CN108743457A - A kind of compound essential oil with effect of weight reducing - Google Patents
A kind of compound essential oil with effect of weight reducing Download PDFInfo
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- CN108743457A CN108743457A CN201810630535.5A CN201810630535A CN108743457A CN 108743457 A CN108743457 A CN 108743457A CN 201810630535 A CN201810630535 A CN 201810630535A CN 108743457 A CN108743457 A CN 108743457A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The invention discloses a kind of compound essential oils with effect of weight reducing.Present invention employs the way of distillation be made rosemary leave, dried peppermint leaf and fennel fruit compound essential oil, then use CO2The extraction essential oil of black pepper seed and sweet orange seed has been made in supercritical extract, the two is mixed again, it is still aging at room temperature, then the technique of impurity screening, obtained compound essential oil smell is fresh and clean, pure, and stablize, it is tested through volunteer visible, compound essential oil obtained by the present invention has excellent skin affinity and Percutaneously absorbable and can enhance fat metabolism, and it can be worked by being used as lipolytic agent and treat, reduces or inhibits obesity to enhance the fat metabolism of whole or local subcutaneous adipose tissue.
Description
Technical field
The present invention relates to cosmetic fields, and in particular to a kind of compound essential oil with effect of weight reducing.
Background technology
Plants essential oil be extraction the distinctive aromatic substance of plant, be taken from herbal flower, leaf, root, bark, fruit,
What seed, resin etc. were extract to distill, in a manner of squeezing.Since essential oil-fumigation volatility is high, and molecule is small, it is easy to by people
Body absorbs, and can permeate human skin rapidly, and whole process only needs a few minutes, and the fragrance of plant itself, also directly stimulates
The secretion of pituitary gland, ferment and hormone secretion etc., function in balanced body, play the role of weight-reducing.
Fennel essential oil, the entitled fennel of cosmetics Chinese（FOENICULUM VULGARE）Oil is extracted from fennel fruit
It is made, can improve that skin is slack and undisciplined and pore is coarse, have the function of moisturizing, consolidation skin, remove microgroove, to preventing wrinkle and tangerine
Skin tissue etc. is also very helpful.
Rosemary Oil, the entitled rosemary of cosmetics Chinese（ROSMARINUS OFFICINALIS）Leaf oil, have compared with
Strong astriction can improve greasy unclean skin, promote blood circulation, and mitigate congested, swelling situation, moreover it is possible to improve head
Scurf stimulates hair growth.
Black pepper essential oil, the entitled pepper of cosmetics Chinese（PIPER NIGRUM）Seed oil, clean-up effect is excellent, can improve
The suppuration phenomenon of wound infection.
Peppermint essential oil, the entitled peppermint of cosmetics Chinese（MENTHA HAPLOCALYX）Oil extracts system from dried peppermint leaf
, when hot can it is refrigerant, cold when then can warm body, relieveing pain and refreshing, body and mind of releiving, have shrink capilary, releive itch, inflammation
With burn, the skin care effect of soft skin.
Sweet orange oil, the entitled sweet orange of cosmetics Chinese（CITRUS AURANTIUM DULCIS）Seed oil, has guarantor to skin
Wet effect, the pH-value of energy balance skin, help collagen are formed, and have good effect for the growth and reparation of skin histology.
The plants essential oil that the prior art provides all is the folk prescription essential oil extracted as raw material using a certain plant, i.e., is planted from one kind
The whole strain of object or some position purification by liquid extraction and obtain, then further according to demand by different folk prescription essential oils according to a certain percentage
Compound essential oil is made in compounding together, however, that there are fragrance is not pure enough, is easy to become for compound essential oil obtained according to the method described above
The problems such as discoloration matter.
Invention content
The purpose of the present invention is overcoming above-mentioned technical problem, provide that a kind of stability is good, the good compound essence of fat-reducing effect
Oily preparation method.
Meanwhile the present invention also provides a kind of compound essential oils prepared using the above method.
Technical scheme is as follows：
A kind of preparation method of compound essential oil, includes the following steps：
Step 1: rosemary leave, dried peppermint leaf are dried in the shade indoors；
Step 2: using rosemary leave, dried peppermint leaf and fennel fruit as raw material, raw material is subjected to infiltration and ultrasonication, then
Distillation essential oil is obtained using the method for distillation extraction again, wherein
The infiltration is specially：In rosemary leave, dried peppermint leaf and fennel fruit input distillation still that step 1 is obtained, addition is gone
Ionized water impregnates, wherein the deionized water weight is 5 ~ 10 times of the sum of rosemary leave, dried peppermint leaf and fennel fruit weight, leaching
Interruption is mixed slowly during bubble, 10 ~ 30 revs/min of mixing speed, until rosemary leave, dried peppermint leaf are infiltrated by deionized water
Fully；
The ultrasonication is specially：Mixing speed is controlled at 10 ~ 50 revs/min, by material heat temperature raising in distillation still
To 80 ~ 100 DEG C, use frequency for the ultrasonication 5 ~ 10 minutes of 30 ~ 100 KHZ；
Step 3: black pepper seed, sweet orange seed are mixed, crushed, CO is taken2The method of supercritical extract obtains extraction essential oil；
Step 4: the distillation essential oil that step 2 obtains and the extraction essential oil that step 3 obtains are mixed, it is still aging at room temperature,
Then the filter of 1 ~ 10 micron pore size is used to filter to get compound essential oil.
Preferably, the rosemary leave, dried peppermint leaf, fennel fruit, black pepper seed, sweet orange seed weight ratio be：1：0.1~
10：0.1~10：0.1~10：0.1~10.
More preferably, the rosemary leave, dried peppermint leaf, fennel fruit, black pepper seed, sweet orange seed weight ratio be：1:
5:2:3:2。
Preferably, in the step 2, the process conditions for distilling extraction are：Vapo(u)rizing temperature is 100 ~ 120 DEG C.
Preferably, in the step 3, CO2The process conditions of supercritical extract are：Pressure is 1 ~ 10MPa in extraction kettle,
45 ~ 65 DEG C of extraction temperature, extraction time 60-80min.
Preferably, in the step 4, digestion time is 5 ~ 10 days.
Present invention simultaneously provides a kind of compound essential oil, the preparation method of the compound essential oil includes the following steps：
Step 1: rosemary leave, dried peppermint leaf are dried in the shade indoors；
Step 2: using rosemary leave, dried peppermint leaf and fennel fruit as raw material, raw material is subjected to infiltration and ultrasonication, then
Distillation essential oil is obtained using the method for distillation extraction again, wherein
The infiltration is specially：In rosemary leave, dried peppermint leaf and fennel fruit input distillation still that step 1 is obtained, addition is gone
Ionized water impregnates, wherein the deionized water weight is 5 ~ 10 times of the sum of rosemary leave, dried peppermint leaf and fennel fruit weight, leaching
Interruption is mixed slowly during bubble, 10 ~ 30 revs/min of mixing speed；
The ultrasonication is specially：Mixing speed is controlled at 10 ~ 50 revs/min, by material heat temperature raising in distillation still
To 80 ~ 100 DEG C, use frequency for the ultrasonication 5 ~ 10 minutes of 30 ~ 100 KHZ；
Step 3: using black pepper seed, sweet orange seed as raw material, CO is taken2The method of supercritical extract obtains extraction essential oil；
Step 4: the distillation essential oil that step 2 obtains and the extraction essential oil that step 3 obtains are mixed, it is still aging at room temperature,
Then the filter of 1 ~ 10 micron pore size is used to filter；
Obtain the compound essential oil.
The above-mentioned compound essential oil of the present invention can be used directly, can also be used as a kind of additive application in weight-reducing skin care item
In, the weight-reducing skin care item are made of above-mentioned compound essential oil and skin care field customary adjuvant.
The present invention also provides a kind of weight-reducing massage oil, include the component of following mass percentage：
Above-mentioned compound essential oil 0.1-10%,
Grape（VITIS VINIFERA）Seed oil 0.1-10%,
Aloe（ALOE VERA）Extract 0.1-10%,
Sweet almond（PRUNUS AMYGDALUS DULCIS）Oily 0.1-10%,
Jojoba（SIMMONDSIA CHINENSIS）Seed oil 60-90%,
Tocopherol acetate 0.1-1%.
The preparation process of weight-reducing massage oil of the present invention is easy, and the component is put into agitated kettle successively, stirs
Uniformly.
Compared to the prior art, compound essential oil provided by the invention and preparation method thereof has the advantages that：
One, present invention employs the way of distillation be made rosemary leave, dried peppermint leaf and fennel fruit compound essential oil, then use CO2It is super
The extraction essential oil of black pepper seed and sweet orange seed has been made in critical extraction, then the two is mixed, still aging at room temperature, then mistake
The technique for filtering impurity, obtained compound essential oil smell is fresh and clean, pure, and stablizes, through volunteer's test as it can be seen that the present invention
Obtained compound essential oil has excellent skin affinity and Percutaneously absorbable and can enhance fat metabolism, and it can
To enhance the fat metabolism of whole or local subcutaneous adipose tissue by working as lipolytic agent treating, reduce or
Inhibit fat.
Two, it before distilling extractive distillation essential oil, is handled by using ultrasonic wave, has been greatly facilitated extraction efficiency, increased
Add the dissolution rate of active constituent.Ultrasonic wave penetrates material in distillation still, reaches the inside of material, when cell interior pressure is more than thin
Cell wall ability to bear, cell rupture, intracellular active ingredient freely flow out so that distillation recovery rate is high and quality is good.
Specific implementation mode
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention will be made into one below
Step ground detailed description, embodiments of the present invention are not limited thereto, for not specifically specified technological parameter or condition, can refer to
Routine techniques carries out.
The present invention provides a kind of preparation method of compound essential oil, includes the following steps：
（1）Pre-treatment step：Rosemary leave, dried peppermint leaf, fennel fruit, black pepper seed, sweet orange seed are weighed, then by rosemary
Leaf, dried peppermint leaf are placed on interior and dry in the shade；
Specifically, suitable rosemary leave, dried peppermint leaf, fennel fruit, black pepper seed, sweet orange seed are weighed, then by rosemary leave,
Dried peppermint leaf is placed on interior and dries in the shade, wherein limit rosemary leave, dried peppermint leaf, fennel fruit, black pepper seed, sweet orange seed weight ratio
It is 1：0.1~10：0.1~10：0.1~10：0.1 ~ 10, more preferably, rosemary leave, dried peppermint leaf, fennel fruit, black pepper seed,
The weight ratio of sweet orange is：1:5:2:3:2.
（2）Using rosemary leave, dried peppermint leaf and fennel fruit as raw material, raw material is subjected to infiltration and ultrasonication, then
Distillation essential oil is obtained using the method for distillation extraction again；
Specifically, described infiltrate is specially：By step（1）Rosemary leave, dried peppermint leaf and fennel fruit input distillation still in, add
Enter deionized water to be impregnated, the deionized water weight is the 5 ~ 10 of the sum of rosemary leave, dried peppermint leaf and fennel fruit weight
Times, interruption is mixed slowly in soaking process, 10 ~ 30 revs/min of mixing speed, until rosemary leave, dried peppermint leaf and fennel fruit
It is real to be infiltrated fully by deionized water；
The ultrasonication is specially：Mixing speed is controlled at 10 ~ 50 revs/min, by material heat temperature raising in distillation still
To 80 ~ 100 DEG C, use frequency for the ultrasonication 5 ~ 10 minutes of 30 ~ 100 KHZ；
After ultrasonication, material in distillation still is heated to 100 ~ 120 DEG C, opens the condenser of distillation still, and cold
Circulate cooling water in condenser, collects distillation essential oil.
（3）Using black pepper seed, sweet orange seed as raw material, CO is taken2The method of supercritical extract obtains extraction essential oil；
Black pepper seed, sweet orange seed are put into supercritical extraction reactor, it is 4MPa, 55 DEG C of temperature, extraction that pressure in extraction kettle, which is arranged,
Time 80min obtains extraction essential oil.
（4）By step（2）The distillation essential oil and step of acquisition（3）The extraction essential oil of acquisition mixes, and stands at room temperature old
Change 10 ~ 20 days, the filter of 1 ~ 10 micron pore size is then used to filter to get compound essential oil.
Preferred embodiment 1
Weigh rosemary leave 10kg, dried peppermint leaf 50kg, fennel fruit 20kg, black pepper seed 30kg, sweet orange seed 20kg, rosemary leave
It spreads out and dries in the shade indoors with dried peppermint leaf；Will rosemary leave, dried peppermint leaf, fennel fruit input distillation still in, be added 1000kg go from
Sub- water is impregnated, and interruption is mixed slowly in soaking process, and 15 revs/min of mixing speed is made by long agitation at a slow speed
It impregnates more abundant；Then mixing speed is controlled at 50 revs/min, material in distillation still is heated to 80 DEG C, using frequency
The ultrasonication that rate is 50 KHZ 5 minutes；After ultrasonication, material in distillation still is heated to 100 DEG C, is opened
The condenser of distillation still, and the cooling water that circulates within the condenser collect distillation essential oil.Black pepper seed, sweet orange seed are put into CO2It is super
In critical extraction kettle, it is 4MPa that pressure in extraction kettle, which is arranged, and 55 DEG C of temperature extracts 80min, obtains extraction essential oil.It will be obtained
Essential oil and extraction essential oil mixing are distilled, still aging 10 days at room temperature, then uses the filter of 10 micron pore sizes to filter, i.e.,
Obtain compound essential oil.
Existing comparative example 1
Commercially available fennel essential oil, Rosemary Oil, black pepper essential oil, Peppermint essential oil, sweet orange oil, according to 1:5:2:3:2 ratio
Example mixing.
Test one, test cell line
Test the compound essential oil obtained by preferred embodiment and existing comparative example.Fat metabolism activity is measured, and by being based on fat
Glycerine and free fatty acids are discharged into the fact in culture medium and quantitative measurment by the metabolism for the fat accumulated in fat cell
Glycerine in culture medium is compared.
By as the mouse 3T3 cell dispersions of preadipocyte on the disk of diameter 10cm so that on the disk there are 1 ×
104Cell.With the DMEM culture mediums (being obtained from Invitrogen) containing 10%FCS in 5%CO2It is carried out at 37 DEG C in atmosphere
Cultivate the cell 1 week.Later, with containing 0.25M dexamethasone, 0.5mM isobutyl methylxanthines and 10 μ g/ml insulin
The DMEM culture mediums containing 10%FCS replace the culture medium, and continue to cultivate other two days.Then, which is used again
Common DMEM culture mediums containing 10%FCS replace, and are additionally carried out culture one week, and it is that fat is thin to lead to preadipocyte differentiation
Born of the same parents.And then, it is replaced with the DMEM culture mediums containing the compound essential oil obtained by 250 μM of preferred embodiments or existing comparative example
The culture medium, and the culture medium is recycled at 24 hours later.Cell is cleaned with Dulbecco ' s PBS (-) and is recycled.It uses
The cell cultivated when in the absence of test substances is as a contrast.With Glycerol Test E Wako (Wako Pure
Chemical Industries, Ltd.) glycerol content in quantitative measurment culture medium.Table 1 is shown to be provided by test substances
, free glycerol content relative to control (100%).
Table 1
The above results show that, relative to reference, administration test substances result in the increase of free glycerol content.Especially, preferably
Embodiment obtains the free glycerine of higher amount, shows that fat metabolism enhances.
Test two, volunteer's test
Volunteer's test is carried out to the compound essential oil obtained by preferred embodiment and existing comparative example.
Test method：Each experimental group is fat volunteer 20（30-50 Sui, men and women is fifty-fifty）；
Test zone：Abdomen area；
It uses：Twice daily, it persistently uses 4 weeks；
Test event：The use of reduced more than 0.5cm or more with stomach fat before and after product is effective；Stomach fat is reduced
0.5cm below is effective；It is invalid that stomach fat, which does not decrease or increase,.
Result on trial see the table below 2.
2 efficacy test record sheet of table
| It is effective | Effectively | In vain | |
| Embodiment 1 | 10 | 6 | 4 |
| Comparative example 1 | 0 | 9 | 11 |
As seen from Table 2, the effect of weight reducing of the compound essential oil obtained by present pre-ferred embodiments is more preferably.
Test three, stability test
Volunteer's test is carried out to the compound essential oil obtained by embodiment 1 and comparative example 1.
Test method：The compound essential oil obtained by embodiment 1 and comparative example 1 is positioned over 45 DEG C, -15 DEG C, room temperature respectively
Under conditions of place 3 months, seen whether the abnormal conditions such as precipitation, discoloration, spoiled.Test result is shown in Table 3.
3 stability test record sheet of table
| 1 month | 2 months | 3 months | |
| Embodiment 1 | It is qualified | It is qualified | It is qualified |
| Comparative example 1 | It is qualified | It is unqualified | It is unqualified |
As seen from Table 3, the compound essential oil stability obtained by the embodiment of the present invention is more preferably.
The above described is only a preferred embodiment of the present invention, limitation in any form not is done to the present invention, therefore
All contents without departing from technical solution of the present invention, it is made to the above embodiment according to the technical essence of the invention any simply to repair
Change, equivalent variations and modification, in the range of still falling within technical solution of the present invention.
Claims (10)
1. a kind of preparation method of compound essential oil, which is characterized in that include the following steps：
Step 1: rosemary leave, dried peppermint leaf are dried in the shade indoors；
Step 2: using rosemary leave, dried peppermint leaf and fennel fruit as raw material, raw material is subjected to infiltration and ultrasonication, then
Distillation essential oil is obtained using the method for distillation extraction again, wherein
The infiltration is specially：In rosemary leave, dried peppermint leaf and fennel fruit input distillation still that step 1 is obtained, addition is gone
Ionized water impregnates, wherein the deionized water weight is 5 ~ 10 times of the sum of rosemary leave, dried peppermint leaf and fennel fruit weight, leaching
Interruption is mixed slowly during bubble, 10 ~ 30 revs/min of mixing speed, until rosemary leave, dried peppermint leaf are infiltrated by deionized water
Fully；
Step 3: black pepper seed, sweet orange seed are mixed, crushed, CO is taken2The method of supercritical extract obtains extraction essential oil；
Step 4: the distillation essential oil that step 2 obtains and the extraction essential oil that step 3 obtains are mixed, it is still aging at room temperature,
Then the filter of 1 ~ 10 micron pore size is used to filter to get compound essential oil.
2. the preparation method of compound essential oil according to claim 1, which is characterized in that the rosemary leave, dried peppermint leaf, fennel
Fragrant fruit, black pepper seed, sweet orange seed weight ratio be：1：0.1~10：0.1~10：0.1~10：0.1~10.
3. the preparation method of compound essential oil according to claim 2, which is characterized in that the rosemary leave, dried peppermint leaf, fennel
Fragrant fruit, black pepper seed, sweet orange seed weight ratio be：1:5:2:3:2.
4. the preparation method of compound essential oil according to claim 1, which is characterized in that in the step 2, distillation extraction
Process conditions be：Vapo(u)rizing temperature is 100 ~ 120 DEG C.
5. the preparation method of compound essential oil according to claim 1, which is characterized in that in the step 2, the ultrasound
Wave is handled：Mixing speed is controlled at 10 ~ 50 revs/min, material in distillation still is heated to 80 ~ 100 DEG C, is adopted
With the ultrasonication 5 ~ 10 minutes that frequency is 30 ~ 100 KHZ.
6. the preparation method of compound essential oil according to claim 1, which is characterized in that in the step 3, CO2It is overcritical
The process conditions of extraction are：Pressure is 1 ~ 10MPa, 45 ~ 65 DEG C of extraction temperature, extraction time 60-80min in extraction kettle.
7. the preparation method of compound essential oil according to claim 1, which is characterized in that in the step 4, digestion time
It is 5 ~ 10 days.
8. a kind of compound essential oil, which is characterized in that using the preparation method such as claim 1-7 any one of them compound essential oils
The compound essential oil of preparation.
9. application of the compound essential oil according to any one of claims 8 as weight-reducing skin-care product additive, the weight-reducing skin care item are by compound
Essential oil and skin care field customary adjuvant are made.
10. a kind of weight-reducing massage oil, which is characterized in that include the component of following mass percentage：
Compound essential oil 0.1-10% according to any one of claims 8,
Grape（VITIS VINIFERA）Seed oil 0.1-10%,
Aloe（ALOE VERA）Extract 0.1-10%,
Sweet almond（PRUNUS AMYGDALUS DULCIS）Oily 0.1-10%,
Jojoba（SIMMONDSIA CHINENSIS）Seed oil 60-90%,
Tocopherol acetate 0.1-1%.
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| CN110037334A (en) * | 2019-05-10 | 2019-07-23 | 湖北中烟工业有限责任公司 | It is a kind of can lifting electrical heating do not burn the rich essence of cigarette flavor and its application |
| CN115531263A (en) * | 2022-09-06 | 2022-12-30 | 广州香乐谷生物科技有限公司 | Compound essential oil, application and preparation method thereof, and compound essential oil product |
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