JPS63201108A - Production of water-soluble extract extracted from flower or seed of safflower - Google Patents

Abstract

PURPOSE:To obtain a water-soluble extract of safflower, by using a residue left after the extraction of pigment from tubulous flower of safflower or a residue left after the extraction of safflower oil from seed of safflower as starting raw material, hydrolyzing the material and fermenting the hydrolyzed liquid with yeast. CONSTITUTION:Residue left after the extraction of pigment from tubulous flower of safflower in flower season or a residue left after the extraction of safflower oil from seed of safflower is decomposed with an acid, alkali or enzyme and the obtained decomposed liquid is fermented with yeast to obtain safflower extract. The extract is expected to have skin-beautifying effect because of its tyrosinase-inhibiting action and melanogenesis-suppressing activity. The skin and hair can be kept from dryness and imparted with softness by the moisture-retaining property of the extract. It can be used as a drug or health food, etc., for nourishing and tonic use. The process for the production of the above extract can be applied to the extraction of crude herb drug.

Description

Detailed Description of the Invention (a) Purpose of the Invention The present invention relates to a method for producing a water-soluble extract obtained using novel safflower flowers or seeds (hereinafter simply referred to as safflower extract for convenience). It is something.

(Field of Industrial Application) The safflower extract obtained by the method of the present invention can be used in cosmetics, quasi-drugs, pharmaceuticals, drinks, etc.

Also, according to the extraction treatment method adopted in this method, it can be used to extract extracts from various medicinal plants in addition to safflower.

(Prior art) Safflower is used based on its flowers and seeds. Pigments (dyes) are extracted from the flowers, and in Chinese medicine, the residue after pressing is Since ancient times, it has been called safflower.

It has been used in pharmaceuticals (inner layer and external use), cosmetics, etc. In addition, the obtained pigments have been used as cosmetics and dyes. Furthermore, from seeds.

Safflower oil is extracted and put to effective use.

(Problems to be Solved by the Invention) Conventionally, one pigment was extracted from safflower flowers, and oil was extracted from safflower seeds. but.

The pigment extracted from the flower is called safflower, and is also called safflower, and is only used in the prescription of Chinese herbal medicine.The raw material used in the present invention is the so-called safflower flower. To obtain an H extract useful for cosmetics based on a residue obtained by removing pigments from safflower seeds and a residue obtained after extracting oil from safflower seeds.

That is, in the past, these safflower-derived residues were not particularly utilized, and their effective utilization was desired.

Therefore, the inventors remained unused. So-called,
The research and development theme was to effectively utilize the residue after extraction.

However, the extract obtained from Kouka, which has already been listed on the 1st Bureau, is obtained by drying, pulverizing, and reacting with organic solvent, enzyme, acid, or alkali.

Already developed. However, it may produce a unique off-odor (1) For example, when used in cosmetics, there has been a need for improvement measures such as removing the off-odor.

(b) 4IdL of invention Raw materials used in the present invention.

(1) Residue from extracting pigment from tubular safflower during flowering stage.

(2) Using the residue from extracting safflower oil from safflower seeds as a starting material, the decomposed liquid obtained by acid or alkali decomposition or enzymatic decomposition is further added to yeast for one fermentation. This method consists of a method for producing a safflower extract, which is characterized in that it can be obtained by

Although specifically detailed below, the A materials specified above will be simply referred to as M materials (1) and (2) below.

(Means for solving the problem) "Example!" The configuration of the invention has been described above. Raw energy derived from safflower 4 (
1) to (2), one or both of them can be hydrolyzed by adding acid or alkaline decomposition, or reacting with enzymes (cellulase, pectinase, etc.). The optimum pH in the reaction system differs depending on the combination of alkali and alkali used, or the enzyme used, but it is carried out after adjusting to the optimum pH using a conventional (known) method.) For example, as the enzymatic decomposition conditions, sufficient enzymes can be A decomposition liquid is produced.

The obtained decomposition solution is heat-treated to deactivate the enzyme if an enzyme is used, or after neutralization in the case of an acid or alkali decomposition solution, one yeast is removed. Weight (paste) per 1 decomposition solution.

Add O11~5.0% (optimal side l~2%) and add 20%
Keep at 45℃ (optimal temperature: 30-35℃), about 6-4℃.
Let fermentation take place for 8 hours. The fermented liquid obtained here is P
After adjusting the temperature to H4~F, confirming the completion of fermentation by checking the state of carbon dioxide generation and the amount of alcohol produced, the fermented liquid was decanted, centrifuged, and filtered in this order.

A clear extract-containing solution is obtained.

“Example 2” Configuration of the above-mentioned 1 invention! The rX agent derived from safflower (
Using one or both of 1) to (2), jJI! for a weight of 5 kg. 1 water 50! Contains 0 water! After stirring well and mixing, heat treat at about 80°C for 20 minutes.Next, stop heating and leave to stand for cooling.
When the temperature reaches room temperature, adjust the solution to around pH 5.0 with 99.5% acetic acid.

Next, an enzyme (for example, cellulosin A manufactured by Ueda Chemical Co., Ltd.)
C) Add 50g and stir well at about 40°C for about 18 hours to perform enzymatic decomposition, then heat at about 80°C for 20 minutes.1. The enzyme is inactivated and sterilized to complete one reaction.

The enzymatically decomposed solution obtained here was separated, left to cool at room temperature, 500 g of yeast (paste) and 2.5 kg of glucose were added, and the pH was 8.0 Nearby! ! After that, it is left to ferment for about 2 days in a thermostatic oven at about 30°C.

Next, after suction filtration using diatomaceous solution ± (Niradiolite manufactured by Showa Kagaku Kogyo), the obtained filtrate was further filtered with 0.4
Filter using a 5pm membrane filter. For the amount of filtrate obtained here.

Add methanol or ethanol to the filtrate so that the concentration is 1 Ov/V%, leave it for 1 week or more, and then refiltrate using a 0.2 pm membrane filter. A solution containing the extract in a clear state was obtained.

The respective extracts shown in Examples 1 and 2 above are:
There is little precipitation, and the extract solution containing stable water-soluble components can be stored for a long period of time. Of course, the peculiar smell is delineated.

The result is an extract solution with a very aromatic scent.

Next, when the extract according to the present invention was tested for its physicochemical properties or actions (properties, effects), the results were as shown in Tables 1 to 3.

All of them were found to be superior to extracts obtained by enzymatic digestion.

"Experimental Example 1" Looking at the tyrosinase activity inhibitory effect of the extracts obtained in Examples 1 and 2 described above and the extract obtained by acting with enzymes, the results are as shown in Table 1. It was found that the extracts obtained in Examples 1 and 2 had a higher effect of suppressing the production of melanin pigment.

That is, while safflower (Koka) is said to have a skin-whitening effect, it was also found that the residue also has this effect. In particular, it was not previously known that the residue of a single seed had this effect, and this is a new discovery.

In addition, in the test, a solution containing the extract obtained in Example 1 or 2 was diluted 10 times with water based on the freeze-dried powder (in Table 1, this was (indicated as liquid).

The enzymatically decomposed extract used for comparison was based on the decomposed solution after the enzymatic reaction was completed during the process of Example 2.

Only the yeast treatment process was cut, and the subsequent purification process was performed in the same manner as shown in Example 2, and the resultant product was freeze-dried and powdered. A solution diluted 10 times with wood (this is indicated as solution B in Table 1) was used. Further, Experimental Examples 2 and 3 were similarly carried out by 10-fold dilution.

The test method is to react tyrosine with tyrosinase and measure the melanin pigment produced at 640 nm.The composition of the reaction system is as shown below.

(Reaction composition) L-tyrosine (1.0 mg/mu) 0.5 ml phosphate buffer (PH6,8) 2.0ffi distilled water or specimen 2. OmJL copper ion (1% solution) 0.05m! Tyrosinase (1,0m
g/mJL) 1.0m Table 1 Tyrosinase activity inhibitory effect "Experimental Example 2" Regarding the thermostability, here we will discuss the heat stability of the extract obtained in Example 2 and the extract obtained by reacting with an enzyme during the process. The extract obtained without yeast treatment was tested based on the A and B solutions used in the previous test.The results are shown in Table 2, and the color tone was 50. It was found that the extract obtained by further yeast treatment after enzymatic decomposition is stable and can be stored at room temperature, except when left at 7 days at ℃.

However, in the extract obtained only by the 1111g treatment, turbidity or precipitation was observed, and the difference in quality was large, and it was found that the one-tube extraction method was superior.

Furthermore, in terms of appearance, no turbidity was observed in the extract after the yeast treatment, and furthermore, it was found to have good aromatic properties.

The color tone was measured using an absorbance meter at 4 nm.

"Experimental Example 3" Regarding the extract according to the present invention, the influence of pH was as shown in Table 3.

That is, it was found that when the pH was 9.0 or higher, slight turbidity or sludge was observed, and the color tone also showed an increasing tendency from the pH 9.0 or higher.

In particular, regarding the color tone, the extract obtained by enzymatic decomposition and the extract treated with yeast after enzymatic decomposition according to the present invention,
The same trend is shown. However, the occurrence of turbidity or sludge was found to be much lower and better in the yeast-treated extract.

The color tone was measured using an absorbance meter at 470 nm.

In addition, in Table 6 (Tables 1 to 3), liquid A and liquid B, that is, the extract after yeast treatment and the extract after enzymatic decomposition, are compared, but 1m and alkali-decomposed extracts, which have been known since ancient times, are compared. Even when compared with a hot water extract obtained using water and water, it was found that the extract after yeast treatment using water droplets was superior in stability.

m2 table -20~80℃ temperature influence 013 table P
Effect of H The following table (Table 4) shows the results of the feeling test for the lotion containing safflower extract and safflower spun extract (formulation example 1). The results showed that the safflower extract obtained by the method shown in Method 1 (Example) and the lotion containing the safflower extract had better fragrance.

There were many answers that the skin felt moisturized or had a refreshing feeling.

Table 4: Safflower extract Usage feel test results As shown above in the Examples and Experimental Examples of the present invention.

An extract containing saccharides and amino acids with excellent whitening and moisturizing effects can be obtained from safflower, and has excellent incorporation into cosmetics.

An example of its prescription is given below.

Note that extract A shown in Table 4 and in the prescription refers to the extract obtained in Example 1 and Example 2 of the present invention, and refers to an extract mainly composed of saccharides and amino acids.

(Formulation Example-1: Lotion) Extract A 3.0% Glycerin 5.0 1.3-Butylene Gelicol 4.0 Oleyl Alcohol
0.1 Polyoxysorbitan monolaurate (20E, D,) 1.5 Polyoxyethylene lauryl ether (20E, D,) 0.5 Ethyl alcohol 10.0 and 0.1 Pigment Appropriate amount Ultraviolet absorber or Preservative: Add an appropriate amount of purified water to make a total volume of 100 ml.

(Prescription example-2: cream) A extract S, O% stearin fi 14.0 Polyoxyethylene sorbitan monostearate (20E, D,) 1.5 Vaseline 2.0 Monostearin haze glycerin 2.5! , 3-butylene glycol 8.0 N
O15 antioxidant or preservative
Add an appropriate amount of purified water to make 1 too.

(Formulation example-3: Emulsion) A extract 1.0% squalane S, O petrolatum
2.O beeswax
0.5 Sorbitasesquioleic acid ester 0.8 Polyoxyethylene oleyl ether (20E, D,) 1.21.3-
Butylene gellicol 5.0 Ethyl alcohol 5.0 Carboxy vinyl polymer (1.0% water soluble) 20.0 Fragrance
Appropriate amount of antioxidant or preservative Appropriate amount of potassium hydroxide
Add 0.1 purified water to make 1 too.

Table 5: Effects of the invention of crude drugs (c) by the Japan Bureau of Medicine Traditionally, pigments have been extracted from safflower flowers! Oil was extracted from the four children. However, there has been no effective way to utilize the residue.

In other words, the residue that is removed in large quantities mainly contains starch, protein, and fiber, and based on this, the decomposition liquid obtained by hydrolysis is further processed with yeast. By fermenting it, they were able to create a fragrant extract, and they also succeeded in obtaining an extract that was mainly composed of sugars and amino acids.

Methods for producing extracts by reacting enzymes, acids, and alkalis with animal organs and medicinal plants have been known for a long time, but the present inventor has developed a method for producing extracts characterized by the action of yeast. This is the first time.

Furthermore, as shown in the experimental examples, the extract according to the present invention has a tyrosinase activity inhibiting effect and inhibits the production of melanin pigment, so it is expected to have a whitening effect. Therefore, it contains minerals that can be effectively used in cosmetics, etc.
The extract according to the present invention has a moisturizing effect and is excellent as a skin protectant, and when incorporated into cosmetics, it can prevent dryness of the skin and hair and provide flexibility. It is easy to use in liquid lotions, creams, emulsions, etc., and can also be used for nourishing and tonic purposes such as medicines and 4A floor foods.

On the other hand, if the means of Examples 1 and 2 disclosed in the present invention are adopted, it is possible to obtain extracts of not only safflower but also of plant-based crude drugs as shown in Table 5 listed in the daily cycle. It is also valid even if

Claims (1)

[Scope of Claims] [1] The starting material is a residue obtained when pigments are extracted from tubular flowers of safflower in the flowering stage, or a residue obtained when safflower oil is extracted from safflower seeds, and a known method is used. By adding yeast to the decomposition liquid obtained by hydrolysis,
A method for producing a safflower extract characterized by being obtained by fermentation.