CN108668831A - A kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting - Google Patents
A kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/005—Precision agriculture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/40—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/40—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure
- A01G24/44—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure in block, mat or sheet form
- A01G24/46—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure in block, mat or sheet form multi-layered
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention belongs to plant root-knot nematode inoculation method technical fields, specifically provide a kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting, including：（1）No sick soil acquisition and processing；（2）Sick soil acquires and processing；（3）Sick soil expands numerous culture；（4）Expand numerous sick soil pretreatment；（5）3 layers of sick soil inoculation.The present invention uses 3 layers of sick soil inoculation technique：Upper and lower 2 layers connect no sick soil, middle level wiring parasitosis soil.Compared with existing root-knot nematode egg and larva inoculation technique, for this technology close to being inoculated with naturally, success ratio of inoculation is high, meanwhile, it eliminates and chooses worm link, workload mitigates significantly.Compared with the single sick soil inoculation technique of existing root-knot nematode, this technology sick soil dosage reduces 1/2 2/3, greatly reduce sick soil acquire, transport, expand it is numerous culture etc. workload and cost.And the seedling that energy effective protection has just been transplanted not only can guarantee and be inoculated with successfully, but also be avoided that seedling dies of illness too early not by nematode infection.
Description
Technical field
The invention belongs to plant root-knot nematode inoculation method technical fields, specifically provide a kind of plant root-knot nematode basin
Plant 3 layers of sick soil inocalation method.
Background technology
Plant root-knot nematodes are the soil-borne diseases of plant.In recent years, with the contiguous plant of susceptible host, the tune of sick seedling
Fortune, the expansion of Greenhouse area and multiple crop index increase, and root knot nematode disease generally occurs, aggravates year by year, typically results in and subtract
10%-15% is produced, up to 50% or more, or even total crop failure when serious, becomes the important disease in production.
In root knot nematode disease study on prevention, root knot nematode disease biological control, chemical prevention, Resistance Identification, medicine are carried out
The pot experiments such as agent screening, and can these pot experiments be smoothed out depending on whether root-knot nematode inoculation succeeds.Currently, root
Tie lines worm inoculation technique mainly has ovum inoculation, the inoculation of 2 instar larvaes and single sick soil to be inoculated with 3 kinds of technologies.Wherein, ovum inoculation technique is
By acquiring root knot old complaint, the nematode egg capsule under optical body visor in picking root knot is put into clear water, is made into centainly with clear water
The ovum liquid of ratio, is inoculated into potted plant root；2 instar larvae inoculation techniques be it is above-mentioned choose ovum on the basis of, egg capsule is put into
It being stood in clear water after a certain period of time, 2 instar larvaes that picking is hatched are put into clear water, and a certain proportion of larva liquid is made into clear water,
It is inoculated into potted plant root.This 2 kinds of technologies can be inoculated with accurate quantitative analysis, but success ratio of inoculation is low, chooses the heavy workload of worm, takes
When expenses of labour；Single sick soil inoculation technique is directly transplanted seedling and is inoculated with being fitted into flowerpot after root-knot nematode sick soil mixing.
The inoculation technique close to being inoculated with naturally, and success ratio of inoculation is high, eliminates and chooses worm link, and workload mitigates significantly, saving of work and time, because
This technology extensive use in root knot nematode disease pot experiment.But the existing single sick soil inoculation technique of root-knot nematode also have with
Lower problem needs to solve：Inoculation sick soil dosage is big, and the Potted orchard morbidity of inoculation is early, and some experiments are not completed also, and seedling has not been died of illness.
Invention content
The purpose of the present invention is overcoming drawbacks described above, a kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting are provided, are being protected
On the basis of demonstrate,proving root-knot nematode inoculation successfully, the inoculation dosage of nematode sick soil is reduced, prevents inoculation seedling from dying of illness too early.
Technical scheme of the present invention：A kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting, include the following steps：
（1）No sick soil acquisition and processing
Acquisition pool mud soil, wasteland soil or plant root have no the native without root-knot nematode of root knot symptom, and decomposed excrement is added after crushing
Fertilizer or the organic fertilizers such as agricultural wastes are as obtain no sick soil after base manure, with no root-knot nematode soil mixing spare, organic fertilizer and nothing
The volumetric usage ratio of root-knot nematode soil is 1：10.
（2）Sick soil acquires and processing
There are the old complaint and rhizosphere sick soil of root knot symptom in herborization root, and old complaint is cut in broken addition rhizosphere sick soil and is uniformly mixed so as to obtain disease
Soil is spare；If plant root has the old complaint of root knot symptom to rot, directly it is spare to be uniformly mixed so as to obtain sick soil for acquisition rhizosphere sick soil.
（3）Sick soil expands numerous culture
A, nursery：Commercially available special seedling medium is fitted into 50 hole seedling-growing containers, the seed of the susceptible host of root-knot nematode is sown into and is educated
In seedling matrix, per 1, nursery hole seed, transplanted when seedling grows to 3-5 true leaves；
B, expand numerous culture：Step（2）The spare sick soil of acquisition mixing is divided in flowerpot, and the step A transplantation of seedlings cultivated is existed
On sick soil, per 1-3 plants of basin, after seedling grows to 30-60 days, nematode breeds 1-2 generations, and the root-knot nematode in soil obtains expanding numerous, insect population
Density increases.
（4）Expand numerous sick soil pretreatment
Potting host's overground part cauline leaf that step B is expanded to numerous culture is cut, and root and sick soil are poured out, and will have the old complaint of root knot to cut broken
It is spare that pretreatment sick soil is uniformly mixed so as to obtain in addition sick soil.
（5）3 layers of sick soil inoculation
First add one layer of step in inoculation flowerpot bottom（1）Processed no sick soil, the second layer add step（4）Processed pretreatment
Sick soil topmost adds one layer of step again（1）Processed no sick soil.Transplanting inoculation is directly carried out after installing basin, host 3-5 is true
Leaf age seedling replanting is on upper layer without in sick soil.
Further, in step A, the susceptible host of root-knot nematode is tomato, muskmelon or cucumber.
Further, step（4）In, before expanding numerous sick soil pretreatment, the potting for expanding numerous culture shifts to an earlier date stopping leaching in 4-7 days
Water.
Further, step（5）In, 3 layer soil bodies accumulate amount ratio：Without sick soil：Pre-process sick soil：No sick soil is 1：1：1.
Further, step（5）In, the inoculation flowerpot inner wall is equipped with graduation mark.Convenient for dress soil operation.
Further, flowerpot is divided into 3 layers of the identical upper, middle and lower of volume by the graduation mark.By above-mentioned 3 layers native amount ratio
Flowerpot is divided into 3 layers of upper, middle and lower by example with graduation mark, convenient for by usage ratio layering dress soil.
Advantage of the present invention：1, compared with existing root-knot nematode egg and larva inoculation technique, success ratio of inoculation of the present invention is high,
Incidence is inoculated with up to 100%, sick grade of falling ill meets the requirement of root-knot nematode pot experiment at 3-5 grades.And it eliminates and chooses worm link, work
It measures and mitigates significantly.
2, compared with the single sick soil inoculation technique of existing root-knot nematode, sick soil provided by the invention expands numerous culture technique,
Root-knot nematode in collected sick soil can be made to obtain expanding numerous, sick soil insect density increase, worm sources guarantee is successfully provided to be inoculated with.
3, the present invention use 3 layers of sick soil inoculation technique, per potting soil up and down 2 layers for no nematode soil, only among one layer be
Nematode sick soil, the sick soil dosage compared to the single sick soil inoculation technique of existing root-knot nematode reduce 1/2-2/3, greatly reduce sick soil
Acquire, transport, expand it is numerous culture etc. workload and cost.
4, the present invention is placed on middle level per potting nematode sick soil, and levels are no nematode soil, single compared to existing root-knot nematode
One sick soil inoculation technique, the present invention can effective protection inoculation worm sources be prevented from losing, not by dry tack free and it is sunlit influence,
Reduce the loss and death of inoculation worm sources.
5, upper layer of the present invention is no nematode soil, and in transplanting, seedling kind is on upper layer without in sick soil.Compared to existing root knot line
The single sick soil inoculation technique of worm, the seedling that present invention energy effective protection has just been transplanted is not by nematode infection, after Seeding planting survives,
Root is just grown into the nematode soil of middle level, not only can guarantee be inoculated with successfully in this way, but also is avoided that seedling dies of illness too early.
6, the present invention adds a certain proportion of decomposed manure as base manure, is planted for potting in no root-knot nematode soil
The growth of strain provides nutrition and ensures.
7, expand numerous pretreated sick soil insect density to increase, success ratio of inoculation also increases, and it is pre- through mixing to expand numerous sick soil
It is inoculated with by same amount after processing, keeps inoculation condition consistent, it is ensured that the accuracy and comparativity of experiment.
8, inoculation flowerpot inner wall is divided into 3 layers of upper, middle and lower, convenient layering dress in proportion with graduation mark by 3 layers of native usage ratio
Soil, making dress soil, operation is simple.
Description of the drawings
Fig. 1 is the structural schematic diagram for being inoculated with flowerpot.Fig. 2 is first embodiment tomato root-knot eelworm effect of inoculation figure.
Fig. 3 is that first embodiment tomato root-knot eelworm is inoculated with pot experiment figure.
Fig. 4 is second embodiment muskmelon root-knot nematode effect of inoculation figure.
Fig. 5 is that second embodiment muskmelon root-knot nematode is inoculated with pot experiment figure.
Drawing reference numeral in figure is：1- pot bodies, 2- graduation marks.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.
Embodiment 1
3 layers of sick soil inoculation test of tomato root-knot eelworm potting, include the following steps：
（1）No sick soil acquisition and processing
Decomposed Swine manure is added without root-knot nematode pool mud soil in acquisition after crushed, and it is spare to obtain no sick soil after mixing, rotten
The volumetric usage ratio of ripe Swine manure and no root-knot nematode pool mud soil is 1：10..
（2）Sick soil acquires and processing
There are the old complaint and rhizosphere sick soil of root knot symptom in acquisition tomato root, and old complaint is cut to be broken into about 1cm broken section, by broken section of old complaint
It is spare it to be uniformly mixed so as to obtain sick soil in addition rhizosphere sick soil.
（3）Sick soil expands numerous culture
A, nursery：Commercially available special seedling medium is fitted into 50 hole seedling-growing containers, commercially available ruby tomato species are sown into seedling medium
In, per 1, nursery hole seed, transplanted when tomato seedling grows to 4-5 true leaves.
B, expand numerous culture：Step（2）The spare sick soil of acquisition mixing is dispensed into flowerpot, the tomato that step A is cultivated
Transplantation of seedlings is on sick soil, and per 3 plants of basin, after seedling grows to 35 days, nematode completes the breeding of 1 generation, the root-knot nematode in soil obtain expanding it is numerous,
Insect density increases.
（4）Expand numerous sick soil pretreatment
Before expanding numerous sick soil pretreatment, the potting for expanding numerous culture shifts to an earlier date stopping water drenching in 7 days.Step B is expanded to the potting kind of numerous culture
Eggplant cauline leaf is cut, and old complaint and sick soil are poured out, and will be had the old complaint of root knot to cut and is broken into broken section of about 1cm, and mixing in sick soil is added and obtains
It is spare to pretreatment sick soil.
（5）3 layers of sick soil inoculation
The plastic flowerpot of diameter 30cm high 25cm is selected, inoculation flowerpot structure is as shown in Figure 1,1 inner wall of pot body is equipped with scale
Flowerpot graduation mark 2 is divided into 3 layers of the identical upper, middle and lower of volume by line 2 by 3 layers of native usage ratio.It is spent respectively in each inoculation
Basin bottom part first adds one layer of step（1）Processed no sick soil, the second layer add step（4）Processed pretreatment sick soil, topmost
Again plus one layer of step（1）Processed no sick soil, 3 layer soil bodies accumulate amount ratio：Without sick soil：Pre-process sick soil：No sick soil is 1：1：
1。
（6）Transplanting
Transplanting inoculation is carried out after installing basin, tomato 4-5 true leaf age seedling replantings on flowerpot upper layer without in sick soil, every 1 plant of basin is total
40 basins do not apply fertilizer after transplanting, do not apply nematode killing agent, only carry out conventional water drenching and overground part disease pest management.
（7）Control treatment
If the single sick soil inoculation of root-knot nematode is control, step（4）Processed pretreatment sick soil is directly loadable into same specification
Plastic flowerpot in, totally 40 basin, the transplanting of tomato seedling and the same step of management（6）.
（8）Test results and analysis
60 days investigation tomato plant death rates after transplanting, and each potted tomato root is acquired respectively, investigate root-knot nematode incidence
And root knot disease grade.1 test result of table shows the 40 basins experiment tomato of 3 layers of sick soil inoculation, and the dead strain number of tomato is 0, the death rate 0,
Root-knot nematode incidence is 100%, and sick grade of falling ill is mostly 3-5 grades, wherein at most with 4 grades.It is inoculated with work with through expanding numerous single sick soil
Control, the dead strain number of tomato are 6 plants, and the death rate 15.0%, root-knot nematode incidence is 100%, and root knot disease grade is 3-5 grades, wherein
At most with 5 grades.In contrast, when same with expanding numerous sick soil inoculation, single sick soil inoculation is dead compared with the tomato of 3 layers of sick soil inoculation
Rate is high, morbidity weight.（Test result refers to table 1, and effect of inoculation is shown in that Fig. 2, inoculation pot experiment are shown in Fig. 3）
Root knot grade scale：
0 grade：Root system is without root knot
1 grade：The 1% ~ 5% of root system has root knot
2 grades：The 6% ~ 20% of root system has root knot
3 grades：The 21% ~ 40% of root system has root knot
4 grades：The 41% ~ 70% of root system has root knot
5 grades：71% or more of root system has root knot.
Embodiment 2
3 layers of sick soil inoculation test of muskmelon root-knot nematode potting, include the following steps：
（1）No sick soil acquisition and processing
It acquires and Chicken dung organic fertilizer is added after the wasteland soil without root-knot nematode crushes, it is spare to obtain no sick soil after mixing, decomposed
The volumetric usage ratio of chicken manure organic fertilizer and no root-knot nematode wasteland soil is 1：10.
（2）Sick soil acquires and processing
There are the old complaint and rhizosphere sick soil of root knot symptom in acquisition muskmelon root, and old complaint is cut and is broken into broken section of 1-2cm, and old complaint is added for broken section
Enter to be uniformly mixed so as to obtain root-knot nematode sick soil in rhizosphere sick soil spare.
（3）Sick soil expands numerous culture
A, nursery：Commercially available special seedling medium is fitted into 50 hole seedling-growing containers, commercially available northern No. 1 muskmelon kind of sweet tea is sown into seedling medium
In, per 1, nursery hole seed, transplanted when muskmelon seedling grows to 3-4 true leaves.
B, expand numerous culture：Step（2）The spare sick soil of acquisition mixing is dispensed into flowerpot, the muskmelon that step A is cultivated
Transplantation of seedlings is on sick soil, and per 3 plants of basin, after seedling grows to 30 days, nematode completes the breeding of 1 generation, the root-knot nematode in soil obtain expanding it is numerous,
Insect density increases.
（4）Expand numerous sick soil pretreatment
Before expanding numerous sick soil pretreatment, the potting for expanding numerous culture shifts to an earlier date stopping water drenching in 5 days.Step B is expanded to the potting sweet tea of numerous culture
Melon cauline leaf is cut, and old complaint and sick soil are poured out, and will be had the old complaint of root knot to cut and is broken into broken section of 1-2cm, and disease is added for broken section in old complaint
It is spare that pretreatment sick soil is uniformly mixed so as to obtain in soil.
（5）3 layers of sick soil inoculation
The plastic flowerpot of diameter 30cm high 25cm is selected, inoculation flowerpot structure is as shown in Figure 1,1 inner wall of pot body is equipped with scale
Flowerpot graduation mark 2 is divided into 3 layers of the identical upper, middle and lower of volume by line 2 by 3 layers of native usage ratio.It is spent respectively in each inoculation
Basin bottom part first adds one layer of step（1）Processed no sick soil, the second layer add step（4）Processed pretreatment sick soil, topmost
Again plus one layer of step（1）Processed no sick soil, 3 layer soil bodies accumulate amount ratio：Without sick soil：Pre-process sick soil：No sick soil is 1：1：
1。
（6）Transplanting
Transplanting inoculation is carried out after installing basin, muskmelon 3-4 true leaf age seedling replantings on flowerpot upper layer without in sick soil, every 2 plants of basin is total
40 basins do not apply fertilizer after transplanting, do not apply nematode killing agent, only carry out conventional water drenching and overground part disease pest management.
（7）Control treatment
If the single sick soil inoculation of root-knot nematode is control, step（2）Processed sick soil is directly loadable into diameter 30cm high 25cm
Plastic flowerpot in（Without expanding numerous processing）, totally 40 basin, the transplanting of muskmelon seedling and the same step of management（6）.
（8）Test results and analysis
55 days investigation Muskmelon Plants death rates after transplanting, and each potting muskmelon root is acquired respectively, investigate root-knot nematode incidence
And root knot disease grade.2 test result of table shows the 40 basins experiment muskmelon of 3 layers of sick soil inoculation, and the plant death rate is 0, root-knot nematode hair
Sick rate is 100%, and sick grade of falling ill is mostly 3-5 grades, wherein at most with 4 grades.It is compared with without expanding numerous single sick soil inoculation, sweet tea
The dead strain number of melon is 9 plants, the death rate 11.25%, and root has no that root knot symptom has 5 plants, and root-knot nematode incidence is 93.75%, root
The sick grade of knot is mostly 3-5 grades, wherein at most with 4 grades.In contrast, when compareing single sick soil inoculation with numerous sick soil inoculation is not expanded, hair
Sick rate is light compared with what 3 layers of sick soil were inoculated with, and also has and do not fall ill on a small quantity, may be relatively low related with the insect density that does not expand numerous sick soil.（Examination
It tests the results detailed in Table 2, effect of inoculation is shown in that Fig. 4, inoculation pot experiment are shown in Fig. 5, and root knot grade scale is the same as example 1）
It should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention.In addition, it should also be understood that, readding
After having read the content of the invention lectured, those skilled in the art can various modifications may be made or change to the present invention, these are of equal value
Form is also fallen within the scope of the appended claims of the present application.
Claims (6)
1. a kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting, which is characterized in that include the following steps：
（1）No sick soil acquisition and processing
Acquisition pool mud soil, wasteland soil or plant root have no root knot symptom without root-knot nematode soil, being added after crushing decomposed has
No sick soil is spare as being obtained after base manure, with no root-knot nematode soil mixing for machine fertilizer, the volume ratio of organic fertilizer and no root-knot nematode soil
It is 1：10；
（2）Sick soil acquires and processing
There are the old complaint and rhizosphere sick soil of root knot symptom in herborization root, and old complaint is cut in broken addition rhizosphere sick soil and is uniformly mixed so as to obtain disease
Soil is spare；If plant root has the old complaint of root knot symptom to rot, directly it is spare to be uniformly mixed so as to obtain sick soil for acquisition rhizosphere sick soil；
（3）Sick soil expands numerous culture
A, nursery：Commercially available special seedling medium is fitted into 50 hole seedling-growing containers, susceptible host's seed of root-knot nematode is sown into and is educated
In seedling matrix, per 1, nursery hole seed, transplanted when seedling grows to 3-5 true leaves；
B, expand numerous culture：Step（2）The spare sick soil of acquisition mixing is divided in flowerpot, and the step A transplantation of seedlings cultivated is existed
On sick soil, per 3 plants of basin 1-, after seedling grows to 30-60 days, nematode breeds 1-2 generations, and the root-knot nematode in soil obtains expanding numerous, insect population
Density increases；
（4）Expand numerous sick soil pretreatment
Potting host's overground part cauline leaf that step B is expanded to numerous culture is cut, and root and sick soil are poured out, and will have the old complaint of root knot to cut broken
It is spare that pretreatment sick soil is uniformly mixed so as to obtain in addition sick soil；
（5）3 layers of sick soil inoculation
First add one layer of step in inoculation flowerpot bottom（1）Processed no sick soil, the second layer add step（4）Processed pretreatment
Sick soil topmost adds one layer of step again（1）Processed no sick soil directly carries out transplanting inoculation after installing basin, and host 3-5 is true
Leaf age seedling replanting is on upper layer without in sick soil.
2. preparation method as described in claim 1, it is characterised in that：In step A, the susceptible host of root-knot nematode is kind
Eggplant, muskmelon or cucumber.
3. preparation method as described in claim 1, it is characterised in that：Step（4）In, before expanding numerous sick soil pretreatment, expand numerous
The potting of culture shifts to an earlier date 4-7 days and stops water drenching.
4. preparation method as described in claim 1, it is characterised in that：Step（5）In, 3 layer soil bodies accumulate amount ratio：Without sick soil：
Pre-process sick soil：No sick soil is 1：1：1.
5. preparation method as described in claim 1, it is characterised in that：Step（5）In, the inoculation flowerpot inner wall sets scale
Line.
6. preparation method as claimed in claim 5, it is characterised in that：The graduation mark by flowerpot be divided into volume it is identical on
In lower 3 layers.
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| CN110250113A (en) * | 2019-07-09 | 2019-09-20 | 云南农业大学 | A method of M hapla is cultivated using Eupatorium adenophorum |
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| CN110250113A (en) * | 2019-07-09 | 2019-09-20 | 云南农业大学 | A method of M hapla is cultivated using Eupatorium adenophorum |
| CN110250113B (en) * | 2019-07-09 | 2021-05-25 | 云南农业大学 | Method for culturing Meloidogyne incognita by using Eupatorium adenophorum |
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