CN108707604B - 表皮干细胞中采用CRISPR-Cas系统进行CNE10基因敲除 - Google Patents
表皮干细胞中采用CRISPR-Cas系统进行CNE10基因敲除 Download PDFInfo
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- CN108707604B CN108707604B CN201810542016.3A CN201810542016A CN108707604B CN 108707604 B CN108707604 B CN 108707604B CN 201810542016 A CN201810542016 A CN 201810542016A CN 108707604 B CN108707604 B CN 108707604B
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
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CN201810542016.3A CN108707604B (zh) | 2018-05-30 | 2018-05-30 | 表皮干细胞中采用CRISPR-Cas系统进行CNE10基因敲除 |
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CN201810542016.3A CN108707604B (zh) | 2018-05-30 | 2018-05-30 | 表皮干细胞中采用CRISPR-Cas系统进行CNE10基因敲除 |
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CN108707604A CN108707604A (zh) | 2018-10-26 |
CN108707604B true CN108707604B (zh) | 2019-07-23 |
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CN201810542016.3A Active CN108707604B (zh) | 2018-05-30 | 2018-05-30 | 表皮干细胞中采用CRISPR-Cas系统进行CNE10基因敲除 |
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Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
IL294014B1 (en) | 2015-10-23 | 2024-03-01 | Harvard College | Nucleobase editors and their uses |
AU2017306676B2 (en) | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
CN110214180A (zh) | 2016-10-14 | 2019-09-06 | 哈佛大学的校长及成员们 | 核碱基编辑器的aav递送 |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
KR20190130613A (ko) | 2017-03-23 | 2019-11-22 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
CN108715850B (zh) * | 2018-06-05 | 2020-10-23 | 艾一生命科技(广东)有限公司 | 表皮干细胞中采用CRISPR-Cas系统进行GING2基因敲除 |
EP3942040A1 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
MX2022014008A (es) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo. |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107619829B (zh) * | 2017-10-14 | 2018-08-24 | 南京平港生物技术有限公司 | 使用crispr-cas系统对间充质干细胞进行gins2基因敲除的方法 |
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Inventor after: Han Zhibo Inventor after: Yang Jun Inventor after: Zhu Chengguang Inventor before: Yang Jun Inventor before: Zhu Chengguang |
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Effective date of registration: 20190619 Address after: 334000 Binjiang area, Shangrao economic and Technological Development Zone, Jiangxi Applicant after: Jiangxi Han's joint stem cell technology Co., Ltd. Address before: 471000 Luoyang economic and Technological Development Zone, Henan Province, south of Qianmen Street, south of Qianjiang street, 1 units 1-1404 of Yuejiang Road, 1 buildings. Applicant before: Luoyang Xuan Zhi Biological Technology Co., Ltd. |
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