CN108684590A - A kind of postoperative low-gravity seawater recuperation method of pteria martensii plant core shellfish - Google Patents
A kind of postoperative low-gravity seawater recuperation method of pteria martensii plant core shellfish Download PDFInfo
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- CN108684590A CN108684590A CN201810784993.4A CN201810784993A CN108684590A CN 108684590 A CN108684590 A CN 108684590A CN 201810784993 A CN201810784993 A CN 201810784993A CN 108684590 A CN108684590 A CN 108684590A
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- 239000013535 sea water Substances 0.000 title claims abstract description 93
- 241000879903 Pteria Species 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000002980 postoperative effect Effects 0.000 title claims abstract description 22
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- 239000000460 chlorine Substances 0.000 claims description 14
- 229910052801 chlorine Inorganic materials 0.000 claims description 14
- 230000003115 biocidal effect Effects 0.000 claims description 12
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 11
- 241000195493 Cryptophyta Species 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- KJHOZAZQWVKILO-UHFFFAOYSA-N N-(diaminomethylidene)-4-morpholinecarboximidamide Chemical compound NC(N)=NC(=N)N1CCOCC1 KJHOZAZQWVKILO-UHFFFAOYSA-N 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 9
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- 239000004576 sand Substances 0.000 claims description 8
- 235000020681 well water Nutrition 0.000 claims description 8
- 239000002349 well water Substances 0.000 claims description 8
- RDEIXVOBVLKYNT-HDZPSJEVSA-N (2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-[(1r)-1-aminoethyl]oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;(2r,3r,4r,5r)-2-[(1s,2s,3r,4s,6r)-4,6-diamino-3-[(2r,3r,6s)-3-amino-6-(aminomethyl)oxan-2 Chemical compound OS(O)(=O)=O.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@@H](CN)O2)N)[C@@H](N)C[C@H]1N.O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H](CC[C@H](O2)[C@@H](C)N)N)[C@@H](N)C[C@H]1N.O1[C@H]([C@@H](C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N RDEIXVOBVLKYNT-HDZPSJEVSA-N 0.000 claims description 7
- QYIYFLOTGYLRGG-GPCCPHFNSA-N cefaclor Chemical compound C1([C@H](C(=O)N[C@@H]2C(N3C(=C(Cl)CS[C@@H]32)C(O)=O)=O)N)=CC=CC=C1 QYIYFLOTGYLRGG-GPCCPHFNSA-N 0.000 claims description 7
- 229960005361 cefaclor Drugs 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 238000007796 conventional method Methods 0.000 claims description 6
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- 239000008399 tap water Substances 0.000 claims description 4
- 235000020679 tap water Nutrition 0.000 claims description 4
- 229930182555 Penicillin Natural products 0.000 claims description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 3
- 239000000645 desinfectant Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229940049954 penicillin Drugs 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 239000012286 potassium permanganate Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 4
- 239000007789 gas Substances 0.000 claims 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000011049 pearl Substances 0.000 abstract description 42
- 239000010984 cultured pearl Substances 0.000 abstract description 11
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 241001212699 Pinctada martensii Species 0.000 abstract description 2
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 230000036541 health Effects 0.000 description 7
- YRIZYWQGELRKNT-UHFFFAOYSA-N 1,3,5-trichloro-1,3,5-triazinane-2,4,6-trione Chemical compound ClN1C(=O)N(Cl)C(=O)N(Cl)C1=O YRIZYWQGELRKNT-UHFFFAOYSA-N 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 229950009390 symclosene Drugs 0.000 description 5
- 241000238557 Decapoda Species 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 241000490567 Pinctada Species 0.000 description 2
- 241000490568 Pinctada fucata Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000009288 screen filtration Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 235000013411 Alpinia speciosa Nutrition 0.000 description 1
- 244000060696 Alpinia speciosa Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
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- 230000037406 food intake Effects 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000009343 monoculture Methods 0.000 description 1
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- 230000028327 secretion Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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- 229960005322 streptomycin Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
- A01K61/56—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
- A01K61/57—Pearl seeds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of pteria martensiis to plant the postoperative low-gravity seawater recuperation method of core shellfish, belongs to technical field of aquaculture.This method basic step includes that the postoperative recuperation pond of pteria martensii prepares, recuperation water is prepared, pearly shellfish selects and plant core, pearly shellfish recuperation management, during pteria martensii plant core is grown cultured pearls, the healthy pteria martensii of selection carries out plant core and grows cultured pearls, indoor or outdoor sink is moved on to after operation and carries out low-gravity seawater recuperation, and sea area is conventionally moved on to after recuperation and carries out pearly shellfish cultivation;Pteria martensii is carried out using this method to recuperate after an operation, the core-remaining rate of operation shellfish is high, high quality pearl rate is high, the pearl quality and economic benefit of pearly shellfish can be improved, the present invention is suitable for the postoperative recuperation of Pinctada martensii pearl breeding shellfish, it is easy to promote and apply in manufacturing enterprise, economic benefit and social benefit are notable.
Description
Technical field
The present invention relates to a kind of pteria martensiis to plant the postoperative low-gravity seawater recuperation method of core shellfish, belongs to aquaculture technology
Field plants core suitable for pteria martensii and grows cultured pearls production.
Background technology
Pteria martensii(Pinctada martensii)Also known as pinctada fucata(Pinctada fucata), it is China
The pearl of the main shellfish kind of breeding seawater pearl, production accounts for 95% or more of sea water pearls yield, is cultivated with pteria martensii
Pearl claims " Nan Zhu ", and the culture zone " Nan Zhu " is located at Guangdong, Guangxi and Hainan, and Guangdong is main producing region.Currently, China " Nan Zhu " is produced
Existing main problem be grow cultured pearls variety deterioration, breeding environment deteriorates and cultural technique is outmoded, lead to that the pearly shellfish death rate is high, stays
Core rate is low, pearl quality is poor, has seriously affected the sustainable development of " Nan Zhu " industry." Nan Zhu " cultivation is Lezhou Peninsula littoral
The valuable feature industry in area, cultured area surpasses 5.38 ten thousand mu when producing peak, and 20 tons or so of pearl annual output accounts for national yield
70% or more, about 4.5 ten thousand people are engaged in pearl sector, have driven pearl shell seedling fostering, female shellfish cultivation, plant core is grown cultured pearls, pearl is processed
With sale etc. industries development, for Lezhou Peninsula coastal area peasant shake off poverty and set out on the road to prosperity and foreign exchange earning is made that positive contribution.
Currently, " Nan Zhu " annual output only has 2-3 tons, the quality and yield of pearl culture significantly glide, and raiser is profitless, pearl
Industry gets into a difficult position.
Pteria martensii operation shellfish, by major injury, if dealt with improperly after operation, can not only lead to hand when planting core
Art shellfish mortality can also influence the pearl quality and yield that survive shellfish.Therefore, the postoperative recuperation after pearl shell plant core is to improve
The important technique measure of pearly shellfish survival rate and core-remaining rate, the purpose of recuperation are that operation shellfish is allowed slowly to get well, and reduce death,
Pearl core is set not occur or occur less position movement, anti-anti-avulsion core and plain pearl, deformity before transplanting small pieces form pearl sac
The generation of pearl.The factors influence such as flow velocity, wave, the variation of water temperature and the type and quantity of food organisms in recuperation place is grown cultured pearls
Shellfish stays core bead yield rate and pearl quality, and the economic benefit of pearl culture mainly by the survival rate of pearly shellfish, stay core bead yield rate
It is determined with high-quality pearl rate, wherein the proportion shared by high-quality pearl rate is maximum.
Female shellfish of performing the operation in production process currently, pteria martensii plant core is grown cultured pearls moves directly to sea area recuperation, due to sea area
The environmental factors such as water temperature, flow and the type and quantity of food organisms have unstability, cause pearly shellfish survival rate low, take off
Core rate is high, and the transplanted cells small pieces formation pearl sac time is long, and nacre secretion speed is slow, and high-quality pearl rate is low, most of raiser
It often plants 10,000 shellfishes of core and harvests 1-2 jins of pearl, high-quality pearl rate only has 15% or so, and raiser is profitless or even runs at a loss, leads to
Cross technological innovation improve pearl cultivating high-quality pearl rate seem very necessary with it is urgent.
Invention content
The object of the present invention is to provide a kind of pteria martensiis to plant the postoperative low-gravity seawater recuperation method of core shellfish, can improve
Pteria martensii plants core shellfish survival rate and high-quality pearl rate, increases economic benefit and social benefit is notable.
Technical scheme is as follows:A kind of postoperative low-gravity seawater recuperation method of pteria martensii plant core shellfish, substantially
Steps are as follows:
(1)The preparation in recuperation pond:Selection has into pumping equipment, high 1.2-1.5m, floor space 20-40m2Indoor pond or
Person's floor space is 30-50 m2Outdoor sink, pond shape are rectangular or round, and outdoor sink needs shelving to install canvas
Or colour bar cloth is taken shelter from rain with shading, volume big water storage in pond is more, and environment is stablized relatively, but long into water discharge time, when cleaning and sterilizing
Between it is long, select suitable pond according into drainage speed, pond is per 3-4m2If gas exhausting stone one, water body is in boiling-like, pond when inflation
The breeding facilities such as son, the gentle stone of tracheae, which are rinsed well before using with fresh water, to be dried 2-3 days, then the processing that carries out disinfection.Commonly
Disinfectant is the chlorine-containing disinfectant or 30-50mg/L liquor potassic permanganates of available chlorine content 25-30mg/L;
(2)The preparation of low-gravity seawater:The natural sea-water that filling is filtered through husky well and sand filter in clean pond, adds well water
Either tap water be configured to proportion be 1.016-1.018 low-gravity seawater acid-base value it is excessively high or too low when sodium bicarbonate
Or salt acid for adjusting pH value 7.8-8.3;
(3)Recuperation is configured with water:By step(2)In the recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well, water
It is deep to keep 1.2-1.3m, it is in boiling-like when water body is inflated;
(4)Pearly shellfish selects and plants core:Operate female shellfish, 1.0-1.5 age shellfishes of selection 1.5-2 age health pteria martensiis makees small pieces
Shellfish carries out plant core according to a conventional method.According to shellfish body size, it is closely packed in upwards not by the density per cage 35-38 only, right shell after operation
It supports in cage, moves on to step(3)Hanging culture in the recuperation pond of low-gravity seawater is injected, for hanging culture depth from bottom of pond 20-30cm, hanging culture is close
Degree is every cube of 3 cage of water body(105-114/m3);
(5)The recuperation management of pearly shellfish:Shellfish of performing the operation checked once per 2-3 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days
Material, does not change water, and the antibiotic and 0.5 mg/L moroxydines of 0.5mg/L-1.0mg/L is used alternatingly, and common antibiotic is sulfuric acid
Gentamicin, Cefaclor, penicillin and streptomysin mixture;Unicellular alga, such as P latymonas subcordifomis are fed on a small quantity after 6th day
10000-20000 cells/mL.d either chlorella 30000-50000 cells/mL.d or filtered through 300 mesh screens
The natural algae 10000-20000 cells/mL.d cultivated in natural sea-water started to change water, day quantity of exchanged water 1/5-1/ after the 6th day
3;With the recovery of shellfish body after 10th day, daily ration, feeding quantity is gradually increased, such as P latymonas subcordifomis 20000-40000 cells/mL.d, or
Chlorella 50000-80000 cells/mL.d, or the mixed algae cultivated in through the filtered natural sea-water of 300 mesh screens
20000-40000 cells/mL.d;The pearly shellfish recuperation time is 22-25 days, and pearly shellfish moves on to sea area and cultivates first 3 days by cultivation use
Low-gravity seawater is changed to natural sea-water, and sea area is conventionally moved on to after recuperation and carries out pearly shellfish cultivation.
The beneficial effects of the invention are as follows:
First, planting core operation stepmother shellfish by serious wound, antibiotic and moroxydine are launched in pond, can prevent from planting core
Wound infection improves survival rate, promotes the quick formation of pearl sac, and antibiotic is used alternatingly, and bacterium is inhibited to generate drug resistance.
Second is that after mother Bei Shoushu, the physically weak weak ingestion ability of shellfish declines, and water is not changed in preceding not bait throwing in 6 days, is the recovery of pearly shellfish
There is provided and stablize clean environment condition, with the extension of recuperation time, gradually increase daily ration, feeding quantity be conducive to shellfish body slowly restore and
It is not to bounce back in a short time, is conducive to improve pearly shellfish core-remaining rate.Before recuperation period terminates, replaces and cultivate low ratio
Weight seawater adapts to sea environment condition by pearly shellfish in advance, then moves on to sea area and grow cultured pearls so that pearly shellfish adapts to sea within a short period of time
Area's environment.
Third, low-gravity seawater is recuperated, white series pearl and high quality pearl rate are accomplished than tradition in the pearl that pearly shellfish is formed
Method improves 10% or more, and pearl surface is smooth, spot is few, and high-quality pearl rate is high.
Fourth, either the idle indoor pond of prawn seed-rearing or outdoor sink carry out planting core mother the usable shellfish of the present invention
Shellfish recuperates after an operation, and required equipment is few, and using common drug, bait can use P latymonas subcordifomis, the chlorella of monoculture,
It can also be used directly in natural through being cultivated in the filtered natural sea-water of 300 mesh screens even the shrimp pool water in prawn culturing pond
Mixed algae, it is easy to operate, it is suitable for pteria martensii artificial pearl culture and produces, economical, societal benefits is notable.
Specific implementation mode
The present invention is described in further details below by embodiment, these embodiments are only used for illustrating the present invention, and
It does not limit the scope of the invention.
Embodiment 1
The method for carrying out the postoperative low-gravity seawater recuperation of pteria martensii using indoor pond
(1)The preparation in recuperation pond:Select floor space 20m2, the rectangular indoor pond of high 1.5m, per 4m2If gas exhausting stone one,
Gas exhausting stone 5 is installed altogether.It is rinsed well and is dried 2 days with fresh water, the installations and facilities such as pond, the gentle stone of tracheae are with available chlorine content
The strong chlorine oil of 25mg/L(Sym-closene)It carries out full pond to disinfect, then clean with filtering seawater flushing.
(2)The preparation of low-gravity seawater:The natural sea-water that filling is filtered through husky well and sand filter in clean pond, addition
Well water is configured to the low-gravity seawater of proportion 1.016, pH value 7.8.
(3)Recuperation is configured with water:By step(2)The recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well
In, the depth of water keeps 1.3m, is in boiling-like when water body is inflated;
(4)Plant core operation:Select operate female shellfish, 1.5 age shellfishes of 2 age health pteria martensiis to make piece shell, according to a conventional method into
Row plants core, and operation shellfish presses the density per 35, cage, and right shell is closely packed in upwards in recuperation cage, moves on to step(3)Inject low-gravity sea
Hanging culture in the recuperation pond of water, for hanging culture depth from bottom of pond 20cm, hanging culture density is every cube of 3 cage of water body(105/m3).
(5)The management of pearly shellfish:Shellfish of performing the operation checks once for every 2 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days,
Water is not changed, the gentamicin sulphate and Cefaclor of 0.5mg/L is used alternatingly(A kind of antibiotic be used continuously change again within 3 days it is another
Kind)And the moroxydine of 0.5mg/L.P latymonas subcordifomis 10000/mL.d is fed after 6th day, is started to change water after the 6th day, be changed day
Water 1/5 feeds 20000 cells of P latymonas subcordifomis/mL.d after the 10th day.The pearly shellfish recuperation time is 22 days, and pearly shellfish moves on to
Sea area cultivates first 3 days cultivation being changed to natural sea-water with low-gravity seawater, other management measures do not change, then according to routine
Method moves on to sea area and carries out pearly shellfish cultivation.
Embodiment 2
The method for carrying out the postoperative low-gravity seawater recuperation of pteria martensii using indoor pond
(1)The preparation in recuperation pond:Select floor space 30m2, pond in the circular cell of high 1.35m, per 3m2If gas exhausting stone one,
Gas exhausting stone 10 is installed altogether.It is rinsed well and is dried 3 days with fresh water, the installations and facilities available chlorine content such as pond, the gentle stone of tracheae
For the strong chlorine oil of 27.5mg/L(Sym-closene)It carries out full pond to disinfect, then clean with filtering seawater flushing.
(2)The preparation of low-gravity seawater:The natural sea-water that filling is filtered through husky well and sand filter in clean pond, addition
Well water is configured to the low-gravity seawater of proportion 1.017, pH value 8.05.
(3)Recuperation is configured with water:By step(2)The recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well
In, the depth of water keeps 1.25m, is in boiling-like when water body is inflated;
(4)Plant core operation:Operate female shellfish, 1.25 age shellfishes of 1.75 age health pteria martensiis are selected to make piece shell, routinely side
Method carries out plant core, and operation shellfish presses the density per 37, cage, and right shell is closely packed in upwards in recuperation cage, moves on to step(3)Inject low ratio
Hanging culture in the recuperation pond of weight seawater, for hanging culture depth from bottom of pond 25cm, hanging culture density is every cube of 3 cage of water body(110/m3).
(5)The management of pearly shellfish:Shellfish of performing the operation checks once for every 2.5 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days
Material, does not change water, the gentamicin sulphate and Cefaclor of 0.75mg/L is used alternatingly(A kind of antibiotic is used continuously 3 days and changes again
It is another)And the moroxydine of 0.5mg/L, it feeds after the 6th day and is cultivated in through the filtered natural sea-water of 300 mesh screens on a small quantity
15000 cells of natural algae/mL.d, start to change water after the 6th day, day quantity of exchanged water fed through 300 mesh after the 1/5, the 10th day
30000 cells of the natural algae/mL.d cultivated in natural sea-water after screen filtration, the pearly shellfish recuperation time is 23 days, is educated
Pearl shellfish moves on to sea area cultivation and cultivation was changed to natural sea-water with low-gravity seawater in first 3 days, other management measures do not change, then
It conventionally moves on to sea area and carries out pearly shellfish cultivation.
Embodiment 3
The method for carrying out the postoperative low-gravity seawater recuperation of pteria martensii using indoor pond
(1)The preparation in recuperation pond:Select floor space 40m2, the rectangular indoor pond of high 1.3m, per 4m2If gas exhausting stone one,
Gas exhausting stone 10 is installed altogether.It is rinsed well and is dried 3 days with fresh water, the installations and facilities available chlorine content such as pond, the gentle stone of tracheae
For the strong chlorine oil of 25mg/L(Sym-closene)It carries out full pond to disinfect, then clean with filtering seawater flushing.
(2)The preparation of low-gravity seawater:The natural sea-water that filling is filtered through husky well and sand filter in clean pond, addition
Well water is configured to the low-gravity seawater of proportion 1.018, pH value 8.3.
(3)Recuperation is configured with water:By step(2)The recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well
In, the depth of water keeps 1.2m, is in boiling-like when water body is inflated;
(4)Plant core operation:Select operate female shellfish, 1.5 age shellfishes of 2 age health pteria martensiis to make piece shell, according to a conventional method into
Row plants core, and operation shellfish presses the density per 35, cage, and right shell is closely packed in upwards in recuperation cage, moves on to step(3)Inject low-gravity sea
Hanging culture in the recuperation pond of water, for hanging culture depth from bottom of pond 20cm, hanging culture density is every cube of 3 cage of water body(105/m3).
(5)The management of pearly shellfish:Shellfish of performing the operation checks once for every 2 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days,
Water is not changed, the gentamicin sulphate and Cefaclor of 1.0mg/L is used alternatingly(A kind of antibiotic be used continuously change again within 3 days it is another
Kind)And the moroxydine of 0.5mg/L, 50000 cells of chlorella/mL.d is fed after the 6th day, is started to change water after the 6th day, be changed day
Water 1/5, chlorella feeding volume is 50000 cells/mL.d after the 10th day, and the pearly shellfish recuperation time is 25 days, and pearly shellfish moves on to
Sea area cultivates first 3 days cultivation being changed to natural sea-water with low-gravity seawater, other management measures do not change, then according to routine
Method moves on to sea area and carries out pearly shellfish cultivation.
Embodiment 4
The method for carrying out the postoperative low-gravity seawater recuperation of pteria martensii using outdoor sink
(1)The preparation in recuperation pond:Select floor space 30m2, the rectangular outdoor sink of high 1.5m, pond top shelving peace
Dress canvas is taken shelter from rain with shading, per 3m2If gas exhausting stone one, gas exhausting stone 10 is installed altogether, is rinsed well with fresh water and dries 2 days, pond
The strong chlorine oil that the installations and facilities such as son, the gentle stone of tracheae are 25mg/L with available chlorine content(Sym-closene)Carry out full pond disinfection
Processing, then rinsed well repeatedly with filtering sea.
(2)The preparation of low-gravity seawater:The natural sea-water that filling is filtered through husky well and sand filter in clean pond, addition
Well water is configured to the low-gravity seawater of proportion 1.016, pH value 7.8.
(3)Recuperation is configured with water:By step(2)The recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well
In, the depth of water keeps 1.3m, is in boiling-like when water body is inflated;
(4)Plant core operation:Select operate female shellfish, 1.5 age shellfishes of 2 age health pteria martensiis to make piece shell, according to a conventional method into
Row plants core, and operation shellfish presses the density per 35, cage, and right shell is closely packed in upwards in recuperation cage, moves on to step(3)Inject low-gravity sea
Hanging culture in the recuperation pond of water, for hanging culture depth from bottom of pond 20cm, hanging culture density is every cube of 3 cage of water body(105/m3).
(5)The management of pearly shellfish:Shellfish of performing the operation checks once for every 2 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days,
Water is not changed, the gentamicin sulphate and Cefaclor of 0.5mg/L is used alternatingly(A kind of antibiotic be used continuously change again within 3 days it is another
Kind)And the moroxydine of 0.5mg/L.P latymonas subcordifomis 10000/mL.d is fed after 6th day, is started to change water after the 6th day, be changed day
Water 1/5 feeds 20000 cells of P latymonas subcordifomis amount/mL.d after the 10th day, the pearly shellfish recuperation time is 22 days, and pearly shellfish moves
It is cultivated first 3 days to sea area and cultivation is changed to natural sea-water with low-gravity seawater, other management measures do not change, then according to normal
Rule method moves on to sea area and carries out pearly shellfish cultivation.
Embodiment 5
The method for carrying out the postoperative low-gravity seawater recuperation of pteria martensii using outdoor sink
(1)The preparation in recuperation pond:Select floor space 40m2, the round outdoor sink of high 1.35m, pond top shelving peace
Dress canvas is taken shelter from rain with shading, per 4m2If gas exhausting stone one, gas exhausting stone 10 is installed altogether, is rinsed well with fresh water and dries 3 days, pond
The strong chlorine oil that the installations and facilities such as son, the gentle stone of tracheae are 27.5mg/L with available chlorine content(Sym-closene)Full pond is carried out to disappear
Poison processing, then rinsed well repeatedly with filtering sea.
(2)The preparation of low-gravity seawater:The natural sea-water that filling is filtered through husky well and sand filter in clean pond, addition
Well water is configured to the low-gravity seawater of proportion 1.017, pH value 8.05.
(3)Recuperation is configured with water:By step(2)The recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well
In, the depth of water keeps 1.3m, is in boiling-like when water body is inflated;
(4)Plant core operation:Operate female shellfish, 1.25 age shellfishes of 1.75 age health pteria martensiis are selected to make piece shell, routinely side
Method carries out plant core, and operation shellfish presses the density per 37, cage, and right shell is closely packed in upwards in recuperation cage, moves on to step(3)Inject low ratio
Hanging culture in the recuperation pond of weight seawater, for hanging culture depth from bottom of pond 25cm, hanging culture density is every cube of 3 cage of water body(110/m3).
(5)The management of pearly shellfish:Shellfish of performing the operation checks once for every 2.5 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days
Material, does not change water, the gentamicin sulphate and Cefaclor of 0.75mg/L is used alternatingly(A kind of antibiotic is used continuously 3 days and changes again
It is another)And the moroxydine of 0.5mg/L, it feeds after the 6th day and is cultivated in through the filtered natural sea-water of 300 mesh screens on a small quantity
15000 cells of natural algae/mL.d, start to change water after the 6th day, day quantity of exchanged water fed through 300 mesh after the 1/5, the 10th day
30000 cells of the natural algae/mL.d cultivated in natural sea-water after screen filtration, the pearly shellfish recuperation time is 23 days, is educated
Pearl shellfish moves on to sea area cultivation and cultivation was changed to natural sea-water with low-gravity seawater in first 3 days, other management measures do not change, then
It conventionally moves on to sea area and carries out pearly shellfish cultivation.
Embodiment 6
The method for carrying out the postoperative low-gravity seawater recuperation of pteria martensii using outdoor sink
(1)The preparation in recuperation pond:Select floor space about 50m2(Radius 4m, floor space 50.24m2)It is high by 1., the outdoor circle of 3 m
Shape pond, pond top shelving installation canvas are taken shelter from rain with shading.Water inlet pipe, the installation draining of pond center are installed in pool side
Pipe, per 4m2If gas exhausting stone one, 12, gas stone is installed altogether, facilities, the equipment such as pond, the gentle stone of tracheae are rinsed well with fresh water
It dries 3 days, is handled with 50mg/L disinfecting solution of potassium permanganate, then rinsed well repeatedly with filtering sea.
(2)The preparation of low-gravity seawater:The natural sea-water that filling is filtered through husky well and sand filter in clean pond, addition
Tap water is configured to the low-gravity seawater of proportion 1.018, pH value 8.3.
(3)Recuperation is configured with water:By step(2)The recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well
In, the depth of water keeps 1.3m, is in boiling-like when water body is inflated;
(4)Plant core operation:Operate female shellfish, 1.0 age shellfishes of 1.5 age health pteria martensiis are selected to make piece shell, according to a conventional method
Plant core is carried out, according to shellfish body size, by the density per 38, cage after operation, right shell is closely packed in upwards in recuperation cage, moves on to step
(3)Hanging culture in the recuperation pond of low-gravity seawater is injected, for hanging culture depth from bottom of pond 30cm, hanging culture density is every cube of 3 cage of water body
(114/m3).
(5)Pearly shellfish management:Shellfish of performing the operation checks once for every 3 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days, no
Water is changed, the penicillin streptomycin mixture and gentamicin sulphate of 1.0 mg/L is used alternatingly(A kind of antibiotic is used continuously 3 days again
Change another kind)And 0.5 mg/L moroxydine, 50000 cells of chlorella/mL.d is fed after the 6th day, starts to change after the 6th day
Water, day quantity of exchanged water after the 1/3, the 10th day daily ration, feeding quantity be 80000 cells of chlorella/mL.d, pearly shellfish recuperate the time be 25 days, grow cultured pearls
Shellfish moves on to sea area cultivation and cultivation was changed to natural sea-water with low-gravity seawater in first 3 days, is conventionally moved after recuperation
Pearly shellfish cultivation is carried out to sea area.
The effect of growing cultured pearls of geneva pearl oyster difference recuperation mode is relatively shown in Table one
The effect of growing cultured pearls of one geneva pearl oyster difference of table recuperation mode
Pass through contrast test above, it can be deduced that the postoperative low-gravity seawater recuperation method of core shellfish is planted using pteria martensii and is educated
Pearl substantially increases high-quality pearl rate.
Claims (5)
1. a kind of pteria martensii plants the postoperative low-gravity seawater recuperation method of core shellfish, it is characterised in that:Include the following steps:
(1)The preparation in recuperation pond:Selection is taken shelter from rain with pond in the rectangular or circular cell into pumping equipment or with shading
The outdoor sink of facility, installation gas exhausting stone is in boiling-like when water body being made to inflate, using preceding sterile-processed;
(2)The preparation of low-gravity seawater:Using natural sea-water, adds well water or tap water is configured to proportion as 1.016-
1.018 low-gravity seawater, hydrochloric acid or sodium bicarbonate adjust pH value when acid-base value is excessively high either too low;
(3)Recuperation is configured with water:By step(2)In the recuperation pond that the low-gravity seawater injection disinfection of preparation is rinsed well, water
It is deep to keep 1.2-1.3m, it is in boiling-like when water body is inflated;
(4)Pearly shellfish selects and plants core:The healthy pteria martensii of selection, carries out plant core according to a conventional method:According to shellfish body size, hand
The postoperative density by per cage 35-38 only, right shell are closely packed in upwards in recuperation cage, move on to step(3)Middle injection low-gravity seawater
Recuperation is recuperated in pond;
(5)The recuperation management of pearly shellfish:Shellfish of performing the operation checked once per 2-3 days, it is found that dead shellfish is taken out immediately, do not feed bait within first 6 days
Material, does not change water, keeps continuous charge state, antibiotic and moroxydine is used alternatingly, fed on a small quantity after the 6th day P latymonas subcordifomis,
Chlorella and the natural algae cultivated in through the filtered natural sea-water of 300 mesh screens start to change water after the 6th day, change day
Water is the 1/5-1/3 of raw water amount;With the recovery of shellfish body, P latymonas subcordifomis is gradually increased after the 10th day, chlorella, is naturally mixed
The feeding volume of algae is closed, the pearly shellfish recuperation time is 22-25 days, and pearly shellfish moves on to sea area and cultivates preceding 3 days low-gravities by cultivation
Seawater is changed to natural sea-water, and sea area is conventionally moved on to after recuperation and carries out pearly shellfish cultivation.
2. pteria martensii plants the postoperative low-gravity seawater recuperation method of core shellfish according to claim 1, it is characterised in that:Step
(1)It is 20-40 m that the recuperation pond, which selects to have into pumping equipment, high 1.2-1.5m, floor space,2Indoor pond selection
Or floor space is 30-50 m2Outdoor sink, shape be it is rectangular or round, outdoor sink need shelving installation canvas or
Person's colour bar cloth is taken shelter from rain with shading, and pond is per 3-4m2If gas exhausting stone one, water body is in boiling-like when inflation, and oxygen is provided for pearly shellfish
Gas, the breeding facilities such as pond, the gentle stone of tracheae, which are rinsed well before using with fresh water, to be dried 2-3 days, with available chlorine content 25-
The chlorine-containing disinfectant or 30-50mg/L disinfecting solution of potassium permanganate of 30mg/L is handled, then clean with filtering seawater flushing.
3. pteria martensii plants the postoperative low-gravity seawater recuperation method of core shellfish according to claim 1, it is characterised in that:Step
(2)The preparation of the low-gravity seawater is the natural sea-water for extracting sea area from husky filter well with water pump, filters, add through sand filter
Well water either tap water be configured to proportion be 1.016-1.018 low-gravity seawater acid-base value it is excessively high or too low when hydrochloric acid
Or sodium bicarbonate adjusts pH value to 7.8-8.3.
4. pteria martensii plants the postoperative low-gravity seawater recuperation method of core shellfish according to claim 1, it is characterised in that:Step
(4)The shellfish age of the female shellfish of healthy pteria martensii operation is 1.5-2.0 ages, and the shellfish age of piece shell is 1.0-1.5 ages, by normal
Rule method carries out plant core, and according to shellfish body size, by the density per cage 35-38 only after operation, right shell is closely packed in upwards in recuperation cage,
Row's shellfish quantity is booked recuperation cage bottom portion with shellfish body to be advisable, and for hanging culture depth from bottom of pond 20-30cm, hanging culture density is every cube of water body 3
Cage, 105-114/m3。
5. pteria martensii plants the postoperative low-gravity seawater recuperation method of core shellfish according to claim 1, it is characterised in that:Step
(5)The antibiotic of addition is two or more friendships in gentamicin sulphate, Cefaclor, penicillin and streptomysin mixture
For use, a concentration of 0.5mg/L-1.0 mg/L;A concentration of 0.5 mg/L of moroxydine;P latymonas subcordifomis is fed after 6th day
10000-20000 cells/mL.d either chlorella 30000-50000 cells/mL.d or filtered through 300 mesh screens
The natural algae 10000-20000 cells/mL.d cultivated in natural sea-water;With the recovery of shellfish body after 10th day, gradually increase
Add daily ration, feeding quantity, can feed P latymonas subcordifomis 20000-40000 cells/mL.d or chlorella 50000-80000 cells/
ML.d, or mixed algae 20000-40000 cells/mL.d for being cultivated in through the filtered natural sea-water of 300 mesh screens.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111374075A (en) * | 2020-03-20 | 2020-07-07 | 中国水产科学研究院黄海水产研究所 | Method and device for promoting generation of shells of shellfish larvae |
CN112931328A (en) * | 2021-01-29 | 2021-06-11 | 广东海洋大学 | Staged low specific gravity seawater culture method for pinctada martensii during pearl culture |
CN114711175A (en) * | 2022-03-04 | 2022-07-08 | 广东海洋大学 | Method for cultivating black pearls by auto-transplantation of nacre |
CN114831061A (en) * | 2022-05-09 | 2022-08-02 | 广东海洋大学 | Method for recuperating pinctada martensii during pearl culture |
CN115005137A (en) * | 2022-06-01 | 2022-09-06 | 广东海洋大学 | Pinctada martensii pearl culturing method in high-temperature period |
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2018
- 2018-07-17 CN CN201810784993.4A patent/CN108684590B/en active Active
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111374075A (en) * | 2020-03-20 | 2020-07-07 | 中国水产科学研究院黄海水产研究所 | Method and device for promoting generation of shells of shellfish larvae |
CN112931328A (en) * | 2021-01-29 | 2021-06-11 | 广东海洋大学 | Staged low specific gravity seawater culture method for pinctada martensii during pearl culture |
CN114711175A (en) * | 2022-03-04 | 2022-07-08 | 广东海洋大学 | Method for cultivating black pearls by auto-transplantation of nacre |
CN114831061A (en) * | 2022-05-09 | 2022-08-02 | 广东海洋大学 | Method for recuperating pinctada martensii during pearl culture |
CN115005137A (en) * | 2022-06-01 | 2022-09-06 | 广东海洋大学 | Pinctada martensii pearl culturing method in high-temperature period |
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