CN108795828A - A kind of composite biological agent and preparation method thereof - Google Patents
A kind of composite biological agent and preparation method thereof Download PDFInfo
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- CN108795828A CN108795828A CN201810744265.0A CN201810744265A CN108795828A CN 108795828 A CN108795828 A CN 108795828A CN 201810744265 A CN201810744265 A CN 201810744265A CN 108795828 A CN108795828 A CN 108795828A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The present invention relates to biotechnologies, more particularly to a kind of composite biological agent and preparation method thereof, by anaerobism, constant temperature, cycle, zymotechnique, using aerobic microorganism bacterium and dislike oxygen microbial bacteria in the symbiosis activity decomposition function of happy oxygen technique, generate microbial activity available nutrient, after combining the happy aerobe fermentation of strain, the generation of pathogen can directly be inhibited, decomposable asymmetric choice net organic substance nutrient, easily by crop, plant absorption utilizes, enhance crop, resistance and immunity of the plant to physiological disturbance, improve crop, the photosynthesis of plant leaf surface accumulates.The residual seedling of chemical pesticide can be removed using this composite biological agent, bacillary physiological disturbance inhibits to administer the infringement of crop viral transmission, enhancing crop, landscape ecology period autoimmunity, resistance, disease resistance；Commodity output and quality are improved, disease fruit is reduced and falls off with physiology；To the soil remediation of continuous cropping disease, the active energy of edaphon is replied, reduces the generation of native infectious disease.
Description
Technical field
The present invention relates to biotechnologies, more specifically, are related to a kind of composite biological agent and preparation method thereof.
Background technology
In modern, chemical fertilizer is widely used, and chemical fertilizer is that the volume increase of grain provides a great help, and is free of in chemical fertilizer
There are organic matter, humus, follow and largely use chemical fertilizer, soil is due to the shortage of organic matter and humus, soil aggregate structure incident
To destruction, soil hardening, crop yield declines, and a large amount of of chemical fertilizer use the decline for also resulting in crops quality, endanger simultaneously
Victimize body street health.
Microorganism has gradually been recognized by people in effect agriculturally, and the application of microbial manure is to human body and plant
It is harmless, while soil microenvironment will not be destroyed, peasant's input is reduced, product quality is improved, reduces environmental pollution, have
Economic well, society and ecological benefits.
Invention content
In the presence of overcoming the shortcomings of the prior art, the present invention provide it is a kind of it is nuisanceless, without the residual seedling of chemical contamination, nothing
Big air stream contamination, environment-friendly type composite biological agent and preparation method thereof without side-effects, solve existing biological agent and have a single function,
The problems such as fabrication cycle is long.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of composite biological agent, strain used in said preparation includes swamp Rhodopseudomonas, bacillus megaterium, lactic acid bacteria, vinegar
Acidfast bacilli, saccharomycete and actinomyces.
A kind of preparation method of composite biological agent, includes the following steps：
S1, by swamp Rhodopseudomonas, bacillus megaterium, lactic acid bacteria, acetobacter, saccharomycete and actinomyces through expand breeding
Prepare composite bacteria；
Additional material is added in S2, the composite bacteria for preparing S1 and water carries out constant-temperatureanaerobic anaerobic at a temperature of 20-25 DEG C and ferments；
S3, the composite bacteria of anaerobic fermentation in S2 is filtered, takes filtrate packaging and storage.
Expand in the S1 breeding the specific steps are：It is 5-15 parts of swamp Rhodopseudomonas, huge gemma bar by parts by weight
45-55 parts of bacterium, 10-20 parts of lactic acid bacteria, 10-20 parts of acetobacter, the strain of 10-20 parts of 10-20 parts of saccharomycete and actinomyces are mixed
It closes, fluid nutrient medium is added in oscillation, is placed in vertical constant-temperature table closing culture.
The fluid nutrient medium is composed of the following raw materials by weight：Protein 15 part, 35 parts of glucose, 5000 parts of distilled water and
20 parts of yeast extract.
The fluid nutrient medium pH is 5-6.
The vertical shaking table culture temperature of composite bacteria is 25-30 DEG C, off-period 3-4h.
The additional material includes the bone meal that mass percent is 10%, 5% fish meal, 75% beans amino acid, 5% sugar
Honey, 5% corn flour.
The additional material is also added with synergist and pH adjusting agent.
The anaerobic fermentation time is 7 days in the S2, recycles 4h daily.
Compared with prior art, the advantageous effect of present invention is that：
The present invention provides a kind of composite biological agents and preparation method thereof to use by anaerobism, constant temperature, cycle, zymotechnique
Aerobic microorganism bacterium generates carbohydrate, amino acid with oxygen microbial bacteria is disliked in the symbiosis activity decomposition function of happy oxygen technique
The microbial activities available nutrient such as class, vitamins, nitrogen, albumen, after combining the happy aerobe fermentation of strain, the antibiotic object of generation
Matter can directly inhibit the generation of pathogen, decomposable asymmetric choice net organic substance nutrient easily to be utilized by crop, plant absorption, enhancing crop,
Plant improves the photosynthesis accumulation of crop, plant leaf surface to the resistance and immunity of physiological disturbance.It is carried using the present invention
The composite biological agent of confession can remove the residual seedling of chemical pesticide, and bacillary physiological disturbance inhibits improvement crop viral transmission to invade
Evil, enhancing crop, landscape ecology period autoimmunity, resistance, disease resistance；Commodity output and quality are improved, disease fruit is reduced
It falls off with physiology；To the soil remediation of continuous cropping disease, the active energy of edaphon is replied, reduces the generation of native infectious disease.
Specific implementation mode
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
The every other embodiment that technical staff is obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
A kind of composite biological agent, strain used include swamp Rhodopseudomonas, bacillus megaterium, lactic acid bacteria, acetobacter,
Saccharomycete and actinomyces.
A kind of preparation method of composite biological agent, includes the following steps：
S1, by parts by weight be 15 parts of swamp Rhodopseudomonas, 55 parts of bacillus megaterium, 20 parts of lactic acid bacteria, 20 parts of acetobacter,
20 parts of saccharomycete and the strain of 20 parts of actinomyces mix, and fluid nutrient medium is added in oscillation, and the fluid nutrient medium is by following weight
Part raw material composition：20 parts of protein 15 part, 35 parts of glucose, 5000 parts of distilled water and yeast extract, fluid nutrient medium pH are 6, are placed in
25-30 DEG C of closing culture 3-4h of vertical constant-temperature table；
The bone meal that mass percent is 10%, 5% fish meal, 75% beans amino are added in S2, the composite bacteria for preparing S1
Acid, 5% molasses, the additional material of 5% corn flour add pH and adjust 35 grams with 50 grams of synergist and water progress at 20-25 DEG C
At a temperature of constant-temperatureanaerobic anaerobic ferment 7 days, recycle 4h daily；
S3, the composite bacteria of anaerobic fermentation in S2 is filtered, takes filtrate packaging and storage.
Embodiment 2
A kind of composite biological agent, strain used include swamp Rhodopseudomonas, bacillus megaterium, lactic acid bacteria, acetobacter,
Saccharomycete and actinomyces.
A kind of preparation method of composite biological agent, includes the following steps：
S1, by parts by weight be 5 parts of swamp Rhodopseudomonas, 45 parts of bacillus megaterium, 10 parts of lactic acid bacteria, 10 parts of acetobacter,
10 parts of saccharomycete and the strain of 10 parts of actinomyces mix, and fluid nutrient medium is added in oscillation, and the fluid nutrient medium is by following weight
Part raw material composition：20 parts of protein 15 part, 35 parts of glucose, 5000 parts of distilled water and yeast extract, fluid nutrient medium pH are 5, are placed in
25-30 DEG C of closing culture 3-4h of vertical constant-temperature table；
The bone meal that mass percent is 10%, 5% fish meal, 75% beans amino are added in S2, the composite bacteria for preparing S1
Acid, 5% molasses, the additional material and water of 5% corn flour carry out constant-temperatureanaerobic anaerobic at a temperature of 20-25 DEG C and ferment 7 days, follow daily
Ring 4h；
S3, the composite bacteria of anaerobic fermentation in S2 is filtered, takes filtrate packaging and storage.
Embodiment 3
A kind of composite biological agent, strain used include swamp Rhodopseudomonas, bacillus megaterium, lactic acid bacteria, acetobacter,
Saccharomycete and actinomyces.
A kind of preparation method of composite biological agent, includes the following steps：
S1, by parts by weight be 10 parts of swamp Rhodopseudomonas, 50 parts of bacillus megaterium, 15 parts of lactic acid bacteria, 15 parts of acetobacter,
15 parts of saccharomycete and the strain of 15 parts of actinomyces mix, and fluid nutrient medium is added in oscillation, and the fluid nutrient medium is by following weight
Part raw material composition：20 parts of protein 15 part, 35 parts of glucose, 5000 parts of distilled water and yeast extract, fluid nutrient medium pH are 5-6, are set
In 25-30 DEG C of closing culture 3-4h of vertical constant-temperature table；
The bone meal that mass percent is 10%, 5% fish meal, 75% beans amino are added in S2, the composite bacteria for preparing S1
Acid, 5% molasses, the additional material and water of 5% corn flour carry out constant-temperatureanaerobic anaerobic at a temperature of 20-25 DEG C and ferment 7 days, follow daily
Ring 4h；
S3, the composite bacteria of anaerobic fermentation in S2 is filtered, takes filtrate packaging and storage.
It is used to soak seed using above-mentioned composite biological agent, dress seed, foliage-spray, painting bar, pouring root and punching are applied.
Apple is acted on, fruit tree putrefaction disease regulation effect is up to 90% or more, and commodity rate increases by 20%, and income increases 500-
1000 yuan/mu；Peach is acted on, peach gummosis inhibits regulation effect up to 90%, and commodity rate increases by 25%, and income increases
Mu；Act on grape, grape fruit split inhibits regulation effect up to 90% or more, gray mold, powdery mildew inhibition
Up to 80% or more, commodity rate increases by 30% or more, and sugar content improves 1-3 degree, and income increases by 3000/ mu；Tomato is acted on,
Without special-shaped fruit, hollow fruit, early epidemic, late blight, continuous cropping, root nematode, inhibition are notable, and commodity rate improves 35%, and income increases
It is more than 3000/ mu of member；Cucumber is acted on, mosaic disease, droop inhibition is up to 90%, and commodity rate improves 20%, and income increases
Add 2000 yuan/mu or more.
Only presently preferred embodiments of the present invention is explained in detail above, but the present invention is not limited to above-described embodiment,
Within the knowledge of a person skilled in the art, it can also make without departing from the purpose of the present invention each
Kind variation, various change should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of composite biological agent, it is characterised in that：Strain used in the biological agent includes swamp Rhodopseudomonas, huge bud
Spore bacillus, lactic acid bacteria, acetobacter, saccharomycete and actinomyces.
2. a kind of preparation method of composite biological agent, which is characterized in that include the following steps：
S1, by swamp Rhodopseudomonas, bacillus megaterium, lactic acid bacteria, acetobacter, saccharomycete and actinomyces through expand breeding
Prepare composite bacteria；
Additional material is added in S2, the composite bacteria for preparing S1 and water carries out constant-temperatureanaerobic anaerobic at a temperature of 20-25 DEG C and ferments；
S3, the composite bacteria of anaerobic fermentation in S2 is filtered, takes filtrate packaging and storage.
3. a kind of preparation method of composite biological agent according to claim 2, which is characterized in that expand in the S1 numerous
Grow the specific steps are：It is 5-15 parts of swamp Rhodopseudomonas, 45-55 parts of bacillus megaterium, lactic acid bacteria 10-20 by parts by weight
The strain of part, 10-20 parts of acetobacter, 10-20 parts of saccharomycete and 10-20 parts of actinomyces mixes, and Liquid Culture is added in oscillation
Base is placed in vertical constant-temperature table closing culture.
4. a kind of preparation method of composite biological agent according to claim 3, which is characterized in that the fluid nutrient medium
It is composed of the following raw materials by weight：20 parts of protein 15 part, 35 parts of glucose, 5000 parts of distilled water and yeast extract.
5. a kind of preparation method of composite biological agent according to claim 3, it is characterised in that：The fluid nutrient medium
PH is 5-6.
6. a kind of preparation method of composite biological agent according to claim 3, it is characterised in that：The composite bacteria is vertical
Formula shaking table culture temperature is 25-30 DEG C, and off-period is 3-4 hours.
7. a kind of preparation method of composite biological agent according to claim 2, it is characterised in that：The additional material includes
The bone meal that mass percent is 10%, 5% fish meal, 75% amino acid, 5% molasses, 5% corn flour.
8. a kind of preparation method of composite biological agent according to claim 7, it is characterised in that：The additional material also adds
Added with synergist and pH adjusting agent.
9. a kind of preparation method of composite biological agent according to claim 2, it is characterised in that：Anaerobism is sent out in the S2
The ferment time is 7 days, and the anaerobic fermentation period is to recycle 4h daily.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810744265.0A CN108795828A (en) | 2018-07-09 | 2018-07-09 | A kind of composite biological agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810744265.0A CN108795828A (en) | 2018-07-09 | 2018-07-09 | A kind of composite biological agent and preparation method thereof |
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| Publication Number | Publication Date |
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| CN108795828A true CN108795828A (en) | 2018-11-13 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876806A (en) * | 2005-06-10 | 2006-12-13 | 韩国农村振兴厅 | Bacillus KR083 helpful for crop growth and microbe preparation containing the same |
| CN101445401A (en) * | 2008-04-10 | 2009-06-03 | 徐纯范 | Manufacturing method of composite microorganism fertilizer |
| CN102153382A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Composite microbial foliar fertilizer spraying agent and using method thereof |
| CN102277347A (en) * | 2011-08-03 | 2011-12-14 | 青岛丰泰海洋生物科技有限公司 | Decomposing inoculant of microorganism organic materials |
| US20150342224A1 (en) * | 2008-04-30 | 2015-12-03 | Xyleco, Inc. | Processing biomass |
-
2018
- 2018-07-09 CN CN201810744265.0A patent/CN108795828A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876806A (en) * | 2005-06-10 | 2006-12-13 | 韩国农村振兴厅 | Bacillus KR083 helpful for crop growth and microbe preparation containing the same |
| CN101445401A (en) * | 2008-04-10 | 2009-06-03 | 徐纯范 | Manufacturing method of composite microorganism fertilizer |
| US20150342224A1 (en) * | 2008-04-30 | 2015-12-03 | Xyleco, Inc. | Processing biomass |
| CN102153382A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Composite microbial foliar fertilizer spraying agent and using method thereof |
| CN102277347A (en) * | 2011-08-03 | 2011-12-14 | 青岛丰泰海洋生物科技有限公司 | Decomposing inoculant of microorganism organic materials |
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Application publication date: 20181113 |