JPH02234638A - Method for keeping freshness of plant - Google Patents

Method for keeping freshness of plant

Info

Publication number
JPH02234638A
JPH02234638A JP1055924A JP5592489A JPH02234638A JP H02234638 A JPH02234638 A JP H02234638A JP 1055924 A JP1055924 A JP 1055924A JP 5592489 A JP5592489 A JP 5592489A JP H02234638 A JPH02234638 A JP H02234638A
Authority
JP
Japan
Prior art keywords
freshness
plant
vegetables
oxidase inhibitor
polyphenol oxidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1055924A
Other languages
Japanese (ja)
Inventor
Tetsuzo Tono
東野 哲三
Shuji Fujita
修二 藤田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP1055924A priority Critical patent/JPH02234638A/en
Publication of JPH02234638A publication Critical patent/JPH02234638A/en
Pending legal-status Critical Current

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  • Storage Of Fruits Or Vegetables (AREA)

Abstract

PURPOSE:To prevent the browning of vegetables and fruits and to keep the freshness of the vegetables, etc., over a long period by using an agent containing a polyphenol oxidase inhibitor of a plant of family Cruciferae as an active component and applying the agent to the vegetables, etc., by immersion, spraying, coating, etc. CONSTITUTION:An aqueous extract liquid of a vegetable of family Cruciferae (e.g. Japanese radish, broad-leaved mustard or cabbage) is heat-treated, deproteinized e.g. with molecular sieve and subjected to enzymatic treatment with ascorbic acid to obtain a polyphenol oxidase inhibitor. The objective freshness-keeping agent for plant can be produced by using said oxidase inhibitor as an active component.

Description

【発明の詳細な説明】 (産業上の利用分野) 本発明は、浸漬〜散布、塗布などにより、野菜や果実の
鮮度を長く保持出来るようにした植物の鮮度保持剤に関
する。
DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a plant freshness-preserving agent that can maintain the freshness of vegetables and fruits for a long time by dipping, spraying, coating, etc.

(従来技術) 食品の輸送、貯蔵の過程における腐敗や鮮度の低下を防
止するため、野菜・果実の冷凍・冷蔵保存、乾燥、ある
いは輸入オレンジのように殺菌剤の直接散布、貯蔵庫や
収納器の密閉真空保存など、各種の防腐・保存方法が実
施されている。
(Prior technology) In order to prevent spoilage and deterioration of freshness during the transportation and storage process of food, vegetables and fruits must be frozen, refrigerated, dried, directly sprayed with fungicides, such as with imported oranges, or kept in storage or storage containers. Various preservative and preservation methods are used, such as sealed and vacuum storage.

鮮度保持液を用いる公知の技術として、重金属を使用す
るものと、有機物を主体とするものに大別される。前者
(特開昭61−293338,特開昭6248335)
は、安全性の点で充分とは言えず、後者(特開昭58−
5182,特公昭61−36913,特公昭62307
48,特開昭60−24152,特開昭6242693
1 .特開昭62−228223,特開昭62−257
371)は、微生物の侵入防止、ないしは殺菌効果に由
来するもので、褐変の防止効果が期待されるものとして
は、第1鉄化合物とアスコルビン酸を含む例(特公昭6
1−36913,特公昭62−30748)のみである
Known techniques using freshness-preserving liquids are broadly divided into those using heavy metals and those using organic substances as the main ingredient. The former (JP 61-293338, JP 6248335)
cannot be said to be sufficient in terms of safety;
5182, Special Publication No. 61-36913, Special Publication No. 62307
48, JP 60-24152, JP 6242693
1. JP-A-62-228223, JP-A-62-257
371) is derived from the effect of preventing the invasion of microorganisms or bactericidal effects, and examples containing ferrous compounds and ascorbic acid that are expected to have the effect of preventing browning are examples containing ferrous compounds and ascorbic acid.
1-36913, Special Publication No. 62-30748).

(発明が解決しようとする課題) 従来技術は、食品衛生法上の毒性や、保冷設備などの経
費の点で一長一短があり、そのため手軽に実施出来て、
かつ野菜、果実に用いても安全な鮮度保持法の開発が望
まれている。
(Problems to be solved by the invention) Conventional techniques have advantages and disadvantages in terms of toxicity under the Food Sanitation Law and expenses for cold storage equipment, etc. Therefore, it is easy to implement,
There is also a desire to develop a method for preserving freshness that is safe for use on vegetables and fruits.

(課題を解決するための手段) 本発明者らは、新しい褐変防止・鮮度保持剤を開発する
ため、野菜やフルーツの褐変に関与するポリフェノール
酸化酵素の阻害活性を指標に、アブラナ科の野菜の抽出
液を中心にスクリーニングを行ったところ、ダイコン,
タカナ,凌キャベツ冫などの抽出液に阻害活性を認めた
。これらの抽出液に浸消した野菜・果実さらにはカソト
野菜等では、褐変が起らず鮮度が長く保持されることを
見出し、本発明を完成した。
(Means for Solving the Problem) In order to develop a new anti-browning/freshness-preserving agent, the present inventors used the inhibitory activity of polyphenol oxidase, which is involved in the browning of vegetables and fruits, as an indicator. When we screened mainly extracts, we found that radish,
Inhibitory activity was observed in the extracts of Takana and Ling cabbage. We have completed the present invention by discovering that vegetables, fruits, and even Kasotho vegetables that have been soaked in these extracts do not brown and retain their freshness for a long time.

即ち本発明は、アブラナ科の野菜の中から選ばれた少な
くとも1種の水抽出液からなる、野菜・果実の褐変防止
・鮮度保持剤を提供するものである。
That is, the present invention provides an agent for preventing browning and preserving freshness of vegetables and fruits, which is made of an aqueous extract of at least one kind selected from vegetables of the cruciferous family.

本発明で用いるアブラナ科植物は、ダイコン,タカナ,
キャベツなどの植物であり、本発明で用いるポリフェノ
ール酸化酵素阻害剤は、これらの植物の少なくとも1種
の抽出物を除蛋白したもののなかに存在するものである
。除蛋白の方法は、特に限定されたものでは無く、通常
用いる方法で良く、例えば加熱処理、分子ふるい等で処
理すれば良い。
The cruciferous plants used in the present invention include radish, takana,
The polyphenol oxidase inhibitor used in the present invention is a plant such as cabbage, and the polyphenol oxidase inhibitor used in the present invention is present in a deproteinized extract of at least one of these plants. The protein removal method is not particularly limited and may be any commonly used method, such as heat treatment, molecular sieves, etc.

本発明で用いるポリフェノール酸化酵素阻害剤は、アス
コルビン酸酸化酵素で処理されたものの中に存在する。
The polyphenol oxidase inhibitor used in the present invention is present in those treated with ascorbic acid oxidase.

この酵素処理は、植物抽出液を除蛋白した後でも前でも
良い。酵素処理条件は特に限定された条件を必要とせず
、酵素が活性を発現する条件であれば良い。本酵素によ
り、抽出物中に共存するアスコルビン酸は分解されるた
め、本抽出物中のポリフェノール酸化酵素阻害剤は、ア
スコルビン酸とは異なるものである。更に本発明に用い
るポリフェノール酸化酵素阻害剤は、少なくとも100
℃,60分間の加熱で阻害活性の低下を示さないもので
ある。
This enzyme treatment may be performed after or before deproteinizing the plant extract. Enzyme treatment conditions do not need to be particularly limited, as long as they allow the enzyme to express its activity. Since ascorbic acid coexisting in the extract is degraded by this enzyme, the polyphenol oxidase inhibitor in this extract is different from ascorbic acid. Furthermore, the polyphenol oxidase inhibitor used in the present invention has at least 100
C. and shows no decrease in inhibitory activity after heating for 60 minutes.

本発明に用いるポリフェノール酸化酵素阻害剤の、基質
にクロロゲン酸を用いた場合の阻害様式は、図1に示す
通り拮抗阻害型である。
The inhibition mode of the polyphenol oxidase inhibitor used in the present invention when chlorogenic acid is used as a substrate is a competitive inhibition type as shown in FIG.

本発明において、野菜・果実に対する鮮度保持剤の適用
量は特に制限はなく、対象野菜・果実、望まれる保存期
間などに応じて適宜定めることが出来る。
In the present invention, the amount of the freshness-preserving agent applied to vegetables and fruits is not particularly limited, and can be determined as appropriate depending on the target vegetables and fruits, the desired storage period, and the like.

本発明の褐変防止・鮮度保持剤は、通常、野菜・果実に
塗布・散布したり、野菜・果実を浸清したりして用いら
れるが、適用方法には特に制限はなく、どのようにして
用いてもよい。
The anti-browning/freshness-preserving agent of the present invention is usually used by applying or spraying on vegetables and fruits, or by soaking vegetables and fruits, but there are no particular restrictions on the method of application. May be used.

本発明によれば、野菜・果実の褐変を防止して、鮮度を
長く保持することができるという優れた効果を奏する。
According to the present invention, it is possible to prevent browning of vegetables and fruits and maintain their freshness for a long time, which is an excellent effect.

又、有効成分は、食用に供されるアブラナ科野菜からの
抽出物であり、従来の殺菌剤、重金属等は一切使用せず
人畜に対して安全な成分である。従って、産地から市場
までの流通段階においてばかりでなく、消費者まで通し
た一貫した鮮度保持ができる点で特に有利である。
In addition, the active ingredient is an extract from edible cruciferous vegetables, and does not contain any conventional fungicides or heavy metals, making it safe for humans and animals. Therefore, it is particularly advantageous in that freshness can be consistently maintained not only at the distribution stage from the production area to the market, but also throughout the process up to the consumer.

次に本発明を実施例に基づき更に詳細に説明する。Next, the present invention will be explained in more detail based on examples.

実施例 (A)鮮度保持剤の調製 (B)鮮度保持試験 A 鮮度保持剤の活性表示 ポリフェノール酸化酵素のクロロゲン酸酸化活性の阻害
の程度により、次のように表わすことと?クロロゲン酸
酸化反応を、下記の条件でサンプルの添加、無添加(コ
ントロール)で進行させ、初期5分間の褐変度(ΔA4
■。)を測定し、次式により阻害率を算出し、阻害率1
%をlunit表示することとした。
Example (A) Preparation of freshness preservation agent (B) Freshness preservation test A Indication of activity of freshness preservation agent Depending on the degree of inhibition of chlorogenic acid oxidation activity of polyphenol oxidase, it is expressed as follows. The chlorogenic acid oxidation reaction was allowed to proceed under the following conditions with and without the addition of a sample (control), and the degree of browning (ΔA4) during the initial 5 minutes was
■. ) was measured, and the inhibition rate was calculated using the following formula, and the inhibition rate 1
% was expressed in lunit.

ポリフェノール酸化酵素(PPO)の測定条件鮮度保持
剤の調製法(ダイコン) ポリフェノール酸化酵素のクロロゲン酸酸化活性の阻害
を指標Cこ、約5kgのダイコンをオロシガ不で破砕し
、綿布で絞った後ち、その絞り汁を遠心分離し(700
0g, 20min) 、上澄液を得た。これを沸騰湯
浴」二で60分間加熱し、蛋白を変性させた後吸引ろ過
でこれを除去した。このろ過液を凍結乾燥により濃縮し
、50QmI!.の濃縮液を得た。
Measurement conditions for polyphenol oxidase (PPO) Preparation method of freshness preservation agent (radish) Inhibition of chlorogenic acid oxidation activity of polyphenol oxidase is the indicator C. After crushing approximately 5 kg of radish with Oroshigafu and squeezing it with cotton cloth. , centrifuge the squeezed juice (700
0 g, 20 min) to obtain a supernatant. This was heated in a boiling water bath for 60 minutes to denature the protein, which was then removed by suction filtration. This filtrate was concentrated by freeze-drying to 50QmI! .. A concentrated solution was obtained.

次にこの濃縮液5 mj2を用いで、Sephadex
 G −25によるゲルろ過を行った(カラムサイズ1
,5×40cm,分画液M5ml)。阻害活性の検出さ
れた分画本数21から23本目の活性画分を集め鮮度保
持剤として用いた(この時の鮮度保持活性4000un
it/m 1 )。
Next, using 5 mj2 of this concentrated solution, Sephadex
Gel filtration was performed using G-25 (column size 1
, 5 x 40 cm, fraction solution M5 ml). The active fractions from the 21st to 23rd fractions in which inhibitory activity was detected were collected and used as a freshness-preserving agent (at this time, the freshness-preserving activity was 4000 un).
it/m 1 ).

以下の試験には、この試料を用いた。This sample was used in the following tests.

鮮度保持試験 上記テ調製した鮮度保持剤(4000uni t’ s
/m !l )を用いて、ゴボウとレタスの鮮度保持効
果を調べた。ゴボウの根の横断面と、レタス茎基部横断
面をこの液に浸漬し、経時的に観察し、次表の結果を得
た。
Freshness Preservation Test The freshness preservation agent prepared above (4000 unit's
/m! 1) was used to investigate the freshness retention effect of burdock root and lettuce. A cross section of a burdock root and a cross section of a lettuce stem base were immersed in this solution and observed over time, and the results shown in the table below were obtained.

【図面の簡単な説明】[Brief explanation of drawings]

図1はポリフェノール酸化酵素阻害斉1]の阻害様式を
示したものである。
Figure 1 shows the inhibition mode of polyphenol oxidase inhibition scheme 1].

Claims (6)

【特許請求の範囲】[Claims] (1)植物の抽出液に含まれるポリフェノール酸化酵素
阻害剤を有効成分とする植物の鮮度保持剤。
(1) A plant freshness-preserving agent whose active ingredient is a polyphenol oxidase inhibitor contained in a plant extract.
(2)植物がアブラナ科に属する植物である、第1請求
項記載の植物の鮮度保持剤。
(2) The plant freshness-preserving agent according to claim 1, wherein the plant belongs to the Brassicaceae family.
(3)ポリフェノール酸化酵素阻害剤が、蛋白を除去し
た植物抽出物をアスコルビン酸酸化酵素で処理して得ら
れたものである第1および第2請求項記載の植物鮮度保
持剤。
(3) The plant freshness-preserving agent according to claims 1 and 2, wherein the polyphenol oxidase inhibitor is obtained by treating a protein-removed plant extract with ascorbic acid oxidase.
(4)ポリフェノール酸化酵素阻害剤が、少なくとも1
00℃、60分間の加熱で阻害活性の低下を示さないも
のである第1ないし第3請求項記載の鮮度保持剤。
(4) at least one polyphenol oxidase inhibitor
The freshness-preserving agent according to any one of claims 1 to 3, which shows no decrease in inhibitory activity when heated at 00°C for 60 minutes.
(5)ポリフェノール酸化酵素阻害剤が、拮抗阻害型で
ある第1ないし第4請求項記載の鮮度保持剤。
(5) The freshness-preserving agent according to any one of claims 1 to 4, wherein the polyphenol oxidase inhibitor is a competitive inhibitory type.
(6)鮮度保持が植物の褐変を抑制するものである、第
1ないし第5請求項記載の鮮度保持剤。
(6) The freshness-preserving agent according to any one of claims 1 to 5, wherein the freshness-preserving agent suppresses browning of plants.
JP1055924A 1989-03-08 1989-03-08 Method for keeping freshness of plant Pending JPH02234638A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1055924A JPH02234638A (en) 1989-03-08 1989-03-08 Method for keeping freshness of plant

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1055924A JPH02234638A (en) 1989-03-08 1989-03-08 Method for keeping freshness of plant

Publications (1)

Publication Number Publication Date
JPH02234638A true JPH02234638A (en) 1990-09-17

Family

ID=13012654

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1055924A Pending JPH02234638A (en) 1989-03-08 1989-03-08 Method for keeping freshness of plant

Country Status (1)

Country Link
JP (1) JPH02234638A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100398628B1 (en) * 2000-08-21 2003-09-19 손석민 Polyphenol oxidase activity inhibition composite containing rhubarb juice and antibrowsing method
US8338339B2 (en) * 2003-12-15 2012-12-25 The United States Of America As Represented By The Secretary Of Agriculture Process for inhibition of proteolytic activity during the ensiling of forages

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5985279A (en) * 1982-11-08 1984-05-17 Seiwa Kasei Kk Preventing animal or plant from blackening or browning
JPS6326942A (en) * 1986-07-07 1988-02-04 ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン Lamp with flexible cover supporter
JPS63160546A (en) * 1986-12-25 1988-07-04 Tottori Pref Gov Method for controlling putrefaction of pickle
JPS63201108A (en) * 1987-02-14 1988-08-19 Ichimaru Pharcos Co Ltd Production of water-soluble extract extracted from flower or seed of safflower

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5985279A (en) * 1982-11-08 1984-05-17 Seiwa Kasei Kk Preventing animal or plant from blackening or browning
JPS6326942A (en) * 1986-07-07 1988-02-04 ジ−・テイ−・イ−・プロダクツ・コ−ポレイシヨン Lamp with flexible cover supporter
JPS63160546A (en) * 1986-12-25 1988-07-04 Tottori Pref Gov Method for controlling putrefaction of pickle
JPS63201108A (en) * 1987-02-14 1988-08-19 Ichimaru Pharcos Co Ltd Production of water-soluble extract extracted from flower or seed of safflower

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100398628B1 (en) * 2000-08-21 2003-09-19 손석민 Polyphenol oxidase activity inhibition composite containing rhubarb juice and antibrowsing method
US8338339B2 (en) * 2003-12-15 2012-12-25 The United States Of America As Represented By The Secretary Of Agriculture Process for inhibition of proteolytic activity during the ensiling of forages

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