CN104663621B - A kind of Bradysia odoriphaga gathers cage and its acquisition method - Google Patents
A kind of Bradysia odoriphaga gathers cage and its acquisition method Download PDFInfo
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- CN104663621B CN104663621B CN201510119405.1A CN201510119405A CN104663621B CN 104663621 B CN104663621 B CN 104663621B CN 201510119405 A CN201510119405 A CN 201510119405A CN 104663621 B CN104663621 B CN 104663621B
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Abstract
A kind of Bradysia odoriphaga gathers cage and its acquisition method, and it is related to a kind of Bradysia odoriphaga collection cage and its acquisition method.It includes cage body, gauze nappe, base plate light source, plate, support, cape lid and power supply.The trend that the present invention is creeped upwards using insect, and induce Bradysia odoriphaga using the LED of different colours, enter in cage body from the gauze nappe of 40 mesh of the invention, because other positions of cage body are covered with the gauze of 80 mesh, and into the angle of 90o between gauze nappe and support, therefore, it is difficult to flee from into the Bradysia odoriphaga in cage body, the Bradysia odoriphaga in cage body is drawn into by light source, cage body bottom is extinguished or fallen into due to physical demands in light source.In the culture dish that cage body bottom of the invention prepares, so that Bradysia odoriphaga is laid eggs, screen optimum condition to realize adult seizure, simultaneously can be used for the research of field population pests occurrence rule.
Description
Technical field
The present invention relates to a kind of Bradysia odoriphaga collection cage and its acquisition method.
Background technology
Bradysia odoriphaga Bradysia odoriphaga (Yang et Zhang), larva is referred to as fragrant-flowered garlic maggot, category Diptera,
Nematocera, eye Sciaridae, Bradysia Sciara.Bradysia odoriphaga is the distinctive species of China, and larva is often clustered in leek ground
Damage on lower false stem and bulb, preventing and treating is difficult.Damaging range is wide, and Liliaceae, composite family, Chenopodiaceae, cross can be endangered according to statistics
The various vegetables of 7 section such as Hua Ke, Curcurbitaceae and Umbelliferae 30, wherein northern protecting field leek is aggrieved most heavy, secondly it is garlic, ocean
Green onion, melon and lettuce.According to investigations edible mushroom can be also endangered on Jiangxi, Beijing and other places.It is widely distributed in northern China, northeast, west
North and middle part, are the formidable enemies of leek production.
Chemical pesticide is mainly used for the preventing and treating of Bradysia odoriphaga, such as Le Siben, phoxim, chlorfluazuron, go out at present
Fly amine etc..The residues of pesticides severe overweight in leek is caused also to have been reported that due to improper use or excessive application.Therefore in good time
Monitoring Bradysia odoriphaga field population pests occurrence rule, understands field population resistance occurrence dynamics to farmland specific aim pest control
With great importance.It is main using different colours viscose board and light trap Bradysia odoriphaga, but these sides at present
Method can not but obtain the Bradysia odoriphaga of existence.So far, the technology not caught on Bradysia odoriphaga work worm also
Research.
The content of the invention
The present invention provides a kind of Bradysia odoriphaga collection cage and its collection side to solve above-mentioned problem
Method.
A kind of Bradysia odoriphaga collection cage of the invention, it include cage body, gauze nappe, base plate, light source, plate,
Support, cape lid and power supply；
Described gauze nappe includes gauze nappe one and gauze nappe two；Wherein, gauze nappe one and yarn
Net nappe two is the reverse frustoconic of upper and lower opening, and gauze nappe one uses 40 purposes with gauze nappe two in peripheral part
Gauze is covered, and remainder is covered with 80 mesh gauzes；
Described cage body bottom is base plate, and top is gauze nappe, and wherein base plate leads to the lower end of gauze nappe one
Multiple support connections are crossed, the upper end of gauze nappe one is connected with the lower end of gauze nappe two by multiple supports；
Light source passes through multiple supports and be connected with the upper end of gauze nappe two, covering cape lid on light source, described light source and
Power supply is connected；Wherein, the bottom of gauze nappe one and gauze nappe two is connected angulation and is 90 ° with each support.
A kind of method for gathering Bradysia odoriphaga of the invention, it is followed the steps below：
First, Bradysia odoriphaga collection cage is placed in Bradysia odoriphaga acquisition zone；
2nd, spawning culture medium is added in the plate to Bradysia odoriphaga collection cage, then light source is put into cage body solid
It is fixed, then light source is connected with power supply, by the open and close of power supply light source, finally covered with cape lid in cage body；
Wherein, spawning culture medium is formulated as follows：The agar that weight/mass percentage composition is 2.5% is poured into plate, agar is treated
Tile a sterilizing filter paper thereon after cooling, takes leek, shallot, round onions, garlic, hair green onion, cucumber, radish, Chinese cabbage and potato root
One or more of stem are mixed by any ratio, and are cut into the stem tuber of 0.5 × 1.0 × 1.0cm and are positioned in plate；Described
Light source is the LED lamp of white, blue, green, yellow, powder and red six kinds of colors.
The invention has the advantages that：
The trend that the present invention is creeped upwards using insect, and Bradysia odoriphaga is induced using the LED of different colours,
Enter in cage body from the gauze nappe of 40 mesh of the invention, because other positions of cage body are covered with the gauze of 80 mesh, and
Angle in 90 ° between gauze nappe and support, therefore, it is difficult to flee from into the Bradysia odoriphaga in cage body, by light source
The Bradysia odoriphaga in cage body is drawn into, cage body bottom is extinguished or fallen into due to physical demands in light source.
Prepare 2.5% agar layer in cage body bottom of the invention and pour into 9cm culture dishes, being put into one after agar cooling goes out
Bacterium filter paper, takes leek, shallot, round onions, garlic, hair green onion, cucumber, radish, Chinese cabbage and potato rhizome for test material, so as to leek
Bradysia fungus gnat is laid eggs, and screens optimum condition to realize adult seizure, simultaneously can be used for grinding for field population pests occurrence rule
Study carefully.As understand field population resistance occurrence dynamics and provide theoretical foundation, also for the prevention and control of farmland specific aim insect are provided
Great importance.Meanwhile, the present invention is studied by the light source to different colours, and Bradysia odoriphaga has more apparent trend
In a certain monochromatic trend, there is significant difference compared with other colors to green light.
In addition, cage body structure of the invention, support is demountable structure, after striking, gauze nappe one and two
And light source can be put into the cylindrical structure of cage body bottom, be convenient for carrying.
Brief description of the drawings
Fig. 1 is structural representation of the invention.
Specific embodiment
Specific embodiment one：With reference to figure explanation present embodiment, a kind of Bradysia odoriphaga collection of present embodiment
Cage, it includes cage body 1, gauze nappe 2, base plate 3, light source 4, plate 5, support 6, cape lid 7 and power supply 8；
Described gauze nappe 2 includes the 2-1 of the gauze nappe one and 2-2 of gauze nappe two；Wherein, gauze nappe
One 2-1 and the 2-2 of gauze nappe two are the reverse frustoconic of upper and lower opening, the 2-1 of the gauze nappe one and 2-2 of gauze nappe two
Covered with the gauze of 40 mesh in peripheral part, remainder is covered with 80 mesh gauzes；
The described bottom of cage body 1 is base plate 3, and top is gauze nappe 2, and wherein base plate 3 is with the 2-1's of gauze nappe one
Lower end passes through multiple supports 6 and connects, and the upper end of the 2-1 of gauze nappe one is propped up with the lower end of the 2-2 of gauze nappe two by multiple
Frame 6 is connected；
Light source 4 passes through multiple supports 6 and is connected with the 2-2 upper ends of gauze nappe two, and cape lid 7 is covered on light source 4, described
Light source 4 is connected with power supply 8；Wherein, the bottom of the 2-1 of gauze nappe one and the 2-2 of gauze nappe two is connected institute with each support 6
It is angled to be 90 °.
Specific embodiment two：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：Base plate 3 is the cylindrical structure of upper end open.Other compositions and connected mode are identical with specific embodiment one.
Specific embodiment three：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：Described support 6 is shelf support.Other compositions and connected mode are identical with specific embodiment one.
Specific embodiment four：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：Power supply 8 for can the Push And Release of timing controlled light source 4 device.Other compositions and connected mode and the phase of specific embodiment one
Together.
Specific embodiment five：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：It is the cage body 1 of 1/3rd volumes of covering that cape lid 7 is covered on light source 4.Other compositions and connected mode and specific implementation
Mode one is identical.
Specific embodiment six：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：Light source 4 is illuminated using the LED lamp of white, blue, green, yellow, powder and red six kinds of colors.Other compositions and connected mode
It is identical with specific embodiment one.
Specific embodiment seven：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：The 2-1 of gauze nappe one is the Zhou Changwei 50cm, the Zhou Changwei of bottom surface of the upper bottom surface of the reverse frustoconic of upper and lower opening
40cm, a height of 5cm of round platform.Other compositions and connected mode are identical with specific embodiment one.
Specific embodiment eight：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：The 2-2 of gauze nappe two is the Zhou Changwei 40cm, the Zhou Changwei of bottom surface of the upper bottom surface of the reverse frustoconic of upper and lower opening
30cm, a height of 5cm of round platform.Other compositions and connected mode are identical with specific embodiment one.
Specific embodiment nine：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：The 2-1 of gauze nappe one and the 2-2 of gauze nappe two at intervals of 10cm.Other compositions and connected mode and specific reality
Apply mode one identical.
Specific embodiment ten：Present embodiment, present embodiment and the difference of specific embodiment one are illustrated with reference to figure
It is：The material of the bottom of cage body 1 is resin.Other compositions and connected mode are identical with specific embodiment one.
Specific embodiment 11：With reference to figure explanation present embodiment, a kind of collection leek Bradysia gill fungus of present embodiment
The method of mosquito, it is followed the steps below：
First, the Bradysia odoriphaga collection cage of specific embodiment one is placed in Bradysia odoriphaga acquisition zone；
2nd, spawning culture medium is added in the plate 5 to Bradysia odoriphaga collection cage, then light source 4 is put into cage body 1
It is fixed, then light source 4 is connected with power supply 8, the open and close of light source 4 are controlled by power supply 8, finally use cape in cage body 1
Lid 7 is covered；
Wherein, spawning culture medium is formulated as follows：The agar that weight/mass percentage composition is 2.5% is poured into plate 5, agar is treated
Tile a sterilizing filter paper thereon after cooling, takes leek, shallot, round onions, garlic, hair green onion, cucumber, radish, Chinese cabbage and potato root
One or more of stem are mixed by any ratio, and are cut into the stem tuber of 0.5 × 1.0 × 1.0cm and are positioned in plate 5；
Described light source 4 is the LED lamp of white, blue, green, yellow, powder and red six kinds of colors.
Specific embodiment 12：This city implementation method from unlike specific embodiment 11：Described spawning training
Support base in 0.5 × 1.0 × 1.0cm stem tuber, be put into plate 5 by random district's groups method, arrangement arranged in 3 rows 3, line-spacing and indulge
Away from being 2cm.Other are identical with specific embodiment 11.
Specific embodiment 13：This city implementation method from unlike specific embodiment 11：Described light source 4
It is 12 hours to open with closure interval time.Other are identical with specific embodiment 11.
Beneficial effects of the present invention are verified by following examples：
1st, materials and methods
1.1 for examination insect
Bradysia odoriphaga is provided by the plant protection of Tianjin academy of agricultural sciences, and in laboratory, internal reference is permitted quiet poplar et al.]Report
Raised, temperature is 25 ± 1 DEG C, and the photoperiod is 16h：8h, humidity is 70%r.h.
1.2 for examination host
Shallot (Alliumfistulosum L.), round onions (Allium epa L), garlic (Allium sativum L.),
Hair green onion (Allium fistulosum), cucumber (Cucumis sativus Linn.), radish (Raphanus sativus L),
Chinese cabbage (Brassicapekinensis (Lour.) Rupr.), potato (Solanum tuberosum) is purchased in the organic vegetable in market
Dish shopping area, leek (Allium tuberosum Rottl.ex Spreng.), green bamboo snake kind is from Harbin, Heilongjiang Province
Leek area is planted for many years and is transplanted in optical culture room the plantation carried out in laboratory in Hulan District.
1.3 for examination LED light
The battery of 12V9AH models and supporting charger are purchased from You Tai Science and Technology Ltd.s of Shenzhen, T10W5W models
LED (white, blue, green, yellow, powder is red) is with supporting T10 sockets purchased from the Guangzhou number of attaining Automobile Products firm.
1.4 Bradysia odoriphagas are determined to different hosts spawning taxis
Prepare 2.5% agar layer and pour into 9cm culture dishes, a sterilizing filter paper is put into after cooling, take leek, shallot, round onions,
Garlic, hair green onion, cucumber, radish, Chinese cabbage and potato rhizome are test material, 0.5 × 1.0 × 1.0cm fritters are cut into, by random area
Group method for designing is put into culture dish, and arrangement is arranged in 3 rows 3, and line-spacing and advance are 2cm, are repeated 5 times and are put into diameter 9cm, 15cm high
In insect cage.The adult 10 pairs sprouted wings at the beginning of random acquisition is put into insect cage, and every 2 hours are observed once, in zoom stereoscope
Check 10 hours Bradysia odoriphaga spawning quantity of statistics.
1.5 Bradysia odoriphagas are determined to LED monochromatic lights taxis
The light that Bradysia odoriphaga is inoculated in leek plantation in laboratory is trained indoor, allows it to be bred, form stabilization
Experimental population.Inserted on supporting wire socket using the LED lamp of white, blue, green, yellow, powder and red six kinds of colors, be put into 10cm
In diameter × 10cm Long Circle paper webs, the side seal fixed lamp-socket of paper web one, opposite side seals coating butter with transparent plastic cloth, with
Machine is put in potted plant leek 5cm tops, was turned on light every 12 hours and traps observation once, and continuous monitoring 10 days records result.
2. tested using the Bradysia odoriphaga collection cage of specific embodiment one, it is as a result as follows：
The selectivity that 2.1 Bradysia odoriphagas are laid eggs to different hosts
In addition to round onions, Bradysia odoriphaga can lay eggs (be shown in Table 1) in 4 section, 8 kinds of vegetables rhizome sections, but laying
There is significant difference between amount.Egg laying amount is maximum on plant of Solanaceae potato, and 10h ovum individual amounts reach 93, account for spawning total amount
45.27%, be secondly the radish of cucumber cucurbitaceous and Cruciferae, 10h ovum individual amounts reach respectively 31 and 29,
Account for the 15.27% and 14.09% of spawning total amount.
The Bradysia odoriphaga of table 1 determines table in different hosts spawning taxis
2.2 Bradysia odoriphagas are determined to LED taxis
The Bradysia odoriphaga of table 2 determines table to taxis under LED modules with different colors light
From Bradysia odoriphaga to LED modules with different colors lamp phototaxis research (being shown in Table 2) find, Bradysia odoriphaga have compared with
A certain monochromatic trend is clearly tended to, has significant difference compared with other colors to green light, lure within 12 hours worm amount to reach 27
Head, accounts for and all lures the 40.38% of worm amount；Secondly it is blue and white light, is not intended to pink colour, yellow and red light substantially.
2.3 Bradysia odoriphaga field acquisitions
Laid eggs in different vegetable by this experiment Bradysia odoriphaga and the phototaxis of different colours light can draw, in the wild
During collection adult, the worm living for carrying out Bradysia odoriphaga using green glow is collected, and induces Bradysia odoriphaga using potato
Spawning, so as to obtain a large amount of Bradysia odoriphagas for living.
By above-mentioned experiment, it can be shown that collection cage of the invention can be good at flutterring catches Bradysia odoriphaga, and make research
Person studies the aspects such as its oviposition selectivity, phototaxis well, illustrates that collection cage of the invention can meet Bradysia odoriphaga field
Between population pests occurrence rule investigation, the adult of existence can be also obtained to further appreciate that the resistance occurrence dynamics of the insect population,
For follow-up experimental study provides theoretical foundation and test basis.
Claims (10)
1. a kind of Bradysia odoriphaga gathers cage, it is characterised in that it includes cage body (1), gauze nappe (2), base plate (3), light
Source (4), plate (5), support (6), cape lid (7) and power supply (8)；
Described gauze nappe (2) includes gauze nappe one (2-1) and gauze nappe two (2-2)；Wherein, gauze covering
Body one (2-1) and gauze nappe two (2-2) are the reverse frustoconic of upper and lower opening, and gauze nappe one (2-1) covers with gauze
Lid two (2-2) is covered in peripheral part with the gauze of 40 mesh, and the remainder of cage body is covered with 80 mesh gauzes；
Described cage body (1) bottom is base plate (3), and top is gauze nappe (2), wherein base plate (3) and gauze nappe one
The lower end of (2-1) passes through multiple supports (6) and connects, under the upper end of gauze nappe one (2-1) and gauze nappe two (2-2)
End is connected by multiple supports (6)；
Light source (4) is connected by multiple supports (6) with gauze nappe two (2-2) upper end, and cape lid (7) is covered on light source (4),
Described light source (4) is connected with power supply (8)；Wherein, the bottom of gauze nappe one (2-1) and gauze nappe two (2-2) with
Each support (6) connection angulation is 90 °.
2. a kind of Bradysia odoriphaga according to claim 1 gathers cage, it is characterised in that base plate (3) is upper end open
Cylindrical structure.
3. a kind of Bradysia odoriphaga according to claim 1 gathers cage, it is characterised in that described support (6) is removable
Unload support.
4. a kind of Bradysia odoriphaga collection cage according to claim 1, it is characterised in that power supply (8) is for can timing controlled
The device of light source (4) Push And Release.
5. a kind of Bradysia odoriphaga according to claim 1 gathers cage, it is characterised in that cape lid is covered on light source (4)
(7) it is the cage body (1) of 1/3rd volumes of covering.
6. a kind of Bradysia odoriphaga collection cage according to claim 1, it is characterised in that light source (4) using it is white, blue,
The LED lamp of green, yellow, powder and red six kinds of colors is illuminated.
7. a kind of Bradysia odoriphaga collection cage according to claim 1, it is characterised in that gauze nappe one (2-1) is
The Zhou Changwei 50cm of the upper bottom surface of the reverse frustoconic of upper and lower opening, the Zhou Changwei 40cm of bottom surface, a height of 5cm of round platform.
8. a kind of Bradysia odoriphaga collection cage according to claim 1, it is characterised in that gauze nappe two (2-2) is
The Zhou Changwei 40cm of the upper bottom surface of the reverse frustoconic of upper and lower opening, the Zhou Changwei 30cm of bottom surface, a height of 5cm of round platform.
9. a kind of Bradysia odoriphaga collection cage according to claim 1, it is characterised in that gauze nappe one (2-1) with
Gauze nappe two (2-2) at intervals of 10cm.
10. it is a kind of gather Bradysia odoriphaga method, it is characterised in that it is followed the steps below：
First, the Bradysia odoriphaga collection cage of claim 1 is placed in Bradysia odoriphaga acquisition zone；
2nd, to addition spawning culture medium in the plate (5) of Bradysia odoriphaga collection cage, light source (4) is then put into cage body (1)
Interior fixation, then light source (4) is connected with power supply (8), the open and close of light source (4) are controlled by power supply (8), finally in cage body
(1) covered with cape lid (7) on；
Wherein, spawning culture medium is formulated as follows：The agar that weight/mass percentage composition is 2.5% is poured into plate (5), treats that agar is cold
But tile a sterilizing filter paper thereon after, takes leek, shallot, round onions, garlic, hair green onion, cucumber, radish, Chinese cabbage and potato rhizome
One or more be mixed by any ratio, and be cut into the stem tuber of 0.5 × 1.0 × 1.0cm and be positioned in plate (5)；
Described light source (4) is the LED lamp of white, blue, green, yellow, powder and red six kinds of colors.
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| CN201510119405.1A CN104663621B (en) | 2015-03-18 | 2015-03-18 | A kind of Bradysia odoriphaga gathers cage and its acquisition method |
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| CN201510119405.1A CN104663621B (en) | 2015-03-18 | 2015-03-18 | A kind of Bradysia odoriphaga gathers cage and its acquisition method |
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| CN105145488B (en) * | 2015-07-08 | 2019-09-06 | 山东省农业科学院植物保护研究所 | A method of it traps and monitors bradysia odoriphaga larva |
| CN109221026B (en) * | 2018-11-20 | 2023-09-22 | 山东农业大学 | Device and method for efficiently collecting slow-eye mushroom mosquito eggs of Chinese chives |
| CN112753667B (en) * | 2020-12-31 | 2022-06-17 | 黑龙江大学 | Pineapple-shaped small night lamp type cockroach trap |
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| CN2303460Y (en) * | 1997-08-05 | 1999-01-13 | 郝更生 | Practical energy-saving moth-catching device |
| CN2762542Y (en) * | 2004-01-06 | 2006-03-08 | 周德安 | Insect trap |
| CN2838283Y (en) * | 2005-09-05 | 2006-11-22 | 陈重光 | Funnel cage lamp lures the insect device |
| CN201403438Y (en) * | 2009-05-07 | 2010-02-17 | 胡锡林 | Luminous flytrap with fly-luring bait |
| US20110283599A1 (en) * | 2010-05-23 | 2011-11-24 | Tsung-Hsien Wu | Flytrap |
| ES2367388B1 (en) * | 2011-06-01 | 2012-05-09 | Probodelt S.L. | Insect trap in flying phase, use of said trap and corresponding manufacturing procedure. |
| KR20130022890A (en) * | 2011-08-26 | 2013-03-07 | 김성구 | Insect captor |
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