CN108753654A - A kind of production technology of high activity rhizobium strain and its processing organic fertilizer rich in nitrogen-fixing bacteria - Google Patents

A kind of production technology of high activity rhizobium strain and its processing organic fertilizer rich in nitrogen-fixing bacteria Download PDF

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CN108753654A
CN108753654A CN201810614768.6A CN201810614768A CN108753654A CN 108753654 A CN108753654 A CN 108753654A CN 201810614768 A CN201810614768 A CN 201810614768A CN 108753654 A CN108753654 A CN 108753654A
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傅责中
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Fu Cong
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    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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    • C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • C—CHEMISTRY; METALLURGY
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    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract

The invention discloses the production technologies of a kind of high activity rhizobium strain rich in nitrogen-fixing bacteria and its processing organic fertilizer, it makees strain with the high activity rhizobium composite bacteria rich in nitrogen-fixing bacteria, by various bio-wastes and feces of livestock and poultry, ferment through micro- oxygen consumption, then through be dehydrated, pelletize (or crush) pure organic fertilizer (or organic-biological compound fertilizer) is made；Since fixed nitrogen bacterial content is higher in root nodule flora, bioactivity is strong, under the conditions of half oxygen consumption, micro- oxygen consumption, it cooperates with saccharomycete, rhizopus, basidiomycetes, coordinate jointly with protein therein, hydrolase, invertase, cellulase, lignoenzyme, bio-waste can significantly be converted to nitrate nitrogen, ammoniacal nitrogen, organic acid, organic matter, become the nutrient of nitrogen, phosphorus, potassium substance necessary to farm-forestry crop, industrial crops, preferably solve the Utilizing question of various bio-wastes.

Description

A kind of production of high activity rhizobium strain and its processing organic fertilizer rich in nitrogen-fixing bacteria Technique
Technical field
The present invention relates to the production technologies of a kind of high activity rhizobium strain rich in nitrogen-fixing bacteria and its processing organic fertilizer, belong to In microbial fermentation fertilizer technology field.
Background technology
Biological organic fertilizer is a kind of using microorganisms with specific functions and gas chromatography as raw material, by harmless treatment, corruption The one kind being combined after ripe has both the fertilizer of microbial manure and organic fertilizer effect, and feature is that nutrient is complete, after Can improve soil physical chemistry shape, enhancing soil water-retaining, fertilizer conservation, the ability of fertilizer, the beneficial microbe contained enter after soil with Soil Microorganism forms mutual symbiosis and grows relationship, inhibits harmful bacteria to grow and be converted into beneficial bacterium, plays the association of group Same-action, beneficial bacterium generate a large amount of metabolite during growth and breeding, promote the decomposition and inversion of organic matter, can directly or Between be connected in crop a variety of nutrition and pungent be provided, promote and regulation and control plant growth.
Number of patent application ZL02113756.0 discloses a kind of production for processing organic-biological compound fertilizer with bio-waste Technique, inventor Fu passes through in blaming constantly to grope to improve up to 16 years, it is understood that the patented technology itself is big due to only using Beans root nodule bacteroid makees strain, makes the technology that can only preferably reduce protein content high bio-waste as main nitrogen Source, and to containing a large amount of cellulosics in industrial and agricultural production waste, even contained in the waste containing a large amount of lignin Nutritional ingredient is not fully utilized, and due to the defect of the patented technology, is unfavorable for various bio-wastes (containing livestock and poultry significantly Excrement) it is processed into pure organic fertilizer (or organic-biological compound fertilizer).Therefore, it is necessary to be improved to original patented technology.
Invention content
The invention solves technological improvement problem be：There is provided a kind of high activity rhizobium strain rich in nitrogen-fixing bacteria and its The production technology of organic fertilizer is processed, fixed nitrogen bacterial content is higher in root nodule flora, and bioactivity is strong, in half oxygen consumption, micro- oxygen consumption condition Under, and under the action of microbial life activator, root nodule flora of the high activity rich in nitrogen-fixing bacteria and high-activity yeast bacterium, root Mould, basidiomycetes cooperate, and coordinate jointly with protein therein, hydrolase, invertase, cellulase, lignoenzyme, will Bio-waste is significantly converted into nitrate nitrogen, ammoniacal nitrogen, organic acid, organic matter, become farm-forestry crop, industrial crops institute it is required Nitrogen, phosphorus, potassium substance nutrient, preferably solve the Utilizing question of various bio-wastes.
The technical scheme is that：A kind of high activity rhizobium composite bacteria rich in nitrogen-fixing bacteria, production technology packet Include following steps：
One, strain prepares
A, the preparation of the high activity rhizobium first class inoculum rich in nitrogen-fixing bacteria
First class inoculum to produce high activity rhizobium of the 3kg rich in nitrogen-fixing bacteria calculates, and takes the rhizobium rich in nitrogen-fixing bacteria 100g adds the analysis for soybean powder for accounting for 3kg total amounts 20%, 2% powdered glucose, 10% pig blood solid clot, 10% thin wheat Bran, 2%0Magnesium sulfate, 2%0Ammonium molybdate, 2%0Ferrous sulfate, 2%0Magnesium sulfate, 2%00Manganese sulfate, 2%000Sulphur Sour zinc and 10% microbial life activator stir, and are distributed into container, after being fully sealed, use vacuum pump Until tainer air is extracted into 30%, it is subsequently placed under the conditions of the pH value of 30 DEG C and nature and ferments 148 hours to get this It is for use to stop fermentation by strain high activity first class inoculum 3kg；
B, the preparation of the rhizopus strain of high activity cellulase
Rhizopus strain to produce 325g high activity cellulases calculates, and 19.5g rhizopus strains, addition is taken to account for The powdered glucose of 325g total amounts 5%, 20% starch, 20% thin wheat bran, 4% fine rice bran, then add 3%0Ammonium sulfate, 1%0 Potassium dihydrogen phosphate, 0.5%0Sodium chloride, 0.5%0Ferrous sulfate, 0.5%0Magnesium sulfate, 10% biological life activation Agent and 35% sterile water, stir, ferment 96 hours under the conditions of PH4.5,45 DEG C, obtain the height of 325g The rhizopus strain of active cellulase stops for use after fermenting；
C, the preparation of high activity Saccharomyces cerevisiae
To produce the calculating of 300g high activity Saccharomyces cerevisiaes, 18g Saccharomyces cerevisiaes are taken, the glucose for accounting for 300g total amounts 3% is added Powder, 15% starch, 15% flour, 12% thin wheat bran, 3%0Ammonium sulfate, 0.5%0Sodium chloride, 0.5%0Sulfuric acid Ferrous iron, 0.5%0Magnesium sulfate, 10% biological life activator and 39% sterile water, stir, PH5.0, It under the conditions of 40 DEG C, ferments 96 hours, obtains high activity Saccharomyces cerevisiae 300g, stop for use after fermenting；
Two, the preparation of the high activity rhizobium compound bacteria second class inoculum rich in nitrogen-fixing bacteria
To produce the calculating of 90kg second class inoculums, takes and account for the high activity rhizobium first class inoculum that 75kg is rich in nitrogen-fixing bacteria, 7.5kg High activity Saccharomyces cerevisiae, the rhizopus of 7.5kg high activity cellulases, in addition the powdered glucose of 90kg total amounts 2.6% is accounted for, 4.2% starch, 3.6% analysis for soybean powder, 2.6% pig blood block, 23% wheat bran, 24% meter of sugar, 2%0Ammonium molybdate, 2.7%0Magnesium sulfate, 2.7%0Ferrous sulfate, 3.3%0Ammonium sulfate, 1.1%0Potassium dihydrogen phosphate, 0.5%0Sodium chloride, 0.33%0Manganese sulfate, 0.34%0Zinc sulfate, 4%0Microbial life activator, 4.7% " be rich in CTP aqueous solutions ", And 32% sterile water, stir, under the conditions of PH5,35 DEG C, containing 70% air capacity semitight ferment 146 hours, It obtains and is rich in nitrogen-fixing rhizobia, the high activity compound bacteria second class inoculum of Saccharomyces cerevisiae stops for use after fermenting；
Three, the preparation of the high activity rhizobium compound bacteria three-class strain rich in nitrogen-fixing bacteria
To produce 2.7 tons of three-class strain calculating, 4902kg is taken to be rich in the high activity rhizobium compound bacteria two level bacterium of nitrogen-fixing bacteria Kind, the maize flour of 2.7 tons of total amounts 7.4% of addition, 6% bean cake powder, 24% wheat bran, 25% rice bran add 2%000Molybdenum Sour ammonium, 2.5%000Magnesium sulfate, 2.5%000Ferrous sulfate, 3%000Ammonium sulfate, 1%000Potassium dihydrogen phosphate, 0.5%000's Sodium chloride, the manganese sulfate of 2PPM, the zinc sulfate of .024PPM, 5% " be rich in CTP aqueous solutions " and 20% water, PH5, Hermetically sealed fermentation 168 hours, obtain 2.7 tons of the high activity rhizobium compound bacteria three-level bacterium rich in nitrogen-fixing bacteria under the conditions of 30 DEG C Kind, that is, it is rich in the high activity rhizobium composite bacteria of nitrogen-fixing bacteria, is stopped for use after fermenting.
The microbial life activator is by accounting for the aqueous solution of the CTP of total amount 20%, 75% Ca2+The aqueous solution of CaM It is formed with the aqueous solution mixed preparing of 5% CAMP.
The present invention also provides a kind of, and the high activity root nodule compound bacteria rich in nitrogen-fixing bacteria adds the strain of basidiomycetes, production technology Include the following steps：
A, the preparation of high activity basidiomycetes 1-3 grades of strain
To produce the calculating of 21.7g high activity basidiomycetes first class inoculums, 21.7g basidiomycetes is taken, it is thin that 3.3g glucose, 2g is added Rice bran, 2.2g microbial life activator, 0.11g vitaminsB1, the Oak Tree leaf juice 4.4g of concentration 10% adds 0.07g sulfuric acid Ammonium, 0.02g potassium dihydrogen phosphates, 0.01g sodium chloride, 0.01g ferrous sulfate, 0.01g magnesium sulfate, 0.0044g manganese sulfates, 0.00044g zinc sulfate, semitight fermentation 36 hours, obtain the high activity rich in lignoenzyme under the conditions of PH6.5,40 DEG C Basidiomycetes level-one kind 21.7g, then it is incremented by quantity multiple and same fermentation condition by same formula rate, it is repeated twice, you can system The second class inoculum and three-class strain of the strain are obtained, is stopped for use after fermenting；
B, high activity root nodule compound bacteria adds the preparation of basidiomycetes
Take the high activity rhizobium compound bacteria three-class strain and 1.8 tons of height that nitrogen-fixing bacteria are rich in described in 2.7 tons of claims 1 Active basidiomycetes three-level kind stirs, and mixed fermentation 96 hours to 120 hours under the conditions of semitight obtain 4.5 tons The strain adds basidiomycetes Mixed Microbes three-class strain, that is, the high activity root nodule compound bacteria for being rich in nitrogen-fixing bacteria adds the strain of basidiomycetes.
The present invention also provides a kind of high activity rhizobium compound bacteria fertilizer production kind rich in nitrogen-fixing bacteria, production technology packet Include following steps：It is calculated with producing 81 tons of productions kind, the high activity root rich in nitrogen-fixing bacteria that takes 902.7kg described in claim 1 Tumor bacterium compound bacteria three-class strain is added and accounts for the dregs of beans of 81 tons of total amounts 10%, 12% maize flour, 20% rice bran, 18% wheat bran, The ammonium molybdate of 2PPM, the magnesium sulfate of 2.5PPM, the ferrous sulfate of 2.5PPM, the potassium dihydrogen sulfate of 0.2PPM, the chlorination of 0.5PPM Sodium, 3%0" be rich in CTP aqueous solutions " and 40% water, stir, the hermetically sealed fermentation of room temperature under original pH condition It 168 hours, waits for that fermentate heap is sent out when fermented soya bean and sweet wine mixing smell and raw material are in isabelline and stops fermentation, that is, be made 81 The production kind of the ton composite bacteria.
The present invention also provides a kind of, and the high activity root nodule compound bacteria rich in nitrogen-fixing bacteria adds basidiomycetes fertilizer production kind, production Technique includes the following steps：It is calculated with producing 81 tons of productions kind, takes the height rich in nitrogen-fixing bacteria described in 4.5 tons of claims 3 living Property rhizobium compound bacteria add the three-class strain of basidiomycetes, be added the maize flour for accounting for 81 tons of total amounts 10%, 6% dregs of beans, 24% Wheat bran, 35% rice bran, 15% water, 3%0" be rich in CTP aqueous solutions " and 2PPM ammonium molybdate, the magnesium sulfate of 2.5PPM, The ferrous sulfate of 2.5PPM, the potassium dihydrogen sulfate of 0.2PPM, the sodium chloride of 0.5PPM stir, in original pH condition It is most sealed by fermentation under normal temperature condition 168 hours, waits for that fermentate heap sends out Fermented Soybean and light sweet wine mixing smell, and long on surface Go out canescence mycoderm, inside become it is isabelline to brown when, stop fermentation, that is, the high activity root nodule that the strain adds basidiomycetes be made 81 tons of Mixed Microbes production kind.
The present invention also provides a kind of pure organic bio-fertilizer rich in nitrogen-fixing bacteria, production technology includes the following steps：Take 81 The high activity rhizobium compound bacteria fertilizer production kind and 2430 tons of moisture content≤50% rich in nitrogen-fixing bacteria described in ton claim 4 Bio-waste, be pushed into after stirring in hermetically sealed fermentation tank, be sealed by fermentation 168 hours, it is light to wait for that material has been sent out Fermented soya bean and sweet wine mixing smell, material become isabelline to brown, and start generate ammonia odor when, terminate fermentation, that is, be made 2430 tons, the pure organic bio-fertilizer rich in nitrogen-fixing bacteria of moisture≤40%.The mechanical airing of≤60 DEG C of temperature is used after taking-up, or With the mechanical three tier structure grain of large size granulation, you can the pure biological organic fertilizer rich in nitrogen-fixing bacteria is made.
The present invention also provides a kind of organic-biological compound fertilizer rich in nitrogen-fixing bacteria, production technology includes the following steps：With 1458 tons of the various water feces of livestock and poultry of moisture content >=70% plus 972 tons be ground into particle and after being dehydrated moisture content≤15% it is each Kind crops water suction raw material, in addition the high activity root nodule compound bacteria rich in nitrogen-fixing bacteria described in 81 tons of claims 5 adds basidiomycetes Fertilizer production kind is pushed into after stirring in the sealing and fermenting slot of seal degree >=70%, agripast cloth in covering, hair Ferment 10 to 15 days waits for that material sends out light sweet wine taste and the rotten gas of bean product, and isabelline grows canescence bacterium to brown and surface Stop fermentation when film, 6%-8% calcium magnesium phosphate is added, semitight is fermented 7-8 days, after terminating fermentation, is spread out under the conditions of≤60 DEG C Dry in the air to moisture content≤40%, be eventually adding the urea of 3%-5%, the potassium sulfate of 2%-3%, granulation, moisture content≤30% The organic-biological compound fertilizer rich in nitrogen-fixing bacteria.
The beneficial effects of the invention are as follows：Compared with the prior art, the advantages of the present invention are as follows：
1, the present invention can raw rhizobium differentiate nitrogen-fixing bacteria from chromosome morphology slowly by soybean, and make soybean nodulation bacteroid In contained nitrogen-fixing bacteria improved from less than the breeding of 1% rapid expansion to 20% or so.Nitrogen-fixing bacteria expanding propagation, except to temperature, pH value, The conventional of moisture needs outer, requires micro- oxygen consumption condition particularly severe, and the air capacity in container is only 30% after measured, it is oxygen-containing only When being 10%, the Board Lot of nitrogen-fixing bacteria, bioactivity, reproduction speed are ideal, account in root nodule flora not than regular meeting Disconnected to rise, when highest, can rise to 60%.
2, when the ratio shared in root nodule flora when nitrogen-fixing bacteria is higher, the hydrolysis to protein in liquid culture medium, It is daily 5%, to the percent hydrolysis 0.5% of the protein in solid medium.Its percent hydrolysis to protein, considerably beyond withered grass The microorganisms such as bacillus, aspergillus oryzae, aspergillus niger.
3, under the conditions of half oxygen consumption, micro- oxygen consumption, rhizobium and saccharomycete, rhizopus, basidiomycetes cooperate, and therein Protein, hydrolase, invertase, cellulase, lignoenzyme coordinate jointly, decompose jointly contained in various bio-wastes Discarded high content of protein matter, starch, sugar, cellulose, lignin, even their chelating object, become high level Nitre state ammonia, ammoniacal nitrogen, organic acid, organic matter become the nutrient of nitrogen, phosphorus, potassium substance necessary to farm-forestry crop, industrial crops, Preferably solves the Utilizing question of various bio-wastes, to which the production cost of organic fertilizer, and energy not only be greatly lowered High-volume recycles various bio-wastes.
4, N-P-K content is quite high in the pure organic fertilizer of the present invention, while nitrogen-fixing bacteria are higher in root nodule flora, bioactivity By force, while in organic-biological compound fertilizer inorganic N-P-K content is reasonable, thus agriculture, woods, industrial crops production can be made to be not required to separately Reprocessing, directly in field, forest land is largely applied, and since the organic matter material price of both fertilizer is extremely cheap, carrys out source electrode It is wide extensive, and production cost substantially reduces, and disclosure satisfy that agricultural, industrial crops to fixed nitrogen bacterial content, strong pure of content high activity Organic fertilizer and the large batch of demand of organic-biological compound fertilizer.
By the effort of applicant, the producer of the above-mentioned pure organic fertilizer of large-scale production at present has extended to Liang Jia, total output Be more than ton up to ten thousand, the pure organic fertilizer that is produced institute is nitrogenous, phosphorus, potassium total nutrient have reached 8 to 10, organic matter also reach 60% to 85%, and the content of heavy metal is also down to the 10% to 14% of state quota, currently expand the quantity of large-scale production with Quality fully demonstrates various advantages of this new patent than original ZL02113756.0.At present with aqueous 70% or more chicken manure The trial-production for making organic-biological compound fertilizer has succeeded, and is detected through authoritative institution, N, P2O5、K2The content of O respectively reaches 1.55%, 1.50% and 2.13%.
Specific implementation mode
Invention is described further with reference to specific embodiment：
Material：Microbial life activator, root nodule thalline and " be rich in CTP aqueous solutions ".
Wherein, microbial life activator is by CTP aqueous solutions, Ca2+CaM aqueous solutions and CAMP aqueous solutions mixed preparing and At the water-soluble liquid hold-ups of wherein CTP account for 20%, Ca2+The water-soluble liquid hold-ups of CaM account for the water-soluble liquid hold-ups of 75%, CAMP and account for 5%.CTP and Ca2+What ZL201010216261.9 patented technologies obtained during CaM is blamed by Fu is rich in the compound of CTP, calcium ion and calmodulin Object (Ca2+CaM) stoste of aqueous solution through primary distillation and is evaporated under reduced pressure isolated, and wherein primary distillation is by its in stoste Excess matter removes, and is evaporated under reduced pressure CTP and Ca2+CaM is separated.CAMP (ring-adenosine phosphate) is that product is purchased in market.
The production method of root nodule thalline is：Soybean nodulation bacteroid is disposed into surface silt, weighs up 6.58g, use is miniature Pulverizer crushes, then 154g mixed raw materials are made in 46.2g soy meals, 61.6g bean cake powders, 46.2g fine rice brans, adds 7.65g " being rich in CTP aqueous solutions ", 30g aqua sterilisas, stir evenly, solid medium are made, then by the soybean nodulation after crushing Bacteroid is uniformly admixed in culture medium, pH6--7,30 DEG C -- under the conditions of 35 DEG C semitight ferment 120 hours, 198g mono- is made Grade strain.It is done repeatedly twice again in above-mentioned strain, raw material, activator, the ratio of water and production method, 179kg Soybean Roots is made Tumor bacteroid three-level thalline, for use.
" being rich in CTP aqueous solutions " is by the patent No.：ZL 201010216261.9, patent name be it is a kind of rich in CTP, calcium from The stoste that the method for the production technology of the compound water solution of son and calmodulin is made (is rich in CTP, calcium ion and calcium tune Compound (the Ca of albumen2+CaM) the stoste of aqueous solution) in i.e. containing largely be rich in CTP aqueous solutions.
Embodiment 1：The preparation of high activity rhizobium first class inoculum rich in nitrogen-fixing bacteria
With the rhizobium 10g rich in nitrogen-fixing bacteria, the level-one kind of the high activity of 300g this kinds is produced：This kind of rhizobium are taken first Kind 10g, then 60g analysis for soybean powder, 6g powdered glucoses, 30g pig blood solid blocks are taken, the thin wheat bran of 30g adds 0.6g ammonium molybdates, 0.6g Ferrous sulfate, 0.6g magnesium sulfate, 0.06g manganese sulfates, 0.006g zinc sulfate add 30g microorganism life activator, fully stir It mixes uniformly, is distributed into the conical flask with rubber stopper, after being fully sealed, air in bottle is extracted into only surplus 30% with vacuum pump, so Be placed under the conditions of the pH value of 30 DEG C and nature, fermentation 148 hours to get to strain high activity first class inoculum 300g, It is for use to stop fermentation.
Embodiment 2：The preparation of the rhizopus strain of high activity cellulase
With head mold strain 1.95g, 1.63g powdered glucoses, 0.39g starch, the thin wheat bran of 0.39g, 1.3g fine rice brans is added, then 0.098g ammonium sulfate, 0.032g potassium dihydrogen phosphates, 0.016g sodium chloride, 0.016g ferrous sulfate, 0.016g magnesium sulfate is added, then 3.25g microbial life activator is added, 11.4g sterile waters stir, and fermentation 96 is small under the conditions of PH4.5,45 DEG C When, the rhizopus strain of 32.5g high activity cellulases is obtained, is stopped for use after fermenting.
Embodiment 3：The preparation of high activity Saccharomyces cerevisiae
With Saccharomyces cerevisiae, 1.8g Saccharomyces cerevisiaes are taken, 9g powdered glucoses, 4.5g starch, 4.5g flour, the thin wheats of 3.6g is added It is sub- to add 0.06g ammonium sulfate, 0.01g potassium dihydrogen phosphates, 0.015g sodium chloride, 0.015g magnesium sulfate, 0.015g sulfuric acid for bran Iron adds 3g microbial life activator, and 11.7g sterile waters stir, under the conditions of PH5,40 DEG C, fermentation 96 Hour, 30g high activity Saccharomyces cerevisiaes are obtained, are stopped for use after fermenting.
Embodiment 4：The preparation of high activity rhizobium compound bacteria second class inoculum rich in nitrogen-fixing bacteria
To produce the calculating of 90kg second class inoculums, takes and account for the high activity rhizobium first class inoculum that 75kg is rich in nitrogen-fixing bacteria, 7.5kg High activity Saccharomyces cerevisiae, the rhizopus of 7.5kg high activity cellulases, in addition the powdered glucose of 90kg total amounts 2.6% is accounted for, 4.2% starch, 3.6% analysis for soybean powder, 2.6% pig blood block, 23% wheat bran, 24% meter of sugar, 2%0Ammonium molybdate, 2.7%0Magnesium sulfate, 2.7%0Ferrous sulfate, 3.3%0Ammonium sulfate, 1.1%0Potassium dihydrogen phosphate, 0.5%0Sodium chloride, 0.33%0Manganese sulfate, 0.34%0Zinc sulfate, 4%0Microbial life activator, 4.7% " be rich in CTP aqueous solutions ", And 32% sterile water, stir, under the conditions of PH5,35 DEG C, containing 70% air capacity semitight ferment 146 hours, It obtains and is rich in nitrogen-fixing rhizobia, the high activity compound bacteria second class inoculum of Saccharomyces cerevisiae stops for use after fermenting；
The 3kg first class inoculums that cultured high activity rhizobium (containing 10% or more nitrogen-fixing bacteria) will be distinguished, taking 2.5kg should The high activity rhizopus of strain and 0.25kg, 0.25kg high activity Saccharomyces cerevisiae level-one kinds, by 30 times of meterings are expanded, respectively 75kg high activities rhizobium, 7.5kg high activity rhizopus, 7.5kg high activities bread mould is female, and total amount is answering for tri- kinds of strains of 90kg Combined bacteria kind, wherein 2.34kg powdered glucoses, 3.78kg starch, 3.24kg analysis for soybean powder, 2.34kg pig bloods block, 20.7kg wheats is added Bran, 21.6kg rice brans, in addition 0.18kg ammonium molybdates, 0.24kg magnesium sulfate, 0.24kg ferrous sulfate, 0.26kg ammonium sulfate, 0.089kg potassium dihydrogen phosphates, 0.045kg sodium chloride, 0.0297kg manganese sulfates, 0.003kg zinc sulfate add the micro- of 0.36kg Biological life activator and 4.23kg " being rich in CTP aqueous solutions " and 28.8kg sterile waters, stir, in PH5,35 DEG C of items Under part, most it is sealed by fermentation 146 hours containing 70% air capacity, obtains the high activity of nitrogen-fixing rhizobia, rhizopus, Saccharomyces cerevisiae Compound bacteria two level kind 90kg stops for use after fermenting.
Embodiment 5：The preparation of high activity rhizobium compound bacteria three-class strain rich in nitrogen-fixing bacteria
Take out produced high activity be rich in nitrogen-fixing bacteria rhizobium compound bacteria two level kind 90kg, be added 0.2 ton of maize flour, 0.16 ton of bean cake powder, 0.65 ton of wheat bran, 0.68 ton of rice bran, add 54g ammonium molybdates, 67.5g magnesium sulfate, 67.5g ferrous sulfate, 81g ammonium sulfate, 27g potassium dihydrogen phosphates, 13.5g sodium chloride, 5.4g manganese sulfates, 0.54g zinc sulfate, add 135kg and " are rich in CTP aqueous solutions " and 540kg water, hermetically sealed ferment 168 hours is rich in get the high activity to 2.7 tons under the conditions of PH5,30 DEG C It is for use to stop fermentation for the rhizobium compound bacteria three-level kind of nitrogen-fixing bacteria.
Embodiment 6：The preparation of 1-3 grades of strains of high activity basidiomycetes
12.05g basidiomycetes original species are obtained in completely rotten Oak Tree rotten wood, take the original species of 7.3g, and 3.3g is added Glucose, 2g fine rice brans, 2.2g microbial life activator, 0.1g vitamin B1s, the Oak Tree leaf juice 4.4g of concentration 10%, then add Enter 0.07g ammonium sulfate, 0.02g potassium dihydrogen phosphates, 0.01g sodium chloride, 0.01g ferrous sulfate, 0.01g magnesium sulfate, 0.004g sulphur Sour manganese, 0.004g zinc sulfate, under the conditions of PH6.5,40 DEG C, semitight is fermented 96 hours, and the list rich in lignoenzyme is obtained Daughter bacteria kind level-one kind 219g, then it is incremented by quantity and multiple by same formula rate, it is repeated twice, you can the strain is made, It is for use to stop fermentation for 6.57kg two levels kind and 19.7kg three-level kinds.If needs of production, with first 96 hours in advance, use Same method tries again, you can obtains the production kind of the 591kg strains.
Embodiment 7：High activity root nodule compound bacteria adds the preparation of basidiomycetes
Produced 270kg is rich in the high activity root nodule compound bacteria three-level kind of nitrogen-fixing bacteria, in addition high activity above-mentioned is carried on a shoulder pole Daughter bacteria three-level kind 180kg, stirs, mixed fermentation 96 hours to 120 hours, that is, be made under the conditions of semitight The 450kg strains add load strain mixing three-class strain, are machined with for the water excrement (moisture content >=70%) of various feces of livestock and poultry Machine biological compound fertilizer uses.
Embodiment 8：The preparation of high activity rhizobium compound bacteria fertilizer production kind rich in nitrogen-fixing bacteria
By the 8.1 tons of calculating of production composite bacteria, the high activity root nodule rich in nitrogen-fixing bacteria described in 902.7kg embodiments 5 is taken Bacterium compound bacteria three-class strain is added 810kg dregs of beans, while 972kg maize flours, 162kg rice brans, 146kg wheat bran is added, and adds 16.2 ammonium molybdates, 20.2g magnesium sulfate, 20.2g ferrous sulfate, 1.62 manganese sulfates, 0.16g zinc sulfate, add 24.3g sulfuric acid Ammonium, 8.1g potassium dihydrogen phosphates, 4g sodium chloride add 24.3kg " being rich in CTP aqueous solutions " and 3.24 tons of water, are sufficiently stirred Even, under conditions of original pH, the hermetically sealed fermentation of room temperature 168 hours waits for that fermentate heap sends out Fermented Soybean and sweet wine mixing smell, And raw material stops fermentation when tentatively blacking (or isabelline), that is, the production kind of 8.1 this kind of composite bacteria is made, input is pure organic The production of fertilizer.
Embodiment 9：High activity root nodule compound bacteria rich in nitrogen-fixing bacteria adds the preparation of basidiomycetes fertilizer production kind
High activity root nodule compound bacteria adds basidiomycetes 450kg in Example 7, and by 30 times of total amount, 135kg corns are added Face, 81kg dregs of beans, 3.24 tons of wheat bran, 4.7 tons of rice brans, add 2.03 tons of water, and it is former rich in CTP aqueous solutions to add 40.5kg Liquid and 2.7g ammonium molybdates, 33.75g magnesium sulfate, 33.75g ferrous sulfate, 2.7g manganese sulfates, 3.3g zinc sulfate and 40.5g sulphur Sour ammonium, 13.5g potassium dihydrogen phosphates, 6.75g sodium chloride.It stirs, under original pH condition and normal temperature condition is most close Envelope 168 hours waits for that fermentate heap sends out Fermented Soybean and light sweet wine smell, and grows canescence mycoderm on surface, inside become and When yellow to brown, that is, 13.5 tons of the mixture manufacturing kind that the strain adds basidiomycetes is made, you can by various moisture content >=70% Livestock and poultry water excrement, is processed into organic-biological compound fertilizer semi-finished product.
Embodiment 10：The preparation of pure organic bio-fertilizer rich in nitrogen-fixing bacteria
With manufactured 72.9 tons of cigarette waste residue powder (also known as crude opium) after 170 tons of Maotai-flavor distiller's grain meal, useless cigarette foot crushing to 73 tons, With water spray to aqueous 40%, amount to 243 tons.In addition being rich in the high activity rhizobium compound bacteria of nitrogen-fixing bacteria in above-described embodiment 8 8.1 tons of fertilizer production kind is repeatedly turned over throwing, is stirred with big machineries such as agricultural turners, with mainframes such as bull-dozers Tool, push-in can accommodate in 150 tons of the hermetically sealed fermentation tank of salable, controllable air content, are sealed by fermentation 168 hours, wait for Distiller's grain meal and useless cigarette foam soil send out the mixing smell of Fermented Soybean and sweet wine, and start to generate ammonia odor, terminate fermentation, that is, are made The pure organic bio-fertilizer of 243 tons of moisture content≤40%, uses≤60 DEG C of mechanical airing after taking-up, or with large size granulator three-level Granulation, you can the pure organic fertilizer rich in nitrogen-fixing bacteria of moisture content≤30% is made, then with there is inner bag plastic bag sealing to pack, i.e., As finished product, storage is (or use) for sale.
Embodiment 11：The preparation of organic-biological compound fertilizer rich in nitrogen-fixing bacteria
The mixed bacteria of basidiomycetes is added to process various water with the high activity rhizobium compound bacteria for being rich in nitrogen-fixing bacteria in embodiment 9 >=70% water feces of livestock and poultry is divided to process organic-biological compound fertilizer：With 243 tons of the various water feces of livestock and poultry of various moisture content >=70% With by 162 tons of dehydrations, to 15% drier oil vegetable seed below, it is crushed to grain size 4mm to 5mm, becomes water suction raw material, in addition produced 13.5 tons of high activity root nodule compound bacterias rich in nitrogen-fixing bacteria add basidiomycetes hybrid, it is multiple with big machineries such as agricultural turners Throwing is turned over, is stirred, with big machineries such as bull-dozers by rape straw powder and above-mentioned production strain, push-in volume is 250 The seal degree of ton or so is in 70% or so, the fermentation hole that can facilitate disengaging material, agripast cloth in covering, fermentation 10 To 15 days, wait for that material sends out light sweet wine taste and the rotten smell of bean product, and become dark brown, yellowish-brown and surface and grow canescence When mycoderm, then the nutritional need of various crops is pressed in pause fermentation, and 36 tons of calcium magnesium phosphate, semitight fermentation 7-8 is added It, after terminating fermentation, using the mechanical airing of 60 DEG C of < to moisture content≤40%, is eventually adding 13.5 tons of urea, 4.5 tons of sulfuric acid Potassium becomes the organic-biological compound fertilizer rich in nitrogen-fixing bacteria of moisture content≤30%, finally with the mechanical three tier structure grain of large size granulation With there is the plastic bag sealing of inner bag to pack, 315 tons or so of finished product is become, storage is (or use) for sale.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that The specific implementation of the present invention is confined to these explanations.For those of ordinary skill in the art to which the present invention belongs, exist Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to the present invention's Protection domain.

Claims (7)

1. a kind of high activity rhizobium composite bacteria rich in nitrogen-fixing bacteria, which is characterized in that its production technology includes the following steps：
One, strain prepares
A, the preparation of the high activity rhizobium first class inoculum rich in nitrogen-fixing bacteria
First class inoculum to produce high activity rhizobium of the 3kg rich in nitrogen-fixing bacteria calculates, and takes the rhizobium 100g rich in nitrogen-fixing bacteria, Add the analysis for soybean powder for accounting for 3kg total amounts 20%, 2% powdered glucose, 10% pig blood solid clot, 10% thin wheat bran, 2%0Magnesium sulfate, 2%0Ammonium molybdate, 2%0Ferrous sulfate, 2%0Magnesium sulfate, 2%00Manganese sulfate, 2%000Sulfuric acid Zinc and 10% microbial life activator stir, and are distributed into container, will with vacuum pump after being fully sealed Until tainer air is extracted into 30%, it is subsequently placed under the conditions of the pH value of 30 DEG C and nature and ferments 148 hours to get the bacterium Kind high activity first class inoculum 3kg, it is for use to stop fermentation；
B, the preparation of the rhizopus strain of high activity cellulase
Rhizopus strain to produce 325g high activity cellulases calculates, and takes 19.5g rhizopus strains, it is total that addition accounts for 325g The powdered glucose of amount 5%, 20% starch, 20% thin wheat bran, 4% fine rice bran, then add 3%0Ammonium sulfate, 1%0Phosphoric acid Potassium dihydrogen, 0.5%0Sodium chloride, 0.5%0Ferrous sulfate, 0.5%0Magnesium sulfate, 10% biological life activator, and 35% sterile water, stirs, and ferments 96 hours under the conditions of PH4.5,45 DEG C, obtains the high activity of 325g containing fibre The rhizopus strain of the plain enzyme of dimension stops for use after fermenting；
C, the preparation of high activity Saccharomyces cerevisiae
To produce the calculating of 300g high activity Saccharomyces cerevisiaes, 18g Saccharomyces cerevisiaes are taken, the powdered glucose for accounting for 300g total amounts 3% is added, 15% starch, 15% flour, 12% thin wheat bran, 3%0Ammonium sulfate, 0.5%0Sodium chloride, 0.5%0Sulfuric acid it is sub- Iron, 0.5%0Magnesium sulfate, 10% biological life activator and 39% sterile water, stir, in PH5.0,40 It under the conditions of DEG C, ferments 96 hours, obtains high activity Saccharomyces cerevisiae 300g, stop for use after fermenting；
Two, the preparation of the high activity rhizobium compound bacteria second class inoculum rich in nitrogen-fixing bacteria
It to produce the calculating of 90kg second class inoculums, takes and accounts for the high activity rhizobium first class inoculum that 75kg is rich in nitrogen-fixing bacteria, 7.5kg high lives Property Saccharomyces cerevisiae, the rhizopus of 7.5kg high activity cellulases, in addition the powdered glucose of 90kg total amounts 2.6% is accounted for, 4.2% Starch, 3.6% analysis for soybean powder, 2.6% pig blood block, 23% wheat bran, 24% meter of sugar, 2%0Ammonium molybdate, 2.7%0Sulfuric acid Magnesium, 2.7%0Ferrous sulfate, 3.3%0Ammonium sulfate, 1.1%0Potassium dihydrogen phosphate, 0.5%0Sodium chloride, 0.33%0Manganese sulfate, 0.34%0Zinc sulfate, 4%0Microbial life activator, 4.7% " be rich in CTP aqueous solutions " and 32% it is sterile Water stirs, and under the conditions of PH5,35 DEG C, ferments 146 hours, is obtained rich in fixed nitrogen containing 70% air capacity semitight Rhizobium, the high activity compound bacteria second class inoculum of Saccharomyces cerevisiae stop for use after fermenting；
Three, the preparation of the high activity rhizobium compound bacteria three-class strain rich in nitrogen-fixing bacteria
To produce 2.7 tons of three-class strain calculating, takes 4902kg to be rich in the high activity rhizobium compound bacteria second class inoculum of nitrogen-fixing bacteria, add Enter the maize flour of 2.7 tons of total amounts 7.4%, 6% bean cake powder, 24% wheat bran, 25% rice bran adds 2%000Ammonium molybdate, 2.5%000Magnesium sulfate, 2.5%000Ferrous sulfate, 3%000Ammonium sulfate, 1%000Potassium dihydrogen phosphate, 0.5%000Chlorination Sodium, the manganese sulfate of 2PPM, the zinc sulfate of .024PPM, 5% " be rich in CTP aqueous solutions " and 20% water, PH5,30 DEG C Under the conditions of it is hermetically sealed fermentation 168 hours, obtain 2.7 tons of the high activity rhizobium compound bacteria three-class strain rich in nitrogen-fixing bacteria, i.e., High activity rhizobium composite bacteria rich in nitrogen-fixing bacteria stops for use after fermenting.
2. the rhizobium composite bacteria according to claim 1 rich in nitrogen-fixing bacteria, it is characterised in that：The microbial life Activator is by accounting for the aqueous solution of the CTP of total amount 20%, 75% Ca2+The aqueous solution of CaM and the mixing of the aqueous solution of 5% CAMP It is formulated.
3. a kind of high activity root nodule compound bacteria rich in nitrogen-fixing bacteria adds the strain of basidiomycetes, it is characterised in that：Its production technology packet Include following steps：
A, the preparation of high activity basidiomycetes 1-3 grades of strain
To produce the calculating of 21.7g high activity basidiomycetes first class inoculums, 21.7g basidiomycetes is taken, 3.3g glucose, the thin rice of 2g is added Chaff, 2.2g microbial life activator, 0.11g vitaminsB1, the Oak Tree leaf juice 4.4g of concentration 10% adds 0.07g sulfuric acid Ammonium, 0.02g potassium dihydrogen phosphates, 0.01g sodium chloride, 0.01g ferrous sulfate, 0.01g magnesium sulfate, 0.0044g manganese sulfates, 0.00044g zinc sulfate, semitight fermentation 36 hours, obtain the high activity rich in lignoenzyme under the conditions of PH6.5,40 DEG C Basidiomycetes level-one kind 21.7g, then it is incremented by quantity multiple and same fermentation condition by same formula rate, it is repeated twice, you can system The second class inoculum and three-class strain of the strain are obtained, is stopped for use after fermenting；
B, high activity root nodule compound bacteria adds the preparation of basidiomycetes
Take the high activity rhizobium compound bacteria three-class strain and 1.8 tons of high activities that nitrogen-fixing bacteria are rich in described in 2.7 tons of claims 1 Basidiomycetes three-level kind stirs, and mixed fermentation 96 hours to 120 hours under the conditions of semitight obtain 4.5 tons of bacterium Kind plus basidiomycetes Mixed Microbes three-class strain, that is, the high activity root nodule compound bacteria for being rich in nitrogen-fixing bacteria add the strain of basidiomycetes.
4. a kind of high activity rhizobium compound bacteria fertilizer production kind rich in nitrogen-fixing bacteria, it is characterised in that：Its production technology includes Following steps：It is calculated with producing 81 tons of productions kind, the high activity root nodule rich in nitrogen-fixing bacteria that takes 902.7kg described in claim 1 Bacterium compound bacteria three-class strain is added and accounts for the dregs of beans of 81 tons of total amounts 10%, 12% maize flour, 20% rice bran, 18% wheat bran, The ammonium molybdate of 2PPM, the magnesium sulfate of 2.5PPM, the ferrous sulfate of 2.5PPM, the potassium dihydrogen sulfate of 0.2PPM, the chlorination of 0.5PPM Sodium, 3%0" be rich in CTP aqueous solutions " and 40% water, stir, the hermetically sealed fermentation of room temperature under original pH condition It 168 hours, waits for that fermentate heap is sent out when fermented soya bean and sweet wine mixing smell and raw material are in isabelline and stops fermentation, that is, be made 81 The production kind of the ton composite bacteria.
5. a kind of high activity root nodule compound bacteria rich in nitrogen-fixing bacteria adds basidiomycetes fertilizer production kind, it is characterised in that：It produces work Skill includes the following steps：It is calculated with producing 81 tons of productions kind, takes the high activity rhizobium rich in nitrogen-fixing bacteria described in claim 3 Compound bacteria adds 4.5 tons of three-class strains of basidiomycetes, and the maize flour for accounting for 81 tons of total amounts 10%, 6% dregs of beans, 24% wheat is added Bran, 35% rice bran, 15% water, 3%0" be rich in CTP aqueous solutions " and 2PPM ammonium molybdate, the magnesium sulfate of 2.5PPM, The ferrous sulfate of 2.5PPM, the potassium dihydrogen sulfate of 0.2PPM, the sodium chloride of 0.5PPM stir, in original pH condition It is most sealed by fermentation under normal temperature condition 168 hours, waits for that fermentate heap sends out Fermented Soybean and light sweet wine mixing smell, and long on surface Go out canescence mycoderm, inside become it is isabelline to brown when, stop fermentation, that is, the high activity root nodule that the strain adds basidiomycetes be made 81 tons of Mixed Microbes production kind.
6. a kind of pure organic bio-fertilizer rich in nitrogen-fixing bacteria, it is characterised in that：Its production technology includes the following steps：Take 81 tons of power Profit requires the life of high activity rhizobium the compound bacteria fertilizer production kind and 2430 tons of moisture content≤50% rich in nitrogen-fixing bacteria described in 4 Object waste is pushed into after stirring in hermetically sealed fermentation tank, is sealed by fermentation 168 hours, is waited for that material has sent out Fermented Soybean With sweet wine mixing smell, material becomes isabelline to brown, and start generate ammonia odor when, terminate fermentation, that is, be made 2430 tons, The pure organic bio-fertilizer rich in nitrogen-fixing bacteria of moisture≤40%.
7. a kind of organic-biological compound fertilizer rich in nitrogen-fixing bacteria, it is characterised in that：Its production technology includes the following steps：With aqueous Divide >=70% 1458 tons of various agricultures for being ground into moisture content≤15% after particle and dehydration plus 972 tons of various water feces of livestock and poultry Crop water suction raw material, in addition the high activity root nodule compound bacteria rich in nitrogen-fixing bacteria described in 81 tons of claims 5 adds basidiomycetes fertilizer Production kind is pushed into after stirring in the sealing and fermenting slot of seal degree >=70%, agripast cloth in covering, fermentation 10 To 15 days, wait for that material sends out light sweet wine taste and the rotten gas of bean product, and isabelline when growing canescence mycoderm to brown and surface Stop fermentation, 6%-8% calcium magnesium phosphate is added, semitight is fermented 7-8 days, is terminated after fermenting, airing is extremely under the conditions of≤60 DEG C Moisture content≤40%, is eventually adding the urea of 3%-5%, the potassium sulfate of 2%-3%, granulation, moisture content≤30% richness Organic-biological compound fertilizer containing nitrogen-fixing bacteria.
CN201810614768.6A 2018-06-14 2018-06-14 A kind of production technology of high activity rhizobium strain and its processing organic fertilizer rich in nitrogen-fixing bacteria Pending CN108753654A (en)

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CN113402313A (en) * 2021-06-24 2021-09-17 贵州鸿洋生物有机肥有限公司 Cytidine triphosphate biological organic solid fertilizer formula and production method thereof
EP3892716A1 (en) 2020-04-07 2021-10-13 Biofor System d.o.o. Novel plant growth promoting bacterial strains, an improved method for encapsulation of bacteria and a biofertilizer comprising encapsulated bacteria prepared by the said method

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CN101850050A (en) * 2010-07-02 2010-10-06 傅责中 Production process of compound water solution rich in CTP, calcium ions and calmodulin
CN104774054A (en) * 2015-04-28 2015-07-15 永州万喜登农业发展有限公司 Bio-organic fertilizer
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