CN110960464A - Anti-wrinkle eye cream containing plant source fermentation extract and preparation method thereof - Google Patents
Anti-wrinkle eye cream containing plant source fermentation extract and preparation method thereof Download PDFInfo
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Abstract
The invention provides an anti-wrinkle eye cream containing a plant source fermentation extract and a preparation method thereof, and relates to the technical field of cosmetics, wherein the anti-wrinkle eye cream is prepared from the plant source fermentation extract and an eye cream base material; the plant source fermentation extract is prepared by the following method: (1) drying herba Erigerontis, herba Polygoni Hydropiperis, and semen Celosiae, pulverizing, and sieving; (2) according to the mass ratio of 100: 10-40: 3-12 weighing fine powder of herba erigerontis, herba Polygoni Hydropiperis, and semen Celosiae, mixing well to obtain mixture, adding mixed deionized water, and sterilizing; cooling to room temperature, and adjusting the pH value to 6.5-7; inoculating zymocyte liquid, and performing fermentation culture after inoculation; (3) centrifuging the fermentation product, ultrasonically extracting the supernatant with organic solvent, collecting the organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermentation extract. The eye cream can effectively improve eye skin tissues, reduce wrinkles, promote blood circulation, relieve eye fatigue, relieve dark circles and improve the elasticity and smoothness of eye skin.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to anti-wrinkle eye cream containing a plant-derived fermented extract and a preparation method thereof.
Background
The eye skin is the thinnest skin of the human body and the part which moves most frequently, and is the place which pulls the skin the most frequently in the makeup, wrinkles are easy to grow out, and the wrinkles are difficult to disappear once growing out. Eye creams are essential for most people. In some special cases, eye creams should be used early, especially in women with dry skin, which is still high. It is understood that neutral skin is the majority at age 18-24, but neutral skin tends to assume a state of partial dryness or partial oiliness at some time. The second is a seasonal factor, many places are drier, especially the global winter warming phenomenon at present, so that the air humidity is smaller and smaller for a long time, and the long-time use of a heater and an air conditioner causes the face to lack water and the skin of eyes to rush to the beginning. Third, people who use eyes excessively, modern professional women mostly can not open characters and computers, and a large number of newspapers and video disks are added to dominate leisure time, and extreme fatigue of eye muscles causes skin wrinkles around eyes to appear too early.
The eye cream can protect the thin skin of eyes, has good moistening effect, and has certain effect on under-eye puffiness, black eye circles, crow's feet, dry lines, fine lines and the like. The eye cream needs to be mild because the skin of the eyes is very delicate and fragile and is easily damaged. No irritation and low sensitivity.
Chinese patent publication No. CN 103860426A discloses an eye cream, which organically combines raw materials such as safflower or saffron, tea, mint, organic silicon, etc. and cooperates with each other to act synergistically, and has the effects of moisturizing and nourishing, tightening skin, reducing wrinkles, relieving and eliminating eye fatigue, and preventing and eliminating black eye circles and under-eye puffiness.
Chinese patent with publication number CN 105943427A discloses a multi-effect moisturizing eye cream containing peony seed oil and a preparation method thereof. The eye cream is prepared from the following components: peony seed oil, sweet almond oil, buckwheat extract, emulsifier, witch hazel distillate, grapefruit extract, apple stem cells, vegetable glycerin, low-molecular hyaluronic acid, tea polyphenol, antibacterial agent and deionized water. The eye cream disclosed by the invention can accelerate blood microcirculation of skin around eyes, increase the activity of epidermal cells and enhance the elasticity of skin; can effectively remove free radicals, thereby inhibiting the formation of melanin and delaying the aging process of skin.
The eye cream uses plant extracts, but the price cost of the plants used by the plant extracts, such as safflower or saffron, peony seeds, witch hazel and the like, is higher, so the production cost of the eye cream can be directly increased. In nature, the application efficacy of more plants is not fully developed, so the plant source extract with low development cost and good eye care effect has better economic significance for the development of eye cream.
Erigeron breviscapus, also known as galum tenuifolium, galaxanthus canadensis, galaxanthus brevicaulis, and alpine yarrow herb. Is whole herb or fresh leaf of herba Otteliae Alismoidis of Compositae, and is perennial or annual herb. Most areas in China are distributed. Erigeron breviscapus mainly grows in open fields, wastelands, fields, valleys, ditches and roadside. Erigeron breviscapus can produce a large amount of lean fruits which fly away with the wind by virtue of the corolla and spread very quickly, and the roots and stems of the weeds are very thick and hard to remove. Therefore, farmers often cut off the erigeron breviscapus and take the erigeron breviscapus back as the pig grass to feed pigs. The erigeron breviscapus has the effects of clearing heat, promoting diuresis, removing blood stasis and reducing swelling. However, the use thereof in the cosmetic field has not been correspondingly developed and researched.
Disclosure of Invention
The invention aims to provide anti-wrinkle eye cream containing plant-derived fermentation extracts and a preparation method thereof, which can effectively improve eye skin tissues, reduce wrinkles, promote blood circulation, relieve eye fatigue, relieve dark circles and improve the elasticity and smoothness of eye skin.
In order to achieve the purpose, the invention is realized by the following technical scheme:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material;
the plant source fermentation extract is prepared by the following method:
(1) respectively drying herba erigerontis, herba Polygoni Hydropiperis and semen Celosiae until the water content is less than or equal to 8%, pulverizing, and sieving with 200 mesh sieve;
(2) according to the mass ratio of 100: 10-40: 3-12 weighing fine powder of herba erigerontis, herba Polygoni Hydropiperis, and semen Celosiae, mixing well to obtain mixture, adding deionized water 10-12 times of the mixture, sterilizing at 105 deg.C for 20-25 min; then cooling to room temperature, and adjusting the pH value to 6.5-7; inoculating zymocyte liquid, and performing fermentation culture after inoculation;
(3) centrifuging the obtained fermentation product, collecting supernatant, ultrasonically extracting the supernatant with organic solvent for 2-3 times, collecting organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermented extract.
Preferably, the zymocyte liquid contains lactobacillus bulgaricus and saccharomyces cerevisiae.
Preferably, the lactobacillus bulgaricus in the zymocyte liquid reaches 1-10 multiplied by 108cfu/L, the thallus in Saccharomyces cerevisiae reaches 5-15 multiplied by 109cfu/L。
Preferably, the organic solvent is one or a combination of ethyl acetate, dichloromethane, dichloroethane and chloroform.
Preferably, the mass of the organic solvent is 0.7-1.2 times of that of the supernatant liquid at each ultrasonic extraction; the technological parameters of ultrasonic extraction are as follows: the ultrasonic frequency is 30-50kHz, the power is 200-300W, and each time is 15-25 min.
Preferably, the mass ratio of the erigeron breviscapus, the polygonum hydropiper and the feather cockscomb seed is 100: 25: 8.
preferably, the specific steps of the fermentation culture are as follows: fermenting and culturing at 25 + -2 deg.C for 1 day, and culturing at 40 + -2 deg.C for 3-4 days; finally fermenting and culturing for 1 day at 50 +/-2 ℃.
Preferably, the eye cream base comprises the following raw materials: cetearyl alcohol, methyl glucoside sesquistearate, sodium hyaluronate, carbomer, 1, 3-butanediol, preservative, polyethylene glycol, caprylic/capric triglyceride, glycerol, plant essential oil and deionized water;
the preservative is methyl paraben or propyl paraben; the plant essential oil is one or more of rose essential oil, lavender essential oil, jasmine essential oil, sweet osmanthus essential oil and lily essential oil.
Preferably, the eye cream is prepared from the following raw materials in percentage by weight: 2-4.5% of plant source fermentation extract, 1-3% of cetearyl alcohol, 0.8-1.8% of methyl glucoside sesquistearate, 0.05-0.1% of sodium hyaluronate, 0.2-0.5% of carbomer, 6-10% of 1, 3-butanediol, 0.005-0.015% of preservative, 5001-2.5% of polyethylene glycol, 2-5% of caprylic/capric triglyceride, 5-8% of glycerol, 0.002-0.007% of plant essential oil and the balance of deionized water.
A method for preparing anti-wrinkle eye cream containing plant source fermentation extract comprises the following steps:
(1) placing cetostearyl alcohol, methyl glucoside sesquistearate, antiseptic, polyethylene glycol 500, caprylic/capric triglyceride, and plant essential oil in a stirring tank A, heating to 75-80 deg.C under stirring, and stirring at constant temperature for 10-15min to obtain component A;
(2) placing sodium hyaluronate, carbomer, 1, 3-butanediol, glycerol and deionized water in a stirring tank B, heating to 75-80 deg.C while stirring, and stirring at constant temperature for 10-15min to obtain component B;
(3) placing the component B in an emulsifying device, slowly adding the component A at 75-80 deg.C, and homogenizing for 20-25 min;
(4) cooling the emulsifying device to 50-55 deg.C, adding plant fermented extract, homogenizing for 15-20min, and cooling to room temperature to obtain the final product.
The invention has the beneficial effects that:
the erigeron breviscapus is used as a main raw material in the plant source fermentation extract, so that waste can be changed into valuable, and the production cost is effectively reduced. The polygonum hydropiper and the feather cockscomb seeds matched with the erigeron breviscapus are common plants, and the cost is low. Thereby effectively controlling the overall cost of the plant-derived fermented extract as a whole.
In the invention, the erigeron breviscapus, the polygonum hydropiper and the feather cockscomb seeds are fermented and extracted by an organic solvent, and the erigeron breviscapus fermented extract contains a large amount of oleanolic acid, so that the erigeron breviscapus fermented extract can effectively reduce skin wrinkles, has a good moistening effect and has a good effect of increasing skin elasticity. The polygonum hydropiper fermented extract contains more flavonoid, quinone and other substances, and has good antioxidant and antibacterial effects. On the basis, the plant-derived fermentation extract has a good antioxidant effect by matching with a proper amount of the feather cockscomb seed extract, can effectively improve eye skin tissues, reduce wrinkles, promote blood circulation, relieve eye fatigue, relieve dark circles and improve the elasticity and smoothness of eye skin.
The plant source fermentation extract obtained in the invention can be matched with any common basic substance of eye cream to have better protection effect. In order to obtain a better protection effect, the plant source fermentation extract is matched with cetearyl alcohol, methyl glucoside sesquistearate and sodium hyaluronate to prepare the eye cream with excellent comprehensive performance.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material.
The plant source fermentation extract is prepared by the following method:
(1) respectively drying herba Erigerontis, herba Polygoni Hydropiperis, and semen Celosiae until the water content is less than or equal to 8%, pulverizing, and sieving with 200 mesh sieve.
(2) According to the mass ratio of 100: 25: 8 weighing fine powder of erigeron breviscapus, polygonum hydropiper and feather cockscomb seeds, uniformly mixing to obtain a mixture, adding deionized water with the mass 10 times of that of the mixture, and sterilizing at 105 ℃ for 20 min; then cooling to room temperature, and adjusting the pH value to 7; inoculating zymocyte liquid, and performing fermentation culture after inoculation, wherein the fermentation culture comprises the following specific steps: fermenting and culturing at 25 + -2 deg.C for 1 day, and culturing at 40 + -2 deg.C for 4 days; finally fermenting and culturing for 1 day at 50 +/-2 ℃.
Fermentation bacteriaThe liquid contains Bulgaria lactobacillus and Saccharomyces cerevisiae. The preparation method of the zymocyte liquid comprises the following steps: inoculating the purified Lactobacillus acidophilus and Saccharomyces cerevisiae to YPD medium (pH 7.0), and culturing at 30 deg.C with shaking table to obtain zymocyte liquid. The content of Lactobacillus bulgaricus in zymocyte liquid is 8 × 108cfu/L, 10 multiplied by 10 thalli in saccharomyces cerevisiae9cfu/L。
(3) Centrifuging the obtained fermentation product, taking supernatant, and ultrasonically extracting the supernatant for 3 times by adopting dichloroethane with the mass 1 time of the mass, wherein the ultrasonic frequency is 50kHz, the power is 300W, and each time is 20 in; collecting organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermented extract.
The anti-wrinkle eye cream comprises the following specific formula:
3.5% of plant-derived fermented extract, 2% of cetearyl alcohol, 1.5% of methyl glucoside sesquistearate, 0.08% of sodium hyaluronate, 0.4% of carbomer, 8% of 1, 3-butanediol, 0.01% of methyl paraben, 5002% of polyethylene glycol, 3.5% of caprylic/capric triglyceride, 7% of glycerol, 0.005% of lavender essential oil and the balance of deionized water.
The preparation method of the anti-wrinkle eye cream comprises the following steps:
(1) placing cetearyl alcohol, methyl glucoside sesquistearate, methyl paraben, polyethylene glycol 500, caprylic/capric triglyceride, and lavender essential oil in a stirring tank A, heating to 80 deg.C under stirring, and stirring at constant temperature for 12min to obtain component A;
(2) placing sodium hyaluronate, carbomer, 1, 3-butanediol, glycerol and deionized water in a stirring tank B, heating to 80 deg.C while stirring, and stirring at constant temperature for 12min to obtain component B;
(3) placing the component B in an emulsifying device, slowly adding the component A at 75-80 deg.C, and homogenizing for 25 min;
(4) cooling the emulsifying device to 55 deg.C, adding the plant source fermented extract, homogenizing for 20min, and cooling to room temperature to obtain the final product.
Example 2:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material.
The plant source fermentation extract is prepared by the following method:
(1) respectively drying herba Erigerontis, herba Polygoni Hydropiperis, and semen Celosiae until the water content is less than or equal to 8%, pulverizing, and sieving with 200 mesh sieve.
(2) According to the mass ratio of 100: 30: 5 weighing fine powder of erigeron breviscapus, polygonum hydropiper and feather cockscomb seeds, uniformly mixing to obtain a mixture, adding deionized water with the mass being 12 times that of the mixture, and sterilizing at 105 ℃ for 22 min; then cooling to room temperature, and adjusting the pH value to 6.5; inoculating zymocyte liquid, and performing fermentation culture after inoculation, wherein the fermentation culture comprises the following specific steps: fermenting and culturing at 25 + -2 deg.C for 1 day, and culturing at 40 + -2 deg.C for-4 days; finally fermenting and culturing for 1 day at 50 +/-2 ℃.
The zymocyte liquid contains lactobacillus bulgaricus and saccharomyces cerevisiae. The preparation method of the zymocyte liquid comprises the following steps: inoculating the purified Lactobacillus acidophilus and Saccharomyces cerevisiae to YPD medium (pH 7.0), and culturing at 30 deg.C with shaking table to obtain zymocyte liquid. The content of Lactobacillus bulgaricus in zymocyte liquid is 8 × 108cfu/L, the thallus in Saccharomyces cerevisiae reaches 12 multiplied by 109cfu/L。
(3) Centrifuging the obtained fermentation product, taking supernatant, and ultrasonically extracting the supernatant for 3 times by using dichloromethane with the mass of 1.2 times of that of the supernatant, wherein the ultrasonic frequency is 35kHz, the power is 200W, and each time lasts for 25 min; collecting organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermented extract.
The anti-wrinkle eye cream comprises the following specific formula:
2.8% of plant-derived fermented extract, 1.8% of cetearyl alcohol, 1.5% of methyl glucoside sesquistearate, 0.06% of sodium hyaluronate, 0.2% of carbomer, 7% of 1, 3-butanediol, 0.01% of propyl hydroxybenzoate, 5001.5% of polyethylene glycol, 4.5% of caprylic/capric triglyceride, 7% of glycerol, 0.006% of rose essential oil and the balance of deionized water.
The preparation method of the anti-wrinkle eye cream comprises the following steps:
(1) placing cetearyl alcohol, methyl glucoside sesquistearate, propyl p-hydroxybenzoate, polyethylene glycol 500, caprylic/capric triglyceride, and oleum Rosae Rugosae in a stirring tank A, heating to 75-80 deg.C under stirring, and stirring at constant temperature for 10-15min to obtain component A;
(2) placing sodium hyaluronate, carbomer, 1, 3-butanediol, glycerol and deionized water in a stirring tank B, heating to 75-80 deg.C while stirring, and stirring at constant temperature for 10-15min to obtain component B;
(3) placing the component B in an emulsifying device, slowly adding the component A at 78 deg.C, and homogenizing for 25 min;
(4) cooling the emulsifying device to 52 deg.C, adding the plant source fermented extract, homogenizing for 16min, and cooling to room temperature to obtain the final product.
Example 3:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material.
The plant source fermentation extract is prepared by the following method:
(1) respectively drying herba Erigerontis, herba Polygoni Hydropiperis, and semen Celosiae until the water content is less than or equal to 8%, pulverizing, and sieving with 200 mesh sieve.
(2) According to the mass ratio of 100: 40: 3 weighing fine powder of erigeron breviscapus, polygonum hydropiper and feather cockscomb seeds, uniformly mixing to obtain a mixture, adding deionized water with the mass being 12 times that of the mixture, and sterilizing at 105 ℃ for 20 min; then cooling to room temperature, and adjusting the pH value to 7; inoculating zymocyte liquid, and performing fermentation culture after inoculation, wherein the fermentation culture comprises the following specific steps: fermenting and culturing at 25 + -2 deg.C for 1 day, and culturing at 40 + -2 deg.C for 4 days; finally fermenting and culturing for 1 day at 50 +/-2 ℃.
The zymocyte liquid contains lactobacillus bulgaricus and saccharomyces cerevisiae. The preparation method of the zymocyte liquid comprises the following steps: inoculating the purified Lactobacillus acidophilus and Saccharomyces cerevisiae to YPD medium (pH 7.0), and culturing at 30 deg.C with shaking table to obtain zymocyte liquid. The content of Lactobacillus gasseri in the zymocyte liquid reaches 10 × 108cfu/L, 10 multiplied by 10 thalli in saccharomyces cerevisiae9cfu/L。
(3) Centrifuging the obtained fermentation product, taking supernatant, and ultrasonically extracting the supernatant for 3 times by using ethyl acetate with the mass of 0.7 time of that of the supernatant, wherein the ultrasonic frequency is 50kHz, the power is 300W, and each time is 20 min; collecting organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermented extract.
The anti-wrinkle eye cream comprises the following specific formula:
4.5% of plant-derived fermented extract, 2% of cetearyl alcohol, 1.8% of methyl glucoside sesquistearate, 0.05% of sodium hyaluronate, 0.3% of carbomer, 9% of 1, 3-butanediol, 0.01% of methyl paraben, 5002.5% of polyethylene glycol, 2% of caprylic/capric triglyceride, 5% of glycerol, 0.005% of jasmine essential oil and the balance of deionized water.
The preparation method of the anti-wrinkle eye cream comprises the following steps:
(1) placing cetearyl alcohol, methyl glucoside sesquistearate, methyl paraben, polyethylene glycol 500, caprylic/capric triglyceride, and flos Jasmini sambac essential oil in a stirring tank A, heating to 75-80 deg.C under stirring, and stirring at constant temperature for 10-15min to obtain component A;
(2) placing sodium hyaluronate, carbomer, 1, 3-butanediol, glycerol and deionized water in a stirring tank B, heating to 80 deg.C while stirring, and stirring at constant temperature for 10min to obtain component B;
(3) placing the component B in an emulsifying device, slowly adding the component A at 80 deg.C, and homogenizing for 20-25 min;
(4) cooling the emulsifying device to 55 deg.C, adding the plant source fermented extract, homogenizing for 15min, and cooling to room temperature to obtain the final product.
Example 4:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material.
The plant source fermentation extract is prepared by the following method:
(1) respectively drying herba Erigerontis, herba Polygoni Hydropiperis, and semen Celosiae until the water content is less than or equal to 8%, pulverizing, and sieving with 200 mesh sieve.
(2) According to the mass ratio of 100: 25: 12 weighing fine powder of erigeron breviscapus, polygonum hydropiper and feather cockscomb seeds, uniformly mixing to obtain a mixture, adding deionized water with the mass of 10 times of that of the mixture, and sterilizing at 105 ℃ for 25 min; then cooling to room temperature, and adjusting the pH value to 7; inoculating zymocyte liquid, and performing fermentation culture after inoculation, wherein the fermentation culture comprises the following specific steps: fermenting and culturing at 25 + -2 deg.C for 1 day, and culturing at 40 + -2 deg.C for 4 days; finally fermenting and culturing for 1 day at 50 +/-2 ℃.
The zymocyte liquid contains lactobacillus bulgaricus and saccharomyces cerevisiae. The preparation method of the zymocyte liquid comprises the following steps: inoculating the purified Lactobacillus acidophilus and Saccharomyces cerevisiae to YPD medium (pH 7.0), and culturing at 30 deg.C with shaking table to obtain zymocyte liquid. The lactobacillus bulgaricus in the zymocyte liquid reaches 1 × 108cfu/L, the thallus in Saccharomyces cerevisiae reaches 15 multiplied by 109cfu/L。
(3) Centrifuging the obtained fermentation product, taking supernatant, and ultrasonically extracting the supernatant with 1 time of chloroform by the ultrasonic frequency of 30kHz and power of 280W for 25min each time for 2 times; collecting organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermented extract.
The anti-wrinkle eye cream comprises the following specific formula:
2% of plant-derived fermentation extract, 3% of cetearyl alcohol, 0.8% of methyl glucoside sesquistearate, 0.08% of sodium hyaluronate, 0.2% of carbomer, 10% of 1, 3-butanediol, 0.005% of methyl paraben, 5001% of polyethylene glycol, 5% of caprylic/capric triglyceride, 8% of glycerol, 0.002% of lily essential oil and the balance of deionized water.
(1) Placing cetearyl alcohol, methyl glucoside sesquistearate, methyl paraben, polyethylene glycol 500, caprylic/capric triglyceride, and flos Lilii essential oil in a stirring tank A, heating to 75-80 deg.C under stirring, and stirring at constant temperature for 10-15min to obtain component A;
(2) placing sodium hyaluronate, carbomer, 1, 3-butanediol, glycerol and deionized water in a stirring tank B, heating to 75 deg.C while stirring, and stirring at constant temperature for 15min to obtain component B;
(3) placing the component B in an emulsifying device, slowly adding the component A at 75 deg.C, and homogenizing for 20-25 min;
(4) cooling the emulsifying device to 53 deg.C, adding the plant source fermented extract, homogenizing for 20min, and cooling to room temperature to obtain the final product.
Example 5:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material.
The plant source fermentation extract is prepared by the following method:
(1) respectively drying herba Erigerontis, herba Polygoni Hydropiperis, and semen Celosiae until the water content is less than or equal to 8%, pulverizing, and sieving with 200 mesh sieve.
(2) According to the mass ratio of 100: 10: 8 weighing fine powder of erigeron breviscapus, polygonum hydropiper and feather cockscomb seeds, uniformly mixing to obtain a mixture, adding deionized water with the mass being 11 times that of the mixture, and sterilizing at 105 ℃ for 25 min; then cooling to room temperature, and adjusting the pH value to 6.5; inoculating zymocyte liquid, and performing fermentation culture after inoculation, wherein the fermentation culture comprises the following specific steps: fermenting and culturing at 25 + -2 deg.C for 1 day, and culturing at 40 + -2 deg.C for 3 days; finally fermenting and culturing for 1 day at 50 +/-2 ℃.
The zymocyte liquid contains lactobacillus bulgaricus and saccharomyces cerevisiae. The preparation method of the zymocyte liquid comprises the following steps: inoculating the purified Lactobacillus acidophilus and Saccharomyces cerevisiae to YPD medium (pH 7.0), and culturing at 30 deg.C with shaking table to obtain zymocyte liquid. The Lactobacillus bulgaricus in the zymocyte liquid reaches 5 × 108cfu/L, 5X 10 thalli in saccharomyces cerevisiae9cfu/L。
(3) Centrifuging the obtained fermentation product, collecting supernatant, and ultrasonically extracting the supernatant with 1.2 times of chloroform for 2 times at ultrasonic frequency of 40kHz and power of 200W for 15min each time; collecting organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermented extract.
The anti-wrinkle eye cream comprises the following specific formula:
3% of plant source fermentation extract, 1% of cetearyl alcohol, 1.3% of methyl glucoside sesqui-propylparaben, 0.1% of sodium hyaluronate, 0.5% of carbomer, 6% of 1, 3-butanediol, 0.015% of preservative, 5001.8% of polyethylene glycol, 35% of caprylic/capric triglyceride, 7% of glycerol, 0.007% of sweet osmanthus essential oil and the balance of deionized water.
(1) Placing cetostearyl alcohol, methyl glucoside sesquistearate, propyl p-hydroxybenzoate, polyethylene glycol 500, caprylic/capric triglyceride, and flos Osmanthi Fragrantis essential oil in a stirring tank A, heating to 75-80 deg.C under stirring, and stirring at constant temperature for 10-15min to obtain component A;
(2) placing sodium hyaluronate, carbomer, 1, 3-butanediol, glycerol and deionized water in a stirring tank B, heating to 78 deg.C while stirring, and stirring at constant temperature for 12min to obtain component B;
(3) placing the component B in an emulsifying device, slowly adding the component A at 78 deg.C, and homogenizing for 20-25 min;
(4) cooling the emulsifying device to 50 deg.C, adding the plant source fermented extract, homogenizing for 18min, and cooling to room temperature to obtain the final product.
Comparative example 1:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material; in contrast to example 1, in the preparation of the plant-derived fermented extract, the plant-derived material was erigeron breviscapus. The rest of the process was the same as in example 1.
Comparative example 2:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material; different from the embodiment 1, when the plant source fermentation extract is prepared, the plant source raw materials are erigeron breviscapus and polygonum hydropiper, and the mass ratio of the erigeron breviscapus to the polygonum hydropiper is 100: 10. the rest of the process was the same as in example 1.
Comparative example 3:
an anti-wrinkle eye cream containing plant source fermented extract is prepared from plant source fermented extract and eye cream base material; different from the embodiment 1, when the plant source fermentation extract is prepared, the plant source raw materials are erigeron breviscapus and feather cockscomb seeds, and the mass ratio is 100: 8. the rest of the process was the same as in example 1.
And (3) testing:
1. general data:
160 healthy female subjects with age of 30-42 years and mild to moderate eye wrinkles were selected.
Subject exclusion criteria: those with allergic constitution; pregnant, lactating; patients with acute inflammation or other cutaneous disease of the face; other dermatologists who have participated in clinical studies or structures on the face currently or in approximately three months; those who currently use any anti-inflammatory drug or immunosuppressant within approximately 1 month or more.
Subject termination and withdrawal criteria: the subject produces adverse reactions or special physiological and pathological changes; use of non-approved drugs; too much UV radiation.
2. Test method
160 healthy female subjects were randomly divided into 8 test groups, test groups 1 to 5 tested the anti-wrinkle eye cream of examples 1 to 5; test groups 6-8 test the anti-wrinkle eye creams in comparative examples 1-3. The test subjects take a proper amount of corresponding eye cream to be smeared around the eyes in the morning and evening, and the test time is 10.12-11.23 days.
Measuring by an instrument:
the elasticity of the skin of the eyes of the subject was evaluated using a skin elasticity tester cutometer and analyzed using a facial image analyzer VISIA-CR. Skin improvement was assessed and analyzed every three weeks and recorded 3 weeks and 6 weeks after eye cream application. Specific results are shown in table 1.
Table 1:
as can be seen from table 1, the eye cream of the present invention has a good effect of improving the eye skin, can reduce wrinkles, and make the skin smooth and elastic, and also has a good effect of improving the uniformity of the eye skin. In the comparative example, the effect is reduced to some extent, and a certain synergistic effect is observed among the three.
Subjective evaluation of subjects:
after 6 weeks using the eye cream, the subjects completed the self-evaluation questionnaire, and completed the efficacy evaluation of the anti-wrinkle eye cream in examples 1 to 3, and the specific evaluation results are shown in tables 2 to 4.
Table 2: anti-wrinkle eye cream of example 1
| Is very good | Good taste | In general | Not good | |
| Moisture retention | 13 | 6 | 1 | 0 |
| Degree of elasticity | 10 | 8 | 2 | 0 |
| Degree of wrinkle improvement | 14 | 5 | 1 | 0 |
| Moistening degree | 9 | 9 | 2 | 0 |
| Smell(s) | 10 | 10 | 0 | 0 |
Table 3: anti-wrinkle eye cream according to example 2
| Is very good | Good taste | In general | Not good | |
| Moisture retention | 12 | 7 | 1 | 0 |
| Degree of elasticity | 11 | 6 | 3 | 0 |
| Degree of wrinkle improvement | 12 | 6 | 2 | 0 |
| Moistening degree | 10 | 7 | 3 | 0 |
| Smell(s) | 13 | 6 | 1 | 0 |
Table 4: anti-wrinkle eye cream according to example 3
| Is very good | Good taste | In general | Not good | |
| Moisture retention | 10 | 10 | 0 | 0 |
| Degree of elasticity | 9 | 9 | 2 | 0 |
| Degree of wrinkle improvement | 13 | 6 | 1 | 0 |
| Moistening degree | 10 | 10 | 0 | 0 |
| Smell(s) | 9 | 10 | 1 | 0 |
As can be seen from tables 2-4, the subjects have high satisfaction degree on the anti-wrinkle eye cream provided by the invention, and the anti-wrinkle eye cream has good market prospect.
And (3) safety evaluation:
no adverse events related to the test occurred during the whole test. The eye cream has better safety.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. An anti-wrinkle eye cream containing a plant-derived fermented extract is characterized by being prepared from the plant-derived fermented extract and an eye cream base material;
the plant source fermentation extract is prepared by the following method:
(1) respectively drying herba erigerontis, herba Polygoni Hydropiperis and semen Celosiae until the water content is less than or equal to 8%, pulverizing, and sieving with 200 mesh sieve;
(2) according to the mass ratio of 100: 10-40: 3-12 weighing fine powder of herba erigerontis, herba Polygoni Hydropiperis, and semen Celosiae, mixing well to obtain mixture, adding deionized water 10-12 times of the mixture, sterilizing at 105 deg.C for 20-25 min; then cooling to room temperature, and adjusting the pH value to 6.5-7; inoculating zymocyte liquid, and performing fermentation culture after inoculation;
(3) centrifuging the obtained fermentation product, collecting supernatant, ultrasonically extracting the supernatant with organic solvent for 2-3 times, collecting organic layer, filtering, and evaporating under reduced pressure to obtain plant-derived fermented extract.
2. The anti-wrinkle eye cream containing the plant-derived fermented extract according to claim 1, wherein the fermentation broth contains lactobacillus bulgaricus and saccharomyces cerevisiae.
3. The anti-wrinkle eye cream containing the plant-derived fermented extract according to claim 2, wherein the Lactobacillus bulgaricus is contained in the fermentation broth to 1-10 x 108cfu/L, the thallus in Saccharomyces cerevisiae reaches 5-15 multiplied by 109cfu/L。
4. The anti-wrinkle eye cream containing plant-derived fermented extracts according to claim 1, wherein the organic solvent is one or a combination of ethyl acetate, dichloromethane, dichloroethane and chloroform.
5. The anti-wrinkle eye cream containing the plant-derived fermented extract as claimed in claim 1, wherein the mass of the organic solvent is 0.7-1.2 times of the mass of the supernatant liquid at each ultrasonic extraction; the technological parameters of ultrasonic extraction are as follows: the ultrasonic frequency is 30-50kHz, the power is 200-300W, and each time is 15-25 min.
6. The anti-wrinkle eye cream containing the plant-derived fermented extract as claimed in claim 1, wherein the mass ratio of the erigeron breviscapus, the polygonum hydropiper and the feather cockscomb seeds is 100: 25: 8.
7. the anti-wrinkle eye cream containing plant-derived fermented extracts according to claim 1, wherein the fermentation culture comprises the following specific steps: fermenting and culturing at 25 + -2 deg.C for 1 day, and culturing at 40 + -2 deg.C for 3-4 days; finally fermenting and culturing for 1 day at 50 +/-2 ℃.
8. The anti-wrinkle eye cream containing the plant-derived fermented extract as claimed in claim 1, wherein the eye cream base material comprises the following raw materials: cetearyl alcohol, methyl glucoside sesquistearate, sodium hyaluronate, carbomer, 1, 3-butanediol, preservative, polyethylene glycol, caprylic/capric triglyceride, glycerol, plant essential oil and deionized water;
the preservative is methyl paraben or propyl paraben; the plant essential oil is one or more of rose essential oil, lavender essential oil, jasmine essential oil, sweet osmanthus essential oil and lily essential oil.
9. The anti-wrinkle eye cream containing plant-derived fermented extracts as claimed in claim 8, wherein the eye cream is prepared from the following raw materials in percentage by weight: 2-4.5% of plant source fermentation extract, 1-3% of cetearyl alcohol, 0.8-1.8% of methyl glucoside sesquistearate, 0.05-0.1% of sodium hyaluronate, 0.2-0.5% of carbomer, 6-10% of 1, 3-butanediol, 0.005-0.015% of preservative, 5001-2.5% of polyethylene glycol, 2-5% of caprylic/capric triglyceride, 5-8% of glycerol, 0.002-0.007% of plant essential oil and the balance of deionized water.
10. The method for preparing anti-wrinkle eye cream containing plant-derived fermented extract according to claim 8 or 9, comprising the steps of:
(1) placing cetostearyl alcohol, methyl glucoside sesquistearate, antiseptic, polyethylene glycol 500, caprylic/capric triglyceride, and plant essential oil in a stirring tank A, heating to 75-80 deg.C under stirring, and stirring at constant temperature for 10-15min to obtain component A;
(2) placing sodium hyaluronate, carbomer, 1, 3-butanediol, glycerol and deionized water in a stirring tank B, heating to 75-80 deg.C while stirring, and stirring at constant temperature for 10-15min to obtain component B;
(3) placing the component B in an emulsifying device, slowly adding the component A at 75-80 deg.C, and homogenizing for 20-25 min;
(4) cooling the emulsifying device to 50-55 deg.C, adding plant fermented extract, homogenizing for 15-20min, and cooling to room temperature to obtain the final product.
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