CN101283956A - Uses of choline esterase in skin nursing material for treating or improving the skin symptom - Google Patents
Uses of choline esterase in skin nursing material for treating or improving the skin symptom Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to an application of cholinesterase in preparing skin caring products for treatment or improvement of skin symptoms. The skin caring products can be applied on surface skin, hair, oral lip mucosa and genital tract surface of human body by smearing, spraying or other manners to achieve cleaning, protecting, repairing, health keeping, appearance improving, skin caring and decorating purposes. The skin caring products include, but not limited to, lyophilized power, solution, electuary, cream, emulsion, spray, water gel, and soft capsule. The cholinesterase can, after being applied on the skin of human or mammalians, deactivate P substance or other tachykinins released by skin nerve ending, inhibit skin excess immunological reaction or inflammation, relieve skin symptoms, and eliminate discomfort or trouble of a user.
Description
Technical field
The present invention relates to acetylcholine esterase in treatment or improve application in the skin care implement of skin symptom, relate to the application of acetylcholine esterase in the skin care compositions and methods of the skin symptom of antagonistic tachykinin mediation especially.
Background technology
(Tachykinins TKs) is the cluster peptide with following common amidatioon carboxyl terminal series to tachykinin.The tachykinin C-terminal has-Phe-X-Gly-Leu-Met-NH
2Common structure is the similar pharmacologically active neuropeptide of a kind of tool that produces in mammal comprises human body, and different physiological roles is arranged, and the name of tachykinin comes from their stimulations to smooth muscle tissue.The tachykinin of mammal mainly comprise the P material (Substance P, SP), neurokinin A (Neurokinin A, NKA) and neurokinin B (Neurokinin B, NKB) three kinds.Tachykinin extensively is distributed in the central nervous system peripheral nervous system of unifying, and is discharged by nerve, also can be produced by non-nervous tissue.
(SP) is example with the P material, studies show that, SP can act on various immunocytes in neuroendocrine mode, participates in immunomodulating; SP also influences the activated lymphocytes synthetic cell factor, and then mediation and the reaction of adjusting immune inflammation; SP can activate its first target cell----mastocyte, causes Ca in the born of the same parents
2+Mobilize and cell degranulation, discharge histamine.There is abundant SP binding site in application on human skin youth Ge Hansi cell surface, can suppress the angtigen presentation process after they are activated.Application on human skin injection SP causes local red and swollen, pruritus reaction; In skin injury period, SP participates in local inflammation reaction and immunoreation.
Acetylcholine esterase (Cholinesterase) is a class glycoprotein, is present in the body with multiple isozyme form.Generally can be divided into acetylcholinesterase (Acetylcholinesterase) and butyrylcholine esterase (Butylcholinesterase).Acetylcholinesterase (EC 3.1.1.7) (AChE) also claims true cholinesterase, mainly be present in assemble in the cholinergic nerve tip synaptic space, particularly the wrinkle Zhe of motorial end plate postsynaptic membrane more; Also be present in the cholinergic neuron and erythrocyte in.Butyrylcholine esterase (EC 3.1.1.8) (BChE) also claims extensively to be present in serum cholinesterase, pseudocholinesterase or non-special acetylcholine esterase in neurogliocyte, blood plasma, liver, kidney, the intestinal.
Acetylcholine esterase preparation treatment or prevention mammal comprise the people by the application in the medicine of the disease of tachykinin mediation, the applicant's Chinese patent application number is: 20061015731.8 technical literature elaborates, and document content is incorporated by reference in this text to be examined.
In existing document, do not see the report that acetylcholine esterase is used for skin care implement.
Skin is the organ of human body maximum, except having non-specific barrier function, also be an important immune organs. from broad sense, nearly all dermatosis all can be relevant with immunoreation more or less,. in skin, mastocyte is around the dermal papilla blood vessel, quantity is maximum in hair follicle, sebaceous gland and the sweat gland., its critical function is to store basophilic granule, the active medium that in these granules, contains strong pharmacological action, as histamine, leukotriene etc., the welt that skin occurs, erythema, pruritus, pain etc. are all relevant with release in the histamine release from mast cells.Big quantity research can show that the P material that is discharged by the teleneuron in the skin can cause mast cell degranulation, nerve fiber in histamine and other mastocyte products and the exogenous material chafe, cause nerve fiber to discharge neuropeptide, this influencing each other causes the extension of skin ill symptoms.
Along with the pursuit of people to U.S., the quantity of skin nursing products grows steadily.Normally, this series products of skin nursing products contains organic acid or other material as active component, for example, and some surfactant, antiseptic, spice even as protein of active substance or the like.And the various auxiliary material that come from chemical substance, have skin-irritant characteristics.The distinct disadvantage of known these active component and auxiliary material be they usually with the skin irritation of consumer or uncomfortable relevant, burn after it is characterized in that using, twinge, pruritus or tight sense.During known use local application product, significantly number of consumers has allergic skin or dermoreaction sensitivity.The product of the zest side effect that mitigation or this class active substance of elimination use cause, release market constantly, safer, environmental protection, natural, healthy product ground are released, and are that consumer is especially welcome by cosmetics or the irritated consumer of scratching that is stranded of medicine source property.In addition, the skin allergy such as Exposure to Sunlight, insect bite and pollen etc. cause also often causes discomfort or puzzlement to consumer.
Wound, burn and scald injure surgical operation, the recovery from illness process, often being accompanied by cicatrix of skin forms, especially for cicatrix gonosome matter person, if can just take out stitches or the wound healing initial stage such as perform the operation in early days, it has been carried out direct intervention, suppress the not controlled hypertrophy of cicatrix, obvious benefit should be arranged this class crowd.Medical and beauty treatment, tatoo, cosmetic surgery such as eyebrow tattooing, and microtrauma such as slight burn and scald, solar dermatitis and drug eruption etc., often cause skin injury, redness, pain even inflammation, and dermatosis and the infected by microbes even more serious than the problems referred to above, eliminate discomfort this time, improve skin symptom or improve needs attractive in appearance, rely on existing skin nursing products to be not being met far away.
Summary of the invention
The present invention seeks to, acetylcholine esterase as active substance is provided, is administered on people or the mammalian skin, teleneuron discharges in the deactivation skin P material or other tachykinin, the excessive immunoreation or the inflammation that suppress skin, the uncomfortable or puzzlement that alleviates skin symptom and elimination user.
Another object of the present invention provides the application of acetylcholine esterase in skin care implement.
A kind of acetylcholine esterase is in treatment or improve application in the skin care implement of skin symptom; described skin care implement for smear, sprinkling or additive method impose on human body or mammal surface skin, hair, lip mucosa and reproductive tract, to reach cleaning, protect, repair, keep its health, to improve its outward appearance, improve looks and be modified to the product of purpose.
Acetylcholine esterase is in treatment or improve in the application in the skin care implement of skin symptom, and described acetylcholine esterase is monomer or mixture.
Acetylcholine esterase is in treatment or improve in the application in the skin care implement of skin symptom, and described acetylcholine esterase is selected from:
Acetylcholinesterase;
Butyrylcholine esterase;
The isozyme of acetylcholinesterase or butyrylcholine esterase and derivant;
The mixture of acetylcholinesterase and butyrylcholine esterase;
The gene recombinaton thing of acetylcholinesterase or butyrylcholine esterase.
A kind of skin care implement compositions for the treatment of or improving skin symptom, acceptable skin nursing is with carrier or excipient on acetylcholine esterase that wherein contains and the Dermatology.
A kind of skin care implement compositions for the treatment of or improving skin symptom, it is characterized in that described be used for the treatment of or improve skin symptom comprise: scytitis, ulcer, rotten to the corn, macule, maculopapule, pimple, herpes, rubella, erythema, red and swollen, edema, vesicle, pustule, chickenpox, incrustation, wart, erysipelas, furuncle, furuncle, carbuncle, scabies, dermatitis, dermatosis, folliculitis, the cutin necrosis, welt, irritated, wound, burn and scald, tinea, erythra, pruritus, pain, insect stings, cicatrix, sunburn, oral ulcer, halitosis, facial yellowing, matt, lax, facial wrinkles, corse sweat pore, acne, acne, mottle, red blood streak, sunburn, scratch, skin sensitivity and medical treatment or cosmetic surgery wound.
A kind of skin care implement compositions for the treatment of or improving skin symptom, described skin symptom is relevant with infected by microbes, comprising: antibacterial, fungus, virus, actinomycetes, spirillum, chlamydia, rickettsial infection.
A kind of skin care implement compositions for the treatment of or improving skin symptom, described skin care implement compositions can be used any dosage form, includes but not limited to freeze dried powder, water preparation, unguentum, cream, Emulsion, spray, hydrogel adhesive, soft capsule.
A kind of acetylcholine esterase is in treatment or improve application in the skin care implement of skin symptom, and described acetylcholine esterase dosage range is 0.01 microgram to 1 milligram.
A kind of skin care implement compositions for the treatment of or improving skin symptom, described skin care implement compositions is preferably from cosmetics, disinfectant, medical supplies, hygienic article and daily chemicals.
The present invention is based on such theory, utilize acetylcholine esterase or its isozyme at first at C-end Leu-Met site hydrolysis amino acid residue, then multidigit point hydrolysis C-end peptide chain can give the skin symptom that improper high-level tachykinin mediates with treatment and prevention, therefore improves skin symptom.
Acetylcholine esterase is owing to source difference and means of purification difference, and it is huger than vigor difference, and every milligram of albumen does not wait to several ten thousand units from tens units, and they all can be the present invention and utilize.As to convert specific activity of enzyme be 100 units (U)/proteic dry of milligram, and the dosage range of treatment effective dose of the present invention is 0.01 microgram to 1 milligram, and the preferred dosage scope is 0.1 microgram to 1 milligram.
The invention provides acetylcholine esterase and be used for the improvement of skin care implement, a kind of effective, little means of side effect are provided problem skin.Because acetylcholine esterase of the present invention can be acetylcholinesterase or butyrylcholine esterase and their isozyme or their derivant, they can derive from cell, tissue or the blood of animal, also can derive from plant and the method by gene recombinaton obtains, wide material sources, and do not need highly purified, suitable for industrial.
The dosage form of skin care implement of the present invention has no particular limits, as long as can be administered on skin or the mucosa, any dosage form all is fine, for example, can modulator approach routinely be used to make water preparation, unguentum, cream, Emulsion etc. to the lyophilized powder of effective active composition as substrate, even can be now with the current.Also can add in existing cosmetics, disinfectant or the hygienic article and use with acetylcholine esterase as auxiliary agent or additive as active component.
The specific embodiment
Acetylcholine esterase of the present invention is in skin care implement is used, the acetylcholine esterase that uses is preferably from acetylcholinesterase, butyrylcholine esterase, the isozyme of acetylcholinesterase or butyrylcholine esterase and derivant, the mixture of acetylcholinesterase and butyrylcholine esterase, the gene recombinaton thing of acetylcholinesterase or butyrylcholine esterase.The acetylcholine esterase that uses can be a monomer, also can be mixture.The technology of their source and extraction purification, be that those skilled in the art are known, the acetylcholine esterase of separate sources, content in the raw material has nothing in common with each other, yield difference is huge, thereby the product of separate sources occurs, as the acetylcholine esterase that extracts from housefly head, electric eel, Ox blood serum and animal blood etc., utilize drosophila gene reorganization acetylcholine esterase (the open CN1472316 of referenced patent, CN1727487) etc.As mentioning a kind of preparation method that is used for the acetylcholinesterase of the fruit and vegerable pesticide determination of residual amount among the patent disclosure CN1472316, with the animal blood is the enzyme source, its processing step comprises extraction acetylcholinesterase crude extract from the enzyme source, and take off ammonium sulfate with ammonium sulfate precipitation method purification acetylcholinesterase with dialysis, again enzyme liquid is concentrated, drying promptly gets the acetylcholinesterase goods of purification.Here alleged ' purification ' is a kind of saying of custom, promptly enzyme is purified to a constant ratio and lives, and most enzyme all needs further purification.Acetylcholine esterase or its isozyme that the present invention uses only need be purified to a constant ratio and live, and are being that substrate detects under the situation with cholinesterase activity with acetylcholine or BuCh, just satisfy application of the present invention.The acetylcholine esterase that the present invention uses also can be buied from market.
Specifically, the application of acetylcholine esterase of the present invention in skin care implement be not though want to be subjected to the restriction of any theory; Described acetylcholine esterase is that the skin symptom with the mediation of tachykinin antagenists or antagonistic tachykinin works. it is characterized in that described skin care implement is: smearing, to spray or other similar approach impose on any position of human body surface { skin, hair, lip mucosa and outer reproductive tract }, with reach cleaning, protection, beauty treatment and modification, keep its health, improve its outward appearance, removal abnormal flavour and elimination microorganism be the product of purpose to its influence.Comprise cosmetics, disinfectant, medical supplies, hygienic article and health product.
Specifically, the application of acetylcholine esterase of the present invention in skin care implement can be the form of skin care compositions and methods, it is characterized in that described skin care compositions and methods wherein contains acceptable carrier or excipient on the acetylcholine esterase of effective dose and the Dermatology.
Specifically, acetylcholine esterase of the present invention can also at random combine with other components and produce the skin nursing product, these components for example are surfactant, spice, pigment, antiseptic, antioxidant, wetting agent, foaming agent, buffer agent, sunscreen, nourishing additive agent and Chinese herbal medicine or melon and fruit class etc. and plant extract, animal extracts, marine extracts, if exist, then its amount can not damaged the activity of acetylcholine esterase.
Specifically, acetylcholine esterase of the present invention can also join in existing cosmetics, disinfectant, the medical and hygiene article as adjuvant and use, and has both brought into play the effect of original product, brings into play analgesia, antiinflammatory, the antianaphylactic effect of acetylcholine esterase of the present invention again.Generally speaking, judge whether enzyme of the present invention adapts to the cosmetics or the disinfectant of routine, can determine that this is readily appreciated that to those skilled in the art according to the character of the analog of enzyme of the present invention----protease.
Specifically, compositions of the present invention is used for skin care implement, especially as being used to regulate by the inductive immunoreation of P material and suppressing purposes from the reagent of the histamine release of mastocyte.This skin care compositions and methods is applicable to processing or prevents bad skin or treatment or improve skin symptom that described bad skin or skin symptom are selected from: scytitis, ulcer, rotten to the corn, macule, maculopapule, pimple, herpes, rubella, erythema, red and swollen, edema, vesicle, pustule, chickenpox, incrustation, wart, erysipelas, furuncle, furuncle, scabies, dermatitis, dermatosis, folliculitis, the cutin necrosis, welt, irritated, wound, burn and scald, tinea, erythra, pruritus, pain, insect stings, cicatrix, sunburn, oral ulcer, halitosis, facial yellowing, matt, lax, facial wrinkles, corse sweat pore, acne, acne, mottle, red blood streak, sunburn, scratch, skin sensitivity and medical treatment or cosmetic surgery wound.
Specifically, the application of skin care compositions and methods of the present invention, be mainly used in the nursing for the treatment of or improving skin symptom and daily beauty treatment skin, and the nursing of problem skin, these problem skins can be produced by a variety of causes, as the wound of damaging, abrade, move, war wound, extreme temperature, ultraviolet, medicine source, cosmetics and disease, immunoreation, infected by microbes etc.Infected by microbes comprises: antibacterial, fungus, virus, actinomycetes, spirillum, chlamydia, rickettsial infection.
Specifically, skin care compositions and methods of the present invention can use any method to use, as smears, sprays or other similar approach impose on skin, hair, lip mucosa and outer reproductive tract. wait any position of human body surface.
Specifically, skin care compositions and methods of the present invention can be any dosage form, includes but not limited to freeze dried powder, water preparation, unguentum, cream, Emulsion, spray, hydrogel adhesive, soft capsule.In one embodiment of the invention, used freeze dried powder, be about to through filtering preliminary purification than the constant acetylcholine esterase lyophilizing of living, packing cillin bottle or ampoule bottle, it is all freezing to preserve the stage accumulating, distribution and user.Dilute with pure water during use,, use separately according to different purposes dilution different multiples.But stored refrigerated in the time of need using several times.Or add for example use such as nutrient substance, Chinese herbal medicine of other component.
Specifically, acetylcholine esterase of the present invention, its aqueous solution, also can directly or after adding excipient join acceptable carrier on the Dermatology, include but not limited to: dressing, disinfecting towel, gauze, sponge, gelfoam, sanitary towel, condom etc., be used for wound, burn and scald, burn, sunburn, decubital ulcer, chap and the nursing and the reparation of damaged skin such as medical operating, cosmetic surgery, eliminate the skin pain such as the dyspareunia that may cause in its already present pain and the preventive activities.Method be with acetylcholine esterase solution in advance or the scene join in the above-mentioned carrier.
Specifically, acetylcholine esterase of the present invention, its aqueous solution also can join in the daily chemicals and use as active component, as toothpaste, cleaning agent for mouth cavity, floral water, bath gel etc.
Now the present invention is described, should be understood that they are to be used for explanation rather than restriction the present invention with embodiment.
Embodiment
1, freeze dried powder: acetylcholine esterase enzyme liquid (enzyme activity 100U)+PEG4000 0.2% (antifreezing agent), quantitatively adorn ampoule bottle or cillin bottle.The freezing preservation of-28C.Dilute different multiples according to different purposes with pure water during use, add or do not add other auxiliary substance and directly use, or join in other skin care implements and use as auxiliary agent.
2, aqueous pharmaceutical 1: acetylcholine esterase enzyme lyophilized powder (enzyme activity 100U)+80 milliliters+PEG40010%+ of deionized water Dextran 40 5%+ mannitol 5%+ phenol 0.25+ metacresol 0.3%, packing bottle, stored refrigerated.
3, aqueous pharmaceutical 2: acetylcholine esterase enzyme lyophilized powder (enzyme activity 100U)+70 milliliters+medical glycerol of normal saline 30% (+parachlorometacresol 0.3%) packing, room temperature is preserved, and deposits six months phases.
4, soft capsule: acetylcholine esterase enzyme lyophilized powder (enzyme activity 100U)+35 milliliters+PEG400 of deionized water 30%+ hyaluronic acid 0.5% coats with gelatin glycerol capsule, and quantitatively the 0.5ml/ grain is packed with PTP outward.Room temperature is preserved.
5, skin repair: sunburn, one-level burn and scald, that skin will occur or occur will be red, bitterly, variation such as phlysis, method one applies with acetylcholine esterase soft capsule content immediately or in advance.Method two with sterile gauze or its succedaneum, immerses in the acetylcholine esterase solution with the normal saline dilution, takes out to cover on the damaged skin, can play effects such as alleviating pain, inflammation-inhibiting, elimination vesicle.Also can be used in after medical treatment or the cosmetic surgery.
6, debridement water: acetylcholine esterase freeze dried powder (enzyme activity 100U), especially is suitable for pain sensitivity person and child and war wound debridement as the cleaning wound with 1000 milliliters of dilutions of normal saline, after the war wound debridement, can carry out primary suture.Also can replace routine disinfection liquid medicine to use.
7, collutory: acetylcholine esterase freeze dried powder (enzyme activity 100U) is with 50 milliliters of dilutions of pure water, bottling, cold preservation.Cup dress water splashes into several above-mentioned collutorys when gargling, and soft drum is gargled youngster minute in the oral cavity, can alleviate oral ulcer, counteract oral malodour.
8, hydrogel adhesive: polyacrylic acid is a carbomer 0.8%, glycerol 23%, and both fully grind and make moisteningly, and it is an amount of to add water, and the allocation ratio of pressing aqueous solution 1 adds the enzyme powder, and stirring at a slow speed gets final product.Be mainly used in the skin coating.
9, the prevention of wound and inflammation thereof and treatment:
Bottled acetylcholinesterase (enzyme activity 300U) aqueous solution 1 to 3 spray of spraying is sprayed directly on to wound, and other uses with medicine and squirts the onesize gauze of wound area, cover site of injury (as need not do protection can).Patient's wound and inflammatory conditions difference, reaction has nothing in common with each other, but all obviously takes effect.As wound inflammation person not, pain disappears, not inflammation again, and wound is recovered naturally, and all dead barks afterwards begin to come off; Occurred inflammation person, pain disappears, and inflammation is no longer made progress, and after three days, recovers obviously as seen naturally.This class patient has wound, burn, the wound of scalding, burn, perform the operation.
10, tinea pedis: 2 milliliters of acetylcholinesterase (enzyme activity 300U) aqueous solutions, add the following warm water of 40 degree and put mild agitation in the basin for 2 liters, patient's foot immersed wherein 30 minutes, sooner or later respectively once, continuous three days, patient's pain promptly disappeared, and signs such as tinea speckle, abnormal smells from the patient all disappear after the week.
11, prevention dyspareunia: the soft capsule content is coated in the condom skin, or aqueous pharmaceutical 2 sprays into vagina, dyspareunia patient feels obviously to alleviate to eliminating dyspareunia, has improved life quality.
12, herpes simplex: bicker plays vesicle, pain, is coated with debridement water 1 to 2 time, and the symptom of spending the night promptly disappears.
13, prevention and treatment cicatrix:, after it is taken out stitches or removes covering, promptly spray former wound surface with debridement water if any surgical wound, burn wound, corrosion or ulcer person to Secondary cases cicatrix person may be taken place, twice of every day, a continuous week, follow up a case by regular visits to after January, do not see that clear scar generates.To outmoded cicatrix, with laser cicatrix is tied earlier, smear the soft capsule content subsequently, each once continues a week sooner or later, follows up a case by regular visits to after January, does not see new hypertrophy.
14, anti-tissue adhesion: add weight percentage ratio 0.5% hyaluronic acid in the membrane filtration debridement water, the flushing wound surface is placed wound with the suitable big or small gelfoam that fully soaks debridement water in addition, sews up then, and preventing adhesiving effect is obvious.
15, burn: a patient is burnt by pressure cooker steam outside the entire arms, immediately under the bleeder cock after a few minutes, in the position coating one time of burning, aftersensation had a position pain and 5mm left and right sides erythema occurred in 20 minutes with aqueous pharmaceutical 2, promptly apply aqueous pharmaceutical thereon, pain disappears immediately.All the other big portions have applied the position of aqueous pharmaceutical, do not see any unusual.Follow up a case by regular visits to after one week, this patient's arm outside most skin is discontinuous, irregular and darkens no any other discomfort.After first quarter moon, inform that skin color is normal.
16, gingivitis: red swelling of gingiva, pain or tenderness is arranged, soak into the debridement aqueous pharmaceutical with cotton pads or aseptic cotton balls, place the affected part, kept 5 to 10 minutes, one day three times, continuous two to three days, congestion and swelling pain all disappeared.
17, the analgesic activity of mice acetic acid twisting method is tested
(1), material and reagent
A, medicine: acetic acid, produce by chemical plant, Xinning, Guangdong; The every ml soln that makes with reference to the method among the embodiment 2 contains the butyrylcholine esterase aqueous solution of enzyme activity 200U; Ibuprofen liniment (ibuprofen) is produced batch number: 04050471 by the Sino-U.S. SmithKline.
B, animal: kunming mice, 40, body weight 20 ± 2 grams, male and female dual-purpose.
(2), method
A, grouping:
Normal saline group (negative control group);
Test 1 group, with butyrylcholine esterase aqueous solution coating
Test 2 groups, with butyrylcholine esterase aqueous solution lumbar injection
Ibuprofen group (positive controls)
B, experimental implementation:
With 40 kunming mice random packet, 10 every group; Negative control group lumbar injection 0.9% normal saline 0.1ml/10g; Test 1 group earlier in the back cropping, be cut into 1 * 1cm, and be coated with every ml soln and contain the proteinic butyrylcholine esterase aqueous solution of specific activity of enzyme 200U/ milligram; Test 2 groups of every ml solns of lumbar injection and contain the proteinic butyrylcholine esterase aqueous solution of specific activity of enzyme 200U/ milligram 0.1ml/10g; Positive controls is cut into 1 * 1cm, and coats ibuprofen earlier in the back cropping.Every group of mice sub-cage rearing, after the administration 5 minutes, with reference to " pharmacological experimental methodology " (third edition, chief editors such as Xu Shuyun, People's Health Publisher, 2002,1) Ji Zai method, every mouse peritoneal is injected 0.6% acetic acid 0.1ml/10g, writes down the number of times of mouse writhing in 15 minutes, compare with 0.9% normal saline group, and calculate mouse writhing reaction suppression ratio.
(3), result
See Table 1
Table 1
| Group | Turn round the body number of times | Suppression ratio (%) |
| The normal saline group | 37.1±6.53 | 0 |
| Test 1 group | 29.9±5.17* | 24.0 |
| Test 2 groups | 27.2±9.50*△ | 26.6 |
| The ibuprofen group | 35.7±8.40 |
Annotate: compare * P＜0.05 with the normal saline group; Compare △ P＜0.05 with the ibuprofen group.
As seen from Table 1, no matter the butyrylcholine esterase aqueous solution is that lumbar injection or skin are embrocated, with the normal saline group relatively, that all obviously reduces induced mice behind the acetic acid lumbar injection turns round body number of times (P＜0.05); Compare with positive anti-inflammation analgesis medicament ibuprofen, the butyrylcholine esterase aqueous solution also has the advantage that more significantly acts on (to test the lumbar injection group than ibuprofen group, (P＜0.05); Mouse writhing is reacted suppression ratio butyrylcholine esterase aqueous solution apparently higher than the ibuprofen group.Prompting butyrylcholine esterase aqueous solution has good analgesic activity to the acute pain reaction of mice acetic acid twisting method.
Claims (9)
1 one kinds of acetylcholine esterase are in treatment or improve application in the skin care implement of skin symptom; it is characterized in that described skin care implement for smear, sprinkling or additive method impose on human body surface skin, hair, lip mucosa and reproductive tract surface, to reach cleaning, protect, repair, keep its health, to improve its outward appearance, improve looks and be modified to the product of purpose.
2. acetylcholine esterase according to claim 1 is in treatment or improve application in the skin care implement of skin symptom, it is characterized in that described acetylcholine esterase is monomer or mixture.
3. acetylcholine esterase according to claim 1 is in treatment or improve application in the skin care implement of skin symptom, it is characterized in that described acetylcholine esterase is selected from:
Acetylcholinesterase;
Butyrylcholine esterase;
The isozyme of acetylcholinesterase or butyrylcholine esterase and derivant;
The mixture of acetylcholinesterase and butyrylcholine esterase;
The gene recombinaton thing of acetylcholinesterase or butyrylcholine esterase.
4 one kinds of skin care implement compositionss for the treatment of or improving skin symptom is characterized in that wherein containing on the acetylcholine esterase of claim 2 or 3 and the Dermatology acceptable skin nursing with carrier or excipient.
5. the described treatment of claim 4 or improve the skin care implement compositions of skin symptom, it is characterized in that described be used for the treatment of or improve skin symptom comprise: scytitis, ulcer, rotten to the corn, macule, maculopapule, pimple, herpes, rubella, erythema, red and swollen, edema, vesicle, pustule, chickenpox, incrustation, wart, erysipelas, furuncle, furuncle, carbuncle, scabies, dermatitis, dermatosis, folliculitis, the cutin necrosis, welt, irritated, wound, burn and scald, tinea, erythra, pruritus, pain, insect stings, cicatrix, sunburn, oral ulcer, halitosis, facial yellowing, matt, lax, facial wrinkles, corse sweat pore, acne, acne, mottle, red blood streak, sunburn, scratch, skin sensitivity and medical treatment or cosmetic surgery wound.
6. according to claim 4 or 5 described treatments or improve the skin care implement compositions of skin symptom, it is characterized in that described skin symptom is relevant with infected by microbes, comprising: antibacterial, fungus, virus, actinomycetes, spirillum, chlamydia, rickettsial infection.
7, treatment according to claim 4 or improve the skin care implement compositions of skin symptom is characterized in that the skin care implement compositions includes but not limited to freeze dried powder, water preparation, unguentum, cream, Emulsion, spray, hydrogel adhesive, soft capsule.
8, according to claim 1 or 2 or 3 described acetylcholine esterase in treatment or improve application in the skin care implement of skin symptom, it is characterized in that the acetylcholine esterase dosage range is 0.01 microgram to 1 milligram.
9, treatment according to claim 4 or improve the skin care implement compositions of skin symptom is characterized in that the skin care implement compositions is preferably from cosmetics, disinfectant, medical supplies, hygienic article and daily chemicals.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100666731A CN101283956A (en) | 2008-04-23 | 2008-04-23 | Uses of choline esterase in skin nursing material for treating or improving the skin symptom |
| US12/427,801 US20090263370A1 (en) | 2006-11-30 | 2009-04-22 | Methods for treating tachykinin-mediated diseases with cholinesterase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100666731A CN101283956A (en) | 2008-04-23 | 2008-04-23 | Uses of choline esterase in skin nursing material for treating or improving the skin symptom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101283956A true CN101283956A (en) | 2008-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100666731A Pending CN101283956A (en) | 2006-11-30 | 2008-04-23 | Uses of choline esterase in skin nursing material for treating or improving the skin symptom |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102127526B (en) * | 2010-01-14 | 2012-09-05 | 中国科学院微生物研究所 | Esterase and coding gene thereof |
| CN103230588A (en) * | 2013-05-10 | 2013-08-07 | 陶国良 | Application of cholinesterase capable of hydrolyzing P substance at C terminal in preparation of tachykinin antagonist |
| WO2021198684A1 (en) * | 2020-04-01 | 2021-10-07 | Neuro-Bio Ltd | Polypeptides derived from the c-terminus of acetylcholinesterase for use in skin conditions |
| WO2021198686A1 (en) * | 2020-04-01 | 2021-10-07 | Neuro-Bio Ltd | Cyclic peptides from the c-terminus of acetylcholinesterase for treatment of skin disorders and for cosmetical use |
-
2008
- 2008-04-23 CN CNA2008100666731A patent/CN101283956A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102127526B (en) * | 2010-01-14 | 2012-09-05 | 中国科学院微生物研究所 | Esterase and coding gene thereof |
| CN103230588A (en) * | 2013-05-10 | 2013-08-07 | 陶国良 | Application of cholinesterase capable of hydrolyzing P substance at C terminal in preparation of tachykinin antagonist |
| WO2021198684A1 (en) * | 2020-04-01 | 2021-10-07 | Neuro-Bio Ltd | Polypeptides derived from the c-terminus of acetylcholinesterase for use in skin conditions |
| WO2021198686A1 (en) * | 2020-04-01 | 2021-10-07 | Neuro-Bio Ltd | Cyclic peptides from the c-terminus of acetylcholinesterase for treatment of skin disorders and for cosmetical use |
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