CN108739178B - Sowing and seedling raising method for increasing content of syringin and alkynin of codonopsis pilosula - Google Patents
Sowing and seedling raising method for increasing content of syringin and alkynin of codonopsis pilosula Download PDFInfo
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Abstract
The invention belongs to the technical field of traditional Chinese medicine planting, and particularly relates to a sowing and seedling raising method for improving the content of syringin and lobetyolin of codonopsis pilosula, which comprises the following steps: (1) magnetizing the fully-granulated codonopsis pilosula seeds, immersing the codonopsis pilosula seeds in a cobalt oxalate solution for soaking and continuously stirring, taking out the filtrate, and draining until no water drops drop to obtain the processed codonopsis pilosula seeds; (2) sowing the treated codonopsis pilosula seeds in seedling raising holes of a seedling raising substrate, sowing one seed in each hole, wherein the sowing depth is 1.2-1.4 cm, and transplanting after seedling emergence for 40-45 days; the seedling culture substrate is prepared from the following raw materials: grape skin residue, tomato seed meal, purple sweet potato vine, acacia flower, pond sludge, activated medical stone powder, andrographolide, chlorogenic acid and chufa polysaccharide; the emergence rate of the codonopsis pilosula is improved, the cold resistance, drought resistance and stress resistance of the codonopsis pilosula are enhanced, the transformation, synthesis and accumulation of syringin and lobetyolin are promoted, the medicinal health-care value of the codonopsis pilosula is effectively improved, and the economic benefit of codonopsis pilosula farmers is increased.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine planting, and particularly relates to a sowing and seedling raising method for improving the content of syringin and lobetyolin in codonopsis pilosula.
Background
Radix Codonopsis is dried root of radix Codonopsis, radix Codonopsis Lanceolatae or radix Codonopsis Lanceolatae of Campanulaceae, has sweet taste and mild property, has effects of invigorating spleen and replenishing qi, invigorating spleen and benefiting lung, and removing polydipsia, and can be used for treating spleen and lung weakness, short breath and cardiopalmus, anorexia and loose stool, internal heat and diabetes, etc., and radix Codonopsis is often used for stewing chicken, stewing lean meat, etc. due to its effects of invigorating qi and invigorating spleen; the radix codonopsitis mainly contains active ingredients such as lobetyolin, syringin and the like; the dangshen alkynoside has a good protection effect on gastric mucosa injury caused by ethanol, is consistent with the traditional effect of dangshen for tonifying middle-jiao and Qi, and is an active ingredient with the protection effect on the gastric mucosa of the dangshen; syringin has pharmacological effects of relieving fatigue, relieving cerebral ischemia, and enhancing adaptability; therefore, the method for effectively improving the content of syringin and lobetyolin in codonopsis pilosula can effectively improve the health care value of codonopsis pilosula, and has great significance for the development of codonopsis pilosula industry.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a sowing and seedling raising method for improving the content of syringin and lobetyolin of codonopsis pilosula.
The invention is realized by the following technical scheme:
a sowing and seedling raising method for improving the content of syringin and alkynin of radix codonopsis comprises the following specific steps:
(1) magnetizing the fully-granulated codonopsis pilosula seeds for 710-790 s, immersing the codonopsis pilosula seeds in a cobalt oxalate solution for 32-36 min, continuously stirring, taking out filtrate, and draining until no water drops drop to obtain the processed codonopsis pilosula seeds;
(2) sowing the treated codonopsis pilosula seeds in seedling raising holes of a seedling raising substrate, sowing one seed in each hole, wherein the sowing depth is 1.2-1.4 cm, and transplanting after seedling emergence for 40-45 days;
the seedling raising substrate is prepared from the following raw materials in parts by weight: 34-38 parts of grape skin residues, 21-23 parts of tomato seed meal, 15-17 parts of purple sweet potato vine, 8-9 parts of acacia flower, 110-120 parts of pond sludge, 8-9 parts of activated medical stone powder, 0.12-0.14 part of andrographolide, 0.16-0.18 part of chlorogenic acid and 0.12-0.14 part of chufa polysaccharide.
As a further scheme of the invention: the magnetization treatment in the step (1) comprises the steps of magnetization treatment for 100-120 s under the condition that the magnetic field intensity is 1120-1140G, pause for 30-40 s, magnetization treatment for 190-210 s under the condition that the magnetic field intensity is 640-660G, pause for 50-60 s, and magnetization treatment for 340-360 s under the condition that the magnetic field intensity is 640-660G.
As a further scheme of the invention: the cobalt oxalate solution in the step (1) is prepared according to the following steps: 1.7-1.9 parts by weight of cobalt oxalate and 0.22-0.24 part by weight of mesona polysaccharide are put into 240-260 parts by weight of water and stirred until dissolved, so as to obtain a cobalt oxalate solution.
As a further scheme of the invention: the activated medical stone powder in the step (2) is prepared according to the following steps: crushing medical stone, putting the crushed medical stone into a rotary frying pan with the temperature of 102-108 ℃ for constant-temperature frying for 28-30 min, taking out, grinding the medical stone into powder, putting the powder into lithium hydroxide aqueous solution with the weight of 0.5-0.6 times of the powder and the mass concentration of 4.4-4.8%, uniformly stirring the solution, carrying out ultrasonic treatment for 14-16 min under the conditions of the power of 160-170W and the frequency of 96-100 kHz, and carrying out spray drying at the temperature of 71-75 ℃ to obtain the activated medical stone powder.
As a further scheme of the invention: the preparation method of the seedling substrate in the step (2) specifically comprises the following steps:
A. mixing and crushing grape skin residues, tomato seed meal, purple sweet potato vines and acacia flowers, performing constant-temperature steam treatment for 13-15 min at the temperature of 90-94 ℃, performing constant-temperature steam treatment for 21-25 min at the temperature of 141-149 ℃, putting into a fermentation tank at the temperature of 43-45 ℃ for constant-temperature fermentation for 140-160 h, and taking out to obtain fermentation pulp;
B. uniformly mixing and stirring the fermentation pulp and the pond sludge, covering a plastic film, fermenting at a constant temperature of 37-39 ℃ for 280-300 h, uniformly mixing and stirring with activated medical stone powder, andrographolide, chlorogenic acid and water chestnut polysaccharide, and adjusting the water content to 75-80% to obtain the seedling culture substrate.
The invention has the beneficial effects that: according to the sowing and seedling raising method for improving the content of syringin and lobetyolin in codonopsis pilosula provided by the invention, magnetization treatment is carried out before sowing and soaking in a cobaltous oxalate solution is combined, so that the activity of codonopsis pilosula seed enzymes is effectively improved, the vitality of codonopsis pilosula seeds is enhanced, the emergence rate of codonopsis pilosula is improved, the cold resistance, drought resistance and stress resistance of codonopsis pilosula is enhanced, the nutrient absorption, transformation and accumulation capacity of codonopsis pilosula is enhanced, and a good foundation is laid for the later-period root growth and; the seedling raising substrate disclosed by the invention is used for raising seedlings, has rich organic matters, can fully meet the nutritional requirements during the seedling raising period of the codonopsis pilosula, effectively promotes the rapid germination and seedling emergence of the codonopsis pilosula, promotes the division, differentiation and growth of roots of the codonopsis pilosula in seconds, enables the seedlings to be strong, enables the codonopsis pilosula seedlings to have higher biological activity and stronger environmental adaptability, can fully utilize nutrients after transplantation, promotes the expansion and growth of the roots, effectively promotes the transformation, synthesis and accumulation of syringin and tangshen alkynes, further effectively improves the medicinal health-care value of the codonopsis pilosula, and increases the economic benefits of codonopsis pilosula growers.
Detailed Description
The invention is illustrated by the following specific examples, which are not intended to be limiting.
Example 1
In the embodiment of the invention, a sowing and seedling raising method for improving the content of syringin and lobetyolin of codonopsis pilosula is as follows:
(1) magnetizing the fully-granulated codonopsis pilosula seeds for 710s, immersing the codonopsis pilosula seeds in a cobalt oxalate solution for 32min, continuously stirring, taking out the filtrate, and draining until no water drops drop to obtain the processed codonopsis pilosula seeds;
(2) sowing the treated codonopsis pilosula seeds in seedling raising holes of a seedling raising substrate, sowing one seed in each hole, wherein the sowing depth is 1.2-1.4 cm, and transplanting after seedling emergence for 40-45 days;
the seedling raising substrate is prepared from the following raw materials in parts by weight: 34 parts of grape skin residues, 21 parts of tomato seed meal, 15 parts of purple sweet potato vines, 8 parts of acacia flower, 110 parts of pond sludge, 8 parts of activated medical stone powder, 0.12 part of andrographolide, 0.16 part of chlorogenic acid and 0.12 part of chufa polysaccharide.
As a further scheme of the invention: the magnetization processing in the step (1) is performed for 100s under the condition that the magnetic field intensity is 1120G, and is suspended for 30s, is performed for 190s under the condition that the magnetic field intensity is 640G, is suspended for 50s, and is performed for 340s under the condition that the magnetic field intensity is 640G.
As a further scheme of the invention: the cobalt oxalate solution in the step (1) is prepared according to the following steps: 1.7 parts by weight of cobalt oxalate and 0.22 part by weight of mesona polysaccharide are put into 240 parts by weight of water and stirred until being dissolved, and then cobalt oxalate solution is obtained.
As a further scheme of the invention: the activated medical stone powder in the step (2) is prepared according to the following steps: pulverizing Maifanitum, parching in a rotary frying pan at 102 deg.C for 28min, taking out, grinding into powder, adding into 0.5 times of lithium hydroxide water solution with mass concentration of 4.4%, stirring, ultrasonic treating at power of 160W and frequency of 96kHz for 14min, and spray drying at 71 deg.C to obtain activated Maifanitum powder.
As a further scheme of the invention: the preparation method of the seedling substrate in the step (2) specifically comprises the following steps:
A. mixing and crushing grape skin residues, tomato seed meal, purple sweet potato vine and acacia flower, performing constant-temperature steam treatment for 13min at the temperature of 90 ℃, performing constant-temperature steam treatment for 21min at the temperature of 141 ℃, putting into a fermentation tank at the temperature of 43 ℃ for constant-temperature fermentation for 140h, and taking out to obtain fermentation pulp;
B. mixing and stirring the fermentation slurry and the pond sludge uniformly, covering a plastic film, fermenting at a constant temperature of 37 to 80 ℃ for 280 hours, mixing and stirring uniformly with activated medical stone powder, andrographolide, chlorogenic acid and chufa polysaccharide, and adjusting the water content to 75 to 80 percent to obtain the seedling culture substrate.
Example 2
In the embodiment of the invention, a sowing and seedling raising method for improving the content of syringin and lobetyolin of codonopsis pilosula is as follows:
(1) magnetizing the fully-granulated codonopsis pilosula seeds for 750s, immersing the codonopsis pilosula seeds in a cobalt oxalate solution for 34min, continuously stirring, taking out the filtrate, and draining until no water drops drop to obtain the processed codonopsis pilosula seeds;
(2) sowing the treated codonopsis pilosula seeds in seedling raising holes of a seedling raising substrate, sowing one seed in each hole, wherein the sowing depth is 1.2-1.4 cm, and transplanting after seedling emergence for 40-45 days;
the seedling raising substrate is prepared from the following raw materials in parts by weight: 36 parts of grape skin residues, 22 parts of tomato seed meal, 16 parts of purple sweet potato vine, 8.5 parts of acacia flower, 115 parts of pond sludge, 8.5 parts of activated medical stone powder, 0.13 part of andrographolide, 0.17 part of chlorogenic acid and 0.13 part of chufa polysaccharide.
As a further scheme of the invention: the magnetization processing in the step (1) is performed for 110s under the condition that the magnetic field intensity is 1130G, and is suspended for 35s, for 200s under the condition that the magnetic field intensity is 650G, and is suspended for 55s, and for 350s under the condition that the magnetic field intensity is 650G.
As a further scheme of the invention: the cobalt oxalate solution in the step (1) is prepared according to the following steps: 1.8 parts by weight of cobalt oxalate and 0.23 part by weight of mesona polysaccharide are put into 250 parts by weight of water and stirred until the cobalt oxalate solution is dissolved, thus obtaining the cobalt oxalate solution.
As a further scheme of the invention: the activated medical stone powder in the step (2) is prepared according to the following steps: pulverizing Maifanitum, parching at 105 deg.C for 29min, taking out, grinding into powder, adding into 0.55 times of lithium hydroxide aqueous solution with mass concentration of 4.6%, stirring, ultrasonic treating at power of 165W and frequency of 98kHz for 15min, and spray drying at 73 deg.C to obtain activated Maifanitum powder.
As a further scheme of the invention: the preparation method of the seedling substrate in the step (2) specifically comprises the following steps:
A. mixing and crushing grape skin residues, tomato seed meal, purple sweet potato vine and acacia flower, performing constant-temperature steam treatment for 14min at the temperature of 92 ℃, performing constant-temperature steam treatment for 23min at the temperature of 145 ℃, putting into a fermentation tank at the temperature of 44 ℃ for constant-temperature fermentation for 150h, and taking out to obtain fermentation pulp;
B. uniformly mixing and stirring the fermentation pulp and the pond sludge, covering a plastic film, fermenting for 290 hours at a constant temperature of 38 ℃, uniformly mixing and stirring the fermentation pulp and the pond sludge with activated medical stone powder, andrographolide, chlorogenic acid and chufa polysaccharide, and adjusting the water content to 75-80% to obtain the seedling culture substrate.
Example 3
In the embodiment of the invention, a sowing and seedling raising method for improving the content of syringin and lobetyolin of codonopsis pilosula is as follows:
(1) magnetizing 790s the plump codonopsis pilosula seeds, immersing the codonopsis pilosula seeds in a cobalt oxalate solution for 36min, continuously stirring, taking out the filtrate, and draining until no water drops drop to obtain the processed codonopsis pilosula seeds;
(2) sowing the treated codonopsis pilosula seeds in seedling raising holes of a seedling raising substrate, sowing one seed in each hole, wherein the sowing depth is 1.2-1.4 cm, and transplanting after seedling emergence for 40-45 days;
the seedling raising substrate is prepared from the following raw materials in parts by weight: 38 parts of grape skin residues, 23 parts of tomato seed meal, 17 parts of purple sweet potato vine, 9 parts of acacia flower, 120 parts of pond sludge, 9 parts of activated medical stone powder, 0.14 part of andrographolide, 0.18 part of chlorogenic acid and 0.14 part of chufa polysaccharide.
As a further scheme of the invention: and (2) performing the magnetization treatment in the step (1), wherein the magnetization treatment is performed for 120s under the condition that the magnetic field strength is 1140G, and is suspended for 40s, the magnetization treatment is performed for 210s under the condition that the magnetic field strength is 660G, and is suspended for 60s, and the magnetization treatment is performed for 360s under the condition that the magnetic field strength is 660G.
As a further scheme of the invention: the cobalt oxalate solution in the step (1) is prepared according to the following steps: 1.9 parts by weight of cobalt oxalate and 0.24 part by weight of mesona polysaccharide are put into 260 parts by weight of water and stirred until being dissolved, and then cobalt oxalate solution is obtained.
As a further scheme of the invention: the activated medical stone powder in the step (2) is prepared according to the following steps: crushing medical stone, frying in a rotary frying pan at 108 ℃ for 30min at constant temperature, taking out, grinding into powder, putting into 0.6 times of lithium hydroxide aqueous solution with mass concentration of 4.8%, uniformly stirring, carrying out ultrasonic treatment for 16min under the conditions of power of 160-170W and frequency of 100kHz, and carrying out spray drying at 75 ℃ to obtain the activated medical stone powder.
As a further scheme of the invention: the preparation method of the seedling substrate in the step (2) specifically comprises the following steps:
A. mixing and crushing grape skin residues, tomato seed meal, purple sweet potato vine and acacia flower, performing constant-temperature steam treatment for 15min at the temperature of 94 ℃, performing constant-temperature steam treatment for 25min at the temperature of 149 ℃, putting into a fermentation tank at the temperature of 45 ℃ for constant-temperature fermentation for 160h, and taking out to obtain fermentation slurry;
B. uniformly mixing and stirring the fermentation pulp and the pond sludge, covering a plastic film, fermenting for 300 hours at a constant temperature of 39 ℃, uniformly mixing and stirring the fermentation pulp and the pond sludge with activated medical stone powder, andrographolide, chlorogenic acid and water chestnut polysaccharide, and adjusting the water content to 75-80% to obtain the seedling culture substrate.
Comparative example 1
A sowing and seedling raising method for improving the content of syringin and alkynin of radix codonopsis is different from that in embodiment 1 in that magnetization treatment is not carried out in step (1) and other conditions are the same.
Comparative example 2
A sowing and seedling raising method for improving the content of syringin and alkynin of radix codonopsis is different from that in embodiment 1 in that the cobalt oxalate solution in the step (1) is replaced by water, and other conditions are the same.
Comparative example 3
A sowing and seedling raising method for improving the content of syringin and alkynin of radix codonopsis is different from that in example 1 in that acacia flowers, activated medical stone powder and andrographolide are not added into a seedling raising substrate in the step (2), and other conditions are the same.
Comparative example 4
A sowing and seedling raising method for improving the content of syringin and lobetyolin of codonopsis pilosula is different from that in the embodiment 1, purple sweet potato vine, chlorogenic acid and chufa polysaccharide are not added into a seedling raising substrate in the step (2), and other conditions are the same.
The same batch of codonopsis pilosula seeds were sown and cultured by using the methods of the examples and comparative examples, and were transplanted after the seedling culture under the same conditions except for different seedling culture methods, and were harvested two years after transplantation, and the content of syringin and the content of lobetyolin in codonopsis pilosula were measured after harvesting, and the results are shown in table 1:
TABLE 1 comparative results of examples and comparative examples
As can be seen from Table 1, the seedling raising method provided by the invention can significantly improve the syringin content and lobetyolin content of codonopsis pilosula, and greatly improve the medicinal health care value of codonopsis pilosula.
Claims (1)
1. A sowing and seedling raising method for improving the content of syringin and alkynin of radix codonopsis is characterized by comprising the following steps:
(1) magnetizing the fully-granulated codonopsis pilosula seeds for 710-790 s, immersing the codonopsis pilosula seeds in a cobalt oxalate solution for 32-36 min, continuously stirring, taking out filtrate, and draining until no water drops drop to obtain the processed codonopsis pilosula seeds;
(2) sowing the treated codonopsis pilosula seeds in seedling raising holes of a seedling raising substrate, sowing one seed in each hole, wherein the sowing depth is 1.2-1.4 cm, and transplanting after seedling emergence for 40-45 days;
the seedling raising substrate is prepared from the following raw materials in parts by weight: 34-38 parts of grape skin residues, 21-23 parts of tomato seed meal, 15-17 parts of purple sweet potato vine, 8-9 parts of acacia flower, 110-120 parts of pond sludge, 8-9 parts of activated medical stone powder, 0.12-0.14 part of andrographolide, 0.16-0.18 part of chlorogenic acid and 0.12-0.14 part of chufa polysaccharide;
the magnetization treatment in the step (1) comprises the steps of performing magnetization treatment for 100-120 s under the condition that the magnetic field intensity is 1120-1140G, pausing for 30-40 s, performing magnetization treatment for 190-210 s under the condition that the magnetic field intensity is 640-660G, pausing for 50-60 s, and performing magnetization treatment for 340-360 s under the condition that the magnetic field intensity is 640-660G;
the cobalt oxalate solution in the step (1) is prepared according to the following steps: putting 1.7-1.9 parts by weight of cobalt oxalate and 0.22-0.24 part by weight of mesona polysaccharide into 240-260 parts by weight of water, and stirring until the cobalt oxalate solution is dissolved to obtain a cobalt oxalate solution;
the activated medical stone powder in the step (2) is prepared according to the following steps: crushing medical stone, putting the crushed medical stone into a rotary frying pan with the temperature of 102-108 ℃ for constant-temperature frying for 28-30 min, taking out, grinding the medical stone into powder, putting the powder into lithium hydroxide aqueous solution with the weight of 0.5-0.6 times of the powder and the mass concentration of 4.4-4.8%, uniformly stirring the solution, carrying out ultrasonic treatment for 14-16 min under the conditions of the power of 160-170W and the frequency of 96-100 kHz, and carrying out spray drying at the temperature of 71-75 ℃ to obtain activated medical stone powder;
the preparation method of the seedling substrate in the step (2) specifically comprises the following steps:
A. mixing and crushing grape skin residues, tomato seed meal, purple sweet potato vines and acacia flowers, performing constant-temperature steam treatment for 13-15 min at the temperature of 90-94 ℃, performing constant-temperature steam treatment for 21-25 min at the temperature of 141-149 ℃, putting into a fermentation tank at the temperature of 43-45 ℃ for constant-temperature fermentation for 140-160 h, and taking out to obtain fermentation pulp;
B. uniformly mixing and stirring the fermentation pulp and the pond sludge, covering a plastic film, fermenting at a constant temperature of 37-39 ℃ for 280-300 h, uniformly mixing and stirring with activated medical stone powder, andrographolide, chlorogenic acid and water chestnut polysaccharide, and adjusting the water content to 75-80% to obtain the seedling culture substrate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810543861.2A CN108739178B (en) | 2018-05-31 | 2018-05-31 | Sowing and seedling raising method for increasing content of syringin and alkynin of codonopsis pilosula |
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| CN201810543861.2A CN108739178B (en) | 2018-05-31 | 2018-05-31 | Sowing and seedling raising method for increasing content of syringin and alkynin of codonopsis pilosula |
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| CN108739178A CN108739178A (en) | 2018-11-06 |
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| CN109156314A (en) * | 2018-11-22 | 2019-01-08 | 遵义驰宇御园生态农业开发有限公司 | A kind of paddy rice in cold region seedling medium |
| CN112772332B (en) * | 2021-01-15 | 2022-11-22 | 湖北省农业科学院中药材研究所 | Seedling growing and planting method for codonopsis pilosula in Banqiao mode |
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| CN102701872A (en) * | 2012-06-30 | 2012-10-03 | 刘春燕 | Solid fertilizer |
| CN105706716A (en) * | 2016-03-04 | 2016-06-29 | 宋维成 | Planting method for increasing lobetyolin content |
| CN107711357A (en) * | 2017-10-13 | 2018-02-23 | 陈增光 | A kind of method for culturing seedlings for improving Black Rice general flavone and anthocyanin content |
| CN107889577A (en) * | 2017-12-22 | 2018-04-10 | 枞阳县新农民农业种植有限公司 | A kind of method for treating seeds for improving Pseudostellaria Polysaccharide and saponin content |
| CN107912266A (en) * | 2017-12-12 | 2018-04-17 | 凤阳县板桥镇金祥种植专业合作社 | A kind of seedling medium for strengthening tomato disease resistance |
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| CN207054041U (en) * | 2017-01-10 | 2018-03-02 | 银川广益达节能科技有限公司 | A kind of seed magnetization device |
| CN107382477A (en) * | 2017-07-31 | 2017-11-24 | 安徽华联肥业有限公司 | A kind of microbial immobilized organic fertilizer and preparation method thereof |
| CN107567844A (en) * | 2017-09-26 | 2018-01-12 | 界首市东方果源家庭农场 | A kind of cuttage and seedling culture method of high-quality chrysanthemum |
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| CN102701872A (en) * | 2012-06-30 | 2012-10-03 | 刘春燕 | Solid fertilizer |
| CN105706716A (en) * | 2016-03-04 | 2016-06-29 | 宋维成 | Planting method for increasing lobetyolin content |
| CN107711357A (en) * | 2017-10-13 | 2018-02-23 | 陈增光 | A kind of method for culturing seedlings for improving Black Rice general flavone and anthocyanin content |
| CN107912266A (en) * | 2017-12-12 | 2018-04-17 | 凤阳县板桥镇金祥种植专业合作社 | A kind of seedling medium for strengthening tomato disease resistance |
| CN107889577A (en) * | 2017-12-22 | 2018-04-10 | 枞阳县新农民农业种植有限公司 | A kind of method for treating seeds for improving Pseudostellaria Polysaccharide and saponin content |
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