CN108770737A - A kind of tiger dragon hybridization spot pond method for culturing seedlings of high-survival rate and growth rate - Google Patents
A kind of tiger dragon hybridization spot pond method for culturing seedlings of high-survival rate and growth rate Download PDFInfo
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- CN108770737A CN108770737A CN201810498747.2A CN201810498747A CN108770737A CN 108770737 A CN108770737 A CN 108770737A CN 201810498747 A CN201810498747 A CN 201810498747A CN 108770737 A CN108770737 A CN 108770737A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention provides the pond method for culturing seedlings of a kind of high-survival rate and the tiger dragon hybridization spot of growth rate, including the selection in nursery pond, regulating and controlling water quality, breeding, illumination adjustment, bait feeding.Present invention employs unique water quality comprehensive regulation technologies, feed by using suitable biological feed and proportioning and accurately sequential, achieve the purpose that the high-survival rate and growth rate of training seedling.Using method for culturing seedlings of the present invention, after nursery 30 days, the survival rate of tiger dragon hybridization spot is up to 99.93%, and coefficient of condition is up to 5.45g/cm3, crude protein content is up to 50.13%, and crude fat content is up to 50.13%, the survival rate 75.82%-81.88% with comparative example, coefficient of condition 2.90-3.52g/cm3, crude protein content 42.49%-45.90%, crude fat content 6.52%-7.63% compare, and survival rate, coefficient of condition, crude protein content and the crude fat content of tiger dragon hybridization spot significantly improve in the present invention.
Description
Technical field
The present invention relates to technical field of aquaculture more particularly to a kind of pond nursery sides of tiger dragon hybridization spot new varieties
Method.
Background technology
It is maternal, epinephelus lanceolatus fish hero body that brave dragon hybridization spot, which is with the female body of epinephelus fuscoguttatus respectively through 2 generation unexpected mass incidents,
For male parent, hybridize the F1 generation of acquisition.Small as maternal epinephelus fuscoguttatus artificial propagation difficulty, nursery survival rate is high, but grows
Speed is slow, and the culture-cycle is long, causes culture efficiency undesirable.Epinephelus lanceolatus fish as male parent be in grouper family individual most
Greatly, the most fast type of the speed of growth, but parent fish rearing and seed breeding difficulty are big, and survival rate of seedling is very low.Dragon and tiger hybridizes spot
Integrate male parent and maternal advantage, main feature：First, the speed of growth is fast；Second is that accommodative ability of environment is strong, survival rate
It is high；Third, meat is good, traction is high, potentiality are big.But for the prior art, different method for culturing seedlings is often deposited
Larger difference on motility rate, the speed of growth, there is presently no a kind of generally acknowledged optimal nursery scheme, nursery result is irregular.
Therefore, a kind of pond method for culturing seedlings that can improve the fast tiger dragon hybridization spot of survival rate, the speed of growth of research is grinding for this field
Study carefully hot spot.
Invention content
For in place of the above the deficiencies in the prior art, the present invention provides a kind of tiger dragon hybridization spot of high-survival rate and growth rate
Pond method for culturing seedlings.
The technical solution adopted by the present invention is as follows：
A kind of tiger dragon hybridization spot pond method for culturing seedlings of high-survival rate and growth rate, includes the following steps：
1) selection in nursery pond；Selection area is 2-3 mus, the pond of depth of water 1-2.5m is as nursery pond.
2) regulating and controlling water quality：Before newly hatched larvae is put into pond, the compound bacteria of 0.01-0.02mg/L, 5-15d are first added into pond
Between add the compound bacteria+0.02-0.05mg/L of 0.01-0.03mg/L every other day and split pot algae solution, 16-24d adds daily
Micro- quasi- ball algae+1-3 μ g/L taurine+1-2 μ g/L citric acids of the compound bacteria+0.01-0.015mg/L of 0.01-0.03mg/L,
25-30d adds the chlorella+1-2 μ g/L salicylic acids of the compound bacteria+0.05-0.1mg/L of 0.01-0.03mg/L daily,
0.1-0.3 μ g/L aluminium chloride ferrums are added in 25d；It is deactivated after 30d；Regulate and control pH value, water Inversion phenomenon and dissolved oxygen amount；Nursery 6-10d is daily
Clean sea water 10% is replaced, nursery 11-15d replaces seawater 20-30% daily, and nursery 16-24d replaces seawater 42-45% daily,
Nursery 25-30d replaces seawater 80-85% daily；
3) breeding；
4) illumination adjusts：The illumination of pond surface requires 5000-8000lx during nursery；
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius, Copepods；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed cladocera and Copepods；It feeds 2-3 times daily；
Preferably, the step 1) is：Choose that area is 2-3 mus, the pond of depth of water 1.5-2.5m is as nursery pond,
Away from one circle gas charging stone of setting at nursery pond edge 1.5-2m, the spacing of adjacent gas charging stone is 2-3m.
Preferably, the method for regulation and control pH value, water Inversion phenomenon and dissolved oxygen amount is in the step 2)：Nursery 0-9 days, pH controls
System is between 7.0-8.5；After nursery 10 days, pH is controlled between 7.0-7.5；Condition of water quality control is in salinity during nursery
17-35,25-33 DEG C of water temperature, dissolved oxygen in water are more than 5ppm.
Preferably, in the step 2), it is that photosynthetic bacteria, saccharomycete and nitrification are thin to regulate and control the compound bacteria being added when water quality
The ratio of bacterium, three kinds of bacterium is 3-2:1:1.
Preferably, the step 3) is：
A, incubator is set：Incubator specification is 4m × 3m × 1m or 6m × 3m × 1m, is arranged in nursery pond, hatching
The upper edge of case is higher by nursery pond 0.1~0.15cm of water level, and in incubator, gas charging stone 1 is uniformly arranged by every square metre of area
Only, the gas charging stone is connected to inflator, and is covered with the sunshade net of 90% shading rate above incubator, is buying fertilized eggs
The same day, seawater is poured into incubator after the filtering of 200 mesh filter bags, until incubator water level depth reaches 0.85~0.9m,
Open the inflator of incubator；
B, the hatching of fertilized eggs：Fertilized eggs are put into incubator 0.5~1h together with packaging bag, until the water temperature in packaging bag
It is consistent with the water temperature of incubator, packaging bag is dismantled, allows fertilized eggs to enter in incubator and hatches, the hatching density of fertilized eggs is 100
×103~120 × 103Grain/m3, fertilized eggs hatch prelarva after 24~26h, and prelarva, which stays in, cultivates 3d in incubator；
C, prelarva is put into nursery pond.
Preferably, in the step 5), the small wheel animalcule of SS types, L-type wheel animalcule and the fairy shrimp nauplius that feed without
Strengthen.
Preferably, in the step 5), SS type wheel animalcule individual lengths are 120-150 microns, width is 60-90 microns；L-type
Wheel animalcule length is 240-250 microns, width is 150-160 microns；The ratio of cladocera and Copepods is 7:3.
Compared with prior art, the beneficial effects of the invention are as follows：
1, the small wheel animalcule of wheel animalcule SS types, L-type wheel animalcule and the fairy shrimp nauplius that the present invention is fed need not move through nutrition
Strengthen, saves time and cost；
2, the present invention combines the changing rule of nursery different times water body and nursery, and different algae is added during nursery and matches
It closes photosynthetic bacteria, saccharomycete and nitrobacteria to use, algae and mushroom synergic adjustment water quality, the production of disease during prevention nursery
It is raw, while algae is wheel animalcule, fairy shrimp nauplius provides nutrition, maintains the continued propagation of wheel animalcule, fairy shrimp nauplius, from
And ensures fry and obtain sufficient bait.
3, nursery 16-24d adds compound bacteria, micro- quasi- ball algae, taurine and citric acid daily, and taurine, which not only has, to be adjusted
Water body pH, the effect for promoting fry and wheel animalcule growth, and being capable of further regulating water quality with citric acid synergistic effect.
4, nursery 25-30d adds compound bacteria, chlorella and salicylic acid daily, and salicylic acid shows fungicidal action, and
It can promote the growth of chlorella, adjust water pH value, the growth conducive to fry.
5, using method for culturing seedlings of the present invention, after nursery 30 days, weight, survival rate, coefficient of condition, the thick egg of tiger dragon hybridization spot
Bai Hanliang and crude fat content significantly improve, and survival rate is up to 99.93%, and coefficient of condition is up to 5.45g/L3, crude protein content
Up to 50.13%, crude fat content is up to 8.96%.
Specific implementation mode
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1：
A kind of tiger dragon hybridization spot pond method for culturing seedlings of high-survival rate and growth rate, includes the following steps：
1) selection in nursery pond:Selection area is 3 mu, the pond of depth of water 2.0m is as nursery pond, away from nursery pond
One circle gas charging stone of setting at the edge 2m of the side of a pond, the spacing of adjacent gas charging stone is 2m.
2) regulating and controlling water quality：Before newly hatched larvae is put into, the compound bacteria (photosynthetic bacteria of 0.02mg/L is first added into pond：Yeast
Bacterium：Nitrobacteria=3:1:1) that, adds the compound bacteria+0.03mg/L of 0.02mg/L between 5-15d every other day splits pot algae,
Micro-+1 μ g/L citric acids of+2 μ g/L taurines of quasi- ball algae of the compound bacteria+0.015mg/L of 0.02mg/L are added after 16-24d daily,
25-30d adds+2 μ g/L salicylic acids of chlorella of the compound bacteria+0.05mg/L of 0.02mg/L daily, and 0.3 μ g/ are added in 25d
L aluminium chloride ferrums；Nursery 0-5 days, pH are controlled between 8.0；After nursery 6 days, pH is controlled between 7.2；Water quality during nursery
Condition controls salinity 30,28 DEG C of water temperature, dissolved oxygen in water and is more than 5ppm；Nursery 6-10d replaces clean sea water 10%, nursery daily
11-15d replaces seawater 20% daily, and nursery 16-24d replaces seawater 45% daily, and nursery 25-30d replaces seawater 80% daily；
3) breeding：
A, incubator is set：Incubator is made of colour bar cloth, rectangle, and specification is 4m × 3m × 1m, is arranged in nursery pond
In the pool, the upper edge of incubator is higher by nursery pond water level 0.15cm, and in incubator, inflation is uniformly arranged by every square metre of area
1, stone, the gas charging stone are connected to inflator, and are covered with the sunshade net of 90% shading rate above incubator, buy by
The same day of smart ovum extracts clean seawater from sea area, and seawater pours into incubator after filter bag filtering made of 200 mesh sieve tulles,
Until incubator water level depth reaches 0.9m, the inflator of incubator is opened, continual inflation is made to incubator water body；
B, the hatching of fertilized eggs：The fertilized eggs bought are put into incubator 0.5h together with packaging bag, until the water in packaging bag
Temperature is consistent with the water temperature of incubator, dismantles packaging bag, allows fertilized eggs to enter in incubator and hatch, the hatching density of fertilized eggs is
100×103Grain/m3, under gassed conditions, fertilized eggs roll in water body, and the timing since fertilization, fertilized eggs are hatched after for 24 hours
Go out prelarva, prelarva stays in after hatching and cultivates 3d in incubator；
C, prelarva is put into nursery pond：After prelarva membrane when 4 age in days, the inflator work for opening nursery pond uninterruptedly fills
Gas unlocks incubator from fixed frame, appoints four edge portions of incubator to sink under the horizontal plane of nursery pond, in incubator
Gas charging stone continue to inflate, appoint a prelarva slowly to be swum under flow action into nursery pond, after 2.5h, wait for that the prelarva in incubator is complete
Portion enters nursery pond, removes incubator；
4) illumination adjusts：Before 10 age in days of prelarva, noon pond face illumination 8000lx；
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and wheel animalcule individual lengths are 120-150 microns, width 60-90
Micron, daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and individual lengths are 240-250 microns, width is 150-160 micro-
Rice, daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius, Copepods；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed cladocera and Copepods (7:3)；It feeds 2 times daily.
Embodiment 2：
A kind of tiger dragon hybridization spot pond method for culturing seedlings of high-survival rate and growth rate, includes the following steps：
1) selection in nursery pond:Selection area is 2 mu, the pond of depth of water 1.5m is as nursery pond, away from nursery pond
One circle gas charging stone of setting at the edge 1.5m of the side of a pond, the spacing of adjacent gas charging stone is 2m.
2) regulating and controlling water quality：Before newly hatched larvae is put into, the compound bacteria (photosynthetic bacteria of 0.01mg/L is first added into pond：Yeast
Bacterium：Nitrobacteria=3:1:1) that, adds the compound bacteria+0.02mg/L of 0.01mg/L between 5-15d every other day splits pot algae,
Micro-+1 μ g/L citric acids of+1 μ g/L taurines of quasi- ball algae of the compound bacteria+0.01mg/L of 0.01mg/L are added after 16-24d daily,
0.1 μ is added in 25d in+1 μ g/L salicylic acids of chlorella for adding the compound bacteria+0.05mg/L of 0.01mg/L after 25-30d daily
G/L aluminium chloride ferrums；It is deactivated after 30d；Nursery 0-9 days, pH are controlled between 7.0；After nursery 10 days, pH is controlled between 7.0；
Condition of water quality control is more than 5ppm in salinity 17,25 DEG C of water temperature, dissolved oxygen in water during nursery；Nursery 6-10d replaces cleaning daily
Seawater 10%, nursery 11-15d replace seawater 20% daily, and nursery 16-24d replaces seawater 42% daily, and nursery 25-30d is daily
Replace seawater 80%；
3) breeding：
A, incubator is set：Incubator is made of colour bar cloth, rectangle, and specification is 6m × 3m × 1m, is arranged in nursery pond
In the pool, the upper edge of incubator is higher by nursery pond water level 0.1cm, and in incubator, inflation is uniformly arranged by every square metre of area
1, stone, the gas charging stone is connected to inflator, and is covered with the sunshade net of 90% shading rate above incubator, avoids sunlight
It is straight to shine, buy fertilized eggs on the day of, extract clean seawater from sea area, seawater is filtered through filter bag made of 200 mesh sieve tulles
After pour into incubator, until incubator water level depth reaches 0.85m, open the inflator of incubator, incubator water body made not
Intermittent inflation；
B, the hatching of fertilized eggs：The fertilized eggs bought are put into incubator 0.5h together with packaging bag, until in packaging bag
Water temperature is consistent with the water temperature of incubator, dismantles packaging bag, allows fertilized eggs to enter in incubator and hatches, and the hatching density of fertilized eggs is
100×103Grain/m3, under gassed conditions, fertilized eggs roll in water body, and the timing since fertilization, fertilized eggs are hatched after for 24 hours
Go out prelarva, prelarva stays in after hatching and cultivates 3d in incubator；
C, prelarva is put into nursery pond：After prelarva membrane when 4 age in days, the inflator work for opening nursery pond uninterruptedly fills
Gas unlocks incubator from fixed frame, appoints four edge portions of incubator to sink under the horizontal plane of nursery pond, in incubator
Gas charging stone continue to inflate, appoint a prelarva slowly to be swum under flow action into nursery pond, after 2h, wait for that the prelarva in incubator is whole
Into nursery pond, incubator is removed；
4) illumination adjusts：The illumination of pond surface requires 8000lx during nursery；
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and wheel animalcule individual lengths are 120-150 microns, width 60-90
Micron, daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and individual lengths are 240-250 microns, width is 150-160 micro-
Rice, daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed cladocera and Copepods (7:3)；It feeds 2 times daily.
The small wheel animalcule of SS types, L-type wheel animalcule and fairy shrimp nauplius when feeding is without reinforcing.
Embodiment 3：
A kind of tiger dragon hybridization spot pond method for culturing seedlings of high-survival rate and growth rate, includes the following steps：
1) selection in nursery pond:Selection area is 2 mu, the pond of depth of water 2.5m is as nursery pond, away from nursery pond
One circle gas charging stone of setting at the edge 2m of the side of a pond, the spacing of adjacent gas charging stone is 3m.
2) regulating and controlling water quality：Before newly hatched larvae is put into, the compound bacteria (photosynthetic bacteria of 0.02mg/L is first added into pond：Yeast
Bacterium：Nitrobacteria=2:1:1) that, adds the compound bacteria+0.05mg/L of 0.03mg/L between 5-15d every other day splits pot algae,
Micro-+2 μ g/L citric acids of+3 μ g/L taurines of quasi- ball algae of the compound bacteria+0.015mg/L of 0.03mg/L are added after 16-24d daily,
0.3 μ is added in 25d in+2 μ g/L salicylic acids of chlorella for adding the compound bacteria+0.1mg/L of 0.03mg/L after 25-30d daily
G/L aluminium chloride ferrums；It is deactivated after 30d；Nursery 0-9 days, pH are controlled between 8.5；After nursery 10 days, pH is controlled between 7.5；
Condition of water quality control is more than 5ppm in salinity 35,33 DEG C of water temperature, dissolved oxygen in water during nursery；Nursery 6-10d replaces cleaning daily
Seawater 10%, nursery 11-15d replace seawater 30% daily, and nursery 16-24d replaces seawater 45% daily, and nursery 25-30d is daily
Replace seawater 85%；
3) breeding：
A, incubator is set：Incubator is made of colour bar cloth, rectangle, and specification is 6m × 3m × 1m, is arranged in nursery pond
In the pool, the upper edge of incubator is higher by nursery pond water level 0.15cm, and in incubator, inflation is uniformly arranged by every square metre of area
1, stone, the gas charging stone is connected to inflator, and is covered with the sunshade net of 90% shading rate above incubator, avoids sunlight
It is straight to shine, buy fertilized eggs on the day of, extract clean seawater from sea area, seawater is filtered through filter bag made of 200 mesh sieve tulles
After pour into incubator, until incubator water level depth reaches 0.9m, the inflator of incubator is opened, between making not to incubator water body
Disconnected inflation；
B, the hatching of fertilized eggs：The fertilized eggs bought are put into incubator 1h together with packaging bag, until the water in packaging bag
Temperature is consistent with the water temperature of incubator, dismantles packaging bag, allows fertilized eggs to enter in incubator and hatch, the hatching density of fertilized eggs is
120×103Grain/m3, under gassed conditions, fertilized eggs roll in water body, and the timing since fertilization, fertilized eggs are hatched after 26h
Go out prelarva, prelarva stays in after hatching and cultivates 3d in incubator；
C, prelarva is put into nursery pond：After prelarva membrane when 4 age in days, the inflator work for opening nursery pond uninterruptedly fills
Gas unlocks incubator from fixed frame, appoints four edge portions of incubator to sink under the horizontal plane of nursery pond, in incubator
Gas charging stone continue to inflate, appoint a prelarva slowly to be swum under flow action into nursery pond, after 3h, wait for that the prelarva in incubator is whole
Into nursery pond, incubator is removed；
4) illumination adjusts：The illumination of pond surface requires 6000lx during nursery；
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and wheel animalcule individual lengths are 120-150 microns, width 60-90
Micron, daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and individual lengths are 240-250 microns, width is 150-160 micro-
Rice, daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius, Copepods；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed cladocera and Copepods (7:3)；It feeds 3 times daily.
The small wheel animalcule of SS types, L-type wheel animalcule and fairy shrimp nauplius when feeding is without reinforcing.
Comparative example 1：
A kind of pond method for culturing seedlings of tiger dragon hybridization spot, includes the following steps：
1) selection in nursery pond:Selection area is 1 mu, the pond of depth of water 1.0m is as nursery pond, away from nursery pond
One circle gas charging stone of setting at the edge 1.0m of the side of a pond, the spacing of adjacent gas charging stone is 1m.
2) regulating and controlling water quality：Before newly hatched larvae is put into, the compound bacteria (photosynthetic bacteria of 0.005mg/L is first added into pond：Yeast
Bacterium：Nitrobacteria=3:0.5:0.4) that, adds the compound bacteria+0.01mg/L of 0.005mg/L between 5-15d every other day splits pot
Algae adds micro-+0.5 μ g/ of+0.5 μ g/L taurines of quasi- ball algae of the compound bacteria+0.005mg/L of 0.005mg/L after 16-24d daily
L citric acids add+0.8 μ g/L salicylic acids of chlorella of the compound bacteria+0.04mg/L of 0.005mg/L after 25-30d, daily
0.08 μ g/L aluminium chloride ferrums are added in 25d；It is deactivated after 30d；Nursery 0-9 days, pH are controlled between 6.5；After nursery 10 days, pH controls
System is between 6.5；Condition of water quality control is more than 5ppm in salinity 15,20 DEG C of water temperature, dissolved oxygen in water during nursery；Nursery 6-10d
Daily to replace clean sea water 5%, nursery 11-15d replaces seawater 12% daily, and nursery 16-24d replaces seawater 40%, educates daily
Seedling 25-30d replaces seawater 70% daily；
3) breeding：
A, incubator is set：Incubator is made of colour bar cloth, rectangle, and specification is 4m × 3m × 1m, is arranged in nursery pond
In the pool, the upper edge of incubator is concordant with nursery pond water level, and in incubator, gas charging stone 1 is uniformly arranged by every square metre of area
Only, the gas charging stone is connected to inflator, and is covered with the sunshade net of 80% shading rate above incubator, avoids sunlight straight
According to, buy fertilized eggs on the day of, extract clean seawater from sea area, seawater is after filter bag filtering made of 100 mesh sieve tulles
Incubator is poured into, until incubator water level depth reaches 0.7m, opens the inflator of incubator, incubator water body is made uninterrupted
Inflation；
B, the hatching of fertilized eggs：The fertilized eggs bought are put into incubator 0.5~1h together with packaging bag, until in packaging bag
Water temperature it is consistent with the water temperature of incubator, dismantle packaging bag, allow fertilized eggs to enter in incubator and hatch, the hatching density of fertilized eggs
It is 90 × 103Grain/m3, under gassed conditions, fertilized eggs roll in water body, and the timing since fertilization, fertilized eggs are incubated after for 24 hours
Prelarva is dissolved, prelarva stays in after hatching and cultivates 3d in incubator；
C, prelarva is put into nursery pond：After prelarva membrane when 4 age in days, the inflator work for opening nursery pond uninterruptedly fills
Gas unlocks incubator from fixed frame, appoints four edge portions of incubator to sink under the horizontal plane of nursery pond, in incubator
Gas charging stone continue to inflate, appoint a prelarva slowly to be swum under flow action into nursery pond, after 1h, wait for that the prelarva in incubator is whole
Into nursery pond, incubator is removed；
4) illumination adjusts：The illumination of pond surface requires 4000lx during nursery；
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and wheel animalcule individual lengths are 90-110 microns, width is 60-90 micro-
Rice, daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and individual lengths are 200-220 microns, width is 150-160 micro-
Rice, daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius, Copepods；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed cladocera and Copepods (2:8)；It feeds 1 time daily.
The small wheel animalcule of SS types, L-type wheel animalcule and fairy shrimp nauplius when feeding is without reinforcing.
Comparative example 2：
A kind of pond method for culturing seedlings of tiger dragon hybridization spot, includes the following steps：
1) selection in nursery pond:Selection area is 5 mu, the pond of depth of water 3m is as nursery pond, away from nursery pond
One circle gas charging stone of setting at edge 3m, the spacing of adjacent gas charging stone is 4m.
2) regulating and controlling water quality：Before newly hatched larvae is put into, the compound bacteria (photosynthetic bacteria of 0.03mg/L is first added into pond：Yeast
Bacterium：Nitrobacteria=3:5:6) that, adds the compound bacteria+0.07mg/L of 0.05mg/L between 5-15d every other day splits pot algae,
Micro-+4 μ g/L citric acids of+5 μ g/L taurines of quasi- ball algae of the compound bacteria+0.02mg/L of 0.05mg/L are added after 16-24d daily,
0.5 μ is added in 25d in+3 μ g/L salicylic acids of chlorella for adding the compound bacteria+0.2mg/L of 0.05mg/L after 25-30d daily
G/L aluminium chloride ferrums；It is deactivated after 30d；Nursery 0-9 days, pH are controlled between 8.5；After nursery 10 days, pH is controlled between 8.5；
Condition of water quality control is more than 5ppm in salinity 38,25~37 DEG C of water temperature, dissolved oxygen in water during nursery；Nursery 6-10d is replaced daily
Clean sea water 20%, nursery 11-15d replace seawater 35% daily, and nursery 16-24d replaces seawater 50%, nursery 25-30d daily
It is daily to replace seawater 100%；
3) breeding：
A, incubator is set：Incubator is made of colour bar cloth, rectangle, and specification is 4m × 3m × 1m, is arranged in nursery pond
In the pool, the upper edge of incubator is higher by nursery pond water level 0.5cm, and in incubator, inflation is uniformly arranged by every square metre of area
1, stone, the gas charging stone is connected to inflator, and is covered with the sunshade net of 95% shading rate above incubator, avoids sunlight
It is straight to shine, buy fertilized eggs on the day of, extract clean seawater from sea area, seawater is filtered through filter bag made of 300 mesh sieve tulles
After pour into incubator, until incubator water level depth reaches 1m, open the inflator of incubator, incubator water body made uninterrupted
Inflation；
B, the hatching of fertilized eggs：The fertilized eggs bought are put into incubator 2h together with packaging bag, until the water in packaging bag
Temperature is consistent with the water temperature of incubator, dismantles packaging bag, allows fertilized eggs to enter in incubator and hatch, the hatching density of fertilized eggs is
150×103Grain/m3, under gassed conditions, fertilized eggs roll in water body, and the timing since fertilization, fertilized eggs are hatched after for 24 hours
Go out prelarva, prelarva stays in after hatching and cultivates 3d in incubator；
C, prelarva is put into nursery pond：After prelarva membrane when 4 age in days, the inflator work for opening nursery pond uninterruptedly fills
Gas unlocks incubator from fixed frame, appoints four edge portions of incubator to sink under the horizontal plane of nursery pond, in incubator
Gas charging stone continue to inflate, appoint a prelarva slowly to be swum under flow action into nursery pond, after 5h, wait for that the prelarva in incubator is whole
Into nursery pond, incubator is removed；
4) illumination adjusts：The illumination of pond surface requires 10000lx during nursery；
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and wheel animalcule individual lengths are 170-180 microns, width 95-100
Micron, daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and individual lengths are 270-290 microns, width is 180-200 micro-
Rice, daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius, Copepods；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed cladocera and Copepods (7:3)；It feeds 5 times daily.
The small wheel animalcule of SS types, L-type wheel animalcule and fairy shrimp nauplius when feeding is without reinforcing.
Comparative example 3：
A kind of pond method for culturing seedlings of tiger dragon hybridization spot, includes the following steps：
1) selection in nursery pond:It is same as Example 1.
2) regulating and controlling water quality：Before newly hatched larvae is put into, the compound bacteria (photosynthetic bacteria of 0.02mg/L is first added into pond：Nitrification
Bacterium=3:1:2) that, adds the compound bacteria+0.03mg/L of 0.02mg/L between 5-15d every other day splits pot algae, after 16-24d
Micro- quasi- ball algae of the compound bacteria+0.015mg/L of 0.02mg/L is added daily, adds the compound bacteria of 0.02mg/L after 25-30d daily
The chlorella of+0.05mg/L；It is deactivated after 30d；Nursery 0-9 days, pH are controlled between 8.0；After nursery 10 days, pH controls exist
Between 7.2；Condition of water quality control is more than 5ppm in salinity 30,28 DEG C of water temperature, dissolved oxygen in water during nursery；Nursery 6-10d is daily
Clean sea water 10% is replaced, nursery 11-15d replaces seawater 20% daily, and nursery 16-24d replaces seawater 45%, nursery daily
25-30d replaces seawater 80% daily；
Step 3), step 4) are same as Example 1.
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and wheel animalcule individual lengths are 120-150 microns, width 60-90
Micron, daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and individual lengths are 240-250 microns, width is 150-160 micro-
Rice, daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed Copepods；It feeds 2 times daily.
The small wheel animalcule of SS types, L-type wheel animalcule and fairy shrimp nauplius when feeding is without reinforcing.
Experimental example：
The method that embodiment and comparative example is respectively adopted carries out nursery, after nursery 30 days, measure each group prelarva body at
Point, survival rate and coefficient of condition.
Survival rate=juvenile fish number at the end of experiment/puts seedling prelarva number × 100%；
Coefficient of condition K=(m/L3)×100；
M is weight (g) in formula, and L is that body grows (cm)；
Measurement result is shown in Tables 1 and 2.
Table 1
Table 2
| Moisture (%) | Crude protein (%) | Crude fat (%) | |
| Embodiment 1 | 80.93±0.24 | 50.13±0.93 | 8.96±0.33 |
| Embodiment 2 | 80.45±0.33 | 49.01±0.84 | 8.72±0.53 |
| Embodiment 3 | 81.30±0.34 | 50.08±0.85 | 8.50±0.64 |
| Comparative example 1 | 80.12±0.25 | 45.50±0.64 | 6.52±0.36 |
| Comparative example 2 | 81.05±0.36 | 43.63±0.73 | 7.63±0.54 |
| Comparative example 3 | 81.00±0.43 | 42.49±0.85 | 6.56±0.65 |
From table 1, table 2 result it is found that after nursery 30 days, the weight of present invention tiger dragon hybridization spot, survival rate, grossness
Degree, crude protein content and crude fat content are obviously higher than comparative example, and the nursery effect of wherein 1 group of embodiment is best, survival rate
Up to 99.93%, coefficient of condition is up to 5.45g/cm3, crude protein content is up to 50.13%, crude fat content and is up to 8.96%.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent substitution, improvement and etc. done should be included within the scope of protection of the invention god.
Claims (7)
1. the tiger dragon hybridization spot pond method for culturing seedlings of a kind of high-survival rate and growth rate, which is characterized in that include the following steps：
1) selection in nursery pond；
2) regulating and controlling water quality：Before newly hatched larvae is put into pond, the compound bacteria of 0.01-0.02mg/L is first added into pond, between 5-15d
That adds the compound bacteria+0.02-0.05mg/L of 0.01-0.03mg/L every other day splits pot algae, and 16-24d adds 0.01- daily
Micro- quasi- ball algae+1-3 μ g/L taurine+1-2 μ g/L citric acids of the compound bacteria+0.01-0.015mg/L of 0.03mg/L, 25-30d
Chlorella+1-2 μ g/L the salicylic acids of the compound bacteria+0.05-0.1mg/L of daily addition 0.01-0.03mg/L, are added in 24d
0.1-0.3 μ g/L aluminium chloride ferrums；It is deactivated after 30d；Regulate and control pH value, water Inversion phenomenon and dissolved oxygen amount；Nursery 6-10d replaces clear daily
Clean seawater 10%, nursery 11-15d replace seawater 20-30% daily, and nursery 16-24d replaces seawater 42-45%, nursery daily
25-30d replaces seawater 80-85% daily；
3) breeding；
4) illumination adjusts：The illumination of pond surface requires 5000-8000lx during nursery；
5) it feeds：
A, 3 age in days of prelarva to 5 ages in days feed SS type wheel animalcules, and daily 8:00-8:It is fed between 30 primary；
B, 6 age in days of prelarva to 15 ages in days feed L-type wheel animalcule, and daily 8:00-8:It is fed between 30 primary；
C, 16 ages in days to 24 ages in days feed fairy shrimp nauplius, Copepods；Daily 8:00-8:It is fed between 30 primary；
D, 25 ages in days to 30 ages in days feed cladocera and Copepods；It feeds 2-3 times daily.
2. the tiger dragon hybridization spot pond method for culturing seedlings of a kind of high-survival rate according to claim 1 and growth rate, feature
It is, the step 1) is：Selection area is 2-3 mus, the pond of depth of water 1.5-2.5m is as nursery pond, away from nursery pond
One circle gas charging stone of setting at edge 1.5-2m, the spacing of adjacent gas charging stone is 2-3m.
3. the tiger dragon hybridization spot pond method for culturing seedlings of a kind of high-survival rate according to claim 1 and growth rate, feature
It is, the method for regulation and control pH value, water Inversion phenomenon and dissolved oxygen amount is in the step 2)：Nursery 0-9 days, pH are controlled in 7.0-8.5
Between；After nursery 10 days, pH is controlled between 7.0-7.5；Condition of water quality control is in salinity 17-35, water temperature 25- during nursery
33 DEG C, dissolved oxygen in water be more than 5ppm.
4. the tiger dragon hybridization spot pond method for culturing seedlings of a kind of high-survival rate according to claim 1 and growth rate, feature
It is, it is photosynthetic bacteria, saccharomycete and nitrobacteria to regulate and control the compound bacteria being added when water quality, and the ratio of three kinds of bacterium is 3-2:1:1.
5. the tiger dragon hybridization spot pond method for culturing seedlings of a kind of high-survival rate according to claim 1 and growth rate, feature
It is, the step 3) is：
A, incubator is set：Incubator specification is 4m × 3m × 1m or 6m × 3m × 1m, is arranged in nursery pond, incubator
Upper edge is higher by nursery pond 0.1~0.15cm of water level, and in incubator, gas charging stone 1, institute are uniformly arranged by every square metre of area
It states gas charging stone and is connected to inflator, and covered with the sunshade net of 90% shading rate above incubator, buying working as fertilized eggs
It, pours into incubator after the filtering of 200 mesh filter bags by seawater, until incubator water level depth reaches 0.85~0.9m, opens
The inflator of incubator；
B, the hatching of fertilized eggs：Put fertilized eggs into incubator 0.5~1h together with packaging bag, until packaging bag in water temperature with incubate
The water temperature for changing case is consistent, dismantles packaging bag, allows fertilized eggs to enter in incubator and hatch, and the hatching density of fertilized eggs is 100 × 103
~120 × 103Grain/m3, fertilized eggs hatch prelarva after 24~26h, and prelarva, which stays in, cultivates 3d in incubator；
C, prelarva is put into nursery pond.
6. the tiger dragon hybridization spot pond method for culturing seedlings of a kind of high-survival rate according to claim 1 and growth rate, feature
It is, the small wheel animalcule of SS types, L-type wheel animalcule and the fairy shrimp nauplius when feeding are without reinforcing.
7. the tiger dragon hybridization spot pond method for culturing seedlings of a kind of high-survival rate according to claim 1 and growth rate, feature
It is, in the step 5), SS type wheel animalcule individual lengths are 120-150 microns, width is 60-90 microns；L-type wheel animalcule length is
240-250 microns, width be 150-160 microns；The ratio of cladocera and Copepods is 7:3.
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Denomination of invention: A seedling raising method of tiger dragon hybrid spot pond with high survival rate and growth rate Effective date of registration: 20210928 Granted publication date: 20201027 Pledgee: CITIC Bank Haikou branch Pledgor: HAINAN CHENHAI AQUATIC Co.,Ltd. Registration number: Y2021980010243 |