA、cDNA或RNA类型(如lncRNA、siRNA或miRNA)，其中RNA中存在核碱基尿嘧啶代替核碱基胸腺嘧啶。
在本发明一优选实施方式中，本发明的核酸或所述核酸编码的RNA或所述核酸编码的蛋白或多肽，对植物中花粉管的生长、植物花粉的能量代谢和/或优选在生殖细胞(例如其发育成花粉)中的着丝粒的活性有影响。
本发明的核酸的特征可在于，所述核酸或所述核酸编码的RNA或所述核酸编码的蛋白或多肽，适于或可用于与野生型植物的花粉相比加速或促进花粉管生长(例如，在植物花粉中)，其中本发明的核酸或所述核酸编码的RNA或所述核酸编码的蛋白或多肽如下文所描述使用。例如，本发明的核酸编码蛋白，其在植物花粉的花粉管中参与大分子的运输或影响该运输。属于这些的是例如SNAREv蛋白，其例如介导例如在花粉管顶端的果胶类或磷脂类的运输(Kato et al.,2010)。此外，磷脂酶类的酶——特别是磷脂酶A2或patatin磷脂酶——能够促进花粉管的生长(Kim et al.,2011)，而肌醇聚磷酸酯-5-磷酸酶类如肌醇-1,4,5-三磷酸-5-磷酸酶，可以抑制花粉管生长(Wang et al.,2012)。本发明的核酸可以用作转基因，用于加速花粉管生长的目的，其中其然后在植物或其部分中例如通过过表达方法，与野生型植物或其相应部分相比，增加花粉管生长促进基因的表达率或增加正调节(激活)花粉管生长促进基因或负调节(抑制)花粉管生长抑制基因的RNA如lncRNA的转录率，和/或在植物或其部分中通过RNAi方法或miRNA方法(Fire et al.,1998)，与野生型植物或其相应部分相比，减少花粉管生长抑制基因的表达率。在另一方面，植物基因组中或植物单倍体诱导系基因组中与本发明的核酸相同的内源DNA序列，或所述内源DNA序列的调控序列也可以被修饰，例如，通过诱变或“基因组编辑”。与未诱变的野生型植物相比，该修饰可以增加或减少植物中所述内源DNA序列的转录或表达率，或所述内源DNA序列编码的蛋白或多肽的活性或稳定性。例如，相比于未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物，植物中内源花粉管生长促进基因的表达率或正调节(激活)花粉管生长促进基因或负调节(抑制)花粉管生长抑制基因的内源RNA如lncRNA的转录率可以因此被增加，或者相比于未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物，植物中内源花粉管生长抑制基因的表达率或负调节(抑制)花粉管生长促进基因或正调节(激活)花粉管生长抑制基因的内源RNA如lncRNA的转录率可以因此被减少。此外，相比于未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物，植物中所述内源DNA序列编码的花粉管生长促进蛋白或多肽的活性或稳定性可以被增加，或者相比于未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物，植物中所述内源DNA序列编码的花粉管生长抑制蛋白或多肽的活性或稳定性可以被减少。
在进一步的实例中，本发明的核酸可以表征为，通过使用所述核酸，或通过使用所述核酸编码的RNA，或通过使用所述核酸编码的蛋白或多肽，植物中花粉的能量代谢与野生型植物相比可以被负影响。例如，这可以通过磷酸甘油酸变位酶或线粒体转运蛋白或线粒体输入受体(mitochondrial import receptor)实现。出于该目的，本发明的核酸可以在过表达方法中、或在RNAi方法中、或在miRNA方法中用作转基因(Fire et al.,1998)。在另一方面，植物基因组中或植物单倍体诱导系基因组中与本发明的核酸相同的内源DNA序列，或所述内源DNA序列的调控序列也可以被修饰，例如，通过诱变或“基因组编辑”。与未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物相比，该修饰可以增加或减少植物中所述内源DNA序列的转录或表达率，或所述内源DNA序列编码的蛋白或多肽的活性或稳定性。
在另一实例中，本发明的核酸还可以表征为，通过使用所述核酸或使用所述核酸编码的RNA或使用所述核酸编码的蛋白或多肽，植物中的着丝粒的活性与野生型相比被修饰(尤其是在早期胚胎发生中和优选在所述植物中发育成例如花粉的生殖细胞中)，其导致例如所述诱导系基因组的消除。着丝粒的活性可以通过DNA的染色质修饰或在组蛋白水平修饰，此外，还可以通过转录、RNA相互作用或RNA结合。例如，着丝粒的活性的改变可以通过甲基转移酶如RNA甲基转移酶实现。出于该目的，本发明的核酸用作转基因，其中它然后通过过表达方法相对于野生型植物增加植物中染色质修饰基因或正调节(激活)染色质修饰基因的RNA(如lncRNA)的表达率。在另一方面，植物基因组中或植物单倍体诱导系基因组中与本发明的核酸相同的内源DNA序列，或所述内源DNA序列的调控序列也可以被修饰，例如，通过诱变或“基因组编辑”。与未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物相比，该修饰可以增加或减少植物中所述内源DNA序列的转录或表达率，或所述内源DNA序列编码的蛋白或多肽的活性或稳定性。与未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物相比，植物中染色质修饰基因或正调节(激活)染色质修饰基因的RNA(如lncRNA)的表达率因此也可以被增加。此外，与未诱变的野生型植物或未经由“基因组编辑”修饰的野生型植物相比，植物中所述内源DNA序列编码的染色质修饰蛋白的活性或稳定性可以被增加。
前面所述的本发明的核酸或所述核酸编码的RNA或所述核酸编码的蛋白或多肽的用途不是排他性的或限制性的，相反应该被理解为仅仅是示例。本领域技术人员从现有技术已知许多额外的技术手段和方法，通过所述技术手段和方法其可以实现上文所描述的本发明的核酸或相同的内源DNA序列的表达或转录率的改变，或上文所描述的有本发明的核酸或所述内源DNA序列编码的蛋白或多肽的稳定性和活性的改变。
在本发明一特别优选的实施方案中，所述核酸，其在植物中转录后或翻译后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，所述核酸可以包含这样的核苷酸序列，其
(i)选自SEQ ID No:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、42、43、46、47、49、50、52、53、55、56、57、58、59、60、61和/或62，或具有这些的功能性片段，或
(ii)与来自(i)的序列互补，或
(iii)与来自(i)的序列至少80％、82％、84％、86％、88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同，或
(iv)编码具有选自SEQ ID No:16、17、18、19、20、21、22、23、24、25、44、45、48、51、54、63、64和/或65的氨基酸序列的蛋白或所述蛋白的功能性部分，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其功能性部分，或
(vi)与来自(ii)的序列在严格条件下杂交。
该核酸可以编码蛋白或其功能性部分，其中所述蛋白或其功能性部分具有SNARE蛋白(尤其SNAREv蛋白)、磷脂酶(尤其磷脂酶A2或patatin磷脂酶)、甲基转移酶(尤其RNA甲基转移酶)或线粒体输入受体的功能(见表1)。可以如上所述实现所述核酸的用途，即为了在植物中介导单倍体诱导系的特性或增加单倍体诱导系的诱导能力，例如，所述核酸的表达率或所编码的蛋白或所编码的蛋白部分的活性或稳定性被转基因地或内源地增加。由于该核酸或该核酸编码的RNA或该核酸编码的蛋白或多肽对植物的单倍体诱导能力有正作用，在下文中，此处所定义的核酸命名为诱导促进核酸。下文进一步公开所述诱导促进核酸以及包含所述诱导促进核酸的物质的额外的方法和用途。
在本发明另一特别优选的实施方案中，所述核酸，其在植物中转录后或翻译后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，可以是包含这样的核苷酸序列的核酸，所述核苷酸序列
(i)具有选自SEQ ID No:26、27、28、29、30和/或31的序列或其功能性片段，或
(ii)与来自(i)的序列互补，或
(iii)与来自(i)的序列至少80％、82％、84％、86％、88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同，
(iv)编码具有选自SEQ ID No:32、33和/或34的氨基酸序列的蛋白或所述蛋白的功能性部分，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其功能性部分，或
(vi)与来自(ii)的序列在严格条件下杂交。
这样的核酸可以编码蛋白或其功能性部分，其中所述蛋白或其功能性部分具有肌醇聚磷酸酯-5-磷酸酶(特别是肌醇-1,4,5-三磷酸-5-磷酸酶)或磷酸甘油酸变位酶的功能(见表1)。可以如上所述实现所述核酸的用途，即为了在植物中介导单倍体诱导系的特性或增加单倍体诱导系的诱导能力，例如，所述核酸的表达率或所编码的蛋白或所编码的蛋白部分的活性或稳定性被转基因地或内源地减少。由于该核酸或该核酸编码的RNA或该核酸编码的蛋白或多肽对植物的单倍体诱导能力有负作用，在下文中，此处所定义的核酸命名为诱导抑制核酸。下文进一步公开所述诱导抑制核酸以及包含所述诱导抑制核酸的物质的额外的方法和用途。
在本发明另一特别优选的实施方案中，所述核酸，其在植物中转录后或翻译后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，其可以是这样的核酸，所述核酸编码具有双链部分的RNA，其中所述双链部分的至少一条链具有与以下核酸的编码序列中至少14、15、16、17、18、19、20、21、22、23、24、或25个，优选至少30、35、40、45、50、60、70、80、90、100、120、或140个，和特别优选至少160、180、200、250、300、350、400、450、500、600、700、800、900、或1000个连续核苷酸同源或相同的核苷酸序列：
(i)具有有义或反义方向的选自SEQ ID No:26、27、28、29、30和/或31的序列或其功能性片段的核酸，或
(ii)与来自(i)的序列互补的核酸，或
(iii)与来自(i)的序列至少80％、82％、84％、86％、或88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同的核酸，或
(iv)编码具有选自SEQ ID No:32、33和/或34的氨基酸序列的蛋白或所述蛋白的功能性部分的核酸，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其部分的核酸，或
(vi)与来自(ii)的序列在严格条件下杂交的核酸。在转录后基因沉默中，例如在RNAi方法和miRNA方法中所描述的(Fire et al.,1998)，这样的核酸可以用来抑制上文所述诱导抑制核酸的表达。dsRNA编码核酸也可以是编码长非编码RNA(lncRNA)的核酸。所述lncRNA核酸然后优选包含核苷酸序列，其
(a)具有选自SEQ ID No:35、36、37和/或38的序列或其片段，或
(b)与来自(a)的序列互补，或
(c)与来自(a)的序列至少80％、82％、84％、86％或88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同，或
(d)编码具有SEQ ID No:40或41的氨基酸序列的多肽或所述多肽的部分，或
(e)与来自(b)的序列在严格条件下杂交。该lncRNA，以下命名为lncRNA 1，可用于肌醇聚磷酸酯-5-磷酸酶例如肌醇-1,4,5-三磷酸-5-磷酸酶的表达或翻译调控。此外，所述lncRNA编码核酸可以优选包含核苷酸序列，其
(w)具有选自SEQ ID No:39的序列或其片段，或
(x)与来自(w)的序列互补，或
(y)与来自(w)的序列至少80％、82％、84％、86％或88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同，或
(z)与来自(x)的序列在严格条件下杂交。该lncRNA，以下命名为lncRNA 2，可用于磷脂酶尤其是磷脂酶A2或patatin磷脂酶的表达或翻译调控。
在本发明另一特别优选的实施方案中，所述核酸，其在植物中转录后或翻译后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，其可以是这样的核酸，所述核酸编码具有双链部分的RNA，其中所述双链部分的至少一条链具有与以下核酸的内含子序列中至少14、15、16、17、18、19、20、21、22、23、24、或25个，优选至少30、35、40、45、50、60、70、80、90、100、120、或140个，和特别优选至少160、180、200、250、300、350、400、450、500、600、700、800、900、或1000个连续核苷酸同源或相同的核苷酸序列：
(i)具有有义或反义方向的选自SEQ ID No:1、6、8、9、12、13、26、30、42、43、46、55、58和/或60的序列或其功能性片段的核酸，或
(ii)与来自(i)的序列互补的核酸，或
(iii)与来自(i)的序列至少80％、82％、84％、86％或88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同的核酸，或
(iv)编码具有选自SEQ ID No:16、17、18、19、20、21、22、23、24、25、44、45、48、63、64和/或65，或选自SEQ ID No:32、33和/或34的氨基酸序列的蛋白或所述蛋白的部分的核酸，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其部分的核酸，或
(vi)与来自(ii)的序列在严格条件下杂交的核酸。在转录基因沉默中，例如在RdDM方法中(Shibuya et al.,2009)，这样的核酸可以用来激活上文所述的诱导促进核酸的表达，或用于抑制上文所述的诱导抑制核酸的表达。dsRNA编码核酸也可以是编码长非编码RNA(lncRNA)的核酸。所述lncRNA核酸然后优选包含核苷酸序列，其
(a)具有选自SEQ ID No:35、36、37和/或38的序列或其片段，或
(b)与来自(a)的序列互补，或
(c)与来自(a)的序列至少80％、82％、84％、86％或88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同，或
(d)编码具有SEQ ID No:40或41的氨基酸序列的多肽或所述多肽的部分，或
(e)与来自(b)的序列在严格条件下杂交。该lncRNA，以下命名为lncRNA 1，可用于肌醇聚磷酸酯-5-磷酸酶例如肌醇-1,4,5-三磷酸-5-磷酸酶的表达或翻译调控。此外，所述lncRNA编码核酸可以优选包含核苷酸序列，其
(w)具有选自SEQ ID No:39的序列或其片段，或
(x)与来自(w)的序列互补，或
(y)与来自(w)的序列至少80％、82％、84％、86％或88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同，或
(z)与来自(x)的序列在严格条件下杂交。该lncRNA，以下命名为lncRNA 2，可用于磷脂酶尤其是磷脂酶A2或patatin磷脂酶的表达或翻译调控。
表1：序列表和核苷酸以及氨基酸序列的编号。基因家族/蛋白家族的名字对应于公开模型。诱导系中结构的变化可以导致不同的功能。
在另一方面，本发明涉及包含本发明的核酸的载体。所述载体可以是质粒、粘粒、噬菌体或表达载体、转化载体、穿梭载体或克隆载体；其可以是双链的或单链的，线性的或环状的；或其可以转化原核或真核宿主，通过整合进其基因组或在染色体外。本发明的核酸在载体中优选与一或多个调控序列可操作体连接，所述调控序列允许在原核或真核宿主细胞中的转录以及任选地允许表达。调控序列(优选DNA)可以与本发明的核酸同源或异源。例如，所述核酸在合适的启动子或终止子的控制下。合适的启动子可以是组成型诱导的启动子(实例：35S启动子，来自“花椰菜花叶病毒”(Odell et al.,1985)；那些组织特异性启动子是特别合适的(实例：花粉特异性启动子，Chen et al.(2010),Zhao et al.(2006),或Twell et al.(1991))，或者是发育特异性的(实例：开花特异性启动子)。合适的启动子也可以是合成的或嵌合的启动子，其在自然界中不存在，包含多个元件，且含有最小启动子，以及在所述最小启动子上游的至少一个顺式调控元件，其是特定转录因子的结合位点。嵌合启动子可以根据期望的特征设计，且由不同的因子诱导或抑制。这样的启动子的实例在Gurr&Rushton(2005)或Venter(2007)找到。例如，合适的终止子是nos终止子(Depicker etal.,1982)。
除了上文描述的载体外，本发明还提供一种方法，包括将所描述的载体插入进宿主细胞。例如，可以通过接合、转移(mobilization)、基因枪转化、农杆菌介导的转化、转染、转导、真空渗滤或电穿孔导入所述载体。这样的方法，如同制备所描述的载体的方法，是本领域技术人员的常识(Sambrook et al.,2001)。
在另一方面，本发明涉及包含本发明的核酸或本发明的载体的宿主细胞。本发明含义的宿主细胞可以是原核细胞(如细菌)或真核细胞(如植物细胞或酵母细胞)。所述宿主细胞优选是农杆菌，例如根癌农杆菌(Agrobacterium tumefaciens)或毛根农杆菌(Agrobacterium rhizogenes)，或植物细胞，其包含本发明的所述核酸或本发明的载体。对于本领域技术人员，已知可以用来将本发明的核酸或本发明的载体导入农杆菌的许多方法(如接合或电穿孔)，以及可以用来将本发明的核酸或本发明的载体导入植物细胞的方法如不同的转化方法(基因枪转化、农杆菌介导的转化)(Sambrook et al.,2001)。
在另一方面，本发明涉及转基因植物细胞，其包含作为转基因的本发明的核酸或包含本发明的载体，并涉及转基因植物或其部分，其包含所述转基因植物细胞。例如，这样的植物细胞或植物是用本发明的核酸或本发明的载体转化(优选稳定转化)的植物细胞或植物。本发明的转基因植物优选适于用作单倍体诱导系。在所述转基因植物的一优选实施方案中，所述核酸与一或多个调控序列可操作地连接，所述调控序列允许在植物细胞中的转录以及任选的表达。调控序列(优选DNA)可以与本发明的核酸同源或异源。由本发明的核酸和所述调控序列组成的总结构然后可以代表所述转基因。植物的部分可以是受精的或未受精的种子、胚、花粉、组织、器官、或植物细胞，其中所述受精的或未受精的种子、所述胚或所述花粉在所述转基因植物中产生，且本发明的核酸或所述载体整合进其基因组作为转基因。本发明而且还包括所述转基因植物的后代，其基因组中整合有本发明的核酸或载体作为转基因，且其适于用作单倍体诱导系。
在另一方面，本发明涉及由本发明的核酸编码的蛋白或多肽。所述蛋白或多肽优选适于在植物中介导单倍体诱导系的特性，或适于增加单倍体诱导系的诱导能力。特别优选由所述诱导促进核酸编码的蛋白或多肽。本发明的蛋白或多肽优选包括选自SEQ ID No:16、17、18、19、20、21、22、23、24、25、44、45、48、51、54、63、64和/或65，或选自SEQ ID No:32、33和/或34，或选自SEQ ID No:40和/或41的氨基酸序列。
在另一方面，本发明描述了用于产生适于用作单倍体诱导系的植物的方法。所述方法可以包括以下步骤：
A)诱变植物细胞且随后从所诱变的植物细胞再生植物或诱变植物，和
B)鉴定植物A)，其在与本发明的核酸相同的内源DNA序列中具有至少一个突变，或在所述内源DNA序列的调控序列(例如，启动子、增强子、终止子或内含子)中具有至少一个突变，所述突变导致与未诱变野生型植物相比所述内源DNA序列在所鉴定的植物中转录或表达率的变化，或者与未诱变野生型植物相比所述内源DNA序列编码的蛋白或多肽在所鉴定的植物中活性或稳定性的变化，其中所述至少一个突变在所鉴定的植物中导致要介导的单倍体诱导系特性或要增加的单倍体诱导系的诱导能力。所述转录率或表达率的改变，或所述活性或稳定性的改变，优选地至少在所鉴定的植物的花粉中或在所鉴定的植物的花粉组织中出现。
来自步骤B)的内源DNA序列，或所述内源DNA序列编码的RNA或所述DNA序列编码的蛋白或多肽，优选对植物中花粉管的生长、植物花粉的能量代谢和/或优选在生殖细胞(例如其发育成花粉)中的着丝粒的活性有影响。
所述用于产生适于用作单倍体诱导系的植物的方法的步骤B)的内源DNA序列特别优选地编码SNAREv蛋白；磷脂酶类的酶，特别是磷脂酶A2或patatin磷脂酶；肌醇聚磷酸酯-5-磷酸酶类的酶，例如肌醇-1,4,5-三磷酸-5-磷酸酶；磷酸甘油酸变位酶或甲基转移酶，特别是RNA甲基转移酶，其中，在SNARE蛋白、磷脂酶和甲基转移酶的情况下，所述转录率或表达率或所述活性或稳定性优选被改变至其被增加的程度，且其中，在肌醇聚磷酸酯-5-磷酸酶和磷酸甘油酸变位酶的情况下，所述转录率或表达率或所述活性或稳定性优选被改变至其被减少的程度。
所述用于产生植物的方法的步骤B)非常优选地是从A)鉴定植物，其a)在与所述诱导促进核酸或编码所述lncRNA 1的核酸相同的内源DNA序列中，或在所述内源DNA序列的调控序列(如启动子、增强子、终止子或内含子)中具有至少一个突变，其中所述至少一个突变实现所述内源DNA序列的转录或表达的增加或由所述内源DNA序列编码的蛋白或多肽的活性或稳定性的增加；和/或b)在与所述诱导抑制核酸或编码所述lncRNA 2的核酸相同的内源DNA序列中，或在所述内源DNA序列的调控序列(如启动子、增强子、终止子或内含子)中具有至少一个突变，其中所述至少一个突变实现所述内源DNA序列的转录或表达的减少或由所述内源DNA序列编码的蛋白或多肽的活性或稳定性的减少，其中a)和b)的所述至少一个突变在所鉴定的植物中导致要介导的单倍体诱导系特性或要增加的单倍体诱导系的诱导能力。所述转录率或表达率的改变，或所述活性或稳定性的改变，优选地至少在所鉴定的植物的花粉中或在所鉴定的植物的花粉组织中出现。
突变意指DNA水平的修饰，并因此是遗传学和/或表观遗传学的改变。例如，遗传学的改变可以是所述内源DNA序列中或所述内源DNA序列的调控序列中的至少一个核碱基的取代。如果这样的核碱基取代发生在例如启动子，这可以导致所述启动子改变的活性，由于例如顺式调控元件被修饰使得转录因子与所突变的顺式调控元件的亲和力相对于野生型启动子被改变，由此具有所述突变的顺数调控元件的启动子的活性被增加或减少，取决于所述转录因子是阻遏物还是诱导系，或所述转录因子与突变的顺式调控元件的亲和力被增强还是被弱化。如果这样的核碱基取代在例如所述内源DNA序列的编码区中发生，这可以导致所编码的蛋白的氨基酸取代，其可以导致所述蛋白的活性或稳定性与野生型蛋白相比的改变。遗传学改变的另一实例是在所述调控序列和/或所述内源DNA序列中的核苷酸缺失，以及在所述调控序列和/或所述内源DNA序列中的核苷酸添加。Das&Martienssen(1995)显示在玉米中通过转座子诱变插入核苷酸来调控基因。表观遗传学的改变可以通过DNA改变的甲基化模式发生。
本领域技术人员已知本发明含义中的突变如何能够通过用于产生适于用作单倍体诱导系的植物的方法的步骤A)的诱变过程实现。此处所述诱变包括常规诱变以及位点特异性诱变或“基因组编辑”。在常规诱变中，DNA水平的修饰不是以靶向的方式产生的。植物细胞或植物被暴露至诱变条件如TILLING，通过UV光暴露或使用化学物质(Till et al.,2004)。随机诱变的另外的方法是借助转座子的诱变。UniformMU项目制备了大容量的可免费获得的突变体库。所述库和所述方法描述于McCarty et al.(2005)。位点特异性诱变使得可以以靶导向方式在DNA中的预定位置在DNA水平导入修饰。例如，TALENS(WO 2010/079430,WO 2011/072246)，大范围核酸酶(Silva et al.,2011)，归巢内切核酸酶(Chevalier 2002)，锌指核酸酶(Lloyd et al.,2005)，或CRISPR/Cas系统(Gaj et al.,2013)可用于此。
步骤B)中植物的鉴定可以借助例如分子标记或探针实现。例如，DNA探针是引物或引物对，其可用于PCR反应。例如，可以通过在Tilling群体中测序靶基因来验证或鉴别Tilling突变体，或者通过额外的验证DNA中错配的方法，例如熔解点分析或使用错配特异性核酸酶。对此，本发明同样包括可用于此的引物/引物对，例如，针对磷脂酶、磷酸甘油酸变位酶、甲基转移酶和磷脂酶的lncRNA的引物。通过转座子产生的突变体也可以通过在跨越整个群体的PCR中使用转座子特异性引物和靶基因特异性引物以及随后测序PCR产物来验证。本发明也涵盖这样的引物。例如，花粉中表达率的改变可以用RT-PCR确定；稳定性的改变例如可以通过检查泛素结合位点和预测三级结构的变化来确定。此外，野生型蛋白以及相应突变体蛋白的重组表达，和随后的生化活性测试也是合适的。可用于在步骤B)中鉴别植物的额外的手段和方法是本领域技术人员从现有技术中知晓的。
本发明还涉及分子标记，其证明所述内源DNA序列中或所述内源DNA序列的内源DNA序列的突变的存在或不存在。例如，这样的标记基于SNP且对所述突变特异(实例：KASPar或TaqMan标记)。
本发明还进一步涉及能够用前述方法产生或用前述方法产生的植物，或该植物的部分，其中所述植物的部分可以是受精的或未受精的种子、胚、花粉、组织、器官或植物细胞，其中所述受精的或未受精的种子、所述胚、或所述花粉在所述转基因植物产生，且所述至少一个突变存在于其基因组中。本发明同样还包括所述植物的后代，其具有所述至少一个突变且适于用作单倍体诱导系。已经用前述方法产生的植物的两个实例是这样的植物(优选玉米或向日葵)，其在内源DNA序列中，具有以下核酸，所述核酸(i)具有选自SEQ IDNo:8、9和/或46的序列或其功能性片段；或(ii)与来自(i)的序列互补；或(iii)与来自(i)的序列至少80％相同；或(iv)编码具有选自SEQ ID No:21、22、23和/或48的氨基酸序列的蛋白或所述蛋白的功能性部分；或(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其功能性部分；或(vi)与来自(ii)的序列在严格条件下杂交，或在所述内源DNA序列的调控序列中具有至少一个突变，所述突变导致与未诱变野生型植物相比所述内源DNA序列在所鉴定的植物中转录或表达率的变化，或者与未诱变野生型植物相比所述内源DNA序列编码的蛋白或多肽在所鉴定的植物中活性或稳定性的变化，其中所述至少一个突变在所鉴定的植物中导致要介导的单倍体诱导系特性或要增加的单倍体诱导系的诱导能力。所述突变优选地是在SEQ ID No:8或9的编码序列中的改变(例如，点突变)，其导致SEQ ID No:21、22或23中氨基酸位置74-78之间的氨基酸取代，或者所述突变导致SEQ ID No.46的编码序列中的修饰，其导致SEQ ID No:48相应编码序列中的氨基酸取代。这可以包含根据SEQ IDNO:49-54的突变。通过TILLING导致的在SEQ ID NO:49中的突变导致第74位处的编码的氨基酸的氨基酸取代,其中天冬氨酸被天冬酰胺取代(D74N)；在SEQ ID NO:52中的突变导致第78位处的编码的氨基酸的氨基酸取代，其中甘氨酸被精氨酸取代(G74R)。
此外，本发明还涉及一种分离在植物中介导单倍体诱导系特性或增加单倍体诱导系的诱导能力的核酸的方法，包括以下步骤：
A)根据本发明前面描述的方法产生植物，或提供能够用本发明前述的方法产生或用本发明前述的方法产生的植物；和B)从来自A)的植物的基因组分离核酸，所述核酸包含具有所述至少一个突变的内源DNA序列。步骤B)中所述核酸非分离可以通过CTAB提取或通过DNA结合柱实现；所述突变的验证可以通过测序或分子标记如基于SNP的KASPar或TaqMan标记实现，或例如对于插入或缺失突变，通过基于长度多态性的标记实现。
本发明还包括通过或可通过前述用于分离的方法获得的核酸，以及包含所述分离的核酸的载体。
在另一方面，本发明还涉及用于产生适于用作单倍体诱导系的转基因植物的方法。所述方法可以包括以下步骤：
A)提供上文所述的核酸，其在植物中转录或表达后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力；或提供上文所述的分离的核酸，所述核酸包含所述具有至少一个突变的内源DNA序列；或提供上述的载体之一，
B)通过导入来自A)的核酸或载体转化(优选稳定转化)植物细胞，
C)从来自B)的经转化的植物细胞再生转基因植物，和
D)通过优选地在所鉴定的植物的花粉中的或在所鉴定的植物的花粉组织中的改变的表达模式从C)鉴定转基因植物，其中单倍体诱导系的特性被介导或单倍体诱导系的诱导能力被增加。所述用于产生适于用作单倍体诱导系的转基因植物的方法还包括提供两种或更多种上文所述的核酸(或者本发明的核酸的不同的实施方式，且任选地在一或多种载体中)以及通过导入两种或多种核酸转化植物细胞。可选地或额外地，除了本发明的核酸之外，还可以提供或转化已知可用于产生单倍体诱导系的一或多种额外的核酸(例如，经操作的cenh3基因(Ravi&Chan,2010))。
所述表达模式优选地被改变为实现
(I)与野生型植物(例如其再生自等基因未转化的植物细胞)相比，所鉴定的植物中所导入的诱导促进核酸或导入的编码lncRNA 1的核酸的转录或表达被增加，和/或
(II)与野生型植物(例如其再生自等基因未转化的植物细胞)相比，所鉴定的植物中所导入的诱导抑制核酸或导入的编码lncRNA 2的核酸的转录或表达被减少，和/或
(III)由于转录后基因沉默，与野生型植物(其例如再生自等基因、未转化的植物细胞)相比，在所鉴定的植物中具有与所述诱导抑制核酸相同的核苷酸序列的内源DNA序列的表达率通过所导入的核酸编码的双链RNA被降低，所述导入的核酸如上所述与转录后基因沉默相关，和/或
(IV)由于转录基因沉默，与野生型植物(其例如再生自等基因、未转化的植物细胞)相比，在所鉴定的植物中具有与所述诱导促进核酸相同的核苷酸序列的内源DNA序列或编码lncRNA 1的导入的核酸的转录率或表达率通过所导入的核酸(其在上文详细描述，与转录基因沉默相关)编码的双链RNA被增加；和/或与野生型植物(其例如再生自等基因、未转化的植物细胞)相比，在所鉴定的植物中具有与所述诱导抑制核酸相同的核苷酸序列的内源DNA序列或编码lncRNA 2的导入的核酸的转录率或表达率通过所导入的核酸(其在上文详细描述，与转录基因沉默相关)编码的双链RNA被减少。转录率的验证可以通过例如qRT-PCR实现。改变的蛋白稳定性可以通过例如蛋白印迹确定。
本发明还进一步涉及能够用前述方法产生或用前述方法产生的转基因植物或该植物的部分，植物的部分可以是受精的或未受精的种子、胚、花粉、组织、器官、或植物细胞，其中所述受精的或未受精的种子、所述胚或所述花粉在所述转基因植物中产生，且本发明的核酸或所述载体整合进其基因组作为转基因。本发明同样还包括所述转基因植物的后代，其具所导入的核酸作为转基因且适于用作单倍体诱导系。
在另一方面，本发明还涉及用于产生单倍体植物的方法，所述方法包括以下步骤：
A)是本发明的适于用作单倍体诱导系的非转基因或转基因植物和相同属优选相同物种的植物杂交，
B)选择受精的单倍体种子或胚，和
C)从来自B)的种子或胚再生单倍体植物。
所述适于用作单倍体诱导系的植物优选用作花粉亲本且和相同属优选相同物种的种子亲本杂交。所述适于用作单倍体诱导系的植物还可以用作种子亲本且和相同属优选相同物种的花粉亲本杂交。因此，步骤A)中的两种杂交配对物，种子亲本和花粉亲本，还可以是相同个体。所述杂交步骤因此代表自交。
所述单倍体受精的种子或胚的选择可以包括验证所述单倍性的步骤，已将所述单倍体受精的种子或胚与多倍体受精的种子或胚分开的步骤。所述受精的种子或胚的单倍性鉴定可以通过表型或基因型实现，例如，其中所述诱导系具有胚特异性可视标记，其在全部二倍体后代是可见的，但在诱导的单倍体后代则不可见。此外，所述倍性状态可以通过流式细胞术确定。此外，分子标记完整的、纯合的模式为单倍体植物提供指征。例如，所述分开可以自动地基于单倍性验证的数据实现。
本发明还进一步涉及单倍体受精的种子或胚，其通过所述用于产生单倍体植物的方法的步骤A)中的杂交而产生，以及涉及单倍体植物，其能够用所述方法或用所述方法产生，或者该植物的部分，其中植物的部分可以是种子、胚、组织、器官或植物细胞。本发明同样还包括所述植物的后代。此外，本发明还包括双-单倍体(二倍体)植物或其部分，其中所述双-单倍体(二倍体)植物或其部分通过所述单倍体植物或其部分的染色体加倍而产生。
在另一方面，本发明涉及本发明的核酸或本发明载体在植物中介导单倍体诱导系或增加单倍体诱导的诱导能力的用途，或本发明的核酸或本发明的载体用于产生适于用作单倍体诱导系的植物或转基因植物的用途。此外，本发明还包括本发明如上所述的植物的用途，其适于用作单倍体诱导系以产生单倍体的受精的种子或胚，或单倍体植物。之前针对本发明的主题和方法的解释也适用于所记载的用途。
在另一方面，本发明还涉及用于外部施加至植物的工具。提供该工具用于外部施加至植物且适于在植物中介导单倍体诱导系的特性，或适于增加单倍体诱导系植物的诱导能力。施加优选在花药形成、花粉形成或受精的时间点进行。所述工具包含具有双链部分的RNA，其中所述双链部分的至少一条链具有与以下核酸的编码序列中至少14、15、16、17、18、19、20、21、22、23、24或25个，优选至少30、35、40、45、50、60、70、80、90、100、120或140个，和特别优选至少160、180、200、250、300、350、400、450、500、600、700、800、900或1000个连续核苷酸同源或相同的核苷酸序列
(i)具有有义或反义方向的选自SEQ ID No:26、27、28、29、30和/或31的序列或其功能性片段的核酸，或
(ii)与来自(i)的序列互补的核酸，或
(iii)与来自(i)的序列至少80％、82％、84％、86％或88％，优选地至少90％、91％、92％、93％、94％、95％、96％，或特别优选地至少97％、97.5％、98％、98.5％、99％或99.5％相同的核酸，或
(iv)编码具有选自SEQ ID No:32、33和/或34的氨基酸序列的蛋白或所述蛋白的功能性部分的核酸，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其部分的核酸，或
(vi)与来自(ii)的序列在严格条件下杂交的核酸。
用于产生本发明所述工具的双链RNA可以通过本领域技术人员已知的方法体外产生。例如，可以合成产生所述双链RNA，其中所述RNA直接在体外形成。从双链DNA出发，所述双链RNA还可以通过例如形成mRNA转录物而合成，所述mRNA转录物然后形成发夹结构。所述工具可以用作在植物中诱导单倍体的触发器。例如，在植物开花前或后，可以通过以喷雾形式喷洒至植物组织上，或者通过本领域技术人员公知的额外的方式外部施加至植物组织上，或者通过喷雾或和其它添加剂混合来使用所述工具。例如，添加剂可以是润湿剂、载体物质或RNA稳定剂如脂质体。
令人惊奇地，本发明人已经发现，影响花粉管生长、花粉的能量代谢和/或着丝粒活性(优选在发育成例如花粉的生殖细胞中)的基因或基因产物特别适于将非单倍体诱导系转换成单倍体诱导系。为此，可以鉴定具有显著重要性的多个基因家族/蛋白家族。它们用于产生单倍体诱导系的用途之前在现有技术中没有被描述或提示。因为花粉的产生以及受精过程(包括花粉管的生长)在单子叶和双子叶植物中遵循广泛适用的原理，在本发明的技术教导下，即使是对之前既不存在高效体内单倍体诱导系统或其它用于产生双-单倍体植物的基于细胞培养的方法的栽培植物，本领域技术人员也接受开发单倍体诱导系的可能性。为此，使用他从本发明获得的遗传学信息，他可以通过常规劳动发现所描述的基因产物的同源物、直系同源物或类似物，并如本文所描述对它们进行操作。然而，本发明的技术教导也适于进一步针对它们的效率(即单倍体诱导率)改良已经存在的诱导系，并因此第一次使得它们能够经济地应用。此外，本领域技术人员将该技术教导和单倍体诱导的其它已知机制组合，例如操作CENH3蛋白(Ravi&Chan,2010)，并因此进一步增加效率。
本申请中使用的一些术语在下文详细解释：
“B73”是玉米育种系，其在玉米遗传学中用作模型基因型且被用于产生第一个玉米参考序列。
“介导单倍体诱导系的特性”或“单倍体诱导系的特性的介导”或相当的短语意指，通过使用本发明的核酸，使得植物在与来自相同属(优选相同物种)的不具有单倍体诱导系特性的植物杂交时能够产生具有单一染色体组(单倍体)的受精的种子或胚。单倍体诱导系的特性，定义为绝对单倍体诱导率，指的是至少0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、或1％，优选至少1.5％、2％、2.5％、3％、3.5％、4％、4.5％、或5％，或特别优选6％、7％、8％、9％、10％、11％、12％、13％、14％、或15％，或非常特别优选，至少20％、25％、30％、35％、40％、45％、或50％的所述受精的种子或胚具有单倍体染色体组。
“表达率的增加”或“增加的表达率”或“表达的激活”或相当的表述意指核苷酸序列的表达率与指定的参照相比增加超过10％、15％、20％、25％、或30％，优选增加超过40％、50％、60％、70％、80％、90％、或100％，或特别优选增加超过150％、200％、250％、300％、500％、或1000％。所述表达率的增加优选地导致其中表达率被增加的植物的表型变化。改变的表型可以是介导单倍体诱导系的特性，或单倍体诱导系诱导能力的增加。
“转录率的增加”或“增加的转录率”或相当的表述意指核苷酸序列的转录率与指定的参照相比增加超过10％、15％、20％、25％、或30％，优选增加超过40％、50％、60％、70％、80％、90％、或100％，或特别优选增加超过150％、200％、250％、300％、500％、或1000％。所述转录率的增加优选地导致其中转录率被增加的植物的表型变化。改变的表型可以是介导单倍体诱导系的特性，或单倍体诱导系诱导能力的增加。
核苷酸序列的“功能性”片段意指核苷酸序列的节段，其具有与所述功能性片段所源自的完整核苷酸序列相同或相当的功能。因此，所述功能性片段可以具有与所述完整核苷酸序列在至少50％、55％、60％、65％、70％、75％、80％、85％、90％、92％、94％96％、97％、98％、或99％的长度上相同或同源的核苷酸序列。此外，核苷酸序列的“功能性片段”还可以指核苷酸序列的节段，其改变总核苷酸序列的功能，例如在转录后或转录基因沉默中。因此，核苷酸序列的功能性片段可以包括完整核苷酸序列的至少14、15、16、17、18、19、20、21、22、23、24、或25个，优选至少30、35、40、45、50、60、70、80、90、100、120、或140个，或特别优选至少160、180、200、250、300、350、400、450、500、600、700、800、900、或1000个连续的核苷酸。
蛋白的“功能性部分”意指蛋白的节段，或编码所述蛋白的氨基酸序列的区段，其中所述节段可以在植物细胞中执行与完整蛋白相同或相当的功能。蛋白的功能性部分在至少50％、55％、60％、65％、70％、75％、80％、85％、90％、92％、94％、96％、97％、98％、或99％的长度上具有与所述功能性部分所源自的蛋白相同或类似(在保守性和半保守性氨基酸取代下)的氨基酸序列。
“单倍体诱导系”也意指体内单倍体诱导系。
术语“异源(heterolog)”意指所导入的多核苷酸源自例如相同物种中具有不同遗传背景的细胞或器官或另一物种，或者对原核或真核宿主细胞是同源的但是然后位于不同的遗传环境且因此不同于可能的、天然存在的相应多核苷酸。除了相应的内源基因外，可以存在异源的多核苷酸。
在本发明含义中，“同源物”应理解为具有相同系统发生起源的蛋白，“类似物”应理解为执行相同功能但具有不同系统发生起源的蛋白，而“直系同源物”是来自不同物种执行相同功能的蛋白。
“杂交(hybridizing)”或“杂交(hybridization)”应理解为这样的过程，其中单链核酸分子被添加至以最大可能程度互补的核酸链，即形成碱基配对。用于杂交的标准方法例如描述于Sambrook et al.2001。这应优选地被理解为所述核酸分子至少60％，更优选地，至少65％、70％、75％、80％、或85％，或特别优选地，90％、91％、92％、93％、94％、95％、96％、97％、98％、或99％的碱基和以最大可能程度互补的核酸链形成碱基配对。这样的添加的可能性取决于杂交条件的严格性。术语“严格性”涉及杂交条件。当碱基配对更难实现时存在高严格性；如果碱基配对较容易实现则存在低严格性。例如，杂交条件的严格性取决于盐浓度或离子强度以及温度。一般而言，可以通过提高温度和/或降低盐含量来增加严格性。“严格杂交条件”应理解为其中杂交主要仅仅在同源核酸分子中发生的那些条件。术语“杂交条件”因此不仅仅涉及所述核酸实际添加时的条件，还涉及后续洗涤步骤的条件。严格杂交条件例如是这样的条件，在所述条件下主要仅仅那些具有至少70％，优选至少75％、至少80％、至少85％、至少90％或至少95％序列相同性的核酸分子杂交。严格杂交条件例如是在4xSSC中在65℃杂交，以及随后在0.1x SSC中65℃重复洗涤共大约1小时。本文所用术语“严格杂交条件”还可以指在68℃在0.25M磷酸钠、pH 7.2、7％SDS、1mM EDTA和1％BSA中杂交16小时，以及随后用2x SSC和0.1％SDS在68℃洗涤两次。杂交优选在严格条件下发生。
“增加单倍体诱导系的诱导能力”或“单倍体诱导系的诱导能力的增加”意指具有单倍体诱导系的特性的植物的单倍体诱导率被增加。具有单倍体染色体组且获得自所述单倍体诱导系和不具有单倍体诱导系特性的相同属(优选相同物种)植物的杂交的受精种子的数目因此可以比不使用本发明的核酸获得的单倍体受精种子的数目高至少0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、或1％，优选至少1.5％、2％、2.5％、3％、3.5％、4％、4.5％、或5％，以及特别优选至少6％、7％、8％、9％、10％、15％、20％、30％、或50％，即，单倍体诱导率可以相对于之前实现的单倍体诱导率增加至少0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、或1％，优选至少1.5％、2％、2.5％、3％、3.5％、4％、4.5％、或5％，以及特别优选至少6％、7％、8％、9％、10％、15％、20％、30％、或50％。
“可操作地连接”意指在同一核酸分子中连接，使得所连接的元件以这样的方式相互定位和朝向使得核酸分子的转录可以发生。与启动子可操作地连接的DNA在该启动子的转录控制下。
植物“器官”指的是例如叶、枝条(shoot)、茎、根、营养芽、分生组织、胚、花药、胚珠或果实。植物“部分”指的是多个器官的组合，例如花或种子，或器官的部分，例如来自枝条的横切。植物“组织”例如是愈伤组织、贮藏组织、分生组织、叶组织、茎组织、根组织、植物瘤组织或繁殖组织。例如，植物“细胞”应该理解为，例如具有细胞壁的分离的细胞或其聚集物，或原生质体。
在本发明含义中，如果不是另外指明，“植物”可以是来自双子叶植物、单子叶植物和裸子植物的任何物种。这些中的许多是，例如，大麦(Hordeum vulgare)、双色高粱(Sorghum bicolor)、黑麦(Secale cereale)、黑小麦(Triticale)、甘蔗(Saccharumofficinarium)、玉米(Zea mays)、狗尾草(Setaria italic)、水稻(Oryza sativa)、小粒野生稻(Oryza minuta)、澳洲野生稻(Oryza australiensis)、高秆野生稻(Oryza alta)、小麦(Triticum aestivum)、硬粒小麦(Triticum durum)、球茎大麦(Hordeum bulbosum)、短柄草(Brachypodium distachyon)、海滨大麦(Hordeum marinum)、节节麦(Aegilopstauschii)、甜菜(Beta vulgaris)、葵花(Helianthus annuus)、Daucus glochidiatus、Daucus pusillus、Daucus muricatus、胡萝卜(Daucus carota)、巨桉(Eucalyptusgrandis)、Erythranthe guttata、Genlisea aurea、棉属物种(Gossypium sp.)、芭蕉属物种(Musa sp.)、燕麦属物种(Avena sp.)、林烟草(Nicotiana sylvestris)、普通烟草(Nicotiana tabacum)、绒毛状烟草(Nicotiana tomentosiformis)、番茄(Solanumlycopersicum)、马铃薯(Solanum tuberosum)、中果咖啡(Coffea canephora)、葡萄(Vitisvinifera)、黄瓜(Cucumis sativus)、桑树(Morus notabilis)、拟南芥(Arabidopsisthaliana)、琴叶拟南芥(Arabidopsis lyrata)、Arabidopsis arenosa、须弥芥(Crucihimalaya himalaica)、卵叶须弥芥(Crucihimalaya wallichii)、弯曲碎米荠(Cardamine flexuosa)、北美独行菜(Lepidium virginicum)、荠菜(Capsella bursa-pastoris)、小拟南芥(Olmarabidopsis pumila)、硬毛南芥(Arabis hirsuta)、欧洲油菜(Brassica napus)、甘蓝(Brassica oleracea)、芫菁(Brassica rapa)、芸苔(Brassicajuncacea)、Brassica nigra、萝卜(Raphanus sativus)、Eruca vesicaria sativa、甜橙(Citrus sinensis)、麻风树(Jatropha curcas)、大豆(Glycine max)、和毛果杨(Populustrichocarpa)。根据本发明的植物优选是玉蜀黍属(Zea)的植物，特别是物种玉米(Zeamays)，或者是高粱。
“减少表达率”或“表达率的减少”或“表达的抑制”或“减少的表达率”或相当的短语意指核苷酸序列的表达率与指定的参照相比减少超过10％、15％、20％、25％、或30％，优选增加超过40％、50％、45％、50％、55％、60％、或65％，或特别优选增加超过70％、75％、80％、85％、90％、92％、94％、96％、或98％。然而，其还可以指核苷酸的表达率被减少100％。所述表达率的减少优选地导致其中表达率被减少的植物的表型变化。改变的表型可以是介导单倍体诱导系的特性，或单倍体诱导系诱导能力的增加。
“转录率的减少”或“减少的转录率”或相当的表述意指核苷酸序列的转录率与指定的参照相比减少超过10％、15％、20％、25％、或30％，优选增加超过40％、50％、45％、50％、55％、60％、或65％，且特别优选增加超过70％、75％、80％、85％、90％、92％、94％、96％、或98％。然而，其还可以指核苷酸的转录率被减少100％。所述转录率的减少优选地导致其中转录率被减少的植物的表型变化。改变的表型可以是介导单倍体诱导系的特性，或单倍体诱导系诱导能力的增加。
与本发明相关，术语“调控序列”涉及影响表达特异性和/或强度的核苷酸序列，例如其中所述调控序列介导明确的组织特异性。这样的调控序列可以位于最小启动子的转录起始点上游，但也可以在其下游，例如在转录但不翻译的前导序列中或在内含子中。
“适于用作单倍体诱导系”意指与相同属(优选相同物种)的不具有单倍体诱导系特性的植物杂交时，植物能够产生具有单一染色体组(单倍体)的受精的种子。单倍体诱导系的特性，定义为绝对单倍体诱导率，指的是至少0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、或1％，优选至少1.5％、2％、2.5％、3％、3.5％、4％、4.5％、或5％，或特别优选6％、7％、8％、9％、10％、11％、12％、13％、14％、或15％，或非常特别优选，至少20％、25％、30％、35％、40％、45％、或50％的所述受精的种子具有单倍体染色体组。
本发明的设计和实施方案通过示例的方式，针对附图和序列进行描述。
图1：与B73(AGPv02)相比所鉴定的基因的基因组排列：
SNAREv 1(GRMZM2G179789)：在RWS花粉中增加的表达；
SNAREv 2(GRMZM2G412426)：在RWS花粉中增加的表达；
ITP(肌醇-1,4,5-三磷酸-5-磷酸酶)(GRMZM2G106834)：在RWS花粉中减少的表达；
PL(Patatin磷脂酶)(GRMZM2G471240)：编码序列中的多态性；
MITO1(线粒体输入受体)：仅仅存在于RWS中；
MITO2:与MITO1同源，但缩短。仅仅存在于RWS；
PGM(磷酸甘油酸变位酶)(GRMZM2G062320)：在RWS中缺失；
lncRNA：Pl的同源物:在RWS中缺失；
AC213048：用于序列比较的锚定基因；
MT(RNA甲基转移酶)(GRMZM2G347808)：在调控区域中的多态性。
GRMZM名字涉及AGPv02中的注释。
图2：诱导系RWS和三个非诱导系对照(NI1、NI2、NI3)中基因SNAREv 1、RNA甲基转移酶和patatin磷脂酶的RT-PCR。
图3：RWS花粉的RNASeq数据，投射到来自AGPv02的人工参照，其中SNARE和磷脂酶基因座被RWS BAC的基因座取代。(T1：转录物1，SNARE2的同源物，但具有改变的内含子结构。T2：SNARE1的同源物。编码133AA的蛋白；T3：SNARE1/2的同源物。来自图2的RT-PCR片段)。
QTL分析和候选基因的鉴定：
在玉米单倍体诱导系RWS(其被归因于诱导系Stock 6(Coe,1959))中，在染色体1(bin 1.04)鉴定到主要-QTL并精细定位。 基于这些工作，RWS中的该QTL应当进行验证以及分子分析以鉴别和功能性验证其中的基因。测试来自RWS x对照1(母体诱导系x非诱导系)的QTL定位群体的诱导能力。由此可以显示已知的QTL很可能也存在于诱导系RWS。然而，也有可能发现强的等位基因迁移取代非-RWS(对照1)等位基因。
为了分子描述所述基因座，选择了在DNA和RNA水平的多种测序方法。由于诱导系和参照基因组B73的结构差异，仅仅小比例的经典的、基于参照的测序方法获得成功。广泛的和复杂的生物信息学分析显示结构差异将然后需要通过其它技术进行检查(图1)。
在序列捕获方法中，在三个Stock6衍生的诱导系，以及RWS和5个非诱导系对照中所鉴定的QTL周围的3兆碱基被测序，且分析诱导系特异性多态性例如存在-不存在变异、SNP和InDel。最初，由此鉴定到16个候选基因，其中3个基因通过测序和分析表达数据确认：一个基因编码花药特异性patatin磷脂酶A2，其具有RWS诱导系-特异性单倍型；磷酸甘油酸变位酶，其不存在于诱导系RWS；以及RNA甲基转移酶基因，其在调控序列具有突变(图2)。
针对RWS、EMK(衍生自Stock6的另外的诱导系)和对照1开发了BAC文库，并用沿着所鉴定的QTL分布的探针筛选。针对大约150kB的靶范围(其由Dong et al.2013提及为在诱导系UH400中很可能是诱导系相关的)，提取RWS、对照1和EMK的BAC并测序。对BAC序列进行注释并与针对RWS、对照1、EMK和B73创建的全面的转录组数据进行比较。
结果，此处确认了诱导系中的缺失。因此，所检查的母体诱导系缺少在染色体1上68.26至68.36MB之间(B73参照序列的AGP Version 2)的100kB的区域。此外，在诱导系的靶区域之外出现基因类似区域的倒位和无法和B73参照基因组以及对照1比较的大的重复性序列节段。
尽管有缺失，已经鉴定的磷脂酶仍然存在于所述诱导系中，但显示强烈不同于对照的前述单倍型，以及在启动子区域的显著遗传变异。由于所述缺失，上文已经鉴定的磷酸甘油酸变位酶不再存在。
此外，在所述100kB缺失中，也鉴定到非编码RNA(lncRNA)。如所述磷酯酶一样，其是花粉特异性表达，且显示与所鉴定的磷酯酶具有82％的同源性。所述序列自身互补，即所述lncRNA形成发夹结构。非常高的表达率、与所述磷酯酶显著的同源性以及通过Sanger测序确定的低SNP密度表明该lncRNA对所述磷酯酶的调控功能。理论上，从该转录物也可以翻译出88个氨基酸长的所述磷酯酶蛋白的截短版本。
为了能够测量来自该区域的所鉴定的基因的表达水平差异，除了在DNA水平测量多态性，也实施了RT-PCR和RNASeq实验。除了作为诱导系的RWP(RWS的子系)，使用了三种遗传学上非常不同的对照品系。从这些植物收获花粉、没有花粉的花药和通过自交或杂交授粉后6-7天的胚。所述磷酯酶此处显示在来自RWP的花粉中轻微的表达增加。所述甲基转移酶在RWP的花粉中显示弱表达，而在对照的花粉中无表达。lncRNA花粉特异性表达，其也如期望的，在RWP中不存在。
另外对相同材料的花粉进行RNASeq以进一步验证前述结果。
将转录组数据(RWS的花粉RNA的RNA-Seq)投射到人工参照上，其中B73中所述QTL的区域被RWS-BAC置换。该分析显示所述磷酯酶在花粉中的表达。该基因的外显子-内含子结构对应于B73中的结构，但在5’端存在缺失，其导致终止密码子并因此导致缩短的蛋白。此外，在所述磷酯酶上游和下游检测到三种额外的RWS-特异性转录物。具有两种转录物的区域位于所述磷酯酶上游大约60kb。第一种转录物是非编码的；第二种转录物编码192个氨基酸长的蛋白，其显示与线粒体输入受体(MITO1)的同源性。在B73中，这仅仅在所述QTL(GRMZM2G174696)上游15兆碱基。所述磷酯酶下游大约90千碱基(kb)是另一转录物，其反过来显示和所述192个氨基酸长的转录物具有高度同源性。
为了获得所述QTL外的诱导系-特异性表达，在基因组范围评估RNASeq数据。出乎意料地，在上文记载的精细定位的区域之外但接近所述区域鉴别到新的候选基因，其之前很可能由于SeqCapture方法的技术限制而无法被发现。来自所述精细定位区域的所鉴定的磷酯酶上游大约400kb是基因复合物，其在RWS的花粉中，与对照相比表达显著不同(至少系数为2)。该基因复合物含有三个基因：两个基因注释为SNAREv基因，其相互具有高度同源性且在RWP中过表达，而一个基因注释为肌醇-1,4,5-三磷酸-5-磷酸酶且其表达在RWP中被减少。这些基因的克隆的转录物与公共注释有部分不同，使得它们也可以编码具有不同功能的蛋白，或也可以作为lncRNA起功能。可以从该基因座分离来自RWS的BAC并测序。该序列被整合进人工参照以在AGPv02中重新分析RNASeq数据(图3)。除了转座酶外，两种RNA(T1(SEQID No:55、56、57和63)和T3(SEQ ID No:60、61、62和65))以及具有131个氨基酸的ORF的RNA在该基因座表达(T2(SEQ ID No:58、59和64))。除了所述转座酶，全部转录物位于所述两个SNAREv基因内或之间。尽管推测起来它们本身不具有SNARE功能，它们可以参与调控同源基因。该区域的序列捕获数据显示诱导系、对照和参照基因组之间存在显著的结构差异。BAC测序确认肌醇-1,4,5-三磷酸-5-磷酸酶基因在所述诱导系的基因组水平不存在，以及来自B73的lncRNA的不存在，所述lncRNA与所述肌醇-1,4,5-三磷酸-5-磷酸酶共享转录起始位点，但从相反链阅读。从所述SNARE基因之一(GRMZM2G179789)分离cDNA也表明所述诱导系中复杂的结构改变，由于所述cDNA的一部分对应于正链而一部分对应于参照的负链。
基因功能
总之，因此可以鉴定7个基因，其对于在玉米中的体内单倍体诱导或体内单倍体诱导能力可能是重要的。
在这些对花粉管生长特别重要的四个基因中：
所述两个SNAREv基因编码已知参与泡囊运输的蛋白(文献)。在模式植物拟南芥(Arabidopsis thaliana)中，SNAREv蛋白已经被证明在花粉管顶端，其中它们参与磷酯类和果胶类的运输(文献)。在所检查的玉米诱导系中观察到的SNAREv蛋白的过表达将导致增加的花粉管生长。
模式植物烟草(Nicotiana tabacum)中能够显示磷脂酶A2也显著影响花粉管生长。因此，抑制磷脂酶A2导致花粉管生长的抑制(Kim et al.,2011)。在所检查的玉米诱导系种，所鉴定的与所述磷酯酶具有显著同源性的lncRNA的不存在可能导致所述磷酯酶基因减少的表达率或翻译率，其将促进花粉管的生长速度。
在拟南芥中肌醇-聚磷酸酯-5-磷酸酶的敲除突变体中，显示花粉管不受抑制地生长。在所检查的玉米诱导系中，肌醇-1,4,5-三磷酸-5-磷酸酶的减少的表达水平因此同样可能导致加速的花粉管生长。此处所鉴定的与肌醇-1,4,5-三磷酸-5-磷酸酶相关的lncRNA对表达率有调控作用。
因此与非诱导系相比，所检查的玉米诱导系显示所述四个基因被修饰的调控/表达率。该破坏导致显著更快的花粉管生长，其也被由于线粒体运输蛋白的表达或其调控导致的可能增加的能量代谢促进。这能够导致花粉管中的生殖细胞的运输和其生长相分离。结果是，可能出现不完全或不正确的授粉以及随后的染色体消除。
已知活性着丝粒在染色体分布中起关键作用，且通过在DNA或组蛋白水平的染色质修饰(此外，通过转录、RNA相互作用和RNA结合)而表征和修饰。所述甲基转移酶基因调控的改变可以在早期胚胎发生期间影响诱导系着丝粒的活性，其最终导致诱导系基因组在早期种子发育阶段的消除。
在所检查的诱导系中，其显示所述磷酸甘油酸变位酶基因不再存在。该基因的不存在可能负面影响花粉的能量代谢，并因此对授粉有影响。此外，所述能量代谢可以被线粒体膜蛋白影响。
所述基因中任意基因单独地或任意组合可以负责单倍体诱导的效果。
产生新的体内单倍体诱导系
为了在其它作物类型或玉米非诱导系基因型中开发新的诱导系，或为了增加诱导系基因型的诱导能力，如下进行：
在其它作物类型或玉米非诱导系基因型中鉴别相应的基因：在单子叶植物例如玉米、水稻、小麦、黑麦或大麦中，所述花粉-特异性patatin磷脂酶强烈保守，因此这些的同源物容易鉴定。与此相反，调控性lncRNA在大多数单子叶植物不存在。然而，如果它们存在，它们同样可以使用显著的同源性被发现，正如它们也存在于所检查的玉米诱导系中。在双子叶植物中，其它磷酯酶类型执行相应的花粉管生长的任务。为了鉴定这些，创建花粉或花粉管的RNA文库并针对本发明的特定磷酯酶进行筛选。在花粉中强表达的patatin磷酯酶已经能够通过向日葵花粉的RNASeq鉴定(SEQ ID NO:46-48)。
所述SNAREv基因和所述甲基转移酶基因不需要是花粉特异性的。例如，所鉴别的SNAREv基因之一(SNAREv 1)在玉米中也不是以花粉特异性方式表达的。SNAREv 1在野生型花粉中根本不表达。在注释的基因组中，可以通过BLASTP鉴定SNAREv蛋白的同源基因和功能性区域。在未注释的基因组中，将需要RNASeq数据来注释和选择SNARE基因。
同源性肌醇-1,4,5-三磷酸-5-磷酸酶或磷酸甘油酸变位酶为了用作候选基因，必须在花粉中表达。可以如上所述进行鉴定，通过BLASTP和随后的花粉中的RT-PCR或通过花粉RNASeq数据的注释。
候选基因的操作
可能的诱导系或增加的诱导能力可以通过转基因表达上述的磷酯酶和/或SNARE和/或甲基转移酶和/或磷酸甘油酸变位酶和/或lncRNA和/或线粒体输入受体实现。为此，可以从诱导系RWS克隆相应的基因，包括它们的启动子。这些基因可以被克隆进合适的转化载体并被转化进期望的植物。
花粉表达的肌醇-1,4,5-三磷酸-5-磷酸酶可以额外地或专一地通过例如RNAi减少它们的活性。例如，为此产生发夹结构，其然后包含合适的启动子和终止子，允许所述发夹结构在花粉形成的时间点或之前转录。这些发夹构建体可以被克隆进合适的转化载体并被转化进期望的植物。
可选地或额外地，可以通过TILLING、转座子诱变或其它诱变方法或“基因组编辑”产生具有稳定所述磷酯酶和/或SNARE和/或甲基转移酶、增加表达或增加活性的突变(例如在所鉴定的基因中)的植物。突变的蛋白的二级和三级结构的结构分析对此可能有用，所述突变的蛋白显示例如较致密的结构，并因此较少蛋白酶的攻击点。此外，也可以考虑所述蛋白中在泛素相互作用中起作用的区域。在所述基因活性中心的突变体可以直接测试它们的活性。为了验证所述磷酯酶的功能，已经检查了多种Tilling突变体的诱导能力。取代D74N(第74位的天冬氨酸取代为天冬酰胺)或G78R(第78位的甘氨酸取代为精氨酸)导致0.2-0.4％的母体诱导率。为了可选地或额外地操作所述肌醇-1,4,5-三磷酸-5-磷酸酶或所述磷酸甘油酸变位酶,，必须搜索敲除突变体或搜索降低所述基因活性的额外的突变体。
也可以改良Stock6衍生的诱导系。这是可能的，通过上述的转基因方法和通过导入所鉴定的候选基因中的突变。此外，有可能通过转基因或非转基因方法操作基因组中所述基因额外的拷贝，只要它们在花粉中表达。诱导能力的测试：
为了测试潜在诱导系的诱导能力，例如有如下可能：
1.向具有可见的隐性标记(例如对于玉米，有光泽的(Bordes et al.,1997)或无叶舌(Sylvester et al.,1990))的品系授粉。通过流式细胞术测试表达该特征的后代的单倍性。
2.向与所述诱导系遗传学不同，最优通过多个标记不同的品系授粉。使用这些标记以鉴定纯合植物。通过流式细胞术测试这些植物的单倍性。
采用两种可能来测试诱导能力。
参考文献
Barret,P.,Brinkmann,M.,&Beckert,M.(2008).A major locus expressed inthe male gametophyte with incomplete penetrance is responsible for in situgynogenesis in maize.Theoretical and Applied Genetics,117(4),581-594.
Bordes,J.,de Vaulx,R.D.,Lapierre,A.,&Pollacsek,M.(1997).Haplodiploidization of maize(Zea mays L)through induced gynogenesis assistedby glossy markers and its use in breeding.Agronomie,17(5),291-297.
Chen,L.,Tu,Z.,Hussain,J.,Cong,L.,Yan,Y.,Jin,L.,...&He,G.(2010).Isolation and heterologous transformation analysis of a pollen-specificpromoter from wheat(Triticum aestivum L.).Molecular Biology Reports,37(2),737-744.
Chevalier,B.S.,Kortemme,T.,Chadsey,M.S.,Baker,D.,Monnat Jr,R.J.,&Stoddard,B.L.(2002).Design,activity,and structure of a highly specificartificial endonuclease.Molecular Cell,10(4),895-905.
Coe,E.H.(1959).A line of maize with high haploid frequency.AmericanNaturalist,381-382.
Das,L.,&Martienssen,R.(1995).Site-selected transposon mutagenesis atthe hcf106locus in maize.The Plant Cell Online,7(3),287-294.
Deimling,S.,F.K.,Geiger,H.H.(1997).Methodik und Genetik der in-vivo-Haploideninduktion bei Mais.[Methods and genetics of in vivo haploidinduction in maize]Presentation Pflanzenzüchtung,38:203-224.
Depicker,A.,Stachel,S.,Dhaese,P.,Zambryski,P.,&Goodman,H.M.(1981).Nopaline synthase:transcript mapping and DNA sequence.Journal of Molecularand Applied Genetics,1(6),561-573.
Dong,X.,Xu,X.,Li,L.,Liu,C.,Tian,X.,Li,W.,&Chen,S.(2014).Marker-assisted selection and evaluation of high oil in vivo haploid inducers inmaize.Molecular Breeding,1-12.
Dong,X.,Xu,X.,Miao,J.,Li,L.,Zhang,D.,Mi,X.,...&Chen,S.(2013).Finemapping of qhir1influencing in vivo haploid induction in maize.Theoreticaland Applied Genetics,126(7),1713-1720.
Fire,A.,Xu,S.,Montgomery,M.K.,Kostas,S.A.,Driver,S.E.,&Mello,C.C.(1998).Potent and specific genetic interference by double-stranded RNA inCaenorhabditis elegans.Nature,391(6669),806-811.
Gaj,T.,Gersbach,C.A.,&Barbas III,C.F.(2013).ZFN,TALEN,and CRISPR/Cas-based methods for genome engineering.Trends in Biotechnology,31(7),397-405.
Gurr,S.J.,&Rushton,P.J.(2005).Engineering plants with increaseddisease resistance:what are we going to express？Trends in Biotechnology,23(6),275-282.
Kato,N.,He,H.,&Steger,A.P.(2010).A systems model of vesicletrafficking in Arabidopsis pollen tubes.Plant Physiology,152(2),590-601.
Kim,H.J.,Ok,S.H.,Bahn,S.C.,Jang,J.,Oh,S.A.,Park,S.K.,...&Shin,J.S.(2011).Endoplasmic reticulum–and golgi-localized phospholipase A2 playscritical roles in Arabidopsis pollen development and germination.The PlantCell Online,23(1),94-110.
Lloyd,A.,Plaisier,C.L.,Carroll,D.,&Drews,G.N.(2005).Targetedmutagenesis using zinc-finger nucleases in Arabidopsis.Proceedings of theNational Academy of Sciences of the United States of America,102(6),2232-2237.
McCarty,D.R.,Mark Settles,A.,Suzuki,M.,Tan,B.C.,Latshaw,S.,Porch,T.,...&Curtis Hannah,L.(2005).Steady t‐aste transposon mutagenesis in inbredmaize.The Plant Journal,44(1),52-61.
Odell,J.T.,Nagy,F.,&Chua,N.H.(1985).Identification of DNA sequencesrequired for activity of the cauliflower mosaic virus 35S promoter.
Prigge,V.,Xu,X.,Li,L.,Babu,R.,Chen,S.,Atlin,G.N.,&Melchinger,A.E.(2012).New insights into the genetics of in vivo induction of maternalhaploids,the backbone of doubled haploid technology in maize.Genetics,190(2),781-793.
Ravi,M.,&Chan,S.W.(2010).Haploid plants produced by centromere-mediated genome elimination.Nature,464(7288),615-618.
F.K.,Gordillo,G.A.,&Geiger,H.H.(2005).In vivo haploid inductionin maize-performance of new inducers and significance of doubled haploidlines in hybrid breeding.Maydica,50(3/4),275.
Sambrook,J.,Russell,D.W.,&Russell,D.W.(2001).Molecular Cloning:ALaboratory Manual(3-volume set)(Vol.999).Cold Spring Harbor,New York:ColdSpring Harbor Laboratory Press.
Shibuya,K.,Fukushima,S.,&Takatsuji,H.(2009).RNA-directed DNAmethylation induces transcriptional activation in plants.Proceedings of theNational Academy of Sciences,106(5),1660-1665.
Silva,G.,Poirot,L.,Galetto,R.,Smith,J.,Montoya,G.,&Duchateau,P.(2011).Meganucleases and other tools for targeted genome engineering:perspectives and challenges for gene therapy.Current Gene Therapy,11(1),11.
Sylvester,A.W.,Cande,W.Z.,&Freeling,M.(1990).Division anddifferentiation during normal and liguleless-1 maize leafdevelopment.Development,110(3),985-1000.
Till,B.J.,Reynolds,S.H.,Weil,C.,Springer,N.,Burtner,C.,Young,K.,...&Henikoff,S.(2004).Discovery of induced point mutations in maize genes byTILLING.BMC Plant Biology,4(1),12.
Twell,D.,Yamaguchi,J.,Wing,R.A.,Ushiba,J.,&McCormick,S.(1991).Promoter analysis of genes that are coordinately expressed during pollendevelopment reveals pollen-specific enhancer sequences and shared regulatoryelements.Genes&Development,5(3),496-507.
Venter,M.(2007).Synthetic promoters:genetic control through<i>cis</i>engineering.Trends in Plant Science,12(3),118-124.
Wang,Y.,Chu,Y.J.,&Xue,H.W.(2012).Inositol polyphosphate 5-phosphatase-controlled Ins(1,4,5)P3/Ca2+is crucial for maintaining pollendormancy and regulating early germination of pollen.Development,139(12),2221-2233.
Zhao,Y.,Zhao,Q.,Ao,G.,&Yu,J.(2006).Characterization and functionalanalysis of a pollen-specific gene st901 in Solanum tuberosum.Planta,224(2),405-412.
WO/2010/079430(Bonas et al.)Modular DNA-binding domains and methodsof use.
WO/2011/072246(Regents of the University of Minnesota)TAL effector-mediated DNA modification.
WO 2012/030893(Monsanto Technology LLC)Molecular markers associatedwith haploid induction in Zea mays.
序列表
<110> KWS种子欧洲股份公司
<120> 单倍体诱导系
<130> KWS0220PCT
<150> DE102015004187.8
<151> 2014-11-12
<160> 65
<170> PatentIn version 3.5
<210> 1
<211> 47944
<212> DNA
<213> Zea mays
<220>
<221> misc_feature
<222> (12578)..(12677)
<223> n is a, c, g, or t
<220>
<221> misc_feature
<222> (22322)..(22421)
<223> n is a, c, g, or t
<220>
<221> misc_feature
<222> (42238)..(42337)
<223> n is a, c, g, or t
<400> 1
atggggagca gtgaggagca tgttttttta gatcccacca gaatatgtgc atccgtgtca 60
cttcttgctc atgatctcat tggccgaatg cttaatcgag aggtctcttc aaggcccaat 120
gccaaagaag ttctccgtaa gttcaagcac ccttgtaact tgtgctttat atatatgatt 180
ctcaatttat cattgacttt tcctaatggc tttcaacaca gggcaccatg ggtcttattc 240
tacactgatt gcccgcagaa agctgaattc tctaacatat gggatactaa caaaactgca 300
gctcccatga ttcatcggga gatagtcagg tttggttact gtgagtcttc atcttcaaaa 360
tcctcaagtg acaactctga agagcgagat gaatgcggta tagttgatgc actggtgaca 420
acaataacac aggtgaggat ctcagagccc aagaggagtc ggctgttcag cctacccaac 480
gggttgttgc cgccaagcag gaacagtctc cgaacatgaa gatgatgaat ccgtgtgtgg 540
ctttctaact tgacctacct agctcccatc cccatgcatg tataaacgac atttggggaa 600
tgggtagaaa agcagagatt agggattttc gtttccgtcg gtgcagtttt ggtgttccaa 660
tggagttgcg agatgtttat gtgccttagt cttcaatttg ggggttgggg gaaaagtaat 720
tttatgtttt tgttttgtgt ctgcagattc ggaagatgga cttggaggca aggagcctac 780
agcctagcat taaggctggt ttgcttgcaa agctgaggga gtataaatct gacctcaaca 840
acgtcaagag tgagctcaag aggatatttg cgcccaatgc caggcaggct acccgggagg 900
agctcctaga gtttggaatg gctgatactc tcgctgtgag ctaatgctag gacttgactg 960
tgtctacgag actgctccta acaataaact gaagaaagca aaagaaatca ttcaacgtat 1020
tcgccgaaga gaactctaca aggtagtatg atgctttaat tgctcatata caagtgtcat 1080
tttgtcatgt cattacacat ggttaggata catacttaag tttctaacgt aggcgtccac 1140
acaacggatt ggtgcacggt tctgccgatg tatcccacgc acgtgcatgg aaggaggcag 1200
gcacccttcc ccgccgcccc ggatctcgcg ccagcccccg ccctaccccg cctgcccttc 1260
cactcttccc ccgccgcccc cggtcaacgt cacgaacccg ggcctcgtgc cgctcgtcgt 1320
ggccacactg ttcgacgagc gagtcataga gctgctgagc gtgctcgctg atgcggcggt 1380
ggggcgacca ggcaggtggt ccatcggcga agcgccatgg tcgtcgtcgg ggggcacgaa 1440
ccaggcggtg tacgcgcgcc gcgcgcccgg ctcttcatcg cctccacccg ctccagcgtc 1500
tccaccactt ccttcatcga gggccgactg cttggctcgc tggccaggca gccgagcatt 1560
agttgcgccg cttggaacgc ctgcttttgt tgatcgtttg ttttggtctg atttcagtgg 1620
gtctatccgc agagaggaag aagcagaagc tctccgagat ccaatccggc gttgaggaag 1680
ctgaatcgct ggtaaataga tgtcgcgacg cgttctgttt tggggatccc cttggctaac 1740
gggacatacg acatttgggg aatgggtaga aaagcagaga ttagggattt ttcgtttccg 1800
tcggtgcagt tttggtgttc caacagagtt gcgagatgtt tatgtgcctt agtcttcaat 1860
ttgggggttg ggggaaaagt aattttatgt ttttgttttg tgtctgcaga ttcagaaaat 1920
ggacctggag gcaaggagcc tacagcctag cattaaggct ggtttgcttg caaagccgag 1980
ggattataaa tctgacctca acaacgtcaa gagtgagctc aagaggatat ctgcgcccaa 2040
tgccaggtag gctacccggg aggagctcgt ggagtctaga atggctgata ctctcgcagt 2100
gagctaatgc taggacttga ctgtgtctac gagactgctc ctaataataa actgaagaaa 2160
gcaaaagaaa tcattcaacg tattcgccga agagaactct acaaggtagt atgatgcttt 2220
aattgctcat atacaagtgt cattttgtca tgtcattaca catggttagg atacatactt 2280
aagtttctaa cgtaggcatc cacacaatgg attggtgcac ggttctgccg atgtatccca 2340
cgcacgcgca tggaaggagg caggcaccct tccctgccgc cccggatctc gcgccagcca 2400
tcgccctacc ccgcctgccc ttccactctt ccccctgaaa gtcgcataga gggggggtga 2460
atagggcgaa tctgaaattt acaaacttaa gcacaactac aagccgggtt aacgttagaa 2520
atataaacga gtccgagaga gagggcgcaa aacaaatcat gagcaaataa agagtgagac 2580
acgatgattt gttttaccga ggttcggttc ttgcaaacct actccccgtt gaggtggtca 2640
caaagaccgg gtctctttca accctttccc tctctcaaac ggtcacttag accgagtgag 2700
cttctcttct caatcaaacg gaacacaaag ttcccgcaag gaccaccaca caattggtgt 2760
ctcttgcctt ggttacaatt gagtttgatc acaagaagaa tgagaaagaa aagaagcgat 2820
ccaagcgcaa gagctcaaat gaacacaaat gtcgctctct ctagtcacta tttgatttgg 2880
agtgattccg gacttgggag aggatttgat cttttggagt gtctagaatt gaatgctata 2940
gctcttgtaa tatgttgaag gtgggaaact tggatgccat tgaatgtggg gtggttgggg 3000
tatttatagc cccaaaacac caaaaaaggc cgttggaagg ctgctctcgc atggcgcacc 3060
ggacagtccg gtgcgccagc cacgtcagca gaccgttggg gttcgaccgt tggagctctg 3120
acttgtgggg cctctgggct gtccggtggt gcaccagaca ggtcctgtag gatgtctggt 3180
gcgccaactg cacgtgctct gtcctctgcg cgcgcaggcg cgcattaaat gcgttgtagt 3240
caaccgttgc gcgcgaagta gccattgctc tgctggcaca ccggacagtc cggtgaatta 3300
tagcggagcg ccctctgatt ttcccgaagg tagcgagttc agcttcgagt gccctggtgc 3360
accggacact gtccggtgcg ccaaaccagg gtgccttccg ggatgtcttt tgctctcttt 3420
gtttgaaccc tttcttggtc tttttattgg cttattgtga acctttgaca cctgtaaaac 3480
ttatagacta gagcaaacta gttagtccaa ttatttgtgt tggacaattc aaccaccaaa 3540
atcaattagg aaataggtgt gagcctaatt ccctttcaat ctcccccttt ttggtgattg 3600
atgccaacac aaaccaaagc aagtatagaa gtgcataatt gaactagttt gcataatgta 3660
agtgcaaagg ttacttagaa ttgaaccaat aaatattttc ataagttatg catggattgt 3720
ttctttattt tcatcatttt ggaccacgct tgcaccacat gttttgtttt tgcaaatcct 3780
tttgtaaata gtcaaaggta aatgaataag attttgagaa gcattttcaa aatttgaaat 3840
tttctccccc tgtttcaaat gcttttcctt tgacttaaac aaaactcccc cctcaaaaat 3900
cctactcata gtgttcaaga gggttttaag atatcaattt tgaaaatgct actttctccc 3960
ccttttgaat ataataagat atcaattgaa aaattcatca ttttaaaacc ttttgaaaat 4020
gggtggtggt gcggtccttt tgctttgggc taatactttc tccccctttg gcatgaatcg 4080
ccaaaaacga atacttgagt gaaatataag cccctttaac tactttctcc tgctttggcg 4140
aacataatat gagtgaagat tataccaaag ttggagagtt gcttgaagcg atggtgaagg 4200
atgagttatg gagtggaggt taagcctttg tcttcgccga agattccaat tccctttcaa 4260
tacacctatg acttggttga aaatatactt gaaaacacat tagtcatagc acatgaaaga 4320
gatatgatca aaggtatatt aatgagctat gtatgcaaga catcaaaaga aattcctaga 4380
atcaagaata tttagctcgt gtctaagttt gttcatctag tggcttggta aagatatcag 4440
ctaattgttc cttagtgtta atataggcaa tctcgatatc tccctttttt tggtgatccc 4500
ttaggaaatg ataccgaatg gctatgtgtt tagtgcggct atgctcaacg ggattatccg 4560
ccatgcggat tgcactctca ttatcacata gaagaggaac tttggttaat ttttaaccat 4620
agtccctaag ggtttgcctc atccaaagta attgtgcgca acaatggcct gcggcaatat 4680
actcggcttc ggcggtagaa agagctacgg aattttgctt ctttgaagcc caagacacca 4740
gggaccttcc caagaactgg caagtcctcg atgtactctt tctattaatt ttacaccccg 4800
cccaatcggc atccgaataa ccaatcaaaa tcaaaatgtg gatcccgtag gataccaaag 4860
cccaaactta ggagtatgaa ctaaatatct caagattcgt tttacggccg taaggtgagc 4920
ttccttaggg tcggcttgga atcttgcaca catgcatacg gaaaagcata atatccggtc 4980
gagatgcaca taaatagagt aaagagccta tcatcgaccg gtataccttt ttgatcgacg 5040
gatttacctc ccgtgtcgag gtcgagatgc ccattggttc ccatgggtgt cttgatgggt 5100
ttggcatcct tcatcccata cttgtttaga atgtcttgaa tgtacttcgt ttggctaatg 5160
aaggtgccct cttagcgttg cttcacttga aatcacaaga agtacttcaa ctcccccatc 5220
atagacatct cgaatttctg tgtcatgatc ctactaaatt cctcacatgt agattcatta 5280
gtagacccaa atataatatc atcaacataa atttggcata caaacaaatc attgtcaaga 5340
gttttagtaa ataaagtagg atcggccttt ccgactttga aaccattagt gataaggaaa 5400
tctctaaggc attcatacca tgctcttggg gcttgcttga gcccataaag cgccttagag 5460
agtttatata cgtgattagg gtactcacta tcttcaaagc cgggaggttg ctcaacatag 5520
acctcttcct tgattggtcc gttgaggaag gcacttttca cgtccatttg ataaagcttg 5580
aagccatggt aagtagcata ggcaagtaat atgcgaattg actcaagcct agctacgggt 5640
gcataggttt caccaaaatc caaaccttcg acttgtgaat aacccttggc cacaagtcgg 5700
gctttgttcc ttgtcaccac accatgctca tcttgtttgt tgcggaagac ccacttggtt 5760
cctacaacat tttggttagg acgtggaact aaatgtcata cctcattcct agtgaagttg 5820
ttgagctcct cttgcatcgc caccacccaa tccgaatctt gaagtgcttc ctctaaccta 5880
tgtggctcaa tagaggaaac aaaagagtaa tgttcacaaa aatgagcaac ccgagatcta 5940
gtagttaccc ccttatgaat gtcgccgagg atggtgtcga cggggtggtc tcgttggatt 6000
gcttggtgga ctcttgggtg tggcgggcgt tgctcgtcct ccttgtcttg atcatttgca 6060
tctccccctt gatctatgcc gtcatctaga ggtggctcat ttgattgatc ttcttcttca 6120
tcaacttgag cttcatcctc attttgagtc ggtggagatg cttgcatgga ggaggacggt 6180
tgatcttgtg tatttggagg ctcttcggat tccttaggac acacatcccc aatggacatg 6240
ttccttagcg cgatgcatgg agcctcttca tcacctatct catcaagatc aacttgctct 6300
acttgagagc cgttagtttc atcaaacaca acgtcacatg aggcttcaac tagtccagtg 6360
gacttgttaa agaccctata tgcccttgtg tttgagtcat aaccaagtaa aaagccttct 6420
acagtcttag gagcaaattt agattttcta cctcttttaa caagaataaa gcatttgcta 6480
ccaaaaactc taaaatatga aatattgggc tttttaccgg tgaggagttc gtatgatgtc 6540
ttcttgagga ttcggtgtag atataaccgg ttgatggcgt agcaagcggt gttgactgcc 6600
tcggcccaaa accgatccga agttttgtac tcatcaagca tggttcttgc catgtccaat 6660
agagttcgat tcttcctctc cactacacca ttttgttgtg gcgtgtaggg tgaagagaac 6720
tcatgcttga tgccctcctc ctcaagaaag ccttcgattt gagagttctt gaactccgtc 6780
ccgttgtcgc ttctaatttt cttgatcctt aagccgaact cattttgagc ccgtcttaag 6840
aatcccttta aggtctcttg ggtttgagat ttttcctgta aaaagaatac ccaagtgaag 6900
cgagaataat catccacaat aactagacag tacttactcc cgccgatgct tatgtaagcg 6960
atcgggccga atagatccat gtgtaggagc tccagtggcc tgtcggtcgt cattatgttc 7020
ttatgcggat gatgagagcc aacttgcttc cctgcttggc atgcgctaca aatcctgtct 7080
ttctcaaaat aaacatttgt caatcctaaa atgtgttctc cctttagaag cttgtgaaga 7140
ttcttcatcc caacatgtgc aagtcggcga tgccagagcc aacccatgtt agtcttagca 7200
attaagcaag tgtcgagttc agctctatcg aaatctacca agtatagctg accctctaac 7260
actcccttaa atgctattga atcatcactt cttctaaaga cagtgacacc aacatcagta 7320
aaaagacagt tgtagcccat ttgacacaat tgggatacag aaagcaaatt gtaatctaaa 7380
gaatctacaa gaaaaacatt ggaaatagaa tggtcaggag atatagcaat tttacccaat 7440
cctttgacca aaccttgatt tccatcccca aatgtgatcg ctctttgggg atcttggttt 7500
ttctcatatg aggagaacat ctttttctcc ccagtcatgt ggtttgtgca cccgctgtcg 7560
agtatccagc ttgagccccc ggatgcataa acctacaaaa taattttagt tcttgatttt 7620
aggtacccaa atggttttgg gtcctttggc attagacaca agaactttgg gtacccaaac 7680
acaagtcttg gagcccttgt gcttgccccc aacatatttg gcaactacct tgccggattt 7740
gttagtcaac acataagatg catcaaaagt tttgaatgaa atgtcatgat catttgatgc 7800
actaggagtt ttctttctag gcaacttggc acgggttggt tgcctagagc tagatgtctc 7860
acccttatac ataaaagcat aattaggacc agagtgagac ttcctagaat gaattctcct 7920
aattttgttc tcgggataac cggcagggta taaaatgtaa ccctcgttat cctgaggcat 7980
gggagccttg cccttaacaa agttggacaa tcttttagga ggggcactaa ttttgacatt 8040
gtttcccctt tggaagccaa tgccatcttt aatgcccggg cgtctcccat tataaagcat 8100
gccacgagca aatttaaatt tctcattttc taagttgtgc tcggcaattt tagcatctag 8160
ttttgctata tgatcatttt gttgtttaat taaggtcata tgatcatgaa tagcattaac 8220
atcaacatct ctacatctag tacaaataga tacatgctca acagtagatg tagagggttt 8280
gcaagaatta agttcaacaa tcttagcatg aagaatatca tttttatccc taagatcgga 8340
aattgtagtt ttgcaaacat caaaatcttt agccttagca attaaatttt catttttctg 8400
ttctaaggct agcaagagaa atgtttaatt cttcaatcct agcaagcaaa tcatcattat 8460
tatctttagg attgggaatt gaaacattac aaacatgtga atcaacctta gcatttaaac 8520
tagtattttc atgtctaagg ttgtcaatca tctcatggca agtgcttagc tcactagata 8580
gtttttgaca tttttctact tctagggcgt aagcattttt aaccttaaca tgtttcttgt 8640
tttccttaat aagacaatcc tcttgggaat ccaaaaggtc atctttttca tgaatagcac 8700
taattaattc atttaatttt tccttttgtt ccatgttaag attagcaaaa agggtacgca 8760
agttatcctc ctcatcacta gcattttcat cactagaggt ttcatattta gtggaggatc 8820
ttgattttac cttcttcctt ttgccgtcct ttgccatgag gcacttgtgg ccgacgttgg 8880
ggaagaggag tcccttggtg acggcgatgt tggcggcgtc ctcgtcgtcg gaggagtcgc 8940
ttgagctttc gtcggaatcc cactcccgac aaacatgggc atcgccgccc ttcttcttgt 9000
agtacttctt cttctccttt cttctcccct tcttgtcgtc gccacggtca ctgtcactag 9060
atatgggaca tttagcaata aaatgaccgg gcttaccaca tttgtagcaa accttcttgg 9120
agcgggactt gtagtctttc cccctccttt gtttgaggat ttggcggaag ctcttgatga 9180
cgagcgccat ttcctcattg tcgagcttgg aggcgtctat tggttgtcga cttggtgtag 9240
cctcctcctt cttttcttcc gttgccttga atgcgacggg ttgagcttcg gatgtggtgg 9300
gttcgtcaag ctcattgatc tttctcgagc cttcgatcat gcactcaaaa cttacaaaat 9360
gcccgataac ttcctcgggg gtcattttag tatatctagg attaccacga attaattgaa 9420
cttgagtagg gttaaggaaa ataagtgatc ttagaataac cttaaccacc tcgtggtcgt 9480
cccactttat gctcccgagg ttgcgcactt ggttcaccaa ggtcttgagc cggttgtaca 9540
tgtgttgtgg ctcctcccct ttgcgaagcc gaaaccgacc gagctccccc tcgatcgttt 9600
cccgcttggt gatctttgtg agctcgtctc cctcgtgcgc ggttttgagc acatcccaaa 9660
cctccttggc gctcttcaac ccttgaactt tgttatactc ctctctactt aaagaggcga 9720
ggagtattgt tgttgcttga gagttgaagt gctcgatttg ggccacctca tcctcatcat 9780
aatccttatc ccctacggat ggtacctgtg caccaaactc aacaacatcc catatacttt 9840
tgtggagtga ggttagatga aatcgcatta aatcactcca cctagcataa tcttcaccat 9900
caaaagttgg tggtttgcct aacgggacgg aaagtaaagg tgcatgttta gaaatgcgag 9960
ggtagtgtag gggaatctta ctaaacttct tacgctcttg gcgtttagaa gttacggagg 10020
gcgcgtcgga gccggaggtt gatgttgatg aagtgtcggt ctcgtagtag accactttcc 10080
tcatcctctt ttgtttgtcc ccactccgat gaggcttgtg ggaagaagat ttttccttct 10140
tctctttgtg gtgagaagaa gatttcttct ccttcccttt gttggaggag ctcttcttct 10200
tctccctccg tttggtgcgg gactcttcca atgaagtgct ctcgttgctt gtagtgggct 10260
tttcgccggt ctccatctcc ttcttggcgt gatctcccga catcacttcg agcggttagg 10320
ctctaacgaa gcaccgggct ctgataccaa ttgatagtcg cctagagggg gggtgaatag 10380
ggcgaaactg aaatttacaa atataaacac aactacaagc cgggttagcg ttagtaatga 10440
agaaacgagt ccgcgagaga gggcgcaaaa caaatcgcaa gcaaatgaag agtgtgacac 10500
gtggatttgt tttaccgagg ttcggttctc gcaaacctac tccccgttga ggaggccaca 10560
aaggcccggg tctctttcaa cccttccctc tctcaaacgg tccctcggac cgagtgagct 10620
tctcttctct aatcaaagtt gggaacaaaa cttcccaaca agggccacca cacaattggt 10680
gcctcttgcc ttgattacaa tgggtttttg atcacaagaa caagtgcgaa agaaaagaag 10740
caatccaagc gcaagagctc aaaaagaaca cggcaaatct ctctctctaa tcactaaagc 10800
cttttgtgga attggagagg atttgatctc ttttggtgtg tctagaattg aatgctagag 10860
ctcttgtagt agttgagaag tggaaaactt ggatgcaatg aatggtgggg tggttggggt 10920
atttatagcc ccaaccacca aacttgaccg ttggctgggt tgtctgttcg atggcgcacc 10980
ggacagtccg gtgcacaccg gacagtccgg tgcccctgcc acgtcatcac tgccgttgga 11040
ttctagccgt tgaagcttct gacttgtggg cccgcctggg tgtccggtgc acaccggaca 11100
tctactgttc cttgtccggt gtgccggagt gggcgcgcct gacatctgcg cgcgcagagc 11160
gcgcattaaa tgcgcggcag agagccgttg gcgcggaaat agccgttgct ctcgagtcgc 11220
accggacagt ccggtgcaca ccggacagtc cggtgaatta tagtggacgg gccgatggct 11280
tttcccgaag ctggcgagtt cctgaggccg acctcccttg gcgcaccgga cactgtccgg 11340
tgtacaccgg acagtccggt gaattatagc ggagtcgcct ctggcaattc tcgaaggggg 11400
cgagttggag cttgagtcct ctggtgcacc ggacgctgtc cggtgtacac cggacagtcc 11460
ggtgctctca gaccagaggg ccttcggttc ccactatgct cctttgttga atccaaaaac 11520
ttggtccttt tattggctga gtgtgaacct tttacacctg tgtaatctat aaacttgtgc 11580
aaacttagtt agtccaattg tttgtgttgg gcaattcaac caccaaaatt aattagggac 11640
taggtgtaag cctaattccc tttcagtttt cccgggcggt catccataga acaggtcctt 11700
acggagaggc actcgagaaa ccgctcgagc ccccttgaag accacaagca caacatcata 11760
ataagagaag ggaaaacagc gtatcataga taatctcatc atgttcattg attagagtta 11820
agcaatagca taaagctaaa cagtaataat ccaacccaaa taggtgaaca aggacatgga 11880
taacaaaagc tagtcaatcc ttaggcataa atgtgtaaag cgggaggtga attaaataat 11940
gaataggaca tagataggtc aagggacact tgcctccacc aaccgactgc tgctcagggg 12000
cttctcctgc gggttcctcg ggctcttcaa ccggatcgtt ctctatgcga gcgcaaacat 12060
acacacatcc acatatttaa taccaaagaa cagtacacca tacaatagaa tgcaataagt 12120
aaacagacgt tccacgcggg ctcgcgagta cggttaagag agaaagagga aaagacagtc 12180
gagaaacgat cacgttgcat gattataaat tagccactag cttaatggaa ggaaatttaa 12240
tgtagacact atgtttagcg taaagtaaag tcatgtttca tgtctaatta ttataagcag 12300
gtggagacaa ataaaaggat agccgcgcgg cgagacgcgc gacaaagctc tctaaaacaa 12360
attaagaagt taacgactcg tcgcgcgact gagcacgcag cgagacactt cgccttagtt 12420
aagaggagac gttaagcgtc gcgcgacgaa gcgcacgacg gcatacgtcg actaaactga 12480
gtccaaagtg gaacgtcgcg tcaattccca cgcggcgtta caccttaaac aacctgaaac 12540
aaaatgaacg aatcaagcct gatcatccgc cccccccnnn nnnnnnnnnn nnnnnnnnnn 12600
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12660
nnnnnnnnnn nnnnnnnacc gttcaacaaa caccgcccgg ggttccaacc gaccaggctc 12720
cagatcgccc gaccgatccc ccgtcccggc ctcgcccccc cccttcttcg ctcgcccgcg 12780
ctcgcggtga ttgctcgtta agaagcgtgt tgcgtcgcgc gcttcgccgc gcgacggatt 12840
tatctaaaat tcagattcta tcctgtgttg cgtcgtgcgt ttcgtcgcgc gacgatccat 12900
tttgtttcag gttgtttaag gtgtaacgcc gcgtgtgtat tcacgcgacg ttccactttg 12960
gactcagttt agtcgacgta tgccgtcgtg cgcttcgtcg cgcgacgctt aacgtctcct 13020
cttaactaag gcgaagtgtc tcgctgcgtg ctcagtcgcg cgacgagtcg ttaacttctt 13080
aatttgtttt agagagcttt gtcgcgcgtc tcgccgcgcg gctatccttt tatttgtctc 13140
cacctgctta taataattag atatgaaaca tgactttact ttacgctaaa catagtgtct 13200
acattaaatt tccttccatt aagctagtgg ctaatttata atcatgcaac gtgatcgttt 13260
ctcgactgtc ttttcctctt tctctcttaa ccgtactcgc gagcccgcgt ggaacgtctg 13320
tttacttatt gcattctatt gtatggtgta ctgttctttg gtattaaata tgtggatgtg 13380
tgtatgtttg cgctcgcata gagaacgatc cggttgaaga gcccgaggaa cccgcaggag 13440
aagcccctga gcagcagtcg gttggtggag gcaagtgtcc cttgacctat ctatgtccta 13500
ttcattattt aattcacctc ccgctttaca catttatgcc taaggattga ctagcttttg 13560
ttatccatgt ccttgttcac ctatttgggt tggattatta ctgtttagct ttatgctatt 13620
gcttaactct aatcaatgaa catgatgaga ttatctatga tacgctgttt tcccttctct 13680
tattatgatg ttgtgcttgt ggtcttcaag ggggctcgag cggtttctcg agtgcctctc 13740
cgtaaggacc tgttctatgg atgaccgccc gggaaaacag tgcaaccatg agggtggaat 13800
ggggtgccct tagctgaata attagaggat ccggggtgta gttcacttag ccgtcgtgcc 13860
gtcaatgggg ctcggtgtat gcggctcgct ctgccaagtt tgggttcgcc ccttggggag 13920
gagtgcggtg catttaggaa acctaacggg tggctacagt cccggggaat ctttgtaaag 13980
gctatgtagt gatgccctgc tgggtcacct tggtagtgat caatggagag tcatgatctc 14040
cgggtagaat gggaatcacg gcttgtgggt aaagtgcaca acctctgcag agtgtttgaa 14100
aactgatata tcagccgtgc tcacggttat gagcggccaa gggagctcca gtgattagtg 14160
gtacttgatc agagatactt tggtacaggt ggttatgaga tcgatgattc tggttatgac 14220
tatgatgctg gtaagtggta ctctttccgt ttggaaagga gtacgtttgg gttaataact 14280
tgggttaatg ctaaaacttg gctttctatt agtaaataat aatctgacca actaaaagca 14340
actgcttgac ttatccccac ataaagctag tccactacag ccaaacagga tacttgctga 14400
gtatgttgat gtgtactcac ccttgctcta cacaccaaac ccccccccca tccccaggtt 14460
gtcagcattg caaccactgc tcagtcgaag atgaagctgt ggaaggagac ttccaggagt 14520
tccaagatta cgatgagttc taggtgtggg ttagcggcaa cccccagtcg gctgcctgtg 14580
aaggccgcgg ttatctacgt ttcttttccg cactttgatt tattgtaaga actatatgga 14640
cgtctcagac gtatgatgta atcgactatt tcccttagta atactatttt gagcactgtg 14700
tgatgatgtc catgttatgt aactgctgtg tacgtgaata actgatcctg gcacgtacat 14760
ggttcgcatt cggtttgcct tctaaaaccg ggtgtgacat aagtggtatc aaagccgtgc 14820
tgactgtagg accgctaacc tagaatagaa tggtcgctct aaggactata gacctctgtc 14880
tctgccttga ctttgatatc ccttcaaaag ttggtcatac cgaccaaacc tatgttctac 14940
tatatattat accttgctga aaatcatgtt ttattccagt ccttcattta cttatgattc 15000
attatttgct ggtcatatta attctgttct cacctttttg cttgcgatgt cttttgtaga 15060
tggctcgact tagacacact gcacgaaagt cagtcatccc cttcttaccc tcccgccttg 15120
ctgagcgtcc gcttcgccgt cccgtggccg gacagtccag ccacttggag agactacacc 15180
accgcctgcg tgaggagcag gagcgtcgac gacaggagca gcagagctct tccttctcgc 15240
tccaccagga gatagagtct gtgaggagct gctcccctgt gcttcctctg gagccgcccc 15300
ctccaccacc actgggcgcc ccagcttctg gagtagctgc tggaggagac ccagacgatg 15360
gagatggcga cgacagctcg agccacgaca ccgacttctc tgctaacctt gagccggaag 15420
gatgggttac tcgacccatc actcgcgacg ctgctcgcgg gtgtcacttc cacgatgcgc 15480
tcgacaccct gctacgtcgg gcatttaacc agcatacttg gtctgtcgag tatcgctgtg 15540
tggtctacca gcacagtcgc ggggtctacc cggaccgctg ggaggcaacc tgcttggtgc 15600
gctgcccgga gaacagtctc cagggtgcgg aggcctgctc agagcactat tctatctctg 15660
agcgggactc agctgaggca gccatgcaag atgctgcacg gcgtgcgctt tcgcactact 15720
gctcggtttt cggtggggca gctgacggtc ttgacctgaa gtattacccc cgccgtccat 15780
ctggcagcac aggaggcgtg attgtctcac ctgtcggtga gggcaatcct aggttgagca 15840
gcacagtcaa cctagccgcc gtgctaaaca cggagctgga ccatgcatta gacgagctga 15900
gtagggctcg tgctgagatc gccctgctgc gggctgagcg cgcggaacgt cgtcacctgg 15960
atggtggttc ccccgctccc gtcgggactc agcacccgta ccgctcacct cagcgtggac 16020
accagtctta tggcaatccc gcctgcaaga ccaagataac tctagaacca tatatcgtta 16080
gagttggatc ttgtaattaa tacgaaatat atacatagaa gcttcagtct tagcgttagt 16140
ctcggtctta gttagtctta gttaaacagg gtagtttgct atatcctgtg catttatgtt 16200
tgtcatgatg aactatgttt ggtttggatc tttgtaatga ttgtcaccag agtgtgggta 16260
ccccctgcat tttggtttac ctattatgtt aatagagtta gttatatagt tgggaaacct 16320
tttattccac tctcctcttt atctgagaaa ctgtgtggtc tgtgttggag atcagtgaag 16380
atgctcatct gttcagtgct gttgaagaat tctattctct tttcttatgc tgcaagattt 16440
gccagatcag tcctgatgtg tggttgcatt ctgcagatgt cagagaacag gcgcagagga 16500
ggaaggcgtg ctcagcagga gcgagccgct caacaggagg aggtgcccca gcagcagcac 16560
ctgccgcccc cgcccccgat gtccatcgag cagatgtttc tgatgcagac tcaggcagtt 16620
caagccatcg gtcagactct ggccgccatt cagcagcagc agcagcagca ggccccacct 16680
caacctcaga tgcctcagat gcccagagac aagcgtgctg aattcatgag aggtcatcca 16740
ccaacgttcg ctcattcttc tgaccctatg gatgctgaag attggctgcg cactgtggag 16800
cgggagttgc ataccgctca gtgcgatgac agggagaaag tcctgtatgg tccccgtctg 16860
ttgagaggag cagcccagtc atggtgggag tcttacctcg ccacccatgc ccatcctgac 16920
gccatcacct gggaagagtt cagaggtagc tttcgtcagt accatgttcc tgcaggtctg 16980
atgacagtga agaaggagga gttcctggcc ctcaaggaag ggccattgtc tgtcagtgag 17040
taccgagaca ggtttctgca attgtctcgc tatgctcctg aagatgtcaa caccgacgcc 17100
aagcgacagt accgtttcct gagaggcttg gttgaccctc tgcagtacca actgatgaat 17160
cacaccttcc cgacattcca gcacctgatt gacagagcaa tcatgacaga aggaagcgta 17220
aggagatgga agatcgtaag cgcaagatca gtggacccca gcctggaagc agcaatcgtc 17280
ctcgtttctc aggcaatcaa cctcagcagt tcaggcagaa ccagcgtcca cctcagcagc 17340
agcagcaatt ccaaaggcag tatcctcagc accagtacca gaaccgtcag agcaatcagt 17400
caggaggtca gtttcagagg cagaatcagc aagcacctcg tcttcctgcc ccagcaaatc 17460
agcagaacag tcaggcagca ccagctcagg ttggaaacag agcatgtttc cactgtggag 17520
agcaaggcca ctgggtgatg caatgtccga agaaggcagc ccagcagcag tcaggcccca 17580
atgccccagc gaagcagaat gtgcctcagc ctggagcagg caatcgctct cagccgcgct 17640
ataatcatgg aaggctgaac cacttggagg ctgaagcagt gcaggagacc cccggcatga 17700
tagtaggtat gttcccagtc gactcccata ttgcagaagt gttatttgat actggagcaa 17760
cgcattcttt cattactgca tcatgggtag aagcacataa ccttccaatt actaccatgt 17820
caacccccat tcaaattgac tcagccggtg gtagaattcg agccgatagc atttgtttga 17880
atataagtgt ggaaataagg gggatagcgt ttcccgccaa ccttatagta atgggtactc 17940
aggcaataga tgtcatccta gggatgaatt ggctagataa gtatcaggca gttatcagtt 18000
gtgataaaag gaccatcaag ttggtgtccc cactaggaga ggaagtggtg accgagttag 18060
tcccgcctga gccaaagaaa ggaagttgtt atcagatagc tgttgatagc agtgaagcag 18120
acccaatcga gaggatcaag gttgtgtccg agttcccaga tgtgtttcca aaggacttac 18180
cgggtatgcc accagagcgg aaagttgagt ttgctataga gcttcttccc ggaaccgccc 18240
ctatctttaa gagagcttac agaatatctg gaccagagtt ggttgagctt aagaagcaga 18300
ttgatgagct gtcagagaaa ggttacattc ggccaagcac ctcgccttgg gccgcccctg 18360
tcctatttgt ggagaagaaa gatggcacca agaggatgtg tatcgattat cgagctttga 18420
atgaagtcac gatcaagaac aagtatccct tgcccagaat agaagatttg ttcgaccagt 18480
tgagaggagc cagtgtgttc tccaagattg atctgaggtc aggttatcat cagctcagga 18540
tccgaccttc ggacattccg aagacggcat tcatttccaa gtatggtttg tatgagttca 18600
cagtgatgtc ttttggtttg accaatgcgc cagcgttctt catgaacttg atgaacagtg 18660
tattcatgga ttatctcgat aagtttgtgg tggtattcat tgatgacatt ctggtttatt 18720
ctcaaagcga agaagagcac gcagatcatt tgaggttggt attgcagaga ttgcgagagc 18780
atcagttgta tgcaaagttg agcaagtgtg agttctggat cagtgaggtc ctgttcttgg 18840
gtcacataat caacaaagaa ggattggttg tggatccgaa gaaagtggca gacattttga 18900
actggaaagc gccaacagat gctagaggaa tcaagagttt cattggaatg gccggatact 18960
atcggcgatt cattgaaggg ttttcgaaga tcgcaaaacc aatgacagcg ttgctaggca 19020
acaaagttga gttcaagtgg acccagaaat gtcaagaggc ctttgaagcg ctgaaagaga 19080
agttgactac agcgcctgtc ctagtcttgc ctgatgtgca caagcccttc tcagtgtatt 19140
gtgatgcttg ttacacaggt ttgggatgtg tgttgatgca agagggaaga gttgtggctt 19200
actcgtcccg acaactgaag gttcatgaga agaattaccc aatccatgat ctagagttgg 19260
cagcagtggt tcacgcactg aagtcatgga ggcactatct gtatggacag aaatgcgatg 19320
tttacacaga tcacaagagt ctgaagtaca tattcactca gtcagagttg aacatgaggc 19380
aacgaagatg gttagagttg atcaaagatt atgagttgga gattcattac catccaggca 19440
aagcaaacgt agtggcagat gctttgagca gaaagagtca agtcaatctg atggtcgctc 19500
gtccgatgcc ttatgagttg gccaaagagt ttgacaagtt gagtctcggt tttctgaata 19560
attcgcgagg agtcaaagtt gagttggaac ctaccttgga gcgcgaaatc aaagaagcgc 19620
agaagaatga tgagaaaatc agcgagatcc ggcgactgat tctagatggc aaaggcaaag 19680
aatttcgaga agatgcagaa ggcgtgatat ggttcaaaga ccgcttgtgt gttcctaatg 19740
tccagtctat tcgggagttg attctcaagg aagctcatga gacgtcctat tcgattcacc 19800
ctggcagtga gaagatgtat caggatctga aaaagaaatt ctggtggtac ggaatgaaga 19860
gggagatcgc agagcatgtg gctaggtgcg atagttgccg aagaattaag gcagagcacc 19920
agagacctgc tggattgttg caaccattgc agatccctca gtggaaatgg gacgaaatcg 19980
gtatggattt catagtcgga ttgcctcgca ctcgagccgg ctacgattcc atctgggtag 20040
tagtggaccg cttgaccaag tcagcccact tcatacctgt caagaccaac tacagcagtg 20100
cagtattggc agaattgtat atgtctcgga tcatttgtct tcatggtgtg ccaaagaaga 20160
tagtgtcaga cagaggaacg cagttcacct ctcatttctg gcagcagttg catgaagctt 20220
tgggcacgca tctgaatttc agttcagctt atcatccaca gacagatggc cagaccgaaa 20280
ggaccaacca aattcttgaa gatatgttga gagcctgtgc gttgcaagat cagtccggat 20340
gggataagag attgccttat gcagagtttt cctgtaacaa cagttaccag gccagcttga 20400
agatgtcacc atttcaggcg ctctatggaa ggagttgtag aactccgttg caatgggatc 20460
agcctggaga aaagcaagtg tttgggccag acattttgct tgaagccgaa gagaacatca 20520
agatggtccg agagaatctg aagatagcgc aatcgaggca gcgaagctat gcagacacaa 20580
gaagaagaga gctgagtttc gaagtcggag actttgtcta tctgaaagtg tcaccaatca 20640
gaggagtcag aaggttcgga gtgaaaggca agctagcacc ccgctacatt ggtccgtatc 20700
agattcttgc aagacgtgga gaagtggcct atcagctcag tttgccagag aatttgtctg 20760
ctgtgcatga tgtctttcat gtgtctcagt tgaagaagtg cttgcgtgtg ccagaagagc 20820
agttgccagt agaaggtctt gaagtccagg aggacttgac ctacgttgag aagccagtgc 20880
aaatccttga ggttgcagac cgagtcacct ggaggaagac catcagaatg tgcaaagtca 20940
gatgggatca tcactctgag gaagaagcaa cctgggagcg tgaagatgat ctgatggcca 21000
agtaccctga gctctttgct agccaaccct gaatctcgag ggcgagattc ttttaagggg 21060
gataggtttg taacgccctg aatttggggg tagaattttt cttcttttct ctcaccaaat 21120
tcgggcgtta ctctcttttc tctttccccg tttgctcctt cttcccaatt tcaaaccagt 21180
atagcggcag gtgtccgtgt catgtataaa ccaaaaccta agtgtcatgg gtgttgcatc 21240
atgccgaagc acatttcttt gtctgatgtt gagtgttcgt ctcgttccgt tccggatttc 21300
ggttcgcgat ttaattccgt ttagtggtcc gcgctcgtcg cgggttttcg atccgcgaag 21360
tggcccgacc catcccaacc tagtccagcc cagcccagcc ggcccggccc gccccggcct 21420
gcgcgcccct ggcgcccaaa cccccccatg cgccccccct cctctctctc tctcatttgg 21480
atctcccgcg caacaacctc tcctctccct cttccacctc tctctccccg tggtgcccta 21540
ggatttggag acggcgatca ccggattttg gaccccgagg tgagctcccc tcccctcccc 21600
ttctcttctc tctctctctc cctcctcttc ttctccccac gcgcgccccc cttctccccc 21660
tgctcacgcg cgcccctgcc cgcccccgct caccggcggc gcggcgcccc ccgcccctgc 21720
ccggccccgc gcggcggcgc ccgccctccc ccggccgcgg cggcgctcgt ccgccctcac 21780
ccccggccgc ggcgcccgcc cctccccctc gcgcgcggag gcggcgcccg ccctcaccac 21840
gacccgcagc gaccccgccc cgtccccgcc tctccccccg cgcgcggcgg cgcccgcccc 21900
ccgcccctcc ccgcggcggc gctcgcccgc ccgcccctgc ccctccccgc ggcggcgccc 21960
gcccgcccct gctcgcgcgc agcgcggccc cggcgcgccc ccggcatggc ccggcgcggc 22020
cccggtggcc cctgctcgcc ggcgcgaccc cggcgtggcc cccagccccc ggcgcgtccc 22080
cggcgcggcc ccggccggct cggccgcccc tggccggttc aaccgccccc cctggccggt 22140
tcaaccgccc tggccccagc tcgcccgccc gttcccccgt cccggcctcg cccgaccgta 22200
tcttcgctcg cccgcgctcg cggtgattgc tcgttaatta gcgtgttgcg tcgcacgcta 22260
cgccgcgcga cggatttatc taaaattcag attctatcct gtgatacgta gtacaactga 22320
cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 22380
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgggtcggc gctgagatca 22440
gcttgattcg tttttggtta tacatgacac ggacacctgc cgctatactg gtttgaaatt 22500
gggaagaagg agcaaacggg gaaagagaaa agagagtaac gcccgaattt ggtgagagaa 22560
aagaagaaaa aattctaccc ccaaattcag ggcgttacat caggctacaa aggatgccaa 22620
tggtattgct gctctctata ttgttcttgt tctaatgtaa aaactacaac acaactcttt 22680
acttgatccc agaaattcct tctgcctcaa atggagacaa tgacgagtgg tcataagtac 22740
agagattgca gacaaggtaa attttgcaat agaaataact aaccaaccat tagtgcttga 22800
aaaaaactgg actggtgact ggggcacgtg gtttcatcaa catttggacc tcaacggtct 22860
aatcagtata acttagaagt tggctagctc ttgaaaaaca ctgcatgaca ctaagcattt 22920
gtttattttc agctgcttac acccctatga tttcaagtaa ctacttgtct acttgtgata 22980
atcacctgaa tatgattatt tgaaatgctt atcatgtctc gtcaattgca tttcttttat 23040
gtgtacctga agtctgctct tgcttcctaa tagagttcgt tttttaatac agaaaccact 23100
ctgagatagc cacaatatag taaaagtggc agctaaggta ctaaaaacac ccatgcaaat 23160
aagaaaaaaa tgaatcttgt attttaattt tgttaaatac ctctatagtt tggcgatata 23220
ttatgttacc atcctgctta tagcctgtag gtcattttat atgagccatc aaattgcgat 23280
gacagttgcc acaaatccag tttcatatga aggtattagc tgtgtaacaa gctaattgtt 23340
gctctctgcc caataagtta ttcaattgga ttagtaggtt gcatccaagg ttattcaatt 23400
ggatcagtag gttgcatcca aggtatactg ctgctctctg cccaataagt tattcaattc 23460
gatcagtagg ttgcatgttc ccttcatttt attaaaaaat acataataat ataataagta 23520
cttgtttgtt ctaaaaataa tacttctgta aatgaggata ttaattttcc ttttggtaat 23580
aatgcaggtt gatgatactg aagtcatcag ttttttgctg caaactgaaa taattcctct 23640
gtgcttgcga accatggaga tgggtagtga gctatccaaa attgtatgta gctagccaaa 23700
tattctcatt caaatatcat aatttatctc ttctgcttaa tactggcaaa ggtgtaatag 23760
tttttttagt attgatttgt cacctgaagt ttatcttgtg cactactact ttgccatcat 23820
cagttatctc tagaatactc ttgtcctgta ccattttctc tctgataagc ctaaatttgt 23880
acaattcata agcctaaaag gtgacttata taatatatac aaggaccctc aagagttgtt 23940
tggcaattca gtgactgtcc tgggtcctgt tttggggagc ttctggtagc ttttgcttct 24000
ccaaaagaaa agctagaagc tccccccaaa cagagcagct tcttcaagcc ggtaaaagct 24060
tcaaaagctg taattatact aaaaacagtg aagctccctc agagcagctt cccagctctc 24120
taggagatgc ttttggagaa gctacagttt ccccaaacag ggccctgctc tgttgaaccc 24180
cccccttcct gatacatatt tgaatatgag tttatagtgt gtgtgggggt gtaagtaggg 24240
gggtaatggg ttctaaattt tatactataa aaattaagga tcggattaga attgagctct 24300
atttctattc atttttgaac taaaattaat taagggctca aatgaattat gaagaagcat 24360
taggatcatg atccattacc acccctacgt gtaagatgtt ttttggtggt tgtggttgat 24420
tttgaatttt aaggccgcat atgtctcatg gaccacacaa gctcatattc atctacattt 24480
gtagccgtca ctaacttagc caaatatgca tatgtggcgg ctagcaacag gtccttggtt 24540
tcttgggtta tttattctct ttttatcgtg tttgaatgtt ttcgtgttca tttgcataac 24600
atcttaggtc tacattagta tatgaattga gatcaaatgt gaattggacc acacaagctc 24660
atattcatct acatttgtag tcgtcactaa cttagccaaa tatgcatatg tccgcttctg 24720
atttcattgt gtcttttctt caggagtttg gggatcaagg agaggactcc attatcttgt 24780
caccgcgact gaaggagatt agtactcctg accgccccac tgccctccgt ttcctaggta 24840
cacgcataac agccattggt atgaatacat gttttatacg tgaatggagt tccagtttaa 24900
tttaaagatt caagttcact acaacaagat tttacagtac tgagcccatt tgactttcct 24960
tgagaaatag tgaaagggaa ttaggcttac acctagttcc taaataattt tggtggttga 25020
attgcccaac acaaataatt ggactaacta gtttgctcta gtgtacaagt tatacaggtg 25080
ccaaggttca caacaagcca attaaaaaga ccaaagttgg gttcaaaata gagagccaaa 25140
ggcatcccga aaggctccct ggtttggcgc accggactgt ccggtggcgc accggacagt 25200
gtccggtgca ccaggggacc tcgcgcagaa ctcctcagcc tcgggaattt ttcggagccg 25260
ccgcgctata attcaccgga ctgtccggtg tacaccggac agtgtccggt gctccaagaa 25320
aacgcggctc cggaacttgg cagcctcagg aaatcagaac ggctgctccg ctataattca 25380
ccagacatgt ccggtgtaca ccggactgtc cggtgcaact gcggagcaac ggctacttcg 25440
cgccaacggt cacctgcagg cgcattcaat gcgcgccaga agcgcgcaga agtcaggcac 25500
acccatgctg gcgcaccgga cactctacag tacatgtccg gtgcgccacc ggacatcaag 25560
gcgggcccag aagacagaac tccaacggtc aaattccaac gactttggtg acgtggctgg 25620
cgcaccggac tgtccggtgc accatatgac agacagcctc caccaacggt catgtttggt 25680
ggttggggct ataaataccc caaccacccc accattcatt gcatccaagt tttccagctt 25740
ccaaccacta tataagagct agcattcatt gcaaagcaca ccaaaagaga tcaagtcctc 25800
tcccaactcc acacaaagcc ttagtgatta gagagagtga tttgtagtgt tcatttgagc 25860
tcttgcgctt ggatcgcttc ttttctttgg cattctttct tgtgatcaaa cactcacttg 25920
taattgaggc aagagacacc aattgtgtgg tggtccttgc gggaagtttg attcccaagt 25980
gatttgagaa gagaagctca ctcggtccaa gggaccgttt gagagaggga agggttgaaa 26040
gagacccggc ctttgtggcc tcctcaacgg ggagtaggtt tgagagaacc gaacctcggt 26100
aaaacaaatc cacgcgtctc acttcattat tcgcttgcga tttgttttca cgccctctct 26160
cggactcgtt cttatttcta acgctaaccc ggcttgtagt tgtgtttata tttgtaaatt 26220
tcagtttcgc cctattcacc ccccctctag gcgactatca attggtatca gagcccggtg 26280
cttcattaga gcctaaccgc tcgaagtgat gtcgggagat cacgccaaga aggagatgga 26340
gaccggcgaa aagcccacta caagccacgg gagcacttca tcggaagagt cccgcaccaa 26400
gaggaaggag aagaaagact cctccaaagg gaaggagaag aagaaggact cctccaaagg 26460
aaaggagaag aaatcttctt cacacaaaga aaagaaggag aagtcttcct cccacgagcc 26520
gcaacggagt ggggacaaga aaaagaggat gaggaaagtg gtctactacg agaccgattc 26580
ttcatcgaca tccacctctg gctccgacgc ggcgtccgtc acttctaaac gccaagagcg 26640
taagaagtat agtaagattc ccctacgcta ccctcgcatt tctaaacata cacctttact 26700
ttccgtccca ttaggcaaac caccaacttt tgatggtgaa gattatgcta ggtggagtga 26760
tttaatgcga tttcatctaa cctcactcca caaaagtata tgggatgttg ttgagtttgg 26820
tgcacatgta ccatccgtag gggatgaaga ctatgatgag gatgaggtga cccaaatcga 26880
gcacttcaac tcccaagcca caaccatact cctcgcctct ctaagtagag aggaatacaa 26940
caaggtgcaa gggttgaaga atgcgaaaga aatttgggat ctactcaaga ccgcgcacga 27000
gggtgatgaa ctcaccaaga ttaccaagca ggaaacgatc gagggggagc tcagtcgctt 27060
ccgtcttcgc caaggggagg agccacaaga tatgtacaac cggctcaaaa ccttggtgaa 27120
ccaagtgcgc aacctcggga gcaagaaatg ggatgaccac gaggtggtta aggttattct 27180
tagatcactc atcttcctta accccactca agttcaatta attcgtggta atcctagata 27240
tacactaatg acccccgagg aagttattgg gaattttgtg agctttgaat gtatgatcaa 27300
gggctcaaag aagatcaacg agcttgatga tccctccacg tccgaagcac aaccggtggc 27360
tttcaaggcg acggaggaga agaaggagga gtctacacca agtagacaac caattgacgc 27420
ttcaaagctc gacaacgagg agatggcttt aatcatcaaa agctttcgcc aaatcctcaa 27480
gcaacggaag gggaaggatt acaaatcccg ttcaaagaaa gtttgctaca agtgtggtaa 27540
gcccggtcac tttattgcta aatgtccatt atcaagtgac agtgacaggg ataatgacaa 27600
gaagggcaag aggagagaaa agaagaggta ccacaagaag aggggcggtg atgcccacgt 27660
atgccgcgag tgggactcca acgagagctc caccgactcc tccgacgacg aggacgtcgc 27720
caacatcgcc gacaccaagg gactcctctt ccccaacgtc ggccacaagt gcctcatggc 27780
aaaggacggc aaaaacaaga aggataaatc taaatcctcc actagatatg aatcctctag 27840
tgatgaaaat gttagtgatg aggaagataa cttgcgatct ctttttgcca acctcaacat 27900
gcaacaaaaa gagaaactta atgaattgat tagtgtcatt catgaaaagg atgatctctt 27960
ggacacccaa gaggacttcc ttattaaaga aaataagaag catgttaagg ttaaaaatgc 28020
ttatgctcta aaagtagaaa aatgtgaaaa attgtctagt gagctaagca cttgccatga 28080
gactataaac aaccttagaa atgagaatgc taatttgtta gctaaggttg attctcatat 28140
ttgtaatgtt tcaagttcca atcctagaga taataatgat gatttatttg ctaggattaa 28200
agatttgaac atttcacttg ctagccttag aaatgaaaat gaaaaattgc ttgctaaggc 28260
taaagatttt gatgtttgca atgttactat ttctaacctt agaagtgaaa acgacatatt 28320
acatgctaag gttgtagaat taaaatcttg caaacctcct acatctatag ttgagcatgt 28380
atctatttgt actagatgta gagatattga tgttgatgct attcatgatc acatgacttt 28440
aattaaacaa caaaatgatc atatagcaaa actagatgct aaaattgccg agcataactt 28500
agaaaatgaa aaatttaaat ttgctagaag tatgctctat agtgggagac gccctggcat 28560
caaggatggc attggcttcc aaaggggaga caatgtcaaa cttaatgccc ctcctaaaag 28620
attatctaat tttgtaaagg gcaaggctcc catgcctcag gataacgagg gttacatttt 28680
gtaccctgcc ggttatcccg agagcaaaat taggaggatt cactctagga agtctcactc 28740
tggccctaac catgctttca tgtacaaggg tgagacatct agctctaggc aaccaaccca 28800
tgttaagttg cctaagaaga aaactcctag tgcatcaaat gaacatagca tttcatttaa 28860
gacttttgat gcatcttatg ttttgactaa caaatccggc aaagtagttg ccaagtttgt 28920
tgggggcaaa cacaagggct ccaagacttg tgtttgggta cccaaagttc ttgtttctaa 28980
tgccaaagga cccaaaaccg tttgggtacc taaagtcaag aactaaaatt gttttgtagg 29040
tttatgcatc cggaggctca agttggatac tcgacagcgg gtgcacaaac catatgacag 29100
gggagaagaa gatgttctcc tcctacgaga aaaaccagga tccccaacga gctatcacat 29160
tcggggatgg aaatcaaggt ttggtcaaag gtcttggtaa aatagctata tctcctgacc 29220
attctatttc caatgttttt cttgtagatt cattagatta caatttgctt tctgtatctc 29280
aattatgcaa aatgggctac aactgtcttt tcactgatat aggtgtcact gtctttagaa 29340
gaagtgatga ttcaatagca tttaagggag tgttggaggg tcagctatac ttagtagatt 29400
ttgatagagc tgaactcgac acttgcttaa ttgctaagac taacatgggc tggctctggc 29460
atcgccgact agcacatgtt gggatgaaga atcttcataa gcttctaaag ggagagcaca 29520
ttttaggatt aaccaatgtt cattttgaga atgacagggt ttgtagcgca tgccaggcag 29580
gaaagcaagt tggagcccat catccacaca agaacatcat gacgaccgac aggccgcttg 29640
agctactcca catggatcta ttcggcccga ttgcttacct aagcatcggc gggagtaagt 29700
attgtcttgt gatagtggat gattattctc gcttcacttg ggtgttcttt ttgcaggaaa 29760
aatctcaaac ccaagagacc ttaaaaggat tcttgagacg ggctcaaaat gagttcgcct 29820
taaggatcaa gaaaataaga agcgacaacg gaacggagtt caagaactct caaattgaag 29880
gcttccttga ggaggagggc atcaagcatg agttctcttc tccctacacg tcacaacaaa 29940
atggtgtagt agagaggaag aatcgaactc tattggacat ggcaagaacc atgcttgatg 30000
agtacaagac tttggatcgg ttttgggctg aggcggtcaa caccgcctgc tacgccatca 30060
accggttata tctacaccga atcctcaaga agacatctta tgaactccta accggtaaaa 30120
agcccaatat ttcatatttt agagtctttg gtagcaaatg ttttattctt gttaaaagag 30180
gtagaaaatc taaatttgct cctaagactg tagaaggctt tttactagga tatgattcaa 30240
acacaagggc atatagagtc tttaacaagt ccactggaca agttgaagtt tcttgtgacg 30300
ttgtgtttga tgagactaac ggctctcaag tagagcaagt tgatcttgat gaaataggta 30360
atgaagaggc tccatgcatc gcgctaagga acatgtccat tggggatgtg tgtcctaagg 30420
aatccgaaga gcctccaaat gcacaagatc aactatcctc ctccacgcaa gcatctccac 30480
cgactcaaaa tgaggatgaa gctcaagttg atgaagtaga agatcaagca aatgagacac 30540
ctcaagatga cgacaatgat caagggggag atgcaaatga tcaagacaag gaggatgaag 30600
agcataggcc gccacaccca agagtccacc aagcaatcca acgagatcac cccgtcgaca 30660
ccatcctcgg cgacattcat aagggggtaa ctactagatc tcgtattgca catttttgtg 30720
agcattactc ttttgtttcc tctattgagc cacacagggt agaggaagca ctccaagatt 30780
cggattgggt ggtggcgatg caagaggagc tcaacaactt cactaggaat gaggtatggc 30840
atttagttcc acgtcctaat caaaatgttg taggaaccaa atgggtcttc cgcaacaagc 30900
aagatgagca tggtgtggtg acaaggaaca aagctcgact tgtggccaaa ggatactccc 30960
aagtcgaagg tttggatttc ggtgaaacct atgcacccgt agctaggctt gagtcaattc 31020
gtatattatt ggcctatgat acttaccatg gctttaagct ttatcaaatg gacgtgaaaa 31080
gtgccttcct caatggacca atcaaggaag aggtctatgt tgagcaacct cccggctttg 31140
aagacagtga gtaccctaac catgtctata agctctctaa ggcgctttat gggctcaagc 31200
aagccccaag agcatggtat gaatgcctta gagatttcct tattgctaat ggcttcaaag 31260
tcggaaaagc cgatcctaca ctctttacta aaactcttga aaatgacttg tttatatgcc 31320
aaatttatgt tgatgatatt atatttggat ctactaacga gtccacttgt gaagagttta 31380
gtaggatcat gacacagaaa ttcgagatgt ctatgatggg ggagttgaag tattttctag 31440
gattccaagt caagcaactc caagagggca ccttcattag ccaaacaaaa tacactcaag 31500
atattctaag caagtttgga atgaaggatg ccaagcccat caagacaccc atgggaacta 31560
atgggcatct cgacctcgac acgggaggta agtccgtgga tcaaaagcta taccggtcga 31620
tgataggttc tttactctat ttatgtgcat ctcgaccgga cattatgctt tccgtatgca 31680
tgtgtgcaag attccaagcc gaccctaagg aagcccacct tacggccgta aaacgaatct 31740
tgagatatct ggcttatact cctaagtttg ggctttggta tcctagggga tccacatttg 31800
atttgattgg ttattcggat gccgattggg cagggtgcaa aatcaatagg aagagcacat 31860
ccgggacttg ccagttcttg ggaagatcct tgggtgtctt gggcttcaaa gaagcaaaat 31920
tcggtcgctc tttccaccgc cgaagccgag tatattgccc gcaggccact gttgcgcgca 31980
actgctttgg atgaggcaaa ccctgcggga ctatggttac aaactaacca aggtcccttt 32040
gctatgtgat aatgagagtg caatcaaaat ggtcgacaat cccgtcgagc atagccgcac 32100
taagcacata gccattcggt atcacttttt gagggatcac caacaaaagg gagatatcga 32160
gatttcatac attaatacta acgatcaatt agctgatatc tttaccaagc ctcttgatga 32220
acaatctttt aacaaactta ggcatgagct caatattctt gattctagga acttcttttg 32280
ttaaattgca cacattgttc ttttatatac ctttgatcat atctctttta tatgctatga 32340
ctaatgtgtt ttcaagtcta tttcaaacca agtcataggt atattgaaag ggaattggag 32400
tcttcggcga agacaaaggc ttccactccg tacctcatcc ttcgccatca cttcaagcaa 32460
ctctccgttc tcgggggaga taagcatgag catcaaagaa aaggactttg ggggagaaat 32520
gagcccaaag ccaaaggacc ggacttcgtc tttggtataa tcttaactca tttatttatg 32580
accaaaaggg aaaatagcac ttcgagggct ctaatgattc cgtttttggc gattcatgcc 32640
aaaaaggggg agaaatgagc ccaaagcaaa aggaccgcac caccaccaat ttcaaaaact 32700
tagtgttgaa tatttttcaa tttgtatctt attttcaatt ggtatcttat tgtgttcaaa 32760
agggggagaa agtagtattt taaaatgata tatcaaaaac cctcttgaat actaagagga 32820
ggatctcttt tagggggagt tttgtttaag tcaaaggaaa agcatttgaa acagggggag 32880
aaaatttcaa atcttgagaa tgctttgcaa aaatcctatt catttacctt tgactatttg 32940
caaaagaact ttgaaaagga tttacaaaat aatttgcaaa aacaaaactc gtggtgcaag 33000
cgtggtccaa aatgttatat aaagaaagaa acaatccatg catatcttgt aagtattcat 33060
attggctcaa ttccaagcaa cctttacact tacattatgc aaactagttc aattatacac 33120
ttctatattt gctttggttt gtgttggcat caatcaccaa aaagggggag attgaaaggg 33180
aattaggctt acacctagtc cctaattaat tttggtggtt gaattgccca acacaaacaa 33240
ttggactaac taagtttgca caagtttata gattacacag gtgtaaaagg ttcacactca 33300
gccaataaaa ggaccaagtt tttggattca acaaaggagc atagtgggaa ccgaaggccc 33360
tctggtctga gagcaccgga ctgtccggtg tacaccggac agtgtccggt gcaccagagg 33420
actcaagctc caactcgccc ccttcgggaa ttgccagagg cgactccgct ataattcacc 33480
ggactgtccg gtgtacaccg gacagtgtcc ggtgcgccaa gggaggtcgg cctcaggaac 33540
tcgccagctt cgggaaaagc catcggcccg tccactataa ttcaccggac tgtctggtgt 33600
gcaccggact gtccggtgcg actcgagagc aacggctatt tccgcgccaa cggctctctg 33660
ccgcgcattt aatgcgcgct ctgcgcgcgc agatgtcagg cgcgcccact ccggcacacc 33720
ggacaaggaa cagtagatgt ccggtgtgca ccggacaccc aggcgggccc acaagtcaga 33780
agcttcaacg gctagaatcc aacggcagtg atgacgtggc aggggcaccg gactgtccgg 33840
tgtgcaccgg actgtccggt gcgtcatcga acagacaacc cagccaacgg tcaagtttgg 33900
tggttggggc tataaatacc cccaaccacc ccaccattca ttgcatccaa gttttccact 33960
tctcaactac tacaagagct ctagcattca attctagaca caccaaaaga gatcaaatcc 34020
tctccaattc cacaaaaggc tttagtgatt agagagagag atttgccgtg ttctttttga 34080
gctcttgcgc ttggattgct tcttttcttt cgcacttgtt cttgtgatca aaaacccatt 34140
gtaatcaagg caagaggcac caattgtgtg gtggcccttg ttgggaagtt ttgttcccaa 34200
ctttgattag agaagagaag ctcactcggt ccgagggacc gtttgagaga gggaagggtt 34260
gaaagagacc cggcctttgt ggcctcctca acggggagta ggtttgcgag aaccgaacct 34320
cggtaaaaca aatccacgtg tcacactctt catttgcttg cgatttgttt tgcgccctct 34380
ctcgcggact cgtttcttca ttactaacgc taacccggct tgtagttgtg tttatatttg 34440
taaatttcag tttcgcccta ttcacccccc ctctaggcga ctatcaaaaa cagtgcaacc 34500
atgagggtgg aatggggtgc ccttagctga ataattagag gatccggggt gtagttcact 34560
tagccatcgt gccgtcaatg gggctcggtg tatgcggctc gctctgccaa gtttgggttc 34620
gccccttggg gaggagtgcg gtgcatttag gaaacctaac gggtggctac agtcccgggg 34680
aatctttgta aaggctacgt agtgatgccc tgctgggtca ccttggtagt gatcaatgga 34740
gagtcatgat ctccgggcag aatgggaatc acggcttgtg ggtaaagtgc acaacctctg 34800
cagagtgttt gaaaactgat atatcagccg tgctcacggt tatgagcagc caagggagct 34860
ccagtgatta gtggtacttg atcagagata ctttggtaca ggtggttatg agatcgatga 34920
ttctggttat gactatgatg ctggtaagtg gtactctttc cgtttggaaa ggagtacgtt 34980
tgggttaata acttgggtta atgctaaaac ttggctttct attagtaaat aataatctga 35040
ccaactaaaa gcaactgctt gacttatccc cacataaagc tagtccacta cagccaaaca 35100
ggatacttgc tgagtatgtt gatgtgtact cacccttgct ctacacacca aacccccccc 35160
ccatccccag gttgtcagca ttgcaaccac tgctcagtcg aagatgaagc tgtggaagga 35220
gacttccagg agttccaaga ttacgacgag ttctaggtgt gggttagcgg caacccccag 35280
tcggctgcct gtgaaggccg cggttatcta cgtttctttt ccgcactttg atttattgta 35340
agaactatat ggacgtctca gacgtatgat gtaatcgact atttccctta gtaatactat 35400
tttgagcact gtgtgatgat gtccatgtta tgtaactgct gtgtacgtga ataactgatc 35460
ctggcacgta catggttcgc attcggtttg ccttctaaaa ccgggtgtga cacctgatta 35520
ctctcaagca aagcctatag gtagtttaag aggttgagta caatgagaaa catttcaatc 35580
attatttgca aaagaaacat tttgatcata ttaaggaaaa tcataggagt gaaagaaaaa 35640
caatgtgtgc aaataactga acctcctgca gctccatcat gctggccaaa gtatgcttcg 35700
acgaattcaa aatatcatca tatgcttgct ctatctgctt atcatgattg caaccttgtt 35760
cggatgggtt cctggcttcc tgcaacagca cagtagaaaa caaactggag tttagaattc 35820
aacttgagac ttcagagttg aaacaaaacc tgtattcaca tatgtagcac cgatgcatat 35880
acaaatacct ttatcagaaa catgaagata tgtgaataca ttattttctc aataaaagtc 35940
atgatagaat tcatgcaaat ttttttagaa ataaataaat gatgaggcat actacaattc 36000
taaaagcagt agcagtgcaa cacgaacgaa cattcaaatt gccccacatt cttgaaactg 36060
tgctgctttg ctctcgttcc aaaaaaactc cgctaaagta aaacttggaa aggtctggtt 36120
ttgcgtgagc caccaacacc aaccaaactt tacgtcacta tgacagtttc agcctttcgg 36180
tcccggcgac agccatggcg gacgcggggg tgacaggggg tgctggccaa gctgggtgag 36240
ctgacggagg aggaggcgac gacgctgctg cgcgtggacg ccgagatacg ggcattgtgg 36300
cagaagctgg cctacctgca ggcgctcgta cgcggggccg gccgccagcg ccgcgaccgc 36360
gcaagcgagc tgctcctgct ctggctacgc gagaccagag aggttgcttt cgcgggtggt 36420
tctgccatac atagcggcga tggctcttcc tcccaggact accatatcta cacataacaa 36480
tcatgctctc atgaagttgt gatgtaatag gtcacacgat tttaatgtat aagattgtga 36540
agagtaaatt aattcaaatg aattcatgga catgggacaa ctatgtgtta aaaacagaat 36600
ctcctatgta tcctaaccat gtgtaatgac atgacaaaat gacacttgta tatgagcaat 36660
taaagcatca tactaccttg tagagttctc ttcggcgaat acgttgaatg atttcttttg 36720
ctttcttcag tttattgtta ggagcagtct cgtagacaca gtcaagtcct agcattagct 36780
cactgcgaga gtatcagcca ttccagactc caggagctcc tcccgggtag cctgcctggc 36840
aatgggcgca gatatcctct tgagctcact cttaatgcta ggttgtaggc tccttgcttc 36900
caggtccatc ttccgaatct gcagacacaa aacaaaaaca taaaattact tttcccccaa 36960
cccccaaatt gaagactaag gcacataaac atctcgcaac tccgttggaa caccaaaact 37020
gcaccgacgg aaacgaaaaa tccctaatct ctgcttttct acccattccc caaatgtcgt 37080
atgtcccgtt agccaagggg atccccaaaa cagaacgtgt cacgacatct atttaccagc 37140
gattcagctt cctcaacgcc ggattggatc tcagagagct tctgcttctt cctctctgcg 37200
gacagaccca ccgaaatcag accaaaacaa acggtcaaca aaagaaggct ttccaagcgg 37260
cgctactgac gctcggctgc ctggccggcg agcctcgcag tcggccctcg atgaaggagg 37320
tggtggagac gctggagcgg gtggaggcga tgaagagccg ggcacgcggc gcgcgtacac 37380
cgcccggttc ctgccccccc ccccccccga cgacgaccat ggcgcttcgc cgatggccca 37440
cttgcctggt cgccccgccg tcgcgtcagc gagcgcgctc agcagctccg tgacccgctc 37500
gtcgaaccgc gtggccacgg cgagcggcac gaggcctggg ttcgtgacgt tgaccggggg 37560
gcggcggggg tgaaagggaa ttaggctcac acctatttcc taattgattt tggtggttga 37620
attgtctaac acaaataatt ggactaacta gtttgctcta gtctataagt tttacaggtg 37680
ccaaaggttc ataataagcc aataaaaaga ccaagaaagg gttcaaacaa aaagagcaaa 37740
agacatcccg gaaggcaccc tggtctggcg caccggactg tccggtgtgc caccggacag 37800
tgtccggtgc accagggcac tcgaagctga actcgctacc ttcgggaaaa tcagagggcg 37860
ctccgctata attcaccaga ctgtccggtg aagcaccgga ctgtccggtg tgccagcgga 37920
gcaacggcta cttcgcgcgc aacggtcgac tgcaacgcat tcaatgcgcg cctgcgcgcg 37980
cagagggcag agcactcaca gttggcgcac cggacagtct acaggacctg tccggtgcac 38040
caccggacag cccagaggcc ccacaagtca gagctccaac gatcgaaccc caacgatctg 38100
ctgacgtggc tggcgcaccg gactgtccgg tgcgccatgc gaccgcagcc ttccaacggc 38160
catttttggt ggtttagggc tataaatacc ccaaccaccc cacattcaat ggcatccaag 38220
tttcccacct tcaacacatt acaagagcta taacattcaa ttctagacac tccaaaagat 38280
caaatcctct cccaagtccg gaatcactcc aaatcaaata gtgactagag agagcgacat 38340
ttgtgttcat ttgagctctt gcgcttggat cgcttctttt ctttctcatt cttcttgtga 38400
tcaaactcaa ttgtaaccaa ggcaagagac accaattgtg tggtggtcct tgcaggaact 38460
ttgtgttccg tttgattgag aagagaagct cactcggtct aagtgaccgt ttgagagagg 38520
gaaagggttg aaagagaccc ggtctttgtg accacctcaa tggggagtag gtttgcaaga 38580
accgaacctc ggtaaaacaa atcatcgtgt ctcgctcttt atatttctaa cgttaacccg 38640
gcttgtagtt gtgcttaagt ttgtaaattt cagattcgcc ctattcaccc cccctctagg 38700
cgactttcaa ttggtatcgg agccggtgct tcattagagc ctaactgctc gaagtgatgt 38760
cgggagcatc cgccatgagg gatctcggga ccggcgacaa gaccgcatgc tcgggaagaa 38820
ctcactcaag ggagtccgcc cacaagcata aggaggaatc gtcttcctcc atcaagtccc 38880
atcggatggg tgacaaaaag aagaagatga ggaaggtggt ctactacgag accgactctt 38940
cgtcaccctc cacctccggc tcggaatcgg cctccaccac ttcaaagcgc catgagcgca 39000
agaagtatag taagatgccc cttcgctatc ctcgcatttc tagacgcact ccatcactct 39060
tcgttccatt aggcaaacca cctatatttg aaggtgaaga ttattctatg tggagtgata 39120
aaatgaggca tcacctaacc tcactccaca aaagcatatg ggatattgtt gagtatggag 39180
tgcaggtacc aaagaaggga gataaagatt acgactcgga ggaggttgaa caaatccaac 39240
atttcaaatc caagtcgaga ggagtataat aaggtgcaag ggttgaagag tgcaaaggat 39300
atctgggacg tgctaaagac cgcgcacgaa ggagacgagg taaccaagat caccaagcgg 39360
gagacgatcg agggggagct cggtcgcttc cggcttcgcc aaggggagga gccacaagat 39420
atgtacaacc ggctcaagac cttggtgaac caagtgcgca acctcgggag caaaaaatgg 39480
gatgaccatg aaatggttaa ggttattctt agatcacttg tgttccttaa ccctacgcaa 39540
gttcaattaa ttcgtggtaa tcctagatat acactaatga ctcccgagga agtaatagga 39600
aactttgtga gctttgagtt gatgatcaaa ggctcaaaga aaattatcga gcacgacggt 39660
ccctccacgc ccgaagcaca accggtcgca ttcaaggcga cagaggagaa gaaagaggag 39720
tctacatcaa gtagacaacc catcgacgcc tctaagctcg acaacgagaa aatggcgctc 39780
atcatcaaga gcttccacca aatcctcaaa caaaggaagg ggaaagatta caagccttgt 39840
tccaaaaggg tgtgctacaa gtgtggtaag cccggtcatt tcattgttaa atgtccttta 39900
tctagtgata gtgacagggg cgacgacaag aagggcaaga ggagagaaaa gaggaggtat 39960
tacaagaaga agggcggcga tgcccatgtg tgccgcgagt gggactccga cgagagttcc 40020
tccgactcct catccgacga ggacgccgcc aacatcgccg tcaccaaagg gctcctcttc 40080
cccaacgtcg gccacaagtg cctcatggca aaggacggca aaaagaagaa ggtaaaatca 40140
aaatcctcca ctaaatatgc atcctctagt gatgaagata atgctagtga tgaggaggat 40200
aatttgcgta ccctttttgt caacctaaac atgcaactac aggaaaaact aaatgaatta 40260
attagtgcta ttcatgagaa agatgatctc ttggactttc aagaggactt cctaattaag 40320
gaaaataaga agcatgttaa ggttaaaaat gcttatgctc tagaagtaga aaaatgtgaa 40380
aaattatcta gtgagctaag cacttgccat gatactatta ccatccttag aaataaaaat 40440
actaaactaa ttgctaaggt tgattctaat atttgtgatg tttcaattcc caatcttaga 40500
gatgataatg ttaatttgct tgctaagatt gaagaattga atgtctctct tgctagcctt 40560
agggttgaaa atgaaaaatt gattgctaag gctaaagaat tagatgtttg caatgcttcc 40620
atttctgatc ttagaaataa caatgatatt ttacgtgcta agattgttga acttaattct 40680
tgcaaaccct ctacatctgc cattgagcat gtcattattt gcactagatg tagagatatt 40740
aacattgatg ctattcatga tcatatggct ttaattaaac aacaaaataa tcatatagca 40800
aaattagatg ctaaaattgc cgagcatgac ttaaaaaatg aaaaatttaa atttgctaga 40860
agcatgctct atagtgggag acgccctggc atcaaggatg gcattggctt ccaaaaggga 40920
aacaatgtca aacttaatgc ctctcctaaa agattgtcaa actttgttaa gggcaaggct 40980
cccatgcctc aggataatga gggttacatt ttgtaccctg ccggttatcc cgagagcaaa 41040
attaggagaa ttcattctag gaagtctcac tctggccata atcatgcttt tatgtataag 41100
ggtgagacat ctagctctag gcaatcaacc cgtgcaaaat tgcctaagaa gaaaactcct 41160
gctgcatcaa atgatcataa catttcattc aaaacttttg atgcatctta tgttttaact 41220
aacaaatccg acaagatagt tgccaagtat gttgggggca aacacaaggg atcaaagact 41280
tgtgtttggg tacccaaagt tcttgtatct aatgtcaaag gacccaaaac catttgggta 41340
cctaaaatca agaactaaac ttgttttgta ggtttatgca tccgggggcc caagttggat 41400
catcgatagc gggtgcacaa accatatgac aggggagaag aaaatgttct cctcctatga 41460
gaaaaaccaa gatccccaaa gagcgatcac attcggggat ggaaaccaag gtttggtcaa 41520
aggattgggt aaaattgcta tatctcctga ccattccatt tccaatgtgt ttcttgtaga 41580
ttctttagat tacaacttgc tttcagtttc gcaattatgt aaaatgggct acaactgtct 41640
ttttacagat ataggtgtta ctgtctttag aagaagtgat gattcagtag catttaaggg 41700
agtgttagag ggtcagctat acttggtaga ttttgataga gctgaactcg acacttgctt 41760
aattgctaag actaacatgg gctggctctg gcatcaccga ctagcacatg ttggaatgaa 41820
gaatcttcac aagcttctaa agggagaaca cattttggga ctaacaaatg ttcactttga 41880
gaaagatagg atttgtagcg catgtcagac agggaagcaa gttggtactc atcatccaca 41940
caagaacatc atgatgactg acaggccact cgagctccta catatggacc tattcggccc 42000
gatagcttac ataagcatcg gcgggagtaa gtactatcta gttattgtgg atgattatac 42060
tcgcttcact tgggtattct ttttgcagga aaaatctcat acccaagaga ccttaaaggg 42120
attcttggga cgggctcaaa atgagttcgg cttaagaatc aaatttgttt taagcgacaa 42180
cgggacggag tcaagaatct caaatcgaag gcacgatctc ctagatccgg cccaaaannn 42240
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 42300
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc tgatgaatca gcttgattcg 42360
tgacacggga ggtaagtccg tggatcaaaa ggtataccgg taagataata ggctctttac 42420
tctatttatg tgcatctcga ccggatatta tgctttccgt atgcatgtgt gcaagattcc 42480
aagctgaccc taaggaagct caccttacgg ccgtaaaacg aatcttgaga tatttggctt 42540
atactcctaa gtttgggctt tggtatccta ggggatccac atttgatttg attggttatt 42600
cggatgctga ttgggcgggg tgtaaaatca atagaaagag cacatcaggg acttgccagt 42660
tcttgggaag atccttggtg tcttgggctt caaagaagca aaattcggtc gctctttcca 42720
ccgccgaagc cgagtacatt gccgcaggcc attgttgcgc gcaattgctt tggatgaggc 42780
aaaccctgcg ggactatggt tacaaattaa cctaagtccc tttgctatgt gataatgaga 42840
gtgcaatcaa gatggcggat aatcccgtcg aacatagccg cactaaacac atagccattc 42900
ggtatcattt tcttagggat caccaacaaa agggagatat cgagatttct tacattaaca 42960
ctaaagatca attagccgat atctttacca agcctcttga tgaacaaacc tttaacaaac 43020
ttaggcatga gctcaatatt cttgattcgc gcaatttctt ttgctaaatt gcacacatag 43080
ctcatttata tacctttgat catatctctt tcatatgcta tgactaatat gttcttcaag 43140
tctatttcaa accaagtcat aggtgtattg aaagggaatt ggagtcttcg gcaaagacaa 43200
aggcttccac tccgtaactc atccttcgtc gtcgctctgg gccactctcc atctttgggg 43260
gagagagcaa aagacttcgt ctttggtaca atcttaactc atttatttat gaccaaaggg 43320
gaagaaagta cttcgagggc tctaatgatt ccgtttttgg cgattcatgc caaaggggga 43380
gagagtatga gcccaaagca aacggaccgc accaccacca atttcaaaaa cttagttttt 43440
caaagagtat tttcaattgg tatcctattg tgttcaaaag ggggagaaag tagtattttc 43500
aaaaatgata tatcaaaacc ctcttgaaca ctaagaggtg gatctcattt agggggagtt 43560
ttgtttagtc aaaggaaaag catttgaaac agggggagaa aatttcaaat cttgaaaatg 43620
cttcataaaa tcgtattcat ttacctttga ctttttgcaa aagaactttg aaaaggattt 43680
acaaaatagt ttgcaaaaac aaaacatgtg gtgcaagtgt ggtccaaaat gataaaaaca 43740
aaggaacgat ccatgcatat cttgtaagta tttatattgg ctcaaatcca agcaaccttt 43800
gcacttacat tatgcaaact agttcaatta tgcattttat acttgctttg gtttgtgttg 43860
gcatcaatca ccaaaaaggg ggagattgaa agggaattag gcttacacct agttcctaaa 43920
taattttggt ggttgaattg cccaacacaa ataattggac taactagttt gctctagtgt 43980
acaagttata caggtgccaa ggttcacaac aagccaatta aaaagaccaa agttgggttc 44040
aaaatagaga gccaaaggca tcccgaaagg ctccctggtt tggcgcaccg gactgtccgg 44100
tggcgcaccg gacagtgtcc ggtgcaccag gggacctcgc gcagaactcc tcagcctcgg 44160
gaatttttcg gagccgccgc gctataattc accggactgt ccggtgtaca ccggacagtg 44220
tccggtgctc caagaaaaca cggctccaga acttggcagc ctcgggaaat cagaacggct 44280
gctccgctat aattcaccgg acatgtccgg tgtacaccgg actgtccggt gcaactgcgg 44340
agcaacggct acttcgcgcc aacggtcacc tgcaggcgca ttcaatgcgc gccagaagcg 44400
cgcagaagtc aggcacgccc atgctggcgc accggacact ctacagtaca tgtccggtgc 44460
gccaccggac atcaaggcgg gcccagaagg cagaactcca acggtcaaat tccaacggct 44520
ttggtgacat ggctggcgca ccggactgtc cggtgcacca tacgacagac agcctccacc 44580
aacggtcatg tttggtggtt ggggctataa ataccccaac caccccacca ttcattgcat 44640
ccaagttttc cagcttccaa ccactataca agagctagca ttcattgcaa agcacaccaa 44700
aagagatcaa atcctctccc aactccacac aaagccttag tgattagaga gagtgatttg 44760
tagtgttcat ttgagctctt gcgcttggat cgcttctttt ctttggcatt ctttcttgtg 44820
atcaaacact cacttgtaat tgaggcaaga gacaccaatt gtgtggtggt ccttgcgggg 44880
agtttgattc ccaagtgatt tgagaagaga agctcactcg gtccaaggga ccgtttgaga 44940
gagggaaggg ttgaaagaga cccggccttt gtggcctcct caacggggag taggtttgag 45000
agaaccgaac ctcggtaaaa caaatccacg cgtctcactt cattattcgc ttgcgatttg 45060
ttttcacgcc ctctctcgga ctcgttctta tttctaacgc taacccggct tgtagttgtg 45120
tttatatttg taaatttcag tttcgcccta ttcacccccc ctctaggcga ctatcaaata 45180
gccagtgctt tttggtctgc gagttcctgc acttggttaa tcaactgtgt cgcttgatct 45240
tctacttgtt tgcacgagaa ggtcaaagcc actttcgaag ctattagttc agaacacaca 45300
acatctagct aaatacatca ccagtttgaa gtcattgatt gtattcttga tatcatcttt 45360
attcttgaat gtcatttgtg ccagttcatt taactcttgt gctgcaaacc aacctgacat 45420
cgtcaattca tttaatctct caatctcagt ttcctttttt tgtttcacat tgaagctccc 45480
taattgttgc ttctcgatgt gcagtggcct gattagctac aagaagctct tgttccatgg 45540
actcgatctc tggaccgcac tatcgttgcc tcagccccta ggtcgtgctg ccctctggcc 45600
tcctcatcgt acaattcacc aacatctcca atgtaagtgc agcaggttca gtaatgaact 45660
cagaagtggc atcagaatac tccaagagtt ttttgatctt tttgcctgga tatataccaa 45720
gggaaatgca ttcaaaactc ctatagatga cgaatcccat ctctccctct tttctcggac 45780
acggatcccc aggtccgtct ccgtgcttta ctcatttgtt ttttacaagt tcagatccac 45840
ttgcgtactc acacggtgga catctgttat gcacatgtgt aaaccagcat aagtccttac 45900
actcgaaaat gcatgtgtta tttagcttga gaataaataa aattattagc aaggagaaaa 45960
caaaaaaata ggactaaaca atagagtcac attggtttaa attagtacct agaagtaaaa 46020
aaagatgatc taaattagat acatcatacc aaataccata ttactattcc agttaccccg 46080
tctactatgc ctagatatca aattcttgaa ggttggcctt ctcattttca gtaatagcct 46140
gacgaaagta gagtatgttt gtatgagcaa ttatgctgct cactactgcc ttgcgctata 46200
ataggccact actgatttta catgcttttt ctacattaga tagctcacaa acatgctacc 46260
tcaaaaaaat gatggcaaag gggagccaca aaatgtcaat tattttgtca agtattagca 46320
gttttcttgt gtatgtgatc agactaacac tgcatgtctt tgttttcctg taaaactatg 46380
tatgatgaaa ccatggtgtg attgtattgg ctggccttac cctgttttgt tgcaatgcat 46440
tcgttgttgt acaggtaata tgttgaaaca caattcattg catatgacaa ttctgttttt 46500
tctttctaga atattgacat attgtttgat cattattttc taagcaataa tcatggctat 46560
tcttatagta ttgcataata cctttttctt ttcgaaccct agcgcattga ttctttagtg 46620
aagtgattat agtgattcca gcgggagagt agggtgggga gcagagggtt gattctggac 46680
tgatttcggt ggagattaaa tggggagcag tgaggagcat gtttttttag atcccaccag 46740
aatatgtgcg ccattttgct atttggctga ggagtgatgc tcagggagaa tccgttctca 46800
ggagctgtgc caaatgtcgc cttaggtttt atgatatgac ctgacttctg tgttaatatt 46860
tgttagatct ttattttatt tgaggttaca aaggtggtgt tctcaagcta gaaacaaagt 46920
tgtggctagg tcaaaactag atgatgctct tgaaccgtgt cttttgactc tgttacttgt 46980
tgcaggtttg atgttcacta atatgttgtt caactttgag caggtcagca attgactggt 47040
gttgctggta gcctggcata tctggcacct gaggttctac taggaaatta ctcccaaaag 47100
gtagatgtat gggctgccgg ggtgcttctg catgttctgt tgatgggcac tcttccgttc 47160
caaggaaaat ctatcgaagc tatctttgat gttataaaga ctgctgaact tgactttcac 47220
aatagtcagt gggcatctgt gtcacttctt gcttatgatc tcattggtcg aatgcttaat 47280
cgagaggtct cttcaaggcc cgatgccgaa gatgttctcc gtaagttcaa gcacccttgt 47340
aacttgtgct ttatatatat atataatata tatatatata tgattctcaa tttatcattg 47400
acttttccta atggctttca acacagggca cccatgggtc ttattctaca ctgattgcct 47460
gcagaaagct gaattctcta acctatggga tactaacaaa actgcagctc ccatgattca 47520
tcgggagata gtcaggtttg gttactgcga gtcttcatct tcaaaatcct caagtgacaa 47580
ctctgaagag cgagatgaat gcggtatagt tgatgcactg gcgacaacaa taacacaggt 47640
gaggatctca gagcccaaga ggagtcggct gttcagccta cccaacgggt tgttgccgcc 47700
aagcaggaac agtctccgaa catgaagatg atgaatccgt gtgtggcttt ctaacttgac 47760
ctacctagct cccatcccca tgcatgtata aacgagataa acgagctctg tgattttata 47820
gatggaaaat tttcaccgtg gttgatgttt tgcgattgct agctcgctga gcctgcaatc 47880
ctctgtaaat atatcattgt tgtcatcatt tttgtacatc gatgacaccg taattgattc 47940
gatt 47944
<210> 2
<211> 1524
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 2
atggggagca gtgaggagca tgttttttta gatcccacca gaatatgtgc atccgtgtca 60
cttcttgctc atgatctcat tggccgaatg cttaatcgag aggtctcttc aaggcccaat 120
gccaaagaag ttctccctcc catgattcat cgggagatag tcaggtttgg ttactgtgag 180
tcttcatctt caaaatcctc aagtgacaac tctgaagagc gagatgaatg cggtatagtt 240
gatgcactgg tgacaacaat aacacagatt cggaagatgg acttggaggc aaggagccta 300
cagcctagca ttaaggctgg tttgcttgca aagctgaggg agtataaatc tgacctcaac 360
aacgtcaaga tgggtctatc cgcagagagg aagaagcaga agctctccga gatccaatcc 420
ggcgttgagg aagctgaatc gctgattcag aaaatggacc tggaggcaag gagcctacag 480
cctagcatta aggctggttt gcttgcaaag ccgagggatt ataaatctga cctcaacaac 540
gtcaagagtg agctcaagag gatatctgcg cccaatgcca gtggcctgat tagctacaag 600
aagctcttgt tccatggact cgatctctgg accgcactat cgttgcctca gcccctaggt 660
cgtgctgccc tctggcctcc tcatcgtaca attcaccaac atctccaatg tcagcaattg 720
actggtgttg ctggtagcct ggcatatctg gcacctgagg ttctactagg aaattactcc 780
caaaaggtag atgtatgggc tgccggggtg cttctgcatg ttctgttgat gggcactctt 840
ccgttccaag gaaaatctat cgaagctatc tttgatgtta taaagactgc tgaacttgac 900
tttcacaata gtcagtgggc atctgtgtca cttcttgctt atgatctcat tggtcgaatg 960
cttaatcgag aggtctcttc aaggcccgat gccgaagatg ttctccggca cccatgggtc 1020
ttattctaca ctgattgcct gcagaaagct gaattctcta acctatggga tactaacaaa 1080
actgcagctc ccatgattca tcgggagata gtcaggtttg gttactgcga gtcttcatct 1140
tcaaaatcct caagtgacaa ctctgaagag cgagatgaat gcggtatagt tgatgcactg 1200
gcgacaacaa taacacaggt gaggatctca gagcccaaga ggagtcggct gttcagccta 1260
cccaacgggt tgttgccgcc aagcaggaac agtctccgaa catgaagatg atgaatccgt 1320
gtgtggcttt ctaacttgac ctacctagct cccatcccca tgcatgtata aacgagataa 1380
acgagctctg tgattttata gatggaaaat tttcaccgtg gttgatgttt tgcgattgct 1440
agctcgctga gcctgcaatc ctctgtaaat atatcattgt tgtcatcatt tttgtacatc 1500
gatgacaccg taattgattc gatt 1524
<210> 3
<211> 1082
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 3
cctgcccttc cattcttccc ccgctgcccc cggtcaacgt cacgaacccg ggcctcgtgc 60
cgctcgtcgt ggccacactg ttcgacgagc gagtcacaga gctgctgagc gtgctcgctg 120
atgcggcggt ggggcgacca ggcaggtggt ccatcggcga agcgccatgg tcgtcgtcgg 180
ggggcacgaa ccaggcggtg tacgcgcgcc gcgcgcccgg ctcttcatcg cctccacccg 240
ctccagcgtc tccaccactt ccttcatcga gggccgactg cttggctcgc tggccaggca 300
gccgagcatt agttgcgccg cttggaacgc ctgcttttgt tgatcgtttg ttttggtctg 360
atttcagtgg gtctatccgc agagaggaag aagcagaagc tctccgagat ccaatccggc 420
gttgaggaag ctgaatcgct gattcagaaa atggacctgg aggcaaggag cctacagcct 480
agcattaagg ctggtttgct tgcaaagccg agggattata aatctgacct caacaacgtc 540
aagagtgagc tcaagaggat atctgcgccc aatgccagat tcggaagatg gacctggaag 600
caaggagcct acaacctagc attaagagtg agctcaagag gatatctgcg cccattgcca 660
ggcaggctac ccgggaggag ctcctggagt ctggaatggc tgatactctc gcagtgagct 720
aatgctagga cttgactgtg tctacgagac tgctcctaac aataaactga agaaagcaaa 780
agaaatcatt caacgtattc gccgaagaga actctacaag atatggtagt cctgggagga 840
agagccatcg ccgctatgta tggcagaacc acccgcgaaa gcaacctctc tggtctcgcg 900
tagccagagc aggagcagct cgcttgcgcg gtcgcggcgc tggcggccgg ccccgcgtac 960
gagcgcctgc aggaagccag gaacccatcc gaacaaggtt gcaatcatga taagcagata 1020
gagcaagcat atgatgatat tttgaattcg tcgaagcata ctttggccag catgatggag 1080
ct 1082
<210> 4
<211> 2321
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 4
tgaggagcat gtttttttag atcccaccag aatatgtgca tccgtgtcac ttcttgctca 60
tgatctcatt ggccgaatgc ttaatcgaga ggtctcttca aggcccaatg ccaaagaagt 120
tctccgtaag ttcaagcacc cttgtaactt gtgctttata tatatgattc tcaatttatc 180
attgactttt cctaatggct ttcaacacag ggcaccatgg gtcttattct acactgattg 240
cccgcagaaa gctgaattct ctaacatatg ggatactaac aaaactgcag ctcccatgat 300
tcatcgggag atagtcaggt ttggttactg tgagtcttca tcttcaaaat cctcaagtga 360
caactctgaa gagcgagatg aatgcggtat agttgatgca ctggtgacaa caataacaca 420
ggtgaggatc tcagagccca agaggagtcg gctgttcagc ctacccaacg ggttgttgcc 480
gccaagcagg aacagtctcc gaacatgaag atgatgaatc cgtgtgtggc tttctaactt 540
gacctaccta gctcccatcc ccatgcatgt ataaacgaca tttggggaat gggtagaaaa 600
gcagagatta gggattttcg tttccgtcgg tgcagttttg gtgttccaat ggagttgcga 660
gatgtttatg tgccttagtc ttcaatttgg gggttggggg aaaagtaatt ttatgttttt 720
gttttgtgtc tgcagattcg gaagatggac ttggaggcaa ggagcctaca gcctagcatt 780
aaggctggtt tgcttgcaaa gctgagggag tataaatctg acctcaacaa cgtcaagagt 840
gagctcaaga ggatatttgc gcccaatgcc aggcaggcta cccgggagga gctcctagag 900
tttggaatgg ctgatactct cgctgtgagc taatgctagg acttgactgt gtctacgaga 960
ctgctcctaa caataaactg aagaaagcaa aagaaatcat tcaacgtatt cgccgaagag 1020
aactctacaa ggtagtatga tgctttaatt gctcatatac aagtgtcatt ttgtcatgtc 1080
attacacatg gttaggatac atacttaagt ttctaacgta ggcgtccaca caacggattg 1140
gtgcacggtt ctgccgatgt atcccacgca cgtgcatgga aggaggcagg cacccttccc 1200
cgccgccccg gatctcgcgc cagcccccgc cctaccccgc ctgcccttcc attcttcccc 1260
cgctgccccc ggtcaacgtc acgaacccgg gcctcgtgcc gctcgtcgtg gccacactgt 1320
tcgacgagcg agtcacagag ctgctgagcg tgctcgctga tgcggcggtg gggcgaccag 1380
gcaggtggtc catcggcgaa gcgccatggt cgtcgtcggg gggcacgaac caggcggtgt 1440
acgcgcgccg cgcgcccggc tcttcatcgc ctccacccgc tccagcgtct ccaccacttc 1500
cttcatcgag ggccgactgc ttggctcgct ggccaggcag ccgagcatta gttgcgccgc 1560
ttggaacgcc tgcttttgtt gatcgtttgt tttggtctga tttcagtggg tctatccgca 1620
gagaggaaga agcagaagct ctccgagatc caatccggcg ttgaggaagc tgaatcgctg 1680
attcagaaaa tggacctgga ggcaaggagc ctacagccta gcattaaggc tggtttgctt 1740
gcaaagccga gggattataa atctgacctc aacaacgtca agagtgagct caagaggata 1800
tctgcgccca atgccagatt cggaagatgg acctggaagc aaggagccta caacctagca 1860
ttaagagtga gctcaagagg atatctgcgc ccattgccag gcaggctacc cgggaggagc 1920
tcctggagtc tggaatggct gatactctcg cagtgagcta atgctaggac ttgactgtgt 1980
ctacgagact gctcctaaca ataaactgaa gaaagcaaaa gaaatcattc aacgtattcg 2040
ccgaagagaa ctctacaaga tatggtagtc ctgggaggaa gagccatcgc cgctatgtat 2100
ggcagaacca cccgcgaaag caacctctct ggtctcgcgt agccagagca ggagcagctc 2160
gcttgcgcgg tcgcggcgct ggcggccggc cccgcgtacg agcgcctgca ggaagccagg 2220
aacccatccg aacaaggttg caatcatgat aagcagatag agcaagcata tgatgatatt 2280
ttgaattcgt cgaagcatac tttggccagc atgatggagc t 2321
<210> 5
<211> 1082
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 5
cctgcccttc cattcttccc ccgctgcccc cggtcaacgt cacgaacccg ggcctcgtgc 60
cgctcgtcgt ggccacactg ttcgacgagc gagtcacaga gctgctgagc gtgctcgctg 120
atgcggcggt ggggcgacca ggcaggtggt ccatcggcga agcgccatgg tcgtcgtcgg 180
ggggcacgaa ccaggcggtg tacgcgcgcc gcgcgcccgg ctcttcatcg cctccacccg 240
ctccagcgtc tccaccactt ccttcatcga gggccgactg cttggctcgc tggccaggca 300
gccgagcatt agttgcgccg cttggaacgc ctgcttttgt tgatcgtttg ttttggtctg 360
atttcagtgg gtctatccgc agagaggaag aagcagaagc tctccgagat ccaatccggc 420
gttgaggaag ctgaatcgct gattcagaaa atggacctgg aggcaaggag cctacagcct 480
agcattaagg ctggtttgct tgcaaagccg agggattata aatctgacct caacaacgtc 540
aagagtgagc tcaagaggat atctgcgccc aatgccagat tcggaagatg gacctggaag 600
caaggagcct acaacctagc attaagagtg agctcaagag gatatctgcg cccattgcca 660
ggcaggctac ccgggaggag ctcctggagt ctggaatggc tgatactctc gcagtgagct 720
aatgctagga cttgactgtg tctacgagac tgctcctaac aataaactga agaaagcaaa 780
agaaatcatt caacgtattc gccgaagaga actctacaag atatggtagt cctgggagga 840
agagccatcg ccgctatgta tggcagaacc acccgcgaaa gcaacctctc tggtctcgcg 900
tagccagagc aggagcagct cgcttgcgcg gtcgcggcgc tggcggccgg ccccgcgtac 960
gagcgcctgc aggaagccag gaacccatcc gaacaaggtt gcaatcatga taagcagata 1020
gagcaagcat atgatgatat tttgaattcg tcgaagcata ctttggccag catgatggag 1080
ct 1082
<210> 6
<211> 3646
<212> DNA
<213> Zea mays
<400> 6
atggcacact ttgatgaact agaggataaa acaacagatt atgttgattt atcggttcaa 60
gaatttgctc ttaagcaacc tcaatgtggc atggcttata attactatgg aaatttaagg 120
ctttatgtag tagccaataa agctgaattg gcctcttcaa tatttgaaat cgataaggta 180
aacaaaggcg gagttaatgc atctatgcca gtgaccactt ccactcctaa ttcgaatcaa 240
aattcatgaa ccggttatgg aacaaataga gaatcaagtt tcggtgaggt ataatacgat 300
tcccctaacc catggaattt acctagtaaa aatcctgtag ttaatagtgt actagtaact 360
tctgtcaccg acttgaataa agctttgaat gagtataaaa atgagatgtc taaatttatt 420
gagaatagct tggtgtatag attaagccta gtagaaacac ttataacaag ttgtatgctt 480
caattttttt tgattttttt ggaagctact catagttgga gggtaccaaa tttacaaaaa 540
aaaattggtg attataatag taaatctacc atagaacatg ttagcttgtt tcttgctctg 600
agaggtgaag ctagtagcat gaaaattgaa tgtgcgttat ttttcttttt cacttactgg 660
tacaattttt gcatggttta tgttgttgcc ctgcttgttg tattggttca tgggctggtc 720
tgtgaaataa tttggcgata gccattttct tttttgagat acattgcttt tgctatatat 780
atctagatat ggtgcatatt taaatgcata ataaaaatgt aaaaatctaa aacgtcttat 840
aatttaggac agatgaaagt actagatatt agacattttt agtgttttta ttaaaatgga 900
atatgtaccg cctttgatgc tacaactttt acttagcttt taaaacacac cattctaaat 960
tgtaaaaaaa tattaaaaat gtgttttgca agatgaatat actaaccttt gttatgataa 1020
tagttttcat atgttaatgg aacaagctaa aaagtttggc aaagtatagt cctatagctt 1080
ccatttcgac tcagagagag tatgttgtat ccactaaccg tgtacacaag atagcccaac 1140
taattaatta ttttgtgagc tatcacccaa ccttctgttt atcatggatt catggaaaaa 1200
tgtaattgcc atcattacac taaaaactaa aacttatgaa ggagaaccat tgtcttgcta 1260
tatatgagat gacaaaattt tccaaagaag agagaagccg gcagaaccca tcctgtttca 1320
aatctcttct actacttaag tttctaacgt aggcgtccac aaaacggatt ggtgcacggt 1380
tctgccgatg tctcccacac acgcgcatgg aaggaggcag gcacccttcc ccgccgcccc 1440
ggatctcgcg ccagccccag ccctaccccg cctgcccttc cattcttccc cagccgcccc 1500
ccggtcaacg tcacgaaccc gggcctcgtg ccgttcgccg tggccacgcg gttcgacgag 1560
cgggtcacgg agctgctgag cgcgctcgct gacgcggcgg cggggcgacc aggcaggtgg 1620
gccatcggcg aagcgccatg gtcgtcgtcg gggggcagga accaggcggt gtacgcgcgc 1680
cgcgcgcccg gctcttcatc gcctccaccc gctccagcgt ctccaccacc tccttcatcg 1740
agggccgact gcgaggctcg ccggccaggc agccgagcgt cagttgcgcc gcttggaacg 1800
cctgcttttg ttgatcgttt gttttggtct gatttcggtg ggtctatccg cagagaggaa 1860
gaagcagaag ctctccgaga tccaatccgg cgttgaggaa gctgaatcgc tggtaaatag 1920
atgccgcgac acgttctggt ttggggatcc ccttggctaa caggacatac gacatttggg 1980
gaatgggtag aaaagcagag attagggatt tttcgtttcc gtcggtgcag ttttggtgtt 2040
ccaacggagt tgcgagatgt ttatgtgcct tagtcttcaa tttgggggtt gggggaaaag 2100
taattttatg tttttgtttt gtgtctgcag attcagaaaa tggacctgga ggcaaggagc 2160
ctacagccta gcattaaggc tagtttgctt gcaaagctga gggagtataa atctgacctc 2220
aacaacgtca agagtgagct caagaggata tctgcgccca atgccaggca ggctacccgg 2280
gaggagctcc tggagtctgg aatggctgat actctcgcag tgagctaatg ataggacttg 2340
actgtgtcta cgagactgct cctaacaata aactgaagaa agcaaaagaa atcattcaac 2400
gtattcgccg aagagaactc tacaaggtag tatgatgctt taattgctca tatacaagtg 2460
tcattttgtc atgtcattac acatggttag gatacatagg agattctgtt ttttaacaca 2520
tagttgtccc atgtccatga attcatttga attaatttac tcttcgcaat cttatacatt 2580
aaaatcgtgt tacctattac atcacaactt catgagagca tgcttgttct gtgtagatat 2640
ggtagtcctg ggaggaagag ccatcgccgc tatgtatggc agaaccaccc gcgaaagcaa 2700
cctctctggt ctcgcatagc cagagcagga gcagctcgct tgcgcggccg cagcgctggc 2760
ggtcggcccc gcgtacgagc gcctgcaggt aggccagctt ctgctgcaat gcccgaatct 2820
cggcgtccac gcgcagcagc gtcgtcgcct cctcctccgt cagctcaccc agcttggcca 2880
gcacccccgt cacccccgcg tccgccatgg ctgtcgccgg gaccgaaagg ctaaaactgt 2940
cacaatgacg taaagtttgg ttggtgttgg cggctcacgc aaaaccagac ctttccaagt 3000
tttactttag cggagttttt ttggaacgag agcaaagcag cacagtttca agaatgtggg 3060
gcaatttgaa tgttcgttcc tgctgcactg ctactgcttt tagaattgta gtatgcttca 3120
tcatttattt atttctaaaa aaacttgcat gaattctatc gtgactttta ttgagaaaat 3180
aatgtattca cgtatcttca tgtttctgat aaaggtattt gtatatgcat cggtgctaca 3240
tatgcgaata caagttttgt ttcaactctg aagtctcaag ttgaattcta aactccagtt 3300
tgttttctac tgtgctgctg caggaagcca ggaacccatc cgaacaaggt tgcaatcatg 3360
ataagcagat agagcaagca tatgatgata ttttgaattc gtcgaagcat actttggcca 3420
gcatgatgga gctgcaggag gttcagttat ttgcacacat tgtttttctt tcactcctat 3480
gattttcctc aatatgatca aaatgtttct tttgcaaata atgattgaaa tgtttctcat 3540
tgtactcaac ctcttaaact acctataggc tttgcttgag agtaatcagg ctacaaagga 3600
tgccaatggt attgctgctc tctatattgt tcttgttcta atgtaa 3646
<210> 7
<211> 3646
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 7
atggcacact ttgatgaact agaggataaa acaacagatt atgttgattt atcggttcaa 60
gaatttgctc ttaagcaacc tcaatgtggc atggcttata attactatgg aaatttaagg 120
ctttatgtag tagccaataa agctgaattg gcctcttcaa tatttgaaat cgataaggta 180
aacaaaggcg gagttaatgc atctatgcca gtgaccactt ccactcctaa ttcgaatcaa 240
aattcatgaa ccggttatgg aacaaataga gaatcaagtt tcggtgaggt ataatacgat 300
tcccctaacc catggaattt acctagtaaa aatcctgtag ttaatagtgt actagtaact 360
tctgtcaccg acttgaataa agctttgaat gagtataaaa atgagatgtc taaatttatt 420
gagaatagct tggtgtatag attaagccta gtagaaacac ttataacaag ttgtatgctt 480
caattttttt tgattttttt ggaagctact catagttgga gggtaccaaa tttacaaaaa 540
aaaattggtg attataatag taaatctacc atagaacatg ttagcttgtt tcttgctctg 600
agaggtgaag ctagtagcat gaaaattgaa tgtgcgttat ttttcttttt cacttactgg 660
tacaattttt gcatggttta tgttgttgcc ctgcttgttg tattggttca tgggctggtc 720
tgtgaaataa tttggcgata gccattttct tttttgagat acattgcttt tgctatatat 780
atctagatat ggtgcatatt taaatgcata ataaaaatgt aaaaatctaa aacgtcttat 840
aatttaggac agatgaaagt actagatatt agacattttt agtgttttta ttaaaatgga 900
atatgtaccg cctttgatgc tacaactttt acttagcttt taaaacacac cattctaaat 960
tgtaaaaaaa tattaaaaat gtgttttgca agatgaatat actaaccttt gttatgataa 1020
tagttttcat atgttaatgg aacaagctaa aaagtttggc aaagtatagt cctatagctt 1080
ccatttcgac tcagagagag tatgttgtat ccactaaccg tgtacacaag atagcccaac 1140
taattaatta ttttgtgagc tatcacccaa ccttctgttt atcatggatt catggaaaaa 1200
tgtaattgcc atcattacac taaaaactaa aacttatgaa ggagaaccat tgtcttgcta 1260
tatatgagat gacaaaattt tccaaagaag agagaagccg gcagaaccca tcctgtttca 1320
aatctcttct actacttaag tttctaacgt aggcgtccac aaaacggatt ggtgcacggt 1380
tctgccgatg tctcccacac acgcgcatgg aaggaggcag gcacccttcc ccgccgcccc 1440
ggatctcgcg ccagccccag ccctaccccg cctgcccttc cattcttccc cagccgcccc 1500
ccggtcaacg tcacgaaccc gggcctcgtg ccgttcgccg tggccacgcg gttcgacgag 1560
cgggtcacgg agctgctgag cgcgctcgct gacgcggcgg cggggcgacc aggcaggtgg 1620
gccatcggcg aagcgccatg gtcgtcgtcg gggggcagga accaggcggt gtacgcgcgc 1680
cgcgcgcccg gctcttcatc gcctccaccc gctccagcgt ctccaccacc tccttcatcg 1740
agggccgact gcgaggctcg ccggccaggc agccgagcgt cagttgcgcc gcttggaacg 1800
cctgcttttg ttgatcgttt gttttggtct gatttcggtg ggtctatccg cagagaggaa 1860
gaagcagaag ctctccgaga tccaatccgg cgttgaggaa gctgaatcgc tggtaaatag 1920
atgccgcgac acgttctggt ttggggatcc ccttggctaa caggacatac gacatttggg 1980
gaatgggtag aaaagcagag attagggatt tttcgtttcc gtcggtgcag ttttggtgtt 2040
ccaacggagt tgcgagatgt ttatgtgcct tagtcttcaa tttgggggtt gggggaaaag 2100
taattttatg tttttgtttt gtgtctgcag attcagaaaa tggacctgga ggcaaggagc 2160
ctacagccta gcattaaggc tagtttgctt gcaaagctga gggagtataa atctgacctc 2220
aacaacgtca agagtgagct caagaggata tctgcgccca atgccaggca ggctacccgg 2280
gaggagctcc tggagtctgg aatggctgat actctcgcag tgagctaatg ataggacttg 2340
actgtgtcta cgagactgct cctaacaata aactgaagaa agcaaaagaa atcattcaac 2400
gtattcgccg aagagaactc tacaaggtag tatgatgctt taattgctca tatacaagtg 2460
tcattttgtc atgtcattac acatggttag gatacatagg agattctgtt ttttaacaca 2520
tagttgtccc atgtccatga attcatttga attaatttac tcttcgcaat cttatacatt 2580
aaaatcgtgt tacctattac atcacaactt catgagagca tgcttgttct gtgtagatat 2640
ggtagtcctg ggaggaagag ccatcgccgc tatgtatggc agaaccaccc gcgaaagcaa 2700
cctctctggt ctcgcatagc cagagcagga gcagctcgct tgcgcggccg cagcgctggc 2760
ggtcggcccc gcgtacgagc gcctgcaggt aggccagctt ctgctgcaat gcccgaatct 2820
cggcgtccac gcgcagcagc gtcgtcgcct cctcctccgt cagctcaccc agcttggcca 2880
gcacccccgt cacccccgcg tccgccatgg ctgtcgccgg gaccgaaagg ctaaaactgt 2940
cacaatgacg taaagtttgg ttggtgttgg cggctcacgc aaaaccagac ctttccaagt 3000
tttactttag cggagttttt ttggaacgag agcaaagcag cacagtttca agaatgtggg 3060
gcaatttgaa tgttcgttcc tgctgcactg ctactgcttt tagaattgta gtatgcttca 3120
tcatttattt atttctaaaa aaacttgcat gaattctatc gtgactttta ttgagaaaat 3180
aatgtattca cgtatcttca tgtttctgat aaaggtattt gtatatgcat cggtgctaca 3240
tatgcgaata caagttttgt ttcaactctg aagtctcaag ttgaattcta aactccagtt 3300
tgttttctac tgtgctgctg caggaagcca ggaacccatc cgaacaaggt tgcaatcatg 3360
ataagcagat agagcaagca tatgatgata ttttgaattc gtcgaagcat actttggcca 3420
gcatgatgga gctgcaggag gttcagttat ttgcacacat tgtttttctt tcactcctat 3480
gattttcctc aatatgatca aaatgtttct tttgcaaata atgattgaaa tgtttctcat 3540
tgtactcaac ctcttaaact acctataggc tttgcttgag agtaatcagg ctacaaagga 3600
tgccaatggt attgctgctc tctatattgt tcttgttcta atgtaa 3646
<210> 8
<211> 10605
<212> DNA
<213> Zea mays
<400> 8
aggaatctta aacatgtgga acaggtgctc aacacattta gcaactagtt gttgatgacc 60
cataactttg cagccttcat aatgcacaca attgatgcat caattgcata cctcctgtct 120
ttgtcaacat tttcaacacc ttttttcttc tcatcaacag gaggcgatgg aatccaaaaa 180
gagtgacaac aaaaatatta gtataataac taaactctaa gtctaccaaa cagtgaaaga 240
atagtgaaac aggaaatacc ttaattctta tcattatgtt aataaattta aaattgaact 300
aaaaaacatc attaggtgag atggatctat ttgtcggttc cctgttaagg atcttgtatt 360
tcacacaaga aagtgaatgg agcaaacaca cactacatca tctgacatgt ttagttgtgt 420
tgcaatttca aaatatctaa gcctgtcgga tccattgaac aatagtagta atgtggcatg 480
tcaaaaaatg tcaaacatgt taatatagca ccattttttg tgatgcagaa atgacccgag 540
attacctgat atgtcataac aacgagctca atagggttag catcaaactt cgcacttacg 600
ttgcaggttc ccatccatgt cagcttcatg tgcttcgttc ttgtttgatg gaactccttg 660
aaaacatcta cacatttcag tgcctcatga gactcgccat agcgagcggg gtggaggatg 720
gtgacaattt cggcagcctc gccgggggca gcactgcgac gagcgaggcg ggaggggtgg 780
caatctcgtg ggggaggggc caggcacgac cgtccaacca cggcgggcgg agacacggta 840
tccatgtgag atatagccac ctcatctagc cttatctcca agattttaaa tcactgatca 900
tcataagcga tcagatggag gccagttcac atcgaccgac aggactgata ctaacaggac 960
catccacact tgcacatact ataaagatta ataagagatt acataagaac taagtagtga 1020
atcagacacc cattgctgac cttgttaatc agcccatcgc caatgaaggt gctgctgatc 1080
ttcttgaaca acctgtacaa tgcctcgacc ttgttcacgc tgactgcaag aatgacaagc 1140
agaggaaacc caaccaggca ggaaaatgat gaccaacact gaatagaaaa agtaaatgaa 1200
cacctccagc tatcaaaatt ttaaagcaat gtgaagtcct caaagaacca ggacaacact 1260
catgattttt tataactaag ggaattgttt atcatcaatt cattctaaaa tacaagacaa 1320
tcaaaagaac taagcaaagc atgagataca aaaattcaaa gcacatgtat agtgtcttgg 1380
taaaaaattt acaagatggt gaatgaattc aactcaggtt gtctacttca gcattagttt 1440
gcactgtcca gaaaaagaac aacagcaaga ttggaataat gctatggcca ccagaataaa 1500
aggtcagagc tgtcttttaa tgctaatatt gttcatgcca aacatttctt tgttagcttg 1560
tgaatttata cttggacact ggactgggcc ttgatcgacg ctggcaatat catgctgaac 1620
tctgaaggca ccaaaactgt tagctccttc cctcgtcaat ttgtcaattc aacatgtctg 1680
cttcaaaatg gttatgcgta ggttgaagaa aagttgggag tttacaaaat aatacaatgg 1740
gatgcctgtt ctatcatcta acttaagcca tgtatcaagg ttgcaagtta cataaaatac 1800
gcttatattc tgatggttgg aaccacacat tctacacgtt tcccaaaaca atgaaaaagg 1860
tagttgtcga aagatttaag catctaaagt gtccactctc tctgagagca tcaaaataaa 1920
gtagtacgtc ttatgtttta aactatttat tgaagtacca aactatacgg ctactaaaga 1980
tttatttaga tgagtaaacg aaataattta tggtatataa attaagaagg ggtgattagt 2040
catgaaaaat aaaatgtcac aattaccagc agcacgtgat tttctaaata atttaagcat 2100
gtgcggtgct cttccagata aaacttaggg gacgaccacc tagttcattg aaagagggga 2160
ataaaccaag ctccaacttt caagcttgtc aaggcttgtc attattaatt taaacaggac 2220
agccaattct cagacatgat gttccaaact gctaatgaat atataatgct caaaataaac 2280
aactaggttc ttaactgtca attacaccca caagatgcac ataattagaa aaggtaaaag 2340
agaaggcaaa tggaatacca ggaattatat gactactaaa tcatttattt agataagtag 2400
atgaaataat ttatggtaca taatataaga acgggtgatt agttatgaga aataaaaggt 2460
caccattacc agcagcatgt gttgttctaa ataatttaag cctgtgtggt attctttgag 2520
ataaaacata ggagacgacc acctaattca ttggaagagg ggaacagacg aagctccaac 2580
cttcgagctt gtcaaggctt ggcattatta atttaaacag gacagacaat gctcaatctg 2640
aactgccatt gtatctacaa tactcaaaat aaacaactag attctgaaca accagattat 2700
ttgtactcat tccatgtctc ataaacaagg aaaaaataac aaccagatta tttgtactca 2760
ttccatgtct cataaacttt gggcaccatc catccaacac atccaatcta aacacaccaa 2820
acgatgggga atggaaagag cagtattcga ttcaacaatg gcaaacaaat atcactgaat 2880
tagaccaaga ataaacctaa ttagacaacg acctcccaac catcattcgt caggctgtaa 2940
agaagataaa gctgccatgg ggcatggatc aagcagaaca ccagagatga atccaaacac 3000
acagaaaatc acgcgcgctg tctacaatga caacaagccc cacatttcat tgcagtacac 3060
tgggctacaa aggcacgtac aacaaagagc tagggaaaca ttgcggaggg cacgagagag 3120
cagctaactt gacaatatag cagactgagc ttgcactgtt agcaggcgag gaagggaatc 3180
atggggacgg agaatggggt ccatgcccgc gaaggagaag gcggacgccg ccacggtggc 3240
accggcgcac gcgcacacag ggaacccgca caggcagcca tggatgctgc ctcgccattg 3300
cgccggtcgt ctctgccacg ctcctctctc tctcccgctg catcgccgtg gatggggcaa 3360
gcagagagca gggactgcga cgatctgggc ggaggactcg ccttggagag cgcggacgca 3420
gacgggattc tagggagaga gcgaagacgg ggcgcgcgcg gcgctcgcgc ggcgtggtgg 3480
cggcgagatt agcgggggtg gggggagggc ggagccgtgg tgagggtgtg gacgccctcc 3540
ttaccctctt aagtagtagt agagatataa tccgttccaa aatatccatc cgttcaattt 3600
atatttcgtt tgatcttttt accctaaatt tgattgactc atcttattaa aaaagttcat 3660
aactattatt aatctttatt gagatatcat ttagcatata atatacttta agtgtggttt 3720
tagatttttt ttaaaaaaaa aaattcgcaa aaattaaatg aaacgaccca atcaaacttg 3780
aaaagtaaaa ctaattataa atttgaacgg aaggagtaag aggatgtttg aatgtactag 3840
agctaatagt tggttgcttt aaaatttgct agtagaatta gctagctaat aaatatctag 3900
ataactatta gctaatttgc taaaacagct aatagttgaa ctattagcta gattgtttgg 3960
atgtattcgg ctaattttaa tggctaacta ttagctatag tacaatattc aaacacctcc 4020
taattaaaat ggacaaatat ctcttctttt ggtcccttgc gttagatttt tcatatctcc 4080
ttatttagta taaaagaatc atcaaaaagt ggacaacccc tagtggaaca ccattttagt 4140
agtggttgca tgaaaccttt cgcgcaccag tttctatgtg tcactctaaa aatgggacag 4200
catgtacgta gtgcctatat atatacaagt catctatcgt tgcctcctca gttcatcact 4260
aatcacactt attgtgccct cgacgagtat ctatagctag ctcattaatc gattcggggg 4320
tgtgttgtcg aaggcggcaa tggcgagcta ctcgtcgcgg cgtccatgca atacctgtag 4380
cacgaaggcg atggccggga gcgtggtcgg cgagcccgtc gtgctggggc agagggtgac 4440
ggtgctgacg gtggacggcg gcggcgtccg gggtctcatc ccgggaacca tcctcgcctt 4500
cctggaggcc aggctgcagg agctggacgg accggaggcg aggctggcgg actacttcga 4560
ctacatcgcc ggaaccagca ccggcggtct catcaccgcc atgctcaccg cgcccggcaa 4620
ggacaagcgg cctctctacg ctgccaagga catcaaccac ttttacatgc agaactgccc 4680
gcgcatcttt cctcagaagt gagtccgatg ctgccgccat tgttcttgca tccatccagc 4740
atcgtacgta cgtcctctat acatctgcgg atcatcatgt gcgcatgttt gtggcatgca 4800
tgcatgcatg tgagcaggag caggcttgcg gccgccatgt ccgcgctgag gaagccaaag 4860
tacaacggca agtgcatgcg cagcctgatt aggagcatcc tcggcgagac gagggtaagc 4920
gagacgctga ccaacgtcat catccctgcc ttcgacatca ggctgctgca gcctatcatc 4980
ttctctacct acgacgtacg tacgtcgtca cgaatgattc atctgtacgt cgtcgcatgc 5040
gaatggctgc ctacgtacgc cgtgcgctaa catactcagc tctttcctat ctgctgcgcc 5100
aatttgcagg ccaagagcac gcctctgaag aacgctctgc tctcggacgt gtgcattggc 5160
acgtccgccg cgccgaccta cctcccggcg cactacttcc agactgaaga cgccaacggc 5220
aaggagcgcg aatacaacct catcgacggc ggtgtggcgg ccaacaaccc ggtaactgac 5280
tagctaactg gaaaacggac gcacagactc catgtccatg gcggcccaca aggtcgatgc 5340
taattgttgc ttatgtatgt cgcccgattg cacatgcgta gacgatggtt gcgatgacgc 5400
agatcaccaa aaagatgctt gccagcaagg acaaggccga ggagctgtac ccagtgaagc 5460
cgtcgaactg ccgcaggttc ctggtgctgt ccatcgggac ggggtcgacg tccgagcagg 5520
gcctctacac ggcgcggcag tgctcccggt ggggtatctg ccggtggctc cgcaacaacg 5580
gcatggcccc catcatcgac atcttcatgg cggccagctc ggacctggtg gacatccacg 5640
tcgccgcgat gttccagtcg ctccacagcg acggcgacta cctgcgcatc caggacaact 5700
cgctccgtgg cgccgcggcc accgtggacg cggcgacgcc ggagaacatg cggacgctcg 5760
tcgggatcgg ggagcggatg ctggcacaga gggtgtccag ggtcaacgtg gagacaggga 5820
ggtacgaacc ggtgactggc gaaggaagca atgccgatgc cctcggtggg ctcgctaggc 5880
agctctccga ggagaggaga acaaggctcg cgcgccgcgt ctctgccatc aacccaagag 5940
gctctagatg tgcgtcgtac gatatctaag acaagtggct ttactgtcag tcacatgctt 6000
gtaaataagt agactttatt ttaataaaac ataaaaatat atatatgttc ttgaatataa 6060
aattgataac caaattaaaa ttcgaaccat cacttataca taattttact ttatttttta 6120
taaaacgtga acgggaagga ctaccgtgaa tgactataga accaatcata ctagtataaa 6180
atatatgatg acactacggg agagacaaac tttgtctggc gctaaatatt ttgccgagtg 6240
tgaattcacg ggcactaggc aaagatcttc tttgccgagt gttacgctgg gcaaagtaag 6300
acactaggta aatcagtcat ttgccgagtg tccgccacta ggcaaagcaa aacactggca 6360
aatcaaaagt ttacctagtg ccagacacta ggcaaaaaaa aaacgctcgg caaatcggaa 6420
gtttccctag tgccagacac tagacaaaga aaaacacttg ataaactagc gtcgtcagct 6480
aacaccatcc accaaccgtt aacgttgccg agtatctgac ttcgacactc ggcaaagaag 6540
gtctctttgc ctagtgtcgg tctggaacac taggcaaaga ggcactttac ctagtgtcgt 6600
attttgacac tcagtaaaat aatttttttt ctttctgctt ccaaactttt tatgatgtgt 6660
tcctatagca cctagaacta catgtcaagt tttggtaaaa tttttgaagt ttttgctata 6720
tttacttaat ttattttatt taattgaatt tcttttgata attcaaattt gaactcggca 6780
aggtaagaag cgagggtagc ctggaaacac actttgccta gtgttacact cggtacagga 6840
gcctcccctg cctagtgctg cactcgacaa aagattcgcc tttgcctagc gctgcactcg 6900
gcacaggagt cgcctttgcc tagtgctgca ctaggcaaag cctccgttac cgtgccttcc 6960
atcgtcatgg aaacttttct tcgccgagtg acgtgtggca ctaggcaaag tttttgccga 7020
gtgcccgaga aatggcactc ggcaaggact ctttgccgat cccttcgttg ccgacttctt 7080
tttgccgagt gcaacactag gcaaaccatt tgccgagtgt aaaagaggct ttgcctagtg 7140
tctgtggcac taggcaaaga agacgagtcc tgtagtgaac ctagtaggcc agtgcgggac 7200
cattccaaaa aatacctata aaaataaatt taatattaaa ttaaacatat ggtccacgta 7260
ccaagatatt aaactcaaaa gaacaattat tacaatttat cttagctaaa aggccgagaa 7320
aaagtatatg ttaaaaagga gtgtgatccc atttttatag ctcgctcggt cgatcgcccg 7380
tccactttta ggtaacgagg tggtaccatg taggagtgtt gcgttgcgtg cgacttccta 7440
tcatgttggg cttaggtggc ttctcacgac ccaatgatag gcgagaagtg tggaagatga 7500
acaaacctac ttgtttcgtg cacgacgcat gtgtttgaac aacgagttag attagaaaaa 7560
aaatataatg actttttttt ttgcaaaagt gaggataatg aaaaccagaa aaactggtgc 7620
ttcataagag tagagatttg atggtaaata tagtagtaat gcaatggcta tactacacgc 7680
gagagtccaa tggcaagccg gtgtgttggg gcgaaggcga agacgctacc cttcgctcca 7740
ggcctttgtc aactcgctgc accaacagag gcaagatgac cggcgcggcc cacccttcgt 7800
cctcttcact gcaagacgaa ggcctacgac gaagtctctc catcccacgt cctcgcctta 7860
cctggaggcc cacgtgggat tcggcccatc gtaacaggcc ccgcacggac aggcgtgtta 7920
cgggtttgat ttgtaatagc ttttctgtaa tgacagtttg taaccctccc ttatgggaat 7980
attctgggga taatccaggt gtctgagggc ataagcgtcc ttacatcggg acgttgggcg 8040
ctcgggcacc tataaatacc cccgtacagt gcccttgaga ggctggatta acatagcaat 8100
tgccatctcg agttaaacct tgcttgcatc ctttccactc tcccgttgga tcaacttgcc 8160
caagagagct agttccaaca tttggcgccc accgttcgtg ctacgagcaa accacccgcg 8220
atggcaccca aaagagctag ttcgaaggca gccccatccg tcgacgaagc ggcgaaggca 8280
gcactgctag ctgagaaaaa gggcaaggcc ctcgcagaca acacccacca agaagctggc 8340
gaagacgaag cactcagtaa gagacagcgc aacgatcaac acactctcga aggcaccctc 8400
cgcacctaca gctccggagg ccaaccacaa gtaccacccc taggcttcgc tccactagag 8460
ggcgaggaca caacagagga cggcgaagtc atcggcgtct cagcagaaga acaactacag 8520
ttatgggccc tgcgcctcaa gaaccgcaac ctccaaaagc agaaagaaat cctcgaagcc 8580
aagcgccaac gcgtctccgc gcaagccaaa gtgcgttaga tgatacgaga cgaggagcag 8640
agggcccggg aactagagca agagattgcg ctcatgcaga gcgaaggaca gcatgatcta 8700
cagcatggcc cacccctcca gcagcgcgcg ccagctagag atttattcat tccccagcgc 8760
gggcccttca tcccacacgc cgcagctttc caaggcatca actaccttga tgagcgaagc 8820
cccctggcgc cgcaactcca agtgtcacct tggcccgcca acttcagggc agggagctac 8880
cccaagtaca atggcagcac cgacccagca caatacatca tgagctatca agtcgctgtc 8940
gcatcatccg gaggggacga cgccacaatg gccaagtcct tcatcatcgc cctcgaaggt 9000
ccggccttga cctggtacac caggttgccc ccactgtcca tcgactcctg gcgaagtctc 9060
cgggacaagt ttctgcttaa ctttcaaggg taccgcccag acatcgatgc cttggccaag 9120
ctgtcactct acaaacaaca agagaaagaa accctacggg agtactaccg caagttcctg 9180
gctctcaagt cgcaactgcc ctcggtcgac gaccaaatcg ccatacacta cgccatcagt 9240
ggccttcggg ctggcgtcct atacagtcac tgcatcaggt acccacccaa aaacctccaa 9300
gagctctatc agttgtttga aaagtacgcc agatccgaag agctccatca gcgcaaggtc 9360
gagtctcaaa gaaagcccaa ggaccctccg cagtctagcc aaacatggac aagaccttca 9420
cagtcagact ccggtcggga caaccgcagt cagcagcagg tgcataacat tgccaaccag 9480
caccccgcca gcgaagcccc tcgccgccaa gattatcccc ccagggccgc ggcaatggca 9540
cgcgtggtcg gggctgggga cgggcgcaac agccgtgcag atattactgc ctgttttcac 9600
ggcgaagact gcacgcaccc aaccaaggat tgtccggaaa cgaaggccac cagggacagg 9660
atgtctcggg cacaacccgc cgacaaccca agagttgtcg cgcacacata ccaacaccac 9720
cacccacaac catacaacca cggccccgcc cagcatctac ccaaccacgc atatcaacac 9780
caccaggagt tacaagtcat accacctcca cccccgcctc cgcatcaacc aaacatccac 9840
caccaaaatc accccaagca ccaaaacagg aagacttcgc tgatcagccg tatcgcggag 9900
tcattcacat gatcaccgga ggggtccagc attgactttg acacgaagcg acaaaagagg 9960
aatcactacc gaagcatcaa ccacgtcgcc atcaccggct cggtcgtgca aacgaagtgg 10020
tcacatgtgc cgctaacctt cgacgccaga gatgttgatc tgcgcagcgc accccacatt 10080
gatgccatgg taatcaactg cagtgtggca ggctgggacc tgcacaaagt cctagttgac 10140
aacggcagcc aggcggacat catcttcctc catgccttcg accgcatggg catcagccac 10200
agccctctca agccttcgaa caatccccta tatggcttcg gcggcaaggg caccttccct 10260
gtgggcaaga tagagctacc cctatccttc ggcgtagcac ccaatgcgcg aagcgaatag 10320
gtcacctttg acatcattga catggtctat ccctacaacg ccataatggg tcggggctct 10380
atcaacaaat ttgaagcggc aatccacgga ttatacctct gcatgaaaat tccgggtcca 10440
caatgcgtaa taacggtgta cgggaaccag cagactgcgc ataacattga gagagatttc 10500
gttcccgatc aacggaacgt acactgcctt acgacgcagc gcgaagtccc cgaggctacc 10560
tgcctagctg ccaacaaaaa tgaaaaggca cagctaaaaa gcaac 10605
<210> 9
<211> 11001
<212> DNA
<213> Zea mays
<400> 9
aaatggccga agctattttg gatgaagcca tctctcgact attaaacgaa gctgcggaag 60
cagttttaaa agaagaatag ttgttattgt aaaaacattt ggaatgtaat atttgctgaa 120
caaagtgtgt aatattttta taatttgaat gtaatatata agctgctcgt aactcaattc 180
tttacgatgc atgaaacttt acgtacatac cgtttttgag ccttcggcga aaaaacacct 240
tcccttcttt tcatgcttcg tgaagaatat ccatacttcg taaaaacatt atgcttcata 300
agcaatagat ctctttttca tattagagtt gatgaagttg tacttgttca aaacttattg 360
tgccttggca ctgcttcttc gaaacaatct cgaagatcaa cattgtatcc ccttcttgtg 420
ttattgatgc aatatgatgt tatgctatgc aaaatgatgt gatgatgtta tgctatgcaa 480
aatgatattt atgtcgaaga tacataaaca ttcccacagt agagcacaca atctttttgc 540
cgtttatttt tcggcttcac cgcttatttt tcggtgtatc agcgctgact tttcgctgta 600
agcctccctt aggtgcttct tcgcctttta cttcggcggt atttgcgttg actttttgcg 660
cttcgcctta tacttcggtg gaatcagcgt ttatttctcg ctgtaagctc tgcattccct 720
ttggaacgac ttttgagcag aaaacttacg ctgcgctccc ttagaaatga ctttttgtaa 780
cttcggcaaa cttacgctgc gtttcataga acgacttttt tgtagtttcg gagatacttt 840
ctgtagccac aagttcttaa gaacgagttt tcatgcttca tcaacttttt gaattccgta 900
agtctgtgga gaagatatat tttcactatg acaaaaacaa agctgttaca agaaattgaa 960
aacaacaaga aaaacttagg ctttcaatga ttgttcttta ttaaaaagaa aaatgataac 1020
taatgcaaga actatttcag aagtaggata tctgttagta gatgtgcttt gactctggca 1080
caatactgtt gactgtgcga gcttcggact cctctctgaa gtctcgttgc tgatgagtgt 1140
gctggctccc ttctggctgc tggcctcgtt gtattggtgg tggaggtgga agctgttgcc 1200
aagatgcctg aggttggctt gccgaagcaa cagaagctgc aggatggtta cccttaccca 1260
catactctgg aatgtacggt gagtggtacg aagcagtatg cataacttgc ttcggctggc 1320
tctgttgggc tgcagtttct gctatctctt tctgtttctg gatggtgaca tggcacatcc 1380
tggtagtatg gcccttgtcc tcaccgcaga atagacaata aattttcctg ggctgatccc 1440
caaaccttcc tccgaagccc ctggctcctc tgccccttgg ggctggaggc cgaggataac 1500
tctgttgctg ccccgaagcc taggaggaat attgcggcct ctgttgctga cttcccctgt 1560
cgtcattctg agtggaatga attgatctga catgtctagg gtgtactctc cctccgaagc 1620
ccctggtcat ctcggagaat ctgtaagaag gcccccaaca cctctctttc ccattcgttt 1680
aggtgagaaa acacattggg gagcactagg gagttctttc ctagtgaggc gtctgtgatt 1740
tgataatcac aagattaagg atttcattag tgcatgtgta gtagcaagtg tgcatccacc 1800
ttcctcatta agcttgttta ggataagcca gagtttgtgc cggttactct tgatgttcaa 1860
caacaccaag atggcttggt ggtaattaag agcttggtga tctctcagtg gtgctcgtga 1920
gagtcccaac tcattgtgta ataaaagatt ataggtgatt caccatgccg gagtggtgaa 1980
taatcaaccc gtagagagca ttgagtcctt gaatggatcg atggggggct acacccttgt 2040
gtgggtcaag tcagagtttt agcagttctt gcacccatga tctcatcgtg aagcatagat 2100
aaatttaaat tcttttgaat tatttatata tgacaacact attcgtcgct ctaggtgact 2160
atcacctacc ctaaaatgac ttaacaaatc tttattaatt gttaagtcat tcacattttt 2220
gttaatccac tccaaagtca gggtgtttag tgtttttaca tccatgtctc cttagactca 2280
cggtgtctct cccagattct ctctcaccct cacctctctc tcactagcca ctagggaacg 2340
caacacccat cgatggctct tcgccccatg aaacgttcac acaatcgcaa ttgtcgaggc 2400
atgcatggct gggagagcag acatggaggc atacgtgcta gggttgcaca tgggcaagag 2460
ggtgggtgtg gctattcaga tatgcatggt gagcaagatg ggtgaggttg tgggcatgat 2520
gaggggataa ggaagaataa gatctctttt gttaggctgt ctccagcagc tatcgtatcc 2580
cattccctat cgcatcccct attttaaact ttactatgca aacaatgtaa tatatagtgc 2640
agattcccta ttttacacaa tgtgttgtag acaaccttgg agctcttgca taaaagctct 2700
agttttggct ctagctcctc tgagaaaaca atccccacca tgtttttagg aagaatccct 2760
gaagggcacc ccatttggtt ggaaatacat ctcctcctac aggattatgt ttgacttttt 2820
tttgcaatgt gggacccaca ggggagagga ggacgagaag gaaccggaga gcctattttt 2880
tgggctcctg gcttcgcttg gtttctaggg gcggctcctt cctattttca caaaggagct 2940
agtagaggag cctcccattt catgattttt tgaaggatct atttaaggag ccttgaaaga 3000
gccctaccaa ggtaggccta gaaataataa aggaggaaaa agagaaggta tcacaacttt 3060
tgtctacaac gtgaaaatgt ttggctaaat agataaaaca gtttgaattt tatcgattca 3120
attgtttatt gagggcatgt ttgggagggc tttagttcta gcttctttcg cgaaaaatcc 3180
agagccctac aaaatgacgt ttggtaaaac gacttcttcc gaaaaacacc caaaaaccca 3240
agatatttta tactacgaag gaaaggtcac acatcctagt tagcttcact ggttctagct 3300
ccttccaatt ttgcaaaaaa gtcacaaagg ataagccatt ttttcaaatg atttgtgaaa 3360
tgcctacgct aaaaagtcta cttttccaaa aaaactagag ctagagccgt ttttggcaag 3420
tcagaaccct accaaatagt ccctcagttt aagcaaagtg aggctatact gaagctaaat 3480
tatgccaaat tgggcctaca tctccatatt ttcaaccaaa tgctttaggg tttcttgtaa 3540
tcgacatgat ttgtttcttc ataaatagta tatggaccgc tccaaaatac tccatccgtt 3600
tcaatttata ttacgtttga tctttttacc ctaaatttga tcgactcgtc ttattaaaaa 3660
agttcataac tattaataat ctttactgtg atatcattta gcatataata tactttaagt 3720
gtagctttga tttttttttt gcaaaaatta aatgaaacga cccaatcaaa cttgataaaa 3780
aagtaaaact aattataaat ttggacataa ggagtaggag ggtgtttgaa tacactagag 3840
ttaatagtta gttgtcttaa aatttgctag tacaattagc tagctaacaa atatttaggt 3900
aactattagc taatttgcta aaaacagcta atagttgaac tattagttga actattagct 3960
agactgtttg gatgtattca actaatttta gcagctaact attagttata gtataatatt 4020
caaacacctc ctaattaaaa tggacaaata tctattccct tggtcccttg cgttagattt 4080
tccatatatc ctcatttagt ataaaaagaa tcatcaaaaa gtggacaacc cctagtggaa 4140
caccatttta gtagtggttg catgaaacct ttcgcgcatc agttactatg tgtcactcta 4200
aaaatggggc agcatgtacg cagtgcctat atttatacaa ggcatctatc gttgcctcct 4260
cagttcatca ctaatcacac ttattgtgcc ctcgacgagt atctagctag ctcattaatc 4320
gatcaatcgg ggtgtgcggt cgaaggcggc aatggcgagc tactcgtcgc ggcgtccatg 4380
caatacctgt agcacgaagg cgatggccgg gagcgtggtc ggcgagcccg tcgtgctggg 4440
gcagagggtg acggtgctga cggtggacgg cggcggcgtc cggggtctca tcccgggaac 4500
catcctcgcc ttcctggagg ccaggctgca ggagctggac ggaccggagg cgaggctggc 4560
ggactacttc gactacatcg ccggaaccag caccggcggt ctcatcaccg ccatgctcac 4620
cgcgcccggc aaggacaagc ggcctctcta cgctgccaag gacatcaact acttttacat 4680
ggagaactgc ccgcgcatct tccctcagaa gtgagtccga tgctgccgcc attgttctcg 4740
catccatcca gcatcgtacg tcctctatac atctgcggat gatcatttgc gcatgtttgt 4800
ggcatgcatg tgagcaggag caggcttgcg gccgccatgt ccgcgctgag gaagccaaag 4860
tacaacggca agtgcatgcg cagcctgatt aggagcatcc tcggcgagac gagggtaagc 4920
gagacgctga ccaacgtcat catccctgcc ttcgacatca ggctgctgca gcctatcatc 4980
ttctctacct acgacgtacg tacgtcgtca cgaatgattc atctgtacgt cgtcgcatgc 5040
gaatggctgc ctacgccgtg cgctaacata ctcagctctt tccgatctgc tgcgccaatt 5100
tgcaggccaa gagcacgcct ctgaagaacg cgctgctctc ggacgtgtgc attggcacgt 5160
ccgccgcgcc gacctacctc ccggcgcact acttccagac tgaagacgcc aacggcaagg 5220
agcgcgaata caacctcatc gacggcggtg tggcggccaa caacccggta actgactagc 5280
taactgcaaa acgaacgcac agactccatg tccatggcgg cccacaaggt cgatgctaat 5340
tgttgcttat gtatgtcgcc cgattgcaca tgcgtagacg atggttgcga tgacgcagat 5400
caccaaaaag atgcttgcca gcaaggacaa ggccgaggag ctgtacccag tgaacccgtc 5460
gaactgccgc aggttcctgg tgctgtccat cgggacgggg tcgacgtccg agcagggcct 5520
ctacacggcg cggcagtgct cccggtgggg catctgccgg tggctccgca acaacggcat 5580
ggcccccatc atcgacatct tcatggcggc cagctcggac ctggtggaca tccacgtcgc 5640
cgcgatgttc cagtcgctcc acagcgacgg cgactaccta cgcatccagg acaactcgct 5700
ccgtggcgcc gcggcaaccg tggacgcggc gacgccggag aacatgcgga cgctcgtcgg 5760
gatcggggag cggatgctgg cacagcgggt gtccagggtc aacgtggaga cagggagcga 5820
ggtacgaacc ggtgaccgga gaaggaagca atgccgatgc cctcggtggg ctcgctaggc 5880
agctctccga ggagaggaga acaaggctcg cgcgccgcgt ctctgccatc aaccccagaa 5940
gctctagatg tgcgccctac gatatctaag acaagtggct ttactgtcaa tcacatgctt 6000
gtaaataagt agactttatt ttaataaaat ataaatatat atatattctg ataaccaaga 6060
ttcgaaccct cacttataca caattttatc ttatttttta taaaatgaga atggaaagga 6120
ctaccgtgaa cgactataga accaatcata ctagtttaaa atgctcgtaa gctatgacga 6180
acctagtagg ccggtgctgg accattccaa aaaacctata aaaataaatt taatattaaa 6240
ttaaacatat ggtctatata tcagatatta aactcaaaag aataattatt ataatttatc 6300
ttagctaaaa ggttgagaaa ggtatgcgtt aaaaaagagt tttaacccat ttttatagct 6360
tatttgatcg cccgtccact tttagggagc gaggtggtac tatgcagaag tgttgcgctg 6420
tgtgcgactt actatcatgt tgggtttagg tggattctca cgacccaatg atagacgaga 6480
agtgtgggag atgaacaaac ctacgcattt cgcgtacgac acatgtgttt gaacaacgag 6540
ttagattgga aaaaatataa tgaccttttt tgcaaaaatg actacaatga aaaccaggaa 6600
aaccggtgct tcataggagt agagatttga cggtaaattg ttacgatcta ctggtatttg 6660
ctgcgaggat gtattcgctt ggtgaaaaca gaattacaga gtagcagtag cagggaagac 6720
agtagcgaga ggagaagaag aaacttgagg aagaagaaga taaatgtagt tgttacatcc 6780
tgccttcgcc gtaggtctca gcgagcatat atcttcaggt cctccattct gggccctgga 6840
atctcacatt ggccttacgc tggcgtgttc ctcttctcgg cccaactgta gtcttctctt 6900
gaggcccacc agtctccaca ttcctttgtt gctgctatag ctcctcggac acggctgctt 6960
ccgcctgctg ctgcacctgg atgtcttctg aagtcgactt gcgtggaggg acagtgctgc 7020
cattcccctc ccgataacac gctgcttgtc cccaagcagg cgctcgaggg aacctctgac 7080
gaagtggaat caggtcctcc caagttgcca gagatggatg caactcagac cacagaatca 7140
accgttgtga tgctccatta ggcccccatc ggcattgtag tactcgttca ggaatttggt 7200
ggttgtcaag gcgatcagga agctgtgcca ccaccactaa agaacccact gccttcttga 7260
gttgtgaaac atggaacacg ggatggatag tagaagtaac tggaagctcc agcctgtaag 7320
caacagatcc caacttagca gcaactggaa atggcccaaa aagcgaaaat ctagcttctg 7380
atttgcccga ggtgcaagcg atgactgcac ataaggctgc ataacagcct tctcttgcag 7440
ccactctccg aggataggca caggagtgga atccaaaatg tcaatgccaa aatgcttggg 7500
tgcataacca tatagcacct caaatggaga cattttcaat gctgaatgcc aactagaatt 7560
gtaccaaaat tctgccaagt acaaccagtc aatccatttg tgaggacaag catgcacaaa 7620
acatctcaaa aaggtctcaa ggcactgatt aactctcttt gtttgtccat cggattgagg 7680
gtgataggaa gaactcatat tcagtgatac accagccagg gtaaacaatg atttccagag 7740
ctgactagta aagattttat cgcgatcaga gaccatagca gatggcatac catgcaggcg 7800
ataaatgtgt tgcataaagg ccttggccac cactgcagct gtgaaggggt gtttaagggg 7860
aatgaaatgt ccaaacttgg agaatttgtc aaccacgaca agaatacaat ttttacctcc 7920
ggacacaagc aagccttcaa caaaatccat agtgatcgtt tgccaagctc ctgaaggcac 7980
atgaagaggt tggagtaggc ctgggtattt cactctctca ggtttagctt gctgacaagt 8040
ggcacatgct gcaatgaact ggatgacaga tttcttcatg ttcggccagg caaacaactg 8100
cttcagccga tggtaagcga ctgctatacc cgagtgaccc ccaacagcag aactatggag 8160
agcagacaat atagactgtt gaagtgtatg attgttacca acccaaatac ggcctttaaa 8220
cttaagcaac ccttcttgaa gagtaaaatg aggaacaaca tcttggtcaa caaccaactt 8280
agataacaag gtcttagccg aagggtccaa caaatatcca tccattacca aacgagtcca 8340
ctatggtgaa cagactgaga gagcatgtaa tgtgatagca tgttgtcttc tcgacaaggc 8400
gtcagcaact ctattttcat gtccatgctt gtatacaatc ttatattgca accccaaaag 8460
tttagtaaag acttttgctg ccatggagtg ttaagccatt gctcattcaa atgcaccaaa 8520
ctcttttggt cagtataaat aacaaactcc ccatgaagta agtaagctcg ccattgttcc 8580
accgcgacca atatggctaa gtactccttc tcataggttg ataagccttg agtcttaaca 8640
ccaagaggtt tgctgagaac gctaatggat gaccattctg caaaagtaca gctcccaccc 8700
cattcttgca agcatcggtc tcaatagcaa aaggttggtg aaagttggat aatgctaaca 8760
ccggggctga gatcacagct tgcttcaagg tattgaagga gatttcttga tcttgagtcc 8820
aaacatagaa cacccctttc tttcacagtg catttagagg tttggcaata atagcaaaat 8880
gactgacaaa tcgcctataa taacccgcca aaccaaggaa gctccttaac tctttaacat 8940
tggagggcac aggccagttc aacacagcat caacctttgc aggatcagta tccactccag 9000
cagcactgat cacatgaccc aagtaagcaa tagatgtttg agcaaattta cacttagact 9060
tcttgacaaa ccagtggtct ttttggagaa tggtgagaac ttgggccaag tgagatacgt 9120
gatcgtcaaa tgacctgctg tagactaaga tgctatcaaa gaagactaca acacacttcc 9180
tcaacaaaag ggccaaagaa gagttcatag cgccctgaaa ggtaccaggt gctcccgaca 9240
atccaaaagg catgactcaa aactcaaatt gaccatgatg tgtctagaac gctgttttaa 9300
actcttccct aggcttcaac ctcacccgat gataccctga agccaagtcc aatgtggtga 9360
accaacatgc accatgcaac tcatccatta actgttcaaa gatggggatg gaaaacgggc 9420
tttgaccgtt aaagcattca aatagcgata atccacacaa aactgaaaag tgccatcctt 9480
ctttctcacc aacagcacag gagaattaaa agatgatgca ctgggtctga taatccccga 9540
ctgaaccatt tctgccacct gacgttcaat ttcatctttc ggagctggtg ggtatcgata 9600
gggtctgata ttaactgggc tggcaccagc aaccaatggt atactatgat cacaacttct 9660
ttctagaggt aaggacatag gtttagcaaa aaccgactga aactggttca acagctgaac 9720
aatttcagga ggaagggtag cctcatcagt cagagcaaca gacacttgag atagggaaat 9780
ctgaaccaac aattcgtcaa ccggctctac agtttcccct tgcaagagca cttgcacacc 9840
atgataaggg atcaacatcc atcgttgttt ccaatgcact tccataggac tgaaagtctc 9900
taaccaatca agtcccaata tcacatcaaa ggattgtaat ggcaacacct tcagatcaaa 9960
ggaaaaccca tacccctaaa ttgtccattg agcttgagta aacacttggg agcaagtcat 10020
aacaccttca ttagccactt taacctggag acaagttggt accaaagtaa tgtcggttaa 10080
cctggaaatc atagtgtgac tgataaaaga gtgtgaactg cttgaatcca caagaataac 10140
gatttcatac tcctgtatag accctctcaa caaaatggat cctttggccc ttgatcttga 10200
aacagcctca gcagatagag ccaagaagag ttgttcctcg tggactgaat cttctgatgg 10260
agaagactca ttcagaaatg caggcataag atcccagact tcctgcaggg catgaagttg 10320
aactgtagta ttgcagacat gtcccctatg ccatttttca gcacaacggt cacaaagacc 10380
acgtgcatga cgataagcac ggagtgcagc cagtttttca tcagtgcttc gatcagtctg 10440
catgagacgc tgcttaggta aggatctcgg cgcactggca cttgctgcag cagtagttgg 10500
ttgaggaaga ggcagggtcg tcttgtgctg ggatttcgac caaataccac gatcaccctt 10560
gcagaattca ctccgcatag gtggagcagc gacctcatcc tgcaaaaaag cgagggaaca 10620
ggcaatatcc agatccatcg acctttgaag cataacaaca gcacgcatat catcgcaaag 10680
accatccaca aaccgtgtaa caaaatataa cggatcaacc ccagactcat acacagagag 10740
ttgatctacg agagaagaaa attgttcaat atattgagtt aaactaccta actatttgat 10800
gcgaaataag tggtgcaaca acagctgata ctggtcctta ccaaaccgtt cgttcatcaa 10860
ttgacacaag agaggccaag acaaacgggg atgacgaaac ataactgaca gaaaccagca 10920
cgctactgta ggcgacaagt gcatagaggc aattttgacc catgaattca gatcaacttg 10980
atatatatca aagtaattct t 11001
<210> 10
<211> 1284
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 10
atggcgagct actcgtcgcg gcgtccatgc aatacctgta gcacgaaggc gatggccggg 60
agcgtggtcg gcgagcccgt cgtgctgggg cagagggtga cggtgctgac ggtggacggc 120
ggcggcgtcc ggggtctcat cccgggaacc atcctcgcct tcctggaggc caggctgcag 180
gagctggacg gaccggaggc gaggctggcg gactacttcg actacatcgc cggaaccagc 240
accggcggtc tcatcaccgc catgctcacc gcgcccggca aggacaagcg gcctctctac 300
gctgccaagg acatcaacca cttttacatg cagaactgcc cgcgcatctt tcctcagaag 360
agcaggcttg cggccgccat gtccgcgctg aggaagccaa agtacaacgg caagtgcatg 420
cgcagcctga ttaggagcat cctcggcgag acgagggtaa gcgagacgct gaccaacgtc 480
atcatccctg ccttcgacat caggctgctg cagcctatca tcttctctac ctacgacgcc 540
aagagcacgc ctctgaagaa cgctctgctc tcggacgtgt gcattggcac gtccgccgcg 600
ccgacctacc tcccggcgca ctacttccag actgaagacg ccaacggcaa ggagcgcgaa 660
tacaacctca tcgacggcgg tgtggcggcc aacaacccga cgatggttgc gatgacgcag 720
atcaccaaaa agatgcttgc cagcaaggac aaggccgagg agctgtaccc agtgaagccg 780
tcgaactgcc gcaggttcct ggtgctgtcc atcgggacgg ggtcgacgtc cgagcagggc 840
ctctacacgg cgcggcagtg ctcccggtgg ggtatctgcc ggtggctccg caacaacggc 900
atggccccca tcatcgacat cttcatggcg gccagctcgg acctggtgga catccacgtc 960
gccgcgatgt tccagtcgct ccacagcgac ggcgactacc tgcgcatcca ggacaactcg 1020
ctccgtggcg ccgcggccac cgtggacgcg gcgacgccgg agaacatgcg gacgctcgtc 1080
gggatcgggg agcggatgct ggcacagagg gtgtccaggg tcaacgtgga gacagggagg 1140
tacgaaccgg tgactggcga aggaagcaat gccgatgccc tcggtgggct cgctaggcag 1200
ctctccgagg agaggagaac aaggctcgcg cgccgcgtct ctgccatcaa cccaagaggc 1260
tctagatgtg cgtcgtacga tatc 1284
<210> 11
<211> 1140
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 11
atggcgagct actcgtcgcg gcgtccatgc aatacctgta gcacgaaggc gatggccggg 60
agcgtggtcg gcgagcccgt cgtgctgggg cagagggtga cggtgctgac ggtggacggc 120
ggcggcgtcc ggggtctcat cccgggaacc atcctcgcct tcctggaggc caggctgcag 180
gagctggacg gaccggaggc gaggctggcg gactacttcg actacatcgc cggaaccagc 240
accggcggtc tcatcaccgc catgctcacc gcgcccggca aggacaagcg gcctctctac 300
gctgccaagg acatcaacta cttttacatg gagaactgcc cgcgcatctt ccctcagaag 360
agcaggcttg cggccgccat gtccgcgctg aggaagccaa agtacaacgg caagtgcatg 420
cgcagcctga ttaggagcat cctcggcgag acgagggtaa gcgagacgct gaccaacgtc 480
atcatccctg ccttcgacat caggctgctg cagcctatca tcttctctac ctacgacgcc 540
aagagcacgc ctctgaagaa cgcgctgctc tcggacgtgt gcattggcac gtccgccgcg 600
ccgacctacc tcccggcgca ctacttccag actgaagacg ccaacggcaa ggagcgcgaa 660
tacaacctca tcgacggcgg tgtggcggcc aacaacccga cgatggttgc gatgacgcag 720
atcaccaaaa agatgcttgc cagcaaggac aaggccgagg agctgtaccc agtgaacccg 780
tcgaactgcc gcaggttcct ggtgctgtcc atcgggacgg ggtcgacgtc cgagcagggc 840
ctctacacgg cgcggcagtg ctcccggtgg ggcatctgcc ggtggctccg caacaacggc 900
atggccccca tcatcgacat cttcatggcg gccagctcgg acctggtgga catccacgtc 960
gccgcgatgt tccagtcgct ccacagcgac ggcgactacc tacgcatcca ggacaactcg 1020
ctccgtggcg ccgcggcaac cgtggacgcg gcgacgccgg agaacatgcg gacgctcgtc 1080
gggatcgggg agcggatgct ggcacagcgg gtgtccaggg tcaacgtgga gacagggagc 1140
<210> 12
<211> 16619
<212> DNA
<213> Zea mays
<400> 12
atcttttatt ggtttgagtt gaacctatat gcacctgtag aatataatct agagcaaact 60
agttagtcca attatttgtg ttgggcattc aaccaccaaa attatttata ggaaaaggtt 120
aaaccctatt tccctttcat ccgggccctt gcggcggacc gtccgcgaca ccagggtgag 180
ccttggacag gaacactgca aaaacacaag ttaacactac ggatcgtccg atggagaagc 240
gagcaccgtc cgagaccaag cacggaccgt ccggcctcag gcgcgaatcg cccggtcgtt 300
gaaaaaccag aaaaacccga aggtgacggg ttcggtaaaa tgcattttta gcgtccttgc 360
ggatcgtcct gggtgcacgg tcggaccgtc cacgactgct ttatctgaca tttgacgacg 420
cattaaaagc tctatagccg ttactcctga ccgttgtgat ttcagtcgtt gatgtgcagg 480
ggtacggacc gtccgcggtc ggtagaaaat gagcaacgac taggaagtgg ttggaggcta 540
taaatacaag agaaaattcc tggtatgcca tcagatttta tactcatccc ttgtgtgcca 600
ctgagtggca tataagtata tttttttgtg tctatgacat gtggggccag tggcatacaa 660
ggaatgagta tatttttcag tggcatacag ggaattggcc ctaaatacaa ccccaaccac 720
ctccattcaa atgatccaag cactccactc attcacattc aatacaggag ctagcaatac 780
attccaagac acactcaaag ctttcaatct ctcaaagtcc cacaatttag acaagtgatc 840
attagtgctt agtgacttga gagagtgtga tctatgtgtt atttgtcgct cttgttgctt 900
ggctttcaca attgggcttt cttcatctct ttctcaacct tctaagtgaa ttataaagca 960
agcaagagac acctaatttt gtggtgatcc ttgtggggtc ttagtgaccc gtgtgattaa 1020
gaagaagcac tcgaccggtc taagtgaccg actgagagag ggaaagggtt ggaatagacc 1080
cggactttgt ggcctcctta acggggacta ggttctttgg aatcaaacct cggtaaacaa 1140
atcgctgtgt ttatttgtgt tgattttcac tcgatttgtt tcccctccct tcctctctct 1200
aaaattccct tgctcatatt gttgtgagtt ggctctcaaa gttatctgca ttgattgggc 1260
aactacttgc aaggataact atcttccgca ctccgaatta tttctgacat taaccccggg 1320
cataatgtgt gttttaagtg tataattttc atgtttcgcc tatttacccc cctctaggcg 1380
actttcaaat gttctccttc acttgtgatg tctacaacca taatcagctc aacatttgga 1440
ctatcaccct tgaacactta tgttgaactt taaaagttgt gcactaagca cttgtccaac 1500
acttaacaca cttgtcagtc ctttaattgg gttgtcatct aaaccaccaa aaaccacaaa 1560
gagatctttc accggggtcc gtggttcatg gccgtactgc tcggtctcaa gatttttatt 1620
ataaaatcac tagagctcta ctatttatgg ttcggtgtgc catcgaaccg tctccaacgg 1680
gtacatccaa cgagcgccag cacaccaacg actagttgac gtggtctacg gtccagaggc 1740
tcatcagact tgtcaggagg ctcgtcgact ggtctggtgc cacacccgtc gacttgggat 1800
cgaacaagaa tgatggcaag aggacgaagc gcatcaagaa gatcatctac tacgactcct 1860
cttacccttc acacaaggac gacgattcca cctcctccaa gaaaaatacg gttaaacaag 1920
gttactctaa gacatctttt atttattctc gcattcctta caatttcaat gctcatttgc 1980
tttctattca tcttggcaag cctgctcgct ttgatgggag gactattctt ggtggagcca 2040
taaaatgcgt agccatattt ttttgctcca ccctagcatt tgggatgtcg tagacaatgt 2100
aatgcaattg ctaggtagcg atgataaaaa ttataatact attattgccc aataatctat 2160
tcataatagc gcccaagcta aagaggtgcg ctaagggatc ctttttatag cccaaagagg 2220
tccataggcg ttgctccttc cttctaaaca tcgatgaaat tgtgttgtct gcgaggacat 2280
caaaccgggt ctgtgcacac cttctccggg atctgatttg gtgctttcct taactgattc 2340
acagcggacc gatccgatgc accaccggac cgtctgccac atcagctagt cgttggcctt 2400
caaactccac cgggaagtag ccgttggagg cggtccggtg cgccattgga ccgtgcaaag 2460
tgaagggtcg atgattttta taaataaaat ctcgagacct caaagttcag ctctgtaggc 2520
ggtccggtgc acatggacct ggttcgatgc attaggtcgc ctaacactaa gttctatgcc 2580
ctgcggactt gatccggtgc accaccagac gagtccgatg aggcctagaa caacccaagt 2640
aaggctgttt tgagcctaac ttcttcaaat ccttttggct attcttggga gctttccaac 2700
aacttagaca aacataatta gcacatattc caattgatta ggtgtggaga actcaccttt 2760
tactttgtcg ttcaccatga tttgcatttt ggcttaatct aagtattcga accacttttc 2820
tcacaggata gagttagagt tcaaataaag tgctaaacac atagtattag acacatgcaa 2880
cttatctaag taatcaaacc tcatgatttt acccttttgt ccaaagctgc acactttagc 2940
cttcatttta gttctttagg atctagtact ttcaaattga cttcaagtgc ttgtgctcgt 3000
actcatatca aattagttag tccatgttgt tgtgctaaac acttaatcac taaaacatgt 3060
agaaatggtt atctaacaca tttttctttc ataagtaaaa caggagattt atattgtaga 3120
tgttattgtt tgttgatgaa atttgaaata agagatataa gagcaactcg aaaagcctag 3180
ctaaatcgat ttgtatcggt aaaaatagaa aactgatgat taaaatagga tccaacaaac 3240
tctctttgct cctctctatg ctatcctgct cagcatcacg tcgaggtctc tagccatatt 3300
tgctgagctc acctgcctcg ccatcttcat tctctcgtgc atcctcaccg tccctgcgcc 3360
ttgccgtcgt tgcagctcac tgtcccacgc cctgctgtcg ccatgcctcg ccgaccctgc 3420
ggctcacctt accgcgcctc gtcatcgtcg cggctcgccg tctcgtgcct cgccaacccc 3480
gctccttgac gtcctcacgg cttacagtcc ccatcgtcct cgtggctcca cgcctcgcca 3540
tatttgcggt tcactgtcca cgtgccctgc catcctagag cccgacatcg atatgtcaaa 3600
tgagacagga gaaaaagaaa tgaacatgtg attataatca gtgatttgaa tattgataga 3660
taagatttga agagtctgtt gtgtcatatc acctttttat gaaactcttt attttttaga 3720
gtttttataa aactctaaat ttagctaaaa ttatatctag tcttttagag ttactctaac 3780
aagagatgag atagcgagct ggcgagctgc tggagacagc cgagagtaga gatagtagag 3840
gagactagaa actccattag gcctagccca gagtcagcta gggatgccgc ccgatccact 3900
acgtactgaa agcgatcccg gcccatgaac gctagtgggt tgtaattcgg cttacccaag 3960
tacccagccg ttcctccaca cctctgcact acccgaaacc cacgcccaac ggccgtttcc 4020
cgcaccccct atccgggaag gaagaaagcc agaactcacc cctgcttcgt ctggcggcgc 4080
cgtttcccgc acgcgatgcc gtcgacggcg gaggatttcc cggcgataag gaagctgggg 4140
aagctcttcc ggcttaccga agtgtacctc tggtaagtct cctgtctccc ctacccgctt 4200
ctcagcgtag ggtttgccgt ttgcgaggag tacgtctcct caaactactc tctcttcctg 4260
cagggacgat tcgtatggcg ctggacctca cgatggacag aagaacgggc gctcggcgga 4320
ggctgctctc gtggtacggt ctctgacccg ctttggtgta gctcactctt aagctttctg 4380
agttgggggt gcgcttgtgc ttcgactagt agatggctaa tttcgtcggg ctactggtaa 4440
tttcttggta tctgcattgt cgagaaagaa ggcccgacgc ataatttcat gcttgcccaa 4500
gagtctactt aacacaagga attggttttg tggcgtggtt tgtgcattgc gcccaaactg 4560
tagcctgtac aaaatgttaa tcgtcgcgtg cattttaaac aaagttttgt attatacgac 4620
aaaataccct cggcacattt gttacagact accgataagt gcatacctat ttctcctagt 4680
tctatcagga aataatcctg gacctcgaaa tgacagcctc gtctggctag aaccctacta 4740
aacattttga gtgatcactt ttcattactc attttcttga tgaaagcaca ttactgacat 4800
ggaagtttgc tacataagac ataacacttc cttgtagtgc tttatttaat tattgaccga 4860
tgatcttttt ggaaaattaa gctgtattaa acaattgtag cttcggtgat gattgttgga 4920
ttaagcatta gtctgctgca gtcctcttct tgattctgat atgacagtta tttgttgatt 4980
aaaataatga tggtttgctt tacacttcga tctcctttga gaggaaaaca tgtgaaggtg 5040
tggactagat catgtataga ccaacagcat tatcttatta aaacacttct aaataactta 5100
gcaatttcat aaccattttt acacttctga ggaattcatc ttgtcgtgaa agagtcaatt 5160
aacttagctg cttagcagac tgtgtcaagc ttattacttg tatgttgtgc cctacaaatt 5220
actatgaggt ttataatgta catagcaatt tgacgacctt caacttttca ggactctcat 5280
acagataaaa catgcaatga agcatccaat agcactgaca aaggttagat gtatttttct 5340
tgtattctag tatcttcctt ggtcaatttt ctttacagag gatgttacaa tgtactctac 5400
tttttttgtg tggaaacaac ccactagaga aaaaaaatca cttcatttga gaaatcttaa 5460
gaatctgaac tctgaagctc agcatgcttc cacaccacca cttttcaggc cactgtctct 5520
tcattggagt aaatgacgct tcttttagag agaaagagag gggggggggt ctgtttataa 5580
ttgaatcaag agattttatt ggtcacctga tttctgttgc atgacgtggg acctggatag 5640
acttcagatt tgccttagtt gataagttca ccggcactag tgaaagaaaa gtatatggta 5700
caggtactct tatgaacagg caaccactta gcaattcagc atctaataga gaaggaccaa 5760
gtcttcaact aaagtcacaa tataccttta gtactattag aggggctgac cctccttgtc 5820
tagtgcttgt gagatcatag gagatggggc gtggtggtta agattgtgga tcatattcgc 5880
caagctccca gggtcaatgt gattgaggga tgtggtatgc tacttgtgaa agggttcaaa 5940
aaggccagga tgacattgtt cctacattcc tggaatgggg atgacacccc caggacaagg 6000
aaggtggcac agttccacgt tcctggtgac atgttgtgta tcaaatggga ggccaaatca 6060
ccacgggatt actctaggaa gggaagatga tgttgataat ttgagtcatt gttgcaacat 6120
ctgttcatgg tttcatgcct cattttataa gtcatattgc ccacacataa cattgtaata 6180
gtaaaatcaa caccagttat tttacgtttt cccttgtatg tcaaccgatt tcttactgtg 6240
tatatgatct gtctatcaat aggccatttc tttgttgaag atttggaatt ggtcaatctc 6300
atgggttctt tggggcttcc tgtttcattc agcacaagta aagtggtcag ttgctcacat 6360
agtgtgacac catgttactg ttccctaccg gttccattgc ttaattcctt tatgcattgc 6420
agaacaagaa cacatgcaac aagggaaaga aaaaaggaag acaagcaccg ctcaaagcag 6480
caaacactca aatcaatgat gctgtgagga tatgtatcaa tactgaagat agagaaaatt 6540
ctgttgaatc attggatgct atggagcaaa cgcactcatg caatttattt gtgacaccac 6600
tgggtcaaaa tgaaccctcc cgtgatgaca ctgacaagag gcttagggaa gacagctctt 6660
gtgttgaaga acaagaagag tctggctgta gcaccatcta ctctgctggc aaagcccctg 6720
gctgtgatgc taaaaatcat ctcactgaac ttggggcttt tgagctttct gataacttgg 6780
ccaactcagc aaaagaagaa tactcaattc aagaaaatca agcttatgaa agtgtgttgc 6840
tagattctga agagatgtca aggaatgact gtgttgatga tgaatctaca cattcctgtg 6900
ttggcattta tcaggatgaa agagtgtcca caaggggaga tcaaacatct gaagaaactc 6960
tatcagtacc ccatgattac aatgatgttg gcagagaagc tagtctaagt ttggcagagc 7020
catcatctat tgatgagcat gcacaaagct ctgccaacaa cttttactat gactatggtg 7080
aatggagggt tatctgggat ccattctata atcggtatta tttttacaac atccagacac 7140
aagagtccac atggtgtcct cctgaaggac tggaggattt tgcatcatat tgtagcccag 7200
ataccactaa agagctagct gaactgggat ctcagtgttc aagcatggca ccacaagaga 7260
acagtaaaaa ccctagtctc gtcctttgca gttgacatta cgaatagtta tatgcactac 7320
gataaaaact ttctacaata tgtaacactt gagcatgtgg caatgggtgt aaacatttaa 7380
taataaggta gtgaaatcca ttacacacag tattgaattt tgcactacaa atgctgaagg 7440
agaaacctaa attgtcaatg ctttttggtg acattaatta ttgccattga tttcctgctt 7500
gtaggtgctt catttatctg tctccaattt actcatatgc tagcttcttg tttgggacta 7560
aaggctttgc tgttgtttta gtatgtcaca catttctctt taatctcacc atcacagatc 7620
tggctactca tgtcaatcat ttagaagcac aggagcaaga tcactgcatt catgatttat 7680
ctgacattcc tgttgaaaag ccaatatatc aaaggtaggg aataccaaac tgtacaatgt 7740
tgaacaagtt attgtttttt tttgttaatt ctgttcatct atgcagtatg ataactacct 7800
ctgacaaagc acagcacact gaaaataagt acagcgattc aacaactact gtgttagaga 7860
tgaaccagga agttgctagc accaaaacga aaaagagagt aaggagatct cgatcgtgta 7920
aggcgataat atatggcatc tgctttctag gagtttgttc ctgttacaat tttaggttgc 7980
gcatttacac aatagtttct tggtttcttt gagcaaatgc agctttgcat gactgctaca 8040
ttgcctactt atgtctaggt aacttttctt tgcaaactgc aaagttatgt ctaggtaact 8100
atgccttcta gaaaacctcc ttgttagcta tgtattagtg agacttgcct aatatttatt 8160
ttcttgtggt ccgttcttgt gctctttgta catatttgcc aataaccatt ttaattgttc 8220
tacagatcat tcatgccaag acatggcagg gaacgtctct aatgacatca tcaagtactg 8280
ggctcagcgg tattcacttt tctcactttt tgatagtggt ataaagatgg atgaagtagg 8340
gtggttttca gttacgccag agccaattgc aaagcatcat gcatctcgtg tgggtgcggg 8400
agtaatgatt gattgtttca caggagttgg tggaaatgcc atccaatttg ccaaaaagta 8460
cgtcaatgtt atcttgcaat tgagttatgt gatggtctaa tgtatcattt gcttgaacac 8520
ttcctgttta gtagcaactg ttatttttct tatgtcacga gaatgcaatg gctatatcac 8580
cttaagcagt atgctatgtc cactgtccag tttaactaag gcatctgctt ccagtaatat 8640
gcaaggctct tcttactttt gctgttattt aatatatgga agtgtcctta cggaggtgtt 8700
attgtggaca ttttgagcat gttcatcatg tcacttgagt tagtagagcc agccttagtt 8760
gtttgcagtg taggtggatt tattttatgt tatcaatgtt tcttctacag tactaagact 8820
attgttccac attaactatg tctccttttc caggtgcaag catgtaattg cagttgatat 8880
tgatccacaa aagattgatt gcgcgcatca taatgcatcc atttatggag taaatgatca 8940
catagatttc attgtaggtg attttataca tatagctcct catctgaagg taatgccttt 9000
ttcttggaat tattactttt aagtttctca acacgtcact tctattagct atatgttttt 9060
gtagctgttt gcgagagtga atttattgtt gacattgttc tcatttgccc acccatttta 9120
ggataggggc ttggtactac aaatatcttg atacttcaag tcctacaaaa agaaatttat 9180
gtttcatatt ttttccattt gaacgtcgag attttatggt cccatggagt tctccctatt 9240
tttcgatgat gcccatcttt tggcagtacc ttctttgtgt acacaataaa tgggaggata 9300
ttttctgcag ggagaaactg ctttcatgtc gcctccttgg ggtggccctg actatgccaa 9360
agttgatgtt tatgatatga aaagcatgct tattccttgt gatgggttag ttccttgttt 9420
ctattttaag agagtaattt ctttcagttt gcactcactg atgtttactt actttgtgag 9480
taaaacgcac cagagatcca ttaaccttta aggaggtgtt atctatgtcc atcaacactc 9540
aaactgcatt tttgggttcc taaacttttt aagtgattca ccggagttcc gtaccccttc 9600
gtttatattt gtattttgca gaaacctcac tctgttttat ttctccttcg catgtagggg 9660
tggtaatgga tcacgattca aacgtttctc cacgattcgt ttgagccctt aattaatttt 9720
agtacaaaaa taaatagaaa tagagataga gcctgatcct aatatgattt gatcctcaaa 9780
ttttatagcg tagaatttag agcccattac catttaccac ccctattcac atgcctaccc 9840
ctctccatct tctggattga atgttccaac ctaatttaca ctcgtagttt ctttgatctg 9900
ccaatcaaat ccagagccta attgctataa cattagaacg aacacgccat attaccagaa 9960
tactcgatgc agatatggat agaagcgagg cgctaagcgc agccagcctt ggcttcttgc 10020
tctgcaggcc gatcagggcg ccagccaaag ccaaccatgc gcgcacgtga ctgcaatgct 10080
actctctctt cgcctttgcc atcgtcgtcg caggatgtta cgttgtgctt atgctggctc 10140
ccacgagtgc cgccgcccag agcgagctga gcgcacgcag ccactgcttg tggttcacga 10200
gcgtgagcat gccctcacca cctgctgctg cgcccttgct gcttgcttgc ttgccggtgg 10260
tgtacattat ggacggatta aattgaatgg atttacctgt tccagaaaaa gatctgatcg 10320
acgatgggat gctatcttgt atggctccgg atcaatgaag attaatggaa caaccaatcg 10380
aaggctcaga gcaggctagt tggtgcccgg aagactctgg ccagaagatg gaaatgggta 10440
agcgtgtgaa ggaaaaaaga aatagagggg gatttctaca aaaaacaaac ataatgaaga 10500
ggtatggatt tcaggtgaac cacttaaaaa ataaaaaggg catacccagt gccgtaggct 10560
tcccgcactg tgcggggtcg tctggggaag ggtatcttta agcgtcaagt cttacccgca 10620
taatatgcag aggctggggc tcgaacccgg gacctttcgg ttatagacgg taggctctac 10680
cgccgcacca agcccgccct tgaaccattt aaaaaattta ggactcaaaa atacagtttg 10740
acagttgatg gacctagatg acacctcctt aaaaaattta atggacctat ggtgcatttt 10800
aatcttttgt tgatcgacta tactcaatgt tgaactcttt aggtactctc tttttaaact 10860
cggaaccatg atagcttcaa gagtagtgat gtttcttcct cgcaacattg acctaaacca 10920
attggcggac atgtccttgt ctgtggatcc cccgtgggca gttgaggtaa gcccattttt 10980
gctgattttg tgccaagctg acgtttccta tagatgtcac agtggtctct ctctctgcag 11040
gtcgagaaga acttcctcaa cggaaagctg aaagccataa cagcttactt tgaagaacag 11100
gatcgttgaa ccaagcatcg gcgctggtga tacaaatcat cttgttagct atgactcacg 11160
acaaattttt gtggtgaccc taaacagaac ctttgtgttc ggagacagaa agaagcggtt 11220
tatcatcttc accgagcata gataatttat ttgcagagat gagtcattgg tatcatacaa 11280
aagcagctca gcttatctca attcacagca agtgaaactg tcgaaggaaa actacaaggc 11340
tgacagtcga acgcgtggga gttagcttaa ttttgcctta tgataagcaa gcatgcttcc 11400
tggtttattt catacagcta ctagtagttt cagctgcaac agttgtgcgt tggtgtgcgt 11460
gtgattctca catatctggt cctgcggatg tgagtgatgc aaatgtatgt gtcatcatcc 11520
catgtaaggg tttgtttgtt tgctctcaat ctatgtagat tgagtgggat taagtgagtt 11580
taaatctcag acaagtcaaa aaaaaatgtt ttcaatctca tccaatccac atatgatagt 11640
aatacccgag taaggcttag atgtaatagt tggaataaga aaaacaagtc agccattttg 11700
aagttttgtc cttggagttc tattaaaagg cattactgat aaatctccaa cagatttgca 11760
gttgaagcaa catgtgaaac atatttatca tgttaaaaca atttgcctta gtattcgatt 11820
atgccatgaa atctgacatt tccttacaca tcccagttta tcattgtcaa ctgtctttag 11880
gaatgtattg tatctgctgt ttttacttgt atatgtatgt tattttttgt cgttgtatgt 11940
atatgtttta ttataaacat ggccactaag gttgttctat tcgttaaaat aacacagatc 12000
tataaacgac taaacaagct tcttgggata aagaatcata tggaggctgg attttcgagg 12060
agtctggtgc actgttttgc taatgatcag cccccccccc ccccccctct aaaaataaag 12120
aaaatactgg atttcctgtt catttattac attcatatgt aaatgcttct gtccttttct 12180
atatctgggc tggacttttt gtgtgctcgt cactcaagtt ggttagtgtg gttaatttta 12240
ttatgctccg tgctctttcc taccgaactt ggtctttgtt agtatcatta tcagtcagtt 12300
atattttctc ctcttgatgc ttcatctaat ctatttttgc aaagttgtca tgttatgtac 12360
tatatgatct tttacaaggt ttttgacttt tcaaattatt gtgtcgtata ttatttgtac 12420
tcagattgtg cttacaactt tagtttatct atactttaag gggtgtttgg tttctatggg 12480
ctaatttata atcccttcat tttattctat tttcgtacct aaattgtcaa gcacgaaaac 12540
gaaaataaag ttttaacttt tatatttagc agtttataca ctaaaataga ataaaataga 12600
tgaagtaaaa attagtcctc agaaaccaaa catctcctaa atgtctagta atagtcgcct 12660
gaactgtaga gcgcccaaca cgcgccaccc tgatttggtg tcttaaaatg gcatgtgtat 12720
ataggtggaa tgggtttgac gagactgtaa ctactttttc ttaattaaat tatagatgga 12780
cttaactttt ctatatgcat tttaaatata tttttctata tttttggtgg gctgagttac 12840
agtttatgtc aatataaatt acaacagacc gaatctaaaa ttttattata aaatgtatgc 12900
accaaccgtt gactaaaaag ataaaatttg gacacctaca ttttagcaag tcacctgcta 12960
atatatatct atactaggta agtgtccgtg cgttgcaacg aaaacatata ataatacgat 13020
aacttatata taaaatatgt gttatactgt tatgagaaaa agtttcacct gtcctatttt 13080
tatcaatatg acaacagagg atcaatataa ggccttggca tggcttcaga agttcagatt 13140
aacgaattat ggagcaagag caactatttc tggtgtttca gtagaaagaa tggggatatg 13200
tgtttatctc tctcatacat gttacacaat gtgctataga atgacacctc taggccgctg 13260
ctacaactac agagaataaa ttatggatca tgggtgccct actaagttag ctacacgtaa 13320
aatctggggc gattgacccc ctaatgcttc atcttggaga tctcactaaa atacaccttc 13380
cgcacaaacc gagccttgat aaagctcgcg atcttcgtgt cctgatcttt agtgcagaca 13440
actgcaaggg aaattattgt gccggtcagc aagagtggaa aatgtcagca gaaacacaag 13500
aatggaaaat tatatgatgt gcaagtagga cggcacccta tttaactaaa ggtgtgtttg 13560
gttcagtttt ctgaaccaat tcgttgccaa aaaatctaaa atctcacaca aacggtacaa 13620
catcagaata gatttttaaa agtttataga tttctcaagt tcaattcaaa atcaccatct 13680
accccaaatt tttcagatta ctattattca tgttacaact atcactcttg tagtatctac 13740
cattgatagt tgttttaatc aaatatattt tagctttctc acaatcctca gctcgaaaca 13800
gattttcatg gctcacagtt ggattcatat tttcataaat ctataggtgt gaaccaaata 13860
gaccctaaaa catcatgttg taatgttcca aaaaaatcat cacaaaaaac atgtatgcaa 13920
cacacctaag tgcaacaaca ctaacctgca ggagcgttgg cgtaggctgg actcttgtgt 13980
gtctgcaatg tggaaacctg cattgtaaat ggttaggtaa atgcttcgct taaaaatggc 14040
agtaaatgct tcactaaaaa tgcttccacg tgctcatgat caagtaggtt ttatgttcaa 14100
tctgcagttc tacacaagtg cgattcattt tgatactcat ttctatttac tttcagagct 14160
cgtgctaact gtcataaagt gcaatgcatc tatgactgcc aattgatatt gtgctcctgc 14220
cataaagtgc actgcatcgt gctaactgta cactaatctg tgaggatcta agaccaatat 14280
ttgtttacgt tttctccttt agtatcctat aaaaacaaca cgcctagaca acccaaaaaa 14340
tgtgtcccaa taggaatatc agattctgac gctggcagca atctcgaatg ataatatttt 14400
ttccaaacaa gcgaggtccc taaatagaag cggcaacaga taaaaactaa agataaaaac 14460
taaagagtac agatgattgg catcacatcg ggaatgaaat atgcctaaca tatcaatttg 14520
catattagat tatttgctga gaacaataac gaaaacatat ttagttgttc atcacaagtt 14580
accttagatt ttgctgttca aggtcctttg ggtcttcttt ctgctagaac atacaagggt 14640
atttcagatt tgcaaacaag gaaaagcaag aacttcaatg atacatcatt gtaaaaccaa 14700
gtttccgatt taaataaaga tgatgcttgc ggtcaacaca ttcacaaatg taaatgtgtg 14760
aaatcgttca aacataaggc ttatgtggtc atgctcaggt agtatgtaca gacctaaaaa 14820
caaggtatat gacaacagta ccagccacta aacacacatg gttaatcact aaaacaattc 14880
tccgattaac caggaaaact atagcagcca ctagaactat acaggtttct accagtaatt 14940
gcttcactaa aaaatgcccc catgtgtaaa ttttcaggtg gtttgtacag acataaaaac 15000
aagggtatag gactacttct tgtgctaaaa taaaagctgg cactaaacag tgtatccagt 15060
tcatcaggaa aacagtttta gtgattaatc actaaaacaa tacccatggt gcaaattatc 15120
tgattaacca tgtacatacc agtaattgct tcataaaaaa tgcccccaat ttgctcatgt 15180
ttaaggaata tgtatagaac taaaaacaag ggtatatgat aacaatacca gccactaaac 15240
acacatggtt aatcactaaa acaattctct gacaactcct ataaaaagat agcaaccacc 15300
agaaataaac cgcccacgac atccctaatt gtagtcacta aaaactggta gcagtaattg 15360
gttcactaaa aaatgaccat aatcacgaaa actataacag tcaccagaac tatataggtt 15420
tcattagtaa ttgcttcact aaaaaatgtc cccatgtgta aattttcatg tcgtatgtat 15480
agacctaaaa acaagggtat atgactactt gctatgctaa aacaatagct agcactaaac 15540
agtgtattca gttaatcatc caaacaattt tgtgattaat cactaaaaca atacccatgg 15600
agcaaattat atgagaataa gatctcgtcg ttcctattgt gaagaatata ctactacctc 15660
cagtttcaaa ttacaatttc aaattacaag ttgtttagaa catccacaag gtaattgcga 15720
agaatatact aattgctcta gtttcaaatt agaagttgtt tagagaaaag gtgttcctta 15780
atagtctagt tttagaagct acatccaact ggtaaacata aattgcagaa accttttatg 15840
tggaagcctc cgtcattgag tctgtcccct ttagctgtaa gtagttttct aaatattgtt 15900
agtcaggctt agttgtttga gactctgttt ccattcgtga ccatgggaac tgtgaaatgt 15960
gtagaagatg ctcatgctca tgcatatgca tcgaattgtt ttgtaaagtc atcttaatgc 16020
tcaaacagtt tttttatctg ccccagctgt acactgcttt ctgaattatg tcatttaggc 16080
ttagctgtcc gagataattt catttgtgat gaaggtaacc ggagcatctg tccttttgtt 16140
ttaaacataa atattttgat agcttaactt gtgcgtcatt ttatcatgta ctaacatggt 16200
atatagatgg cacttagcag taacattcct gacttatgtg attgtcctgt tagaatgctt 16260
ctgcaatata atgggcttat gctaatctgt ttggaatccc atgatgaata acaattatgg 16320
atgttgggca ttttgtattt ttatgatgta ggcttaacat attttcttct ctgctcagcc 16380
ctgccggtgg agacctctat ttatagctat aatccagcca tatctagtga actaacagat 16440
ctattcctat ctctagtgct tatcccctta acagatttat tttcctatct ctatttcttt 16500
gatgttactt gctgcaggtg ctatccccca ctgatgcatt atatgacaat cactcgaaga 16560
atcagatgct aattaattgt tttatacttg accattgcta attaagtact ccatttttt 16619
<210> 13
<211> 17274
<212> DNA
<213> Zea mays
<400> 13
caaccctttc cctctctcaa acggtcactt agaccgagtg aggcttcttc cttaatctca 60
tgggtcactt agaccccgca aggatcacca cacaattggt gtctcttgcc tcgcttacaa 120
agcacttgag agtaagaagt gagaaagaaa agaaagccaa gccaagcaaa caagagcaac 180
aaagaaacac aaatgatcct ttaacaagtt ctaatgcgct agagttgaat cgagaacttt 240
gagtggatcg atcacttgaa ttgtgtcttt gcagtggagt ctattgctct tgtattgaat 300
gcaatgtgtt gaatgcttgg atggttagag tggaggtggt tgggggtatt tatagccctc 360
aaccaccaaa caactgttgg ggaggggttg ctgtcgatgg gcgcaccgga cagtccggtg 420
cgccagcaac gtcacccaac cgttagggtt cgagcgcaga cgactgttgg agctttgtct 480
tcttgtgcca ccgaacagtc aggtgccgca ccggacaggc actgttcact gtccggtgcg 540
cctctgacgg ctgctctaac ttctgcgcgc actggtcgca cactgtagcg ttcgcaggtg 600
tccgttgcag tcgaccattg tgctggaagc cgttgctccg tttggtgcat cggacagtct 660
ggtggcacac cggacagtct ggtgaattat agcggagtgc ggcctgagaa acccgaaggt 720
ggagagttcg gagttgtacg gtcctggtgc atcggacact gttcggtgcg ccagaccagg 780
gcacctttgg tttctttgtt cctttgcttt tgaaccctaa ctttgatctt ttattggttt 840
gagttgaacc tatatgcacc tgtagaatat aatctagagc aaactagtta gtccaattat 900
ttgtgttggg cattcaacca ccaaaattat ttataggaaa aggttaaacc ctatttccct 960
ttcatccggg cccttgcggc ggaccgtccg cgacaccagg gtgagccttg gacaggaaca 1020
ctgcaaaaac acaagttaac actacggatc gtccgatgga gaagcgagca ccgtccgaga 1080
ccaagcacgg accgtccggc ctcaggcgcg aatcgcccgg tcgttgaaaa accagaaaaa 1140
cccgaaggtg acgggttcgg taaaatgcat ttttagcgtc cttgcggatc gtcctgggtg 1200
cacggtcgga ccgtccacga ctgctttatc tgacatttga cgacgcatta aaagctctat 1260
agccgttact cctgaccgtt gtgatttcag tcgttgatgt gcaggggtac ggaccgtccg 1320
cggtcggtag aaaatgagca acgactagga agtggttgga ggctataaat acaaccccaa 1380
ccacctccat tcaaatgatc caagcactcc actcattcac attcaataca ggagctagca 1440
atacattcca agacacactc aaagctttca atctctcaaa gtcccacaat ttagacaagt 1500
gatcattagt gcttagtgac ttgagagagt gtgatctatg tgttatttgt cgctcttgtt 1560
gcttggcttt cacaattggg ctttcttcat ctctttctca accttctaag tgaattataa 1620
agcaagcaag agacacctaa ttttgtggtg atccttgtgg ggtcttagtg acccgtgtga 1680
ttaagaagaa gcactcgacc ggtctaagtg accgactgag agagggaaag ggttggaata 1740
gacccggact ttgtggcctc cttaacgggg actaggttct ttggaatcaa acctcggtaa 1800
acaaatcgct gtgtttattt gtgttgattt tcactcgatt tgtttcccct cccttcctct 1860
ctctaaaatt cccttgctca tattgttgtg agttggctct caaagttatc tgcattgatt 1920
gggcaactac ttgcaaggat aactatattc cgcactccga attatttctg acattaaccc 1980
cgggcataat gtgtgtttta agtgtataat tttcatgttt cgcctattta cccccctcta 2040
ggcgactttc aaatgttctc cttcacttgt gatgtctaca accataatca gctcaacatt 2100
tggactatca cccttgaaca cttatgttga actttaaaag ttgtgcacta agcacttgtc 2160
caacacttaa cacacttgtc agtcctttaa ttgggttgtc atctaaacca ccaaaaacca 2220
caaagagatc tttcaccggg gtccgtggtt catggccgta ctgctcggtc tcaagatttt 2280
tattataaaa tcactagagc tctactattt atggttcggt gtgccatcga accgtctcca 2340
acgggtacat ccaacgagcg ccagcacacc aacgactagt tgacgtggtc tacggtccag 2400
aggctcatca gacttgtcag gaggctcgtc gactggtctg gtgccacacc cgtcgacttg 2460
ggatcgaaca agaatgatgg caagaggacg aagcgcatca agaagatcat ctactacgac 2520
tcctcttacc cttcacacaa ggacgacgat tccacctcct ccaagaaaaa tacggttaaa 2580
caaggttact ctaagacatc ttttatttat tctcgcattc cttacaattt caatgctcat 2640
ttgctttcta ttcatcttgg caagcctgct cgctttgatg ggaggactat tcttggtgga 2700
gccataaaat gcgtagccat atttttttgc tccaccctag catttgggat gtcgtagaca 2760
atgtaatgca attgctaggt agcgatgata aaaattataa tactattatt gcccaataat 2820
ctattcataa tagcgcccaa gctaaagagg tgcgctaagg gatccttttt atagcccaaa 2880
gaggtccata ggcgttgctc cttccttcta aacatcgatg aaattgtgtt gtctgcgagg 2940
acatcaaacc gggtctgtgc acaccttctc cgggatctga tttggtgctt tccttaactg 3000
attcacagcg gaccgatccg atgcaccacc ggaccgtctg ccacatcagc tagtcgttgg 3060
ccttcaaact ccaccgggaa gtagccgttg gaggcggtcc ggtgcgccat tggaccgtgc 3120
aaagtgaagg gtcgatgatt tttataaata aaatctcgag acctcaaagt tcagctctgt 3180
aggcggtccg gtgcacatgg acctggttcg atgcattagg tcgcctaaca ctaagttcta 3240
tgccctgcgg acttgatccg gtgcaccacc agacgagtcc gatgaggcct agaacaaccc 3300
aagtaaggct gttttgagcc taacttcttc aaatcctttt ggctattctt gggagctttc 3360
caacaactta gacaaacata attagcacat attccaattg attaggtgtg gagaactcac 3420
cttttacttt gtcgttcacc atgatttgca ttttggctta atctaagtat tcgaaccact 3480
tttctcacag gatagagtta gagttcaaat aaagtgctaa acacatagta ttagacacat 3540
gcaacttatc taagtaatca aacctcatga ttttaccctt ttgtccaaag ctgcacactt 3600
tagccttcat tttagttctt taggatctag tactttcaaa ttgacttcaa gtgcttgtgc 3660
tcgtactcat atcaaattag ttagtccatg ttgttgtgct aaacacttaa tcactaaaac 3720
atgtagaaat ggttatctaa cacatttttc tttcataagt aaaacaggag atttatattg 3780
tagatgttat tgtttgttga tgaaatttga aataagagat ataagagcaa ctcgaaaagc 3840
ctagctaaat cgatttgtat cggtaaaaat agaaaactga tgattaaaat aggatccaac 3900
aaactctctt tgctcctctc tatgctatcc tgctcagcat cacgtcgagg tctctagcca 3960
tatttgctga gctcacctgc ctcgccatct tcattctctc gtgcatcctc accgtccctg 4020
cgccttgccg tcgttgcagc tcactgtccc acgccctgct gtcgccatgc ctcgccgacc 4080
ctgcggctca ccttaccgcg cctcgtcatc gtcgcggctc gccgtctcgt gcctcgccaa 4140
ccccgctcct tgacgtcctc acggcttaca gtccccatcg tcctcgtggc tccacgcctc 4200
gccatatttg cggttcactg tccacgtgcc ctgccatcct agagcccgac atcgatatgt 4260
caaatgagac aggagaaaaa gaaatgaaca tgtgattata atcagtgatt tgaatattga 4320
tagataagat ttgaagagtc tgttgtgtca tatcaccttt ttatgaaact ctttattttt 4380
tagagttttt ataaaactct aaatttagct aaaattatat ctagtctttt agagttactc 4440
taacaagaga tgagatagcg agctggcgag ctgctggaga cagccgagag tagagatagt 4500
agaggagact agaaactcca ttaggcctag cccagagtca gctagggatg ccgcccgatc 4560
cactacgtac tgaaagcgat cccggcccat gaacgctagt gggttgtaat tcggcttacc 4620
caagtaccca gccgttcctc cacacctctg cactacccga aacccacgcc caacggccgt 4680
ttcccgcacc ccctatccgg gaaggaagaa agccagaact cacccctgct tcgtctggcg 4740
gcgccgtttc ccgcacgcga tgccgtcgac ggcggaggat ttcccggcga taaggaagct 4800
ggggaagctc ttccggctta ccgaagtgta cctctggtaa gtctcctgtc tcccctaccc 4860
gcttctcagc gtagggtttg ccgtttgcga ggagtacgtc tcctcaaact actctctctt 4920
cctgcaggga cgattcgtat ggcgctggac ctcacgatgg acagaagaac gggcgctcgg 4980
cggaggctgc tctcgtggta cggtctctga cccgctttgg tgtagctcac tcttaagctt 5040
tctgagttgg gggtgcgctt gtgcttcgac tagtagatgg ctaatttcgt cgggctactg 5100
gtaatttctt ggtatctgca ttgtcgagaa agaaggcccg acgcataatt tcatgcttgc 5160
ccaagagtct acttaacaca aggaattggt tttgtggcgt ggtttgtgca ttgcgcccaa 5220
actgtagcct gtacaaaatg ttaatcgtcg cgtgcatttt aaacaaagtt ttgtattata 5280
cgacaaaata ccctcggcac atttgttaca gactaccgat aagtgcatac ctatttctcc 5340
tagttctatc aggaaataat cctggacctc gaaatgacag cctcgtctgg ctagaaccct 5400
actaaacatt ttgagtgatc acttttcatt actcattttc ttgatgaaag cacattactg 5460
acatggaagt ttgctacata agacataaca cttccttgta gtgctttatt taattattga 5520
ccgatgatct ttttggaaaa ttaagctgta ttaaacaatt gtagcttcgg tgatgattgt 5580
tggattaagc attagtctgc tgcagtcctc ttcttgattc tgatatgaca gttatttgtt 5640
gattaaaata atgatggttt gctttacact tcgatctcct ttgagaggaa aacatgtgaa 5700
ggtgtggact agatcatgta tagaccaaca gcattatctt attaaaacac ttctaaataa 5760
cttagcaatt tcataaccat ttttacactt ctgaggaatt catcttgtcg tgaaagagtc 5820
aattaactta gctgcttagc agactgtgtc aagcttatta cttgtatgtt gtgccctaca 5880
aattactatg aggtttataa tgtacatagc aatttgacga ccttcaactt ttcaggactc 5940
tcatacagat aaaacatgca atgaagcatc caatagcact gacaaaggtt agatgtattt 6000
ttcttgtatt ctagtatctt ccttggtcaa ttttctttac agaggatgtt acaatgtact 6060
ctactttttt tgtgtggaaa caacccacta gagaaaaaaa atcacttcat ttgagaaatc 6120
ttaagaatct gaactctgaa gctcagcatg cttccacacc accacttttc aggccactgt 6180
ctcttcattg gagtaaatga cgcttctttt agagagaaag agaggggggg ggggtctgtt 6240
tataattgaa tcaagagatt ttattggtca cctgatttct gttgcatgac gtgggacctg 6300
gatagacttc agatttgcct tagttgataa gttcaccggc actagtgaaa gaaaagtata 6360
tggtacaggt actcttatga acaggcaacc acttagcaat tcagcatcta atagagaagg 6420
accaagtctt caactaaagt cacaatatac ctttagtact attagagggg ctgaccctcc 6480
ttgtctagtg cttgtgagat cataggagat ggggcgtggt ggttaagatt gtggatcata 6540
ttcgccaagc tcccagggtc aatgtgattg agggatgtgg tatgctactt gtgaaagggt 6600
tcaaaaaggc caggatgaca ttgttcctac attcctggaa tggggatgac acccccagga 6660
caaggaaggt ggcacagttc cacgttcctg gtgacatgtt gtgtatcaaa tgggaggcca 6720
aatcaccacg ggattactct aggaagggaa gatgatgttg ataatttgag tcattgttgc 6780
aacatctgtt catggtttca tgcctcattt tataagtcat attgcccaca cataacattg 6840
taatagtaaa atcaacacca gttattttac gttttccctt gtatgtcaac cgatttctta 6900
ctgtgtatat gatctgtcta tcaataggcc atttctttgt tgaagatttg gaattggtca 6960
atctcatggg ttctttgggg cttcctgttt cattcagcac aagtaaagtg gtcagttgct 7020
cacatagtgt gacaccatgt tactgttccc taccggttcc attgcttaat tcctttatgc 7080
attgcagaac aagaacacat gcaacaaggg aaagaaaaaa ggaagacaag caccgctcaa 7140
agcagcaaac actcaaatca atgatgctgt gaggatatgt atcaatactg aagatagaga 7200
aaattctgtt gaatcattgg atgctatgga gcaaacgcac tcatgcaatt tatttgtgac 7260
accactgggt caaaatgaac cctcccgtga tgacactgac aagaggctta gggaagacag 7320
ctcttgtgtt gaagaacaag aagagtctgg ctgtagcacc atctactctg ctggcaaagc 7380
ccctggctgt gatgctaaaa atcatctcac tgaacttggg gcttttgagc tttctgataa 7440
cttggccaac tcagcaaaag aagaatactc aattcaagaa aatcaagctt atgaaagtgt 7500
gttgctagat tctgaagaga tgtcaaggaa tgactgtgtt gatgatgaat ctacacattc 7560
ctgtgttggc atttatcagg atgaaagagt gtccacaagg ggagatcaaa catctgaaga 7620
aactctatca gtaccccatg attacaatga tgttggcaga gaagctagtc taagtttggc 7680
agagccatca tctattgatg agcatgcaca aagctctgcc aacaactttt actatgacta 7740
tggtgaatgg agggttatct gggatccatt ctataatcgg tattattttt acaacatcca 7800
gacacaagag tccacatggt gtcctcctga aggactggag gattttgcat catattgtag 7860
cccagatacc actaaagagc tagctgaact gggatctcag tgttcaagca tggcaccaca 7920
agagaacagt aaaaacccta gtctcgtcct ttgcagttga cattacgaat agttatatgc 7980
actacgataa aaactttcta caatatgtaa cacttgagca tgtggcaatg ggtgtaaaca 8040
tttaataata aggtagtgaa atccattaca cacagtattg aattttgcac tacaaatgct 8100
gaaggagaaa cctaaattgt caatgctttt tggtgacatt aattattgcc attgatttcc 8160
tgcttgtagg tgcttcattt atctgtctcc aatttactca tatgctagct tcttgtttgg 8220
gactaaaggc tttgctgttg ttttagtatg tcacacattt ctctttaatc tcaccatcac 8280
agatctggct actcatgtca atcatttaga agcacaggag caagatcact gcattcatga 8340
tttatctgac attcctgttg aaaagccaat atatcaaagg tagggaatac caaactgtac 8400
aatgttgaac aagttattgt tttttttgtt aattctgttc atctatgcag tatgataact 8460
acctctgaca aagcacagca cactgaaaat aagtacagcg attcaacaac tactgtgtta 8520
gagatgaacc aggaagttgc tagcaccaaa acgaaaaaga gagtaaggag atctcgatcg 8580
tgtaaggcga taatatatgg catctgcttt ctaggagttt gttcctgtta caattttagg 8640
ttgcgcattt acacaatagt ttcttggttt ctttgagcaa atgcagcttt gcatgactgc 8700
tacattgcct acttatgtct aggtaacttt tctttgcaaa ctgcaaagtt atgtctaggt 8760
aactatgcct tctagaaaac ctccttgtta gctatgtatt agtgagactt gcctaatatt 8820
tattttcttg tggtccgttc ttgtgctctt tgtacatatt tgccaataac cattttaatt 8880
gttctacaga tcattcatgc caagacatgg cagggaacgt ctctaatgac atcatcaagt 8940
actgggctca gcggtattca cttttctcac tttttgatag tggtataaag atggatgaag 9000
tagggtggtt ttcagttacg ccagagccaa ttgcaaagca tcatgcatct cgtgtgggtg 9060
cgggagtaat gattgattgt ttcacaggag ttggtggaaa tgccatccaa tttgccaaaa 9120
agtacgtcaa tgttatcttg caattgagtt atgtgatggt ctaatgtatc atttgcttga 9180
acacttcctg tttagtagca actgttattt ttcttatgtc acgagaatgc aatggctata 9240
tcaccttaag cagtatgcta tgtccactgt ccagtttaac taaggcatct gcttccagta 9300
atatgcaagg ctcttcttac ttttgctgtt atttaatata tggaagtgtc cttacggagg 9360
tgttattgtg gacattttga gcatgttcat catgtcactt gagttagtag agccagcctt 9420
agttgtttgc agtgtaggtg gatttatttt atgttatcaa tgtttcttct acagtactaa 9480
gactattgtt ccacattaac tatgtctcct tttccaggtg caagcatgta attgcagttg 9540
atattgatcc acaaaagatt gattgcgcgc atcataatgc atccatttat ggagtaaatg 9600
atcacataga tttcattgta ggtgatttta tacatatagc tcctcatctg aaggtaatgc 9660
ctttttcttg gaattattac ttttaagttt ctcaacacgt cacttctatt agctatatgt 9720
ttttgtagct gtttgcgaga gtgaatttat tgttgacatt gttctcattt gcccacccat 9780
tttaggatag gggcttggta ctacaaatat cttgatactt caagtcctac aaaaagaaat 9840
ttatgtttca tattttttcc atttgaacgt cgagatttta tggtcccatg gagttctccc 9900
tatttttcga tgatgcccat cttttggcag taccttcttt gtgtacacaa taaatgggag 9960
gatattttct gcagggagaa actgctttca tgtcgcctcc ttggggtggc cctgactatg 10020
ccaaagttga tgtttatgat atgaaaagca tgcttattcc ttgtgatggg ttagttcctt 10080
gtttctattt taagagagta atttctttca gtttgcactc actgatgttt acttactttg 10140
tgagtaaaac gcaccagaga tccattaacc tttaaggagg tgttatctat gtccatcaac 10200
actcaaactg catttttggg ttcctaaact ttttaagtga ttcaccggag ttccgtaccc 10260
cttcgtttat atttgtattt tgcagaaacc tcactctgtt ttatttctcc ttcgcatgta 10320
ggggtggtaa tggatcacga ttcaaacgtt tctccacgat tcgtttgagc ccttaattaa 10380
ttttagtaca aaaataaata gaaatagaga tagagcctga tcctaatatg atttgatcct 10440
caaattttat agcgtagaat ttagagccca ttaccattta ccacccctat tcacatgcct 10500
acccctctcc atcttctgga ttgaatgttc caacctaatt tacactcgta gtttctttga 10560
tctgccaatc aaatccagag cctaattgct ataacattag aacgaacacg ccatattacc 10620
agaatactcg atgcagatat ggatagaagc gaggcgctaa gcgcagccag ccttggcttc 10680
ttgctctgca ggccgatcag ggcgccagcc aaagccaacc atgcgcgcac gtgactgcaa 10740
tgctactctc tcttcgcctt tgccatcgtc gtcgcaggat gttacgttgt gcttatgctg 10800
gctcccacga gtgccgccgc ccagagcgag ctgagcgcac gcagccactg cttgtggttc 10860
acgagcgtga gcatgccctc accacctgct gctgcgccct tgctgcttgc ttgcttgccg 10920
gtggtgtaca ttatggacgg attaaattga atggatttac ctgttccaga aaaagatctg 10980
atcgacgatg ggatgctatc ttgtatggct ccggatcaat gaagattaat ggaacaacca 11040
atcgaaggct cagagcaggc tagttggtgc ccggaagact ctggccagaa gatggaaatg 11100
ggtaagcgtg tgaaggaaaa aagaaataga gggggatttc tacaaaaaac aaacataatg 11160
aagaggtatg gatttcaggt gaaccactta aaaataaaaa gggcataccc agtgccgtag 11220
gcttcccgca ctgtgcgggg tcgtctgggg aagggtatct ttaagcgtca agtcttaccc 11280
gcataatatg cagaggctgg ggctcgaacc cgggaccttt cggttataga cggtaggctc 11340
taccgccgca ccaagcccgc ccttgaacca tttaaaaaat ttaggactca aaaatacagt 11400
ttgacagttg atggacctag atgacacctc cttaaaaatt ttaatggacc tatggtgcat 11460
tttaatcttt tgttgatcga ctatactcaa tgttgaactc tttaggtact ctctttttaa 11520
actcggaacc atgatagctt caagagtagt gatgtttctt cctcgcaaca ttgacctaaa 11580
ccaattggcg gacatgtcct tgtctgtgga tcccccgtgg gcagttgagg taagcccatt 11640
tttgctgatt ttgtgccaag ctgacgtttc ctatagatgt cacagtggtc tctctctctg 11700
caggtcgaga agaacttcct caacggaaag ctgaaagcca taacagctta ctttgaagaa 11760
caggatcgtt gaaccaagca tcggcgctgg tgatacaaat catcttgtta gctatgactc 11820
acgacaattt tttgtggtga ccctaaacag aacctttgtg ttcggagaca gaaagaagcg 11880
gtttatcatc ttcaccgagc atagataatt tatttgcaga gatgagtcat tggtatcata 11940
caaaagcagc tcagcttatc tcaattcaca gcaagtgaaa ctgtcgaagg aaaactacaa 12000
ggctgacagt cgaacgcgtg ggagttagct taattttgcc ttatgataag caagcatgct 12060
tcctggttta tttcatacag ctactagtag tttcagctgc aacagttgtg cgttggtgtg 12120
cgtgtgattc tcacatatct ggtcctgcgg atgtgagtga tgcaaatgta tgtgtcatca 12180
tcccatgttt gtttgtttgc tctcaatcta tgtagattga gtgggattaa gtgagtttaa 12240
atctcagaca agtcaaaaaa aaatgttttc aatctcatcc aatccacata tgatagtaat 12300
acccgagtaa ggcttagatg taatagttgg aataagaaaa acaagtcagc cattttgaag 12360
ttttgtcctt ggagttctat taaaaggcat tactgataaa tctccaacag atttgcagtt 12420
gaagcaacat gtgaaacata tttatcatgt taaaacaatt tgccttagta ttcgattatg 12480
ccatgaaatc tgacatttcc ttacacatcc cagtttatca ttgtcaactg tctttaggaa 12540
tgtattgtat ctgctgtttt tacttgtata tgtatgttat tttttgtcgt tgtatgtata 12600
tgttttatta taaacatggc cactaaggtt gttctattcg ttaaaataac acagatctat 12660
aaacgactaa acaagcttct tgggataaag aatcatatgg aggctggatt ttcgaggagt 12720
ctggtgcact gttttgctaa tgatcagacc cccccccccc ctctaaaaat aaagaaaata 12780
ctggatttcc tgttcattta ttacattcat atgtaaatgc ttctgtcctt ttctatatct 12840
gggctggact ttttgtgtgc tcgtcactca agttggttag tgtggttaat tttattatgc 12900
tccgtgctct ttcctaccga acttggtctt tgttagtatc attatcagtc agttatattt 12960
tctcctcttg atgcttcatc taatctattt ttgcaaagtt gtcatgttat gtactatatg 13020
atcttttaca aggtttttga cttttcaaat tattgtgtcg tatattattt gtactcagat 13080
tgtgcttaca actttagttt atctatactt taaggggtgt ttggtttcta tgagctaatt 13140
tataatccct tcattttatt ctattttcgt acctaaattg tcaagcacga aaacgaaaat 13200
aaagttttaa cttttatatt tagcagttta tacactaaaa tagaataaaa tagatgaagt 13260
aaaaattagt cctcagaaac caaacatctc ctaaatgtct agtaatagtc gcctgaactg 13320
tagagcgccc aacacgcgcc accctgattt ggtgtcttaa aatggcatgt gtatataggt 13380
ggaatgggtt tgacgagact gtaactactt tttcttaatt aaattataga tggacttaac 13440
ttttctatat gcattttaaa tatatttttc tatatttttg gtgggctgag ttacagttta 13500
tgtcaatata aattacaaca gaccgaatct aaaattttat tataaaatgt atgcaccaac 13560
cgttgactaa aaagataaaa tttggacacc tacattttag caagtcacct gctaatatat 13620
atctatacta ggtaagtgtc cgtgcgttgc aacgaaaaca tataataata cgataactta 13680
tatataaaat atgtgttata ctgttatgag aaaaagtttc acctgtccta tttttatcaa 13740
tatgacaaca gaggatcaat ataaggcctt ggcatggctt cagaagttca gattaacgaa 13800
ttatggagca agagcaacta tttctggtgt ttcagtagaa agaatgggga tatgtgttta 13860
tctctctcat acatgttaca caatgtgcta tagaatgaca cctctaggcc gctgctacaa 13920
ctacagagaa taaattatgg atcatgggtg ccctactaag ttagctacac gtaaaatctg 13980
gggcgattga ccccctaatg cttcatcttg gagatctcac taaaatacac cttccgcaca 14040
aaccgagcct tgataaagct cgcgatcttc gtgtcctgat ctttagtgca gacaactgca 14100
agggaaatta ttgtgccggt cagcaagagt ggaaaatgtc agcagaaaca caagaatgga 14160
aaattatatg atgtgcaagt aggacggcac cctatttaac taaaggtgtg tttggttcag 14220
ttttctgaac caattcgttg ccaaaaaatc taaaatctca cacaaacggt acaacatcag 14280
aatagatttt taaaagttta tagatttctc aagttcaatt caaaatcacc atctacccca 14340
aatttttcag attactatta ttcatgttac aactatcact cttgtagtat ctaccattga 14400
tagttgtttt aatcaaatat attttagctt tctcacaatc ctcagctcga aacagatttt 14460
catggctcac agttggattc atattttcat aaatctatag gtgtgaacca aatagaccct 14520
aaaacatcat gttgtaatgt tccaaaaaaa tcatcacaaa aaacatgtat gcaacacacc 14580
taagtgcaac aacactaacc tgcaggagcg ttggcgtagg ctggactctt gtgtgtctgc 14640
aatgtggaaa cctgcattgt aaatggttag gtaaatgctt cgcttaaaaa tggcagtaaa 14700
tgcttcacta aaaatgcttc cacgtgctca tgatcaagta ggttttatgt tcaatctgca 14760
gttctacaca agtgcgattc attttgatac tcatttctat ttactttcag agctcgtgct 14820
aactgtcata aagtgcaatg catctatgac tgccaattga tattgtgctc ctgccataaa 14880
gtgcactgca tcgtgctaac tgtacactaa tctgtgagga tctaagacca atatttgttt 14940
acgttttctc ctttagtatc ctataaaaac aacacgccta gacaacccaa aaaatgtgtc 15000
ccaataggaa tatcagattc tgacgctggc agcaatctcg aatgataata ttttttccaa 15060
acaagcgagg tccctaaata gaagcggcaa cagataaaaa ctaaagataa aaactaaaga 15120
gtacagatga ttggcatcac atcgggaatg aaatatgcct aacatatcaa tttgcatatt 15180
agattatttg ctgagaacaa taacgaaaac atatttagtt gttcatcaca agttacctta 15240
gattttgctg ttcaaggtcc tttgggtctt ctttctgcta gaacatacaa gggtatttca 15300
gatttgcaaa caaggaaaag caagaacttc aatgatacat cattgtaaaa ccaagtttcc 15360
gatttaaata aagatgatgc ttgcggtcaa cacattcaca aatgtaaatg tgtgaaatcg 15420
ttcaaacata aggcttatgt ggtcatgctc aggtagtatg tacagaccta aaaacaaggt 15480
atatgacaac agtaccagcc actaaacaca catggttaat cactaaaaca attctccgat 15540
taaccaggaa aactatagca gccactagaa ctatacaggt ttctaccagt aattgcttca 15600
ctaaaaaatg cccccatgtg taaattttca ggtggtttgt acagacataa aaacaagggt 15660
ataggactac ttcttgtgct aaaataaaag ctggcactaa acagtgtatc cagttcatca 15720
ggaaaacagt tttagtgatt aatcactaaa acaataccca tggtgcaaat tatctgatta 15780
accatgtaca taccagtaat tgcttcataa aaaatgcccc caatttgctc atgtttaagg 15840
aatatgtata gaactaaaaa caagggtata tgataacaat accagccact aaacacacat 15900
ggttaatcac taaaacaatt ctctgacaac tcctataaaa agatagcaac caccagaaat 15960
aaaccgccca cgacatccct aattgtagtc actaaaaact ggtagcagta attggttcac 16020
taaaaaatga ccataatcac gaaaactata acagtcacca gaactatata ggtttcatta 16080
gtaattgctt cactaaaaaa tgtccccatg tgtaaatttt catgtcgtat gtatagacct 16140
aaaaacaagg gtatatgact acttgctatg ctaaaacaat agctagcact aaacagtgta 16200
ttcagttaat catccaaaca attttgtgat taatcactaa aacaataccc atggagcaaa 16260
ttatatgaga ataagatctc gtcgttccta ttgtgaagaa tatactacta cctccagttt 16320
caaattacaa tttcaaatta caagttgttt agaacatcca caaggtaatt gcgaagaata 16380
tactaattgc tctagtttca aattacaagt tgtttagaga aaaggtgttc cttaatagtc 16440
tagttttaga agctacatcc aactggtaaa cataaattgc agaaaccttt tatgtggaag 16500
cctccgtcat tgagtctgtc ccctttagct gtaagtagtt ttctaaatat tgttagtcag 16560
gcttagttgt ttgagactct gtttccattc gtgaccatgg gaactgtgaa atgtgtagaa 16620
gatgctcatg ctcatgcata tgcatcgaat tgttttgtaa agtcatctta atgctcaaac 16680
agttttttta tctgccccag ctgtacactg ctttctgaat tatgtcattt aggcttagct 16740
gtccgagata atttcatttg tgatgaaggt aaccggagca tctgtccttt tgttttaaac 16800
ataaatattt tgatagctta acttgtgcgt cattttatca tgtactaaca tggtatatag 16860
atggcactta gcagtaacat tcctgactta tgtgattgtc ctgttagaat gcttctgcaa 16920
tataatgggc ttatgctaat ctgtttggaa tcccatgatg aataacaatt atggatgttg 16980
ggcattttgt atttttatga tgtaggctta acatattttc ttctctgctc agccctgccg 17040
gtggagacct ctatttatag ctataatcca gccatatcta gtgaactaac agatctattc 17100
ctatctctag tgcttatccc cttaacagat ttattttcct atctctattt ctttgatgtt 17160
acttgctgca ggtgctatcc cccactgatg cattatatga caatcactcg aagaatcaga 17220
tgctaattaa ttgttttata cttgaccatt gctaattaag tactccattt ttta 17274
<210> 14
<211> 1767
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 14
atgggttctt tggggcttcc tgtttcattc agcacaagta aagtgaacaa gaacacatgc 60
aacaagggaa agaaaaaagg aagacaagca ccgctcaaag cagcaaacac tcaaatcaat 120
gatgctgtga ggatatgtat caatactgaa gatagagaaa attctgttga atcattggat 180
gctatggagc aaacgcactc atgcaattta tttgtgacac cactgggtca aaatgaaccc 240
tcccgtgatg acactgacaa gaggcttagg gaagacagct cttgtgttga agaacaagaa 300
gagtctggct gtagcaccat ctactctgct ggcaaagccc ctggctgtga tgctaaaaat 360
catctcactg aacttggggc ttttgagctt tctgataact tggccaactc agcaaaagaa 420
gaatactcaa ttcaagaaaa tcaagcttat gaaagtgtgt tgctagattc tgaagagatg 480
tcaaggaatg actgtgttga tgatgaatct acacattcct gtgttggcat ttatcaggat 540
gaaagagtgt ccacaagggg agatcaaaca tctgaagaaa ctctatcagt accccatgat 600
tacaatgatg ttggcagaga agctagtcta agtttggcag agccatcatc tattgatgag 660
catgcacaaa gctctgccaa caacttttac tatgactatg gtgaatggag ggttatctgg 720
gatccattct ataatcggta ttatttttac aacatccaga cacaagagtc cacatggtgt 780
cctcctgaag gactggagga ttttgcatca tattgtagcc cagataccac taaagagcta 840
gctgaactgg gatctcagtg ttcaagcatg gcaccacaag agaacaatct ggctactcat 900
gtcaatcatt tagaagcaca ggagcaagat cactgcattc atgatttatc tgacattcct 960
gttgaaaagc caatatatca aagtatgata actacctctg acaaagcaca gcacactgaa 1020
aataagtaca gcgattcaac aactactgtg ttagagatga accaggaagt tgctagcacc 1080
aaaacgaaaa agagagtaag gagatctcga tcgtatcatt catgccaaga catggcaggg 1140
aacgtctcta atgacatcat caagtactgg gctcagcggt attcactttt ctcacttttt 1200
gatagtggta taaagatgga tgaagtaggg tggttttcag ttacgccaga gccaattgca 1260
aagcatcatg catctcgtgt gggtgcggga gtaatgattg attgtttcac aggagttggt 1320
ggaaatgcca tccaatttgc caaaaagtgc aagcatgtaa ttgcagttga tattgatcca 1380
caaaagattg attgcgcgca tcataatgca tccatttatg gagtaaatga tcacatagat 1440
ttcattgtag gtgattttat acatatagct cctcatctga agggagaaac tgctttcatg 1500
tcgcctcctt ggggtggccc tgactatgcc aaagttgatg tttatgatat gaaaagcatg 1560
cttattcctt gtgatgggta ctctcttttt aaactcggaa ccatgatagc ttcaagagta 1620
gtgatgtttc ttcctcgcaa cattgaccta aaccaattgg cggacatgtc cttgtctgtg 1680
gatcccccgt gggcagttga ggtcgagaag aacttcctca acggaaagct gaaagccata 1740
acagcttact ttgaagaaca ggatcgt 1767
<210> 15
<211> 1767
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 15
atgggttctt tggggcttcc tgtttcattc agcacaagta aagtgaacaa gaacacatgc 60
aacaagggaa agaaaaaagg aagacaagca ccgctcaaag cagcaaacac tcaaatcaat 120
gatgctgtga ggatatgtat caatactgaa gatagagaaa attctgttga atcattggat 180
gctatggagc aaacgcactc atgcaattta tttgtgacac cactgggtca aaatgaaccc 240
tcccgtgatg acactgacaa gaggcttagg gaagacagct cttgtgttga agaacaagaa 300
gagtctggct gtagcaccat ctactctgct ggcaaagccc ctggctgtga tgctaaaaat 360
catctcactg aacttggggc ttttgagctt tctgataact tggccaactc agcaaaagaa 420
gaatactcaa ttcaagaaaa tcaagcttat gaaagtgtgt tgctagattc tgaagagatg 480
tcaaggaatg actgtgttga tgatgaatct acacattcct gtgttggcat ttatcaggat 540
gaaagagtgt ccacaagggg agatcaaaca tctgaagaaa ctctatcagt accccatgat 600
tacaatgatg ttggcagaga agctagtcta agtttggcag agccatcatc tattgatgag 660
catgcacaaa gctctgccaa caacttttac tatgactatg gtgaatggag ggttatctgg 720
gatccattct ataatcggta ttatttttac aacatccaga cacaagagtc cacatggtgt 780
cctcctgaag gactggagga ttttgcatca tattgtagcc cagataccac taaagagcta 840
gctgaactgg gatctcagtg ttcaagcatg gcaccacaag agaacaatct ggctactcat 900
gtcaatcatt tagaagcaca ggagcaagat cactgcattc atgatttatc tgacattcct 960
gttgaaaagc caatatatca aagtatgata actacctctg acaaagcaca gcacactgaa 1020
aataagtaca gcgattcaac aactactgtg ttagagatga accaggaagt tgctagcacc 1080
aaaacgaaaa agagagtaag gagatctcga tcgtatcatt catgccaaga catggcaggg 1140
aacgtctcta atgacatcat caagtactgg gctcagcggt attcactttt ctcacttttt 1200
gatagtggta taaagatgga tgaagtaggg tggttttcag ttacgccaga gccaattgca 1260
aagcatcatg catctcgtgt gggtgcggga gtaatgattg attgtttcac aggagttggt 1320
ggaaatgcca tccaatttgc caaaaagtgc aagcatgtaa ttgcagttga tattgatcca 1380
caaaagattg attgcgcgca tcataatgca tccatttatg gagtaaatga tcacatagat 1440
ttcattgtag gtgattttat acatatagct cctcatctga agggagaaac tgctttcatg 1500
tcgcctcctt ggggtggccc tgactatgcc aaagttgatg tttatgatat gaaaagcatg 1560
cttattcctt gtgatgggta ctctcttttt aaactcggaa ccatgatagc ttcaagagta 1620
gtgatgtttc ttcctcgcaa cattgaccta aaccaattgg cggacatgtc cttgtctgtg 1680
gatcccccgt gggcagttga ggtcgagaag aacttcctca acggaaagct gaaagccata 1740
acagcttact ttgaagaaca ggatcgt 1767
<210> 16
<211> 434
<212> PRT
<213> Zea mays
<400> 16
Met Gly Ser Ser Glu Glu His Val Phe Leu Asp Pro Thr Arg Ile Cys
1 5 10 15
Ala Ser Val Ser Leu Leu Ala His Asp Leu Ile Gly Arg Met Leu Asn
20 25 30
Arg Glu Val Ser Ser Arg Pro Asn Ala Lys Glu Val Leu Pro Pro Met
35 40 45
Ile His Arg Glu Ile Val Arg Phe Gly Tyr Cys Glu Ser Ser Ser Ser
50 55 60
Lys Ser Ser Ser Asp Asn Ser Glu Glu Arg Asp Glu Cys Gly Ile Val
65 70 75 80
Asp Ala Leu Val Thr Thr Ile Thr Gln Ile Arg Lys Met Asp Leu Glu
85 90 95
Ala Arg Ser Leu Gln Pro Ser Ile Lys Ala Gly Leu Leu Ala Lys Leu
100 105 110
Arg Glu Tyr Lys Ser Asp Leu Asn Asn Val Lys Met Gly Leu Ser Ala
115 120 125
Glu Arg Lys Lys Gln Lys Leu Ser Glu Ile Gln Ser Gly Val Glu Glu
130 135 140
Ala Glu Ser Leu Ile Gln Lys Met Asp Leu Glu Ala Arg Ser Leu Gln
145 150 155 160
Pro Ser Ile Lys Ala Gly Leu Leu Ala Lys Pro Arg Asp Tyr Lys Ser
165 170 175
Asp Leu Asn Asn Val Lys Ser Glu Leu Lys Arg Ile Ser Ala Pro Asn
180 185 190
Ala Ser Gly Leu Ile Ser Tyr Lys Lys Leu Leu Phe His Gly Leu Asp
195 200 205
Leu Trp Thr Ala Leu Ser Leu Pro Gln Pro Leu Gly Arg Ala Ala Leu
210 215 220
Trp Pro Pro His Arg Thr Ile His Gln His Leu Gln Cys Gln Gln Leu
225 230 235 240
Thr Gly Val Ala Gly Ser Leu Ala Tyr Leu Ala Pro Glu Val Leu Leu
245 250 255
Gly Asn Tyr Ser Gln Lys Val Asp Val Trp Ala Ala Gly Val Leu Leu
260 265 270
His Val Leu Leu Met Gly Thr Leu Pro Phe Gln Gly Lys Ser Ile Glu
275 280 285
Ala Ile Phe Asp Val Ile Lys Thr Ala Glu Leu Asp Phe His Asn Ser
290 295 300
Gln Trp Ala Ser Val Ser Leu Leu Ala Tyr Asp Leu Ile Gly Arg Met
305 310 315 320
Leu Asn Arg Glu Val Ser Ser Arg Pro Asp Ala Glu Asp Val Leu Arg
325 330 335
His Pro Trp Val Leu Phe Tyr Thr Asp Cys Leu Gln Lys Ala Glu Phe
340 345 350
Ser Asn Leu Trp Asp Thr Asn Lys Thr Ala Ala Pro Met Ile His Arg
355 360 365
Glu Ile Val Arg Phe Gly Tyr Cys Glu Ser Ser Ser Ser Lys Ser Ser
370 375 380
Ser Asp Asn Ser Glu Glu Arg Asp Glu Cys Gly Ile Val Asp Ala Leu
385 390 395 400
Ala Thr Thr Ile Thr Gln Val Arg Ile Ser Glu Pro Lys Arg Ser Arg
405 410 415
Leu Phe Ser Leu Pro Asn Gly Leu Leu Pro Pro Ser Arg Asn Ser Leu
420 425 430
Arg Thr
<210> 17
<211> 143
<212> PRT
<213> Zea mays
<400> 17
Met Leu Asn Arg Glu Val Ser Ser Arg Pro Asn Ala Lys Glu Val Leu
1 5 10 15
Arg Lys Phe Lys His Pro Cys Asn Leu Cys Phe Ile Tyr Met Ile Leu
20 25 30
Asn Leu Ser Leu Thr Phe Pro Asn Gly Phe Gln His Arg Ala Pro Trp
35 40 45
Val Leu Phe Tyr Thr Asp Cys Pro Gln Lys Ala Glu Phe Ser Asn Ile
50 55 60
Trp Asp Thr Asn Lys Thr Ala Ala Pro Met Ile His Arg Glu Ile Val
65 70 75 80
Arg Phe Gly Tyr Cys Glu Ser Ser Ser Ser Lys Ser Ser Ser Asp Asn
85 90 95
Ser Glu Glu Arg Asp Glu Cys Gly Ile Val Asp Ala Leu Val Thr Thr
100 105 110
Ile Thr Gln Val Arg Ile Ser Glu Pro Lys Arg Ser Arg Leu Phe Ser
115 120 125
Leu Pro Asn Gly Leu Leu Pro Pro Ser Arg Asn Ser Leu Arg Thr
130 135 140
<210> 18
<211> 162
<212> PRT
<213> Zea mays
<400> 18
Met Glu Gly Gly Arg His Pro Ser Pro Pro Pro Arg Ile Ser Arg Gln
1 5 10 15
Pro Pro Pro Tyr Pro Ala Cys Pro Ser Ile Leu Pro Pro Leu Pro Pro
20 25 30
Val Asn Val Thr Asn Pro Gly Leu Val Pro Leu Val Val Ala Thr Leu
35 40 45
Phe Asp Glu Arg Val Thr Glu Leu Leu Ser Val Leu Ala Asp Ala Ala
50 55 60
Val Gly Arg Pro Gly Arg Trp Ser Ile Gly Glu Ala Pro Trp Ser Ser
65 70 75 80
Ser Gly Gly Thr Asn Gln Ala Val Tyr Ala Arg Arg Ala Pro Gly Ser
85 90 95
Ser Ser Pro Pro Pro Ala Pro Ala Ser Pro Pro Leu Pro Ser Ser Arg
100 105 110
Ala Asp Cys Leu Ala Arg Trp Pro Gly Ser Arg Ala Leu Val Ala Pro
115 120 125
Leu Gly Thr Pro Ala Phe Val Asp Arg Leu Phe Trp Ser Asp Phe Ser
130 135 140
Gly Ser Ile Arg Arg Glu Glu Glu Ala Glu Ala Leu Arg Asp Pro Ile
145 150 155 160
Arg Arg
<210> 19
<211> 87
<212> PRT
<213> Zea mays
<400> 19
Met Asp Leu Glu Ala Arg Ser Leu Gln Pro Ser Ile Lys Ala Gly Leu
1 5 10 15
Leu Ala Lys Pro Arg Asp Tyr Lys Ser Asp Leu Asn Asn Val Lys Ser
20 25 30
Glu Leu Lys Arg Ile Ser Ala Pro Asn Ala Arg Phe Gly Arg Trp Thr
35 40 45
Trp Lys Gln Gly Ala Tyr Asn Leu Ala Leu Arg Val Ser Ser Arg Gly
50 55 60
Tyr Leu Arg Pro Leu Pro Gly Arg Leu Pro Gly Arg Ser Ser Trp Ser
65 70 75 80
Leu Glu Trp Leu Ile Leu Ser
85
<210> 20
<211> 279
<212> PRT
<213> Zea mays
<400> 20
Met Ala His Phe Asp Glu Leu Glu Asp Lys Thr Thr Asp Tyr Val Asp
1 5 10 15
Leu Ser Val Gln Glu Phe Ala Leu Lys Gln Pro Gln Cys Gly Met Ala
20 25 30
Tyr Asn Tyr Tyr Gly Asn Leu Arg Leu Tyr Val Val Ala Asn Lys Ala
35 40 45
Glu Leu Ala Ser Ser Ile Phe Glu Ile Asp Lys Ala Ser Thr Lys Arg
50 55 60
Ile Gly Ala Arg Phe Cys Arg Cys Leu Pro His Thr Arg Met Glu Gly
65 70 75 80
Gly Arg His Pro Ser Pro Pro Pro Arg Ile Ser Arg Gln Pro Gln Pro
85 90 95
Tyr Pro Ala Cys Pro Ser Ile Leu Pro Gln Pro Pro Pro Glu Arg Lys
100 105 110
Lys Gln Lys Leu Ser Glu Ile Gln Ser Gly Val Glu Glu Ala Glu Ser
115 120 125
Leu Ile Gln Lys Met Asp Leu Glu Ala Arg Ser Leu Gln Pro Ser Ile
130 135 140
Lys Ala Ser Leu Leu Ala Lys Leu Arg Glu Tyr Lys Ser Asp Leu Asn
145 150 155 160
Asn Val Lys Ser Glu Leu Lys Arg Ile Ser Ala Pro Asn Ala Arg Gln
165 170 175
Ala Thr Arg Glu Glu Leu Leu Glu Ser Gly Met Ala Asp Thr Leu Ala
180 185 190
Pro Glu Gln Glu Gln Leu Ala Cys Ala Ala Ala Ala Leu Ala Val Gly
195 200 205
Pro Ala Tyr Glu Arg Leu Gln Glu Ala Arg Asn Pro Ser Glu Gln Gly
210 215 220
Cys Asn His Asp Lys Gln Ile Glu Gln Ala Tyr Asp Asp Ile Leu Asn
225 230 235 240
Ser Ser Lys His Thr Leu Ala Ser Met Met Glu Leu Gln Glu Ala Leu
245 250 255
Leu Glu Ser Asn Gln Ala Thr Lys Asp Ala Asn Gly Ile Ala Ala Leu
260 265 270
Tyr Ile Val Leu Val Leu Met
275
<210> 21
<211> 428
<212> PRT
<213> Zea mays
<400> 21
Met Ala Ser Tyr Ser Ser Arg Arg Pro Cys Asn Thr Cys Ser Thr Lys
1 5 10 15
Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln Arg
20 25 30
Val Thr Val Leu Thr Val Asp Gly Gly Gly Val Arg Gly Leu Ile Pro
35 40 45
Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp Gly
50 55 60
Pro Glu Ala Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr Ser
65 70 75 80
Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp Lys
85 90 95
Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn His Phe Tyr Met Gln Asn
100 105 110
Cys Pro Arg Ile Phe Pro Gln Lys Ser Arg Leu Ala Ala Ala Met Ser
115 120 125
Ala Leu Arg Lys Pro Lys Tyr Asn Gly Lys Cys Met Arg Ser Leu Ile
130 135 140
Arg Ser Ile Leu Gly Glu Thr Arg Val Ser Glu Thr Leu Thr Asn Val
145 150 155 160
Ile Ile Pro Ala Phe Asp Ile Arg Leu Leu Gln Pro Ile Ile Phe Ser
165 170 175
Thr Tyr Asp Ala Lys Ser Thr Pro Leu Lys Asn Ala Leu Leu Ser Asp
180 185 190
Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala His Tyr
195 200 205
Phe Gln Thr Glu Asp Ala Asn Gly Lys Glu Arg Glu Tyr Asn Leu Ile
210 215 220
Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala Met Thr Gln
225 230 235 240
Ile Thr Lys Lys Met Leu Ala Ser Lys Asp Lys Ala Glu Glu Leu Tyr
245 250 255
Pro Val Lys Pro Ser Asn Cys Arg Arg Phe Leu Val Leu Ser Ile Gly
260 265 270
Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg Gln Cys Ser
275 280 285
Arg Trp Gly Ile Cys Arg Trp Leu Arg Asn Asn Gly Met Ala Pro Ile
290 295 300
Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp Ile His Val
305 310 315 320
Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr Leu Arg Ile
325 330 335
Gln Asp Asn Ser Leu Arg Gly Ala Ala Ala Thr Val Asp Ala Ala Thr
340 345 350
Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg Met Leu Ala
355 360 365
Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr Glu Pro Val
370 375 380
Thr Gly Glu Gly Ser Asn Ala Asp Ala Leu Gly Gly Leu Ala Arg Gln
385 390 395 400
Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg Arg Val Ser Ala Ile
405 410 415
Asn Pro Arg Gly Ser Arg Cys Ala Ser Tyr Asp Ile
420 425
<210> 22
<211> 401
<212> PRT
<213> Zea mays
<400> 22
Met Ala Ser Tyr Ser Ser Arg Arg Pro Cys Asn Thr Cys Ser Thr Lys
1 5 10 15
Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln Arg
20 25 30
Val Thr Val Leu Thr Val Asp Gly Gly Gly Val Arg Gly Leu Ile Pro
35 40 45
Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp Gly
50 55 60
Pro Glu Ala Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr Ser
65 70 75 80
Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp Lys
85 90 95
Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn His Phe Tyr Met Gln Asn
100 105 110
Cys Pro Arg Ile Phe Pro Gln Lys Ser Arg Leu Ala Ala Ala Met Ser
115 120 125
Ala Leu Arg Lys Pro Lys Tyr Asn Gly Lys Cys Met Arg Ser Leu Ile
130 135 140
Arg Ser Ile Leu Gly Glu Thr Arg Ala Lys Ser Thr Pro Leu Lys Asn
145 150 155 160
Ala Leu Leu Ser Asp Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr
165 170 175
Leu Pro Ala His Tyr Phe Gln Thr Glu Asp Ala Asn Gly Lys Glu Arg
180 185 190
Glu Tyr Asn Leu Ile Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met
195 200 205
Val Ala Met Thr Gln Ile Thr Lys Lys Met Leu Ala Ser Lys Asp Lys
210 215 220
Ala Glu Glu Leu Tyr Pro Val Lys Pro Ser Asn Cys Arg Arg Phe Leu
225 230 235 240
Val Leu Ser Ile Gly Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr
245 250 255
Ala Arg Gln Cys Ser Arg Trp Gly Ile Cys Arg Trp Leu Arg Asn Asn
260 265 270
Gly Met Ala Pro Ile Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu
275 280 285
Val Asp Ile His Val Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly
290 295 300
Asp Tyr Leu Arg Ile Gln Asp Asn Ser Leu Arg Gly Ala Ala Ala Thr
305 310 315 320
Val Asp Ala Ala Thr Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly
325 330 335
Glu Arg Met Leu Ala Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly
340 345 350
Arg Tyr Glu Pro Val Thr Gly Glu Gly Ser Asn Ala Asp Ala Leu Gly
355 360 365
Gly Leu Ala Arg Gln Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg
370 375 380
Arg Val Ser Ala Ile Asn Pro Arg Gly Ser Arg Cys Ala Ser Tyr Asp
385 390 395 400
Ile
<210> 23
<211> 380
<212> PRT
<213> Zea mays
<400> 23
Met Ala Ser Tyr Ser Ser Arg Arg Pro Cys Asn Thr Cys Ser Thr Lys
1 5 10 15
Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln Arg
20 25 30
Val Thr Val Leu Thr Val Asp Gly Gly Gly Val Arg Gly Leu Ile Pro
35 40 45
Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp Gly
50 55 60
Pro Glu Ala Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Gly Thr Ser
65 70 75 80
Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp Lys
85 90 95
Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn Tyr Phe Tyr Met Glu Asn
100 105 110
Cys Pro Arg Ile Phe Pro Gln Lys Ser Arg Leu Ala Ala Ala Met Ser
115 120 125
Ala Leu Arg Lys Pro Lys Tyr Asn Gly Lys Cys Met Arg Ser Leu Ile
130 135 140
Arg Ser Ile Leu Gly Glu Thr Arg Val Ser Glu Thr Leu Thr Asn Val
145 150 155 160
Ile Ile Pro Ala Phe Asp Ile Arg Leu Leu Gln Pro Ile Ile Phe Ser
165 170 175
Thr Tyr Asp Ala Lys Ser Thr Pro Leu Lys Asn Ala Leu Leu Ser Asp
180 185 190
Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala His Tyr
195 200 205
Phe Gln Thr Glu Asp Ala Asn Gly Lys Glu Arg Glu Tyr Asn Leu Ile
210 215 220
Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala Met Thr Gln
225 230 235 240
Ile Thr Lys Lys Met Leu Ala Ser Lys Asp Lys Ala Glu Glu Leu Tyr
245 250 255
Pro Val Asn Pro Ser Asn Cys Arg Arg Phe Leu Val Leu Ser Ile Gly
260 265 270
Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg Gln Cys Ser
275 280 285
Arg Trp Gly Ile Cys Arg Trp Leu Arg Asn Asn Gly Met Ala Pro Ile
290 295 300
Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp Ile His Val
305 310 315 320
Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr Leu Arg Ile
325 330 335
Gln Asp Asn Ser Leu Arg Gly Ala Ala Ala Thr Val Asp Ala Ala Thr
340 345 350
Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg Met Leu Ala
355 360 365
Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Ser
370 375 380
<210> 24
<211> 589
<212> PRT
<213> Zea mays
<400> 24
Met Gly Ser Leu Gly Leu Pro Val Ser Phe Ser Thr Ser Lys Val Asn
1 5 10 15
Lys Asn Thr Cys Asn Lys Gly Lys Lys Lys Gly Arg Gln Ala Pro Leu
20 25 30
Lys Ala Ala Asn Thr Gln Ile Asn Asp Ala Val Arg Ile Cys Ile Asn
35 40 45
Thr Glu Asp Arg Glu Asn Ser Val Glu Ser Leu Asp Ala Met Glu Gln
50 55 60
Thr His Ser Cys Asn Leu Phe Val Thr Pro Leu Gly Gln Asn Glu Pro
65 70 75 80
Ser Arg Asp Asp Thr Asp Lys Arg Leu Arg Glu Asp Ser Ser Cys Val
85 90 95
Glu Glu Gln Glu Glu Ser Gly Cys Ser Thr Ile Tyr Ser Ala Gly Lys
100 105 110
Ala Pro Gly Cys Asp Ala Lys Asn His Leu Thr Glu Leu Gly Ala Phe
115 120 125
Glu Leu Ser Asp Asn Leu Ala Asn Ser Ala Lys Glu Glu Tyr Ser Ile
130 135 140
Gln Glu Asn Gln Ala Tyr Glu Ser Val Leu Leu Asp Ser Glu Glu Met
145 150 155 160
Ser Arg Asn Asp Cys Val Asp Asp Glu Ser Thr His Ser Cys Val Gly
165 170 175
Ile Tyr Gln Asp Glu Arg Val Ser Thr Arg Gly Asp Gln Thr Ser Glu
180 185 190
Glu Thr Leu Ser Val Pro His Asp Tyr Asn Asp Val Gly Arg Glu Ala
195 200 205
Ser Leu Ser Leu Ala Glu Pro Ser Ser Ile Asp Glu His Ala Gln Ser
210 215 220
Ser Ala Asn Asn Phe Tyr Tyr Asp Tyr Gly Glu Trp Arg Val Ile Trp
225 230 235 240
Asp Pro Phe Tyr Asn Arg Tyr Tyr Phe Tyr Asn Ile Gln Thr Gln Glu
245 250 255
Ser Thr Trp Cys Pro Pro Glu Gly Leu Glu Asp Phe Ala Ser Tyr Cys
260 265 270
Ser Pro Asp Thr Thr Lys Glu Leu Ala Glu Leu Gly Ser Gln Cys Ser
275 280 285
Ser Met Ala Pro Gln Glu Asn Asn Leu Ala Thr His Val Asn His Leu
290 295 300
Glu Ala Gln Glu Gln Asp His Cys Ile His Asp Leu Ser Asp Ile Pro
305 310 315 320
Val Glu Lys Pro Ile Tyr Gln Ser Met Ile Thr Thr Ser Asp Lys Ala
325 330 335
Gln His Thr Glu Asn Lys Tyr Ser Asp Ser Thr Thr Thr Val Leu Glu
340 345 350
Met Asn Gln Glu Val Ala Ser Thr Lys Thr Lys Lys Arg Val Arg Arg
355 360 365
Ser Arg Ser Tyr His Ser Cys Gln Asp Met Ala Gly Asn Val Ser Asn
370 375 380
Asp Ile Ile Lys Tyr Trp Ala Gln Arg Tyr Ser Leu Phe Ser Leu Phe
385 390 395 400
Asp Ser Gly Ile Lys Met Asp Glu Val Gly Trp Phe Ser Val Thr Pro
405 410 415
Glu Pro Ile Ala Lys His His Ala Ser Arg Val Gly Ala Gly Val Met
420 425 430
Ile Asp Cys Phe Thr Gly Val Gly Gly Asn Ala Ile Gln Phe Ala Lys
435 440 445
Lys Cys Lys His Val Ile Ala Val Asp Ile Asp Pro Gln Lys Ile Asp
450 455 460
Cys Ala His His Asn Ala Ser Ile Tyr Gly Val Asn Asp His Ile Asp
465 470 475 480
Phe Ile Val Gly Asp Phe Ile His Ile Ala Pro His Leu Lys Gly Glu
485 490 495
Thr Ala Phe Met Ser Pro Pro Trp Gly Gly Pro Asp Tyr Ala Lys Val
500 505 510
Asp Val Tyr Asp Met Lys Ser Met Leu Ile Pro Cys Asp Gly Tyr Ser
515 520 525
Leu Phe Lys Leu Gly Thr Met Ile Ala Ser Arg Val Val Met Phe Leu
530 535 540
Pro Arg Asn Ile Asp Leu Asn Gln Leu Ala Asp Met Ser Leu Ser Val
545 550 555 560
Asp Pro Pro Trp Ala Val Glu Val Glu Lys Asn Phe Leu Asn Gly Lys
565 570 575
Leu Lys Ala Ile Thr Ala Tyr Phe Glu Glu Gln Asp Arg
580 585
<210> 25
<211> 589
<212> PRT
<213> Zea mays
<400> 25
Met Gly Ser Leu Gly Leu Pro Val Ser Phe Ser Thr Ser Lys Val Asn
1 5 10 15
Lys Asn Thr Cys Asn Lys Gly Lys Lys Lys Gly Arg Gln Ala Pro Leu
20 25 30
Lys Ala Ala Asn Thr Gln Ile Asn Asp Ala Val Arg Ile Cys Ile Asn
35 40 45
Thr Glu Asp Arg Glu Asn Ser Val Glu Ser Leu Asp Ala Met Glu Gln
50 55 60
Thr His Ser Cys Asn Leu Phe Val Thr Pro Leu Gly Gln Asn Glu Pro
65 70 75 80
Ser Arg Asp Asp Thr Asp Lys Arg Leu Arg Glu Asp Ser Ser Cys Val
85 90 95
Glu Glu Gln Glu Glu Ser Gly Cys Ser Thr Ile Tyr Ser Ala Gly Lys
100 105 110
Ala Pro Gly Cys Asp Ala Lys Asn His Leu Thr Glu Leu Gly Ala Phe
115 120 125
Glu Leu Ser Asp Asn Leu Ala Asn Ser Ala Lys Glu Glu Tyr Ser Ile
130 135 140
Gln Glu Asn Gln Ala Tyr Glu Ser Val Leu Leu Asp Ser Glu Glu Met
145 150 155 160
Ser Arg Asn Asp Cys Val Asp Asp Glu Ser Thr His Ser Cys Val Gly
165 170 175
Ile Tyr Gln Asp Glu Arg Val Ser Thr Arg Gly Asp Gln Thr Ser Glu
180 185 190
Glu Thr Leu Ser Val Pro His Asp Tyr Asn Asp Val Gly Arg Glu Ala
195 200 205
Ser Leu Ser Leu Ala Glu Pro Ser Ser Ile Asp Glu His Ala Gln Ser
210 215 220
Ser Ala Asn Asn Phe Tyr Tyr Asp Tyr Gly Glu Trp Arg Val Ile Trp
225 230 235 240
Asp Pro Phe Tyr Asn Arg Tyr Tyr Phe Tyr Asn Ile Gln Thr Gln Glu
245 250 255
Ser Thr Trp Cys Pro Pro Glu Gly Leu Glu Asp Phe Ala Ser Tyr Cys
260 265 270
Ser Pro Asp Thr Thr Lys Glu Leu Ala Glu Leu Gly Ser Gln Cys Ser
275 280 285
Ser Met Ala Pro Gln Glu Asn Asn Leu Ala Thr His Val Asn His Leu
290 295 300
Glu Ala Gln Glu Gln Asp His Cys Ile His Asp Leu Ser Asp Ile Pro
305 310 315 320
Val Glu Lys Pro Ile Tyr Gln Ser Met Ile Thr Thr Ser Asp Lys Ala
325 330 335
Gln His Thr Glu Asn Lys Tyr Ser Asp Ser Thr Thr Thr Val Leu Glu
340 345 350
Met Asn Gln Glu Val Ala Ser Thr Lys Thr Lys Lys Arg Val Arg Arg
355 360 365
Ser Arg Ser Tyr His Ser Cys Gln Asp Met Ala Gly Asn Val Ser Asn
370 375 380
Asp Ile Ile Lys Tyr Trp Ala Gln Arg Tyr Ser Leu Phe Ser Leu Phe
385 390 395 400
Asp Ser Gly Ile Lys Met Asp Glu Val Gly Trp Phe Ser Val Thr Pro
405 410 415
Glu Pro Ile Ala Lys His His Ala Ser Arg Val Gly Ala Gly Val Met
420 425 430
Ile Asp Cys Phe Thr Gly Val Gly Gly Asn Ala Ile Gln Phe Ala Lys
435 440 445
Lys Cys Lys His Val Ile Ala Val Asp Ile Asp Pro Gln Lys Ile Asp
450 455 460
Cys Ala His His Asn Ala Ser Ile Tyr Gly Val Asn Asp His Ile Asp
465 470 475 480
Phe Ile Val Gly Asp Phe Ile His Ile Ala Pro His Leu Lys Gly Glu
485 490 495
Thr Ala Phe Met Ser Pro Pro Trp Gly Gly Pro Asp Tyr Ala Lys Val
500 505 510
Asp Val Tyr Asp Met Lys Ser Met Leu Ile Pro Cys Asp Gly Tyr Ser
515 520 525
Leu Phe Lys Leu Gly Thr Met Ile Ala Ser Arg Val Val Met Phe Leu
530 535 540
Pro Arg Asn Ile Asp Leu Asn Gln Leu Ala Asp Met Ser Leu Ser Val
545 550 555 560
Asp Pro Pro Trp Ala Val Glu Val Glu Lys Asn Phe Leu Asn Gly Lys
565 570 575
Leu Lys Ala Ile Thr Ala Tyr Phe Glu Glu Gln Asp Arg
580 585
<210> 26
<211> 13229
<212> DNA
<213> Zea mays
<220>
<221> misc_feature
<222> (10689)..(10788)
<223> n is a, c, g, or t
<400> 26
ccccgcgcgg ccaaccctct ctaagagggc cctggtcctt ccttttatag tcgtaaggag 60
tggatccagg tgtacaacgg gggtgtagca gagtgctacg tgtctagcgg gggagagcta 120
gcgccctaag tacatgccga tgtggcagcc ggagagatct tggcacccag cgagtgtgat 180
gtcgtggcca tcggaggagc gacggagcct ggcggaggga cagctgttgg agcggttgag 240
tccttgctga cgtcctcctg cttccgtaag agagctgaga gccgccgtcg tcacagagct 300
tgcggggcgc catcattgcc tatctggcgg agctagccag ataggacacc ggtcttgttc 360
tctgcggccc gagtcggctc ggggcagggt gatgatggcg cttcctgttg acgtgactgg 420
cctgcgccct aggtcgggcg acgtggaggc tcctccgaag ccgaggtcga gtctgtcttc 480
catggtcgag gccgagcccg agcccctggg tcgggcgagg cggaggtcgt tcggcagagg 540
ccagggcgga gtccgagccc tggggtcggg cgaagcggag ttcgtcgtct tctggggctg 600
agcccgagtc cgagccctgg gtcgggcgga gcggagttcg ccgtcttccg ggactttagc 660
ccgagtccga gccctgggtc gggcggagcg gagttcgccg tcttccgggg cttagcccga 720
gtccgagccc tgggtcgggc ggagcggagt tcgccgtctt ccgggactta ccccgagtcc 780
gagccctggg gtcgggcgga gcttcctatg gtgcctttgg cagggcctga ctgcccgtca 840
gtctcactct gtcgagtggc actgcagtcg gagtggcgca ggcggcgctg tccttctgcc 900
aggccggtca gtggagcggc gaagtgacgg cggtcacttc ggctctgccg gagggcgtgt 960
gtcaggataa aggtgtcagg ccacctttgc gttaaatgct cctgcgattc ggtcggtcgg 1020
tgcggcgatt tagtcagggt tgcttcttag cgaaggcaag gcctcgggcg agccggagat 1080
gtgtccgccg ttggaggggg gcctcgggcg agacggaaat cctccggggt cggctgccct 1140
tgtccgaggc taggctcggg cgaggcgtga tcgagtcgct cgaatggact gatccttgac 1200
ttaatcgcac ccatcgggcc tttgcagctt tatgctgatg ggggttacca gctgagaatt 1260
aggcgtcttg agggtacccc taattatggt ccccgacaac cacaaacgcc cacgtcgtgc 1320
gcgtggaggt aaggctatct gcattcatca tactttaaac tagttgggtg cccgtgcgtt 1380
gcaacagata tcatataaat tcatgtgttt tgctacgcga cacgagggat cggtattatt 1440
agtagtcatt tttctattgg acctagtgag gtactcgtac gttgctacgg agatcatata 1500
aatccatgtg ttttgctacg cgacacgagg gatcggtatt attagaagtc attttctatt 1560
ggacctagtg aggtacctgt atgtgcccgt acgttaccac gagagttaaa acctagtata 1620
aaacataaat acagaatgac aaacatcatt atgatataca aattcgtgta acaaaatatc 1680
actgtagcac taatattcaa taaaatgtag caactcactg ctcactaatg atgtgtccag 1740
ggtaagtggg taaagcacgg tagacgtctt cttttgactt agtacaatgc tcgtgtgttg 1800
agacggtgca caattattcg ataaaatgtt tgagcacaag tgcacaacga ttacataaaa 1860
ttgaaaaata cttatattaa caaagtctaa ttgtctcaaa ttctttttgc caacaaccaa 1920
ttcactgtgt tgcgatgata cacaataata tttcatgaat gactcaggca atccacctaa 1980
gtgggtaacc caaaagcaca ccaatgtgat gcctacacgt gaacatctaa cctctattac 2040
tacaatagtc ttgttggaaa tttacaactc atctagaatt tcaaaatact caaaattatt 2100
tagactctct ctaaaccgga gcatacaaaa tagatcaggc agttttaacg gaaacatgta 2160
tgactctcac catcctgtgc agcgctgctc gaataccaga aagtcccagc tcagccaata 2220
ctgcatcaag ttctgctagt tccttttttt catctccttt ttagacaact gtctttctgt 2280
ctctttgggt ggcgcagcta gggcccacaa ctgtcttgat agcaggttcg gctggggcat 2340
ctttcggatc atgctttagg ttcatcctca gctccatcat caacctcaaa atcaagatcc 2400
ttgtcatcac tctcaatgtc ctgcaacata tatagaagta catctaagaa cactatcaga 2460
gaggctaact ataatgacaa tcagccaaac atgctccatc cccctcgaga tgagtaaatt 2520
atacttccac ttaatagttt agttttgatg caaaaattat gacaaaaaac aagtagaaca 2580
ataaccagag tttgctatga caaacatgca gcacctgtca tgtataacca tttccaaatc 2640
acttgcttac ccaagatata gaaaaacttg gaacaagtcc ctatccaagg tacatatttt 2700
ttatttttgt ttagcaggtg gtcagctctg aaaattttct gtcaaggtga agtttgcctt 2760
atgacttgct catagcaggg tctgctttga actcctccac tgggaatgca atgtcgtgat 2820
cccacagacc gatcaaatgg gattcaaatt taggctgtgc tatatcgcgc tctgaatcta 2880
gtgataatgc gtctatcctt ggtttcttgg gagaatgttg catgatctct gggtagtcat 2940
ctaagcttgg aggtgaatca gaaagtttct gaccctcaga agctttaatt gatccaccaa 3000
gcttttgttg ctcctcaatg atcatctgca agtatattcc ttgtgcttta attgttagtt 3060
gcagttgtct ttgaacctga aaacataact tatttagaca agcaaaagaa agttggcaag 3120
gaaaatttag attgatcttg aagctaaaaa ggactcataa actacaagca ataagacaaa 3180
acaggagaac tgccaacaat agattattga caactaagca ttactacaac catgagaaga 3240
aaccactgtt aggtgtaacg tgacagttaa taagtgaata ttgagaatgg gccaaaatat 3300
cagcttgtag aagtagcccc aactaagtag gacttctggt catagtagga ttgtcggtaa 3360
actaaaatga tgtaaaaaac agaaatctct actacttatt aagtaagcaa tagtagtctg 3420
cctccctcgt tctgccgttc tgccatccat cgatctggac cgttcatatc gcatcgtgcg 3480
tctgcgtctc ccacactcta tctccctcca ccgcacgacc acccaatccc taaccttccc 3540
ccacacgttc ctctctctct cgcgttgccc gccccctgcc cctgccgcga tagaatgcct 3600
cgcggcctcc accgccgacg cggcctccac cgcttccctt cctccctttg cctccaccgc 3660
ccctccccca acgtcgtcgc caccgccgct ctagggcctc gacgcaggca gcttgggaac 3720
cgccctcggc ctcggtgtcg cacggcccca cgaggaccga ccttgccgca tggcggttgt 3780
ggaggcgtgc gaccccgcaa ggatcgacat ctcgtccaag acagcagccc cctcagagcg 3840
gacgacgcag cttatctctg gtggaaggca cgtagccacg cccccactcc ctcctcgtgc 3900
tcccgccgac gcctgcctcg cgctcctcgt cgaccgtctc tccctctcct ccgtcgttgc 3960
atctcgcctc tcgcgcacgc acaaaggtga gtctcccctc ccccgactca cccctctagg 4020
tctctgattc ttctcatcgg cgagcaagga cggaggagct ggcggaggag ctgtcgaata 4080
taacctaatt atgtcggtct cgggtgggat ttggctattt tgggcgccat ggcctaggtg 4140
agcgtacaga tctggttctg cattttgttt tcttatctgt caatcatgtt ttttcatatt 4200
actgcaggtg ggtctgatta aatattacag acatgttttg cttcgaagcc ctacacctgt 4260
ccatctaatt ttaggaactg acgattgaca cagctttgtt gtggcgctat ttagtataag 4320
ttatagaaat cggatctttg gctctcatct gcttgtagtt gcaacattga ggtagaacct 4380
gggtgggtct gattgctcat tgctgtgata tatgtctgga aaagcagagt tatagtttgg 4440
aaactagcgc gtagtacatg tcggtttctt taagtaattg cttatgctct gttgttttga 4500
tttccagatg ccccaaatgt gctgaactta agctatcgcg tgagaacaca actttctggt 4560
aagtggcaag ctcctccgtt tcttatttta ccgcattgga agttggaaca ccatgatagc 4620
tctcaggacg aatgagtatc agctaatttt attgttttaa caatagagca taaaacctta 4680
tgcatatttt aaaggttcat gtatcattta gttatggcct tatgaggaag cttcatcatc 4740
atatctgtag ataccttgat tcgtggggat ggtaattttt tgtattcttg ttcttttgat 4800
ttttgaagga aacacatctt ataatacgat agcacccatt ggaagtcctc gcatacagtg 4860
cagggtagaa ttatgcttca tgtgttgccc cttcaccacg atatgccaaa ttgaatgtag 4920
tttcatcagt tatgctcaat tatggatgtt cagaggcatc caatttcagt gtgtactgca 4980
atacttgggt ccacctatag ttgatagatg ttctgtattt tgttttctta taaattagat 5040
ttggcttgca ttatattgtt ctcttttgga acagagtcac tctccgacag ctcatcgccg 5100
cctgcttcga ccgcctgtac acaaggttta agaagcgctc tgcgaacttc cacggccgcg 5160
ccgtgctgct tctagctatt tacctttttc tatgatgcaa atgtttatat gcatactatg 5220
ttacttgaga aacattaaag tacttgatgt acctaaacac attttgttta gtgatgtata 5280
gtgatatagc attattgtct atattaatat ttatattgct ggtagcttct actttaattg 5340
atctcaatgg ggcatttggg tggctagcaa ttcacattga taatttaaaa gtgaatttca 5400
ggtgtacatt tgatggcctc cgatatggtg ctgccttcaa ttctctacaa tgcgcgagaa 5460
tgctgctcag gagggtatta atggctcaac acagatgacc tcctcggagt catgtttcta 5520
attatctaca ctatgattct ccttctgttg ataaaatatt gttttattgt gctgtgagct 5580
aatgataaca gtgatggtaa gtaaatatgg tccatgcata ttctcatcat agatggctga 5640
aaaactccga gtgctgctac gctaccagag tcttcatgtg catacttact tcaagaactc 5700
aaggtacata gttttctcaa cagaagaata tgtatctgtt tgattccagc tgaattgctt 5760
actaaactca gtgtgtcact ttaaatgata tgggatgaag ttgggcaaga ccaaagtgaa 5820
agtgggagaa tacccgaaga acttcttgtt ggacgaactt ggagaaacca atactaaaac 5880
tcagtgtcaa ccgcttgcaa caggcaattt aggtcgatgt gctcgcacgc tgtgtgacca 5940
tgtctgagca ctccccaccc acccaatcgc ttcccacgtc atgccgccac gtcgagaatt 6000
tgtacacaac tcaggttgcc cattttactc tgttattgaa cctcgctttt ctgtagaccc 6060
aggtatgcta gaagtaggta atggtagcat gaccttagca gagtatcgct cacattttag 6120
catttgggtt gtcatgaagg taagttttca ctgagattgg accgatgttg tgtcatcata 6180
ttttgagtaa agggtttgac cgaagaattt tgaaaaataa aagactgtag ttcattgaag 6240
gtgataactg gtatgcaagt attactatta atgattctcc acttactgat attacatttg 6300
gataagagga aggagatatc ctaattctaa tgggttaact gctagcagtt ccttatgtac 6360
tgttatgctt caggcccctt tattaattgc ctcatgtgga aagagggtgt gatgatcttt 6420
tgttgttttt gtagcgagaa gctcaaagaa ggggaagaca tctacatcta tcagtaagtg 6480
atccagttta gtagatgaat caccgctatg ggtgtttttc tattcttgaa taggcatgtg 6540
gttctattga tggttgtttg atgtttggta tattgtgttc tgactgtagg ggtgacaaat 6600
ccttttgcag catggagtgc agagaaaatt tcatggtaga cgagatggaa ggtgagccag 6660
agagtatgta taatcagatc aattttagac tttttttcac taaccactta tccatagcac 6720
acttgtgttt atttgagata tgtgtcgtca ttttgtgttc atcggctatt gctatgaaga 6780
tgaccacagg gttaattctt gtttatgagt tcatcctcta gaatgtattg ggttgtcgcc 6840
aagaaaaaac acacgaagac ataaattcaa ataatttctg gtgctgtgga gtattcataa 6900
ttgtgtgctt ttgttgaatc cttcatgtac cataattctt actgcttgca agccctttta 6960
gaatgtgact ttaggatgta gaatttggtg aaaggagaca agaaattatt ctctaaacta 7020
ctatttttat aggcaaggga ggctggaagc accttttttg catgggagct gcatggagag 7080
aacttattgg tagaaaacaa actttgacca gattcttgaa tgtgccaagg gaaaaaggta 7140
ttaattggga ccttctattt atacaaggga aaaaggtatt ttttatacaa ttgctcttcc 7200
ttctcatgta tggcaacttc ctgttttgta gagattgagc gcccttcctc actgtttgaa 7260
ctttactcat cctcatgaaa ctgtgatgct acctgtagga gcggtttgtt caaggaggga 7320
atgactttta gaccctaata acttgtctac acttaagata aaaattgttg tcattgctat 7380
ggttacctcc tttagtgtcg taaactcaga ataatcatag atccctttgt tgcttacaca 7440
ttattcatgt tctacaagct attgagattt tgactagccg ctacatttta gtgcaggttt 7500
tcataatttc ttacctttat atctacattt tagatttcct cccttttgac actacatttt 7560
agccgctcac tcggaagctt tccatccctg ctggagtact cagcccataa aggtaaatgc 7620
atgcctttac ctgcctgaaa tgcattgtct ttcttttcaa ccgtgaatgt gaattggatc 7680
catcattcat gtgcacacac aaggccggtt tgaatttgga atgcactgtt gttctgcctg 7740
tgatccactt ggtggttttt atttgcgttc acatattaaa aaatatatta taccctgatt 7800
ctgagtcaaa ctttggttag acctggattt gttgattagt ttccgttgtt gtgatctgtt 7860
gaacaaacta taggttaggt gagttatcag attcttagat ctgtcctgta cggttgtgat 7920
ctgttgaaca aactataggt taggtgagtt atcagattct tagatctgtc ttgtacgtct 7980
gatctctacc atgttacaaa catctgaaag ttaataataa tcactactac attcacacct 8040
atcttgtatg gatttgctgt tgaaattgca gaatgcttcc caacttgtgc atttttatta 8100
catccccaca acttcatcgt atagtccaga catcctttgt ttgtgtagca aaatagatgt 8160
gcaattgttt cattgtaaca atgtcctgta tattaacctc ggcggctctg gtgctttctg 8220
caggcttctc attcttattt gcatggcctg taactgctgc acagcgcgct acggctggcg 8280
tagcggcagc aactgctggt atgaacagga tccgtccacc ctcattagcg acgatgaagt 8340
gacacccaat tctaggcgcg gacgtacgat ggcgccctcg cgcgggatat cgggctgagt 8400
gatgagacgc tgctgccatt ggggttccag gcgcgcctga ccgctcccac tcctaggccc 8460
ggcatgtctc tccgacgcta cctaccggtg ctggtgaaac gcaaacttaa aatcgtgcat 8520
tgcaagatgt tgccttctct ttatgtctcc tctactctac gcctacgagc tttctttgtt 8580
tataactttt tctgtggttt cgctttcaac aagctcaagc ggtcaacaga tcaacttaga 8640
caaaatgcag atgcagtgga aatattaagg aagaccagat tccctcatgt tcatggagca 8700
ggtgaaaaga agtcaccaga gacaattctg gatcatgaga ctatgagagg actttggcaa 8760
caaatgttcg gtttggactt ctctgggtgg ttctatgata ctttataatt tcttttggct 8820
tatgtgtcat ttcagaagat atgaaaatag ctaagcattg tcaataatat tagcttcctt 8880
gtttcttgtt aacctaagat gactgcccta tttcacttgt tttttctagc tgtggtagta 8940
ggtcattaga gtagttccct tcaatcgtag cactcagcca tgttgtaaat ggtttacttc 9000
tattgtgaat cgtgtttttc ttttttaatg gtggggttag tggaaaatct ggacgtatcc 9060
gcgaatggtg gagaagcaca caaaacaaca attatatgca aaaactaaac tatgttttct 9120
ttctttctat ggtatatgaa tttagtacct tgataatcac aatgattaga gtctgtttgt 9180
atttatatta aaacataact gcttaggagg tgtatgagga ggataacgag atgtgcagtg 9240
cgcgcaacag taagcagact accatgactt ggttgctctt caagtgtgca agtgtgtcta 9300
tggattgtat ggttctttgg tttttgttgt tcgctaaggt gcaaagtagg aagaaaatac 9360
ggccctgacc ctgcaagtgg ggaatgtatc gtttttgctg gaactgaaat atgcggtgtt 9420
cttttttatg cactaatatt ttgttgaata ttttgtatac cgttgcaacg cacgggcatc 9480
tacctagtaa attaatagtt ttcaataaaa gacctcgagt ttctcatgta gtcgcttctg 9540
aacctccatt tacatcttta gtgccttaaa aatcgtgcca atgtttccat atatcaaatc 9600
acggtgatca acaattaaac aaaataactc ttaaccaaaa aaatggaagt gtcacgcact 9660
catgaactac agatgtcaat agctaacata taaacaggct caagccttcc ttcaaggata 9720
ccaggttgtt ctaagcagca tctctatatt tagagaggaa gacttgtctc gattcatctc 9780
ttcctgagac cccactcatg tgggtagttc tacctttttt atgaatacag atgcaacatc 9840
aacatataaa caggctcatg cctcagatac tactctcaat tggtctactg aaattctatt 9900
gcatttatga cgaatgcagt tacagattta aaagtaaagg acgagtacag ctgcatgatg 9960
aaaagtaaac agaacggtaa ctgcaaattt acttcagaca tgctgctgct ttgatgagat 10020
cggaacaggg ttgttgctca caatctgtgg atttaaagcc tgcaacacat gtcacaattt 10080
aatcatgagg ttcactaaga caacattaca caaaagaggt gttggtactg agaacataaa 10140
agatcatgtc atccttgtct gaaaggaagt gcattccaac taagaacaaa gatcactgct 10200
tctcaatgaa gcaaggctac gacatgatgc atatttagct caaattaccc aagcaagtgt 10260
ttattaaaat aaaagcatgc atcttagaca tcagcaatag gttatctaag acgtcaatgc 10320
atatttagcc cagttttaga caacaatgca tatttagcta aaatattaaa catgcaatgc 10380
ctctttagct caaatttcag accacagtat taaccggtgg tgctacactt gaagaacaag 10440
cttatctgga gaagcagccc acatccagtc ctgaaggcaa atcagatgtt tgaacaaaat 10500
cagtgcttag ccaaatctag aaaccacaac cactcgaacg ctgactcaga tggtagagcc 10560
atcacaccaa tggtgctacc atcagtaaaa taatagaatc aatttgtttt tccttactga 10620
tacagtaccc ttcgaataat tgtttataag caattgaact tgctgcccaa tgtattatag 10680
tcttggtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10740
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt agagacatat 10800
agagtagtat agataaggat aatggacagt agtacttgaa gttaatacat aaaacttgca 10860
gctaataaac aaacatagta ctattttagt taacatacaa caattttcca gctcttcttt 10920
gcacttatag catttttagt acctacacac aacctgaact gaacaccatc aagaacagta 10980
ctgcatttgg aagtttgaat aaaaatggtg tccaattaac agttttctaa tattgtaact 11040
taattattga atggtgcaat agggtgttca cttgttgaat tggttgataa tcttgtgtgg 11100
gctttaaata taacattgtt agtgaatgct aaataattct gcactcttgg aatggtcaag 11160
ctgagaatga gccagagatt aactggtgcg aacctgggaa gcctgcaggg atggaggtcc 11220
ttcttgggag taaggatggg ttcttgacga agaagattaa gagctatagc acatgccgaa 11280
gtgatcctac aaagccacgt gcattgcaat gcctttcacc actgatagca ggtcatgtaa 11340
gatacatcat ttaaccatct ggatatgtgt taatgttaga caactaattt ataaaatttt 11400
cgattcaggt gggcaatgga attgtttgtt gaatgagagt aatgtttatt attggtaaag 11460
tatcacaatt cagtctgatt caggtgggca tggtaaaatg aagtgcatct gccaaaaagt 11520
aaggtaagta actaagtatc ttttggatgt ttggaggatc agatgttgtt gcagaatgtt 11580
tattgactaa ttgcaagtca aatgaattaa aggggaaaac agataagcac taagattttt 11640
atatgaaagc tgtgtgagtc aacctgctaa gagctctaaa tgtcctgtcc cagaagtagt 11700
ggcggagcca gcccaaattt gaagcctatc gactcagaaa attcatccat gccaaccaaa 11760
caaatagaat aattacctta tctgttgcac gggtgtgaag gagctggaga tgcagatcag 11820
gtgtcacaaa ttctataacc aaattgcata atgcataagc aatcaaacaa tacaccccta 11880
attctttagg ctaaacgaac tgcactaagc ctatgtttca gatttcaaga ttactattgg 11940
attgtgttca tcacaccaat aggttaagta ccaacatgct aatcaataag aacagtaaag 12000
gggcattacc tacaacaaat gttacgtcta aatagaacta acttggaggc aatgtaagct 12060
ctgaatgtcc gaaacattga aacaaagtct gaactctgaa attgtctccc taatcctcac 12120
ctcatcccat ctgtgtcgat ggatggtggg tgcctcgcct gagctgttgt gtggtgccgc 12180
ggacgaggtg agcgcttgtg tagaatgaag atggggtcgc agagcgacgt ggccgacggc 12240
ggaccgatga tggagagggt actttttact ttcattaaca gatcatcaag caaaataaaa 12300
tagtaatgag caattgagcc cagatcacat ttgtaatcat ctagcatgta agcactattt 12360
tttaatttga tacccctcat tcagagtttt tgcacaaact tagaactgga ggctgaaagt 12420
aagcaaattg acttcatctt ttagtttgat gaagatcaaa tgaatttaaa agcttgagaa 12480
gatgaaaaga tctcatatgt atgaccaaga gatgatagca aatggcttca agccaataaa 12540
gtcacgagag atgaaagcaa ataggttcac atgcaggata caacagaaag ttatgtatac 12600
aggaacaaat gctaatagaa ctatgcattt atcatatttt agaaactgta gcccttgttc 12660
atcctacccc caatgagtat tcctatcagt acacatgtag gctagcattt ttttagttgt 12720
aatacttgct tagctacaga tcactggcta gctgaaaaaa acctaagtag caacagtgga 12780
acagtttgag tatagctgta tgaaaaaggc tatctgaata atagcaatat ttggattcat 12840
gttcatttca agaacttcac cattgggaaa actccaacgg tactaccatt tgattgctgc 12900
aatgtttgta atgataaaga gatctcactt actcacttgt ggtaccatag aaacctgcaa 12960
aaaagaggaa aataatataa attggtgtaa aaattaagga attcatagta caaatatgat 13020
cgactttata ggaccaccct tttgttaaat ttttcagtgt caacaggaag gtgtgattca 13080
ggcctttgtg gatcaagtca gcacagaacc ttggcttctt cattcggtag aatgatgaca 13140
agactaccga atgatgacaa gacttttaaa aatatcctgt tgaaaatgca tttaccatgt 13200
cagctgtgtt accaagacgg gtaattact 13229
<210> 27
<211> 1515
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 27
atggcggttg tggaggcgtg cgaccccgca aggatcgaca tctcgtccaa gacagcagcc 60
ccctcagagc ggacgacgca gcttatctct ggtggaaggc acgtagccac gcccccactc 120
cctcctcgtg ctcccgccga cgcctgcctc gcgctcctcg tcgaccgtct ctccctctcc 180
tccgtcgttg catctcgcct ctcgcgcacg cacaaagatg ccccaaatgt gctgaactta 240
agctatcgcg tgagaacaca actttctgct tctactttaa ttgatctcaa tggggcattt 300
gggtggctag caattcacat tgataattta aaagtgaatt tcaggtgtac atttgatggc 360
ctccgatatg gtgctgcctt caattctcta caatgcgcga gaatgctgct caggaggatg 420
gctgaaaaac tccgagtgct gctacgctac cagagtcttc atgtgcatac ttacttcaag 480
aactcaagcg agaagctcaa agaaggggaa gacatctaca tctatcaggg tgacaaatcc 540
ttttgcagca tggagtgcag agaaaatttc atggtagacg agatggaagg caagggaggc 600
tggaagcacc ttttttgcat gggagctgca tggagagaac ttattggtag aaaacaaact 660
ttgaccagat tcttgaatgt gccaagggaa aaaggtatta attgggacct tctatttata 720
caagggaaaa aggcgcggac gtacgatggc gccctcgcgc gggatatcgg gctgagtgat 780
gagacgctgc tgccattggg gttccaggcg cgcctgaccg ctcccactcc taggcccggc 840
atgtctctcc gacgctacct accggtgctg gtgaaacgca aacttaaaat cgtgcattgc 900
aagatgttgc cttctcttta tgtctcctct actctacgcc tacgagcttt ctttgtttat 960
aactttttct gtggtttcgc tttcaacaag ctcaagcggt caacagatca acttagacaa 1020
aatgcagatg cagtggaaat attaaggaag accagattcc ctcatgttca tggagcaggt 1080
gaaaagaagt caccagagac aattctggat catgagacta tgagaggact ttggcaacaa 1140
atgttcggtt tggacttctc tgggtggttc tatgatactt tataatttct tttggcttat 1200
gtgtcatttc agaagatatg aaaatagcta agcattgtca ataatattag cttccttgtt 1260
tcttgttaac ctaagatgac tgccctattt cacttgtttt ttctagctgt ggtagtaggt 1320
cattagagta gttcccttca atcgtagcac tcagccatgt tgtaaatggt ttacttctat 1380
tgtgaatcgt gtttttcttt tttaatggtg gggttagtgg aaaatctgga cgtatccgcg 1440
aatggtggag aagcacacaa aacaacaatt atatgcaaaa actaaactat gttttctttc 1500
tttctatggt atatg 1515
<210> 28
<211> 2229
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 28
attgatctca atggggcatt tgggtggcta gcaattcaca ttgataattt aaaagtgaat 60
ttcaggtgta catttgatgg cctccgatat ggtgctgcct tcaattctct acaatgcgcg 120
agaatgctgc tcaggagggt attaatggct caacacagat gacctcctcg gagtcatgtt 180
tctaattatc tacactatga ttctccttct gttgataaaa tattgtttta ttgtgctgtg 240
agctaatgat aacagtgatg gtaagtaaat atggtccatg catattctca tcatagatgg 300
ctgaaaaact ccgagtgctg ctacgctacc agagtcttca tgtgcatact tacttcaaga 360
actcaagttg ggcaagacca aagtgaaagt gggagaatac ccgaagaact tcttgttgga 420
cgaacttgga gaaaccaata ctaaaactca gtgtcaaccg cttgcaacag gcaatttagg 480
tcgatgtgct cgcacgctgt gtgaccatgt ctgagcactc cccacccacc caatcgcttc 540
ccacgtcatg ccgccacgtc gagaatttgt acacaactca ggttgcccat tttactctgt 600
tattgaacct cgcttttctg tagacccagg tatgctagaa gtaggtaatg gtagcatgac 660
cttagcagag tatcgctcac attttagcat ttgggttgtc atgaaggtaa gttttcactg 720
agattggacc gatgttgtgt catcatattt tgagtaaagg gtttgaccga agaattttga 780
aaaataaaag actgtagttc attgaaggtg ataactggta tgcaagtatt actattaatg 840
attctccact tactgatatt acatttggat aagaggaagg agatatccta attctaatgg 900
gttaactgct agcagttcct tatgtactgt tatgcttcag gcccctttat taattgcctc 960
atgtggaaag agggtgtgat gatcttttgt tgtttttgta gcgagaagct caaagaaggg 1020
gaagacatct acatctatca gggtgacaaa tccttttgca gcatggagtg cagagaaaat 1080
ttcatggtag acgagatgga aggcaaggga ggctggaagc accttttttg catgggagct 1140
gcatggagag aacttattgg tagaaaacaa actttgacca gattcttgaa tgtgccaagg 1200
gaaaaaggta ttaattggga ccttctattt atacaaggga aaaaggtatt ttttatacaa 1260
ttgctcttcc ttctcatgta tggcaacttc ctgttttgta gagattgagc gcccttcctc 1320
actgtttgaa ctttactcat cctcatgaaa ctgtgatgct acctgtagga gcggtttgtt 1380
caaggaggga atgactttta gaccctaata acttgtctac acttaagata aaaattgttg 1440
tcattgctat ggttacctcc tttagtgtcg taaactcaga ataatcatag atccctttgt 1500
tgcttacaca ttattcatgt tctacaagct attgagattt tgactagccg ctacatttta 1560
gtgcaggttt tcataatttc ttacctttat atctacattt tagatttcct cccttttgac 1620
actacatttt agccgctcac tcggaagctt tccatccctg ctggagtact cagcccataa 1680
aggcttctca ttcttatttg catggcctgt aactgctgca cagcgcgcta cggctggcgt 1740
agcggcagca actgctggta tgaacaggat ccgtccaccc tcattagcga cgatgaagtg 1800
acacccaatt ctaggcgcgg acgtacgatg gcgccctcgc gcgggatatc gggctgagtg 1860
atgagacgct gctgccattg gggttccagg cgcgcctgac cgctcccact cctaggcccg 1920
gcatgtctct ccgacgctac ctaccggtgc tggtgaaacg caaacttaaa atcgtgcatt 1980
gcaagatgtt gccttctctt tatgtctcct ctactctacg cctacgagct ttctttgttt 2040
ataacttttt ctgtggtttc gctttcaaca agctcaagcg gtcaacagat caacttagac 2100
aaaatgcaga tgcagtggaa atattaagga agaccagatt ccctcatgtt catggagcag 2160
gtgaaaagaa gtcaccagag acaattctgg atcatgagac tatgagagga ctttggcaac 2220
aaatgttcg 2229
<210> 29
<211> 579
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 29
aagttttcac tgagattgga ccgatgttgt gtcatcatat tttgagtaaa gggtttgacc 60
gaagaatttt gaaaaataaa agactgtagt tcattgaagg tgataactgg tatgcaagta 120
ttactattaa tgattctcca cttactgata ttacatttgg ataagaggaa ggagatatcc 180
taattctaat gggttaactg ctagcagttc cttatgtact gttatgcttc aggccccttt 240
attaattgcc tcatgtggaa agagggtgtg atgatctttt gttgtttttg tagcgagaag 300
ctcaaagaag gggaagacat ctacatctat cagggtgaca aatccttttg cagcatggag 360
tgcagagaaa atttcatggt agacgagatg gaaggcaagg gaggctggaa gcaccttttt 420
tgcatgggag ctgcatggag agaacttatt ggtagaaaac aaactttgac cagattcttg 480
aatgtgccaa gggaaaaagg tattaattgg gaccttctat ttatacaagg gaaaaaggta 540
ttttttatac aattgctctt ccttctcatg tatggcaac 579
<210> 30
<211> 2512
<212> DNA
<213> Zea mays
<400> 30
cccgctacct gttcaccgcg cgccagcgaa acctccgcac gcccactgcc catctgttcc 60
ccgtgcgcca gcgaaacatc cgcacgcccg cggcccgcct gttccccgcg catcccgctg 120
cacgacttct gctaccgcaa cggccaccca cgcacgcccg cctgttcacc gcgcatcccg 180
ctgacctccc cttcacgctc gcacacgctc cgttccccca ccccaccgca atccccgacg 240
ctataagagc ggtaaccaac tccatctccc tggtgccacg cattgttgag ttcttaaggt 300
gcgtttcgtt gaggacttgt tcatttttgt tggtcatgta ttccatttta ctgctctacc 360
attttgtgga ataaagggag gaatgttttc actagaagag ttcatcaatc ttatgttggt 420
ttcttggatc agttttgctc tatggctaaa tggtcgaatt gagcctattt cattataaag 480
ttagcgagcg aataattgtt cagcctcttc ctagaactca ttaccagtag aatcagttac 540
taactgcttt tctttttctt ggattagaat ggctggggct atctctcacc atgcgctagc 600
attttcacaa tcccactggt gcagtgcgaa gaactctaga ttcggaaaga ggacgggcaa 660
tgctcgcctg gtttatctaa aaggaagatg tggttcaggc agcagaaaac tgggtttgat 720
gtgggcctcg agctcgcagt cttctgtcat ggagccgacg cacctaccat ctgatggcaa 780
cagcagccac accccaaaaa aatcaagtaa ttttaacgac ctcctatggt ggttatttgt 840
ttttaatttg agaaaactat ccatttgaca catttaactt tgggcttctc agaatttggg 900
ggcatataat aagatctgct aatctgttat ctctatgtcg ttgtaggtga aagcgctctt 960
atattgattt ggcatggtga atccctgtgg aacgagaaaa atctatttcc tggctgcatc 1020
gatgtacccc tgacaccgaa gggtgttgag gaggccattg aggcaggtaa aaggatatgc 1080
aatatcccaa tcgatgtgat atatacttca tcactgattt gtgctcagat gaccgcaatg 1140
cttgccatga tgcagcatcg acgcaagaag gtttgtgtct ttcctttgaa attccagtaa 1200
tttcttctag catttgtatg aacttgccgg agaaatcatg ctttgctggt gatatatgta 1260
tttatagatc ctagttatca cgcataatga gagtgaacaa gctcacaggt ggagtcagat 1320
atacagtgag gagacaatga aacagtccat tcctgtcatc acagcttggc aattgaatga 1380
acggatgtaa tactttctcc atactctttg atttgctaat tactccctct gtctcaaaat 1440
agtattaatt ttagctcttg atttttatgt ctatattcaa atagatgatg ataaatctag 1500
attctagaca caaatataaa acatatacat caagtattat atgaatctat taatttacta 1560
agaccaattt taatttggga cagagggagt atacgattat aatagttgtt tgactgtgct 1620
tctctttaaa tatcccttga catttctagg tatggtgagc tacaaggcct taacaagcaa 1680
gaaactgtag atcgatttgg caaagaacaa gttcatgagt ggcgccgcag ttatgatatt 1740
cctccgccaa atggagaaag tctagagaag tgtgctgaga gagctgttgc ttatttcaaa 1800
gatcaggcac atctagcaag gccactttac actaattgaa agatacactt tttacttggg 1860
ttattggtct tgctgcagta ttggtatgca tgctaaaggt tattcttgaa tcgatgaatt 1920
cctctactat gggatgcaga aatgcatgtg cttagttttc tttctattgt gctagctcat 1980
atcaaattta taacctgaat tttttattta tgttcgactc taaaaaacag ttttttctag 2040
ctcgatttga cctatagtaa tttttccgta atagattatt ccacaacttg tggctggaaa 2100
acatgtgatg gttgctgcac atgggaattc acttcgttca attataatgc atctggacaa 2160
attaacttct cagaaggtaa ttcactgtcg tttttgtctt tccatcaaaa aggactcggc 2220
taaacagaac atgtagcatt atgttaagtt tgggagtgag cctttcgtcc cttcaggtaa 2280
taagccttga gctgtctact ggcattccca tgctttacat attcaaagag ggaaagttta 2340
ttcgacgtgg gactccagta ggaccttcgg aggccagtgt ttatgcttat accagggtaa 2400
gattctttcc cccacatgtt ctaccatagg acgatactcc agtttacaaa ccttatctgt 2460
acagaccaaa cgatttgctg agcacattac atttcagaac aaattggcct ag 2512
<210> 31
<211> 1005
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 31
atggctgggg ctatctctca ccatgcgcta gcattttcac aatcccactg gtgcagtgcg 60
aagaactcta gattcggaaa gaggacgggc aatgctcgcc tggtttatct aaaaggaaga 120
tgtggttcag gcagcagaaa actgggtttg atgtgggcct cgagctcgca gtcttctgtc 180
atggagccga cgcacctacc atctgatggc aacagcagcc acaccccaaa aaaatcaagt 240
gaaagcgctc ttatattgat ttggcatggt gaatccctgt ggaacgagaa aaatctattt 300
cctggctgca tcgatgtacc cctgacaccg aagggtgttg aggaggccat tgaggcaggt 360
aaaaggatat gcaatatccc aatcgatgtg atatatactt catcactgat ttgtgctcag 420
atgaccgcaa tgcttgccat gatgcagcat cgacgcaaga agatcctagt tatcacgcat 480
aatgagagtg aacaagctca caggtggagt cagatataca gtgaggagac aatgaaacag 540
tccattcctg tcatcacagc ttggcaattg aatgaacgga tgtatggtga gctacaaggc 600
cttaacaagc aagaaactgt agatcgattt ggcaaagaac aagttcatga gtggcgccgc 660
agttatgata ttcctccgcc aaatggagaa agtctagaga agtgtgctga gagagctgtt 720
gcttatttca aagatcagat tattccacaa cttgtggctg gaaaacatgt gatggttgct 780
gcacatggga attcacttcg ttcaattata atgcatctgg acaaattaac ttctcagaag 840
gtaataagcc ttgagctgtc tactggcatt cccatgcttt acatattcaa agagggaaag 900
tttattcgac gtgggactcc agtaggacct tcggaggcca gtgtttatgc ttataccagg 960
accaaacgat ttgctgagca cattacattt cagaacaaat tggcc 1005
<210> 32
<211> 394
<212> PRT
<213> Zea mays
<400> 32
Met Ala Val Val Glu Ala Cys Asp Pro Ala Arg Ile Asp Ile Ser Ser
1 5 10 15
Lys Thr Ala Ala Pro Ser Glu Arg Thr Thr Gln Leu Ile Ser Gly Gly
20 25 30
Arg His Val Ala Thr Pro Pro Leu Pro Pro Arg Ala Pro Ala Asp Ala
35 40 45
Cys Leu Ala Leu Leu Val Asp Arg Leu Ser Leu Ser Ser Val Val Ala
50 55 60
Ser Arg Leu Ser Arg Thr His Lys Asp Ala Pro Asn Val Leu Asn Leu
65 70 75 80
Ser Tyr Arg Val Arg Thr Gln Leu Ser Ala Ser Thr Leu Ile Asp Leu
85 90 95
Asn Gly Ala Phe Gly Trp Leu Ala Ile His Ile Asp Asn Leu Lys Val
100 105 110
Asn Phe Arg Cys Thr Phe Asp Gly Leu Arg Tyr Gly Ala Ala Phe Asn
115 120 125
Ser Leu Gln Cys Ala Arg Met Leu Leu Arg Arg Met Ala Glu Lys Leu
130 135 140
Arg Val Leu Leu Arg Tyr Gln Ser Leu His Val His Thr Tyr Phe Lys
145 150 155 160
Asn Ser Ser Glu Lys Leu Lys Glu Gly Glu Asp Ile Tyr Ile Tyr Gln
165 170 175
Gly Asp Lys Ser Phe Cys Ser Met Glu Cys Arg Glu Asn Phe Met Val
180 185 190
Asp Glu Met Glu Gly Lys Gly Gly Trp Lys His Leu Phe Cys Met Gly
195 200 205
Ala Ala Trp Arg Glu Leu Ile Gly Arg Lys Gln Thr Leu Thr Arg Phe
210 215 220
Leu Asn Val Pro Arg Glu Lys Gly Ile Asn Trp Asp Leu Leu Phe Ile
225 230 235 240
Gln Gly Lys Lys Ala Arg Thr Tyr Asp Gly Ala Leu Ala Arg Asp Ile
245 250 255
Gly Leu Ser Asp Glu Thr Leu Leu Pro Leu Gly Phe Gln Ala Arg Leu
260 265 270
Thr Ala Pro Thr Pro Arg Pro Gly Met Ser Leu Arg Arg Tyr Leu Pro
275 280 285
Val Leu Val Lys Arg Lys Leu Lys Ile Val His Cys Lys Met Leu Pro
290 295 300
Ser Leu Tyr Val Ser Ser Thr Leu Arg Leu Arg Ala Phe Phe Val Tyr
305 310 315 320
Asn Phe Phe Cys Gly Phe Ala Phe Asn Lys Leu Lys Arg Ser Thr Asp
325 330 335
Gln Leu Arg Gln Asn Ala Asp Ala Val Glu Ile Leu Arg Lys Thr Arg
340 345 350
Phe Pro His Val His Gly Ala Gly Glu Lys Lys Ser Pro Glu Thr Ile
355 360 365
Leu Asp His Glu Thr Met Arg Gly Leu Trp Gln Gln Met Phe Gly Leu
370 375 380
Asp Phe Ser Gly Trp Phe Tyr Asp Thr Leu
385 390
<210> 33
<211> 128
<212> PRT
<213> Zea mays
<400> 33
Met Tyr Cys Tyr Ala Ser Gly Pro Phe Ile Asn Cys Leu Met Trp Lys
1 5 10 15
Glu Gly Val Met Ile Phe Cys Cys Phe Cys Ser Glu Lys Leu Lys Glu
20 25 30
Gly Glu Asp Ile Tyr Ile Tyr Gln Gly Asp Lys Ser Phe Cys Ser Met
35 40 45
Glu Cys Arg Glu Asn Phe Met Val Asp Glu Met Glu Gly Lys Gly Gly
50 55 60
Trp Lys His Leu Phe Cys Met Gly Ala Ala Trp Arg Glu Leu Ile Gly
65 70 75 80
Arg Lys Gln Thr Leu Thr Arg Phe Leu Asn Val Pro Arg Glu Lys Gly
85 90 95
Ile Asn Trp Asp Leu Leu Phe Ile Gln Gly Lys Lys Val Phe Phe Ile
100 105 110
Gln Leu Leu Phe Leu Leu Met Tyr Gly Asn Phe Leu Phe Cys Arg Asp
115 120 125
<210> 34
<211> 335
<212> PRT
<213> Zea mays
<400> 34
Met Ala Gly Ala Ile Ser His His Ala Leu Ala Phe Ser Gln Ser His
1 5 10 15
Trp Cys Ser Ala Lys Asn Ser Arg Phe Gly Lys Arg Thr Gly Asn Ala
20 25 30
Arg Leu Val Tyr Leu Lys Gly Arg Cys Gly Ser Gly Ser Arg Lys Leu
35 40 45
Gly Leu Met Trp Ala Ser Ser Ser Gln Ser Ser Val Met Glu Pro Thr
50 55 60
His Leu Pro Ser Asp Gly Asn Ser Ser His Thr Pro Lys Lys Ser Ser
65 70 75 80
Glu Ser Ala Leu Ile Leu Ile Trp His Gly Glu Ser Leu Trp Asn Glu
85 90 95
Lys Asn Leu Phe Pro Gly Cys Ile Asp Val Pro Leu Thr Pro Lys Gly
100 105 110
Val Glu Glu Ala Ile Glu Ala Gly Lys Arg Ile Cys Asn Ile Pro Ile
115 120 125
Asp Val Ile Tyr Thr Ser Ser Leu Ile Cys Ala Gln Met Thr Ala Met
130 135 140
Leu Ala Met Met Gln His Arg Arg Lys Lys Ile Leu Val Ile Thr His
145 150 155 160
Asn Glu Ser Glu Gln Ala His Arg Trp Ser Gln Ile Tyr Ser Glu Glu
165 170 175
Thr Met Lys Gln Ser Ile Pro Val Ile Thr Ala Trp Gln Leu Asn Glu
180 185 190
Arg Met Tyr Gly Glu Leu Gln Gly Leu Asn Lys Gln Glu Thr Val Asp
195 200 205
Arg Phe Gly Lys Glu Gln Val His Glu Trp Arg Arg Ser Tyr Asp Ile
210 215 220
Pro Pro Pro Asn Gly Glu Ser Leu Glu Lys Cys Ala Glu Arg Ala Val
225 230 235 240
Ala Tyr Phe Lys Asp Gln Ile Ile Pro Gln Leu Val Ala Gly Lys His
245 250 255
Val Met Val Ala Ala His Gly Asn Ser Leu Arg Ser Ile Ile Met His
260 265 270
Leu Asp Lys Leu Thr Ser Gln Lys Val Ile Ser Leu Glu Leu Ser Thr
275 280 285
Gly Ile Pro Met Leu Tyr Ile Phe Lys Glu Gly Lys Phe Ile Arg Arg
290 295 300
Gly Thr Pro Val Gly Pro Ser Glu Ala Ser Val Tyr Ala Tyr Thr Arg
305 310 315 320
Thr Lys Arg Phe Ala Glu His Ile Thr Phe Gln Asn Lys Leu Ala
325 330 335
<210> 35
<211> 637
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 35
gctacgtgcc ttccaccaga gataagctgc gtcgtccgct ctgagggggc tgctgtcttg 60
gacgagatgt cgatccttgc ggggtcgcac gcctccacaa ccgccatgcg gcaaggttca 120
aagacaactg caactaacaa ttaaagcaca aggaatatac ttgcagatga tcattgagga 180
gcaacaaaag cttggtggat caattaaagc ttctgaggga cattgagagt gatgacaagg 240
atcttgattt tgaggttgat gatggagctg aggatgaacc taaagcatga tccgaaagat 300
gccccagccg aacctgctat caagacagtt gtggccctag ctgcgccacc caaagagaca 360
gaaagacagt tgtctaaaaa ggagatgaaa aaaaggaact agcagaactt gatgcagtat 420
tggctgagct gggactttct ggtattcgag cagcgctgca caggatggtg agagtcatac 480
atgtttccgt taaaactgcc tgatctattt tgtatgctcc ggtttagaga gagtctaaat 540
aattttgagt attttgaaat tctagatgag ttgtaaattt ccaacaagac tattgtagta 600
atagaggtta gatgttcacg tgtaggcatc acattgg 637
<210> 36
<211> 691
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 36
tatagggaga gcggccgcca gatcttccgg atggctcgag tttttcagca agatgctacg 60
tgccttccac cagagataag ctgcgtcgtc cgctctgagg gggctgctgt cttggacgag 120
atgtcgatcc ttgcggggtc gcacgcctcc acaaccgcca tgcggcaagg ttcaaagaca 180
actgcaacta acaattaaag cacaaggaat atacttgcag atgatcattg aggagcaaca 240
aaagcttggt ggatcaatta aagcttctga gggacattga gagtgatgac aaggatcttg 300
attttgaggt tgatgatgga gctgaggatg aacctaaagc atgatccgaa agatgcccca 360
gccgaacctg ctatcaagac agttgtggcc ctagctgcgc cacccaaaga gacagaaaga 420
cagttgtcta aaaaggagat gaaaaaaagg aactagcaga acttgatgca gtattggctg 480
agctgggact ttctggtatt cgagcagcgc tgcacaggat ggtgagagtc atacatgttt 540
ccgttaaaac tgcctgatct attttgtatg ctccggttta gagagagtct aaataatttt 600
gagtattttg aaattctaga tgagttgtaa atttccaaca agactattgt agtaatagag 660
gttagatgtt cacgtgtagg catcacattg g 691
<210> 37
<211> 2146
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 37
atgagaagaa tcagagacct agaggggtga gtcgggggag gggagactca cctttgtgcg 60
tgcgcgagag gcgagatgca acgacggagg agagggagag acggtcgacg aggagcgcga 120
ggcaggcgtc ggcgggagca cgaggaggga gtgggggcgt ggctacgtgc cttccaccag 180
agataagctg cgtcgtccgc tctgaggggg ctgctgtctt ggacgagatg tcgatccttg 240
cggggtcgca cgcctccaca accgccatgc ggcaaggtcg gtcctcgtgg ggccgtgcga 300
caccgaggcc gagggcggtt cccaagctgc ctgcgtcgag gccctagagc ggcggtggcg 360
acgacgttgg gggaggggcg gtggaggcaa agggaggaag ggaagcggtg gaggccgcgt 420
cggcggtgga ggccgcgagg cattctatcg cggcaggggc agggggcggg caacgcgaga 480
gagagaggaa cgtgtggggg aaggttaggg attgggtggt cgtgcggtgg agggagatag 540
agtgtgggag acgcagacgc acgatgcgat atgaacggtc cagatcgatg gatggcagaa 600
cggcagaacg agggaggcag actactattg cttacttaat aagtagtaga gatttctgtt 660
ttttacatca ttttagttta ccgacaatcc tactatgacc agaagtccta cttagttggg 720
gctacttcta caagctgata ttttggccca ttctcaatat tcacttatta actgtcacgt 780
tacacctaac agtggtttct tctcatggtt gtagtaatgc ttagttgtca ataatctatt 840
gttggcagtt ctcctgtttt gtcttattgc ttgtagttta tgagtccttt ttagcttcaa 900
gatcaatcta aattttcctt gccaactttc ttttgcttgt ctaaataagt tatgttttca 960
ggttcaaaga caactgcaac taacaattaa agcacaagga atatacttgc agatgatcat 1020
tgaggagcaa caaaagcttg gtggatcaat taaagcttct gagggtcaga aactttctga 1080
ttcacctcca agcttagatg actacccaga gatcatgcaa cattctccca agaaaccaag 1140
gatagacgca ttatcactag attcagagcg cgatatagca cagcctaaat ttgaatccca 1200
tttgatcggt ctgtgggatc acgacattgc attcccagtg gaggagttca aagcagaccc 1260
tgctatgagc aagtcataag gcaaacttca ccttgacaga aaattttcag agctgaccac 1320
ctgctaaaca aaaataaaaa atatgtacct tggataggga cttgttccaa gtttttctat 1380
atcttgggta agcaagtgat ttggaaatgg ttatacatga caggtgctgc atgtttgtca 1440
tagcaaactc tggttattgt tctacttgtt ttttgtcata atttttgcat caaaactaaa 1500
ctattaagtg gaagtataat ttactcatct cgagggggat ggagcatgtt tggctgattg 1560
tcattatagt tagcctctct gatagtgttc ttagatgtac ttctatatat gttgcaggac 1620
attgagagtg atgacaagga tcttgatttt gaggttgatg atggagctga ggatgaacct 1680
aaagcatgat ccgaaagatg ccccagccga acctgctatc aagacagttg tgggccctag 1740
ctgcgccacc caaagagaca gaaagacagt tgtctaaaaa ggagatgaaa aaaaggaact 1800
agcagaactt gatgcagtat tggctgagct gggactttct ggtattcgag cagcgctgca 1860
caggatggtg agagtcatac atgtttccgt taaaactgcc tgatctattt tgtatgctcc 1920
ggtttagaga gagtctaaat aattttgagt attttgaaat tctagatgag ttgtaaattt 1980
ccaacaagac tattgtagta atagaggtta gatgttcacg tgtaggcatc acattggtgt 2040
gcttttgggt tacccactta ggtggattgc ctgagtcatt catgaaatat tattgtgtat 2100
catcgcaaca cagtgaattg gttgttggca aaaagaattt gagaca 2146
<210> 38
<211> 637
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 38
gctacgtgcc ttccaccaga gataagctgc gtcgtccgct ctgagggggc tgctgtcttg 60
gacgagatgt cgatccttgc ggggtcgcac gcctccacaa ccgccatgcg gcaaggttca 120
aagacaactg caactaacaa ttaaagcaca aggaatatac ttgcagatga tcattgagga 180
gcaacaaaag cttggtggat caattaaagc ttctgaggga cattgagagt gatgacaagg 240
atcttgattt tgaggttgat gatggagctg aggatgaacc taaagcatga tccgaaagat 300
gccccagccg aacctgctat caagacagtt gtggccctag ctgcgccacc caaagagaca 360
gaaagacagt tgtctaaaaa ggagatgaaa aaaaggaact agcagaactt gatgcagtat 420
tggctgagct gggactttct ggtattcgag cagcgctgca caggatggtg agagtcatac 480
atgtttccgt taaaactgcc tgatctattt tgtatgctcc ggtttagaga gagtctaaat 540
agttttgagt attttgaaat tctagatgag ttgtaaattt ccaacaagac tattgtagta 600
atagaggtta gatgttcacg tgtaggcatc acattgg 637
<210> 39
<211> 55
<212> PRT
<213> Zea mays
<400> 39
Met Ala Arg Val Phe Gln Gln Asp Ala Thr Cys Leu Pro Pro Glu Ile
1 5 10 15
Ser Cys Val Val Arg Ser Glu Gly Ala Ala Val Leu Asp Glu Met Ser
20 25 30
Ile Leu Ala Gly Ser His Ala Ser Thr Thr Ala Met Arg Gln Gly Ser
35 40 45
Lys Thr Thr Ala Thr Asn Asn
50 55
<210> 40
<211> 56
<212> PRT
<213> Zea mays
<400> 40
Met Thr Arg Ile Leu Ile Leu Arg Leu Met Met Glu Leu Arg Met Asn
1 5 10 15
Leu Lys His Asp Pro Lys Asp Ala Pro Ala Glu Pro Ala Ile Lys Thr
20 25 30
Val Val Ala Leu Ala Ala Pro Pro Lys Glu Thr Glu Arg Gln Leu Ser
35 40 45
Lys Lys Glu Met Lys Lys Arg Asn
50 55
<210> 41
<211> 1327
<212> DNA
<213> Zea mays
<400> 41
tattgttgcc tcctcctcat ctcatcacta gtcactcaac cgcaattgat tgaaaattgt 60
gttcatcatc tcgttggatc gatcataatt ctttcatttc tggcctcgac aagtatcgag 120
ctcattaatc catcaatcca atgtgtgttc tgtcgaaggc gacaatggtg agctacttat 180
cgcggcgtcc atttaatggc tgcagcacaa aggcgatgga cgtgatcgtg gtcgacaaga 240
ccatcgtgcc ggggggggag gggggtagag ggtgacggtg ctgatgatgg atggcgatgg 300
tatccggggt ctcatcccgg aaaccattct tgccttcctc gaggcgaggg tgcaggatct 360
ggacaggctg gaggcgaggc tcgcagacta cttcgactac atcgccagga ccggtgggct 420
cgtcatcacg ctgctcactt cgcccggcaa ggacaagcgg cctctctacg ttgccaagaa 480
catcaaccac ttgttcttgc atccattcac atcgccctaa tcacatcaat gtatagagga 540
ctatgatgga tggatgcaag aacaatgacg ccagatggaa ttcacttttg agggaagaca 600
tgtgggctgt tctccatgta gaagtggttg atgtccttgg caacgtagag agaccgcttg 660
tttttgccgg tcccgatgaa catggcggtg atgatctcat cggtgctatt cctagtgatg 720
tagtcgaagt agtccgcgag ccttgccttc gtcccgtcca actcctacgg catggcctcg 780
aggaaggtga ggatggttcc cgagatgaga ccccagatgg cgcctctgtc caccgttatc 840
actgtcaccc tcggttcaac acgacgggct catcgaccat gctcccggcc atcgccttcg 900
tgctgcatgt gttgcatgga cgccttgatg agtagctcac cgttgccgcc tttgaccaag 960
cacacaccca attgatcgat taatgaacta gatactcatt gaggccacga atgaaagaat 1020
tatcatcaaa ccaacaaatg acggacacaa ttttcaatcg attatggatg agtgtgagta 1080
gtgatgagct gaggaagatg caatgataga tcgattgtgt acatatatag gcactgcgta 1140
cgtgctgccc ctttttggag tgacaaatag gaactagcgc gcgtattttt gcatacaacc 1200
actactaaat aagagatata tgtaaaattt aacgcaaggg atatagggaa gagatatttg 1260
tccattgcaa tgtattttga agctgtccac atatactatt tatgaagaaa cggattatgc 1320
caagtat 1327
<210> 42
<211> 714
<212> DNA
<213> Zea mays
<400> 42
atcattacat aacttatgct atattttccc gagtatgtcc taacatcttc cacagtgttt 60
ttatgggctc cttagaagtt ccagcccagg ggcctgaaac tattaaagtt ccaactgctc 120
attatgaatt tggtgccaat tttttagatc caaagttaat gctcattgga agggtgataa 180
cagatggaag gcttaatgct cgcgtgaaat gtgatttgac agacaatctc acgctgaaag 240
taaatgcaca gcttacccaa gaggcacatt actcacaagg aatgtttaac tttgactaca 300
aggttgacgt ttctgacaag tcagacgtaa cgagggcgtc cacaccgcgg ctccgccgga 360
catcgcaaca atctccccgc cccagctctc ctctccctgc gccgaggcca caatccctgc 420
cgccccggct ctcctcgtcc ccaaatcttg cacgcggtcg taatccccgc cgcctcgctc 480
tcctcgcccc tagatcgccg cctccactat cgctgatata ccagaccaag caggtagagc 540
agaccaagat gtcgctcgag gaggccaagc tggagatggc cacgctgctg cagcagcagg 600
cgagcaagtc atgcatggta ctaagtcctg catggtacta atggttgtaa tgtagtgatg 660
aaatagctag attaaaataa caaaatttat gtatggctag gatcacaaat agat 714
<210> 43
<211> 460
<212> DNA
<213> Zea mays
<400> 43
ccagcccagg ggcctgaaac tattaaagtt ccaactgctc attatgaatt tggtgccaat 60
tttttagatc caaagttaat gctcattgga agggtgataa cagatggaag gcttaatgct 120
cgcgtgaaat gtgatttgac agacaatctc acgctgaaag taaatgcaca gcttacccaa 180
gaggcacatt actcacaagg aatgtttaac tttgactaca aggacgtaac gagggcgtcc 240
acaccgcggc tccgccggac atcgcaacaa tctccccgcc ccagctctcc tctccctgcg 300
ccgaggccac aatccctgcc gccccggctc tcctcgtccc caaatcttgc acgcggtcgt 360
aatccccgcc gcctcgctct cctcgcccct agatcgccgc ctccactatc gctgatatac 420
cagaccaagc aggtagagca gaccaagatg tcgctcgagg 460
<210> 44
<211> 192
<212> PRT
<213> Zea mays
<400> 44
Met Gly Ser Leu Glu Val Pro Ala Gln Gly Pro Glu Thr Ile Lys Val
1 5 10 15
Pro Thr Ala His Tyr Glu Phe Gly Ala Asn Phe Leu Asp Pro Lys Leu
20 25 30
Met Leu Ile Gly Arg Val Ile Thr Asp Gly Arg Leu Asn Ala Arg Val
35 40 45
Lys Cys Asp Leu Thr Asp Asn Leu Thr Leu Lys Val Asn Ala Gln Leu
50 55 60
Thr Gln Glu Ala His Tyr Ser Gln Gly Met Phe Asn Phe Asp Tyr Lys
65 70 75 80
Val Asp Val Ser Asp Lys Ser Asp Val Thr Arg Ala Ser Thr Pro Arg
85 90 95
Leu Arg Arg Thr Ser Gln Gln Ser Pro Arg Pro Ser Ser Pro Leu Pro
100 105 110
Ala Pro Arg Pro Gln Ser Leu Pro Pro Arg Leu Ser Ser Ser Pro Asn
115 120 125
Leu Ala Arg Gly Arg Asn Pro Arg Arg Leu Ala Leu Leu Ala Pro Arg
130 135 140
Ser Pro Pro Pro Leu Ser Leu Ile Tyr Gln Thr Lys Gln Val Glu Gln
145 150 155 160
Thr Lys Met Ser Leu Glu Glu Ala Lys Leu Glu Met Ala Thr Leu Leu
165 170 175
Gln Gln Gln Ala Ser Lys Ser Cys Met Val Leu Ser Pro Ala Trp Tyr
180 185 190
<210> 45
<211> 127
<212> PRT
<213> Zea mays
<400> 45
Met Leu Ile Gly Arg Val Ile Thr Asp Gly Arg Leu Asn Ala Arg Val
1 5 10 15
Lys Cys Asp Leu Thr Asp Asn Leu Thr Leu Lys Val Asn Ala Gln Leu
20 25 30
Thr Gln Glu Ala His Tyr Ser Gln Gly Met Phe Asn Phe Asp Tyr Lys
35 40 45
Asp Val Thr Arg Ala Ser Thr Pro Arg Leu Arg Arg Thr Ser Gln Gln
50 55 60
Ser Pro Arg Pro Ser Ser Pro Leu Pro Ala Pro Arg Pro Gln Ser Leu
65 70 75 80
Pro Pro Arg Leu Ser Ser Ser Pro Asn Leu Ala Arg Gly Arg Asn Pro
85 90 95
Arg Arg Leu Ala Leu Leu Ala Pro Arg Ser Pro Pro Pro Leu Ser Leu
100 105 110
Ile Tyr Gln Thr Lys Gln Val Glu Gln Thr Lys Met Ser Leu Glu
115 120 125
<210> 46
<211> 2979
<212> DNA
<213> Helianthus annuus
<400> 46
atgaataaca tcaatcttgt aatagtttcg cttgtaatcg cgattgtagc catccaaccc 60
cttgcgcaag agcaaaccga tgtaggtgag gcaaatttcg tcactgttct tagcatcgat 120
ggtgggggtg ttcgtggcat tgttcccgcc accttgcttg cttttcttga atccaaaatt 180
caggtactcg aacttaaaat gcacatgtgc atcatattac aagctgtaac ttattattga 240
aatgtgccgt ctcttcggat aggaaataga tgggccagat gcacgaattg cggattattt 300
tgatgtaata gccggaacaa gcacaggagg gctgatgaca actatgcttg cagctcctaa 360
tgagaaaaat cgtcccatgt tcgccgcaaa agacattacc aacttctact ttcaacattc 420
gcctaggatc ttccctaaaa tagggtaaac tctaactagt ttccggatct ataagatcat 480
cattaaatac aagtttcatt ttctttttcg aatcaaatac agacacacat ttgatgaggc 540
gcaaccttat ccttctcaaa acgaagcctg cgaaatgggg tattctccta caaagacttt 600
tgtaattcat gttctagtgg gtgtttggat gtgcgtttta aaactgatta ttatttacat 660
gtagtttctg aagaaaataa aacagttatt caaacacttt ttgttaataa ttctactaga 720
aaaaaaaaat ccttgtcagg aaattaatta aaaaaaagtt accatctatt aaagttcttt 780
cttactaatc aaaagttttt aaattttatt atcatgttat tataactaaa catacacatc 840
caaacactat ctcataccac atgattacac aagtctatta tttgaatatg ctaacttagt 900
attttcatat aataagtttt taaaacgcca catccaaaac ccttgattct tattttacat 960
tgtgtagcta aaacagtgtt tatacataaa aacaatcagt tatataaatc aaagcattat 1020
ttaactaaag taagctcggt tcaaactcga taagagaatt aatatatacg agtcgagttc 1080
ctgttgacca gatttcgcta gtgttaagtt tcgagttcaa aattgtatat gaacttgaac 1140
ctgtgtatca tcatacttga catttaaacc ataatgttgt tgaataaata aagtgatttt 1200
attttgtagt cggaccaaat tcatgaattc ggtagtaacc gtacttggtg aggccaccgg 1260
accaaagtat gatggtaaat atcttcgagc catggcaaag atgatgttaa aaaacctcac 1320
tattaaagat acgttgacga atgttgtcat acctgctttc gacattaggc ggcttcaacc 1380
tgttatcttc tcctctgctc aagtaattaa actcgttttt tatatttata gcagttctct 1440
atttaaaatt gattgtgtat cataaaatgg tttctgtttg atacgtttag ggaaaagagg 1500
tcgcgtggaa aaatgctttg ctagcagacg tatgcattag taccgcggcg gcaccaacat 1560
ttttcccgcc atactatttt gagactagag acgtcgatgg aaccaagcac acttttgatc 1620
taatcgatgg cggggtagct gcaaacaatc cggtagttac atttcaacaa tattgagttt 1680
gcattttatt tttaggacaa gtagtcacat tagggtgaag ggtgtgttca agctcatccc 1740
gaaggtggga gcggtgttcc cactcgtacc tcatggcgca ttttcttctt tgttgcagct 1800
ccaattttaa aaagccaccc cgccttttcc attccatagc gccacgtcaa ctgggaaatg 1860
gtgttcccac tggtattgga gatttggagg cgctacgcca cctctgtcat cccgaagcca 1920
caccctccac ccttaggagt gttatcggtt cagttttcgg tttatacagt ttaaaggttt 1980
ttttttggtt gaaaccaaaa accgaactga actgaacgga attcgggtag ttcacaactg 2040
aaccaaaaac tgaatccata ttcggttttc tgtttgaccg aataatcatt atttgttatt 2100
tgattgtgcg aggttaaata agattgagca aaaattgtaa ttaattggat ttggctaaat 2160
gacaatttaa ataaccgtta tttgttatcc gattcaaacc gaacacccaa ctcgatttgg 2220
tttaataccg aaccgacaac cggatacgta attcagttcg ctattaaaag gttcgggttt 2280
ggtagggttt aacccatttg aaatcgaatc ttcagagaaa gggttcgcta atgtcaccca 2340
cataaccaaa gaaatcttgt ttaaatgtta atggcagaca catttggcta tcacacatat 2400
aaccaaagaa gcggtgatgg ggaaatacag gttctctggc ccggaggttt tcgacggaag 2460
acggatgctt gtgctttcac tcggcactgg tacgcagacg tacaatgact tatatactgc 2520
acaaaaggct gcaaaatggg ggttgcttag ttggatcttt accaatggta ctgcgccaat 2580
cctccgcatt tttggtgatg ccatgtcaga tatggtcgac atccatgtgt caactatatt 2640
ccaatcgttg caagtcgaaa aaaactatct gcgtattcag gtataactaa gaacatataa 2700
atataatgtt gtataggtta catgtttagt aacaaggagt ttttttatgg gcaggaagat 2760
aacttgaaag gggaagcaac tgcaatggat atttcatcac ctgagaacat gagggcgcta 2820
gaggacattg gcaagaaatt gttgaagaaa ccgttgtcga gattggatgt ggagacaggc 2880
aagcttgaac cagttaaagg agaaggtacg aatgctgatg cattagcacg tttcgccact 2940
ttgctttgtg ccgaacgaaa gcgccgcaat ccagcttaa 2979
<210> 47
<211> 1302
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA
<400> 47
atgaataaca tcaatcttgt aatagtttcg cttgtaatcg cgattgtagc catccaaccc 60
cttgcgcaag agcaaaccga tgtaggtgag gcaaatttcg tcactgttct tagcatcgat 120
ggtgggggtg ttcgtggcat tgttcccgcc accttgcttg cttttcttga atccaaaatt 180
caggaaatag atgggccaga tgcacgaatt gcggattatt ttgatgtaat agccggaaca 240
agcacaggag ggctgatgac aactatgctt gcagctccta atgagaaaaa tcgtcccatg 300
ttcgccgcaa aagacattac caacttctac tttcaacatt cgcctaggat cttccctaaa 360
ataggacaca catttgatga ggcgcaacct tatccttctc aaaacgaagc ctgcgaaatg 420
ggtcggacca aattcatgaa ttcggtagta accgtacttg gtgaggccac cggaccaaag 480
tatgatggta aatatcttcg agccatggca aagatgatgt taaaaaacct cactattaaa 540
gatacgttga cgaatattgt catacctgct ttcgacatca ggcggcttca acctgttatc 600
ttctcctctg ctcaaggaaa agaggtcgcg tggaaaaatg ctttgctagc agacgtatgc 660
attagtaccg cggcggcacc aacgtttttc ccgccatact attttgagac tagagatgtc 720
gatggaacca agcacacttt tgatctaatc gatggcgggg tagctgcaaa caatccgaca 780
catttggcta tcacacatat aaccaaagaa gcggtgatgg ggaaatacag gttctctggc 840
ccggaggttt tcgacggcag acggatgctt gtgctttcac tcggcactgg tacgcagacg 900
tacaatgact tatacactgc acaaaaggct gcaaaatggg ggttgcttag ttggatcttt 960
accaatggta ctgcgccaat cctccgcatt tttggtgatg ccatgtcaga tatggtcgac 1020
atccatgtgt caactatatt ccaatcgttg caagtcgaaa aaaactatct gcgtattcag 1080
gaagataact tgaaagggga agcaactgca atggatattt catcacccga gaacatgagg 1140
gcgctagagg acattggcaa gaaattgttg aagaaaccgt tgtcgagatt ggatgtggag 1200
acaggcaagc ttgaaccagt taaaggagaa ggtacgaatg ctgatgcatt agcacgtttc 1260
gccactttgc tttgtgccga acgaaagcgc cgcaatccag ct 1302
<210> 48
<211> 433
<212> PRT
<213> Helianthus annuus
<400> 48
Met Asn Asn Ile Asn Leu Val Ile Val Ser Leu Val Ile Ala Ile Val
1 5 10 15
Ala Ile Gln Pro Leu Ala Gln Glu Gln Thr Asp Val Gly Glu Ala Asn
20 25 30
Phe Val Thr Val Leu Ser Ile Asp Gly Gly Gly Val Arg Gly Ile Val
35 40 45
Pro Ala Thr Leu Leu Ala Phe Leu Glu Ser Lys Ile Gln Glu Ile Asp
50 55 60
Gly Pro Asp Ala Arg Ile Ala Asp Tyr Phe Asp Val Ile Ala Gly Thr
65 70 75 80
Ser Thr Gly Gly Leu Met Thr Thr Met Leu Ala Ala Pro Asn Glu Lys
85 90 95
Asn Arg Pro Met Phe Ala Ala Lys Asp Ile Thr Asn Phe Tyr Phe Gln
100 105 110
His Ser Pro Arg Ile Phe Pro Lys Ile Gly His Thr Phe Asp Glu Ala
115 120 125
Gln Pro Tyr Pro Ser Gln Asn Glu Ala Cys Glu Met Gly Arg Thr Lys
130 135 140
Phe Met Asn Ser Val Val Thr Val Leu Gly Glu Ala Thr Gly Pro Lys
145 150 155 160
Tyr Asp Gly Lys Tyr Leu Arg Ala Met Ala Lys Met Met Leu Lys Asn
165 170 175
Leu Thr Ile Lys Asp Thr Leu Thr Asn Ile Val Ile Pro Ala Phe Asp
180 185 190
Ile Arg Arg Leu Gln Pro Val Ile Phe Ser Ser Ala Gln Gly Lys Glu
195 200 205
Val Ala Trp Lys Asn Ala Leu Leu Ala Asp Val Cys Ile Ser Thr Ala
210 215 220
Ala Ala Pro Thr Phe Phe Pro Pro Tyr Tyr Phe Glu Thr Arg Asp Val
225 230 235 240
Asp Gly Thr Lys His Thr Phe Asp Leu Ile Asp Gly Gly Val Ala Ala
245 250 255
Asn Asn Pro Thr His Leu Ala Ile Thr His Ile Thr Lys Glu Ala Val
260 265 270
Met Gly Lys Tyr Arg Phe Ser Gly Pro Glu Val Phe Asp Gly Arg Arg
275 280 285
Met Leu Val Leu Ser Leu Gly Thr Gly Thr Gln Thr Tyr Asn Asp Leu
290 295 300
Tyr Thr Ala Gln Lys Ala Ala Lys Trp Gly Leu Leu Ser Trp Ile Phe
305 310 315 320
Thr Asn Gly Thr Ala Pro Ile Leu Arg Ile Phe Gly Asp Ala Met Ser
325 330 335
Asp Met Val Asp Ile His Val Ser Thr Ile Phe Gln Ser Leu Gln Val
340 345 350
Glu Lys Asn Tyr Leu Arg Ile Gln Glu Asp Asn Leu Lys Gly Glu Ala
355 360 365
Thr Ala Met Asp Ile Ser Ser Pro Glu Asn Met Arg Ala Leu Glu Asp
370 375 380
Ile Gly Lys Lys Leu Leu Lys Lys Pro Leu Ser Arg Leu Asp Val Glu
385 390 395 400
Thr Gly Lys Leu Glu Pro Val Lys Gly Glu Gly Thr Asn Ala Asp Ala
405 410 415
Leu Ala Arg Phe Ala Thr Leu Leu Cys Ala Glu Arg Lys Arg Arg Asn
420 425 430
Pro
<210> 49
<211> 1795
<212> DNA
<213> Artificial Sequence
<220>
<223> TILLING mutant D74N
<400> 49
agttcatcac taatcacact tattgtgccc tcgacgagta tctatagcta gctcattaat 60
cgattcgggg gtgtgttgtc gaaggcggca atggcgagct actcgtcgcg gcgtccatgc 120
aatacctgta gcacgaaggc gatggccggg agcgtggtcg gcgagcccgt cgtgctgggg 180
cagagggtga cggtgctgac ggtggacggc ggcggcgtcc ggggtctcat cccgggaacc 240
atcctcgcct tcctggaggc caggctgcag gagctggacg gaccggaggc gaggctggcg 300
gactacttca actacatcgc cggaaccagc accggcggtc tcatcaccgc catgctcacc 360
gcgcccggca aggacaagcg gcctctctac gctgccaagg acatcaacca cttttacatg 420
cagaactgcc cgcgcatctt tcctcagaag tgagtccgat gctgccgcca ttgttcttgc 480
atccatccag catcgtacgt acgtcctcta tacatctgcg gatcatcatg tgcgcatgtt 540
tgtggcatgc atgcatgcat gtgagcagga gcaggcttgc ggccgccatg tccgcgctga 600
ggaagccaaa gtacaacggc aagtgcatgc gcagcctgat taggagcatc ctcggcgaga 660
cgagggtaag cgagacgctg accaacgtca tcatccctgc cttcgacatc aggctgctgc 720
agcctatcat cttctctacc tacgacgtac gtacgtcgtc acgaatgatt catctgtacg 780
tcgtcgcatg cgaatggctg cctacgtacg ccgtgcgcta acatactcag ctctttccta 840
tctgctgcgc caatttgcag gccaagagca cgcctctgaa gaacgctctg ctctcggacg 900
tgtgcattgg cacgtccgcc gcgccgacct acctcccggc gcactacttc cagactgaag 960
acgccaacgg caaggagcgc gaatacaacc tcatcgacgg cggtgtggcg gccaacaacc 1020
cggtaactga ctagctaact ggaaaacgga cgcacagact ccatgtccat ggcggcccac 1080
aaggtcgatg ctaattgttg cttatgtatg tcgcccgatt gcacatgcgt agacgatggt 1140
tgcgatgacg cagatcacca aaaagatgct tgccagcaag gacaaggccg aggagctgta 1200
cccagtgaag ccgtcgaact gccgcaggtt cctggtgctg tccatcggga cggggtcgac 1260
gtccgagcag ggcctctaca cggcgcggca gtgctcccgg tggggtatct gccggtggct 1320
ccgcaacaac ggcatggccc ccatcatcga catcttcatg gcggccagct cggacctggt 1380
ggacatccac gtcgccgcga tgttccagtc gctccacagc gacggcgact acctgcgcat 1440
ccaggacaac tcgctccgtg gcgccgcggc caccgtggac gcggcgacgc cggagaacat 1500
gcggacgctc gtcgggatcg gggagcggat gctggcacag agggtgtcca gggtcaacgt 1560
ggagacaggg aggtacgaac cggtgactgg cgaaggaagc aatgccgatg ccctcggtgg 1620
gctcgctagg cagctctccg aggagaggag aacaaggctc gcgcgccgcg tctctgccat 1680
caacccaaga ggctctagat gtgcgtcgta cgatatctaa gacaagtggc tttactgtca 1740
gtcacatgct tgtaaataag tagactttat tttaataaaa cataaaaata tatat 1795
<210> 50
<211> 1284
<212> DNA
<213> Artificial Sequence
<220>
<223> TILLING mutant D74N
<400> 50
atggcgagct actcgtcgcg gcgtccatgc aatacctgta gcacgaaggc gatggccggg 60
agcgtggtcg gcgagcccgt cgtgctgggg cagagggtga cggtgctgac ggtggacggc 120
ggcggcgtcc ggggtctcat cccgggaacc atcctcgcct tcctggaggc caggctgcag 180
gagctggacg gaccggaggc gaggctggcg gactacttca actacatcgc cggaaccagc 240
accggcggtc tcatcaccgc catgctcacc gcgcccggca aggacaagcg gcctctctac 300
gctgccaagg acatcaacca cttttacatg cagaactgcc cgcgcatctt tcctcagaag 360
agcaggcttg cggccgccat gtccgcgctg aggaagccaa agtacaacgg caagtgcatg 420
cgcagcctga ttaggagcat cctcggcgag acgagggtaa gcgagacgct gaccaacgtc 480
atcatccctg ccttcgacat caggctgctg cagcctatca tcttctctac ctacgacgcc 540
aagagcacgc ctctgaagaa cgctctgctc tcggacgtgt gcattggcac gtccgccgcg 600
ccgacctacc tcccggcgca ctacttccag actgaagacg ccaacggcaa ggagcgcgaa 660
tacaacctca tcgacggcgg tgtggcggcc aacaacccga cgatggttgc gatgacgcag 720
atcaccaaaa agatgcttgc cagcaaggac aaggccgagg agctgtaccc agtgaagccg 780
tcgaactgcc gcaggttcct ggtgctgtcc atcgggacgg ggtcgacgtc cgagcagggc 840
ctctacacgg cgcggcagtg ctcccggtgg ggtatctgcc ggtggctccg caacaacggc 900
atggccccca tcatcgacat cttcatggcg gccagctcgg acctggtgga catccacgtc 960
gccgcgatgt tccagtcgct ccacagcgac ggcgactacc tgcgcatcca ggacaactcg 1020
ctccgtggcg ccgcggccac cgtggacgcg gcgacgccgg agaacatgcg gacgctcgtc 1080
gggatcgggg agcggatgct ggcacagagg gtgtccaggg tcaacgtgga gacagggagg 1140
tacgaaccgg tgactggcga aggaagcaat gccgatgccc tcggtgggct cgctaggcag 1200
ctctccgagg agaggagaac aaggctcgcg cgccgcgtct ctgccatcaa cccaagaggc 1260
tctagatgtg cgtcgtacga tatc 1284
<210> 51
<211> 428
<212> PRT
<213> Artificial Sequence
<220>
<223> TILLING mutant D74N
<400> 51
Met Ala Ser Tyr Ser Ser Arg Arg Pro Cys Asn Thr Cys Ser Thr Lys
1 5 10 15
Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln Arg
20 25 30
Val Thr Val Leu Thr Val Asp Gly Gly Gly Val Arg Gly Leu Ile Pro
35 40 45
Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp Gly
50 55 60
Pro Glu Ala Arg Leu Ala Asp Tyr Phe Asn Tyr Ile Ala Gly Thr Ser
65 70 75 80
Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp Lys
85 90 95
Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn His Phe Tyr Met Gln Asn
100 105 110
Cys Pro Arg Ile Phe Pro Gln Lys Ser Arg Leu Ala Ala Ala Met Ser
115 120 125
Ala Leu Arg Lys Pro Lys Tyr Asn Gly Lys Cys Met Arg Ser Leu Ile
130 135 140
Arg Ser Ile Leu Gly Glu Thr Arg Val Ser Glu Thr Leu Thr Asn Val
145 150 155 160
Ile Ile Pro Ala Phe Asp Ile Arg Leu Leu Gln Pro Ile Ile Phe Ser
165 170 175
Thr Tyr Asp Ala Lys Ser Thr Pro Leu Lys Asn Ala Leu Leu Ser Asp
180 185 190
Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala His Tyr
195 200 205
Phe Gln Thr Glu Asp Ala Asn Gly Lys Glu Arg Glu Tyr Asn Leu Ile
210 215 220
Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala Met Thr Gln
225 230 235 240
Ile Thr Lys Lys Met Leu Ala Ser Lys Asp Lys Ala Glu Glu Leu Tyr
245 250 255
Pro Val Lys Pro Ser Asn Cys Arg Arg Phe Leu Val Leu Ser Ile Gly
260 265 270
Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg Gln Cys Ser
275 280 285
Arg Trp Gly Ile Cys Arg Trp Leu Arg Asn Asn Gly Met Ala Pro Ile
290 295 300
Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp Ile His Val
305 310 315 320
Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr Leu Arg Ile
325 330 335
Gln Asp Asn Ser Leu Arg Gly Ala Ala Ala Thr Val Asp Ala Ala Thr
340 345 350
Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg Met Leu Ala
355 360 365
Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr Glu Pro Val
370 375 380
Thr Gly Glu Gly Ser Asn Ala Asp Ala Leu Gly Gly Leu Ala Arg Gln
385 390 395 400
Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg Arg Val Ser Ala Ile
405 410 415
Asn Pro Arg Gly Ser Arg Cys Ala Ser Tyr Asp Ile
420 425
<210> 52
<211> 1795
<212> DNA
<213> Artificial Sequence
<220>
<223> TILLING mutant G78R
<400> 52
agttcatcac taatcacact tattgtgccc tcgacgagta tctatagcta gctcattaat 60
cgattcgggg gtgtgttgtc gaaggcggca atggcgagct actcgtcgcg gcgtccatgc 120
aatacctgta gcacgaaggc gatggccggg agcgtggtcg gcgagcccgt cgtgctgggg 180
cagagggtga cggtgctgac ggtggacggc ggcggcgtcc ggggtctcat cccgggaacc 240
atcctcgcct tcctggaggc caggctgcag gagctggacg gaccggaggc gaggctggcg 300
gactacttcg actacatcgc cagaaccagc accggcggtc tcatcaccgc catgctcacc 360
gcgcccggca aggacaagcg gcctctctac gctgccaagg acatcaacca cttttacatg 420
cagaactgcc cgcgcatctt tcctcagaag tgagtccgat gctgccgcca ttgttcttgc 480
atccatccag catcgtacgt acgtcctcta tacatctgcg gatcatcatg tgcgcatgtt 540
tgtggcatgc atgcatgcat gtgagcagga gcaggcttgc ggccgccatg tccgcgctga 600
ggaagccaaa gtacaacggc aagtgcatgc gcagcctgat taggagcatc ctcggcgaga 660
cgagggtaag cgagacgctg accaacgtca tcatccctgc cttcgacatc aggctgctgc 720
agcctatcat cttctctacc tacgacgtac gtacgtcgtc acgaatgatt catctgtacg 780
tcgtcgcatg cgaatggctg cctacgtacg ccgtgcgcta acatactcag ctctttccta 840
tctgctgcgc caatttgcag gccaagagca cgcctctgaa gaacgctctg ctctcggacg 900
tgtgcattgg cacgtccgcc gcgccgacct acctcccggc gcactacttc cagactgaag 960
acgccaacgg caaggagcgc gaatacaacc tcatcgacgg cggtgtggcg gccaacaacc 1020
cggtaactga ctagctaact ggaaaacgga cgcacagact ccatgtccat ggcggcccac 1080
aaggtcgatg ctaattgttg cttatgtatg tcgcccgatt gcacatgcgt agacgatggt 1140
tgcgatgacg cagatcacca aaaagatgct tgccagcaag gacaaggccg aggagctgta 1200
cccagtgaag ccgtcgaact gccgcaggtt cctggtgctg tccatcggga cggggtcgac 1260
gtccgagcag ggcctctaca cggcgcggca gtgctcccgg tggggtatct gccggtggct 1320
ccgcaacaac ggcatggccc ccatcatcga catcttcatg gcggccagct cggacctggt 1380
ggacatccac gtcgccgcga tgttccagtc gctccacagc gacggcgact acctgcgcat 1440
ccaggacaac tcgctccgtg gcgccgcggc caccgtggac gcggcgacgc cggagaacat 1500
gcggacgctc gtcgggatcg gggagcggat gctggcacag agggtgtcca gggtcaacgt 1560
ggagacaggg aggtacgaac cggtgactgg cgaaggaagc aatgccgatg ccctcggtgg 1620
gctcgctagg cagctctccg aggagaggag aacaaggctc gcgcgccgcg tctctgccat 1680
caacccaaga ggctctagat gtgcgtcgta cgatatctaa gacaagtggc tttactgtca 1740
gtcacatgct tgtaaataag tagactttat tttaataaaa cataaaaata tatat 1795
<210> 53
<211> 1284
<212> DNA
<213> Artificial Sequence
<220>
<223> TILLING mutant G78R
<400> 53
atggcgagct actcgtcgcg gcgtccatgc aatacctgta gcacgaaggc gatggccggg 60
agcgtggtcg gcgagcccgt cgtgctgggg cagagggtga cggtgctgac ggtggacggc 120
ggcggcgtcc ggggtctcat cccgggaacc atcctcgcct tcctggaggc caggctgcag 180
gagctggacg gaccggaggc gaggctggcg gactacttcg actacatcgc cagaaccagc 240
accggcggtc tcatcaccgc catgctcacc gcgcccggca aggacaagcg gcctctctac 300
gctgccaagg acatcaacca cttttacatg cagaactgcc cgcgcatctt tcctcagaag 360
agcaggcttg cggccgccat gtccgcgctg aggaagccaa agtacaacgg caagtgcatg 420
cgcagcctga ttaggagcat cctcggcgag acgagggtaa gcgagacgct gaccaacgtc 480
atcatccctg ccttcgacat caggctgctg cagcctatca tcttctctac ctacgacgcc 540
aagagcacgc ctctgaagaa cgctctgctc tcggacgtgt gcattggcac gtccgccgcg 600
ccgacctacc tcccggcgca ctacttccag actgaagacg ccaacggcaa ggagcgcgaa 660
tacaacctca tcgacggcgg tgtggcggcc aacaacccga cgatggttgc gatgacgcag 720
atcaccaaaa agatgcttgc cagcaaggac aaggccgagg agctgtaccc agtgaagccg 780
tcgaactgcc gcaggttcct ggtgctgtcc atcgggacgg ggtcgacgtc cgagcagggc 840
ctctacacgg cgcggcagtg ctcccggtgg ggtatctgcc ggtggctccg caacaacggc 900
atggccccca tcatcgacat cttcatggcg gccagctcgg acctggtgga catccacgtc 960
gccgcgatgt tccagtcgct ccacagcgac ggcgactacc tgcgcatcca ggacaactcg 1020
ctccgtggcg ccgcggccac cgtggacgcg gcgacgccgg agaacatgcg gacgctcgtc 1080
gggatcgggg agcggatgct ggcacagagg gtgtccaggg tcaacgtgga gacagggagg 1140
tacgaaccgg tgactggcga aggaagcaat gccgatgccc tcggtgggct cgctaggcag 1200
ctctccgagg agaggagaac aaggctcgcg cgccgcgtct ctgccatcaa cccaagaggc 1260
tctagatgtg cgtcgtacga tatc 1284
<210> 54
<211> 428
<212> PRT
<213> Artificial Sequence
<220>
<223> TILLING mutant G78R
<400> 54
Met Ala Ser Tyr Ser Ser Arg Arg Pro Cys Asn Thr Cys Ser Thr Lys
1 5 10 15
Ala Met Ala Gly Ser Val Val Gly Glu Pro Val Val Leu Gly Gln Arg
20 25 30
Val Thr Val Leu Thr Val Asp Gly Gly Gly Val Arg Gly Leu Ile Pro
35 40 45
Gly Thr Ile Leu Ala Phe Leu Glu Ala Arg Leu Gln Glu Leu Asp Gly
50 55 60
Pro Glu Ala Arg Leu Ala Asp Tyr Phe Asp Tyr Ile Ala Arg Thr Ser
65 70 75 80
Thr Gly Gly Leu Ile Thr Ala Met Leu Thr Ala Pro Gly Lys Asp Lys
85 90 95
Arg Pro Leu Tyr Ala Ala Lys Asp Ile Asn His Phe Tyr Met Gln Asn
100 105 110
Cys Pro Arg Ile Phe Pro Gln Lys Ser Arg Leu Ala Ala Ala Met Ser
115 120 125
Ala Leu Arg Lys Pro Lys Tyr Asn Gly Lys Cys Met Arg Ser Leu Ile
130 135 140
Arg Ser Ile Leu Gly Glu Thr Arg Val Ser Glu Thr Leu Thr Asn Val
145 150 155 160
Ile Ile Pro Ala Phe Asp Ile Arg Leu Leu Gln Pro Ile Ile Phe Ser
165 170 175
Thr Tyr Asp Ala Lys Ser Thr Pro Leu Lys Asn Ala Leu Leu Ser Asp
180 185 190
Val Cys Ile Gly Thr Ser Ala Ala Pro Thr Tyr Leu Pro Ala His Tyr
195 200 205
Phe Gln Thr Glu Asp Ala Asn Gly Lys Glu Arg Glu Tyr Asn Leu Ile
210 215 220
Asp Gly Gly Val Ala Ala Asn Asn Pro Thr Met Val Ala Met Thr Gln
225 230 235 240
Ile Thr Lys Lys Met Leu Ala Ser Lys Asp Lys Ala Glu Glu Leu Tyr
245 250 255
Pro Val Lys Pro Ser Asn Cys Arg Arg Phe Leu Val Leu Ser Ile Gly
260 265 270
Thr Gly Ser Thr Ser Glu Gln Gly Leu Tyr Thr Ala Arg Gln Cys Ser
275 280 285
Arg Trp Gly Ile Cys Arg Trp Leu Arg Asn Asn Gly Met Ala Pro Ile
290 295 300
Ile Asp Ile Phe Met Ala Ala Ser Ser Asp Leu Val Asp Ile His Val
305 310 315 320
Ala Ala Met Phe Gln Ser Leu His Ser Asp Gly Asp Tyr Leu Arg Ile
325 330 335
Gln Asp Asn Ser Leu Arg Gly Ala Ala Ala Thr Val Asp Ala Ala Thr
340 345 350
Pro Glu Asn Met Arg Thr Leu Val Gly Ile Gly Glu Arg Met Leu Ala
355 360 365
Gln Arg Val Ser Arg Val Asn Val Glu Thr Gly Arg Tyr Glu Pro Val
370 375 380
Thr Gly Glu Gly Ser Asn Ala Asp Ala Leu Gly Gly Leu Ala Arg Gln
385 390 395 400
Leu Ser Glu Glu Arg Arg Thr Arg Leu Ala Arg Arg Val Ser Ala Ile
405 410 415
Asn Pro Arg Gly Ser Arg Cys Ala Ser Tyr Asp Ile
420 425
<210> 55
<211> 13516
<212> DNA
<213> Zea mays
<400> 55
tcttgctata tatgagatga caaaattttc caaagaagag agaagccggc agaacccatc 60
ctgtttcaaa tctcttctac tacttaagtt tctaacgtag gcgtcgacaa aacggattgg 120
tgcacggttc tgccgatgtc tcccacacac gcgcatggaa ggaggcaggc acccttcccc 180
gccgccccgg atctcgcgcc agccccagcc ctaccccgcc tgcccttcca ttcttcccca 240
gccgcccccc ggtcaacgtc acgaacccgg gcctcgtgcc gttcgccgtg gccacgcggt 300
tcgacgagcg ggtcacggag ctgctgagcg cgctcgctga cgcggcggcg gggcgaccag 360
gcaggtgggc catcggcgaa gcgccatggt cgtcgtcggg gggcaggaac caggcggtgt 420
acgcgcgccg cgcgcccggc tcttcatcgc ctccacccgc tccagcgtct ccaccacctc 480
cttcatcgag ggccgactgc gaggctcgcc ggccaggcag ccgagcgtca gttgcgccgc 540
ttggaacgcc tgcttttgtt gatcgtttgt tttggtctga tttcggtggg tctatccgca 600
gagaggaaga agcagaagct ctccgagatc caatccggcg ttgaggaagc tgaatcgctg 660
gtaaatagat gccgcgacac gttctggttt ggggatcccc ttggctaaca ggacatacga 720
catttgggga atgggtagaa aagcagagat tagggatttt tcgtttccgt cggtgcagtt 780
ttggtgttcc aacggagttg cgagatgttt atgtgcctta gtcttcaatt tgggggttgg 840
gggaaaagta attttatgtt tttgttttgt gtctgcagat tcagaaaatg gacctggagg 900
caaggagcct acagcctagc attaaggcta gtttgcttgc aaagctgagg gagtataaat 960
ctgacctcaa caacgtcaag agtgagctca agaggatatc tgcgcccaat gccaggcagg 1020
ctacccggga ggagctcctg gagtctggaa tggctgatac tctcgcagtg agctaatgat 1080
aggacttgac tgtgtctacg agactgctcc taacaataaa ctgaagaaag caaaagaaat 1140
cattcaacgt attcgccgaa gagaactcta caaggtagta tgatgcttta attgctcata 1200
tacaagtgtc attttgtcat gtcattacac atggttagga tacataggag attctgtttt 1260
ttaacacata gttgtcccat gtccatgaat tcatttgaat taatttactc ttcgcaatct 1320
tatacattaa aatcgtgtta cctattacat cacaacttca tgagagcatg cttgttctgt 1380
gtagatatgg tagtcctggg aggaagagcc atcgccgcta tgtatggcag aaccacccgc 1440
gaaagcaacc tctctggtct cgcatagcca gagcaggagc agctcgcttg cgcggccgca 1500
gcgctggcgg tcggccccgc gtacgagcgc ctgcaggtag gccagcttct gctgcaatgc 1560
ccgaatctcg gcgtccacgc gcagcagcgt cgtcgcctcc tcctccgtca gctcacccag 1620
cttggccagc acccccgtca cccccgcgtc cgccatggct gtcgccggga ccgaaaggct 1680
aaaactgtca caatgacgta aagtttggtt ggtgttggcg gctcacgcaa aaccagacct 1740
ttccaagttt tactttagca gagttttttt ggaacgagag caaagcagca cagtttcaag 1800
aatgtggggc aatttgaatg ttcgttcctg ctgcactgct actgctttta gaattgtagt 1860
atgcttcatc atttatttat ttctaaaaaa acttgcatga attctatcgt gacttttatt 1920
gagaaaataa tgtattcacg tatcttcatg tttctgataa aggtatttgt atatgcatcg 1980
gtgctacata tgcgaataca agttttgttt caactctgaa gtctcaagtt gaattctaaa 2040
ctccagtttg ttttctactg tgctgctgca ggaagccagg aacccatccg aacaaggttg 2100
caatcatgat aagcagatag agcaagcata tgatgatatt ttgaattcgt cgaagcatac 2160
tttggccagc atgatggagc tgcaggaggt tcagttattt gcacacattg tttttctttc 2220
actcctatga ttttcctcaa tatgatcaaa atgtttcttt tgcaaataat gattgaaatg 2280
tttctcattg tactcaacct cttaaactac ctataggctt tgcttgagag taatcaggct 2340
acaaaggatg ccaatggtat tgctgctctc tatattgttc ttgttctaat gtaaaaacta 2400
caacacaact ctttacttga tcccagaaat tccttctgcc tcaaatggag acaatgacga 2460
gtggtcagaa gtacagagat tgcagacaag gtaaattttg caatagaaat aactaaccaa 2520
ccattagtgc ttgaaaaaaa ctggactggt gactggggca cgtggtttca tcaacatttg 2580
gacctcaacg gtctaatcag tataacttag aagttggcta gctcttgaaa aacactgcat 2640
gacactaagc atttgtttat tttcagctgc ttgcacccct atgatttcaa gtaactactt 2700
gtctacttgt gataatcacc tgaatatgag tatttgaaat gcttatcacg tctcggcaat 2760
tgcatttctt ttatgcgtaa ctgaagtctg ctctagcttc ctaatagagt tcatttttta 2820
atacagaaac cactttgaga tagccacaat atagtaaaag tggcagctaa ggtactaaaa 2880
acacccatgc aaataagaaa aaaaatgaat cttgtatttt aattttgtta aatacctcta 2940
tagtttggcg atatattatg ttaccatcct gcttgtagcc tgtaggtcat tttatatgag 3000
ccatcaaatt gcgatgacag ttgccacaaa tccagtttca tatgaaggta ttagctgtgt 3060
aacaagctaa ctgctgctct ctgcccaata agttattcaa ttggattagt aggttgcatc 3120
caaggttatt caattggatc agtaggttgc atccaaggta tactgctgct ctctgcccaa 3180
taagttattc aattcgatca gtaggttgcc tgttcccttc attttattaa aaaatacata 3240
ataatataat aagtacctgt ttgttctaaa aataatactt ctgtaaatga ggttattaat 3300
tttccttttg gtaataatgc aggttgatga tactgaagtc atcagttttt tgttgcaaac 3360
tgaaataatt tctctgtgct tgcgaaccat ggagatgggt agtgagctat ccaaaactgt 3420
atgtagctag ccatatattc tcattcaaat atcataattt atctcttctg cttaatactg 3480
gcaaaggtgt aatagttttt ttagtattga tttgtcacct gaagtttatc ttgtgcacta 3540
ctactttgcc atcatcagtt atctctagaa tactcttatc ctgtaccatc ttctctctga 3600
taagcctaaa tttgtacaat tcataagcct aaaaggtgac ttatataata tatacaagga 3660
ccctcaagag ttgtttggca attcagtgac tgtcctgggt cctgttttgg ggagcttctg 3720
gtagcttttg cttctccaaa agaaaagcta gaagctcccc ccaaacagag cagcttcttc 3780
aagccggtaa aagcttcaaa agctataatt atactaaaaa cagtgaagct ccctcagagc 3840
agcttcccag ctctccagga gatgcttttg gagaagctac agtttcccca aacagggccc 3900
tgctctgttg aacccccctt cctgatacat atttgaatat gagtttatag tgtgtgtggg 3960
ggggtgtaag taggggggta atgggttcta aattttatac tataaaaatt aaggatcaga 4020
ttagaattga gctctatttc tattcatttt tgaactaaaa ttaattaagg gctcaaatga 4080
attatgaaga agcattagga tcatgatcca ttaccacccc tacgtgtaag atgttttttg 4140
gtggttgtgg ttgattttga attttaaggc cgcatatgtc tcatggacta cacaagctca 4200
tattcatcta catttgtagc cgtcactaac ttagccaaat atgcatatgt ggcggctgag 4260
agcacctaga gggggggggg gtgaataggt gatcctataa aaacttgaaa cataatgcca 4320
caaaacttga ttaggagtta gcacaataaa gccaagtgac tagagaggag ttcttgcaag 4380
acacgataac cacacgaaga tcaacacaga tagacacaat ggtttatccc gttgttcggc 4440
caagtccaac acttgcctac tccacgttgt ggcgtcccaa cggacgaggg ttgcaatcaa 4500
cccctctcaa gcggtccaag gacccacttg aataccacgg tgttttgctt agtttcacta 4560
tatcccgctt gcgaggaatc tacacaactt ggagcctctc gcccttacaa tttgatgttc 4620
acaaagaagc acgaaagtaa ggctgggatg agcaacgcac acaagacaca aaatcagagc 4680
acaacacgca cacaagtcac aacacgagct cacaacacaa cccaaagagt tctctactca 4740
aatggagctc tagttgctat cacaaagaat cgaatacgcg gaattggagt cttggtgctt 4800
agaaacgctt agagaatgct tggtgtgttc ctccatgcgc ctaggggtcc cttttatagc 4860
cccaaggcag ctaggagccg ttgagagcat tccaggaagg caattcttgc cttctgtcgc 4920
ctggcgcacc ggacagtccg gtgcaccacc ggacactgtc cggtgcggat ttctttcctt 4980
ctttagcgaa gccgaccgtt ggagattcag agccgttggc gcaccggaca gtgtccggtg 5040
cacaccggac agtccggtgc ccccttctga ccgttggctc tgccacgcgt cgcacgcgga 5100
ttacgcggcc gaccgttggc ccggccgact gttggctcac cggatagtcc ggtgcaccac 5160
cggacagtcc ggtgatttat agccgtacgc cgccgacgaa acccgagagc agccagttcg 5220
ccagagccag cctggcgcac cagacactgt ccggtgcacc cagactacgc agtcttggct 5280
gcacagccaa gtcttttcca aattggtctt tttctgtttc tagcacttag acacattaca 5340
ttagtatcca aaacaatgta ctaagtctta gaaacatacc tttactcttg atttgcactt 5400
tgtccatcat ttggcataga ttaacacatg accacttgtg ttggcactca atctccaaaa 5460
tacttagaaa tggcccaatg gcacatttcc ctttcaatct cccccttttt ggtgatttat 5520
gccaacacaa caaaaagcaa ctaaaagaag tgcaacatca atgcaaatga gaacaaaaaa 5580
ttgttttgat tcaaatttgg catatttgga tcattctttg ccaccacttg gttttgtttt 5640
tgcaaatcaa cctcaatttc ctatctctaa gtcaaacaca cttgttgaaa cataaagaga 5700
gttgttccat gagaaattga tcaaagattt caaaaactcc cccttttttc cataatcaaa 5760
cattctcccc acaagagacc aacttttgac agaagagaca ataagagaat tttgacaaac 5820
caaaaagctc tattctacta ttttcaaaat tctcaagtgg tagctgatcc atttattgct 5880
ttggccttat tttctccccc tttggcatca agcaccagaa cgggataaat cttggccctt 5940
aaaaccccat tgcctcacca aaatcttcaa ttaagagtaa aaaggcaata agagcatgaa 6000
gatgaacttg gagttagtta ctctttcatc ggagtgcagt ggaagtcttt catggtccaa 6060
gtccaacatt tcctttcaat ccacctttga gactaaatca agcaaactca agcacacagt 6120
tagtctcaag gggtcaagtt gtagcacaac tccccctaaa tatgtgcatt acttgcaaat 6180
ggacttgtga ggtccgggga gtgtttgtac aacttgagca ccatacataa acaacaaaat 6240
gcataaagga acatgatcaa ggcataaaac acatgtatgc tataaatcaa tccaagttcc 6300
gcgaatctaa gacatttagc tcactacgca gcctacaaaa ggtcttctca tctagaggct 6360
tggtaaagat atcggctagc tggttctcgg tgctaacatg aaacacttcg atatctccct 6420
tttgctggtg atctctcaaa aagtgatgcc ggatgtctat gtgcttagtg cggctgtgtt 6480
caacaggatt atccgccatg cggatggcac tctcattatc acataggagt gggactttgc 6540
tcagattgta gccaaagtcc ttgagggttt gcctcatcca aagtagttgc gcgcaacact 6600
gtcctgcggt aacgtactcg gcctcagcgg tggatagggc aacggaggtt tgtttcttag 6660
aattccacga caccagggac cttcctaaga attgacacgt ccccgatgta ctctttctat 6720
cgaccttaca tccaacatag tcggagtctg aatatccaat caagtcaaag gtagacccct 6780
ttggatacca gatcccgaag caaggcgtag cgactaaata tctaagaatt cgcttcacaa 6840
ccactaagtg acactccttt ggatcggatt gaaatctagc acacatgcat acacttagca 6900
taatatctgg tctactagca cataaataaa gtaaggaccc tatcatagac cggtatgcct 6960
tttgatcaac ggacttacct cctttgttga ggtcggtgtg tccgtcagtt cccattgtag 7020
tctttgcggg cttggcgttc ttcatcccaa actgctttag cagatcttgc gtgtacttcg 7080
tttgggagat gaaagtgccg tccttgagtt gcttcacttg gaacccaagg aagtagttca 7140
actcgcccat catcgacatc tcgaatttct gagtcatcac cctgctaaac tcttcacaag 7200
acttttggtt agtagaacca aatattatgt catcgacata aatttggcac acaaaaagat 7260
caccatcaca agtcttagtg aataaagttg gatcggcttt cccaaccttg aaagcattag 7320
caagtaaaaa gtctctaagg cattcatacc atgctcttgg ggcttgctta agtccataga 7380
gggccttaga gagcttacac acgtggtcgg ggtaccgttc atcctcgaag ccagggggtt 7440
gctccacgtg cacctcctcc ttgattagcc cgttgaggaa agcactcttc acatccattt 7500
ggaacaacct gaaggaatgg tgagcggcat aggctaacaa aatacgaata gactctagcc 7560
tagccacagg agcaaaagtc tcctcaaagt ccaaacctgc gacttgggca tagccttttg 7620
ccacaagtct cgccttgttc cttgtcacca ccctgtgctc gtcctgtttg ttgcggaaca 7680
cccacttggt tcccacaacg ttttgcttgg gacgaggcac cagtgtccaa acttcatttc 7740
gcttgaagtt gatgagctct tcctgcatgg ccaacaccca gtccggatct agcaaggcct 7800
cttctaccct gaaaggctca atagaagaga caaaagagta atgctcacaa aatttaacta 7860
atctagagcg agtagttact cccttgctaa tgtcacccaa aatctggtcg acgggatgat 7920
tcctttgaat cgtcgctcga acttgagttg aaggggcttg aggtgcttct tcctccataa 7980
catgatcatc ttgtgctccc ccttgatcac atgcctcctc ttgatgaacc tgttcatcgt 8040
cttgagttgg gggatgtacc aatgttgagg aagaaggttg atcttgctcc ttttgttcct 8100
gtggccgcac atctccaatc gtcatggtgc gtattgcggc cgttggaatg tcttcttcat 8160
ctacatcatt aagatcaaca acttgctctc ttggagagcc attagtctca tcaaatacaa 8220
cgtcgctaga gacttcaacc aaactcgatg atttgttgaa aaccctatac gcctttgtat 8280
ttgagtcata acctaacaaa aacccttcta cagctttggg agcaaactta gaatttctac 8340
ctttcttcac tagaatgtag catttactcc caaatacacg aaagtatgaa acattgggtt 8400
tgttaccggt taggagctca tacgaagtct tcttgaggag gtgatgaagg tagacccggt 8460
ttatggcgtg gcaagccgtg ttcacggctt ccgaccaaaa tcgctcgggg gtcttgaact 8520
ctccaagcat cgtcctcgcc atgtcaatga gcgtcctatt tttcctctct accacaccat 8580
tttgctgtgg tgtgtaggga gcggagaact cgtgcttgat tccttcctcc tcaaggtact 8640
cttctacttg aagattcttg aactccgacc cgttgtcgct ccttatcttc ttcaccttga 8700
gctcaaactc attttgagct ctccttagga agcgcttgag ggtcccttgg gtttcagatt 8760
tatcctgcaa aaagaatacc caagtgaagc gggaaaaatc atcaactata acaagaccat 8820
acttacttcc tcctaagctt aggtaggcga cgggcctgaa gaggtccata tgtagcaact 8880
ccaaaggtct tgatgtggtc atcacatttt tggtatgatg agagcttccc acttgtttac 8940
ctgcttgaca agctgcataa ggtctatctt tttcgaaagt aacatttgtt agtcctatca 9000
cgtgttctcc ctttagaagc ttgtgaaggt tcttcatccc cacatgtgct aagcggcgat 9060
gccacagcca gcccatgcta gtcttagcaa ttaagcatgc atctagaccg gcctcctctt 9120
ttgcaaaatc aactaagtag agtttgccgt ctaatacacc cttaaaagct aatgaaccat 9180
cactccttct aaagacggac acatctacat ttgtgaataa gcaattatat cccatattac 9240
aaagctaact cacagacaat aagttatatc caagagactc aactaaaaac acattagaaa 9300
tagagtgctc ggatgaaatg gcaatcttcc ctagtccttt gaccttgcct tgattcccgt 9360
caccgaatat gattgagtct tgggaatcct tgttcttgac gtaggaggtg aacatcttct 9420
tctcccccgt catgtggttt gtgcatccgc tgtcgataat ccagcttgat cccccggatg 9480
cataaacctg caaggcaaat taggcttggg tcttaggtac ccaactcttg ttgggtccta 9540
caaggttagt acaaatagcc ttagggaccc aaatgcaagt tttatctccc ttgcattttg 9600
cccctaattt tctagcaacc accttcttat cctttctaca aatatcaaag gaagcattta 9660
aagcatgata aattgtagaa ggttcattac ttgttttcct aggtacatga gcatttctcc 9720
taggcacatg atgaatgata tttttcctag ccaaatttct atcatgcata atagaagaac 9780
ttgaagcaaa cattgcattt gaatcataag catgtgaaat gacatcattg caacttctat 9840
catgatgaac attcctggaa tatctcctat catggtataa gaaagcatgg ttcttttgaa 9900
tactatttgc cataggggcc ttccctttct ccttgatgga gataggagcc ttatgacttg 9960
tcaagttctt ggcttccctc ttgaagccaa gcccatcctt aattgagggg tgtctaccaa 10020
ccgtgtaggc atcccttgca aattttagtt tatcaaaatc atttttgcta gtcttaagtt 10080
gagcattaag actagccact tcatctttta gtttagaaat gcaaactagg tgttcactac 10140
aagcatcaac attgaaatct ttacacctat tgcaaatcgt aacatgttct tcacgagagg 10200
ttaatttact agctatttct aacttagcac tcaaatcatc attaacactt tttaggctag 10260
agatagattc atggcatgta gacaattcac atgaaagcat ttcatttctt ttaatttcta 10320
aagcaagaga attttgtgct tctacaaact tatcatgttc ttcatacaaa agatcctctt 10380
gcttttctaa taatctattc ttatcattca aggcatcaat caactcattg atcttatcaa 10440
tcttagttct atctaagccc ttgaacaaac tagcatagtc tatttcatca tcgctagatt 10500
catcatcact agaagcataa gtagactttc gagtgtttac cttcttctcc tttgccatta 10560
agcatgtgtg atgctcgttg ggggaagagg aacgacttgt tgaaggccga ggcgacgagt 10620
ccttcgttgt cggagtcgga cgacgaacaa tccgagtccc actccttgcc aaggtgtgcc 10680
tcacccttag ccttcttata agccttcttc ttttccctct tcttctcttg ttcctggtca 10740
ctatcattat cgggacaatt agcgataaat gaccaatctt accacatttg aagcatgagc 10800
gcttcccctt cgtcttgttg ggatgctcct tgcgaccctt tagtgctgtc ttgaatcgct 10860
tgatgatgag ggccatttct tcttcattaa gcccgaccgc ctcaacttgc gccaccttgc 10920
taggtagcgc ctccttgctc ctcgttgctt tgagagccac agtttgaggc tcgtggattg 10980
ggccattcaa cgcatcatcg acgtatcttg cctccttgat catcatccgc ccgcttacga 11040
actttccaag tatttcttcg ggcgacatct tggtgtacct aggattttca cgaatattgt 11100
ttacaagatg tggatcaagg atagtgaagg accttagcat taggcggacg acgtcgtgat 11160
ccgtccatcg cgtgcttcca tagcttctta ttttgttgac gagggtcttg agccggttgt 11220
acgtttgggt tggctcttct cccctgatca ttgcgaatct cccaagttcg ccctccacca 11280
actccatctt ggtgagcatg gtgacatcgt tcccctcatg tgagatcttg agggtgtccc 11340
agatctgctt ggcgttatcc aagccgctca ccttatggta ttcatccctg cacaatgatg 11400
ctagaagaac agtagtagct tgtgcatttt tgtgaatttg ctcattgata aatatgggac 11460
tatcagtact atcaaattgc attccactct ctactatctc ccatatgctt ggatggagag 11520
agaacaagtg actacgcatt ttgtgactcc aaaatccgta gtcctctcca tcaaagtgag 11580
gaggtttacc aagtggaatg gagagtaaat gagcatttgt actttgcgga atacgagaat 11640
aatcaaaaga aaagtttgaa ttgactgttt tctttttctc gtagttgtcg tcgtcctttt 11700
gggaagaaga ggactcgtcg ctgtcgtcgt agtagacgat ctccttgatg caccttgttt 11760
tcttcttctt cctgtctttt cttttgtggc tcgagcccga gtcagtaggc ttgtcatctt 11820
ttggatcatt gacgaaggac tccttctcct tatcattgac caccatcccc ttgcccttag 11880
gatccatctc ttcgggcgat tagtccctta cgtgaagaga acgactcaga taccaattga 11940
gagcacctag aggggggtga ataggtgatc ctataaaaac ttgaaactta atgccacaaa 12000
acttgattag gagttagcac aataaagcca agtgactaga gagttcttgc aagacacgat 12060
aaccacacaa agatcaacac agatagacac agtggtttat ctcgtggttc ggccaagtcc 12120
aacacttgcc tactccacgt tgtggcgtcc caacggacga gggttgcaat caacccctct 12180
caagcggtcc aaggacccac ttgaatacca cagtgttttg cttagtttca ctatatcccg 12240
cttgcgagga atctccacaa cttgtagcct ctcgccctta caatttgatg ttcacaaaga 12300
agcacgaaag taaggctggg atgagcaacg cacacaagac acaaaatcag agcacaacac 12360
gcacacaagt cacaactcga gctcacaaca caacccaaag agttctctac tcaaatggag 12420
ctctagttgc tatcacaaag aatcgaatgc gcggaattgg agtcttggtg cttaggaacg 12480
cttagagaat gctttgtgtg ttcctccata cgcctagggg tcccttttat agccccaagg 12540
cagctaggag ccgttgagag cattccagga aggcaattct tgccttctgt cgcctggcgc 12600
accggacagt ccggtgcacc atcggacact gtccggtgca gatttctttc cttttttagc 12660
gaagccgacc gtcggagatt cagagccgtt ggcgcactgg acactgtccg atggacaccg 12720
gacagtccgg tgcccccttc tgaccgttgg ctctgccacg cgtcgcgcgc ggattacgcg 12780
gccgaccgtt ggctcgaccg actgttggct cactgaacag tccggtgcac caccggacag 12840
tccggtgatt tatagccgta cgccgccgac gaaacccgag agcagctagt tcgctagagc 12900
cagtctggcg caccagacat tgtccggtgc accaccggac agttcggtgc acccagactg 12960
cgcagagtct tggctgcaca gccaagtctt ttccaaattg gtcttttcct gtttctagca 13020
cttagacaca ttacattagt ctccaaaaca atgtactaag tattagaaac atacctttac 13080
tcttgatttg cactttgtcc atcatttggc atagattaac acatgaccac ttgtgttggc 13140
actcaatctc caaaatactt agaaatggcc caagggcaca tttccctttc agcggctagc 13200
aacaggtcct tggtttcttg ggttatttat tctcttttta tcgtgtttga atgttttcgt 13260
gttcatttgc ataacatctt aggtctacat cagtatatga attgagatca aatgtgaatt 13320
ggaccacaca agctcatatt catctacatt tgtagtcgtc actaacttag ccaaatatgc 13380
atatgtccgc ttctgatttc attgtgtctt ttcttcagga gtttggggat caaggagagg 13440
actccattat cttgtcaccg cgactgaagg agattagtac tcctgaccgc cccgctgccc 13500
tccgtttcct aggtac 13516
<210> 56
<211> 1026
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA Snare T1
<400> 56
gcgcccggct cttcatcgcc tccacccgct ccagcgtctc caccacctcc ttcatcgagg 60
gccgactgcg aggctcgccg gccaggcagc cgagcgtcag ttgcgccgct tggaacgcct 120
gcttttgttg atcgtttgtt ttggtctgat ttcggtgggt ctatccgcag agaggaagaa 180
gcagaagctc tccgagatcc aatccggcgt tgaggaagct gaatcgctga ttcagaaaat 240
ggacctggag gcaaggagcc tacagcctag cattaaggct agtttgcttg caaagctgag 300
ggagtataaa tctgacctca acaacgtcaa gagtgagctc aagaggatat ctgcgcccaa 360
tgccaggcag gctacccggg aggagctcct ggagtctgga atggctgata ctctcgcagt 420
gagctaatga taggacttga ctgtgtctac gagactgctc ctaacaataa actgaagaaa 480
gcaaaagaaa tcattcaacg tattcgccga agagaactct acaagatatg gtagtcctgg 540
gaggaagagc catcgccgct atgtatggca gaaccacccg cgaaagcaac ctctctggtc 600
tcgcatagcc agagcaggag cagctcgctt gcgcggccgc agcgctggcg gtcggccccg 660
cgtacgagcg cctgcaggaa gccaggaacc catccgaaca aggttgcaat catgataagc 720
agatagagca agcatatgat gatattttga attcgtcgaa gcatactttg gccagcatga 780
tggagctgca ggaggctttg cttgagagta atcaggctac aaaggatgcc aatgaaattc 840
cttctgcctc aaatggagac aatgacgagt ggtcagaagt acagagattg cagacaaggt 900
aaattttgca atagaaataa ctaaccaacc attagtgctt gaaaaaaact ggactggtga 960
ctggggcacg tggtttcatc aacatttgga cctcaacggt ctaatcagta taacttagaa 1020
gttggc 1026
<210> 57
<211> 874
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA Snare T1
<400> 57
gcgcccggct cttcatcgcc tccacccgct ccagcgtctc caccacctcc ttcatcgagg 60
gccgactgcg aggctcgccg gccaggcagc cgagcgtcag ttgcgccgct tggaacgcct 120
gcttttgttg atcgtttgtt ttggtctgat ttcggtgggt ctatccgcag agaggaagaa 180
gcagaagctc tccgagatcc aatccggcgt tgaggaagct gaatcgctga ttcagaaaat 240
ggacctggag gcaaggagcc tacagcctag cattaaggct agtttgcttg caaagctgag 300
ggagtataaa tctgacctca acaacgtcaa gagtgagctc aagaggatat ctgcgcccaa 360
tgccaggcag gctacccggg aggagctcct ggagtctgga atggctgata ctctcgcagt 420
gagctaatga taggacttga ctgtgtctac gagactgctc ctaacaataa actgaagaaa 480
gcaaaagaaa tcattcaacg tattcgccga agagaactct acaaggaagc caggaaccca 540
tccgaacaag gttgcaatca tgataagcag atagagcaag catatgatga tattttgaat 600
tcgtcgaagc atactttggc cagcatgatg gagctgcagg aggctttgct tgagagtaat 660
caggctacaa aggatgccaa tgaaattcct tctgcctcaa atggagacaa tgacgagtgg 720
tcagaagtac agagattgca gacaaggtaa attttgcaat agaaataact aaccaaccat 780
tagtgcttga aaaaaactgg actggtgact ggggcacgtg gtttcatcaa catttggacc 840
tcaacggtct aatcagtata acttagaagt tggc 874
<210> 58
<211> 553
<212> DNA
<213> Zea mays
<400> 58
cgatgtgcag tggcctgatt agctacaaga agctcttgtt ccatggactc gatctctgga 60
ccgcactatc gttgcctcag cccctaggtc atgctgccct ctggcctcct catcgtacaa 120
ttcaccaaca tctccaatgt aagtgcagct ggttcagtaa tgaactcaga agtggcatca 180
gaatactcca agagtttttt gttctttttg cctggatata taccaaggga aatgcattca 240
aaactcctat agatgacgaa tcccatctct ccctcttttc tcggacacgg atccccaggt 300
ccgtctccgt gctttactca tttgtttttt acaagttcag atccacttgc gtactcacac 360
agtggacatc tgttatgcac atgtgtaaac cagcataaga attaggaatt atgctcattt 420
tatctaagaa gtccttacac tcgaaaatgc atgtgttatt tagcttgaga ataaataaaa 480
ttattagcaa ggagaaaaaa aataggacta aagaatagag tcacattggt ttaaattagt 540
acctagaagc aaa 553
<210> 59
<211> 527
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA Snare T2
<400> 59
gcttctcgat gtgcagtggc ctgattagct acaagaagct cttgttccat ggactcgatc 60
tctggaccgc actatcgttg cctcagcccc taggtcatgc tgccctctgg cctcctcatc 120
gtacaattca ccaacatctc caatgtaagt gcagctggtt cagtaatgaa ctcagaagtg 180
gcatcagaat actccaagag ttttttgttc tttttgcctg gatatatacc aagggaaatg 240
cattcaaaac tcctatagat gacgaatccc atctctccct cttttctcgg acacggatcc 300
ccaggtccgt ctccgtgctt tactcatttg ttttttacaa gttcagatcc acttgcgtac 360
tcacacagtg gacatctgtt atgcacatgt gtaaaccagc ataagaatta ggaattatgc 420
tcattttatc taagaagtcc ttacactcga aaatgcatgt gttatttagc ttgagaataa 480
ataaaattat tagcaaggag aaaaaaaata ggactaaaga atagagt 527
<210> 60
<211> 9062
<212> DNA
<213> Zea mays
<400> 60
gttgcgagat gtttatgtgc cttagtcttc aatttggggg ttgggggaaa agtaatttta 60
tgtttttgtt ttgtgtctgc agattcggaa gatggacttg gaggcaagga gcctacagcc 120
tagcattaag gctggtttgc ttgcaaagct gagggagtat aaatctgacc tcaacaacgt 180
caagagtgag ctcaagagga tatttgcgcc caatgccagg caggctaccc gggaggagct 240
cctagagttt ggaatggctg atactctcgc tgtgagctaa tgctaggact tgactgtgtc 300
tacgagactg ctcctaacaa taaactgaag aaagcaaaag aaatcattca acgtattcgc 360
cgaagagaac tctacaaggt agtatgatgc tttaattgct catatacaag tgtcattttg 420
tcatgtcatt acacatggtt aggatacata cttaagtttc taacgtaggc gtccacacaa 480
cggattggtg cacggttctg ccgatgtatc ccacgcacgt gcatggaagg aggcaggcac 540
ccttccccgc cgccccggat ctcgcgccag cccccgccct accccgcctg cccttccact 600
cttcccccgc tgcccccggt caacgtcacg aacccgggcc tcgtgccgct cgtcgtggcc 660
acactgttcg acgagcgagt cacagagctg ctgagcgtgc tcgctgatgc ggcggtgggg 720
cgaccaggca ggtggtccat cggcgaagcg ccatggtcgt cgtcgggggg cacgaaccag 780
gcggtgtacg cgcgccgcgc gcccggctct tcatcgcctc cacccgctcc agcgtctcca 840
ccacttcctt catcgagggc cgactgcttg gctcgctggc caggcagccg agcattagtt 900
gcgccgcttg gaacgcctgc ttttgttgat cgtttgtttt ggtctgattt cagtgggtct 960
atccgcagag aggaagaagc agaagctctc cgagatccaa tccggcgttg aggaagctga 1020
atcgctggta aatagatgtc gcgacgcgtt ctgttttggg gatccccttg gctaacggga 1080
catacgacat ttggggaatg ggtagaaaag cagagattag ggatttttcg tttccgtcgg 1140
tgcagttttg gtgttccaac agagttgcga gatgtttatg tgccttagtc ttcaatttgg 1200
gggttggggg aaaagtaatt ttatgttttt gttttgtgtc tgcagattca gaaaatggac 1260
ctggaggcaa ggagcctaca gcctagcatt aaggctggtt tgcttgcaaa gccgagggat 1320
tataaatctg acctcaacaa cgtcaagagt gagctcaaga ggatatctgc gcccaatgcc 1380
aggtaggcta cccgggagga gctcgtggag tctagaatgg ctgatactct cgcagtgagc 1440
taatgctagg acttgactgt gtctacgaga ctgctcctaa taataaactg aagaaagcaa 1500
aagaaatcat tcaacgtatt cgccgaagag aactctacaa ggtagtatga tgctttaatt 1560
gctcatatac aagtgtcatt ttgtcatgtc attacacatg gttaggatac atacttaagt 1620
ttctaacgta ggcatccaca caatggattg gtgcacggtt ctgccgatgt atcccacgca 1680
cgcgcatgga aggaggcagg cacccttccc tgccgccccg gatctcgcgc cagccatcgc 1740
cctaccccgc ctgcccttcc actcttcccc ctgaaagtcg cctagagggg gggtgaatag 1800
ggcgaatctg aaatttacaa acttaagcac aactacaagc cgggttaacg ttagaaatat 1860
aaacgagtcc gagagagagg gcgcaaaaca aatcatgagc aaataaagag tgagacacga 1920
tgatttgttt taccgaggtt cggttcttgc aaacctactc cccgttgagg tggtcacaaa 1980
gaccgggtct ctttcaaccc tttccctctc tcaaacggtc acttagaccg agtgagcttc 2040
tcttctcaat caaacgaaac acaaagttcc cgcaaggacc accacacaat tggtgtctct 2100
tgccttggtt acaattgagt ttgatcacaa gaagaatgag aaagaaaaga agcgatccaa 2160
gcgcaagagc tcaaatgaac acaaatgtcg ctctctctag tcactatttg atttggagtg 2220
attccggact tgggagagga tttgatcttt tggagtgtct agaattgaat gctatagctc 2280
ttgtaatatg ttgaaggtgg gaaacttgga tgccattgaa tgtggggtgg ttggggtatt 2340
tatagcccca aaacaccaaa aaaggccgtt ggaaggctgc tctcgcatgg cgcaccggac 2400
agtccggtgc gccagccacg tcagcagacc gttggggttc gaccgttgga gctctgactt 2460
gtggggcctc tgggctgtcc ggtggtgcac cggacaggtc ctgtaggatg tctggtgcgc 2520
caactgcacg tgctctgtcc tctgcgcgcg caggcgcgca ttaaatgcgt tgtagtcaac 2580
cgttgcgcgc gaagtagcca ttgctctgct ggcacaccgg acagtccggt gaattatagc 2640
ggagcgccct ctgattttcc cgaaggtagc gagttcagct tcgagtgccc tggtgcaccg 2700
gacactgtcc ggtgcgccaa accagggtgc cttccgggat gtcttttgct ctctttgttt 2760
gaaccctttc ttggtctttt tattggctta ttgtgaacct ttgacacctg taaaacttat 2820
agactagagc aaactagtta gtccaattat ttgtgttgga caattcaacc accaaaatca 2880
attaggaaat aggtgtgagc ctaattccct ttcaatctcc ccctttttgg tgattgatgc 2940
caacacaaac caaagcaagt atagaagtgc ataattgaac tagtttgcat aatgtaagtg 3000
caaaggttac ttagaattga accaataaat attttcataa gttatgcatg gattgtttct 3060
ttattttcat cattttggac cacgcttgca ccacatgttt tgtttttgca aatccttttg 3120
taaatagtca aaggtaaatg aataagattt tgagaagcat tttcaaaatt tgaaattttc 3180
tccccctgtt tcaaatgctt ttcctttgac ttaaacaaaa ctcccccctc aaaaatccta 3240
ctcatagtgt tcaagagggt tttaagatat caattttgaa aatgctactt tctccccctt 3300
ttgaatataa taagatatca attgaaaaat tcatcatttt aaaacctttt gaaaatgggt 3360
ggtggtgcgg tccttttgct ttgggctaat actttctccc cctttggcat gaatcgccaa 3420
aaacgaatac ttgagtgaaa tataagcccc tttaactact ttctcctgct ttggcgaaca 3480
taatatgagt gaagattata ccaaagttgg agagttgctt gaagcgacgg tgaaggatga 3540
gttatggagt ggaggttaag cctttgtctt cgccgaagat tccaattccc tttcaataca 3600
cctatgactt ggtttgaaat atacttgaaa acacattagt catagcacat gaaagagata 3660
tgatcaaagg tatattaatg agctatgtat gcaagacatc aaaagaaatt cctagaatca 3720
agaatattta gctcgtgtct aagtttgttc atctagtggc ttggtaaaga tatcggctaa 3780
ttgttcctta gtgttaatat aggcaatctc gatatctccc tttttttggt gatcccttag 3840
gaaatgatac cgaatggcta tgtgtttagt gcggctatgc tcaacgggat tatccgccat 3900
gcggattgca ctctcattat cacatagaag aggaactttg gttaattttt aaccatagtc 3960
cctaagggtt tgcctcatcc aaagtaattg tgcgcaacaa tggcctgcgg caatatactc 4020
ggcttcggcg gtagaaagag ctacggaatt ttgcttcttt gaagcccaag acaccaggga 4080
ccttcccaag aactggcaag tccccgatgt actctttcta ttaattttac accccgccca 4140
atcggcatcc gaataaccaa tcaaatcaaa tgtggatccc gtaggatacc aaagcccaaa 4200
cttaggagta tgaactaaat atctcaagat tcgttttacg gccgtaaggt gagcttcctt 4260
agggtcggct tggaatcttg cacacatgca tacggaaagc ataatatccg gtcgagatgc 4320
acataaatag agtaaagagc ctatcatcga ccggtatacc ttttgatcga cggatttacc 4380
tcccgtgtcg aggtcgagat gcccattggt tcccatgggt gtcttgatgg gtttggcatc 4440
cttcatccca tacttgttta gaatgtcttg aatgtacttc gtttggctaa tgaaggtgcc 4500
ctcttagcgt tgcttcactt gaaatcacaa gaagtacttc aactccccca tcatagacat 4560
ctcgaatttc tgtgtcatga tcctactaaa ttcctcacat gtagattcat tagtagaccc 4620
aaatataata tcatcaacat aaatttggca tacaaacaaa tcattgtcaa gagttttagt 4680
aaataaagta ggatcggcct ttccgacttt gaaaccatta gtgataagga aatctctaag 4740
gcattcatac catgctcttg gggcttgctt gagcccataa agcgccttag agagtttata 4800
tacgtgatta gggtactcac tatcttcaaa gccgggaggt tgctcaacat agacctcttc 4860
cttgattggt ccgttgagga aggcactttt cacgtccatt tgataaagct tgaagccatg 4920
gtaagtagca taggcaagta atatgcgaat tgactcaagc ctagctacgg gtgcataggt 4980
ttcaccaaaa tccaaacctt cgacttgtga ataacccttg gccacaagtc gggctttgtt 5040
ccttgtcacc acaccatgct catcttgttt gttgcggaag acccacttgg ttcctacaac 5100
attttgatta ggacgtggaa ctaagtgaca tacctcattc ctagtgaagt tgttgagctc 5160
ctcttgcatc gccaccaccc aatctgaatc ctgtagtgct tcctctaccc tttgtggctc 5220
aatagaggaa acaaaagagt aatgttcaca aaaatgagca acacgagatc gagtagttac 5280
ccccttttga atatcgccga ggatggtgtt cacggggtga tctcgttgga ttgcttggtg 5340
gactcttggg tgtggcggtc ttggttcttc atcctccttg tcttgatcat ttgcatctcc 5400
cccttgatta ttgccgtcat cttgaggtgg ctcatcttct tgatcttctc ctttatcatc 5460
ttgagcctca tcctcatttt gagttggtgg agatgcttgc gtggaggagg atggttgatc 5520
ttgtgcattt ggaggctctt tggattcctt aggacacaca tccccaatgg acatgttcct 5580
tagcgcgacg cacggagcct cttcatcacc tatctcatca agatcaactt gctctacttg 5640
agagccgtta gtttcatcaa acacaatgtc accagaaact tcaactagtc ccgaggactt 5700
gttaaagact ctatatgccc ttgtgtttga atcataccct agtaaaaagc cttctacagc 5760
cttaggagca aatttagatt ttctacctct tttaacaaga atgaagcatt tgctaccaaa 5820
gactctaaaa tatgaaacat tgggctttta ccggttagga gttcatatga tgtcttcttg 5880
aggattcggt gtagatataa ccggttgatg gtgtagcaag cggtgttgac cgcctcgatc 5940
caaaaccgat ccgaagtctt gtactcatca agcatggttc ttgccatgtc caatagagtt 6000
cgattcttcc tctccactac accattttgt tgtggggtgt agggagaaga gaactcatgc 6060
ttgatgccct cctcctcaag gaagccttcg atttgagaat tcttgaactc cgtcccgttg 6120
tcgcttctaa tctttttgat tcttaagccg aactcatttt gagcccatct caagaatccc 6180
tttaaggtct cttgggtatg agatttttcc tgcaaaaaga atacccaagt gaagcgagta 6240
taatcatcca caataactag atagtactta ctcccgccga tgcttatgta agctatcggg 6300
ccgaataggt ccatatgtag gagctcgagt ggcctgtcag tcatcatgat gttcttgtgt 6360
ggatgatgag taccaacttg cttccctgtc tgacatgcgc tacaaatcct atctttctca 6420
aagtgaacat ttgttagtcc caaaatgtgt tctcccttta gaagcttgtg aagattcttc 6480
attccaacat gtgctagtcg gtgatgccag agccagccca tgttagtctt agcaattaag 6540
caagtgtcga gttcagctct atcaaaatct accaagtata gctgaccctc taacactccc 6600
ttaaatgcta ctgaatcatc acttcttcta aagacagtaa cacctatatc tgtaaaaaga 6660
cagttgtagc ccattttaca taattgcgaa actgaaagca agttgtaatc taaagaatct 6720
acaagaaaca cattggaaat ggaatggtca ggagatatag caattttacc caatcctttg 6780
accaaacctt ggtttccatc cccgaatgtg atcgctcttt ggggatcttg gtttttctca 6840
taggaggaga acattttctt ctcccctgtc atatggtttg tgcacccgct atcgatgatc 6900
caacttgggc ccccggatgc ataaacctac aaaacaagtt tagttcttga ttttaggtac 6960
ccaaatggtt ttgggtcctt tgacattaga tacaagaact ttgggtaccc aaacacaagt 7020
ctttgatccc ttgtgtttgc ccccaacata cttggcaact atcttgtcgg atttgttagt 7080
taaaacataa gatgcatcaa aagttttgaa tgaaatgtta tgatcatttg atgcagcagg 7140
agttttcttc ttaggcaatt ttgcacgggt tgattgccta gagctagatg tctcaccctt 7200
atacataaaa gcatgattat ggccagagtg agacttccta gaatgaattc tcctaatttt 7260
gctctcggga taaccggcag ggtacaaaat gtaaccctca ttatcctgag gcatgggagc 7320
cttgccctta acaaagtttg acaatctttt aggagaggca ttaagtttga cattgtttcc 7380
cttttggaag ccaatgccat ccttgatgcc agggcgtctc ccactataga gcatgcttct 7440
agcaaattta aatttttcat tttttaagtc atgctcggca attttagcat ctaattttgc 7500
tatatgatta ttttgttgtt taattaaagc catatgatca tgaatagcat caatgttaat 7560
atctctacat ctagtgcaaa taatgacatg ctcaatggca gatgtagagg gtttgcaaga 7620
attaagttca acaatcttag cacgtaaaat atcattgtta tttctaagat cagaaatgga 7680
agcattgcaa acatctaatt ctttagcctt agcaatcaat ttttcatttt caaccctaag 7740
gctagcaaga gagacattca attcttcaat cttagcaagc aaattaacat tatcatctct 7800
aagattggga attgaaacat cacaaatatt agaatcaacc ttagcaatta gtttagtatt 7860
tttatttcta aggatggtaa tagtatcatg gcaagtgctt agctcactag ataatttttc 7920
acatttttct acttctagag cataagcatt tttaacctta acatgcttct tattttcctt 7980
aattaggaag tcctcttgaa agtccaagag atcatctttc tcatgaatag cactaattaa 8040
ttcatttagt ttttcctgta gttgcatgtt taggttggca aaaagggtac gcaaattatc 8100
ctcctcatca ctagcattat cttcatcact agaggatgca tatttagtgg aggattttga 8160
ttttaccttc ttctttttgc cgtcctttgc catgaggcac ttgtggccga cgttggggaa 8220
gaggagccct ttggtgacgg cgatgttggc ggcgtcctcg tcggatgagg agtcggagga 8280
actctcgtcg gagtcccact cgcggcacac atgggcatcg ccgcccttct tcttgtaata 8340
cctcctcttt tctctcctct tgcccttctt gtcgtcgccc ctgtcactat cactagataa 8400
aggacattta acaatgaaat gaccgggctt accacacttg tagcacaccc ttttggaaca 8460
aggcttgtaa tctttcccct tcctttgttt gaggatttgg tggaagctct tgatgatgag 8520
cgccattttc tagttgtcga gcttagaggc gtcgatgggt tgtctacttg atgtagactc 8580
ctctttcttc tcctctgtcg ccttgaatgc gaccggttgt gcttcgggcg tggagggacc 8640
gtcgtgctcg ataattttct ttgagccttt gatcatcaac tcaaagctca caaagtttcc 8700
tattacttcc tcgggagtca ttagtgtata tctaggatta ccacgaatta attgaacttg 8760
cgtagggtta aggaacacaa gtgatctaag aataacctta accatttcat ggtcatccca 8820
ttttttgctc ccgaggttgc gcacttggtt caccaaggtc ttgagccggt tgtacatatc 8880
ttgtggctcc tccccttggc gaagccggaa gcgaccgagc tccccctcga tcgtctcccg 8940
cttggtgatc ttggttacct cgtctccttc gtgcgcggtc tttagcacgt cccagatatc 9000
ctttgcactc ttcaaccctt gcaccttatt atactcctct cgacttggat ttgaaatgtt 9060
gg 9062
<210> 61
<211> 1082
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA Snare T3
<400> 61
cctgcccttc cactcttccc ccgctgcccc cggtcaacgt cacgaacccg ggcctcgtgc 60
cgctcgtcgt ggccacactg ttcgacgagc gagtcacaga gctgctgagc gtgctcgctg 120
atgcggcggt ggggcgacca ggcaggtggt ccatcggcga agcgccatgg tcgtcgtcgg 180
ggggcacgaa ccaggcggtg tacgcgcgcc gcgcgcccgg ctcttcatcg cctccacccg 240
ctccagcgtc tccaccactt ccttcatcga gggccgactg cttggctcgc tggccaggca 300
gccgagcatt agttgcgccg cttggaacgc ctgcttttgt tgatcgtttg ttttggtctg 360
atttcagtgg gtctatccgc agagaggaag aagcagaagc tctccgagat ccaatccggc 420
gttgaggaag ctgaatcgct gattcagaaa atggacctgg aggcaaggag cctacagcct 480
agcattaagg ctggtttgct tgcaaagccg agggattata aatctgacct caacaacgtc 540
aagagtgagc tcaagaggat atctgcgccc aatgccagat tcggaagatg gacctggaag 600
caaggagcct acaacctagc attaagagtg agctcaagag gatatctgcg cccattgcca 660
ggcaggctac ccgggaggag ctcctggagt ctggaatggc tgatactctc gcagtgagct 720
aatgctagga cttgactgtg tctacgagac tgctcctaac aataaactga agaaagcaaa 780
agaaatcatt caacgtattc gccgaagaga actctacaag atatggtagt cctgggagga 840
agagccatcg ccgctatgta tggcagaacc acccgcgaaa gcaacctctc tggtctcgcg 900
tagccagagc aggagcagct cgcttgcgcg gtcgcggcgc tggcggccgg ccccgcgtac 960
gagcgcctgc aggaagccag gaacccatcc gaacaaggtt gcaatcatga taagcagata 1020
gagcaagcat atgatgatat tttgaattcg tcgaagcata ctttggccag catgatggag 1080
ct 1082
<210> 62
<211> 1154
<212> DNA
<213> Artificial Sequence
<220>
<223> cDNA Snare T3
<400> 62
cctgcccttc cactcttccc ccgctgcccc cggtcaacgt cacgaacccg ggcctcgtgc 60
cgctcgtcgt ggccacactg ttcgacgagc gagtcacaga gctgctgagc gtgctcgctg 120
atgcggcggt ggggcgacca ggcaggtggt ccatcggcga agcgccatgg tcgtcgtcgg 180
ggggcacgaa ccaggcggtg tacgcgcgcc gcgcgcccgg ctcttcatcg cctccacccg 240
ctccagcgtc tccaccactt ccttcatcga gggccgactg cttggctcgc tggccaggca 300
gccgagcatt agttgcgccg cttggaacgc ctgcttttgt tgatcgtttg ttttggtctg 360
atttcagtgg gtctatccgc agagaggaag aagcagaagc tctccgagat ccaatccggc 420
gttgaggaag ctgaatcgct gattcagaaa atggacctgg aggcaaggag cctacagcct 480
agcattaagg ctggtttgct tgcaaagccg agggattata aatctgacct caacaacgtc 540
aagagtgagc tcaagaggat atctgcgccc aatgccagac tgctcctaat aataaactga 600
agaaagcaaa agaaatcatt caacgtattc gccgaagaga actctacaag attcggaaga 660
tggacctgga agcaaggagc ctacaaccta gcattaagag tgagctcaag aggatatctg 720
cgcccattgc caggcaggct acccgggagg agctcctgga gtctggaatg gctgatactc 780
tcgcagtgag ctaatgctag gacttgactg tgtctacgag actgctccta acaataaact 840
gaagaaagca aaagaaatca ttcaacgtat tcgccgaaga gaactctaca agatatggta 900
gtcctgggag gaagagccat cgccgctatg tatggcagaa ccacccgcga aagcaacctc 960
tctggtctcg cgtagccaga gcaggagcag ctcgcttgcg cggtcgcggc gctggcggcc 1020
ggccccgcgt acgagcgcct gcaggaagcc aggaacccat ccgaacaagg ttgcaatcat 1080
gataagcaga tagagcaagc atatgatgat attttgaatt cgtcgaagca tactttggcc 1140
agcatgatgg agct 1154
<210> 63
<211> 107
<212> PRT
<213> Zea mays
<400> 63
Met Ile Gly Leu Asp Cys Val Tyr Glu Thr Ala Pro Asn Asn Lys Leu
1 5 10 15
Lys Lys Ala Lys Glu Ile Ile Gln Arg Ile Arg Arg Arg Glu Leu Tyr
20 25 30
Lys Glu Ala Arg Asn Pro Ser Glu Gln Gly Cys Asn His Asp Lys Gln
35 40 45
Ile Glu Gln Ala Tyr Asp Asp Ile Leu Asn Ser Ser Lys His Thr Leu
50 55 60
Ala Ser Met Met Glu Leu Gln Glu Ala Leu Leu Glu Ser Asn Gln Ala
65 70 75 80
Thr Lys Asp Ala Asn Glu Ile Pro Ser Ala Ser Asn Gly Asp Asn Asp
85 90 95
Glu Trp Ser Glu Val Gln Arg Leu Gln Thr Arg
100 105
<210> 64
<211> 131
<212> PRT
<213> Zea mays
<400> 64
Met Cys Ser Gly Leu Ile Ser Tyr Lys Lys Leu Leu Phe His Gly Leu
1 5 10 15
Asp Leu Trp Thr Ala Leu Ser Leu Pro Gln Pro Leu Gly His Ala Ala
20 25 30
Leu Trp Pro Pro His Arg Thr Ile His Gln His Leu Gln Cys Lys Cys
35 40 45
Ser Trp Phe Ser Asn Glu Leu Arg Ser Gly Ile Arg Ile Leu Gln Glu
50 55 60
Phe Phe Val Leu Phe Ala Trp Ile Tyr Thr Lys Gly Asn Ala Phe Lys
65 70 75 80
Thr Pro Ile Asp Asp Glu Ser His Leu Ser Leu Phe Ser Arg Thr Arg
85 90 95
Ile Pro Arg Ser Val Ser Val Leu Tyr Ser Phe Val Phe Tyr Lys Phe
100 105 110
Arg Ser Thr Cys Val Leu Thr Gln Trp Thr Ser Val Met His Met Cys
115 120 125
Lys Pro Ala
130
<210> 65
<211> 162
<212> PRT
<213> Zea mays
<400> 65
Met Glu Gly Gly Arg His Pro Ser Pro Pro Pro Arg Ile Ser Arg Gln
1 5 10 15
Pro Pro Pro Tyr Pro Ala Cys Pro Ser Ile Leu Pro Pro Leu Pro Pro
20 25 30
Val Asn Val Thr Asn Pro Gly Leu Val Pro Leu Val Val Ala Thr Leu
35 40 45
Phe Asp Glu Arg Val Thr Glu Leu Leu Ser Val Leu Ala Asp Ala Ala
50 55 60
Val Gly Arg Pro Gly Arg Trp Ser Ile Gly Glu Ala Pro Trp Ser Ser
65 70 75 80
Ser Gly Gly Thr Asn Gln Ala Val Tyr Ala Arg Arg Ala Pro Gly Ser
85 90 95
Ser Ser Pro Pro Pro Ala Pro Ala Ser Pro Pro Leu Pro Ser Ser Arg
100 105 110
Ala Asp Cys Leu Ala Arg Trp Pro Gly Ser Arg Ala Leu Val Ala Pro
115 120 125
Leu Gly Thr Pro Ala Phe Val Asp Arg Leu Phe Trp Ser Asp Phe Ser
130 135 140
Gly Ser Ile Arg Arg Glu Glu Glu Ala Glu Ala Leu Arg Asp Pro Ile
145 150 155 160
Arg Arg

Claims (18)

1.一种核酸，其在植物中转录后或翻译后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，特征在于所述核酸包含核苷酸序列，其
(i)选自SEQ ID No:9、11、8、10、1、2、3、4、5、6、7、12、13、14、15、42、43、46、47、49、50、52、53、55、56、57、58、59、60、61和/或62，或这些的功能性片段，或
(ii)与来自(i)的序列互补，或
(iii)与来自(i)的序列至少80％相同，或
(iv)编码具有选自SEQ ID No:23、21、22、16、17、18、19、20、24、25、44、45、48、51、54、63、64和/或65的氨基酸序列的蛋白或所述蛋白的功能性部分，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其功能性部分，或
(vi)与来自(ii)的序列在严格条件下杂交。
2.一种核酸，其在植物中转录后或翻译后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，特征在于所述核酸包含核苷酸序列，其
(I)具有选自SEQ ID No:26、27、28、29、30和/或31的序列或其功能性片段，或
(ii)与来自(i)的序列互补，或
(iii)与来自(i)的序列至少80％相同，或
(iv)编码具有选自SEQ ID No:32、33和/或34的氨基酸序列的蛋白或所述蛋白的功能性部分，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其功能性部分，或
(vi)与来自(ii)的序列在严格条件下杂交。
3.一种核酸，其在植物中转录后或表达后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，特征在于所述核酸编码具有双链部分的RNA，其中所述双链部分的至少一条链具有这样的核苷酸序列，其具有编码序列的至少14个连续核苷酸，与以下核酸同源
(i)具有有义或反义方向的选自SEQ ID No:26、27、28、29、30和/或31的序列或其功能性片段的核酸，或
(ii)与来自(i)的序列互补的核酸，或
(iii)与来自(i)的序列至少80％相同的核酸，或
(iv)编码具有选自SEQ ID No:32、33和/或34的氨基酸序列的蛋白或所述蛋白的功能性部分的核酸，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其部分的核酸，或
(vi)与来自(ii)的序列在严格条件下杂交的核酸。
4.一种核酸，其在植物中转录后或表达后，适于介导单倍体诱导系的特性或适于增加单倍体诱导系的诱导能力，特征在于所述核酸编码具有双链部分的RNA，其中所述双链部分的至少一条链具有这样的核苷酸序列，其具有内含子序列的至少14个连续核苷酸，与以下核酸同源
(i)具有有义或反义方向的选自SEQ ID No:9、8、1、6、12、13、26、30、42、43、46、55、58和/或60的序列或其功能性片段的核酸，或
(ii)与来自(i)的序列互补的核酸，或
(iii)与来自(i)的序列至少80％相同的核酸，或
(iv)编码具有选自SEQ ID No:23、21、22、16、17、18、19、20、24、25、44、45、48、63、64和/或65，或选自SEQ ID No:32、33和/或34-35的氨基酸序列的蛋白或所述蛋白的部分的核酸，或
(v)编码根据(iv)的蛋白的同源物、类似物或直系同源物或其部分的核酸，或
(vi)与来自(ii)的序列在严格条件下杂交的核酸。
5.一种载体，其包含权利要求1-4之一的核酸。
6.一种宿主细胞，其包含权利要求1-4之一的核酸或权利要求5的载体。
7.一种转基因植物细胞，其包含权利要求1-4之一的核酸或权利要求5的载体。
8.转基因植物或其部分，其包含权利要求7的细胞。
9.一种用于产生适于用作单倍体诱导系的植物的方法，包括以下步骤：
A)诱变植物细胞且随后从所诱变的植物细胞再生植物或诱变植物，和
B)鉴定植物A)，其在与权利要求1-5之一的核酸相同的内源DNA序列中具有至少一个突变，或在所述内源DNA序列的调控序列中具有至少一个突变，所述突变导致与未诱变野生型植物相比所述内源DNA序列在所鉴定的植物中转录或表达率的变化，或者与未诱变野生型植物相比所述内源DNA序列编码的蛋白或多肽在所鉴定的植物中活性或稳定性的变化，
其中所述至少一个突变导致所鉴定的植物中单倍体诱导系特性被介导或单倍体诱导系的诱导能力被增加。
10.通过权利要求9方法产生的或能够通过权利要求9的方法产生的植物或所述植物的部分。
11.一种分离在植物中介导单倍体诱导系特性或增加单倍体诱导系的诱导能力的核酸的方法，包括以下步骤：
A)根据权利要求9的方法产生植物，或提供权利要求10的植物，
B)从来自A)的植物的基因组分离核酸，所述核酸包含具有所述至少一个突变的内源DNA序列。
12.通过权利要求11的方法获得的或能够通过权利要求11的方法获得的核酸。
13.一种用于产生适于用作单倍体诱导系的转基因植物的方法，包括以下步骤：
A)提供包含权利要求1-4或12之一的核酸，
B)通过导入来自A)的核酸转化植物细胞，
C)从来自B)的经转化的植物细胞再生转基因植物，和
D)通过改变的表达模式从C)鉴定转基因植物，其中单倍体诱导系的特性被介导或单倍体诱导系的诱导能力被增加。
14.通过权利要求13的方法产生的或能够通过权利要求13的方法产生的转基因植物或所述转基因植物的部分。
15.一种用于产生单倍体植物的方法，包括以下步骤：
A)使权利要求8、10或14之一的植物与相同属的植物杂交，
B)选择受精的单倍体种子或胚，和
C)从来自B)的种子或胚再生单倍体植物。
16.通过权利要求15的方法获得的或能够通过权利要求15的方法获得的单倍体植物。
17.权利要求1-4或12之一的核酸或权利要求15的载体在植物中的用途，其用于介导单倍体诱导系的特性，或用于增加单倍体诱导系的诱导能力，或用于产生适于用作单倍体诱导系的植物或转基因植物。
18.权利要求8、10或14之一的植物在产生单倍体受精的种子或胚或产生单倍体植物中的用途。
CN201580061712.7A 2014-11-12 2015-11-12 单倍体诱导系 Pending CN107223155A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102014016667.8A DE102014016667B4 (de) 2014-11-12 2014-11-12 Haploideninduktoren
DE102014016667.8 2014-11-12
PCT/EP2015/076469 WO2016075255A1 (de) 2014-11-12 2015-11-12 Haploideninduktoren

Publications (1)

Publication Number Publication Date
CN107223155A true CN107223155A (zh) 2017-09-29

Family

ID=54557390

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580061712.7A Pending CN107223155A (zh) 2014-11-12 2015-11-12 单倍体诱导系

Country Status (10)

Country Link
US (2) US10631482B2 (zh)
EP (1) EP3218494A1 (zh)
CN (1) CN107223155A (zh)
AR (1) AR102608A1 (zh)
BR (1) BR112017008518B1 (zh)
CA (1) CA2967127C (zh)
CL (1) CL2017001176A1 (zh)
DE (1) DE102014016667B4 (zh)
PE (1) PE20170781A1 (zh)
WO (1) WO2016075255A1 (zh)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109554387A (zh) * 2018-01-16 2019-04-02 中国农业大学 一种诱导产生水稻母本单倍体的方法
CN109705199A (zh) * 2019-02-01 2019-05-03 山东农业大学 Fan蛋白在调控植物根长中的应用
CN109837295A (zh) * 2018-07-06 2019-06-04 先正达参股股份有限公司 一种用基因编辑创制的水稻单倍体诱导系及其创制方法和应用
WO2022117024A1 (zh) * 2020-12-03 2022-06-09 中国农业大学 孤雌生殖单倍体诱导基因及其应用
CN115843674A (zh) * 2021-08-04 2023-03-28 北大荒垦丰种业股份有限公司 玉米单倍体诱导系的选育方法及其应用
CN116034870A (zh) * 2023-01-10 2023-05-02 中国农业科学院作物科学研究所 一种通过含有可视化标记的小麦基因编辑突变体诱导的单倍体植株的筛选方法
CN116267598A (zh) * 2023-04-07 2023-06-23 云南省农业科学院粮食作物研究所 一种提高小麦单倍体得胚率的激素处理方法

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10448588B2 (en) 2013-03-15 2019-10-22 Syngenta Participations Ag Haploid induction compositions and methods for use therefor
EP3091076A1 (en) 2015-05-07 2016-11-09 Limagrain Europe Polynucleotide responsible of haploid induction in maize plants and related processes
CN107920486B (zh) 2015-06-30 2023-06-20 明尼苏达大学董事会 用于加速基因组编辑的单倍体诱导系
RU2771141C2 (ru) * 2015-11-18 2022-04-27 Зингента Партисипейшнс Аг Композиции для индукции гаплоидии и способы их применения
US10519456B2 (en) 2016-12-02 2019-12-31 Syngenta Participations Ag Simultaneous gene editing and haploid induction
AU2017366760B2 (en) 2016-12-02 2024-03-21 Syngenta Participations Ag Simultaneous gene editing and haploid induction
CN106701803B (zh) * 2017-01-13 2019-03-08 中国农业大学 玉米母本单倍体主效诱导基因及应用
CN108192912B (zh) * 2017-01-13 2019-05-07 中国农业大学 一种诱导产生玉米母本单倍体的方法
EP3366778A1 (de) 2017-02-28 2018-08-29 Kws Saat Se Haploidisierung in sorghum
WO2019234129A1 (en) 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
AR118995A1 (es) 2019-05-25 2021-11-17 Kws Saat Se & Co Kgaa Mejorador de la inducción de haploides
CN116574164A (zh) * 2022-03-04 2023-08-11 华中农业大学 单倍体诱导基因的应用
CN116515829A (zh) * 2023-04-06 2023-08-01 吉林农业大学 lncRNA43234基因序列及其克隆方法和在调控植株脂肪酸合成代谢中的应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
US20120017292A1 (en) * 2009-01-16 2012-01-19 Kovalic David K Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgene plants with enhanced agronomic traits
ES2774722T3 (es) * 2009-10-06 2020-07-22 Univ California Generación de plantas haploides y fitomejoramiento mejorado
AU2010327998B2 (en) 2009-12-10 2015-11-12 Iowa State University Research Foundation, Inc. TAL effector-mediated DNA modification
WO2012030893A1 (en) 2010-08-31 2012-03-08 Monsanto Technology Llc Molecular markers associated with haploid induction in zea mays
AU2012338455A1 (en) 2011-11-14 2014-05-22 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and a method for making the same
WO2014110274A2 (en) * 2013-01-09 2014-07-17 Regents Of The University Of California A California Corporation Generation of haploid plants
US9677082B2 (en) * 2013-03-15 2017-06-13 Syngenta Participations Ag Haploid induction compositions and methods for use therefor
US20160017349A1 (en) * 2013-03-15 2016-01-21 Pioneer Hi-Bred International, Inc. Maize microrna sequences and targets thereof for agronomic traits
EP3091076A1 (en) 2015-05-07 2016-11-09 Limagrain Europe Polynucleotide responsible of haploid induction in maize plants and related processes
EP3366778A1 (de) * 2017-02-28 2018-08-29 Kws Saat Se Haploidisierung in sorghum

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109554387A (zh) * 2018-01-16 2019-04-02 中国农业大学 一种诱导产生水稻母本单倍体的方法
CN109837295A (zh) * 2018-07-06 2019-06-04 先正达参股股份有限公司 一种用基因编辑创制的水稻单倍体诱导系及其创制方法和应用
CN109705199A (zh) * 2019-02-01 2019-05-03 山东农业大学 Fan蛋白在调控植物根长中的应用
CN109705199B (zh) * 2019-02-01 2021-10-08 山东农业大学 Fan蛋白在调控植物根长中的应用
WO2022117024A1 (zh) * 2020-12-03 2022-06-09 中国农业大学 孤雌生殖单倍体诱导基因及其应用
CN114656546A (zh) * 2020-12-03 2022-06-24 中国农业大学 孤雌生殖单倍体诱导基因及其应用
CN114656546B (zh) * 2020-12-03 2023-09-19 中国农业大学 孤雌生殖单倍体诱导基因及其应用
CN115843674A (zh) * 2021-08-04 2023-03-28 北大荒垦丰种业股份有限公司 玉米单倍体诱导系的选育方法及其应用
CN115843674B (zh) * 2021-08-04 2024-07-26 北大荒垦丰种业股份有限公司 玉米单倍体诱导系的选育方法及其应用
CN116034870A (zh) * 2023-01-10 2023-05-02 中国农业科学院作物科学研究所 一种通过含有可视化标记的小麦基因编辑突变体诱导的单倍体植株的筛选方法
CN116267598A (zh) * 2023-04-07 2023-06-23 云南省农业科学院粮食作物研究所 一种提高小麦单倍体得胚率的激素处理方法
CN116267598B (zh) * 2023-04-07 2024-02-09 云南省农业科学院粮食作物研究所 一种提高小麦单倍体得胚率的激素处理方法

Also Published As

Publication number Publication date
US20170327832A1 (en) 2017-11-16
US20200229367A1 (en) 2020-07-23
CA2967127A1 (en) 2016-05-19
DE102014016667B4 (de) 2024-03-07
CL2017001176A1 (es) 2017-12-01
PE20170781A1 (es) 2017-07-04
WO2016075255A1 (de) 2016-05-19
EP3218494A1 (de) 2017-09-20
BR112017008518A2 (pt) 2018-01-09
US10631482B2 (en) 2020-04-28
CA2967127C (en) 2024-01-09
BR112017008518B1 (pt) 2023-11-21
DE102014016667A1 (de) 2016-05-12
AR102608A1 (es) 2017-03-15

Similar Documents

Publication Publication Date Title
CN107223155A (zh) 单倍体诱导系
AU2020223680B2 (en) Plant regulatory elements and uses thereof
KR102607893B1 (ko) 지베렐린 대사의 조작을 통해 저신장 식물의 수확량을 증가시키기 위한 방법 및 조성물
CN106998665B (zh) 单倍体植物的产生
AU2013312198B2 (en) Fluorescence activated cell sorting (FACS) enrichment to generate plants
RU2735254C2 (ru) Снижение табак-специфичных нитрозаминов в растениях
CN101365786B (zh) 具有改良的生长特征的植物及其生产方法
AU2019246847B2 (en) Qtls associated with and methods for identifying whole plant field resistance to sclerotinia
KR102156719B1 (ko) 식물 조절 요소 및 그의 용도
CN108882689A (zh) 烟草植物体及其制备方法
KR20170020416A (ko) 식물 내의 질산염 함량 조절
CN115175556A (zh) 与大豆中锈病抗性相关联的新颖的遗传基因座
CN110546266A (zh) 高粱的单倍体化
CN111542610A (zh) 精确基因组编辑的新策略
CN111295447A (zh) 玉米优良事件mzir098
CA2492136A1 (en) Nuclear fertility restorer genes and methods of use in plants
CN106471008B (zh) 棕榈Mantle表型检测
CN114514321A (zh) 调节植物中的糖和氨基酸含量（sultr3）
KR20220165764A (ko) 니코티아나 타바쿰에서 니코틴 수준을 조절하는 방법
CN115867565A (zh) 用于改善对大豆锈病的抗性的方法
AU2008200749A1 (en) Promoters for regulation of plant gene expression
RU2820183C2 (ru) Устойчивость к растрескиванию стручков у растений рода brassica
CN115135142A (zh) 控制籽粒大小和粒重的方法
CA2503837A1 (fr) Sequences peptidiques ppr capables de restaurer la fertilite male de plantes porteuses d&#39;un cytoplasme inducteur de sterilite male
RU2796556C2 (ru) Регуляторные элементы растений и их применение

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination