CN108852927A - A kind of pox-dispelling function skin care compositions and its application containing growth factor - Google Patents

A kind of pox-dispelling function skin care compositions and its application containing growth factor Download PDF

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Publication number
CN108852927A
CN108852927A CN201810658873.XA CN201810658873A CN108852927A CN 108852927 A CN108852927 A CN 108852927A CN 201810658873 A CN201810658873 A CN 201810658873A CN 108852927 A CN108852927 A CN 108852927A
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pox
skin care
care compositions
growth factor
dispelling function
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陈松彬
倪彦艳
陈健欢
郑涌镟
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Guangdong Kewei Biological Technology Co Ltd
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Guangdong Kewei Biological Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A61K8/736—Chitin; Chitosan; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

Abstract

The pox-dispelling function skin care compositions and its application that the invention discloses a kind of containing growth factor.The pox-dispelling function skin care compositions includes following components：Recombinant human epidermal growth factor, water-soluble fullerene, ArazineTM, leucojum aestivum bulb extract, bergamot extract and camellia flower extract.Rationally, proportion is scientific, and the performance anti-inflammatory of collaboration is antibacterial for each ingredient collocation of pox-dispelling function skin care compositions provided by the invention, fat secretion is adjusted, effectively dissolution grease, dredge blockage hair follicle, promote cell neogenesis and reconstruction tissue, to achieve the purpose that anti-acne, eliminate acne print.

Description

A kind of pox-dispelling function skin care compositions and its application containing growth factor
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of pox-dispelling function skin care compositions containing growth factor and It is applied.
Background technique
Whelk, also known as be acne, it is a kind of chronic inflammatory skin of pilosebaceous follicle, clinical manifestation is to be apt to occur in The pleomorphism skin lesions such as acne, papule, warts, the tubercle of face, not only have an impact to physiological health, but also psychology and society to people It hands over influence very big, and most of small poxs can all leave acne print, if permanent acne print can be left by being not treated in time, more influences beauty It sees.
Modern medicine believes that, the generation of whelk is mainly blocked with hypersteatosis, pilosebaceous duct, bacterium sense The factors such as dye and inflammatory reaction are closely related.The level of androgen especially testosterone in descendant's body that adolesces increases rapidly, Sebaceous glands is promoted to develop and generate a large amount of sebums.The dyskeratosis of pilosebaceous duct causes catheter blockage, sebum row simultaneously Obstacle out forms horn plug, that is, micro mist thorn.Multiple-microorganism especially propionibacterium acnes mass propagation, acne propionic acid in hair follicle The lipase that bacillus generates decomposes sebum and generates free fatty acid, while chemotactic inflammatory cell and medium, finally induces and aggravates inflammation Disease reaction.Whelk seriously affects the appearance and confidence of people, generates negative influence to work, the life of people, therefore anti-acne is One of research focus of cosmetics.
In order to improve the acne-removing of acne-eliminating cosmetic, researcher actively seeks various anti-acne active materials.Market at present On product for resolving poxes be primarily present following problems：1) big to skin irritation, easily cause skin allergy；2) anti-acne being added is antibacterial The normal bacterium colony of agent destruction skin；3) disabling hormone is added, causes to rely on.How the pathophysiologic features of acne-prone skin are directed to, Developing efficient and safe anti-acne class cosmetics is the direction that researcher needs to be made great efforts.103961283 A's of Publication No. CN Chinese patent application discloses a kind of anti-acne reparation lyophilized preparation, and said preparation includes LS9088, phellodendron extract, hydrolysis print banyan Cactus Flower extract, vitex agnus-castus extract, small-molecular-weight hyaluronic acid, source of people antibacterial peptide, recombination human body keratinized cell growth The factor, recombination human acidic mechanocyte growth factor, recombinant human angiopoietin-1, -1 receptor antagonist of recombination human interleukin Agent, zinc-containing metal sulfoprotein, derivative growth factor of recombined human blood platelet, liquorice root skim the cream off milk, hyaluronic acid and vitamin E Ten plurality of active ingredients such as vinegar resin acid, not only have the anti-acne of traditional raw material disappear carbuncle effect, also have effects that sterilization reparation, make the youth Acne is easier to remove and be not easy to recur again, but the cost is relatively high.
Summary of the invention
The pox-dispelling function skin care compositions and its application that the object of the present invention is to provide a kind of containing growth factor, described dispels Acne except print each ingredient of skin care compositions between synergy it is good, can effectively anti-acne, smooth acne hole, desalinate acne print, realize anti-acne effect Fruit is persistently not repeatedly.
The present invention is achieved by the following technical programs：
A kind of pox-dispelling function skin care compositions containing growth factor, comprises the following components in parts by weight：Recombinant human epidermal is raw 0.00005~0.001 part of the long factor, 3~9 parts of water-soluble fullerene, N- acetyl group-S- farnesyl--L-cysteine 0.5~3 Part, 1~4 part of leucojum aestivum bulb extract and 1~4 part of bergamot extract.
Preferably, the pox-dispelling function skin care compositions containing growth factor, further includes the component of following parts by weight：Mountain 1~6 part of Flos Camelliae Japonicae extract.
Preferably, the pox-dispelling function skin care compositions containing growth factor, comprises the following components in parts by weight：Recombination 0.0001~0.001 part of hEGF, 3~6 parts of water-soluble fullerene, N- acetyl group-S- farnesyl--L-cysteine 1~2 part, 1~3 part of leucojum aestivum bulb extract, 1~2 part of bergamot extract and 2~4 parts of camellia flower extract.
Preferably, the pox-dispelling function skin care compositions containing growth factor, comprises the following components in parts by weight：Recombination 0.0005 part of hEGF, 6 parts of water-soluble fullerene, N- acetyl group-S- farnesyl- -2 parts of L-cysteine, summer snowflake 2 parts of lotus bulb extract, 2 parts of bergamot extract and 3 parts of camellia flower extract.
Further, the water-soluble fullerene is keratan sulfate oligosaccharide-fullerene C60Compound, the water Fullerene C in dissolubility fullerene60Shared mass percent is 4~8%.
Further, the keratan sulfate oligosaccharide is in keratan sulfate disaccharides and keratan sulfate trisaccharide It is at least one.The keratan sulfate oligosaccharide can be commercial product, or made products, preparation method reference The Chinese patent application " keratan sulfate oligosaccharide fraction and the medicament containing the fraction " of Publication No. CN 1174557A, specifically For：Keratan sulfate 50g is taken, is dissolved in the 0.1M acetate buffer solution (pH6.0) of 300ml ,-β-N- acetyl group in 25U is added Glucosaminidase type sulfuric acid degrading enzyme, degrades for 24 hours at 37 DEG C.After reaction, the ethyl alcohol of 2 times of volumes is added, stirs, in room Temperature is lower to place for 1 night, and 15min is centrifuged at 4000rpm, takes supernatant (supernatant A).300ml distilled water is added into precipitating, carries out molten Solution, is added the ethyl alcohol of 3 times of amounts, and stirring placed for 1 night at room temperature, is centrifuged 15min under 4000rpm, takes supernatant (supernatant B).It will be upper Clear A and supernatant B mixing, is concentrated under reduced pressure, using Bio-Gel-P-2 column (3.6 ╳ 134cm), using distilled water as solvent, is coagulated Freeze-dried powder obtained is finally separated using ion-exchange chromatography, collects keratan sulfate respectively by glue filtering, freeze-drying The fraction of disaccharides, keratan sulfate trisaccharide, it is dry to get.
Further, the present invention also provides the preparation method of the water-soluble fullerene described in one kind, include the following steps：
(1) by keratan sulfate oligosaccharide and fullerene C60Powder is 1 by weight:20~30 are uniformly mixed, with 600~ 800r/min grinds 2~3h, and distilled water is added, is placed in constant temperature oscillator, vibrates under the conditions of 35~37 DEG C, revolving speed 100r/min 30min obtains suspension；
(2) suspension is placed in ultrasonic water bath pot, is 20~30kHz in supersonic frequency, under the conditions of temperature is 60 DEG C 60min is managed, is then filtered using 0.45 μm of filter membrane, filtrate is concentrated and dried to get water-soluble fullerene.
Pox-dispelling function skin care compositions provided by the invention containing growth factor can be used for preparing pox-dispelling function skin care item；Institute The pox-dispelling function skin care item stated are including but not limited to toner, lotion, face cream, facial mask or Essence.
In pox-dispelling function skin care compositions provided by the invention, ArazineTM(N-acetyl-S-farnesyl-L- Cysteine, N- acetyl group-S- farnesyl--L-cysteine, No. CAS：It 135304-07-3) is a kind of the anti-oxidant of anti-inflammatory Agent can be used for the anti-inflammatory to acne, and therefore, inventor is applied in anti-acne class skin care item, however, it was found that its actual use Anti-acne effect is unobvious.For this purpose, inventor is had found by largely test, with recombinant human epidermal growth factor, water-soluble fowler Alkene, ArazineTM, leucojum aestivum bulb extract and bergamot extract compounding be used as main active, play excellent dispel Acne effect, and energy conditioning skin, smooth acne hole, desalinate acne print.Wherein, water-soluble fullerene has preferable antibacterial, oil-control, soft Change cutin effect, the fat secretion that can control skin excessive peels off epidermis thanatogenic tissue, opens pore channel；Bergamot extracts Object has preferable inhibiting bacteria and diminishing inflammation effect, can effectively kill the skins parasitism flora such as propionibacterium acnes, acarid, prevents bacterium former Body etch and the long acne of inflammation；Leucojum aestivum bulb extract can suspend mode melanocyte, reduce the generation of melanin, promote pockmark Elimination, and wrinkle can be reduced, smooth skin promotes acne hole to smooth；It is micro- that recombinant human epidermal growth factor can improve skin Circulation changes the metabolism of the various cell tissues of skin, effectively restores the growth of subcutaneous connective tissue, to keep skin smooth thin Tender, acne print is eliminated in high resilience.It interacts between above each ingredient, can quickly penetrate into skin, reach and safely and efficiently dispel Acne, the purpose eliminated acne print, repair skin.Further add camellia flower extract, due to its rich in tea polyphenols and vitamin B1, B2 has the function of that significant anti-oxidant, antibacterial anti-inflammatory, regulating blood flow amount, the tea polyphenols being rich in have 5- alpha-reductase significant Inhibiting effect, be adjusted hormonal readiness, inhibit the secretion of sebaceous glands, the vitamin B1 and B2 being rich in be cell biooxidation and The coenzyme of energetic supersession, can promote development and cytothesis, can get better anti-acne, eliminates acne print effect, realizes anti-acne Effect is lasting not repeatedly.
In addition, the present inventor has found under study for action, due to keratan sulfate oligosaccharide-fullerene C60Compound introduces point Son measures relatively small keratan sulfate disaccharides and/or keratan sulfate trisaccharide, and relative to fullerene C60, keratan sulfate The content of disaccharides and/or keratan sulfate trisaccharide is higher, is used for a long time, and there is the risk for centainly causing skin saccharification, makes Obscure aging, acne print should not be eliminated, and the present invention is by a large amount of the study found that being mentioned by the way that a certain amount of leucojum aestivum bulb is added Object and bergamot extract are taken, the generation of saccharification reaction can be effectively suppressed, the bergamot extract is rich in aurantiamarin, can have Effect inhibits the generation of protein glycation dead end product；The leucojum aestivum bulb extract can enhance the activity of lysosome, promote The impaired protein of degradation, the two interaction, effectively prevent the formation of glycation end product, reduces keratan sulfate disaccharides, sulphur The skin of tamarind quality trisaccharide is saccharified risk, and the saccharification for the free sugar for inhibiting skin and containing itself, to give full play to The effect eliminated acne print, smooth acne hole.
Compared with prior art, the invention has the advantages that：
(1) rationally, proportion science, the performance of collaboration disappears each ingredient collocation of pox-dispelling function skin care compositions provided by the invention It is scorching antibacterial, fat secretion is adjusted, effectively dissolution grease, dredge blockage hair follicle promote cell neogenesis and reconstruction tissue, thus Achieve the purpose that anti-acne, eliminate pockmark.
(2) pox-dispelling function skin care compositions property provided by the invention is mild, without toxic side effect, has to skin certain Opsonic action, can be effectively suppressed the saccharification of skin, delay skin aging has preferable moistening effect and anti-oxidant effect Fruit can lighten the stain.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.Match in following embodiments The component of side is unless otherwise noted conventional commercial product, wherein hydrolysed rice albumen (Prolixir ICE) is purchased from beauty Ya Shilan group of state Ashland；Leucojum aestivum bulb extract (IBR-) it is purchased from Israel IBR company, fingered citron Mandarin orange extract and camellia flower extract are purchased from U.S. Ai Ti scientific & technical corporation (Active Concepts).
The preparation of 1 water-soluble fullerene of embodiment
(1) 10g keratan sulfate disaccharides and 500mg fullerene C are weighed60Powder is uniformly mixed, is ground with 600r/min 3h is added distilled water, is placed in constant temperature oscillator, vibrates 30min under the conditions of 35 DEG C, revolving speed 100r/min, obtain suspension；
(2) suspension is placed in ultrasonic water bath pot, is 30kHz in supersonic frequency, temperature is handled under the conditions of being 60 DEG C Then 60min is filtered using 0.45 μm of filter membrane, filtrate is concentrated and dried to get keratan sulfate disaccharides-fullerene C60 Compound, fullerene C in the compound60Shared mass percent is 8%.
The preparation of 2 water-soluble fullerene of embodiment
(1) 10g keratan sulfate trisaccharide and 200mg fullerene C are weighed60Powder is uniformly mixed, is ground with 800r/min 2h is added distilled water, is placed in constant temperature oscillator, vibrates 30min under the conditions of 37 DEG C, revolving speed 100r/min, obtain suspension；
(2) suspension is placed in ultrasonic water bath pot, is 20kHz in supersonic frequency, temperature is handled under the conditions of being 60 DEG C Then 60min is filtered using 0.45 μm of filter membrane, filtrate is concentrated and dried to get keratan sulfate trisaccharide-fullerene C60 Compound, fullerene C in the compound60Shared mass percent is 4%.
The preparation of embodiment 3-6 and comparative example 1-5 pox-dispelling function skin care compositions
The preparation of embodiment 3-6 and comparative example 1-5 pox-dispelling function skin care compositions comprise the following components in parts by weight：
Preparation method：By parts by weight shown in upper table, formula each component raw material is weighed respectively, is uniformly mixed to get anti-acne Except print skin care compositions.
It is prepared by the Whelk-eliminating paste of the 3 pox-dispelling function skin care compositions containing embodiment：
(1) it is formulated：
Surplus is deionized water.
(2) preparation method：
S1：Cetearyl alcohol alcohol ether -6, ethylhexyl palmitate, Trivent OCG, dimethyl silicone polymer are placed in oil In phase pot, 80 DEG C are heated to, is stirred to being completely dissolved, material A is obtained, is kept the temperature spare；
S2：Water, Natrulon H-10, carboxymethyl chitosan, xanthan gum are added in water phase pot, 80 DEG C is heated to, stirs It mixes to after being completely dissolved and is pumped into warmed-up emulsifying pot, open stirring, then material A is slowly pumped into emulsion pot, Cool down after matter 10min；
S3：Continue to be cooled to 35 DEG C, Sensiva SC50,3 pox-dispelling function skin care compositions of embodiment is added, stirring is equal Even, sampling Detection discharges after qualified and the Whelk-eliminating paste of the 3 pox-dispelling function skin care compositions containing embodiment is made.
The Whelk-eliminating paste preparation of the pox-dispelling function skin care compositions of 4-6 containing embodiment and comparative example 1-5 is with reference to shown in above-mentioned, respectively The additional amount of corresponding pox-dispelling function skin care compositions is 10wt% in test example.
It is prepared by the shin moisturizer of the 3 pox-dispelling function skin care compositions containing embodiment：
(1) it is formulated：
Surplus is deionized water.
(2) preparation method：3 pox-dispelling function skin care compositions of embodiment, glycerol, propylene glycol, Phenoxyethanol are added to In ionized water, stirring to material is completely dissolved, and is dispensed to get shin moisturizer.
The shin moisturizer preparation of the pox-dispelling function skin care compositions of 4-6 containing embodiment and comparative example 1-5 is with reference to shown in above-mentioned, respectively The additional amount of corresponding pox-dispelling function skin care compositions is 8wt% in test example.
Test example one, skin irritation test
It selects 180 tested volunteers as experimental subjects, is divided into 9 groups, respectively embodiment 3-6 group, comparative example 1-5 Group, every group 20, the corresponding Whelk-eliminating paste for giving 3-6 containing the embodiment of the present invention, comparative example 1-5 pox-dispelling function skin care compositions.It takes 0.1g sample is placed in the coyote hole of spot examination adhesive tape, and the spot for being equipped with sample examination adhesive tape longitudinal direction since lower part is affixed on left forearm immediately Normal skin on, while light pressing room and is uniformly distributed trier one by one to drive away air.Experiment position marks, To observe.It is primary every 24 hours patch skins, open patch half an hour after, checked according to the term of CTFA guide, altogether into 6 patch tests of row paste the 6th day after skin, in order to observe delayed response, have carried out additional inspection.Last time is examined The result looked into is compared, and standard of perfection is shown in Table 1, the results are shown in Table 2.
1 standard of perfection of table
Grade Symbol Standard of perfection
0 - Negative reaction；It is non-stimulated, without erythema
1 ± Suspicious reaction：Slight erythema
2 + Weakly positive reaction：Erythema
3 ++ Strong positive reaction：Erythema, papule, blister
4 +++ Extremely strong positive reaction：Serious edema, bulla
2 skin irritation test result of table
The results show that the Whelk-eliminating paste of 3-6 containing the embodiment of the present invention and comparative example 1-5 pox-dispelling function skin care compositions is without sun Property reaction occur, skin irritation grade be 0 grade, illustrate that pox-dispelling function skin care compositions provided by the invention is stingless to skin Swash, it is product safety, mild.
Test example two, bacteriostatic test
Referring to GB 15979-2002《Disposable Sanitary Accessory sanitary standard》" stripping property resists (suppression) bacterium to middle appendix C 4 Whether product bacteriostasis property test method " verifies the Whelk-eliminating paste containing pox-dispelling function skin care compositions provided by the invention to initiation acne The direct microorganism of acne is inhibited, the results are shown in Table 3.
3 fungistatic effect evaluation result of table
The results show that the Whelk-eliminating paste containing pox-dispelling function skin care compositions of the present invention to whelk relevant bacteria species such as：Particle third Acidfast bacilli, propionibacterium acnes, staphylococcus epidermis and staphylococcus aureus all have preferably sterilization inhibitory effect, respectively at / synergy is good, especially best with the effect of embodiment 6.
Test example three, oil-control effect test
It selects 180 tested volunteers of male as experimental subjects, is oiliness and Combination skin, without any skin disease And skin allergy history, it is divided into 9 groups, respectively embodiment 3-6 group, comparative example 1-5 group, it is every group 20, corresponding to give containing the present invention The Whelk-eliminating paste of embodiment 3-6, comparative example 1-5 pox-dispelling function skin care compositions.Tested volunteer is daily sooner or later using primary test Sample is used continuously 4 weeks.Skin oil and fat test probe (Sebumeter, Germany are being respectively adopted before and after using 4 weeks CK), SEBUMETER method is tested according to universally acknowledged oil content, the skin oil and fat content of tested volunteer is tested, tested When, probe test forehead position is tested 3 times using skin oil and fat, is averaged, experimental result is shown in Table 4.
4 oil-control effect assessment result of table
Group Before use After 4 weeks Grease change rate (%)
Embodiment 3 119.67±28.44 87.69±19.50 -26.72
Embodiment 4 122.05±32.08 91.07±20.13 -25.38
Embodiment 5 120.32±30.75 87.79±21.27 -27.04
Embodiment 6 118.80±29.26 80.45±18.62 -32.28
Comparative example 1 123.77±33.03 85.81±18.90 -30.67
Comparative example 2 120.48±32.26 96.96±19.84 -19.52
Comparative example 3 119.54±28.75 86.28±20.07 -27.82
Comparative example 4 118.26±29.08 89.84±20.53 -24.03
Comparative example 5 124.17±31.34 100.52±19.62 -19.05
The results show that skin oil and fat point can be effectively reduced using the Whelk-eliminating paste containing pox-dispelling function skin care compositions of the present invention It secretes, reaches good oil-control effect, illustrate that pox-dispelling function skin care compositions of the invention has good oil-control effect, and to implement The effect of example 6 is best.
Test example four, anti-acne effect test
Selecting 180 faces has the tested volunteer of whelk as experimental subjects, and men and women each 90, tested volunteer's Illness is divided into：1. serious：The raw acne time is long, and quantity is more, and red and swollen serious；2. slight：The raw acne time is not grown, and quantity is few, and Red and swollen not serious or without redness, in 180 tested volunteers, serious is 90, is slightly 90, respectively by severe patient It is divided into 9 groups with patients with mild, respectively embodiment 3-6 group, comparative example 1-5 group, every group of male to female ratio is consistent.Each test group is daily After face cleaning, the Whelk-eliminating paste of corresponding 3-6 containing the embodiment of the present invention, comparative example 1-5 pox-dispelling function skin care compositions are applied to face In right amount, once in the morning and once at night, observation persistently removes situation using whelk after 2 weeks.Whelk-eliminating paste is deactivated after 4 weeks, then observes whelk Recurrence.
Wherein, therapeutic effect judgment criteria is：Improve obvious：90% or more whelk is eliminated, and without acne print, acne hole is comforted Flat, skin is without other adverse reactions；Effect is general：Whelk 30~60% eliminate, and eliminate part acne print, skin without other not Good reaction；To no effect：Whelk 0~5% is eliminated, and acne print is not eliminated, and acne hole exists, and skin is without other adverse reactions, knot Fruit is shown in Table 5.
5 anti-acne test efficacy evaluation table of table
The results show that the Whelk-eliminating paste containing pox-dispelling function skin care compositions of the present invention has preferable anti-acne effect, to implement The effect of example 6 is best, and tested volunteer deactivates Whelk-eliminating paste and rebound phenomenon do not occur after 4 weeks.Show anti-acne provided by the invention Except print skin care compositions can effectively remove small pox, acne print is eliminated, smooths acne hole.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change It also should be regarded as protection scope of the present invention into retouching.

Claims (9)

1. a kind of pox-dispelling function skin care compositions containing growth factor, which is characterized in that comprise the following components in parts by weight：Recombination 0.00005~0.001 part of hEGF, 3~9 parts of water-soluble fullerene, half Guang ammonia of N- acetyl group-S- farnesyl--L- 0.5~3 part of acid, 1~4 part of leucojum aestivum bulb extract and 1~4 part of bergamot extract.
2. the pox-dispelling function skin care compositions according to claim 1 containing growth factor, which is characterized in that further include following The component of parts by weight：1~6 part of camellia flower extract.
3. the pox-dispelling function skin care compositions according to claim 2 containing growth factor, which is characterized in that including following heavy Measure the component of part：0.0001~0.001 part of recombinant human epidermal growth factor, 3~6 parts of water-soluble fullerene, N- acetyl group-S- method 1~2 part of base-L-cysteine, 1~3 part of leucojum aestivum bulb extract, 1~2 part of bergamot extract and camellia extract 2~4 parts of object.
4. the pox-dispelling function skin care compositions according to claim 3 containing growth factor, which is characterized in that including following heavy Measure the component of part：0.0005 part of recombinant human epidermal growth factor, 6 parts of water-soluble fullerene, N- acetyl group-S- farnesyl--L- half 2 parts of cystine, 2 parts of leucojum aestivum bulb extract, 2 parts of bergamot extract and 3 parts of camellia flower extract.
5. the pox-dispelling function skin care compositions according to claim 1 to 4 containing growth factor, which is characterized in that described Water-soluble fullerene be keratan sulfate oligosaccharide-fullerene C60Compound, fullerene C in the water-soluble fullerene60 Shared mass percent is 4~8%.
6. the pox-dispelling function skin care compositions according to claim 5 containing growth factor, which is characterized in that the sulfuric acid Keratan oligosaccharide is selected from least one of keratan sulfate disaccharides and keratan sulfate trisaccharide.
7. -6 any pox-dispelling function skin care compositions containing growth factor according to claim 1, which is characterized in that described The preparation of water-soluble fullerene include the following steps：
(1) by keratan sulfate oligosaccharide and fullerene C60Powder is 1 by weight:20~30 are uniformly mixed, with 600~ 800r/min grinds 2~3h, and distilled water is added, is placed in constant temperature oscillator, vibrates under the conditions of 35~37 DEG C, revolving speed 100r/min 30min obtains suspension；
(2) suspension is placed in ultrasonic water bath pot, is 20~30kHz in supersonic frequency, temperature is handled under the conditions of being 60 DEG C Then 60min is filtered using 0.45 μm of filter membrane, filtrate is concentrated and dried to get water-soluble fullerene.
8. the pox-dispelling function skin care compositions as claimed in claim 1 to 7 containing growth factor is preparing pox-dispelling function skin care item In application.
9. a kind of pox-dispelling function skin care item, which is characterized in that include the anti-acne as claimed in claim 1 to 7 containing growth factor Except print skin care compositions.
CN201810658873.XA 2018-06-25 2018-06-25 A kind of pox-dispelling function skin care compositions and its application containing growth factor Withdrawn CN108852927A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116509782A (en) * 2023-05-11 2023-08-01 百肽德医药生物科技(广东)有限公司 Acne-removing factor and cosmetics thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116509782A (en) * 2023-05-11 2023-08-01 百肽德医药生物科技(广东)有限公司 Acne-removing factor and cosmetics thereof

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