CN107027784A - A kind of compounded pesticides and its application containing nimbin and double third ring worm esters - Google Patents
A kind of compounded pesticides and its application containing nimbin and double third ring worm esters Download PDFInfo
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- CN107027784A CN107027784A CN201710390129.1A CN201710390129A CN107027784A CN 107027784 A CN107027784 A CN 107027784A CN 201710390129 A CN201710390129 A CN 201710390129A CN 107027784 A CN107027784 A CN 107027784A
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- nimbin
- ring worm
- compounded pesticides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of compounded pesticides containing nimbin and double third ring worm esters and its application, its active ingredient is nimbin and double third ring worm esters, and the quality proportioning of nimbin and double third ring worm esters is 1:1~1:100.The invention also provides a kind of application containing nimbin and double third ring worm ester compounded pesticides, nimbin and double third ring worm ester composite usages are diluted into the sucking pests such as 600~1000 times of preventing and treating aleyrodids, aphid, fleahoppers in sucking pests such as preventing and treating aleyrodid, aphid, fleahoppers there is provided the double third ring worm ester fields of 1.8%~3.6% nimbin.Compounded pesticides and its application that the present invention is used, reduce the dosage of agricultural chemicals, reduce pollution of the agricultural chemicals to environment, effectively delay the resistance to the action of a drug to single medicament, are conducive to environmental protection and the comprehensive regulation of agricultural pests.
Description
Technical field
The invention belongs to technical field of pesticide, more particularly to a kind of compounded pesticides containing nimbin and double third ring worm esters and
It is applied, and the compounded pesticides are used to prevent and treat the sucking pests such as aleyrodid, aphid, fleahopper,.
Background technology
Nimbin, English name：Azadirachtin, is a kind of compound that chinaberry is printed from plant, has to insect and refuse
Food, stomach toxicity, the effect grown of tagging and suppress, its mechanism of action are the endocrines for upsetting insect.Nimbin has excellent
Insecticidal activity, it is almost all effective to all agricultural pests, and consumption is low, degrade in the environment fast, do not injure natural enemy and its
Its non-target organism, is the generally acknowledged most promising botanical biological pesticide of improvement insect in the whole world.
Double third ring worm esters, English name：Afidopyropen, is Japanese Meiji Pharmaceutial Ltd. and Japanese North research
A kind of novel pesticide of institute's joint research exploitation, with brand-new mechanism of action, to sucking pest such as plant hopper, aphid, Jie
Shell worm and leafhopper are respectively provided with good lethal effect, available for vegetables, fruit tree, imtertilled crop and ornamental plant etc., with wide
Application prospect.
The long-term, a large amount of, single of chemical pesticide uses in agricultural production, in addition abuse, cause produce pest resistance to insecticide,
Environmental pollution, residues of pesticides, bring increasingly serious pollution to whole ecological environment and human health and cause harm.Therefore, grind
Study carefully the efficient compounded pesticides kind of exploitation turns into the task of top priority.
The research active on nimbin, double third ring worm ester biologicals is it has been reported that but at present it is not yet found that closing nimbin
Research with the compounded pesticides of double third ring worm esters is reported.
The content of the invention
For the vacancy of the existing compounded pesticides for closing nimbin and double third ring worm esters in the prior art, the purpose of the present invention
It is to provide a kind of compounded pesticides containing nimbin and double third ring worm esters and its application, the compounded pesticides contain plant source agriculture
Medicine, it is environmentally friendly to people, animal low toxicity, the generation of pest resistance to insecticide can be slowed down, and by the compounded pesticides sheet in preventing and treating powder
Applied in terms of the sucking pests such as lice, aphid, fleahopper.
To solve problems of the prior art, the present invention provides a kind of compounding containing nimbin and double third ring worm esters
Insecticide, it is characterized in that：Compounded pesticides main component is nimbin and double third ring worm esters, nimbin and double third ring worms
The mass ratio of ester is 1:1~1:100.
It is used as a kind of optimal way：A kind of compounded pesticides containing nimbin and double third ring worm esters, it is characterised in that print
The mass ratio of chinaberry element and double third ring worm esters is 1:1~1:60.
It is used as another optimal way：A kind of compounded pesticides containing nimbin and double third ring worm esters, it is characterised in that
The mass ratio of nimbin and double third ring worm esters is 1:1~1:20.
It is used as another optimal way：A kind of compounded pesticides containing nimbin and double third ring worm esters, it is characterised in that
The mass ratio of nimbin and double third ring worm esters is 1:1~1:10.
It is used as a kind of more optimal way：A kind of compounded pesticides containing nimbin and double third ring worm esters, it is characterised in that
The mass ratio of nimbin and double third ring worm esters is 1:5.
Nimbin and double mass percents of the third ring worm ester in whole compounded pesticides are respectively nimbin in the present invention
0.3~0.6%, double third ring worm esters 1.8~3%, surplus is customary adjuvant additive.
The present invention also provides a kind of compounded pesticides application containing nimbin and double third ring worm esters, it is characterised in that：Institute
Stating compounded pesticides is used for controlling sucking insects.
It is used as a kind of optimal way：Compounded pesticides containing the double third ring worm esters of 1.8%~3.6% nimbin are used for
Field dilutes 600~1000 times of controlling sucking insects.
It is used as another optimal way：Compounded pesticides containing the double third ring worm esters of 3% nimbin are used for field
Dilute 600~1000 times of controlling sucking insects.
The compounded pesticides of the present invention are screened and field control effectiveness test through series of indoor, and selective advance significantly compounds ratio
Row, have quick-acting and lasting effect concurrently, and the key point that preventive effect is the present invention is improved while reducing Pesticide use amount.
Compared with prior art, its advantage is the present invention：
1st, there is obvious synergistic effect after nimbin and double third ring worm esters are compound：Both nimbin and double third ring worm esters with
1:1 to 1:Show to be added or synergistic effect in 100 ratio range, the mass ratio of wherein nimbin and double third ring worm esters is
5:When 1, co-toxicity coefficient reaches 558.46, has embodied extremely high-caliber synergistic effect.
2nd, the usage amount of agricultural chemicals is reduced：Field efficacy is not only increased after nimbin and double third ring worm ester compoundings, and
The compounded pesticides of nimbin and double third ring worm esters reduce 58.50% and more than 16.60% usage amount compared with two single doses respectively.
The multiple insecticide reasonable mixture ratio of the present invention, the virulence to sucking pests such as Aleurocanthus spiniferus is stronger, co-toxicity coefficient compared with
Height, and have stronger synergistic effect, it is the built agent of a class high-efficiency low-toxicity.
Embodiment
The embodiment of the present invention is provided by taking tea tree Aleurocanthus spiniferus as an example below, for technical scheme
It is described further, but the present invention is only limited to absolutely not following examples.Percentage is mass percent in formula, is used
Medicine is the purchase of other producers.
A kind of compounded pesticides that the present invention is provided are the double third ring worm ester compounded pesticides of nimbin, the compound insecticidal
Containing nimbin and double third ring worm esters in the active ingredient of agent, the quality proportioning of nimbin and double third ring worm esters is 1:1~1:
100。
In the compounded pesticides of the present invention, the quality proportioning of nimbin and double third ring worm esters is preferably 1:1~1:60.
In the compounded pesticides of the present invention, the quality proportioning of nimbin and double third ring worm esters is preferably 1:1~1:20.
In the compounded pesticides of the present invention, the quality proportioning of nimbin and double third ring worm esters is preferably 1:1~1:10.
In the compounded pesticides of the present invention, the quality proportioning of nimbin and double third ring worm esters is preferably 1:5.
The invention also provides a kind of application of compounded pesticides, using above-mentioned compounded pesticides, for prevent and treat aleyrodid,
The sucking pests such as aphid, fleahopper.
Preferably, by the double third ring worm ester compounded pesticides of 1.8%~3.6% nimbin, field dilutes 600~1000 times
Prevent and treat tea tree Aleurocanthus spiniferus.
Preferably, by the double third ring worm ester compounded pesticides of 3% nimbin, field dilutes 600~1000 times of preventing and treating tea trees
Aleurocanthus spiniferus.
The present invention is right after botanical pesticide nimbin and the double third ring worm esters exclusive uses of chemical pesticide and compounding by studying
The indoor bioassay and field test of Aleurocanthus spiniferus, the characteristics of evaluating both agricultural chemicals, and then by rationally compounding, inquire into print
The prevention effect of chinaberry element and double third ring worm ester compoundings.
Experiment one：Indoor bioassay is tested
The test method used in the experiment one is specifically divided into following steps：
Step 1: medicament is prepared
Selected medicament is：1% micro nimbin emulsion, the medicament is provided by Guangdong vegetable garden bioengineering Co., Ltd；
5% pair of the third ring worm ester dispersible agent, the medicament is provided by BASF AG, when making compounded pesticides, nimbin with it is double
The mass ratio of third ring worm ester chooses 1 respectively:1,1:5,1:10,1:20,1:40,1:60,1:80,1:100.It is made into acetone dilution
10000mg/L mother liquors are standby, and nimbin, double third ring worm esters and its each compound proportion are diluted to 6 concentration gradients respectively.
Step 2: selected insect source and assay method
Supply and marketing cooperative of Yantai City dragon's fountain tea place, tea tree tender leaf of the collection with Aleurocanthus spiniferus nymph are picked up from experiment with Aleurocanthus spiniferus
Take back indoor standby.
Sensitiveness of the Aleurocanthus spiniferus to insecticide is determined using blade infusion process.Each reagent agent is diluted to 6 concentration
Gradient, chooses the light green tealeaves blade with Aleurocanthus spiniferus nymph, the first 3 age nymph of microscopy 50, is cleared away with writing brush under the microscope
Unnecessary or dead nymph.Leaf is impregnated into 5s in the decoction prepared in advance, takes out and dries, be put into culture dish.Often handle
It is repeated 4 times, totally 200 nymphs, using clear water as control.
Step 3: raising and observation
Aleurocanthus spiniferus after processing be placed in temperature be 25 DEG C ± 1 DEG C, relative humidity 60%, photoperiod be L:D=(16:8)h
Illumination box in raise and observe.
Step 4: result inspection
Nymph death condition is checked after 48h under the microscope, with polypide it is shrivelled, change colour as death standard, record total respectively
Borer population and dead borer population.
Step 5: data processing
Bioassay results carry out statistical analysis using the analysis softwares of SPSS 20.0, and the poison of surveyed medicament is calculated respectively
Power regression equation, LC50 and coefficient correlation.
The desinsection for evaluating nimbin and double third ring worm ester different ratio insecticides using Sun Peiyun etc. co-toxicity coefficient method is lived
Property.
Toxicity index TI=(the LC of standard agent50The LC of/reagent agent50)×100
The actual toxicity index of mixture (ATI)=(LC of standard agent50The LC of/mixture50)×100
Mixture theoretical toxicity index (TTI)=[TI × A agent of A agent percentage (%) shared in mixture]+[B agent
The percentage (%) that TI × B agent is accounted in mixture]
Co-toxicity coefficient (CTC)=[the actual toxicity index of mixture (ATI)]/[mixture theoretical toxicity index (TTI)] × l00
According to the regulation of the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC, the criterion of mixture formula synergy is：Co-toxicity coefficient is more than 120
Synergistic effect, co-toxicity coefficient 80-120 is summation action, and it is antagonism that co-toxicity coefficient, which is less than 80,.The selection of optimum proportioning is with mixed
The co-toxicity coefficient and LC of agent50(or LD50) value two indices weigh, i.e., co-toxicity coefficient is higher and LC50(or LD50) value minimum is (i.e.
Survey toxicity index maximum).
Step 6: test results and analysis
The nimbin of table 1 virulence of the compounding than arranging to Aleurocanthus spiniferus 3 age nymph different with double third ring worm esters
From table 1 it follows that in different ratio mixtures, mass ratio is 1:80 and 1:100 coefficient of spending together is 80
It is summation action between~120.The coefficient of spending together of other proportionings is all higher than 120, shows certain synergistic effect, wherein common
The maximum proportioning of malicious coefficient is 1:5, co-toxicity coefficient has reached 558.46.Therefore, choose and major in that coefficient is higher and LC50Value minimum
Match (nimbin:Double third ring worm ester=1:5) complex preparation of corresponding proportion is made, and has carried out field medicine effect test and evaluates it
Field efficacy situation.
Experiment two, field efficacy evaluation test
The nimbin gone out in the experiment according to lab screening and double third ring worm ester optimum compound proportion examples 1:5 carry out field medicine
Effect experiment, is made effect preferable 1.8%, 2.4%, 3%, the double third ring worm ester compounded pesticides of 3.6% nimbin, through analysis
It was found that in the insecticide of above-mentioned various concentrations, wherein best with 3% preventive effect.Therefore, field is with double third rings of 3% nimbin
Exemplified by 600 times, 800 times, 1000 times of worm ester compounded pesticides (containing 0.5% nimbin and 2.5% pair of the third ring worm ester), with 1% print
500 times of chinaberry element microemulsion, 5% pair of the third ring worm ester dispersible agent 1000 carry out field control effectiveness test again as comparison medicament.
The test method used in the experiment two is specifically divided into following steps：
Step 1: experimental condition
Subjects are Aleurocanthus spiniferus Aleurocanthus spiniferus (Quaintanca), are for examination tea tree breed
Pingyang is special early.
Experimental field use Protectorate cultivation, be the young tea plantations that bud slightly grows densely, Aleurocanthus spiniferus over the years occur it is universal and
Uniformly.The cultivation condition (soil types, fertilising, growing stage, seeding row spacing etc.) and plant growing way of all experimental plots are homogeneous, and
It is consistent with local cultivation step.
Step 2: test method
In tea place Aleurocanthus spiniferus occurance peak dispenser on July 13 once, Su Nong 3WH-36 type high pressure power driven sprayers are utilized
Carry out complete stool spraying, with blade tow sides it is uniform medicine, untill dripping.Actual mu amount of liquid medicine is 100 liters/mu.Per cell
30m2, random alignment, often 4 repetitions of processing.
Step 3: investigation, record and computational methods
(July 13) investigates insect population before medicine, if investigating the borer population on 100 blades per cell, and is made of marking pen
Mark, 3 days after medicine, 7 days, the work borer population of each investigation in 15 days once on institute's index blade, is investigated 4 times altogether.
Drug effect computational methods are as follows：
Prevention effect (%)=prevention effect (%)=[1- (borer population living after borer population × treatment region medicine living before the medicine of check plot)/
Borer population living before borer population × treatment region medicine living after the medicine of check plot)] × 100
Multiple range test is carried out to each preventive effect using the Duncan duncan's new multiple range methods of DPSv7.05 data processing softwares.
Step 4: test results and analysis
Result of the test can be seen that by 1 from table 2:The double third ring worm esters of 3% nimbin that 5 mass ratio is made into are answered
There is good field efficacy to tea tree Aleurocanthus spiniferus nymph with insecticide, its quick-acting and lasting effect are above two kinds of single doses.
Wherein, 3 days after 600 times of compounded pesticides processing medicines, 7 days, the preventive effect of 15 days be respectively 87.25%, 93.48%, 91.58%,
It is significantly higher than two kinds of single doses.Therefore, by taking 3% nimbin, 600 times of processing of double third ring worm ester compounded pesticides as an example, calculation specifications
The characteristics of complex preparation reduces pesticide dosage compared with single dose.
In addition, each chemicals treatment is safe without poisoning to confession examination tea tree breed during experiment, and the compounded pesticides are to tea place
Natural enemy insect quantity has no significant effect.
The field efficacy of the complex preparation of table 2 and each single dose to tea tree Aleurocanthus spiniferus nymph
Note：Data are to manage average data everywhere in table, and different letters represent significant difference (P in same column<0.05).
As shown in table 3, in the case of higher than two kinds single doses of prevention effect, the double third ring worm ester compoundings of 3% nimbin are killed
The dosage of 600 times of processing of worm agent is still less than each single dose, is reduced compared with 1% micro nimbin emulsion, 500 times of processing dosages
58.50%, 16.60% dose, compounded pesticides pesticide reduction are reduced again compared with 5% pair of the third ring worm ester dispersible agent 1000
Substantially.
The compounded pesticides decrement Medicinal Trait of table 3
Can also be using experiment two the step of, the double third ring worm esters of nimbin that the concentration from other mass ratioes is 3%
Compounded pesticides, or other mass concentration ratios are 1.8%, 2.4%, the 3.6% double third ring worm ester compounded pesticides of nimbin
Field efficacy evaluation test is carried out, the double third ring worm ester compounded pesticides of nimbin of the various concentrations of other mass ratioes are found
There is good prevention effect respectively, but the best still mass ratio of performance is 1:The nimbin double third that 5 concentration is 3%
Ring worm ester compounded pesticides.
In summary, the present invention is increased using two kinds of agricultural chemicals nimbins and the compounded pesticides of double third ring worm esters with collaboration
Effect is acted on, and compared with existing unitary agent, insecticidal effect is significantly improved, and pesticide dosage is reduced, and to crop safety, is added
Prevent and treat the selectivity of the sucking pest medicaments such as aleyrodid, aphid, fleahopper.
To those skilled in the art, part combination of the above only to the present invention is illustrated, it is clear that this hair
The bright details for being not limited to above-mentioned one exemplary embodiment, and without departing from the spirit or essential characteristics of the present invention, energy
It is enough to realize the present invention in other specific forms.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary
, and be nonrestrictive, the scope of the present invention is limited by appended claims rather than described above, it is intended that will fall
All changes in the implication and scope of the equivalency of claim are included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is included
One independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should be by
Specification is as an entirety, and the technical solutions in the various embodiments may also be suitably combined, and forming those skilled in the art can
With the other embodiment of understanding.
Claims (8)
1. a kind of compounded pesticides containing nimbin and double third ring worm esters, it is characterised in that：The compounded pesticides include print
Chinaberry element and double third ring worm esters, wherein, the quality proportioning of nimbin and double third ring worm esters is 1:1~1:100.
2. a kind of compounded pesticides containing nimbin and double third ring worm esters as described in being required right 1, it is characterised in that：Print chinaberry
The quality proportioning of element and double third ring worm esters is 1:1~1:60.
3. a kind of compounded pesticides containing nimbin and double third ring worm esters as described in being required right 2, it is characterised in that：Print chinaberry
The quality proportioning of element and double third ring worm esters is 1:1~1:20.
4. a kind of compounded pesticides containing nimbin and double third ring worm esters as described in being required right 3, it is characterised in that：Print chinaberry
The quality proportioning of element and double third ring worm esters is 1:1~1:10.
5. a kind of compounded pesticides containing nimbin and double third ring worm esters as described in being required right 4, it is characterised in that：Print chinaberry
The quality proportioning of element and double third ring worm esters is 1:5.
6. a kind of application of compounded pesticides containing nimbin and double third ring worm esters as described in right 1-5, it is characterised in that：
The compounded pesticides are used for controlling sucking insects.
7. a kind of application of compounded pesticides containing nimbin and double third ring worm esters as described in right 6, it is characterised in that：Will
Compounded pesticides containing the double third ring worm esters of 1.8%~3.6% nimbin are used for 600~1000 times of preventing and treating thorns of field dilution and inhaled
Formula insect.
8. a kind of application of compounded pesticides containing nimbin and double third ring worm esters as described in right 7, it is characterised in that：Will
Compounded pesticides containing the double third ring worm esters of 3% nimbin are used for field and dilute 600~1000 times of controlling sucking insects.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108402073A (en) * | 2018-05-15 | 2018-08-17 | 信阳农林学院 | It is a kind of prevention rice grub biological pesticide composite and its application |
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| CN101626687A (en) * | 2007-03-08 | 2010-01-13 | 明治制果株式会社 | Pest control composition |
| CN103118537A (en) * | 2010-09-14 | 2013-05-22 | 巴斯夫欧洲公司 | Composition containing a pyripyropene insecticide and a base |
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- 2017-05-27 CN CN201710390129.1A patent/CN107027784A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101626687A (en) * | 2007-03-08 | 2010-01-13 | 明治制果株式会社 | Pest control composition |
| CN103535360A (en) * | 2007-03-08 | 2014-01-29 | 明治制果药业株式会社 | Pest control composition |
| CN103118537A (en) * | 2010-09-14 | 2013-05-22 | 巴斯夫欧洲公司 | Composition containing a pyripyropene insecticide and a base |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108402073A (en) * | 2018-05-15 | 2018-08-17 | 信阳农林学院 | It is a kind of prevention rice grub biological pesticide composite and its application |
| CN108402073B (en) * | 2018-05-15 | 2020-07-10 | 信阳农林学院 | Biopesticide composition for preventing and treating rice pests and application thereof |
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Application publication date: 20170811 |