CN108721425B

CN108721425B - Lung-clearing acupoint application paste and preparation method thereof

Info

Publication number: CN108721425B
Application number: CN201810601133.2A
Authority: CN
Inventors: 陶富文; 曹宇新
Original assignee: Tianjin Zhongke Zhongsheng Medical Technology Co ltd
Current assignee: Tianjin Zhongke Zhongsheng Medical Technology Co ltd
Priority date: 2018-06-12
Filing date: 2018-06-12
Publication date: 2021-06-04
Anticipated expiration: 2038-06-12
Also published as: CN108721425A

Abstract

The invention discloses an acupoint application paste for preventing and treating haze and clearing lung-heat and a preparation method thereof, wherein the preparation method comprises the following steps: (1) decocting herba Houttuyniae, bupleuri radix, Carthami flos, semen Persicae, herba Ephedrae preparata, semen Armeniacae amarum, and cortex Mori in water, and filtering to obtain filtrate and residue; (2) drying the filter residue, and crushing to obtain filter residue powder; mixing the residue powder with the filtrate. The acupoint application paste for preventing and treating haze and clearing away the lung-heat utilizes the traditional Chinese medicine acupoint and five-element therapy, fully exerts the effects of using the paste at any time and any place, has long acting period, no toxic or side effect, safe and reliable effect of drug administration through skin, and has wider practicability and applicability; has the effects of clearing heat, reducing phlegm, tonifying qi, moistening lung, clearing heat, removing toxic substances, purging lung, relieving cough, eliminating phlegm and relieving asthma, and the preparation method is simple.

Description

Lung-clearing acupoint application paste and preparation method thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to an acupoint application paste for preventing and treating haze and clearing away the lung-heat and a preparation method thereof.

Background

In recent years, China has many outbreaks of large-area haze events, and the influence on health is a topic of great public concern. The economic research on health discovers that the average economic loss of health hazards caused by air pollution in Tangshan reaches 414 yuan/person in 2004; the economic loss of health hazard caused by haze pollution in Shanghai city in 2009 is 72.48 billion yuan, which accounts for 0.49% of GDP in Shanghai city in the same year. The health effects of haze are first manifested as an effect on the respiratory system. Research shows that haze entering lung blocks local tissues, so that the ventilation function of local bronchus is reduced, the ventilation function of bronchioles and alveoli is lost, the respiratory defense function is damaged under long-term action, and bronchitis, chronic cough, emphysema, bronchial asthma and the like occur. Although the mechanism of these chronic respiratory diseases is complex, and the medical community has not yet made a clear conclusion about the etiology of the diseases, it is well recognized that air pollution, haze and physique are several important causes.

At present, western medicines for treating bronchitis, chronic cough, emphysema and bronchial asthma mainly comprise antibiotics, central antitussives and hormone medicines, and after long-term use of the medicines, the medicines not only easily generate drug resistance, but also easily cause the immunity of the organism to be reduced, so that the course of disease is prolonged. The traditional Chinese medicine plays an important role in treating chronic diseases such as bronchitis, chronic cough, emphysema and bronchial asthma. The traditional Chinese medicine considers that' the pulse qi flows through, the meridian qi enters the lung, the lung faces all veins, and the essence is transported to the skin and hair. The hair vein is the essence which can move qi in the house. The spirit is kept in the four zang organs, and qi is balanced. The main physiological functions of the lung include governing qi, dispersing and descending, governing respiration, regulating the water passage and directing all the vessels. The lung is associated with skin and hair, so it is vulnerable to attack by exogenous pathogenic factors and is not resistant to cold and heat. The lung body is clear and deficient, and the quality is delicate, so the lung belongs to a delicate organ, and semen is consumed when the lung is too dry; too much diarrhea may cause sweating and yang deficiency. For chronic diseases of respiratory tract such as bronchitis, chronic cough, emphysema and bronchial asthma, the traditional Chinese medicine is classified into the symptoms of cough without phlegm and throat itching caused by lung qi deficiency due to lung qi obstruction, lung injury caused by long-term cough, or cough and asthma due to kidney failure in receiving qi, or lung fluid consumption, qi consumption and yin consumption, and body resistance mutual conflict, and the chronic respiratory diseases are treated by a kidney tonifying, spleen strengthening and lung heat clearing method, a soil reinforcing and vital essence generation method, a wind dispelling and lung moistening method, a qi tonifying and lung nourishing method and the like.

At present, western medicines or traditional Chinese medicines are mostly used for treating acute and chronic bronchitis and chronic cough, although the medicines have quick effect and good curative effect, the medicines have larger toxic and side effects and are inconvenient to use and the like. The practicability and the applicability are wider.

Disclosure of Invention

In order to make up for the defects of the prior art, the invention provides the acupoint application paste for preventing and treating haze and clearing away the lung-heat, which is simple to prepare, convenient to use and obvious in curative effect, and the preparation method thereof.

The technical scheme adopted by the invention for solving the technical problems is as follows:

the invention relates to a preparation method of an acupoint application paste for preventing and treating haze and clearing lung, which comprises the following steps:

(1) decocting herba Houttuyniae, bupleuri radix, Carthami flos, semen Persicae, herba Ephedrae preparata, semen Armeniacae amarum, and cortex Mori in water, and filtering to obtain filtrate and residue;

(2) drying the filter residue, and crushing to obtain filter residue powder; mixing the residue powder with the filtrate.

Further, the preparation method of the acupoint application paste for preventing and treating haze and clearing lung-heat comprises the following steps:

(1) adding 1.5-2.5 parts by weight of houttuynia cordata, 1.5-2.5 parts by weight of radix bupleuri, 1.5-2.5 parts by weight of safflower, 1.5-2.5 parts by weight of peach kernel, 0.5-1.5 parts by weight of honey ephedra, 0.5-1.5 parts by weight of almond and 0.5-1.5 parts by weight of cortex mori into 15-25 parts by weight of water, decocting for 1-2 hours, and filtering to obtain filtrate and filter residue;

The decocting temperature is 85-100 deg.C.

The aperture of the filter cloth for filtering is 200-500 meshes.

The drying temperature is 30-60 ℃ and the drying time is 1-5 days.

The mass ratio of the filter residue powder to the filtrate is 1: (0.1-0.5).

The crushing comprises three steps of pretreatment, coarse crushing and ball milling crushing.

The pretreatment is that the dried filter residue is kept for 2-5min under the steam pressure of 0.5-1MPa and the temperature of 130-; and (3) performing microwave plasma puffing on the steam explosion material for 5-15min at the microwave frequency of 2400-.

And the coarse grinding is to perform coarse grinding on the pretreated filter residue by using a turbine grinder to 20-50 meshes.

And the step of ball milling and crushing is to perform ball milling and crushing on the filter residue after the coarse crushing by using a ball mill to obtain 200-400 meshes.

Further, the ball milling and crushing are carried out by mixing the filter residue after the coarse crushing and grinding aid according to the mass ratio of 1: (0.005-0.015) adding the mixture into a ball mill for ball milling and crushing to 200-400 meshes.

The grinding aid is modified microcrystalline cellulose and/or chitosan oligosaccharide. Further, the grinding aid is prepared by mixing 65-75wt% of modified microcrystalline cellulose and 25-35wt% of chitosan oligosaccharide.

The preparation method of the modified microcrystalline cellulose comprises the following steps: adding 10-30g of microcrystalline cellulose into 95-105g of water, ultrasonically dispersing for 10-30min under the ultrasonic power of 200-400W and the ultrasonic frequency of 30-45kHz, then adding 1-4g of tartaric acid, stirring for 50-100min at the speed of 400-800r/min, then centrifugally separating for 15-25min at the rotation speed of 5000-8000r/min, removing the supernatant, and drying the precipitate at the temperature of 40-60 ℃ to constant weight to obtain the modified microcrystalline cellulose.

An acupoint application paste for preventing and treating haze and clearing lung-heat is prepared by the preparation method of the acupoint application paste for preventing and treating haze and clearing lung-heat.

The invention adopts the raw materials with the functions and pharmacological researches:

houttuynia cordata: clearing away heat and toxic material, relieving swelling, treating sore, promoting urination, removing dampness, clearing away heat, relieving dysentery, invigorating stomach, resolving food stagnation, and resisting virus. The product is dried aerial part of Houttuynia cordata Thunb of Saururaceae. Harvesting in summer when stem and leaf are flourishing and flower ear is more, removing impurities, and drying in the sun.

Bupleurum root: to expel pathogenic factors from the exterior, release heat, soothe the liver, relieve depression and lift yang qi. The product is dried root of Bupleurum chinense DC or Bupleurum scorzonerifolium Willd. According to the different characteristics, it is called "Bei chai Hu" and "nan chai Hu" respectively. Collected in spring and autumn, removed stems and leaves and silt, and dried.

Safflower: promoting blood circulation, dredging channels, removing blood stasis, and relieving pain. It is also called as the upper part and stigma of crocus sativus L of Iridaceae. And (4) harvesting flowers in the morning of sunny days of 9-10 months, picking down stigmas, and drying to obtain the flower buds. And (5) placing the mixture in a cool and dry place, and sealing and storing the mixture.

Peach kernel: with symptoms of lumps, pulmonary abscess, intestinal abscess, traumatic injury and pain. The product is dried mature seed of Prunus persica (L.) Batsch or Prunus davidiana (Carr.) Franch of Rosaceae. Harvesting after fruit ripening, removing pulp and nucleocapsid, taking out seed, and drying in the sun.

Honey ephedra: induce sweating and dispel cold, ventilate lung and relieve dyspnea, induce diuresis to alleviate edema. The herba Ephedrae is dried grass stem of Ephedra sinica Sinica Stapf, Ephedra intermedia Schrenk et C.A.Mey, or Ephedra equiseti equivalent Bge. In autumn, green grass stems are harvested and dried in the sun. The processing method of the honey ephedra comprises the following steps: the honey ephedra is prepared from ephedra segments and fried until the honey ephedra segments are not sticky according to a honey moxibustion method (appendix II D) of the 2010 version of Chinese pharmacopoeia, and the consumption of refined honey is 20 percent.

Almond: direct qi downward to relieve cough and dyspnea, moisten intestines to relieve constipation. The product is bitter dried seed of Prunus Armeniaca or Prunus Armeniaca of Rosaceae. Picking in summer when fruit is mature, removing pulp and core shell, taking out kernel, and air drying. Placing in shady and dry place for preventing worm damage.

White mulberry root-bark: purge lung, relieve dyspnea, induce diuresis to alleviate edema. The product is dry root bark of Morus alba L. Digging root part from late autumn leaf to spring before germination, scraping yellow brown coarse skin, longitudinally cutting, peeling root skin, and sun drying.

The application method and the dosage are as follows: the acupoint plaster is applied to the Lieque acupoint, Zhaohai acupoint and Tiantu acupoint at a dose of 5-40g each time, once a day, 5-40g each time, and ten days as a treatment course.

The acupoint application paste for preventing and treating haze and clearing away the lung-heat utilizes the traditional Chinese medicine acupoint and five-element therapy, fully exerts the effects of using the paste at any time and any place, has long acting period, no toxic or side effect, safe and reliable effect of drug administration through skin, and has wider practicability and applicability; has the effects of clearing heat, reducing phlegm, tonifying qi, moistening lung, clearing heat, removing toxic substances, purging lung, relieving cough, eliminating phlegm and relieving asthma, and the preparation method is simple.

Detailed Description

Introduction of raw materials and equipment in the examples:

the turbine crusher is an Asahi turbine crusher provided by Asahi mechanical Equipment Co., Ltd of Guangzhou, model number SWLF-600, and the rotation speed of the main shaft is 2500 r/min.

Tartaric acid, CAS number: 526-83-0.

The planetary ball mill is an PMQ0.4L type planetary ball mill provided by the company Limited in the Uigo instruments and equipment (Shanghai), the rotating speed of the ball mill is 300r/min, the material of a ball milling tank is stainless steel, the volume of the ball milling tank is 50mL, the using amount of grinding balls is 4, the material of the grinding balls is zirconia, and the specification of the grinding balls is 6 mm.

The microwave source used for microwave plasma puffing is a microwave source provided by vinblastic mitable instruments ltd and having a model number ZWGL-1500 WD.

The microcrystalline cellulose is provided by Huaxing bioengineering Co., Ltd in the Hunan century, the model is 36, and the grade is food grade.

Chitosan oligosaccharide is provided by the biological science and technology limited of Jinan Luyuan, and the grade is food grade.

Steam explosion treatment is called steam explosion for short, and is applied to wood treatment initially, and in the steam explosion process, water vapor containing a large amount of heat energy can be heated to a high temperature instantly, and the material structures of the water vapor and the water vapor can be changed after the water vapor is subjected to instant pressure release.

Microwave puffing is that microwave energy reaches the deep layer of the material and is converted into heat energy, so that the deep water of the material is quickly evaporated to form a higher internal steam pressure condition, and the material is forced to be puffed, thereby being more beneficial to processing and crushing. The microwave puffing method can preserve the original nutrient components in the traditional Chinese medicinal materials of the food to the maximum extent.

Example 1

The preparation method of the acupoint application paste for preventing and treating haze and clearing away the lung-heat comprises the following steps:

(1) adding 2 parts by weight of houttuynia cordata, 2 parts by weight of radix bupleuri, 2 parts by weight of safflower, 2 parts by weight of peach kernel, 1 part by weight of honey ephedra, 1 part by weight of almond and 1 part by weight of white mulberry root bark into 20 parts by weight of water, decocting for 1.5 hours, and filtering to obtain filtrate and filter residue;

(2) drying the filter residue in the sun, and crushing to 200 meshes to obtain filter residue powder; mixing the filter residue powder and the filtrate to prepare a paste, thus obtaining the acupoint application paste for preventing and treating haze and clearing away the lung-heat.

Example 2

(1) adding 2 parts by weight of houttuynia cordata, 2 parts by weight of radix bupleuri, 2 parts by weight of safflower, 2 parts by weight of peach kernel, 1 part by weight of honey ephedra, 1 part by weight of almond and 1 part by weight of white mulberry root bark into 20 parts by weight of water, decocting for 1.5 hours at the temperature of 95 ℃, and filtering by adopting 300-mesh filter cloth to obtain filtrate and filter residue;

(2) drying the filter residue at 45 deg.C for 2d, and pulverizing to obtain filter residue powder; mixing the filter residue powder and the filtrate according to a mass ratio of 1: 0.2, and evenly mixing to obtain the acupoint application paste for preventing and treating haze and clearing away the lung-heat.

The crushing in the step (2) comprises three steps of pretreatment, coarse crushing and ball milling crushing.

The pretreatment comprises the steps of keeping the dried filter residue for 3min under the steam pressure of 0.8MPa and the temperature of 150 ℃, and then instantly releasing pressure to obtain a steam explosion material; and (3) performing microwave plasma puffing on the steam explosion material for 8min under the conditions that the microwave frequency is 2450MHz and the microwave power is 700W.

And the coarse crushing is to perform coarse crushing on the filter residue after the pretreatment to 40 meshes by using a turbine crusher.

And the ball milling and crushing step is that the filter residue after the coarse crushing and a grinding aid are mixed according to the mass ratio of 1: 0.01 is added into a ball mill to be ball-milled to 300 meshes.

The grinding aid is microcrystalline cellulose.

Example 3

The grinding aid is modified microcrystalline cellulose.

The preparation method of the modified microcrystalline cellulose comprises the following steps: adding 20g of microcrystalline cellulose into 100g of water, ultrasonically dispersing for 20min under the ultrasonic power of 300W and the ultrasonic frequency of 40kHz, then adding 2.5g of tartaric acid, stirring for 70min at the speed of 600r/min, then centrifugally separating for 20min at the rotating speed of 6000r/min, removing supernatant, and drying the precipitate at the temperature of 50 ℃ to constant weight to obtain the modified microcrystalline cellulose.

Example 4

The pretreatment is to instantly release pressure after the dried filter residue is kept for 3min under the steam pressure of 0.8MPa and the temperature of 150 ℃.

The grinding aid is modified microcrystalline cellulose.

Example 5

The pretreatment comprises the step of performing microwave plasma puffing on the dried filter residue for 8min under the conditions that the microwave frequency is 2450MHz and the microwave power is 700W.

The grinding aid is modified microcrystalline cellulose.

Example 6

The grinding aid is chitosan oligosaccharide.

Example 7

The grinding aid is prepared by mixing 70 wt% of modified microcrystalline cellulose and 30 wt% of chitosan oligosaccharide.

The DPPH free radical clearance rate of the acupoint application pastes for preventing and treating haze and clearing lung-heat prepared in examples 2-7 was measured.

Preparing a solution to be detected: adding 100mL of deionized water into 10g of the acupoint application paste for preventing haze and clearing away the lung-heat, performing ultrasonic treatment for 1h under the ultrasonic power of 300W and the ultrasonic frequency of 40kHz, and filtering by using 600-mesh filter cloth to obtain a liquid to be detected.

Determination of the DPPH radical clearance: the DPPH method is adopted. DPPH is accurately weighed to prepare a solution with the concentration of 0.2mmol/LDPPH, and the solution is stored in a brown bottle in a dark place at low temperature. And diluting the solution to be detected to 100mg/mL by using deionized water to obtain a sample solution. Taking 2mL and 100mg/mL sample liquid in a test tube, adding 2mL and 0.2mmol/LDPPH solution, mixing uniformly, standing in the dark at room temperature for 30min, measuring the absorbance value at 517nm by using an ultraviolet spectrophotometer UV-2000 (Enicoco (Shanghai) Limited instruments company) to obtain Ai, simultaneously measuring the absorbance value at 517nm of 2mL and 100mg/mL sample liquid by adding 2mL absolute ethyl alcohol, standing in the dark at room temperature for 30min, measuring the absorbance value at 517nm by using an ultraviolet spectrophotometer UV-2000 (Enicoco (Shanghai) Limited instruments company) to obtain Aj, and standing in the dark at room temperature to obtain UV-2000 (Enicoco (Shanghai) Limited instruments company) to obtain Ac. The DPPH radical clearance rate of the sample is calculated according to the following formula: the clearance is [1- (Ai-Aj)/Ac ] × 100%.

The test results are shown in Table 1.

Table 1: DPPH free radical clearance test data table

Clinical observation test

The curative effect of the acupoint application paste for preventing and treating haze and clearing lung-heat is further illustrated by clinical observation tests.

Selecting 300 clinical patients in a hospital in haze days in winter, wherein the patients are 35-50 years old and 43.6 years old in average age, randomly dividing the patients into 6 groups, and using the acupoint application pastes for preventing and treating haze and clearing lung-heat of the embodiments 2-7 respectively for 50 patients in each group, wherein the symptoms are as follows: cough and expectoration with chest pain or dyspnea, and the difference between the basic data of the disease condition, disease course and age has no statistical significance (P is more than 0.05), and is comparable.

The acupoint application pastes the haze-preventing and lung-clearing acupoint application pastes on the Leluokou acupoint, the Zhaohai acupoint and the Tiantu acupoint respectively once a day, the dosage of the haze-preventing and lung-clearing acupoint application paste for each acupoint is 10g, ten days is one treatment course, and the result statistics is carried out after two treatment courses.

The curative effect standard is as follows:

(1) and (3) healing: cough, expectoration, chest pain or dyspnea symptom, and complete disappearance of physical signs;

(2) the method has the following advantages: cough, expectoration, chest pain or dyspnea symptom are obviously relieved, and the physical sign is obviously improved;

(3) and (4) invalidation: there was no significant improvement, or exacerbation, of symptoms before and after treatment.

The difference in therapeutic effect between the groups was statistically significant (P < 0.05). The specific test results are shown in table 2.

Table 2: curative effect test result table

Conclusions were drawn from the above treatments: the acupoint application paste for preventing and treating haze and clearing lung-heat has an obvious treatment effect on patients with lung discomfort in haze days, and has an obvious effect of preventing and treating haze and clearing lung-heat.

Specific examples of the applications

When a woman in the age of 48 years old or in winter suffers from cough and chest distress due to haze, the acupoint application pastes for preventing and treating haze and clearing away the lung-heat are respectively pasted on the Lepidou acupoint, the Zhaohai acupoint and the Tiantu acupoint once a day, the dosage of the acupoint application paste for preventing and treating haze and clearing the lung-heat at each acupoint is 10g, ten days are a treatment course, and after 2 treatment courses of the acupoint application paste for preventing and treating haze and clearing the lung-heat, the acupoint application paste is recovered to be normal and symptoms disappear.

Claims

1. The preparation method of the lung clearing acupoint application paste is characterized by comprising the following steps:

(2) drying the filter residue, and crushing to obtain filter residue powder; mixing the residue powder with the filtrate;

the crushing comprises three steps of pretreatment, coarse crushing and ball milling crushing; the pretreatment is that the dried filter residue is kept for 2-5min under the steam pressure of 0.5-1MPa and the temperature of 130-; performing microwave plasma puffing on the steam explosion material for 5-15min at the microwave frequency of 2400-; and the ball milling and crushing step is that the filter residue after the coarse crushing and a grinding aid are mixed according to the mass ratio of 1: (0.005-0.015) adding the mixture into a ball mill for ball milling and crushing to 200-400 meshes;

the grinding aid is prepared by mixing 65-75wt% of modified microcrystalline cellulose and 25-35wt% of chitosan oligosaccharide.

2. The method for preparing lung-clearing acupoint application paste according to claim 1, wherein the decoction temperature is 85-100 deg.C; the aperture of the filter cloth for filtering is 200-500 meshes; the drying temperature is 30-60 ℃, the drying time is 1-5 days, and the mass ratio of the filter residue powder to the filtrate is 1: (0.1-0.5).

3. The method for preparing lung-clearing acupoint application paste according to claim 1, wherein the coarse pulverization is performed by coarsely pulverizing the pre-treated filter residue to 20-50 mesh by using a turbine pulverizer.

4. The method for preparing the lung-clearing acupoint application paste according to claim 1, wherein the modified microcrystalline cellulose is prepared by the following steps: adding 10-30g of microcrystalline cellulose into 95-105g of water, ultrasonically dispersing for 10-30min under the ultrasonic power of 200-400W and the ultrasonic frequency of 30-45kHz, then adding 1-4g of tartaric acid, stirring for 50-100min at the speed of 400-800r/min, then centrifugally separating for 15-25min at the rotation speed of 5000-8000r/min, removing the supernatant, and drying the precipitate at the temperature of 40-60 ℃ to constant weight to obtain the modified microcrystalline cellulose.

5. An acupoint application paste for clearing away the lung-heat, which is characterized by being prepared by the preparation method of the acupoint application paste for clearing away the lung-heat according to any one of claims 1 to 4.