CN108866026A - High conversion selenium-rich peanuts bud Nattokinase production method - Google Patents
High conversion selenium-rich peanuts bud Nattokinase production method Download PDFInfo
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Abstract
The invention discloses high conversion selenium-rich peanuts bud Nattokinase production methods,Advantage is,The effect of its anticancer of the selenium-rich peanuts bud of high conversion and resisting cardiovascular disease is significantly better than common selenium peanut sprout,The content of resveratrol is higher by tens times than peanut or grape,It accesses natto strain step by step on selenium peanut sprout and has significantly improved effect again,The conversion ratio of organic selenium is improved simultaneously,The specifically soybean of substep addition 15%-50% in selenium peanut sprout,Improve the reproduction speed of Bacillus natto,Solve the problems, such as that unconverted sodium selenite has inhibition to breed Bacillus natto,Selenium peanut sprout itself has excellent anti-aging effects,Inorganic selenium is further absorbed by Bacillus natto and organic transformation,So that the content of inorganic selenium reduces about 5 times,Safety and comprehensive health-care effect also significantly improve,Animal wastes are replaced to solve the problems, such as that peanut sprout is smelly with the wheat bran of 920 combining with fermentation of auximone,It is to prevent and treat angiocarpy,It is antitumor,The immune excellent food of enhancing.
Description
Technical field
The present invention relates to healthy food applied technical fields more particularly to high conversion selenium-rich peanuts bud Nattokinase to produce
Method.
Technical background
With the acceleration of people's life rhythm with the deterioration of living environment, the disease incidence of various diseases greatly improved, especially
It is people's health to be endangered increasingly with cardiovascular and cerebrovascular disease caused by thrombus and cancer, and have the tendency that becoming younger.For
This it has been developed that a variety of anticancers and resisting cardiovascular disease drug and food, for example, selenium element being capable of toxin expelling and tool
There are enhancing immunity of organisms and anti-cancer function；But human body is merely able to absorb organic selenium, and inorganic selenium is difficult to be absorbed by the body,
But toxicity is more much greater than organic selenium；Even organic selenium is not all to be absorbed by the body yet, because absorption of human body is most
Be plant selenoprotein, followed by absorb microorganism conversion selenium.Plant selenoprotein selenium crop bud (selenium malt, selenium bean sprouts and
Selenium peanut sprout) in content it is most.In fact the conversion ratio that current selenium product is converted into organic selenium be not it is very high, made with selenium
For object bud, the conversion ratio of selenium only has 50% or so in selenium malt, and the conversion ratio of selenium bean sprouts and selenium peanut sprout is also in 50%-
Between 60%, this is because only having plumule and young shoot to be converted to for organic selenium in selenium crop bud, other parts are only formed sediment
Powder has suctioned selenate, due to remaining the inorganic selenium of 40%-50%, so toxicity is still big, doctor and family numbers of patients
All the safety problem caused by toxicity is had a lingering fear；Although microorganism can reach 80% to the conversion ratio of selenium, therein
Selenium in Plants protein content is relatively low so assimilation effect is not equally also high.Present American improves the organic selenium purity of selenium yeast,
Price has but gone up tens times, and the product price of 100g is unexpectedly up to 2000 yuan or more, and the effect is unsatisfactory.Therefore, it develops
High conversion rate, be easy to absorb, low cost organic selenium product it is most important.
It is found that peanut is after germination, Resveratrol content therein is than former peanut and than the white black false hellebore in grape wine
Alcohol will be higher by tens times, and resveratrol has the function of reducing blood lipid and prevention and treatment cardiovascular and cerebrovascular disease.If taken with selenium peanut sprout
The peanut sprout of generation routine, then health-care effect effect for the people in selenium deficiency area can enhance many times.Therefore number of patent application
201310239557.6 all disclosing the breeding method of selenium-rich peanuts bud with number of patent application 201610947904.4；On but
The organic transformation rate for stating 2 kinds of selenium peanut sprouts is not high, generally between 50%-60%, this is because the plumule of only peanut and children
Bud is converted to for organic selenium, and other parts are that seed has been suctioned selenate, remains the inorganic of 40%-50%
Selenium is so that toxicity is still bigger than normal；The organic selenium for safety even only improve 10% be also people thirst for.And
2 application documents all add animal wastes, harmful bacteria can be bred under the germination environment, and peanut sprout can absorb animal excreta
Just make peanut sprout smelly；It is exactly carcinogenic aflatoxins easy to breed there are one harmful factor, this is because peanut is sent out
The environment of bud and the breeding environment of aflatoxins are close, and the animal wastes of addition also have the factor for accelerating aflatoxins breeding, yellow
The toxin that aspergillin generates is very strong carcinogen, and even high-temperature sterilization is also difficult to decompose this toxin；The patent application
The measure that file does not all prevent aflatoxins from breeding.And make people intractable, sodium selenite can inhibit peanut to germinate, especially
It is to there is a kind of thermophilic selenium bacterium to breed under selenium environment, which can destroy the structure of young shoot, affect flower again
The growth sprouted.
In addition, the fermented soya bean that there is history of more than one thousand years in China have thrombus well, certain Japanese is in fermented soya bean
On the basis of have developed Nattokinase, thrombolytic effect is stronger, but Japanese Natto has bitter stink, has seriously affected mouthfeel, thus
People have to add seasoning to improve the mouthfeel of natto, such as add the inulin etc. of pungent zanthoxylum powder and fragrance weight,
But improving taste, the effect is unsatisfactory, and seasoning but reduces the activity and concentration of Nattokinase, so that effect subtracts greatly.Cause
This, patent " preparation method (application number 201410102876.7) of peanut meal natto sauce " carries out peanut meal using Bacillus natto
Short time continuously ferments, and can be effectively improved the mouthfeel and taste of existing natto.But peanut meal during storage easily by
The pollution of mould, is especially polluted by aflatoxins, and problem is that contaminated peanut meal is difficult in early metaphase by it has been found that i.e.
It is that high-temperature sterilization can not destroy its toxicity.
For this purpose, people thirst for needing it is a kind of have can thrombus, and have concurrently cancer-resisting, in good taste, effect is good,
Organic selenium high conversion rate, toxicity are low, are not easy the product polluted by mould especially aflatoxins.
Summary of the invention
The purpose of the present invention has thrombus in a kind of completely new kind, while having cancer-resisting, in good taste, effect concurrently
Fruit is good, organic selenium high conversion rate, toxicity is low, the characteristics of being not easy by mould contamination, that is, high conversion and has thrombolysis and anti-
The selenium peanut sprout Nattokinase product of cancer effect, product of the invention and production method are summarised as：First step configuration and immersion, the
Selenium peanut sprout is made in two steps, and third step is selenium peanut sprout to be cleaned sterilizing cooling, and the 4th step is to access 1.5% in selenium peanut sprout
Natto strain, the level-one selenium peanut bud kinase with thrombolytic effect is made, the 5th step is formed into the second level with thrombolytic effect
Selenium peanut bud kinase, while low temperature drying and crushing packing.The bitter stink of Nattokinase is not present in the product, but has peanut sprout
Faint scent taste, this is very difficult to user.Again since Bacillus natto has very strong turn to the inorganic selenium in selenium peanut sprout
Change effect, largely can be converted to remaining sodium selenite for organic selenium, so reducing toxicity at double, significantly mention
High Product Safety solves the problems, such as that people make great efforts always but organic selenium conversion ratio is low in the selenium peanut sprout that does not solve.
However to obtain it is this there is thrombolysis and antitumaous effect selenium peanut bud kinase not a duck soup, need to solve in production
Certainly many technical problems mainly include：Peanut carcinogenic aflatoxins problem easy to breed in germination process；Add animal excreta
The problem that harmful bacteria will be bred while making peanut sprout smelly, it is insufficient that there is fertility again if not adding animal wastes
Problem, the thermophilic selenium bacterium that pink is bred under selenium environment hinder the growth question of peanut sprout, and selenium peanut sprout organic selenium conversion ratio is not
The problem of high problem and sodium selenite can inhibit Bacillus natto to breed.
Solve the problems, such as that selenium peanut sprout organic selenium conversion ratio is not high, method is the access natto strain in selenium peanut sprout, no
Selenium peanut sprout is only enabled to be provided with very strong thrombolytic effect, and Bacillus natto can transform into residual sodium selenite
Machine selenium is more than or equal to 80% according to related record conversion ratio, and the inorganic selenium not being converted in other words is only left 20%, and toxicity reduces
About 5 times.
Solve the problems, such as that peanut carcinogenic aflatoxins easy to breed, method that the present invention takes in germination process are in flower
Raw to be passed through ozone in solution that sodium selenite impregnates, ozone can effectively kill the Aspergillus flavus inside and outside peanut, also include
Saccharomycete and other miscellaneous bacterias are killed, and ozone does not pollute, because ozone is decomposed into oxygen quickly；Test proves to be passed through smelly
Oxygen can make peanut sprout grow sturdy, and it is primary to be specifically passed through ozone every 40-60 minutes, be passed through the time every time 30 seconds, and by
It is short in the time being passed through, so not will cause ozone excess.Simultaneously because auximone 920 is inherently able to suppress aspergillus flavus
The breeding of element, and auximone 920 replaces the measure of animal wastes to accelerate the speed that peanut germinates, then from the time
Also limit the breeding of aflatoxins.
Solve the problems, such as that the animal wastes of addition can breed harmful bacteria under the germination environment and peanut sprout is smelly asks
Topic, method are, with wheat bran and soya-bean cake and water with 100: 60: 50 ratio, saccharomycetes to make fermentation to be added, and then dry and sterilizing is matched
Set nutrients of the concentration 1% as peanut sprout, in conjunction with auximone 920 can rapidly vernalization, coordinate plant growth element 920
Concentration be 200PPM；Facts have proved that the fertility effect of animal wastes is slow so that germination process is elongated, the process of germination more it is long then
More it is subject to fungal contamination.Certainly the present invention does not use any chemical fertilizer, since 920 be auximone, the side effect to people
Very low, well below chemical fertilizer, while dosage is few, so the side effect to people can be ignored.
Solve the problems, such as that unconverted sodium selenite has inhibition to breed Bacillus natto, method is that substep adds in selenium peanut sprout
Enter the soybean of 15%-50%, the reproduction speed of Bacillus natto can be increased substantially, test proves, when the soybean amount of addition is lower than
Effect is undesirable when 10%, and when soybean amount is added and is higher than 50%, mouthfeel is obviously deteriorated, and soybean is added using point two steps in the present invention
Measure can significantly improve the reproduction speed of Bacillus natto, the first step is that the soybean after impregnating is added in selenium peanut sprout, is pressed
It is admixed according to peanut sprout than soybean 100: 50, natto strain is then added.Second step is big after immersion is added in selenium peanut sprout
Beans are admixed according to selenium peanut sprout and soybean ratio 100: 20, and the natto mycelia obtained from the first step is then added, and observation discovery is received
The growth of beans bacterium is vigorous, in good taste.
The effect (1.2% natto strain is added) after soybean is added in table 1 in selenium peanut sprout
For this purpose, being summarized as production high conversion and steps are as follows with the selenium peanut bud kinase of thrombolytic effect：
(1) seed selection and immersion select to be immersed in selenium concentration without damaged and healthy and strong peanut seed without mildew as 10mg/L's
It is impregnated 20-24 hours in selenium solution, temperature is controlled at 22-26 DEG C, while interval is passed through ozone, is passed through every 40-60 minutes smelly
Oxygen is primary, is passed through time 30-40 second every time；
(2) soaked seed is placed in peanut Germinator and germinates by germination process, and temperature is controlled at 25 DEG C -28
DEG C, every selenium solution 30 seconds that 30 minutes circulated sprinkling selenium concentrations are 10mg/L, with wheat bran and soya-bean cake and water with 100: 60: 50
Saccharomycetes to make fermentation is added in ratio, then dry and sterilizing, nutrients of the configuration concentration 1% as peanut sprout, in conjunction with plant growth
Element 920 can rapidly vernalization, the concentration of coordinate plant growth element 920 is 200PPM；Peanut fast germination at this time, until young shoot is long
Peanut sprout is taken out when spending 1-2cm；Peanut Germinator is restructured with bean sprout growing machine, and restructuring process is replacement temperature controller, control temperature
25 DEG C -30 DEG C of degree, it is elongated that temperature drift will lead to young shoot, and Aspergillus flavus is easy to cause to breed, and the relatively low then germination of temperature is slow
Slowly, and the control temperature of bean sprout growing machine is constant at 31 DEG C；
(3) level-one selenium peanut sprout matrix is prepared, selenium peanut and young shoot are cleaned to the mucus of secretion, soak with ozone solution with clear water
It kills the mould bred in pregermination procedure within 10 minutes, pulls out and drain away the water；Simultaneously take soybean soaking 20 hours, by selenium peanut sprout with
For soybean after immersion according to 100: 60 blendings, tinning conventional sterilant is cooled to 40 DEG C or less；
(4) natto strain is accessed on a grade matrix and is bred, inoculum concentration 1.5%, 30 DEG C -38 DEG C of inoculation temperature,
Breeding 24-36h is protected from light under 36-39 DEG C of environment, it is whole for breeding to cover with white hypha, obtain level-one substrate mycelium.
(5) mucus that peanut and young shoot are cleaned to secretion with clear water, uses soak with ozone solution 10 minutes immediately, kills vernalization
The mould bred in journey, pulls out and drains away the water；Simultaneously soybean soaking 20 hours, by selenium peanut sprout and impregnate after soybean according to
100: 20 blendings, tinning conventional sterilant are cooled to 40 DEG C or less；
(6) secondary matrix is inoculated into using level-one substrate mycelium as strain in secondary matrix access level-one natto mycelia
On, inoculum concentration is the 25% of secondary matrix, while breeding -36h for 24 hours is protected from light under 36 DEG C of -39 DEG C of environment, to cover with white hypha
Eventually for breeding, the secondary matrix mycelium of organic selenium high conversion is obtained, Bacillus natto can generate a large amount of natto while breeding
Kinases, it is directly edible as non-staple foodstuff, it can also be to pack to obtain powdery pouch finished product, warm water punching food under low temperature drying gnotobasis.
Beneficial effect：It is an advantage of the current invention that its anticancer of the selenium-rich peanuts bud of high conversion and resisting cardiovascular disease
Effect be significantly better than common selenium peanut sprout, the content of resveratrol is higher by tens times than peanut or grape, in selenium peanut
Natto strain is accessed above bud step by step and has significantly improved effect again, while improving the conversion ratio of organic selenium, specifically substep exists
The soybean of 15%-50% is added in selenium peanut sprout, improves the reproduction speed of Bacillus natto, solves unconverted sodium selenite pair
Bacillus natto have inhibition breed the problem of, selenium peanut sprout itself has excellent anti-aging effects, by Bacillus natto to inorganic selenium into
The absorption and organic transformation of one step, so that the content of inorganic selenium reduces about 5 times, safety and comprehensive health-care effect are also at double
Improve, replace animal wastes to solve the problems, such as that peanut sprout is smelly with the wheat bran of 920 combining with fermentation of auximone, be prevention and
Treat excellent food cardiovascular, antitumor, that enhancing is immune.
Specific embodiment
Method of the invention is further illustrated below with reference to embodiment.
High conversion selenium-rich peanuts bud Nattokinase production method includes in technical solution：Seed selection with impregnate, germinate, match
Level-one selenium peanut sprout matrix processed and access natto strain, secondary matrix blending soybean, second level selenium peanut sprout matrix access level-one selenium
Peanut sprout concentration mycelia.
Specific embodiment is as follows：
(1) seed selection and immersion select to be immersed in selenium concentration without damaged and healthy and strong peanut seed without mildew as 10mg/L's
It is impregnated 20-24 hours in selenium solution, temperature is controlled at 22-26 DEG C, while interval is passed through ozone, is passed through every 40-60 minutes smelly
Oxygen is primary, is passed through time 30-35 second every time；
(2) it germinates, soaked seed is placed in peanut Germinator and is germinateed, temperature is controlled at 25 DEG C -28 DEG C, often
Every selenium solution 30 seconds that 30 minutes circulated sprinkling selenium concentrations are 10mg/L, with wheat bran and soya-bean cake and water with 100: 60: 50 ratio,
Saccharomycetes to make fermentation is added, then dry and sterilizing, nutrients of the configuration concentration 1% as peanut sprout, in conjunction with auximone 920
Can rapidly vernalization, the concentration of coordinate plant growth element 920 is 200PPM；Peanut fast germination at this time, until young shoot length 1-
When 2cm eventually for germination；
(3) level-one selenium peanut sprout matrix is prepared, selenium peanut and young shoot are cleaned to the mucus of secretion, soak with ozone solution with clear water
It kills the mould bred in pregermination procedure within 10 minutes, pulls out and drain away the water；Soybean soaking 20 hours simultaneously, by selenium peanut sprout and leaching
For soybean after bubble according to 100: 60 blendings, tinning conventional sterilant is cooled to 40 DEG C or less；
(4) the access natto strain on a grade matrix, inoculum concentration 1.5%, 30 DEG C -38 DEG C of inoculation temperature, at 36-39 DEG C
Breeding 24-36h is protected from light under environment, it is whole for breeding to cover with white hypha, obtain level-one substrate mycelium.
(5) secondary matrix admixes soybean, and peanut and young shoot are cleaned to the mucus of secretion with clear water, use soak with ozone solution immediately
It 10 minutes, pulls out and drains away the water；Soybean soaking 20 hours simultaneously, the soybean by selenium peanut sprout and after impregnating was mixed according to 100: 20
With tinning conventional sterilant is cooled to 40 DEG C or less；
(6) secondary matrix is inoculated into using level-one substrate mycelium as strain in secondary matrix access level-one natto mycelia
On, inoculum concentration is the 25% of secondary matrix, while breeding -36h for 24 hours is protected from light under 36-39 DEG C of environment, is to cover with white hypha
Breeding eventually, obtains the secondary matrix mycelium of organic selenium high conversion, and Bacillus natto can generate a large amount of natto while breeding and swash
Enzyme, it is directly edible as non-staple foodstuff, it can also be to pack to obtain powdery pouch finished product under low temperature drying gnotobasis.
Appearance test, appearance are creamy white, and have the fragrance of peanut, mouthfeel can, without uncomfortable smell.
Peanut Germinator is characterized in being restructured with bean sprout growing machine, and restructuring process is replacement temperature controller, the control of bean sprout growing machine
Temperature processed is changed to 25 DEG C -30 DEG C by 31 DEG C, and it is elongated that temperature drift will lead to young shoot, and Aspergillus flavus is easy to cause to grow
Raw, the relatively low then germination of temperature is slow.
Claims (2)
1. high conversion selenium-rich peanuts bud Nattokinase production method of the present invention, feature include the following steps：
(1) seed selection and immersion select that it is molten for the selenium of 10mg/L to be immersed in selenium concentration without damaged and healthy and strong peanut seed without mildew
It is impregnated 20-24 hours in liquid, temperature is controlled at 22-26 DEG C, while interval is passed through ozone, is passed through ozone one every 40-60 minutes
It is secondary, it is passed through time 30-35 second every time；
(2) soaked seed is placed in peanut Germinator and germinates by germination process, and temperature is controlled at 25 DEG C -28 DEG C, often
It is selenium solution 30 seconds of 10mg/L every 30 minutes circulated sprinkling selenium concentrations, auximone 920 is added in the solution, adjusts concentration
For 0.2g/L, until taking out peanut sprout when young shoot length 1-2cm；Peanut Germinator is restructured with bean sprout growing machine, and restructuring process is
Temperature controller is replaced, controls 25 DEG C -30 DEG C of temperature；
(3) level-one selenium peanut sprout matrix is prepared, selenium peanut and young shoot are cleaned to the mucus of secretion with clear water, soak with ozone solution 10 divides
Clock kills the mould bred in pregermination procedure, pulls out and drains away the water；Soybean soaking 20 hours simultaneously, by selenium peanut sprout and after impregnating
Soybean according to 100: 60 blendings, tinning conventional sterilant is cooled to 40 DEG C or less；
(4) natto strain is accessed on a grade matrix and is bred, inoculum concentration 1.5%, 30 DEG C -38 DEG C of inoculation temperature, in 36-39
It is protected from light breeding 24-36h under DEG C environment, to cover with white hypha as breeding eventually, obtains level-one substrate mycelium.
(5) mucus that peanut and young shoot are cleaned to secretion with clear water, uses soak with ozone solution 10 minutes immediately, pulls out and drain away the water；
Soybean soaking 20 hours simultaneously, according to 100: 20 blendings, tinning conventional sterilant was cooled to the soybean by selenium peanut sprout and after impregnating
40 DEG C or less；
(6) it is inoculated on secondary matrix in secondary matrix access level-one natto mycelia using level-one substrate mycelium as strain,
Inoculum concentration is the 25% of secondary matrix, while breeding -36h for 24 hours is protected from light under 36-39 DEG C of environment, is numerous to cover with white hypha
End is grown, the secondary matrix mycelium of organic selenium high conversion is obtained, Bacillus natto can generate a large amount of Nattokinase while breeding,
It is directly edible as non-staple foodstuff, it can also be to pack to obtain powdery pouch finished product under low temperature drying gnotobasis.
2. peanut Germinator according to claim 1, it is characterized in that being restructured with bean sprout growing machine, restructuring process is replacement temperature
Device is controlled, the control temperature of bean sprout growing machine, which is changed to 25 DEG C -30 DEG C by 31 DEG C, can become later age Germinator.
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