CN108815089A - A kind of skin whitening, moisturizing uvioresistant peppermint toner - Google Patents

A kind of skin whitening, moisturizing uvioresistant peppermint toner Download PDF

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Publication number
CN108815089A
CN108815089A CN201811087386.9A CN201811087386A CN108815089A CN 108815089 A CN108815089 A CN 108815089A CN 201811087386 A CN201811087386 A CN 201811087386A CN 108815089 A CN108815089 A CN 108815089A
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China
Prior art keywords
uvioresistant
moisturizing
peppermint
skin
under conditions
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CN201811087386.9A
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Chinese (zh)
Inventor
陈吉祥
陶树森
李强
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Anhui Lotus Cosmetics Co Ltd
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Anhui Lotus Cosmetics Co Ltd
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Priority to CN201811087386.9A priority Critical patent/CN108815089A/en
Publication of CN108815089A publication Critical patent/CN108815089A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9755—Gymnosperms [Coniferophyta]
    • A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/82—Preparation or application process involves sonication or ultrasonication

Abstract

The invention belongs to technical field of skin care, especially a kind of skin whitening, moisturizing uvioresistant peppermint toner is prepared from the following raw materials：If mint extract, Polyphenol, broken masson pine pollen, fresh grape skin, fresh pitaya peel, wheat leaf, Taraxacum Polysaccharides, magnetized water；The mint extract, is prepared according to the following steps：Fresh mint and ethanol solution are mixed with beating, high pressure extraction processing is carried out, filtering obtains the first filtrate and filter residue；Water is added into filter residue, continues mild fire infusion after being boiled by slow fire, is ultrasonically treated, filtering obtains the second filtrate；Merge the first filtrate and the second filtrate, is spray-dried, obtains mint extract；It is naturally pollution-free not stimulate, harmful substance residual is not will cause, do not have any side effect to human body, can effectively remove free radical, to uvioresistant, inhibit tyrosinase vigor and inhibit melanin synthesis, have significant skin whitening, moisturizing uvioresistant effect.

Description

A kind of skin whitening, moisturizing uvioresistant peppermint toner
Technical field
The invention belongs to technical field of skin care, especially a kind of skin whitening, moisturizing uvioresistant peppermint toner.
Background technique
Today's society, various seriously polluted, the destruction of ecological environment makes a big impact to the skin of people, mainly includes flesh Skin flavescence, aging, the colour of skin be dimmed, serious color spot also occur in lackluster etc. or even groups of people；Although certain chemical substances pair Dispelling stain has certain curative effect, but long-time service can bring very big side effect, or even poisoning, meanwhile, these chemical substances are past It is past expensive, it is difficult to achieve the purpose that not only to beautify skin but also maintaining healthy；But as the development of social economy and people are raw The flat continuous improvement of running water, the cosmetology idea of people are increasingly turned to health cosmetic, so under the premise of ensuring safety, with certainly The right nontoxic substance with curative effect and nutrition in boundary is that the cosmetics of raw material exploitation must be by the favor of consumer；Consumer one Straight skin care item of pursuing whitening effect moisturizing and can resist injury of the ultraviolet light to skin, so inventing a kind of anti-purple of skin whitening, moisturizing The healthy toner of outside line can bring breakthrough progress for the development of skin care field.
Summary of the invention
In view of the above-mentioned problems, the present invention is intended to provide a kind of skin whitening, moisturizing uvioresistant peppermint toner.
The invention is realized by the following technical scheme：
A kind of skin whitening, moisturizing uvioresistant peppermint toner, is made of raw material from the following weight：Mint extract 0.19 ~ 0.21 Part, 0.11 ~ 0.12 part of Polyphenol, 0.17 ~ 0.18 part of broken masson pine pollen, 16.2 ~ 17.4 parts of fresh grape skin, fresh pitaya peel If 14.4 ~ 15.6 parts, 9.4 ~ 9.8 parts of wheat leaf, 0.12 ~ 0.13 part of Taraxacum Polysaccharides, 80 ~ 90 parts of magnetized water；
The mint extract, is prepared according to the following steps：By fresh mint and 1.7 ~ 1.9 times of its weight amounts, quality point Number is mixed with beating for 74% ~ 78% ethanol solution, carries out high pressure extraction processing, and filtering obtains the first filtrate and filter residue；Into filter residue The water of 2.6 ~ 2.8 times of filter residue weight amounts is added, continues 34 ~ 40min of mild fire infusion after being boiled by slow fire, is 55 ~ 57 DEG C, function in temperature Rate is ultrasonically treated 22 ~ 26min under conditions of being 200 ~ 210W, filtering obtains the second filtrate；Merge the first filtrate and the second filtrate, Spray drying, obtains mint extract.
As invention further embodiment：The magnetized water, is prepared according to the following steps：Clear water is heated to temperature It is 62 ~ 66 DEG C, 5 ~ 6min of magnetization treatment under conditions of magnetic field strength is 280 ~ 290mT is 600 ~ 610mT's in magnetic field strength Under the conditions of 3 ~ 4min of magnetization treatment, suspend 14 ~ 16min, magnetic field strength be 470 ~ 480mT under conditions of magnetization treatment 5 ~ 6min, above-mentioned is a circulation, altogether circular treatment 2 times.
As invention further embodiment：The described high pressure extraction processing, temperature be 38 ~ 40 DEG C, pressure be 100 ~ High pressure extraction handles 4 ~ 5min, high pressure under conditions of temperature is 45 ~ 47 DEG C, pressure is 185 ~ 195MPa under conditions of 110MPa 2 ~ 3min of extraction process, above-mentioned is a circulation, altogether circular treatment 2 times.
As invention further embodiment：A kind of preparation method of skin whitening, moisturizing uvioresistant peppermint toner is specific to wrap Include following steps：
（1）If fresh grape skin, fresh pitaya peel, wheat leaf and magnetized water are mixed with beating, cellulase is added and is mixed Even, merging temperature is constant-temperature enclosed 120 ~ 130h of enzymolysis processing under conditions of 34 ~ 35 DEG C, obtains leaf skin enzymatic hydrolysis slurry；
（2）Leaf skin enzymatic hydrolysis slurry is ultrasonically treated, continues 15 ~ 17min of mild fire infusion after being boiled by slow fire, hyperfiltration takes filter Mint extract, Polyphenol, broken masson pine pollen and Taraxacum Polysaccharides are added in liquid, are stirring for 360 ~ 380r/min with revolving speed It mixes device and stirs 17 ~ 19min, stir 23 ~ 25min with the blender that revolving speed is 510 ~ 530r/min, obtain skin whitening, moisturizing uvioresistant Peppermint toner.
As invention further embodiment：Step（2）The ultrasonic treatment, temperature be 58 ~ 60 DEG C, power be 192 ~ Under conditions of 198W be ultrasonically treated 3 ~ 4min, under conditions of temperature be 48 ~ 50 DEG C, power is 287 ~ 295W ultrasonic treatment 6 ~ 7min, above-mentioned is a circulation, altogether circular treatment 3 times.
Beneficial effects of the present invention：A kind of skin whitening, moisturizing uvioresistant peppermint toner provided by the invention, each raw material it Between the effect of interaction, mutually promote, preferably performance skin effect, it is natural it is pollution-free do not stimulate, not will cause nuisance Matter residual, does not have any side effect to human body, and the hydroxyl in hydrone of making up can form hydrogen bond with hydrone, has with skin Good compatibility and bonding action can be very good that moisture is promoted to enter space between cells, be easy to be absorbed by the skin, improve skin Moisture content, water lock moistening effect is lasting, also has and efficiently inhibits tyrosinase and remove the ability of free radical, promotes melanin Decomposition, increase skin itself aquation, wake up skin self-energy, reinforce the self-repairing capability of skin, pass through The enough nutrition of melanocyte is supplied, the ability of its metabolism is activated, accelerates the normal catabolism of melanin, is inhibited black The generation of pigment is not only highly resistant to the injury of ultraviolet light after in addition smearing, moreover it is possible to the hair that skin occurs after shining be effectively relieved Red hot phenomenon, farthest soothes the skin, and prevents the generation of sunburn phenomenon, promotes skin health.
Specific embodiment
Illustrate the present invention with specific embodiment below, but is not limitation of the present invention.
Embodiment 1
In the embodiment of the present invention, a kind of skin whitening, moisturizing uvioresistant peppermint toner is made of raw material from the following weight：Peppermint 0.19 part of extract, 0.11 part of Polyphenol, 0.17 part of broken masson pine pollen, 16.2 parts of fresh grape skin, fresh pitaya peel 14.4 If part, 9.4 parts of wheat leaf, 0.12 part of Taraxacum Polysaccharides, 80 parts of magnetized water；
The mint extract, is prepared according to the following steps：It is by fresh mint and 1.7 times of its weight amounts, mass fractions 74% ethanol solution is mixed with beating, and carries out high pressure extraction processing, and filtering obtains the first filtrate and filter residue；Filter residue is added into filter residue The water of 2.6 times of weight amounts continues mild fire infusion 34min after being boiled by slow fire, surpass under conditions of temperature is 55 DEG C, power is 200W Sonication 22min, filtering, obtains the second filtrate；Merge the first filtrate and the second filtrate, is spray-dried, obtains mint extract.
As invention further embodiment：The magnetized water, is prepared according to the following steps：Clear water is heated to temperature It is 62 DEG C, magnetization treatment 5min under conditions of magnetic field strength is 280mT, under conditions of magnetic field strength is 600mT at magnetization 3min is managed, 14min is suspended, magnetization treatment 5min under conditions of magnetic field strength is 470mT, above-mentioned is a circulation, is recycled altogether Processing 2 times.
As invention further embodiment：The high pressure extraction processing, in the item that temperature is 38 DEG C, pressure is 100MPa High pressure extraction handles 4min under part, and high pressure extraction handles 2 ~ 3min under conditions of temperature is 45 DEG C, pressure is 185MPa, above-mentioned It is recycled for one, altogether circular treatment 2 times.
As invention further embodiment：A kind of preparation method of skin whitening, moisturizing uvioresistant peppermint toner is specific to wrap Include following steps：
（1）If fresh grape skin, fresh pitaya peel, wheat leaf and magnetized water are mixed with beating, cellulase is added and is mixed Even, merging temperature is constant-temperature enclosed enzymolysis processing 120h under conditions of 34 DEG C, obtains leaf skin enzymatic hydrolysis slurry；
（2）Leaf skin enzymatic hydrolysis slurry is ultrasonically treated, continues mild fire infusion 15min after being boiled by slow fire, hyperfiltration takes filtrate, Mint extract, Polyphenol, broken masson pine pollen and Taraxacum Polysaccharides are added, are stirred with the blender that revolving speed is 360r/min 17min stirs 23min with the blender that revolving speed is 510r/min, obtains skin whitening, moisturizing uvioresistant peppermint toner.
As invention further embodiment：Step（2）The ultrasonic treatment, temperature is 58 DEG C, power is 192W's Under the conditions of be ultrasonically treated 3min, be ultrasonically treated 6min under conditions of temperature is 48 DEG C, power is 287W, it is above-mentioned to be one and follow Ring, total circular treatment 3 times.
Embodiment 2
In the embodiment of the present invention, a kind of skin whitening, moisturizing uvioresistant peppermint toner is made of raw material from the following weight：Peppermint 0.2 part of extract, 0.115 part of Polyphenol, 0.175 part of broken masson pine pollen, 16.8 parts of fresh grape skin, fresh pitaya peel 15 If part, 9.6 parts of wheat leaf, 0.125 part of Taraxacum Polysaccharides, 85 parts of magnetized water；
The mint extract, is prepared according to the following steps：It is by fresh mint and 1.8 times of its weight amounts, mass fractions 76% ethanol solution is mixed with beating, and carries out high pressure extraction processing, and filtering obtains the first filtrate and filter residue；Filter residue is added into filter residue The water of 2.7 times of weight amounts continues mild fire infusion 37min after being boiled by slow fire, surpass under conditions of temperature is 56 DEG C, power is 205W Sonication 24min, filtering, obtains the second filtrate；Merge the first filtrate and the second filtrate, is spray-dried, obtains mint extract.
As invention further embodiment：The magnetized water, is prepared according to the following steps：Clear water is heated to temperature It is 64 DEG C, magnetization treatment 5.5min under conditions of magnetic field strength is 285mT magnetizes under conditions of magnetic field strength is 605mT 3.5min is handled, 15min is suspended, magnetization treatment 5.5min under conditions of magnetic field strength is 475mT, above-mentioned is a circulation, Circular treatment 2 times altogether.
As invention further embodiment：The high pressure extraction processing, in the item that temperature is 39 DEG C, pressure is 105MPa High pressure extraction handles 4.5min under part, and high pressure extraction handles 2.5min under conditions of temperature is 46 DEG C, pressure is 190MPa, on It states and is recycled for one, altogether circular treatment 2 times.
As invention further embodiment：A kind of preparation method of skin whitening, moisturizing uvioresistant peppermint toner is specific to wrap Include following steps：
（1）If fresh grape skin, fresh pitaya peel, wheat leaf and magnetized water are mixed with beating, cellulase is added and is mixed Even, merging temperature is constant-temperature enclosed enzymolysis processing 125h under conditions of 34.5 DEG C, obtains leaf skin enzymatic hydrolysis slurry；
（2）Leaf skin enzymatic hydrolysis slurry is ultrasonically treated, continues mild fire infusion 16min after being boiled by slow fire, hyperfiltration takes filtrate, Mint extract, Polyphenol, broken masson pine pollen and Taraxacum Polysaccharides are added, are stirred with the blender that revolving speed is 370r/min 18min stirs 24min with the blender that revolving speed is 520r/min, obtains skin whitening, moisturizing uvioresistant peppermint toner.
As invention further embodiment：Step（2）The ultrasonic treatment, temperature is 59 DEG C, power is 195W's Under the conditions of be ultrasonically treated 3.5min, 6.5min is ultrasonically treated under conditions of temperature is 49 DEG C, power is 291W, above-mentioned is one Circulation, total circular treatment 3 times.
Embodiment 3
In the embodiment of the present invention, a kind of skin whitening, moisturizing uvioresistant peppermint toner is made of raw material from the following weight：Peppermint 0.21 part of extract, 0.12 part of Polyphenol, 0.18 part of broken masson pine pollen, 17.4 parts of fresh grape skin, fresh pitaya peel 15.6 If part, 9.8 parts of wheat leaf, 0.13 part of Taraxacum Polysaccharides, 90 parts of magnetized water；
The mint extract, is prepared according to the following steps：It is by fresh mint and 1.9 times of its weight amounts, mass fractions 78% ethanol solution is mixed with beating, and carries out high pressure extraction processing, and filtering obtains the first filtrate and filter residue；Filter residue is added into filter residue The water of 2.8 times of weight amounts continues mild fire infusion 40min after being boiled by slow fire, surpass under conditions of temperature is 57 DEG C, power is 210W Sonication 26min, filtering, obtains the second filtrate；Merge the first filtrate and the second filtrate, is spray-dried, obtains mint extract.
As invention further embodiment：The magnetized water, is prepared according to the following steps：Clear water is heated to temperature It is 66 DEG C, magnetization treatment 6min under conditions of magnetic field strength is 290mT, under conditions of magnetic field strength is 610mT at magnetization 4min is managed, 16min is suspended, magnetization treatment 6min under conditions of magnetic field strength is 480mT, above-mentioned is a circulation, is recycled altogether Processing 2 times.
As invention further embodiment：The high pressure extraction processing, in the item that temperature is 40 DEG C, pressure is 110MPa High pressure extraction handles 5min under part, and high pressure extraction handles 3min under conditions of temperature is 47 DEG C, pressure is 195MPa, above-mentioned to be One circulation, total circular treatment 2 times.
As invention further embodiment：A kind of preparation method of skin whitening, moisturizing uvioresistant peppermint toner is specific to wrap Include following steps：
（1）If fresh grape skin, fresh pitaya peel, wheat leaf and magnetized water are mixed with beating, cellulase is added and is mixed Even, merging temperature is constant-temperature enclosed enzymolysis processing 130h under conditions of 35 DEG C, obtains leaf skin enzymatic hydrolysis slurry；
（2）Leaf skin enzymatic hydrolysis slurry is ultrasonically treated, continues mild fire infusion 17min after being boiled by slow fire, hyperfiltration takes filtrate, Mint extract, Polyphenol, broken masson pine pollen and Taraxacum Polysaccharides are added, are stirred with the blender that revolving speed is 380r/min 19min stirs 25min with the blender that revolving speed is 530r/min, obtains skin whitening, moisturizing uvioresistant peppermint toner.
As invention further embodiment：Step（2）The ultrasonic treatment, temperature is 60 DEG C, power is 198W's Under the conditions of be ultrasonically treated 4min, be ultrasonically treated 7min under conditions of temperature is 50 DEG C, power is 295W, it is above-mentioned to be one and follow Ring, total circular treatment 3 times.
Comparative example 1
A kind of skin whitening, moisturizing uvioresistant peppermint toner, the difference from embodiment 1 is that, pomegranate is added without in the raw material Seed polyphenol and fresh pitaya peel, other conditions are all the same.
Comparative example 2
A kind of skin whitening, moisturizing uvioresistant peppermint toner, the difference from embodiment 1 is that, wheat is added without in the raw material If leaf and Taraxacum Polysaccharides, other conditions are all the same.
Comparative example 3
A kind of skin whitening, moisturizing uvioresistant peppermint toner, the difference from embodiment 1 is that, broken wall is added without in the raw material Pollen pini and fresh grape skin, other conditions are all the same.
Comparative example 4
A kind of skin whitening, moisturizing uvioresistant peppermint toner, the difference from embodiment 1 is that, by the magnetized water with clear water generation It replaces, other conditions are all the same.
The experiment effect of the skin whitening, moisturizing uvioresistant peppermint toner of embodiment and comparative example detects：
1. irritant experiment
It takes appropriate each group toner to be applied to volunteer (10 ~ 60 years old, 70 people, every group of 10 people) the back of the hand portion, observes each group after sixty minutes Without rubescent, macula, itch, foaming phenomena；
2. radicals scavenging effect experiment
Measuring each group toner 2mL and concentration respectively is 100 μm of ol/L diphenyl picryl phenylhydrazine (DPPH) solution 2mL, successively plus Enter and shaken up in same tool plug test tube, is allowed to stand at room temperature for 30 minutes, the absorbance of sample, inhibiting rate are measured under 517nm wavelength: K%=[1- (Ai-Aj)/Ac] × 100%, Ai is the absorbance of DPPH solution and toner, and Aj is the extinction of toner and solvent Degree, Ac are the absorbance of DPPH solution and solvent；
3. anti-ultraviolet property measures
Each group toner 2mL is measured respectively, in 280 ~ 400nm wave-length coverage, is carried out single pass every 10nm, is determined each wave The light transmittance of long lower toner, to calculate the average absorption ratio in wave-length coverage；
4. inhibiting tyrosinase viability experiment in mouse melanin cell
By B16Melanocyte is inoculated in 96 orifice plates, every 100 μ L of hole, is set and is added toner after incubator is cultivated 24 hours, and 3 Liquid is discarded supernatant after it, is rinsed twice with PBS, then every hole adds the PBS for the Triton X-100 that 90 μ L are 1% containing volume fraction, The ultrasonication in ice bath, every 10 μ L 10mmol/L levodopa of Kong Zaijia, is incubated for 60 minutes, the ratio at 475nm at 37 DEG C Color returns to zero at blank well, surveys each hole absorbance value, inhibiting rate K%=(1- medicine group mean absorbance values ÷ control group average absorbance Angle value) × 100%；
5. inhibiting mouse melanin cell melanin compound experiment
By the B from mouse16Melanocyte is with 1 × 105The density of a/mL is inoculated in culture dish, at 37 DEG C, containing 5%CO2 Under conditions of cultivate 2 days, discard supernatant liquid, add toner, abandon supernatant after 3 days, washed with PBS, 0.5mL pancreas is added in every hole Enzyme, vitellophag 3 minutes, then 2mL maintaining liquid is added in every hole and terminates digestion, after piping and druming mixes, takes out 0.5mL and make cytometer Number, remaining cell suspension are centrifuged 5 minutes under the conditions of 2500 revs/min, discard supernatant liquid, NaOH solution is added in precipitating, Heating dissolve melanin, under 490nm wavelength, using enzyme-linked immunosorbent assay instrument survey absorbance, B16 cell inhibiting rate K%= [1- (drug hole absorbance value ÷ drug hole cell density) ÷ (control wells absorbance value ÷ control wells cell density)] × 100%；
The experiment effect testing result such as table 1 of the skin whitening, moisturizing uvioresistant peppermint toner of embodiment and comparative example：
The effect of 1 embodiment and comparative example skin whitening, moisturizing uvioresistant peppermint toner of table（Unit：%）
As it can be seen from table 1 skin whitening, moisturizing uvioresistant peppermint toner of the invention is to no skin irritation, it can be effectively clear Except free radical, to uvioresistant, inhibit the vigor of tyrosinase and inhibit the synthesis of melanin, have significant skin whitening, moisturizing anti- Ultraviolet light effect, and skin whitening, moisturizing uvioresistant effect is apparently higher than commercially available skin whitening, moisturizing uvioresistant toner.

Claims (5)

1. a kind of skin whitening, moisturizing uvioresistant peppermint toner, which is characterized in that be made of raw material from the following weight：Peppermint mentions Take 0.19 ~ 0.21 part of object, 0.11 ~ 0.12 part of Polyphenol, 0.17 ~ 0.18 part of broken masson pine pollen, fresh grape skin 16.2 ~ 17.4 If part, 14.4 ~ 15.6 parts of fresh pitaya peel, 9.4 ~ 9.8 parts of wheat leaf, 0.12 ~ 0.13 part of Taraxacum Polysaccharides, magnetized water 80 ~ 90 Part；
The mint extract, is prepared according to the following steps：By fresh mint and 1.7 ~ 1.9 times of its weight amounts, quality point Number is mixed with beating for 74% ~ 78% ethanol solution, carries out high pressure extraction processing, and filtering obtains the first filtrate and filter residue；Into filter residue The water of 2.6 ~ 2.8 times of filter residue weight amounts is added, continues 34 ~ 40min of mild fire infusion after being boiled by slow fire, is 55 ~ 57 DEG C, function in temperature Rate is ultrasonically treated 22 ~ 26min under conditions of being 200 ~ 210W, filtering obtains the second filtrate；Merge the first filtrate and the second filtrate, Spray drying, obtains mint extract.
2. skin whitening, moisturizing uvioresistant peppermint toner according to claim 1, which is characterized in that the magnetized water, It is prepared according to the following steps：It is 62 ~ 66 DEG C that clear water, which is heated to temperature, magnetic under conditions of magnetic field strength is 280 ~ 290mT Change 5 ~ 6min of processing, 3 ~ 4min of magnetization treatment under conditions of magnetic field strength is 600 ~ 610mT suspends 14 ~ 16min, in magnetic field 5 ~ 6min of magnetization treatment under conditions of intensity is 470 ~ 480mT, above-mentioned is a circulation, altogether circular treatment 2 times.
3. skin whitening, moisturizing uvioresistant peppermint toner according to claim 1, which is characterized in that the high pressure extraction Processing, high pressure extraction handles 4 ~ 5min under conditions of temperature is 38 ~ 40 DEG C, pressure is 100 ~ 110MPa, is 45 ~ 47 in temperature DEG C, high pressure extraction handles 2 ~ 3min under conditions of pressure is 185 ~ 195MPa, above-mentioned is a circulation, altogether circular treatment 2 times.
4. the preparation method of described in any item skin whitening, moisturizing uvioresistant peppermint toners according to claim 1 ~ 3, feature It is, specifically includes following steps：
（1）If fresh grape skin, fresh pitaya peel, wheat leaf and magnetized water are mixed with beating, cellulase is added and is mixed Even, merging temperature is constant-temperature enclosed 120 ~ 130h of enzymolysis processing under conditions of 34 ~ 35 DEG C, obtains leaf skin enzymatic hydrolysis slurry；
（2）Leaf skin enzymatic hydrolysis slurry is ultrasonically treated, continues 15 ~ 17min of mild fire infusion after being boiled by slow fire, hyperfiltration takes filter Mint extract, Polyphenol, broken masson pine pollen and Taraxacum Polysaccharides are added in liquid, are stirring for 360 ~ 380r/min with revolving speed It mixes device and stirs 17 ~ 19min, stir 23 ~ 25min with the blender that revolving speed is 510 ~ 530r/min, obtain skin whitening, moisturizing uvioresistant Peppermint toner.
5. the preparation method of skin whitening, moisturizing uvioresistant peppermint toner according to claim 4, which is characterized in that step （2）The ultrasonic treatment is ultrasonically treated 3 ~ 4min, in temperature under conditions of temperature is 58 ~ 60 DEG C, power is 192 ~ 198W 6 ~ 7min is ultrasonically treated under conditions of being 287 ~ 295W for 48 ~ 50 DEG C, power, above-mentioned is a circulation, altogether circular treatment 3 times.
CN201811087386.9A 2018-09-18 2018-09-18 A kind of skin whitening, moisturizing uvioresistant peppermint toner Pending CN108815089A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104784063A (en) * 2015-04-07 2015-07-22 巫远程 Compound for beauty and skincare product as well as manufacturing method of the compound
CN105153099A (en) * 2015-10-17 2015-12-16 重庆都好生物科技有限公司 Method for extracting procyanidins (OPC) from pitaya peels
CN107137283A (en) * 2017-05-31 2017-09-08 南通博泰美术图案设计有限公司 A kind of toner
CN107828559A (en) * 2017-11-22 2018-03-23 浙江和也健康科技有限公司 A kind of antibacterial functions soap and preparation method thereof
CN107875092A (en) * 2017-11-28 2018-04-06 车碧萍 A kind of after-sun Essence and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104784063A (en) * 2015-04-07 2015-07-22 巫远程 Compound for beauty and skincare product as well as manufacturing method of the compound
CN105153099A (en) * 2015-10-17 2015-12-16 重庆都好生物科技有限公司 Method for extracting procyanidins (OPC) from pitaya peels
CN107137283A (en) * 2017-05-31 2017-09-08 南通博泰美术图案设计有限公司 A kind of toner
CN107828559A (en) * 2017-11-22 2018-03-23 浙江和也健康科技有限公司 A kind of antibacterial functions soap and preparation method thereof
CN107875092A (en) * 2017-11-28 2018-04-06 车碧萍 A kind of after-sun Essence and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
凰朝: "《天然面膜自制全书》", 31 October 2007, 中国纺织出版社 *

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Application publication date: 20181116