CN108850009A - A kind of botanical pesticide and preparation method thereof of efficiently anti-locust - Google Patents
A kind of botanical pesticide and preparation method thereof of efficiently anti-locust Download PDFInfo
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- CN108850009A CN108850009A CN201810830149.0A CN201810830149A CN108850009A CN 108850009 A CN108850009 A CN 108850009A CN 201810830149 A CN201810830149 A CN 201810830149A CN 108850009 A CN108850009 A CN 108850009A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/14—Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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Abstract
The invention discloses a kind of botanical pesticides of efficiently anti-locust, are composed of the following raw materials in parts by weight：1-4 parts of triperygium wilfordii extractive,2-5 parts of euphorbia fischeriana extract,1-3 parts of sophora alopecuroides extract,1-3 parts of delphinium grandiflorum extract,180-220 parts of cosurfactant,Vegetable drug selected by the present invention is in this formula,Do not have toxic action to pest natural enemy in this ratio,It ensure that the balance of species,It is nontoxic that low toxicity is shown as to multiple beneficial biology,Non-targeted life will not be endangered,Raw material are that common vegetable drug is degradable in the environment,Noresidue,Medicinal material type is few,It is high by the effective ingredient for extracting the locust that tags,It prepares medicinal raw material and prepares the suitable industrialized production of pesticide using ultrasonic extraction,What is selected is that pure plant medicinal material does not generate drug resistance to locust,Chemical reagent in spare botanical pesticide is relatively simple convenient when transporting storage,The botanical pesticide is suitble to unmanned plane spraying operation.
Description
Technical field
The present invention relates to technical field of pesticide, the botanical pesticide and preparation method thereof of specifically a kind of efficiently anti-locust.
Background technique
Inner Mongol prairie be the maximum grassland in China and natural pasture in recent years, the whole district by EI Nino climatic effect,
Plague of locusts occurring area constantly expands.In order to guarantee farming and pastoral area crops and grassland high and stable yields, it is ensured that peasants and herdsmen's economy is not damaged
It loses, my company researchs and develops a kind of novel plant source pesticide.In addition big with chemical substances such as traditional chemical pesticide and chemical fertilizer
Amount uses, and causes harmful substance to be enriched in plant, in water, is recycled into crops and people and animals' body by substance, seriously
Agricultural product and environment are polluted, is detrimental to health, causes various diseases.This is just badly in need of a kind of efficient plant of R and D
Source pesticide carrys out the anti-plague of locusts.The medicinal plant selected in the botanical pesticide of my company's developmental research be it is degradable, to environment without dirt
Dye, the vegetable drug type of simultaneous selection is few, and insecticidal activity is relatively high, and locust will not be made to generate anti-medicine while killing locust
Property.The advantage of the invention：It is at low cost practical, it is suitble to large-scale production, the high financial profit of generation.
The locust that agriculturally crushing disaster is caused according to the thousands of years historical records in China is exactly Oedaleus asiaticus B, Asia
Oedaleus is mainly distributed on northwest drought Semiarid Grasslands of Northern in Insecta Orthoptera.And Oedaleus asiaticus B is on grassland
The probability of generation is bigger, and the chance rate or correlation maximum, locust disaster and bloods and droughts that arid occurs in plague of locusts the same year are normal
It rises one after another, alternately occurs, seriously endanger the balance of China's ecology of grassland, and the maximum grassland in China is just in the Inner Mongol
Autonomous region, this just needs us to carry out the preparation for coping with the plague of locusts in advance.
In Inner Mongolian grassland, we more go out locust the most commonly used is nosema locustae, and micro- spore goes out locust its shortcoming is that the party
Method is poor to the anti-system of advanced age locust, and micro- spore in this method is that living body preparation needs cold storage fast freight, be stored in after buying-
Under the conditions of 10 DEG C.Matching while using, manufactured poison bait will be placed on shady place, prevent solarization, and apply field as early as possible, grassland area
For wide the method there are inconvenience in terms of transport is administered storage, such method plant effect anti-to the advanced age nymph of a locust is poor.Such side
Method can also cause the hidden danger of another disaster while administering locust disaster.
Summary of the invention
The purpose of the present invention is to provide a kind of killing rate height, the plant of the efficient anti-locust of no pollution to the environment noresidue
Source pesticide and preparation method thereof, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides the following technical solutions：
A kind of botanical pesticide of efficiently anti-locust, is composed of the following raw materials in parts by weight：1-4 parts of triperygium wilfordii extractive,
2-5 parts of euphorbia fischeriana extract, 1-3 parts of sophora alopecuroides extract, 1-3 parts of delphinium grandiflorum extract, 180-220 parts of cosurfactant.
As a further solution of the present invention：
The botanical pesticide of the efficiently anti-locust, is composed of the following raw materials in parts by weight：3 parts of triperygium wilfordii extractive, wolf
3 parts of malicious root of Beijing euphorbia extract, 2 parts of sophora alopecuroides extract, 2 parts of delphinium grandiflorum extract, 200 parts of cosurfactant.
As a further solution of the present invention：
The cosurfactant is the ethyl alcohol that volume fraction is 70%.
The preparation method of the efficiently botanical pesticide of anti-locust, specific step is as follows：
(1) euphorbia fischeriana root is crushed to 95% ethyl alcohol for taking for 20-30 mesh 1 part of euphorbia fischeriana powder to add 10 parts
Being put into ultrasonic extractor and selecting power for 30 DEG C of extraction times of 500W Extracting temperature is 30 minutes, is extracted 1 time, after extraction
Solution filtering, with Rotary Evaporators temperature be 65 DEG C or so be evaporated euphorbia fischeriana medicinal extract is spare；
(2) powder 20-40 mesh is beaten by tripterygium root stem, takes 1 part of Common Threewingnut Root that 8 parts of 95% industrial alcohol is added to be put into
It is to be extracted 30 minutes at 500W temperature is 70 DEG C in power in ultrasonic extractor, the solution after extraction is filtered, is steamed with rotation
It is that 70 DEG C or so the tripterygium wilfordii medicinal extract being evaporated are spare that instrument, which is sent out, in temperature；
(3) sophora alopecuroide beats powder：Sophora alopecuroide is crushed take for 10-60 mesh 1 part of Semen Sophorae alopecuroidis powder to add 15 parts 70% by sieving
Industrial alcohol be put into ultrasonic extractor and extracted under room temperature 20 minutes in power 400W, filtering back spin inspissation is condensed to cream
Shape obtains the crude extract of Sophora alopecuroide；
(4) by delphinium grandiflorum herb dries pulverizing 50-100 mesh, 1 part of delphinium grandiflorum plant powder is taken to add 6 parts of 95% industrial wine
Essence is put into ultrasonic extractor, is to extract 20-30 minutes at 500W temperature is 60-80 DEG C in power, extracting solution is filtered back spin
Inspissation is condensed to paste, obtains the crude extract of delphinium grandiflorum；
(5) it takes triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract part to be added and helps surface
In the solution of activating agent, the botanical pesticide that mass concentration is 5% is obtained, makes to be placed at shady and cool ventilation and stores for future use.
As further scheme of the invention：
The application method of the efficiently botanical pesticide of anti-locust, is diluted with water 100 times for obtained botanical pesticide
Sprinkling.
The selection of medicinal material of the invention is according to as follows：
Euphorbia fischeriana：Euphorbiaceae euphorbia, alias euphorbia lunulata growth distribution is in the Inner Mongol, Heilungkiang, the Liao Dynasty in China
Rather, the ground such as Jilin.The well developed root system of euphorbia fischeriana is the mark of grass-land deterioration, using the growing plants on grassland by life
Produce the locust disaster on processing resistance grassland.The euphorbia fischeriana of Degraded Grasslands is replaced with simultaneously and is conducive to ecology of grassland
The plant of sustainable development.Realize economic ecology joint development.
The effective component of euphorbia fischeriana is mainly terpene, tannin class, sterol, Anthraquinones etc..Euphorbia fischeriana is wherein main
Insecticidal constituent is that the diterpenic lactone jolkinolide B content of abietane-type is higher.And jolkinolide B is small to Asia
Vehicle locust has food refusal and toxic action.And this ingredient does not generate murder by poisoning to mammal.Its manufactured vegetable insecticide activity at
It is point higher, it is easy to decompose in nature, does not generate the residue problem of pesticide while killing locust.
Tripterygium wilfordii：Also known as herba fibraureae recisae, yellow cured rattan Celastraceae tripterygium plant, it is born in the dark and damp place of mountainous region woods more, it is main to be distributed
In Zhejiang, Jiangxi, Anhui is fertile in slant acidity, the deep sand soil of soil layer or yellow soil cultivation.
The main insecticidal constituent contained in velamen of Tripterygium wilfordii is sesquiterpenoids alkaloid, and the alkaloid is to orthopteran
There is stronger food refusal toxic action.And toxicity is not generated to the herding on grassland, there is more safely good environment to environment
Compatibility and insecticidal activity.
Sophora alopecuroide originates in the Inner Mongol, Shanxi, Shaanxi, Ningxia, Gansu, Qinghai, Xinjiang, Henan, Tibet.It is born in arid more
Desert and grassland edge zone.Sophora alopecuroide is cassia leguminous plant, effective component：As aloperine in leaf content it is higher and
Do not have in root, sophocarpine and Sophoridine are then promulgated in each organ.Sophora alopecuroide total alkaloid content in Green stage leaf is
2.790%, it is 3.760% in bud, is 4.765% in stem, complete stool 5.218%；White later period leaf is 2.705%, is in stem
3.179%, 7.446% in seed, it is concentrated mainly in seed.Mainly there are 4 kinds of lifes for the anti-locust exploitation of botanical pesticide
Alkaloids：Matrine (matrineMT), oxymatrine (oxymatrinelOMT), aloperine (alopefineALO) and goldspink
Flower alkali has the insect for Orthoptera of tagging, and oxymatrine content in the seed of Sophora alopecuroide is higher.So matching in the invention
The seed of Sophora alopecuroide is selected in side, in addition Sophora alopecuroide leaf, contain various amino acid necessary to animal in seed.Leaf and lucerne
Mu compares, and other amino acid are above clover in addition to cystine；The amino acid of leaf protein is compared with milk powder, except cystine only has
Outside the half of milk powder, remaining amino acid is higher than 2 times of milk powder or so.Forage abundant is provided for the herding on grassland, we select to make
Standby botanical pesticide selection can effectively resist and go out locust but also provide forage drive economy abundant for the cattle and sheep on grassland
's.Sophora alopecuroide category mesoxerophytes, well developed root system.It is the fabulous pionner that fixes the sand again in Sandy desertification region, to dimension
The shield ecological balance, anti-desertification processed reduce soil erosion and play the role of other plant and be difficult to substitute.
Delphinium grandiflorum：For Ranunculaceae, delphinium herbaceos perennial is born in mountain valley meadow, and flower-shape is unique, exactly likes swallow one by one
Son, it is also known as delphiniums, pigeon flower.4 centimetres or so of diameter of flower, spends blue, month at florescence 7-8.Form is graceful, lovable.It is resistance to
Non-irrigated plant, heliophile.The positive, resistance to half yin, property is strong, drought-resistant, resists cold, likes Cold and cool climate, be born in hillside, meadow, fixation
Sand dune.It is mainly distributed on the ground such as Yunnan Province of China, Shanxi, Hebei, Ningxia, Sichuan, the Inner Mongol
Discovery has 29 kinds of alkaloids in delphinium grandiflorum platymiscium, wherein 21 kinds of alkaloids are with regard to insecticidal action again.According to the plant of delphinium grandiflorum
Object characteristic, selecting an ecological region planting is ornamental plant, develops the tourist industry on grassland, while playing the role of checking winds and fixing drifting sand pair
The sustainable development on grassland provides help, and it is people from grassland that after the 7-8 florescence in month, harvesting delphinium grandiflorum herb, which prepares botanical pesticide,
The economic of the people provides double-deck ensure.Contain a kind of feeding active substance in delphinium grandiflorum plant, increases locust to the formula plant agriculture
The feeding of medicine.
The active material of botanical pesticide is mainly secondary metabolites, and secondary metabolites is plant in long-term evolution
Constantly struggling against with feeding insect in the process to be not likely to produce drug resistance to insect as a result, having, low toxicity is nontoxic environmentally friendly,
It is safe and efficient, the high feature of selectivity.
The anti-locust pesticide of plant source that we prepare not only effectively kills locust, resists the plague of locusts, the danger of non agricultural chemical residuum
Evil, also provides a kind of long-term effect, while the remainder of selected medicinal material plant in the sustainable development for ecology of grassland
Forage abundant is provided for the cattle and sheep on grassland, the delphinium grandiflorum plant of selection can promote a tourist business at its florescence, be herdsman's
Income increases.It is also in friendly development trend to environment.
In summary, the root of euphorbia fischeriana, the seed of tripterygium root stem portion and Sophora alopecuroide, the preparation of delphinium grandiflorum herb are selected
The formula of botanical pesticide is a kind of practical, economic, efficient, environmentally friendly formula.
Compared with prior art, the beneficial effects of the invention are as follows：
Vegetable drug selected by the present invention in this formula, does not have toxic action to pest natural enemy in this ratio,
The balance that ensure that species, to multiple beneficial biology show as low toxicity it is nontoxic, non-targeted life will not be endangered, raw material are normal
The vegetable drug seen is degradable in the environment, noresidue, and medicinal material type is few, high by the effective ingredient for extracting the locust that tags, system
Standby medicinal raw material prepares pesticide using ultrasonic extraction and is suitble to industrialized production, and selection is that pure plant medicinal material does not produce locust
Raw drug resistance, the chemical reagent in spare botanical pesticide is relatively simple convenient when transporting storage, which is suitble to
Unmanned plane spraying operation.The benefit that the downward air-flow that unmanned plane rotor generates makes the positive aspect of plant have drug to improve drug
With rate.
Detailed description of the invention
Fig. 1 is the locust death rate schematic diagram tested in 1.
Fig. 2 is the locust death rate schematic diagram tested in 3.
Fig. 3 is the locust death rate schematic diagram tested in 4.
Fig. 4 is the locust death rate schematic diagram tested in 5.
Fig. 5 is the locust death rate schematic diagram tested in 6.
Fig. 6 is the locust death rate schematic diagram tested in 7.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Embodiment 1
A kind of botanical pesticide of efficiently anti-locust, is composed of the following raw materials in parts by weight：1 part of triperygium wilfordii extractive, wolf
2 parts of malicious root of Beijing euphorbia extract, 1 part of sophora alopecuroides extract, 1 part of delphinium grandiflorum extract, 180 parts of cosurfactant, help surface-active
Agent is the ethyl alcohol that volume fraction is 70%.
The preparation method of the efficiently botanical pesticide of anti-locust, specific step is as follows：
(1) crushing euphorbia fischeriana root is 20 mesh, takes 1 part of euphorbia fischeriana powder that 10 parts of 95% ethyl alcohol is added to put
Entering to select power in ultrasonic extractor for 30 DEG C of extraction times of 500W Extracting temperature is 30 minutes, is extracted 1 time, after extraction
Solution filtering, with Rotary Evaporators temperature be 65 DEG C or so be evaporated euphorbia fischeriana medicinal extract is spare；
(2) 20 mesh of powder is beaten by tripterygium root stem, takes 1 part of Common Threewingnut Root that 8 parts of 95% industrial alcohol is added to be put into super
It is to be extracted 30 minutes at 500W temperature is 70 DEG C in power in sound extractor, the solution after extraction is filtered, rotary evaporation is used
Instrument is that 70 DEG C or so the tripterygium wilfordii medicinal extract being evaporated are spare in temperature；
(3) sophora alopecuroide beats powder：Sieving crushes sophora alopecuroide is 10 mesh, take 1 part of Semen Sophorae alopecuroidis powder add 15 parts 70%
Industrial alcohol is put into ultrasonic extractor to be extracted 20 minutes under room temperature in power 400W, and filtering back spin inspissation is condensed to paste,
Obtain the crude extract of Sophora alopecuroide；
(4) by 50 mesh of delphinium grandiflorum herb dries pulverizing, take 1 part of delphinium grandiflorum plant powder that 6 parts of 95% industrial alcohol is added to put
Enter in ultrasonic extractor, be to be extracted 20 minutes at 500W temperature is 60 DEG C in power, extracting solution filtering back spin inspissation is condensed to cream
Shape obtains the crude extract of delphinium grandiflorum；
(5) it takes triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract part to be added and helps surface
In the solution of activating agent, the botanical pesticide that mass concentration is 5% is obtained, makes to be placed at shady and cool ventilation and stores for future use.
Embodiment 2
A kind of botanical pesticide of efficiently anti-locust, is composed of the following raw materials in parts by weight：3 parts of triperygium wilfordii extractive, wolf
3 parts of malicious root of Beijing euphorbia extract, 2 parts of sophora alopecuroides extract, 2 parts of delphinium grandiflorum extract, 200 parts of cosurfactant, help surface-active
Agent is the ethyl alcohol that volume fraction is 70%.
The preparation method of the efficiently botanical pesticide of anti-locust, specific step is as follows：
(1) crushing euphorbia fischeriana root is 25 mesh, takes 1 part of euphorbia fischeriana powder that 10 parts of 95% ethyl alcohol is added to put
Entering to select power in ultrasonic extractor for 30 DEG C of extraction times of 500W Extracting temperature is 30 minutes, is extracted 1 time, after extraction
Solution filtering, with Rotary Evaporators temperature be 65 DEG C or so be evaporated euphorbia fischeriana medicinal extract is spare；
(2) 30 mesh of powder is beaten by tripterygium root stem, takes 1 part of Common Threewingnut Root that 8 parts of 95% industrial alcohol is added to be put into super
It is to be extracted 30 minutes at 500W temperature is 70 DEG C in power in sound extractor, the solution after extraction is filtered, rotary evaporation is used
Instrument is that 70 DEG C or so the tripterygium wilfordii medicinal extract being evaporated are spare in temperature；
(3) sophora alopecuroide beats powder：Sieving crushes sophora alopecuroide is 30 mesh, take 1 part of Semen Sophorae alopecuroidis powder add 15 parts 70%
Industrial alcohol is put into ultrasonic extractor to be extracted 20 minutes under room temperature in power 400W, and filtering back spin inspissation is condensed to paste,
Obtain the crude extract of Sophora alopecuroide；
(4) by 80 mesh of delphinium grandiflorum herb dries pulverizing, take 1 part of delphinium grandiflorum plant powder that 6 parts of 95% industrial alcohol is added to put
Enter in ultrasonic extractor, is to be extracted 25 minutes at 500W temperature is 60-80 DEG C in power, concentrated by rotary evaporation after extracting solution is filtered
For paste, the crude extract of delphinium grandiflorum is obtained；
(5) it takes triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract part to be added and helps surface
In the solution of activating agent, the botanical pesticide that mass concentration is 5% is obtained, makes to be placed at shady and cool ventilation and stores for future use.
Embodiment 3
A kind of botanical pesticide of efficiently anti-locust, is composed of the following raw materials in parts by weight：4 parts of triperygium wilfordii extractive, wolf
5 parts of malicious root of Beijing euphorbia extract, 3 parts of sophora alopecuroides extract, 3 parts of delphinium grandiflorum extract, 220 parts of cosurfactant, help surface-active
Agent is the ethyl alcohol that volume fraction is 70%.
The preparation method of the efficiently botanical pesticide of anti-locust, specific step is as follows：
(1) crushing euphorbia fischeriana root is 30 mesh, takes 1 part of euphorbia fischeriana powder that 10 parts of 95% ethyl alcohol is added to put
Entering to select power in ultrasonic extractor for 30 DEG C of extraction times of 500W Extracting temperature is 30 minutes, is extracted 1 time, after extraction
Solution filtering, with Rotary Evaporators temperature be 65 DEG C or so be evaporated euphorbia fischeriana medicinal extract is spare；
(2) 40 mesh of powder is beaten by tripterygium root stem, takes 1 part of Common Threewingnut Root that 8 parts of 95% industrial alcohol is added to be put into super
It is to be extracted 30 minutes at 500W temperature is 70 DEG C in power in sound extractor, the solution after extraction is filtered, rotary evaporation is used
Instrument is that 70 DEG C or so the tripterygium wilfordii medicinal extract being evaporated are spare in temperature；
(3) sophora alopecuroide beats powder：Sieving crushes sophora alopecuroide is 60 mesh, take 1 part of Semen Sophorae alopecuroidis powder add 15 parts 70%
Industrial alcohol is put into ultrasonic extractor to be extracted 20 minutes under room temperature in power 400W, and filtering back spin inspissation is condensed to paste,
Obtain the crude extract of Sophora alopecuroide；
(4) by 100 mesh of delphinium grandiflorum herb dries pulverizing, 1 part of delphinium grandiflorum plant powder is taken to add 6 parts of 95% industrial alcohol
It is put into ultrasonic extractor, is to be extracted 30 minutes at 500W temperature is 80 DEG C in power, extracting solution filtering back spin inspissation is condensed to
Paste obtains the crude extract of delphinium grandiflorum；
(5) it takes triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract part to be added and helps surface
In the solution of activating agent, the botanical pesticide that mass concentration is 5% is obtained, makes to be placed at shady and cool ventilation and stores for future use.
After triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract are mixed plus help surface living
Property agent spray locust Plant species eaten, experimental group locust generates food refusal.And in the appearance diarrhea for being administered plant locust of searching for food, tail portion
It sticks up.Control group is all normal.
Delphinium grandiflorum extract is sprayed into locust Plant species eaten, food refusal application plant occurs in experimental group locust, and control group locust is just
Often.
3. after triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract are mixed plus helping surface
Activating agent sprays locust and plant surface, and the appearance of experimental group locust is slow in action, to environmental stimuli reactionless polypide relaxation paralysis
It is soft.Control group locust is all normal
4. after triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract are mixed plus helping surface
Activating agent sprays locust body surface and locust Plant species eaten surface, the locust of experimental group locust feeding application generate food refusal, diarrhea,
Tail portion upwarps, hind limb motor is stiff, slow in action reactionless to environmental stimuli.Control group is all normal.
5. 2-5 parts of triperygium wilfordii extractive, 2-5 parts of euphorbia fischeriana extract, 1-3 parts of sophora alopecuroides extract, delphinium grandiflorum is taken to extract
1-3 parts of object, cosurfactant is added to spray locust feeding plant and locust body surface, experimental group locust is all dead, control group locust
Worm is all normal.
6. locust vegetable drug of preferably efficiently tagging takes 3 parts of triperygium wilfordii extractive, 3 parts of euphorbia fischeriana extract, takes hardship
2 parts of bean or pea extract, take 2 parts of delphinium grandiflorum extract, be added 200ml 70% ethanol solution in dissolve, be configured to 5%
Botanical pesticide be sprayed on the plant of locust body surface and feeding, botanical pesticide is contacted by the feeding of locust, causes locust
The food refusal of worm, diarrhea, rectal prolapse, tail portion upwarp, and hind limb motor is stiff.Locust is murdered under outdoor environmental conditions indoors
Effect.
7. by 3 parts of triperygium wilfordii extractive, 3 parts of euphorbia fischeriana extract, 2 parts of sophora alopecuroides extract, 2 parts of delphinium grandiflorum extract,
At room temperature plus 70% industrial alcohol of 200ml is cosurfactant, by its configured 5% botanical pesticide water
Dilution 10-100 times, botanical pesticide dilute 10-100 times under the conditions of to indoor and outdoor surroundings under locust toxic action imitate
Fruit is obvious.
8. by 3 parts of triperygium wilfordii extractive, 3 parts of euphorbia fischeriana extract, 2 parts of sophora alopecuroides extract, 2 parts of delphinium grandiflorum extract
At room temperature, 70% industrial alcohol for adding 200ml is that cosurfactant is prepared into 5% concentration, in 5% plant source
It is same in 24 hours to 72 hours after locust feeding and contact by pesticide spraying in plant surface after 100 times of pesticide dilution
There is the locust that tags.
Specific experiment is as follows：
Experiment 1
Two groups of locusts are taken respectively, every group 50, are placed in transparent ventilated container, and by two portions of green grass of equivalent, portion impregnates real
It applies taking out for botanical pesticide 3 seconds for the preparation of example 2 and dries feeding locust, a clear water impregnates feeding locust, as a result such as the following table 1 institute
Show：
Table 1：With Four Plants extract mixed liquor to locust impact effect
By above-mentioned it is demonstrated experimentally that being extracted with sophora alopecuroides extract, triperygium wilfordii extractive, euphorbia fischeriana extract, delphinium grandiflorum
The mixed solution of composition formula has apparent killing locust effect, and has food calling, deterrent, food refusal, the work tagged to locust
With.
Experiment 2
1. taking five groups of locusts respectively, every group 50, it is placed in transparent ventilated container, by five portions of green grass of equivalent, first part clear
Water impregnates feeding locust, takes 2 parts of triperygium wilfordii extractive, 3 parts, 4 parts, the 5 parts ethanol solutions for being dissolved in 200m170% respectively,
Four portions of green grass impregnate 3 seconds feeding locusts respectively afterwards, as a result as shown in table 2 below：
Table 2：With various concentration triperygium wilfordii extractive to locust impact effect
2. taking five groups of locusts respectively, every group 50, it is placed in transparent ventilated container, by five portions of green grass of equivalent, first part clear
Water impregnate feeding locust, take respectively 2 parts of euphorbia fischeriana extract, 3 parts, 4 parts, 5 parts of ethyl alcohol for being dissolved in 200m170% it is molten
Liquid, rear four portions of green grass impregnate 3 seconds feeding locusts respectively, as a result as shown in table 3 below：
Table 3：With various concentration euphorbia fischeriana extract to locust impact effect
3. taking five groups of locusts respectively, every group 50, it is placed in transparent ventilated container, by five portions of green grass of equivalent, first part clear
Water impregnates feeding locust, takes 1 part of sophora alopecuroides extract, 2 parts, 3 parts, the 4 parts ethanol solutions for being dissolved in 200m170% respectively,
Four portions of green grass impregnate 3 seconds feeding locusts respectively afterwards, as a result as shown in table 4 below：
Table 4：With various concentration sophora alopecuroides extract to locust impact effect
4. taking five groups of locusts respectively, every group 50, it is placed in transparent ventilated container, by five portions of green grass of equivalent, first part clear
Water impregnates feeding locust, takes 1 part of delphinium grandiflorum extract, 2 parts, 3 parts, the 4 parts ethanol solutions for being dissolved in 200m170% respectively, after
Four portions of green grass impregnate 3 seconds feeding locusts respectively, as a result as shown in table 5 below：
Table 5：With various concentration delphinium grandiflorum extract to locust impact effect
Triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract deal ratio be (3: 3: 2
: 2) in the case where the ethyl alcohol of 200m170% is proportion of surfactant, insecticidal effect is preferable.
Experiment 3
The above crude extract is mixed by component ratio and is prepared into the anti-locust pesticide of 5% plant source, is placed at shady and cool ventilation and stores up
It deposits spare；
Botanical pesticide is diluted to 10 times, 50 times, 100 times respectively；Four groups of locusts are taken respectively, every group 10, are placed in
In bright ventilated container, by four portions of green grass of equivalent, 10 times of stostes of dilution is separately immersed in, 50 times of stostes is diluted, dilutes 100 times of originals
It in liquid and clear water, takes out within 3 seconds and dries feeding locust respectively, observe data, the results are shown in Table 6 for record；
Table 6：With various concentration botanical pesticide to locust impact effect
By above-mentioned experiment, it is formulated stoste and dilutes 50 times, 100 times of toxic actions to locust are it is also obvious that locust
The control of lethal dose extension rate is killed in the case where 10-100 times.
Experiment 4
Take tripterygium wilfordii crude extract, euphorbia fischeriana crude extract, Sophora alopecuroide crude extract, delphinium grandiflorum extract 200ml70% ethyl alcohol
Solution dissolution, four kinds of solution is mixed, the anti-locust stoste of 5% plant source is made.It is placed at shady and cool ventilation and stores for future use.
Two groups of locusts are taken respectively, every group 70, are placed in transparent ventilated container, and two portions of green grass of equivalent are separately immersed in
It in the anti-locust stoste of plant source and clear water, takes out within 3 seconds and dries feeding locust respectively, observe data, the results are shown in Table 7 for record；
Table 7：With the anti-locust stoste of plant source to locust impact effect
By above-mentioned experiments have shown that the delphinium grandiflorum selected in formula, Sophora alopecuroide, tripterygium wilfordii, the mixing of euphorbia fischeriana medicinal substances extract
Liquid has food calling to the locust poisoning time in 72 hours, walks quickly and keeps away, food refusal, the effect of poisoning.
Experiment 5
Take tripterygium wilfordii crude extract, euphorbia fischeriana crude extract, Sophora alopecuroide crude extract, delphinium grandiflorum extract 200ml70% ethyl alcohol
Solution dissolution, four kinds of solution is mixed, the anti-locust stoste of 5% plant source is made.It is placed at shady and cool ventilation and stores for future use.
Five groups of locusts are taken respectively, every group 50, are placed in transparent ventilated container, and five portions of green grass of equivalent are separately immersed in
The anti-locust stoste of plant source, 10 times of dilution stostes, 50 times of dilution stostes, in 100 times of dilutions stostes and clear water, taking-up in 3 seconds is dried
Feeding locust respectively, while various concentration medical fluid is sprayed on respectively in each container with locust, spray is primary within every 24 hours,
Data are observed, record result such as table 8 shows；
Table 8：With the anti-locust stoste of different diluted concentration plant sources to locust impact effect
By it is above-mentioned it is demonstrated experimentally that formula in Sophora alopecuroide, tripterygium wilfordii, euphorbia fischeriana, delphinium grandiflorum extract 5% mixing medicine
Liquid has apparent insecticidal action under 100 times of dilution, and has deterrent, food refusal, the effect tagged to locust.
Experiment 6
Take tripterygium wilfordii crude extract, euphorbia fischeriana crude extract, Sophora alopecuroide crude extract, delphinium grandiflorum extract 200ml70% ethyl alcohol
Solution dissolution, four kinds of solution is mixed, the anti-locust stoste of 5% plant source is made.It is placed at shady and cool ventilation and stores for future use.
Two groups of locusts are taken respectively, every group 100, wherein in 40 three ages, in 60 five ages, be placed in transparent ventilated container, are incited somebody to action
It dilutes 50 times of stostes and clear water is sprayed on respectively in each container with locust, (every 10 average spray values are about the left side 2.0ml
It is right), spray is primary within every 24 hours, observes data, and record result such as table 8 shows；
Table 8：50 times of solution are diluted to locust impact effect not of the same age with formula herbs crude extract
Pass through above-mentioned experiment, it can be seen that：
1, the admixing medical solutions of the Sophora alopecuroide in formula, tripterygium wilfordii, euphorbia fischeriana, delphinium grandiflorum crude extract are made with apparent desinsection
With, and there is strong poisoning to locust, it walks quickly and keeps away, the effect of food refusal.
2, it can be seen from experimental data locust spray rare death in 0 to 24 hours, death in 24 hours to 48 hours
Quantity is big, illustrates that spray value size will affect last insecticidal effect, but still have individual living bodies after 72 hours, may be due to locust
Individual difference causes, and control group is all normal.
3, botanical pesticide stoste dilutes 100 times in experiment, toxic action to locust it is also obvious that and to three ages and
Five age locusts are effective.
4, formula in Sophora alopecuroide, tripterygium wilfordii, euphorbia fischeriana, delphinium grandiflorum crude extract mixed liquor to locust have deterrent, food refusal,
The effect of poisoning.The influence of human error and locust growth characteristics and individual difference to this experiment is not excluded in experimentation.
Experiment 7
The anti-locust experiment of outdoor plant source pesticide
3 parts of tripterygium wilfordii crude extract are taken, 3 parts of euphorbia fischeriana crude extract, 2 parts of Sophora alopecuroide crude extract, delphinium grandiflorum extract part is used
The dissolution of 200ml70% ethanol solution, will be made the anti-locust stoste of 5% plant source, is placed at shady and cool ventilation and stores for future use.
2,2 pieces of the grassland of suitable locust growth, every piece of 15 square meters are chosen, and surround a closed space with green net；
3, locust 300 are taken, 150, each grassland, the locust density on grassland is 10 every square meters；
4, the solution that 100 times of dilution is uniformly sprayed in experimental group, it is primary every spray in 24 hours, it sprays five days altogether, observation
Data, record result such as table 9 show；
Table 9：With under 5% mixed solution of extract, 100 times of diluted concentrations in formula to locust impact effect on grassland
By above-mentioned experiments have shown that：
1, it is formulated the pesticide solution with 5% plant source, has in 100 times of solution of dilution to the locust on grassland and centainly refuses
Food, food calling, effect deterrent, killed, tagged.
2, the invention formula herbs have the function of apparent food refusal and certain stomach toxicity, work of tagging to locust under this proportion
With.
The description of specific exemplary embodiment of the invention is in order to illustrate the anti-locust with the formula in summary
Purpose.These descriptions are not intended to limit the invention to the precise form disclosed, and it will be apparent that according to above-mentioned guidance, can
To carry out many change and variations.The purpose of selecting and describing the exemplary embodiment is that explaining formula of the invention
Advantage and its practice, so that this technology personnel can be realized and utilize a variety of different exemplary realities of the invention
Apply scheme and various chooses and changes.
Contain this effective component of jolkinolide B in euphorbia fischeriana extract, more can increase the locust that tags of the formula
Triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract combination are to locust in the effect present invention of worm
Worm has obvious food calling stomach toxicity Antifeedant Effects, and botanical pesticide of the present invention is for orthopteran such as Oedaleus asiaticus B etc..
Above-mentioned four kinds of medicinal materials have effect of tagging to orthopteran, but it is me that above-mentioned four traditional Chinese medicine, which is not simple combination,
By many experiments conscientiously screen come.The specific manifestation formula has food refusal, stomach toxicity, action of contace poison to orthopteran.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective
Various changes can be made.
Claims (5)
1. a kind of botanical pesticide of efficiently anti-locust, which is characterized in that be composed of the following raw materials in parts by weight：Tripterygium wilfordii is extracted
1-4 parts of object, 2-5 parts of euphorbia fischeriana extract, 1-3 parts of sophora alopecuroides extract, 1-3 parts of delphinium grandiflorum extract, cosurfactant
180-220 parts.
2. the botanical pesticide of efficiently anti-locust according to claim 1, which is characterized in that by the raw material of following parts by weight
Composition：3 parts of triperygium wilfordii extractive, 2 parts of sophora alopecuroides extract, 2 parts of delphinium grandiflorum extract, helps surface living at 3 parts of euphorbia fischeriana extract
200 parts of agent of property.
3. the botanical pesticide of efficiently anti-locust according to claim 1 to 2, which is characterized in that described to help surface living
Property agent is the ethyl alcohol that volume fraction is 70%.
4. a kind of preparation method of the botanical pesticide of the efficient anti-locust as described in claim 1-2 is any, which is characterized in that
Specific step is as follows：
(1) euphorbia fischeriana root is crushed as 20-30 mesh, takes 1 part of euphorbia fischeriana powder that 10 parts of 95% ethyl alcohol is added to be put into
It for 30 DEG C of extraction times of 500W Extracting temperature is 30 minutes that power is selected in ultrasonic extractor, is extracted 1 time, by the solution after extraction
Filtering, with Rotary Evaporators temperature be 65 DEG C or so be evaporated euphorbia fischeriana medicinal extract is spare；
(2) powder 20-40 mesh is beaten by tripterygium root stem, takes 1 part of Common Threewingnut Root that 8 parts of 95% industrial alcohol is added to be put into ultrasound
It is to be extracted 30 minutes at 500W temperature is 70 DEG C in power in extractor, the solution after extraction is filtered, is existed with Rotary Evaporators
Temperature is that 70 DEG C or so the tripterygium wilfordii medicinal extract being evaporated are spare；
(3) sophora alopecuroide beats powder：Sophora alopecuroide is crushed 70% work for taking for 10-60 mesh 1 part of Semen Sophorae alopecuroidis powder to add 15 parts by sieving
Industry alcohol is put into ultrasonic extractor to be extracted 20 minutes under room temperature in power 400W, and filtering back spin inspissation is condensed to paste, is obtained
The crude extract of Sophora alopecuroide；
(4) by delphinium grandiflorum herb dries pulverizing 50-100 mesh, take 1 part of delphinium grandiflorum plant powder that 6 parts of 95% industrial alcohol is added to put
Enter in ultrasonic extractor, be to be extracted 20-30 minutes at 500W temperature is 60-80 DEG C in power, extracting solution is filtered into back spin inspissation
It is condensed to paste, obtains the crude extract of delphinium grandiflorum；
(5) it takes triperygium wilfordii extractive, euphorbia fischeriana extract, sophora alopecuroides extract, delphinium grandiflorum extract part to be added and helps surface-active
In the solution of agent, the botanical pesticide that mass concentration is 5% is obtained, makes to be placed at shady and cool ventilation and stores for future use.
5. a kind of application method of the botanical pesticide of the efficient anti-locust as described in claim 1-2 is any, which is characterized in that
Obtained botanical pesticide is diluted with water 100 times of sprinklings.
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Cited By (2)
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| CN112106789A (en) * | 2020-09-11 | 2020-12-22 | 安徽农业大学 | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth |
| CN112244042A (en) * | 2020-10-09 | 2021-01-22 | 华南农业大学 | Application of herba delphinii or extract thereof in preventing and treating sisalana ananas comosus |
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| CN112244042A (en) * | 2020-10-09 | 2021-01-22 | 华南农业大学 | Application of herba delphinii or extract thereof in preventing and treating sisalana ananas comosus |
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