CN108738950A - The biological control method of random tarsonemid - Google Patents
The biological control method of random tarsonemid Download PDFInfo
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- CN108738950A CN108738950A CN201810784702.1A CN201810784702A CN108738950A CN 108738950 A CN108738950 A CN 108738950A CN 201810784702 A CN201810784702 A CN 201810784702A CN 108738950 A CN108738950 A CN 108738950A
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- mite
- pasteur
- tarsonemid
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 22
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 8
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 8
- 241000238631 Hexapoda Species 0.000 claims abstract description 8
- 210000004681 ovum Anatomy 0.000 claims abstract description 8
- 235000011430 Malus pumila Nutrition 0.000 claims description 10
- 235000015103 Malus silvestris Nutrition 0.000 claims description 10
- 239000002480 mineral oil Substances 0.000 claims description 9
- 235000010446 mineral oil Nutrition 0.000 claims description 9
- 239000000839 emulsion Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 7
- 239000012895 dilution Substances 0.000 claims description 7
- DTDSAWVUFPGDMX-UHFFFAOYSA-N spirodiclofen Chemical compound CCC(C)(C)C(=O)OC1=C(C=2C(=CC(Cl)=CC=2)Cl)C(=O)OC11CCCCC1 DTDSAWVUFPGDMX-UHFFFAOYSA-N 0.000 claims description 7
- XGWIJUOSCAQSSV-XHDPSFHLSA-N (S,S)-hexythiazox Chemical compound S([C@H]([C@@H]1C)C=2C=CC(Cl)=CC=2)C(=O)N1C(=O)NC1CCCCC1 XGWIJUOSCAQSSV-XHDPSFHLSA-N 0.000 claims description 6
- 239000005661 Hexythiazox Substances 0.000 claims description 6
- 230000000895 acaricidal effect Effects 0.000 claims description 6
- 230000000749 insecticidal effect Effects 0.000 claims description 6
- WCXDHFDTOYPNIE-RIYZIHGNSA-N (E)-acetamiprid Chemical compound N#C/N=C(\C)N(C)CC1=CC=C(Cl)N=C1 WCXDHFDTOYPNIE-RIYZIHGNSA-N 0.000 claims description 5
- 239000005875 Acetamiprid Substances 0.000 claims description 5
- 239000005906 Imidacloprid Substances 0.000 claims description 5
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 claims description 5
- 229940056881 imidacloprid Drugs 0.000 claims description 5
- 244000141359 Malus pumila Species 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- -1 mite dead net Chemical compound 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 claims 2
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 claims 2
- 244000081841 Malus domestica Species 0.000 claims 1
- 210000000481 breast Anatomy 0.000 claims 1
- 238000007599 discharging Methods 0.000 claims 1
- 231100000053 low toxicity Toxicity 0.000 claims 1
- 239000002420 orchard Substances 0.000 abstract description 6
- 239000000575 pesticide Substances 0.000 abstract description 6
- 230000009467 reduction Effects 0.000 abstract description 3
- 244000062804 prey Species 0.000 abstract 1
- 241000220225 Malus Species 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 206010038743 Restlessness Diseases 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 241001164374 Calyx Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 241000238876 Acari Species 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241001206567 Neoseiulus barkeri Species 0.000 description 1
- 241001658757 Neoseiulus californicus Species 0.000 description 1
- 241000916145 Tarsonemidae Species 0.000 description 1
- 241000344246 Tetranychus cinnabarinus Species 0.000 description 1
- 150000001409 amidines Chemical class 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100001143 noxa Toxicity 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000003359 percent control normalization Methods 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The present invention provides a kind of biological control method of random tarsonemid, is to discharge the new small peaceful mite of Pasteur on fruit tree to be used as natural enemy, preys on the ovum of random tarsonemid, young mite, quiescent stage mite or at mite, progress control of insect.The present invention has stronger prey ability using the new small peaceful mite of Pasteur to random tarsonemid, biological control is carried out to random tarsonemid using Pasteur new small peaceful mite, Pesticide use amount can be greatly reduced, significantly improve fruit quality, not only meet the policy that national pesticide reduction uses, but also increase the income of orchard worker.
Description
Technical field
The present invention relates to technical field of biological control, specifically, being related to the biological control method of random tarsonemid.
Background technology
Since bagging technique is promoted, successively on Gansu, Shaanxi, Liaoning, Shandong, Hebei, Yunnan and other places, generally occur
" Apple with Fruit-bagged Treatment calyx low-lying area diplostomiasis ", incidence less serious case 5~6%, severe one reaches 60% or even 90%, averagely reach 20% with
On.Heavy economic losses is caused to Fruits production and orchard worker.Random tarsonemid (Tarsonemus confusus Ewing) is subordinate to
The true mite catalogue Acariformes Tarsonemidaes Tarsonemidae Tarsonemus Tarsonemus of Acari Acari, are demonstrate,proved
It is to lead to pathogenetic cause noxa of Apple with Fruit-bagged Treatment stain in fact, is distributed widely in all over the world.Since random tarsonemid is in apple set
Enter fruit bag after bag, settles down in fruit calyx portion, endangered in the feeding of calyx depression, there is very strong concealment, difficulty of prevention and cure is larger, is
The insect pest is controlled, a large amount of multiple applications pesticides not only increase cost accounting, while also polluted environment, brought to fruit
Security risk.
The new small peaceful mite (Neoseiulus barkeri Hughes) of Pasteur is polyphagy predator, is distributed widely in the world
Various regions, major part provinces and cities of China are distributed.
Invention content
The object of the present invention is to provide a kind of biological control methods of random tarsonemid.
It is a further object of the present invention to provide new application of the small peaceful mite in anti-eliminate-disorder tarsonemid of Pasteur.
It is on fruit tree to realize that the object of the invention, the present invention provide a kind of biological control method of random tarsonemid
It discharges the new small peaceful mite of Pasteur and is used as natural enemy, prey on the ovum of random tarsonemid, young mite, quiescent stage mite or at mite, progress control of insect.
The present invention was found by experiment that the new small peaceful mite of Pasteur in 24 hours, the ovum of independent feeding unrest tarsonemid, young mite, quiet
Breath phase mite or at mite, highest is shown respectively up to 40,50,32 and 36 to the stronger predation energy of random tarsonemid
Power.
The biological control method of unrest tarsonemid provided by the invention, the release index of the new small peaceful mite of Pasteur：When random tarsonemid
The burst size of insect population≤2/blinds, the new small peaceful mite of Pasteur is：When trunk diameter >=10cm, according to burst size (head)=
375+62.5 × Φ is discharged, and when trunk diameter < 10cm, according to the tree crown size of each tree, discharges 500-1000 heads.
Wherein, Φ is trunk diameter, measure position be butt away from ground 10cm at, unit cm.
Fruit tree of the present invention includes but not limited to apple tree.According to the method described above, Predatory Mites are discharged before Apple Bags.
Can effectively anti-eliminate-disorder tarsonemid, reduce the generation of diplostomiasis.
Preferably, before the new small peaceful mite of release Pasteur, can according to insect pest situation first with less toxic Insecticidal and acaricidal agent spray by
The fruit tree of random tarsonemid harm.
The Insecticidal and acaricidal agent is in mineral oil emulsion, imidacloprid, Acetamiprid, mite dead net, Hexythiazox, Envidor etc.
At least one.
The spraying concentration of the Insecticidal and acaricidal agent is：It is 200 times of dilutions that mineral oil emulsion, which is used alone,；Imidacloprid, pyridine
It is that 1000-2000 times of dilution or by specification use that worm amidine, mite dead net, Hexythiazox, Envidor, which are used alone,；Mineral oil emulsion
When being used in mixed way with imidacloprid or Acetamiprid or the dead net or Hexythiazox of mite or Envidor, mineral oil emulsion is 400-500 times and dilutes
Liquid, remaining is used for 1000-2000 times of dilution or by specification.
The present invention also provides new application of the small peaceful mite in anti-eliminate-disorder tarsonemid of Pasteur.
By above-mentioned technical proposal, the present invention at least has following advantages and advantageous effect：
The present invention uses the new small peaceful mite of Pasteur to carry out biological control, before bagging, random tarsonemid worm to random tarsonemid for the first time
When mouth radix≤2/blinds, whole year only needs 1 Pasteur of release, and newly small peaceful mite can effectively reduce the generation of diplostomiasis, disease fruit
Rate can be controlled 2% hereinafter, the case where avoiding orchard worker blindly specific aim for several times uses pesticide, not only significantly improves fruit
Quality has ensured fruit safety, has increased the economic well-being of workers and staff of orchard worker, also is compliant with national pesticide reduction and uses and ensure food
The policy of safety, there is significant social benefit and ecological benefits.
Description of the drawings
Fig. 1 is the new small peaceful mite of Pasteur in the embodiment of the present invention 1 to the Selective preying effect of random tarsonemid.
Specific implementation mode
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
Used in the conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
The biological control method (laboratory test) of the random tarsonemid of embodiment 1
1, material to be tested
For the new small peaceful mite of examination natural enemy Pasteur, Hebei province Changli County is picked up from, interior carries out continuously rearing using flour mite as food.
Random tarsonemid is picked up from the apple tree of Hebei province Changli County.
Test cell setting：Experiment cell is made of lower layer's poly (methyl methacrylate) plate and upper layer glass slide.Poly (methyl methacrylate) plate length and width
Height is respectively 50mm, 25mm and 5mm, and beats the circular hole of diameter 16mm in its centre, and depth 4mm forms the small of certain space
Room；Glass slide length, width and height are respectively 50mm, 25mm and 1mm, prevent experiment from being escaped with worm as lid.When experiment, small interior drop
Enter a drop PDA culture medium, to keep small indoor humidity, is put into test worm, covers glass slide and clamped with binder clip.
2, test method
2.1 predation Choice tests
The ovum into random tarsonemid, young mite, quiescent stage and each 5 of adult are chosen in same small interior, then to accessing 1 in cell
The new small peaceful mite of the hungry Pasteur for 24 hours of head is female at mite, observes and records small new neoseiulus californicus different mite states each to Tetranychus cinnabarinus afterwards for 24 hours
Predatory number, be repeated 20 times.
Day predatory number of the new small peaceful mite of 2.2 Pasteur to each worm state of random tarsonemid
With fine, soft fur pen by the ovum of random tarsonemid, young mite, quiescent stage and it is female at mite each 50 be respectively connected to test small interior, then
The new small peaceful mite of the Pasteur of 1 starvation for 24 hours is accessed into cell.Observe and record the quantity of prey afterwards for 24 hours, often processing is repeated 5 times.
3, result and analysis
Preference of the new small peaceful mite of 3.1 Pasteur to random tarsonemid difference mite state
From figure 1 it appears that in the case where four kinds of mite states exist simultaneously, the new small peaceful mite most eating unrest tarsonemid of Pasteur
Young mite, according to significance difference analysis, the prey ratio highest of young mite, be 0.42a, be significantly higher than other worm states, other
It is followed successively by female at mite 0.23b, quiescent stage 0.19bc, ovum 0.16c.
Predation functional responses of the new small peaceful mite of 3.2 Pasteur to random tarsonemid
From table 1 it follows that under non-selective conditions, the new small peaceful mite of Pasteur has each mite state of random tarsonemid larger
Predatory number.Under this experimental condition, the new small peaceful mite of Pasteur is in 24 hours, the ovum of independent feeding unrest tarsonemid, young mite, quiescent stage
Or at mite, highest is shown respectively up to 40,50,32 and 36 to the stronger prey ability of random tarsonemid.
Predatory number of the new small peaceful mite of 1 Pasteur of table to each worm state of random tarsonemid
It these results suggest that the new small peaceful mite of Pasteur can prey on the various worm states of random tarsonemid, and predatory number is larger, it can be with
The effectively population quantity of the random tarsonemid of control.
The biological control method (field trial) of the random tarsonemid of embodiment 2
The apple orchard of diplostomiasis often occurs for selection Changli County, and 20 days before fruit tree bag cover (on May 18th, 2017), selection makes
Full garden spraying is carried out with the 4000 times of liquid mixing of 400 times of liquid of mineral oil emulsion and Envidor, makes the reduction of evil mite population number, avoids releasing
It puts day enemy's rear area and uses pesticide.10 apple trees (average diameter of trunk is about 18cm) of selection in orchard, on June 2nd, 2017,
Check random tarsonemid population number within 5 days before bagging.Random tarsonemid insect population≤2/blinds then randomly chooses 5 and sets, every
The new small peaceful mite 1500 of tree release Pasteur, 5 trees of control are without any processing.The generation feelings of diplostomiasis are checked when fruit harvest
Condition.As a result display releases the fruit tree diplostomiasis diseased fruit rate 1.5% of the new small peaceful mite of Pasteur, and the fruit tree diseased fruit rate not discharged is up to
15.5%, illustrate to discharge the new small peaceful mite of Pasteur can effectively anti-eliminate-disorder tarsonemid, control the generation of diplostomiasis.
Field control effect of the new small peaceful mite of 2 Pasteur of table to random tarsonemid (diplostomiasis)
| Test process | Diplostomiasis diseased fruit rate (%) |
| Discharge 1500/ | 1.5% |
| Control | 15.5% |
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be modified or is improved, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. the biological control method of random tarsonemid, which is characterized in that discharge the new small peaceful mite of Pasteur on fruit tree as natural enemy, predation
The ovum of random tarsonemid, young mite, quiescent stage mite or at mite, carry out control of insect.
2. according to the method described in claim 1, it is characterized in that, the release index of the new small peaceful mite of Pasteur：When random tarsonemid worm
The burst size of mouthful radix≤2/blinds, the new small peaceful mite of Pasteur is：When trunk diameter >=10cm, according to release head number=375+
62.5 × Φ is discharged；When trunk diameter < 10cm, according to the tree crown size of each tree, 500-1000 heads are discharged.
Wherein, Φ is trunk diameter, measure position be butt away from ground 10cm at, unit cm.
3. according to the method described in claim 1, it is characterized in that, the fruit tree is apple tree.
4. according to the method described in claim 3, it is characterized in that, discharging the new small peaceful mite of Pasteur before Apple Bags.
5., can be according to insect pest situation elder generation according to the method described in claim 1, it is characterized in that, before the new small peaceful mite of release Pasteur
The fruit tree endangered by random tarsonemid is sprayed with the Insecticidal and acaricidal agent of low toxicity.
6. according to the method described in claim 5, it is characterized in that, the Insecticidal and acaricidal agent is selected from mineral oil emulsion, pyrrole worm
At least one of quinoline, Acetamiprid, mite dead net, Hexythiazox, Envidor.
7. according to the method described in claim 6, it is characterized in that, the spraying concentration of the Insecticidal and acaricidal agent is：Mineral oil breast
It is 200 times of dilutions that agent, which is used alone,；It is 1000-2000 that imidacloprid, Acetamiprid, mite dead net, Hexythiazox, Envidor, which are used alone,
Times dilution or by specification use；Mineral oil emulsion is mixed with imidacloprid or Acetamiprid or the dead net or Hexythiazox of mite or Envidor
In use, mineral oil emulsion is 400-500 times of dilution, remaining is used for 1000-2000 times of dilution or by specification.
8. new application of the small peaceful mite in anti-eliminate-disorder tarsonemid of Pasteur.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810784702.1A CN108738950B (en) | 2018-07-17 | 2018-07-17 | Biological control method for Tarsonemus confusus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810784702.1A CN108738950B (en) | 2018-07-17 | 2018-07-17 | Biological control method for Tarsonemus confusus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108738950A true CN108738950A (en) | 2018-11-06 |
| CN108738950B CN108738950B (en) | 2021-07-06 |
Family
ID=63970426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810784702.1A Active CN108738950B (en) | 2018-07-17 | 2018-07-17 | Biological control method for Tarsonemus confusus |
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| Country | Link |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112154832A (en) * | 2020-09-23 | 2021-01-01 | 北京农生科技有限公司 | Safe use method of neoseiulus californicus |
| CN113647283A (en) * | 2021-09-10 | 2021-11-16 | 潍坊学院 | Biological control method for tetranychidae vegetables |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112154832A (en) * | 2020-09-23 | 2021-01-01 | 北京农生科技有限公司 | Safe use method of neoseiulus californicus |
| CN113647283A (en) * | 2021-09-10 | 2021-11-16 | 潍坊学院 | Biological control method for tetranychidae vegetables |
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| Publication number | Publication date |
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| CN108738950B (en) | 2021-07-06 |
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Inventor after: Yu Lichen Inventor after: Li Litao Inventor after: Jiao Rui Inventor after: He Limin Inventor after: Xu Changxin Inventor after: Zhang Linlin Inventor after: Liu Jinli Inventor before: Yu Lichen |
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