JP2000224918A - Unsterilized culture solution composition and unsterilized cultivation of mushroom using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an unsterilized culture solution composition and to provide a method for cultivating mushrooms by using the composition. SOLUTION: This composition contains 56.4-62.4 wt.% of granite porphyry, 17.1-18.9 wt.% of calcium, 8.7-9.6 wt.% of a burnt ash of newsprint paper, 4.4-4.8 wt.% of a burnt ash of chaff, 2.2-2.4 wt.% of sugar, 1.3-1.5 wt.% of slaked lime, 1.71-1.89 wt.% of burnt pine needles, 1.3-1.5 wt.% of a charcoal of Quercus acutissima, 0.58-0.67 wt.% of a salt, 0.48-0.53 wt.% of burnt rice straws, 0.26-0.28 wt.% of burnt tobacco leaves, 0.26-0.28 wt.% of yellow soil and 0.17-0.19 wt.% of burnt garlics. The unsterilized culture solution composition is diluted to provide an unsterilized culture solution and a culture medium is then dipped in the resultant unsterilized culture solution to absorb the unsterilized culture solution in the culture medium. The culture medium is immediately bedded when the unsterilized culture solution is absorbed in the culture medium and mushroom spawns are inoculated into the culture medium and cultured.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sterile culture solution composition and a method for sterile cultivation of mushrooms using the same. More particularly, a mushroom culture medium is immersed in a sterile culture solution. The present invention relates to a method for sterile cultivation of mushrooms in which a medium is introduced into the medium as soon as moisture is absorbed into the medium, and then the medium is inoculated with mushrooms and cultured.

[0002]

2. Description of the Related Art In general, diseased spores, nematodes, larvae of mushrooms, eggs, and the like are mixed in waste cotton, rice straw, sawdust, and the like used for culture of mushrooms, and these are removed. Therefore, the medium must be sterilized before inoculating the fungi. In addition, sterilization must be performed in order to change the medium components to a state in which the fungi can be easily used and to soften the medium components.

[0003] However, sterilization requires sterilization equipment such as a sterilizer, a boiler, and a cultivation facility that can withstand sterilization pressure. Also,
When spraying chemical insecticides or fungicides,
Safety can be an issue due to residual toxicity.

[0004]

Therefore, the present inventors can achieve the purpose of sterilization without performing sterilization, and have a lower contamination rate of various bacteria than the method of performing sterilization.
In addition, as a result of repeated studies on methods that can increase the yield of mushrooms, a sterilized culture solution containing barley stone as a main component, which is known to have antibacterial and antifungal effects by strong adsorption power and elute minerals After preparing the medium, the medium for mushroom cultivation is immersed in this sterile culture medium, and when the medium absorbs water, the medium is introduced into the medium, and then the medium is inoculated with mushrooms and cultured. It has been found that the contamination rate is lower than that of the method of sterilizing and culturing, and the yield of mushrooms is high, and furthermore, it is possible to save equipment, fuel, time and the like for sterilization, and to complete the present invention. Reached.

[0005] Accordingly, an object of the present invention is to achieve the purpose of sterilization without sterilization when cultivating mushrooms, and furthermore, the contamination rate of various bacteria is lower than in the method of sterilization.
Another object of the present invention is to provide a sterilized culture solution composition that can increase the yield of mushrooms. Another object of the present invention is to
It is an object of the present invention to provide a method for cultivating a mushroom using the above-mentioned sterilized culture solution composition.

[0006]

That is, the present invention provides 56.4 to 62.4% by weight of barley stone, 17.1 to 18.9% by weight of calcium, and burnt newspaper ash, based on the total weight of the composition. 8.7 to 9.6% by weight, rice husk burnt ash 4.4 to 4.8% by weight, sugar 2.2 to 2.4% by weight, slaked lime 1.3 to 1.
5% by weight, Matsuba-yaki 1.71-1.89% by weight, Charcoal charcoal 1.3-1.5% by weight, salt 0.58-0.67% by weight, Rice straw roasting 0.48-0.53 It is a non-sterile culture solution composition containing 0.26 to 0.28% by weight of tobacco leaf, 0.26 to 0.28% by weight of loess, and 0.17 to 0.19% by weight of garlic.

[0007] The present invention also provides a sterile culture solution obtained by diluting the above-mentioned sterile culture solution to a concentration of 300 to 700 times, immersing the medium in the sterile culture solution, and adding water to the medium. Absorbing the sterile culture solution so that the amount is 70 to 75% by weight, injecting the medium into which the sterile culture solution has been absorbed, inoculating the medium with mushrooms, And culturing the medium inoculated with the fungus at 20 to 25 ° C.

Hereinafter, the present invention will be described in more detail. The non-sterile culture solution composition of the present invention has, as a main component, barley stone which is known as a mysterious mineral substance having an antibacterial action and capable of eluting minerals. This barley stone has a slightly higher pH of 8.7 and a CEC (cation exchange capacity).
Is 9.0 me / 100 g, and has a characteristic significantly lower than that of other mineral modifiers such as zeolite and bentonite. Therefore, barley rice stone promotes mycelial growth of mushrooms and inhibits growth of harmful fungi on mushrooms. It has been found to have an effect, and is included as a main component of a sterile culture solution.

[0009] The sterile culture solution composition of the present invention comprises 56.4 to 62.4% by weight of barley stone based on the total weight of the composition.
Contained in the range. In addition, the sterile culture solution composition of the present invention contains calcium, which acts as a coenzyme for mycelial activity with a buffering action of acidity, as an adjuvant for the above-mentioned maltite, from 17.1 to 17.1 relative to the total weight of the composition. It is contained in the range of 18.9% by weight.

Further, the sterilized culture solution composition of the present invention can prevent invasion of pests due to a peculiar smell and can absorb news paper burnt ash.
7 to 9.6% by weight, rice hulls burn ash 4.4 to 4.8% by weight, which can complement each other with newspaper burnt ash and can promote mycelium activity by absorbing harmful gas, carbon to nitrogen ratio 2.2 to 2.4% by weight of sugar for controlling the amount of water, 1.3 to 1.5% by weight of slaked lime having a buffering action on acidity and an inhibitory activity on the growth of pests and mycelia, and a buffering action on temperature changes during mycelial culture 1.71 to 1.89% by weight of pine needles that can prevent re-heating, charcoal of Kunugi 1.3 to 1.1 that can suppress the growth of harmful bacteria and prevent the endothermic effect in the ground. 5% by weight, 0.58 to 0.67% by weight of a salt having an osmotic inhibitory effect on pest mycelium cells, a rice plant capable of preventing harmful gas and reheating and promoting hyphal growth activity Roasted straw 0.48-0.53
0.26 to 0.28% by weight, tobacco leaf baked which can prevent re-heating and suppress the occurrence of pests
0.2% to 0.28% by weight of loess and can be used to activate the mycelium growth, improve the physical properties of the culture medium, and supply trace elements Garlic can be suppressed 0.
It further contains 17-0.19% by weight. In addition, in the said composition, burnt ash and baking mean what completely carbonized each material.

[0011] In the method of the present invention for sterile cultivation of mushrooms,
300-700 times the concentration of the sterile culture solution,
Preferably, a sterile culture solution diluted 500 times is used. There is no significant difference in the growth of the mycelium depending on the concentration of the sterile culture solution, but the higher the concentration, the denser the hyphal density, and the lower the contamination rate, so it is preferable to prepare a sterile culture solution within the above range. .

In order to cultivate mushrooms in the method of the present invention, the medium must be immersed in a sterilized culture medium having the above-mentioned concentration to absorb the sterilized culture medium. The immersion time of the medium does not affect the hyphal growth, the hyphal density, and the contamination rate, and can be appropriately selected by those skilled in the art.

The medium that can be used in the method of the present invention includes:
There is no particular limitation, and for example, a medium used for cultivation of mushrooms, that is, waste cotton, rice straw, sawdust and the like can be used. When waste cotton is used, a medium in which corn bran is added to Nishin bell cotton in an amount of 20 to 30% (V / V), and beet is added to cotton rind in an amount of 20 to 30% (V / V). 8 pieces of broth, broiled cotton, corn bran and beetroot
A medium mixed at a ratio of 0: 15: 5 (V / V), or cotton rind, corn bran, and beet are 70:15 to 20: 1.
It is preferable to use a medium mixed at a ratio of 0 to 15 (V / V).

When the medium is immersed in a sterile culture medium to absorb the sterile culture medium, the water content of the medium can be adjusted to 70 to 75% by weight. It is preferable from the viewpoint of the degree of contamination.

Further, in the method of the present invention, a medium in which the moisture of the above-mentioned non-sterile culture solution has been absorbed is introduced into the bed, and the injected medium is inoculated with a fungus, and the medium is cultured to mushrooms. Cultivate. The culture temperature at that time is preferably 20 to 25 ° C. When the culture temperature is less than 20 ° C, the hyphal growth is slow, and when it exceeds 25 ° C, the hyphal density is low,
There is a problem that it is easily contaminated by various bacteria. The types of mushrooms that can be applied to the non-sterile cultivation method of the present invention are not particularly limited.

[0016]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail based on examples and test examples, taking flat mushrooms, which are most frequently edible mushrooms, as examples. It is not limited to only.

Test Example 1 [Measurement of hyphal growth, hyphal density and germ contamination by mixing ratio of cotton of Nishin bell and additive material] 59.4 mg of barley stone, 18 mg of calcium, 9.16 mg of burnt ash of newspaper, 4 ash of burnt husk 4 .62mg, sugar 2.3mg, slaked lime 1.42m
g, Matsuba-yaki 1.8 mg, Kunugi charcoal 1.42 mg, salt 0.64 mg, rice straw-yaki 0.52 mg, tobacco leaf-yaki 0.27 mg, loess 0.27 mg, and garlic 0.
After mixing 18 mg, this was prepared in a 500-fold solution (hereinafter, referred to as “sterilized culture solution”).

Next, as shown in Table 1, a medium in which corn bran, beet, and rice bran were mixed with cotton of Nishin bell was immersed in the above-mentioned sterilized culture medium, and immediately after the medium absorbed water, the medium was introduced into the bed. . Hiratake fungus (Spring and Autumn No. 2)
Was inoculated and cultured at 25 ° C. for 10 days, and the hyphal growth, hyphal density and bacterial contamination were investigated. Table 1 shows the results. On the other hand, in the control group in Table 1, the cotton of Nishiki bell was fermented at 60 ° C. for 3 hours to enter the bed, and the entered medium was incubated at 60 ° C. for 1 hour.
After sterilization for 2 hours, further fermentation at 50 ° C. for 2 days, and then inoculating Hiratake fungi and culturing.

[0019]

[Table 1]

As is clear from Table 1, when cultivating flat mushrooms using a non-sterilized culture solution, a medium in which corn bran is mixed with cotton of Nishin bell at a ratio of 20 to 30% has a hyphal growth of 7%.
It is the best, about 6 mm / 10 days, and the hypha density is good, and there is no contamination of various bacteria. Therefore, it can be seen that it is effective for the non-sterile cultivation of the flat mushroom using the non-sterile culture solution.

Test Example 2 [Measurement of hyphal growth, hyphal density and degree of contamination of various bacteria by mixing ratio of cotton rind and additive material] The same as in Test Example 1 except that the medium described in Table 2 was used. After cultivating the flat mushroom by the method, the mycelial growth, the mycelial density and the contamination degree of various bacteria are investigated,
The results are shown in Table 2. On the other hand, in the control group in Table 2, after the cotton rind was fermented at 60 ° C. for 3 hours in the field, the bed was sterilized at 60 ° C. for 12 hours.
After the late fermentation at 50 ° C. for about 2 days, flat mushroom fungi were inoculated and cultured.

[0022]

[Table 2]

As is clear from Table 2, when cultivating flat mushrooms using a non-sterile culture solution, beet was added to the cotton bark in 20 to 3 times.
The medium mixed at a volume of 0% has a mycelial growth of 65 mm / 10
It can be seen that the day was the best, the mycelium density was good, and there was no contamination by various bacteria.

Test Example 3 [Measurement of hyphal growth, hyphal density and miscellaneous bacterial contamination by mixing ratio of Nishiki bell cotton, corn bran and beet] The test was conducted in the same manner as in Test example 1 except that the medium described in Table 3 was used. After cultivating flat mushrooms in the same manner, the growth of mycelium, the density of mycelium, and the degree of contamination of various bacteria were investigated. The results are shown in Table 3. On the other hand, in the control plot in Table 3, after the cotton of Nishiki bell was fermented at 60 ° C. for 3 hours to enter the bed, the entered medium was sterilized at 60 ° C. for 12 hours, and the late fermentation at 50 ° C. for about 2 days. And then inoculated with Hiratake fungi and cultured.

[0025]

[Table 3]

As is clear from Table 3, when cultivating flat mushrooms using a non-sterilized culture solution, cotton, corn bran, and beet of Nishin bell were mixed at a ratio of 80: 15: 5 (V / V). It can be seen that the culture medium has the best hyphae growth of 75 mm / 10 days and the low contamination rate.

Test Example 4 [Measurement of hyphal growth, hyphal density and germ contamination by mixing ratio of cotton rind, corn bran, and beet] Same as Test Example 1 except that the medium described in Table 4 was used. After cultivating the flat mushroom by the method described in the above, the mycelial growth, the mycelial density, and the degree of contamination of various bacteria were investigated. The results are shown in Table 4. On the other hand, in the control group in Table 4, after the cotton rind was fermented at 60 ° C. for 3 hours in the field, the bed was sterilized at 60 ° C. for 12 hours, and the late fermentation was performed at 50 ° C. for about 2 days. After inoculation, the culture was inoculated with a flat mushroom fungus and cultured.

[0028]

[Table 4]

As is evident from Table 4, when cultivating flat mushrooms using a non-sterile culture solution, cotton rind, corn bran,
Beat: 70:15:15 (V / V) or 70:20:
A medium mixed at a ratio of 10 (V / V) has a hyphal growth of 5
9 to 60 mm / 10 days is the best, indicating that there is no contamination by various bacteria.

Test Example 5 [Measurement of hyphal growth, hyphal density and microbial contamination based on the concentration of the sterile culture medium]
After cultivating flat mushrooms in the same manner as in Test Example 1 except that the solution was made up to 2,000-fold, the mycelial growth, the mycelial density and the contamination degree of various germs were investigated. The results are shown in Table 5.

[0031]

[Table 5]

As is evident from Table 5, when the concentration of the sterile culture solution is 300- to 700-fold, it is most effective in terms of hyphal growth, hyphal density and bacterial contamination. In particular, the case of a 500-fold solution was the best.

Test Example 6 [Measurement of hyphal growth, hyphal density and bacterial contamination depending on the water content of the culture medium] Cotton rind, corn bran, and beet were 70: 1.
Culture was performed in the same manner as in Test Example 1 except that a medium mixed at a ratio of 5:15 (V / V) was used, and the water content of the medium was adjusted to the content shown in Table 6. After cultivating mushrooms, investigate the mycelial growth, mycelial density and bacterial contamination,
Table 6 shows the results.

[0034]

[Table 6]

As is clear from Table 6, when cultivating flat mushrooms using a non-sterilized culture solution, the water content of the medium was 70 to 7%.
It can be seen that the adjustment to 5% is most preferable in terms of hyphal growth, hyphal density and bacterial contamination.

Test Example 7 [Measurement of hyphal growth, hyphal density and germ contamination by medium immersion time] Cotton rind, corn bran, and beet were 70: 1.
A flat mushroom was cultivated by culturing in the same manner as in Test Example 1 except that a medium mixed at a ratio of 5:15 (V / V) was used and the medium was immersed for the time shown in Table 7. Thereafter, hyphal growth, hyphal density and degree of contamination were examined. The results are shown in Table 7.

[0037]

[Table 7]

As is clear from Table 7, when cultivating flat mushrooms using a non-sterilized culture solution, the time during which the medium is immersed in the culture solution should not affect the hyphal growth, the hyphal density and the contamination degree of various bacteria. I understand. Therefore, as for the immersion time of the medium, the cultivation time can be shortened by immediately injecting the medium and inoculating the medium with the fungi after the medium absorbs the water.

Test Example 8 [Measurement of hyphal growth, hyphal density and bacterial contamination depending on culture temperature] Cotton rind, corn bran and beet were 70: 15: 1.
After cultivating flat mushrooms by culturing in the same manner as in Test Example 1 except that a culture medium mixed at a ratio of 5 (V / V) was used and the culture temperature was adjusted to the temperature shown in Table 8, The hyphal growth, hyphal density and bacterial contamination were investigated and the results are shown in Table 8.

[0040]

[Table 8]

As is evident from Table 8, it is most preferable to adjust the cultivation temperature to 20 to 25 ° C. from the viewpoint of hyphal growth, hyphal density and contamination degree.

Test Example 9 [Effect of Cultivation Method on Fruit Body] As in Test Example 1, using a medium in which cotton rind, corn bran, and beet were mixed at a ratio of 70:15:15 (V / V). After the completion of the culture, measure the individual weight, handle length, fruiting body size (handle length, handle diameter, umbrella diameter) The results are shown in Table 9. On the other hand, as a control, the same medium as described above was sterilized at 65 ° C for 10 hours, fermented at 55 ° C for 2 days, then inoculated with flat mushrooms, and cultured at 25 ° C. The number of days required for the mushrooms was examined, and after completion of the culture, the individual weight, stem length, and fruit body size (stem length, stem diameter, umbrella diameter) were measured. The results are shown in Table 9.

[0043]

[Table 9]

As is clear from Table 9, when cultivating flat mushrooms by a known method, the yield is 48.2 kg / 3.3 m ² , and when cultivating using a non-sterile culture solution, 50.6 kg is obtained.
kg / 3.3 m ² , which indicates that there is an effect of increasing the yield by about 5%. The flat mushroom cultivated by the cultivation method of the present invention has an individual body weight of 17.2 g and is 1% by the conventional method.
It is about 27% heavier than 3.5g.

Test Example 10 [Effects of pests and insects by cultivation method] After cultivating flat mushrooms in the same manner as in Test Example 9, the degree of occurrence of blue mold and bacterial brown spot was investigated. The results are shown in FIG.

[0046]

[Table 10]

As is clear from Table 10, when flat mushrooms were cultivated using a non-sterilized culture solution, blue mold disease occurred at 1 to 5% in fungal beds and box cultivation. Thus, it can be seen that when a non-sterile culture solution is used, it is not necessary to separately use an insecticide.

Example 1 59.4 mg of barley stone, 18 mg of calcium, 9.16 mg of burnt newspaper ash, 4.62 mg of burnt rice ash, 2.3 sugar
mg, slaked lime 1.42 mg, pine needles 1.8 mg, charcoal charcoal 1.42 mg, salt 0.64 mg, rice straw baked 0.5
2mg, Tobacco leaf grilled 0.27mg, Loess 0.27mg
And 0.18 mg of garlic are mixed and mixed with 500
After preparing a double solution, a medium in which cotton rind, corn bran, and beet were mixed at a ratio of 70:15:15 (V / V) was immersed here. Immediately after the water was absorbed into the medium, the medium was bedded and inoculated with Hiratake fungus (Shunju No. 2). The inoculated medium was put in a box and cultured at 25 ° C. for about 20 days to obtain a flat mushroom.
The cultivation method is based on a common cultivation method for flat mushrooms.

Example 2 To the non-sterile culture solution of Example 1, sawdust and rice bran were added at 75:25.
The medium mixed at a ratio of (V / V) was immersed. Immediately after the water was absorbed into the medium, the patient was bedded and inoculated with Perennial mushroom fungus (Perennial mushroom No. 1). Put the inoculated medium in a bottle and add 25
Culturing at 6 ° C. for 6 months gave perennial mushrooms. The cultivation method is based on a common cultivation method for flat mushrooms.

[0050]

According to the present invention, when cultivating mushrooms using a non-sterilized culture solution, the cultivation can be performed without sterilization, so that a separate sterilization facility (a sterilizer, a boiler, a sterilizing pressure, etc.) can be used. Without requiring a cultivation facility capable of withstanding, facility investment costs and fuel costs can be saved, and the time and manpower required for sterilization can also be saved. In addition, the method of the present invention has a lower sterilization contamination rate than the method of sterilization,
It is effective because the yield is high.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Kim Hye 210 210, Dae-ri 2-ri, Hyeongcheon-myeon, Yeoju-gun, Gyeonggi-do, Republic of Korea F-term (reference) 2B011 BA09 BA10 BA13 BA19 GA04

Claims (3)

[Claims] 1. Barley rice stone based on the total weight of the composition.
4 to 62.4% by weight, calcium 17.1 to 18.9% by weight, newspaper burnt ash 8.7 to 9.6% by weight, rice husk burnt ash 4.4 to 4.8% by weight, sugar 2.2 to 2% 2.4% by weight, slaked lime 1.3-1.5% by weight, Matsuba-yaki 1.71-1.8
9% by weight, 1.3-1.5% by weight of charcoal of kunugi, salt 0.5
8 to 0.67% by weight, rice straw baked 0.48 to 0.53% by weight, tobacco leaf baked 0.26 to 0.28% by weight, loess 0.
26-0.28% by weight and garlic 0.17-0.
A sterile culture solution composition containing 19% by weight. 2. A sterile culture solution obtained by diluting the sterile culture solution composition according to claim 1 to a concentration of 300 to 700 times,
Immersing the medium in the sterile culture solution, absorbing the sterile culture solution in the medium so that the water content is 70 to 75% by weight, and adding the medium having the sterile culture solution absorbed thereto. A method for sterilizing and cultivating mushrooms, comprising: a step of placing; a step of inoculating the medium with mushrooms; and a step of culturing the medium inoculated with the mushrooms at 20 to 25 ° C. 3. The medium is two pieces of corn bran on Nishin bell cotton.
A medium containing 0-30% (V / V), a medium containing rice bran in an amount of 20-30% (V / V), cotton rind, cotton, corn bran, and beet 80: 15: 5 (V /
V: 70:15 to 20:10 to 15 (V / V), a medium mixed in the ratio of V), or cotton rind, corn bran, and beet.
The non-sterile cultivation method according to claim 2, which is a medium mixed at a ratio of: