CN108686116A - 复方甘桑抑菌洗液及其制备方法 - Google Patents

复方甘桑抑菌洗液及其制备方法 Download PDF

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CN108686116A
CN108686116A CN201810633088.9A CN201810633088A CN108686116A CN 108686116 A CN108686116 A CN 108686116A CN 201810633088 A CN201810633088 A CN 201810633088A CN 108686116 A CN108686116 A CN 108686116A
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侯小涛
邓家刚
郝二伟
杜正彩
李聪
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Guangxi University of Chinese Medicine
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Abstract

本发明公开了一种复方甘桑抑菌洗液，主要由甘蔗叶、桑白皮、柚叶、八角茴香叶、救必应、毛两面针和薄荷油制成。据此，发明人还建立了相应产品的制备方法。实验证明，本发明的复方甘桑抑菌洗液对大肠埃希菌、金黄色葡萄球菌、铜绿假单胞杆菌、乙型溶血性链球菌、白色念珠菌等其明显抑制作用。因此，可以预见，本发明在治疗由于多种细菌引起的阴道炎的药物方面有较大潜力。

Description

复方甘桑抑菌洗液及其制备方法
技术领域
本发明属于阴道炎治疗技术领域，尤其涉及一种复方甘桑抑菌洗液及其制备方法。
背景技术
阴道炎为病原体入侵阴道，使阴道粘膜产生炎症，白带出现量、色、质的异常。阴道炎尤以细菌性阴道炎居多。目前，西医治疗阴道炎药物多以抗生素和激素类为主，若长期使用，容易产生耐药性，治疗效果不佳；若西医西药频繁使用，会改变妇女阴道环境，造成复发率高，副作用大等问题。
发明内容
本发明要解决的技术问题是提供一种疗效可靠的复方甘桑抑菌洗液及其制备方法。
为解决上述技术问题，本发明采用以下技术方案：
复方甘桑抑菌洗液，主要由甘蔗叶、桑白皮、柚叶、八角茴香叶、救必应、毛两面针和薄荷油制成。
上述复方甘桑抑菌洗液，主要由甘蔗叶750g、桑白皮300g、柚叶450g、八角茴香叶450g、救必应450g、毛两面针750g和薄荷油10g制成。
上述复方甘桑抑菌洗液的制备方法，按以下步骤操作进行：
<1>原材料的净制、炮制、干燥；
<2>提取与浓缩
将净制后的八角茴香叶、柚叶、救必应置提取罐内，加入6倍量的水，提取挥发油，另存；药渣加入甘蔗叶、桑白皮、毛两面针，回流提取两次，每次加入8倍药材量的水，每次1h，合并提取液，过滤，浓缩成稠膏状，取出置干燥洁净的容器内备用；
<3>离心：将步骤<2>所得稠膏置离心机中离心除去不溶性及其他大分子的杂质；
<4>配液：将步骤<3>离心后的稠膏加入配制罐中，加入步骤<2>所得挥发油，再加入纯化水至5L，滤过，制成洗液；
<5>混合：在步骤<4>洗液中加入薄荷油搅拌混合均匀，即得
针对目前阴道炎治疗药物存在的问题，发明人研发了一种复方甘桑抑菌洗液，主要由甘蔗叶、桑白皮、柚叶、八角茴香叶、救必应、毛两面针和薄荷油制成。据此，发明人还建立了相应产品的制备方法。实验证明，本发明的复方甘桑抑菌洗液对大肠埃希菌、金黄色葡萄球菌、铜绿假单胞杆菌、乙型溶血性链球菌、白色念珠菌等其明显抑制作用。因此，可以预见，本发明在治疗由于多种细菌引起的阴道炎的药物方面有较大潜力。
附图说明
图1是本发明复方甘桑抑菌洗液的制备方法工艺流程图。
具体实施方式
一、配方
甘蔗叶750g、桑白皮300g、柚叶450g、八角茴香叶450g、救必应450g、毛两面针750g、薄荷油10g。
二、生产工艺(图1)
1、原材料的净制、炮制、干燥
1.1甘蔗叶：除去杂质，用饮用水冲冼干净，稍润，切段；收集在托盘中，置烘房内于60℃下干燥至含水量为8～12％之间；取出，装入洁净的容器中备用。
1.2桑白皮：除去杂质，收集在托盘中，置烘房内于60℃下干燥至含水量为8～12％之间；取出，装入洁净的容器中备用。
1.3柚叶：除去杂质，用饮用水冲冼干净，稍润；取出，装入洁净的容器中备用。
1.4八角茴香叶：除去杂质，用饮用水冲冼干净，稍润；取出，装入洁净的容器中备用。
1.5救必应：除去杂质，用饮用水冲冼干净，稍润，切段；收集在托盘中，置烘房内于60℃下干燥至含水量为8～12％之间；取出，装入洁净的容器中备用。
1.6毛两面针：除去杂质，用饮用水冲冼干净，稍润，切段；收集在托盘中，置烘房内于60℃下干燥至含水量为8～12％之间；取出，装入洁净的容器中备用。
2、提取与浓缩
按多功能提取罐操作规程进行操作：将净制后的八角茴香叶、柚叶、救必应置提取罐内，加入6倍量的水，提取挥发油，另存；药渣加入甘蔗叶、桑白皮、毛两面针，回流提取两次，每次加入8倍药材量的水，每次1h(煮沸开始计时)，合并提取液，过滤，浓缩成稠膏状(相对密度不低于1.10)；取出置干燥洁净的容器内备用，中检。
3、离心：将稠膏置离心机中离心除去不溶性及其他大分子的杂质，取出置洁净容器内备用。
4、配液：将离心后的稠膏加入配制罐中，加入前述挥发油，再加入纯化水至5L，滤过，制得含生药量为0.62g/ml洗液。
5、混合：加入薄荷油搅拌混合均匀，检验。
6、灌装：将合格的药液泵入高位罐中，通过全自动液体灌装机进行灌装。
7、外包装：对灌装好的药液的瓶子进行贴标，附装说明书，装盒，封箱，打包。
表1本发明复方甘桑抑菌洗液的制备方法所用生产设备目录
三、抑菌实验
1方法
琼脂二倍稀释法。
2材料
菌株：大肠埃希菌(CMCC44102)，金黄色葡萄球菌(CMCC26003)，铜绿假单胞杆菌(ATCC27853)，乙型溶血性链球菌(CMCC32210)，白色念珠菌(ATCC10231)，前四株菌种来自广西中医药大学微生物与免疫教研室保存标准菌株，后一种为购自广东微生物菌种保存中心。
材料：琼脂培养基(北京陆桥，批号：15116)，沙氏培养基(环凯生物，批号：3105452)羊血全血(鸿泉生物，批号：170401)麦氏比浊管，细菌接种环。
3实验步骤
取培养好的四种细菌及白色念珠菌，从培养皿中用接种环挑取少量于装有约2ml无菌生理盐水的试管中，用0.5麦氏比浊管进行比浊。取前述制得复方甘桑抑菌洗液按照对倍稀释法稀释成十个不同浓度的药液，在无菌操作下与趁热与琼脂培养基混合均匀后马上倒入培养平板中，等待冷却至室温后即制成含药培养基。取浊度一致的五种病菌接种于含药琼脂培养基中，四种细菌置于37℃培养箱中培养24h，白色念珠菌置于27℃培养箱中培养7d，以无菌种生长的浓度为该菌的最小抑菌浓度。
4实验结果
表2复方甘桑抑菌洗液抑菌实验结果
5结果分析
如表2所示，本发明的复方甘桑洗液对常见致病菌金黄色葡萄球菌、乙型溶血性链球菌、铜绿假单胞杆菌、大肠埃希菌、白色念珠菌均具有一定的抑菌作用，其中对金黄色葡萄球菌的抑制作用最佳，对铜绿假单胞杆菌的抑制作用次之。

Claims (3)

1.一种复方甘桑抑菌洗液，其特征在于主要由甘蔗叶、桑白皮、柚叶、八角茴香叶、救必应、毛两面针和薄荷油制成。
2.根据权利要求1所述的复方甘桑抑菌洗液，其特征在于主要由甘蔗叶750g、桑白皮300g、柚叶450g、八角茴香叶450g、救必应450g、毛两面针750g和薄荷油10g制成。
3.权利要求2所述的复方甘桑抑菌洗液的制备方法，其特征在于按以下步骤操作进行：
<1>原材料的净制、炮制、干燥；
<2>提取与浓缩
将净制后的八角茴香叶、柚叶、救必应置提取罐内，加入6倍量的水，提取挥发油，另存；药渣加入甘蔗叶、桑白皮、毛两面针，回流提取两次，每次加入8倍药材量的水，每次1h，合并提取液，过滤，浓缩成稠膏状，取出置干燥洁净的容器内备用；
<3>离心：将步骤<2>所得稠膏置离心机中离心除去不溶性及其他大分子的杂质；
<4>配液：将步骤<3>离心后的稠膏加入配制罐中，加入步骤<2>所得挥发油，再加入纯化水至5L，滤过，制成洗液；
<5>混合：在步骤<4>洗液中加入薄荷油搅拌混合均匀，即得。
CN201810633088.9A 2018-06-19 2018-06-19 复方甘桑抑菌洗液及其制备方法 Pending CN108686116A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114246912A (zh) * 2020-09-25 2022-03-29 广西中医药大学 甘蔗叶和八角茴香叶止痒组合物及其制备方法

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