CN101731103A - Artificial inoculation method of root-knot nematodes at upper part of tomato plant field - Google Patents
Artificial inoculation method of root-knot nematodes at upper part of tomato plant field Download PDFInfo
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Abstract
The invention discloses an artificial inoculation method of root-knot nematodes at the upper part of a tomato plant field, which comprises the steps of disinfecting the inoculation part at the upper part of the tomato field and inoculating suspension containing second instar larvae of the root-knot nematodes to the inoculation part, and the inoculation effect can be checked after 8 days at the earliest. The appropriate inoculation materials of the root-knot nematodes are selected for matching with the materials of different growth periods of leaves, buds and stems of tomato plants; and the invention further provides a preparation scheme of the suspension of the root-knot nematodes for inoculation and a culture scheme of inoculated tomatoes, determines surface disinfection, artificial inoculation and moisturizing technologies and establishes the root-knot nematode inoculation method at the leaves, the buds and the stems of the tomatoes, which has the advantages of easy operation, intuitive inoculation result, repeated inoculation and targeted quantitative inoculation, simpleness, easy operation, and easy popularization.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of tomato root-root-knot nematode and make system mutually, be specifically related to the method for tomato plant overground part artificial infection root-knot nematode.
Background technology
Root-knot nematode (Meloidogyne spp) can parasitic 2000 various plants, produce to crops and bring heavy losses.Breeding for disease resistance is the essential measure of this disease of control.The classical symptom of plant root-knot nematode disease is, in the root formation root knot of host plant.Histopathological study shows: root knot inside also has the megabacterium that is developed into by host cell except that the root-knot nematode that arranged the different length of times, the root-knot nematode set colonizes in the plant corpus, absorbs nutrient component in megabacterium, up to finishing the history of life.The volume of the interior megabacterium of root knot is about 50～400 times of normal root cell size, and its plant nucleic acid that contains, protein component are multiplied.
In recent years, it is the focus of research both at home and abroad that root-knot nematode and host plant interact, both at home and abroad to the formation of root knot with keep closely-related gene and carried out comparatively deep research.The reticent effect of portion gene is shown that behind the gene silencing, can reduce the root knot percentage of host plant, this is that the plant variety of utilizing transgenic technology to cultivate anti-root-knot nematode has been opened up new approach.
Tomato is global important vegetables, and the tomato root-knot eelworm disease generally takes place, and after first plant resistive connection nematode Mi gene was found in the wild-type tomato plant, each state was all carrying out the anti-root-knot nematode research of tomato.Be the novel nematode killing agent evaluating drug effect that object is carried out with the tomato root-knot eelworm disease, also underway always.
It is the important object that people carry out above research that tomato root-root-knot nematode makes system mutually always.For the ease of observing the inoculation result, set up the tomato root-root-knot nematode inoculation technique of sand culture, water planting, tissue culture in succession.These inoculation systems have his own strong points, but have following defective respectively:
1, tomato root-root-knot nematode sand culture technology
Tomato root-root-knot nematode sand culture technology is widely used technology, the major technique link be with tomato species in sand, root-knot nematode is seeded in the tomato root.The defective of this technology is the uncertainty of inoculation position, is filled in the plant root and inoculates owing to adopt root-knot nematode suspension watered, so that tip of a root can form root knot on earth, is difficult to expect.When checking root knot, root must be taken out from soil, behind the wash clean, could observe, tend to damage the root of plant like this, cause the root can not regrowth.In addition, in inoculation, because the root growth process is not directly perceived, people often can only inoculate 1 time, and repeated inoculation and fixed point inoculation almost is impossible thing.Because the uncertainty of root-knot nematode time of infection in the inoculation system, often can only 30 days after, could check and inoculate the result.
2, tomato root-root-knot nematode water culture technology
In order to observe the evolution of root knot, people utilize water culture technology to cultivate tomato root-root-knot nematode system.The key link of this technology is that postvaccinal tomato plant is cultivated in nutrient solution, this technology has and is easy to observe the root knot evolution, but exist the defective that is difficult to repeated inoculation and fixed point inoculation equally with the sand culture technology, water culture technology is also than sand culture technical sophistication simultaneously.
In sum, prior art all be with 2 age root-knot nematode be inoculated into the tomato root, induce the tomato root to form root knot.Because the various matrix of growth needs of root, in a single day root enters matrix, and the root knot of formation is difficult for repeated observation, carry out repeatedly repeated inoculation and the site-directed quantitative inoculation of tomato root root-knot nematode, almost is impossible thing.In view of tomato plant can be grown at the position on the ground continuously, therefore be badly in need of to develop the ripe tomato plant position artificial infection on the ground root-knot nematode technology of a cover, satisfy the needs of research and production department, for set up fast, the root-knot nematode prophylactico-therapeutic measures of environmental protection contributes.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, especially at the evaluation of resistance of tomato to root-knot nematode, control root-knot nematode drug effect detects, the inoculation result that tomato root-root-knot nematode that tomato and root-knot nematode make signal detection mutually does in the system mutually and run into is not directly perceived, complex operation, cultivating system pollutes easily, be difficult to fixed point, technical problems such as quantitative inoculation, the method of a kind of tomato plant overground part artificial infection root-knot nematode is provided, sets up operation easily, the inoculation visual result, can repeated inoculation and the tomato leaf of site-directed quantitative inoculation, bud, stem's root-knot nematode inoculation method.
Purpose of the present invention is achieved by following technical proposals:
The method of a kind of tomato plant overground part artificial infection root-knot nematode is provided, after the surface sterilization of tomato overground part inoculation position, root-knot nematode 2 instar larvae suspension is inoculated into inoculation position, can check effect of inoculation behind the certain hour of preserving moisture.
Preferably, tomato overground part inoculation position can select to transplant the 3rd tomato leaf under the terminal bud of the tomato plant after 2 weeks, the terminal bud of transplanting the tomato plant after 2 weeks down the 1st tomato leaflet tablet petiole and stem intersection or transplanted for 2 weeks after tomato plant ground position 2cm stem highly.
The inventive method specifically may further comprise the steps:
(1) cultivates the inoculation tomato seedling;
Potassium permanganate with 0.5% soaks seedling-cultivating tray 24h, carries out surface sterilization.Susceptible tomato seeds with 6% clorox surface sterilization 10min after, alcohol surface sterilization 2min with 75%, with sterilization washing 5 times, sowing is in the seedling-cultivating tray that the mixed-matrix of sterilizing is housed, and seed sprouting was transplanted to tomato seedling in the plastic basin that the mixed-matrix of sterilizing is housed after 2 weeks, the plastic basin diameter is preferably 20cm, 1 of every pot transplanting, water every day, executes weekly and water nitrogen (N): phosphorus (P): potassium (K) is 20: 20: 20 nutrient solution (NPK) 1 time.Tomato seedling after the transplanting is placed on (25 ℃～31 ℃, relative moisture 65%～95%) in the greenhouse.Described mixed-matrix is the mixture that mixes at 4: 1 according to volume ratio for husky and soil.
(2) preparation inoculation root-knot nematode 2 instar larvae suspension;
With the egg capsule of root-knot nematode with 0.5% hypochlorous acid receive carry out surface sterilization 5min after, add the aqua sterilisa dilution of 5 times of volumes, with 8000 rev/mins of centrifugal 5min of kind speed, sterilization washing 4 times, be placed under the room temperature and hatch 48h,, collect 2 instar larvaes with the centrifugal 4min of 3000 rev/mins speed, be mixed with the concentration of 2000/mL with aqua sterilisa, now with the current.
(3) inoculate root-knot nematode in the enterprising pedestrian worker of tomato leaf:
The 3rd tomato leaf under the terminal bud of the tomato plant after preferred 2 weeks of transplanting, behind 70% alcohol surface sterilization 30s, with sterilization washing 4 times, under the room temperature behind the natural air drying, puncture epidermis 5～6 places of leaf along vein with the fine needle of sterilization, do not pierce through blade, the root-knot nematode 2 instar larvae suspension of every leaf Dropwise 50 μ L step (2) preparation wherein contain 100 root-knot nematode 2 instar larvaes alive.Preserve moisture with preservative film parcel whole blade.Preferably, place the cotton balls that is added with aqua sterilisa at the petiole position and strengthen preserving moisture, every 4h adds water once on cotton balls, preserved moisture 4 days.
Perhaps carry out the root-knot nematode artificial infection in tomato bud root:
The 1st tomato leaflet tablet petiole under the preferred terminal bud of transplanting the tomato plant after 2 weeks and stem intersection, behind 70% alcohol surface sterilization 1min, with sterilization washing 4 times, under the room temperature behind the natural air drying, with the micro syringe after the sterilization, inject the root-knot nematode 2 instar larvae suspension of 50 μ L steps (2) preparation, wherein contain 100 root-knot nematode 2 instar larvaes alive, injection depth be no more than institute's injection site terminal bud diameter half, wrap up whole bud with preservative film and preserve moisture.Preferably, place the cotton balls that is added with aqua sterilisa in the bottom of injection orifice and preserve moisture, every 4h adds water once on cotton balls, preserved moisture 4 days.
Perhaps carry out the root-knot nematode artificial infection in tomato stem position:
The stem of the tomato plant ground position 2cm height after 2 weeks is transplanted in selection, behind 70% alcohol surface sterilization 2min, with sterilization washing 4 times, under the room temperature behind the natural air drying, fine needle with sterilization stings 3 apertures in the same direction of stem, every hole depth be no more than the position diameter stem that stings half, distance between per two holes is preferably 3.0mm, with the micro syringe after the sterilization, give and go up the root-knot nematode 2 instar larvae suspension that the preparation of 50 μ L steps (2) is injected in the hole most, wherein contain 100 root-knot nematode 2 instar larvaes alive, wrap up inoculation position with preservative film, three holes are all wrapped up and being preserved moisture.Preferably, place the cotton balls that is added with aqua sterilisa in the bottom of bottom injection orifice and preserve moisture, every 4h adds water once on cotton balls, preserved moisture preservative film wrapping portion cotton balls 4 days.
Postvaccinal tomato seedling is placed in the greenhouse, and in the greenhouse 25 ℃～31 ℃, relative moisture 65%～95%.
Inoculated result's inspection after preserving moisture several days.Specifically be the small root-knot nematode insect gall that just can see formation at the artificial infection tomato leaf after 8 days on the blade face, later insect gall increases gradually, after 27 days, can carry out complete observation.As seen there is typical grain of rice shape insect gall on the blade face along vein, under 40 times of anatomical lens, and strip off insect gall, female worm polypide of visible white root-knot nematode and yellow egg capsule.
Artificial infection tomato bud on the stem and petiole of visible inoculation place, formed the root-knot nematode insect gall after 27 days, under 40 times of anatomical lens, and strip off insect gall, female worm polypide of visible white root-knot nematode and yellow egg capsule.
Artificial infection tomato stem formed the root-knot nematode insect gall after 27 days on the stem of visible inoculation place.
The invention has the beneficial effects as follows:
The invention provides the method for a kind of brand-new tomato plant overground part artificial infection root-knot nematode, overcome the limitation of prior art, especially at the evaluation of resistance of tomato to root-knot nematode, control root-knot nematode drug effect detects, the inoculation result that tomato root-root-knot nematode that tomato and root-knot nematode make signal detection mutually does in the system mutually and run into is not directly perceived, complex operation, cultivating system pollutes easily, be difficult to fixed point, technical problems such as quantitative inoculation, the present invention selects suitable root-knot nematode inoculation material to cooperate the different leaf of tomato plant, bud, stem's material in vegetative period, the inoculation preparation of root-knot nematode suspension and the cultivation scheme of inoculating tomato are provided, determined surface sterilization, the artificial infection and the technology of preserving moisture, set up easy operation, get final product result directly perceived after inoculating 8 days the earliest, and can repeated inoculation and the tomato leaf of site-directed quantitative inoculation, bud, stem's root-knot nematode inoculation method, simple, be easy to promote.
Description of drawings
Fig. 1 tomato leaf-root-knot nematode inoculation result
Fig. 2 tomato bud-root-knot nematode inoculation result
Fig. 3 tomato stem-root-knot nematode inoculation result
Embodiment
Further describe the present invention below in conjunction with the drawings and specific embodiments.
Embodiment 1
(1) cultivates the inoculation tomato seedling;
Potassium permanganate with 0.5% soaks seedling-cultivating tray 24h, carries out surface sterilization.(buy from seeds company, 95.0% above tomato variety is all susceptible in the market, can select native breed for susceptible tomato seeds.What present embodiment used is " No. 1, jewel " tomato that Agricultural University Of South China produces) with behind 6% the clorox surface sterilization 10min, alcohol surface sterilization 2min with 75%, with sterilization washing 5 times, sowing is in the seedling-cultivating tray that the mixed-matrix of sterilizing is housed, seed sprouting is after 2 weeks, tomato seedling is transplanted in the plastic basin that the mixed-matrix of sterilizing is housed, the plastic basin diameter is preferably 20cm, 1 of every pot transplanting, water every day, executes weekly and water nitrogen (N): phosphorus (P): potassium (K) is 20: 20: 20 nutrient solution 1 time.Tomato seedling after the transplanting is placed on (25 ℃～31 ℃, relative moisture 65%～95%) in the greenhouse.Described mixed-matrix is the mixture that mixes at 4: 1 according to volume ratio for husky and soil.
(2) preparation inoculation root-knot nematode 2 instar larvae suspension;
With the egg capsule of root-knot nematode with 0.5% hypochlorous acid receive carry out surface sterilization 5min after, add the aqua sterilisa dilution of 5 times of volumes, with 8000 rev/mins of centrifugal 5min of kind speed, sterilization washing 4 times, be placed under the room temperature and hatch 48h,, collect 2 instar larvaes with the centrifugal 4min of 3000 rev/mins speed, be mixed with the concentration of 2000/mL with aqua sterilisa, now with the current.
The egg capsule propagation method of described root-knot nematode can be with reference to existing routine techniques.The method that provides in for example Wang Xin honor " risk analysis of Chinese rose nematode and the research of anti-root-knot nematode genetic mechanism " (Guangzhou: Agricultural University Of South China, 2000, doctorate paper).Tomato seeds with 6% clorox surface sterilization 10min after, the alcohol surface sterilization 2min with 75%, with sterilization washing 5 times, sowing is in the seedling-cultivating tray that the mixed-matrix of sterilizing is housed, seedling-growing container is sterilized with 0.5% potassium permanganate.When treating 3 leaves of tomato seedling length to the, being transplanted to volume is that the single egg capsule of picking Meloidogyne incognita is inoculated into root then in the small flower that sterilization soil is housed of 0.25L.Enlarged culture was 1 time in per 2 months.During enlarged culture, the tomato root of severe infections root-knot nematode is equally divided into 10 parts, is inoculated into respectively in 10 small flowers of planting tomato, allow it produce enough egg capsules.
(3) inoculate root-knot nematode in the enterprising pedestrian worker of tomato leaf:
The 3rd tomato leaf under the terminal bud of the tomato plant after preferred 2 weeks of transplanting, behind 70% alcohol surface sterilization 30s, with sterilization washing 4 times, under the room temperature behind the natural air drying, puncture epidermis 5～6 places of leaf along vein with the fine needle of sterilization, do not pierce through blade, the root-knot nematode 2 instar larvae suspension of every leaf Dropwise 50 μ L step (2) preparation wherein contain 100 root-knot nematode 2 instar larvaes alive.Preserve moisture with preservative film parcel whole blade.Inoculation contrast blade is established in every processing of while, Dropwise 50 μ L sterilization deionized water on the contrast blade, and all the other are identical with the processing blade.Preferably, place the cotton balls that is added with aqua sterilisa at the petiole position and strengthen preserving moisture, every 4h adds water once on cotton balls, preserved moisture 4 days.
Behind the artificial infection tomato leaf 8 days, form small root-knot nematode insect gall on the visible blade face, later insect gall increases gradually, after 27 days, there is typical grain of rice shape insect gall on visible blade face along vein, under 40 times of anatomical lens, the strip off insect gall, female worm polypide of visible white root-knot nematode and yellow egg capsule.See shown in the accompanying drawing 1.In the accompanying drawing 1, A is contrast, and s is an inoculation position; B is tomato leaf-root-knot nematode inoculation result, and g is an insect gall; C is a root-knot nematode insect gall anatomical results on the tomato leaf, and f is the female worm of root-knot nematode (50 times), and e is root-knot nematode egg capsule (50 times).
Step (1) and step (2) are with embodiment 1.
Step (3) is carried out the root-knot nematode artificial infection in tomato bud root:
The 1st tomato leaflet tablet petiole under the terminal bud of the tomato plant after transplanting for 2 weeks and stem intersection, behind 70% alcohol surface sterilization 1min, with sterilization washing 4 times, under the room temperature behind the natural air drying, with the micro syringe after the sterilization, inject the root-knot nematode 2 instar larvae suspension of 50 μ L steps (2) preparation, wherein contain 100 root-knot nematode 2 instar larvaes alive, injection depth be no more than institute's injection site terminal bud diameter half, wrap up whole bud with preservative film and preserve moisture.Preferably, place the cotton balls that is added with aqua sterilisa in the bottom of injection orifice and preserve moisture, every 4h adds water once on cotton balls, preserved moisture 4 days.
Artificial infection tomato bud on the stem and petiole of visible inoculation place, formed the root-knot nematode insect gall after 27 days, under 40 times of anatomical lens, and the strip off insect gall, female worm polypide of visible white root-knot nematode and yellow egg capsule are seen shown in the accompanying drawing 2.Dropwise 50 μ L sterilization deionized water on the contrast bud, all the other are identical with the processing bud.In the accompanying drawing 2, A is contrast, and s is an inoculation position; B is tomato bud-root-knot nematode inoculation result, and g is an insect gall; C goes up root-knot nematode insect gall anatomical results in the tomato bud; F is the female worm of root-knot nematode (40 times), and e is root-knot nematode egg capsule (40 times).
Embodiment 3
Step (1) and step (2) are with embodiment 1.
Step (3) is carried out the root-knot nematode artificial infection in tomato stem root position:
The stem of the tomato plant ground position 2cm height after 2 weeks is transplanted in selection, behind 70% alcohol surface sterilization 2min, with sterilization washing 4 times, under the room temperature behind the natural air drying, fine needle with sterilization stings 3 apertures in the same direction of stem, every hole depth be no more than the position diameter stem that stings half, distance between per two holes is preferably 3.0mm, with the micro syringe after the sterilization, give and go up the root-knot nematode 2 instar larvae suspension that the preparation of 50 μ L steps (2) is injected in the hole most, wherein contain 100 root-knot nematode 2 instar larvaes alive, preserve moisture with preservative film parcel injection site.Preferably, place the cotton balls that is added with aqua sterilisa in the bottom of bottom injection orifice and preserve moisture, every 4h adds water once on cotton balls, preserved moisture 4 days.Dropwise 50 μ L sterilization deionized water on the contrast stem, all the other are identical with the processing stem.
Artificial infection tomato stem can macroscopic examination inoculation result after 8 days.Forming the root-knot nematode insect gall after 27 days on the stem of inoculation place sees shown in the accompanying drawing 3.In the accompanying drawing 3, CK is contrast, and s is an inoculation position, and g is an insect gall.
According to the embodiment check result, can determine that the present invention has successfully set up the method for tomato plant overground part artificial infection root-knot nematodes such as artificial infection tomato leaf, bud, stem.Can create and be suitable for tomato tomato-root-knot nematode that the evaluation of resistance of root-knot nematode, the detection of control root-knot nematode drug effect, tomato and root-knot nematode make signal detection is mutually made system mutually, have easy operation, visual result, can repeated inoculation and site-directed quantitative inoculation and the remarkable advantage that shortens the observed result required time, effectively advance set up fast, the process of the root-knot nematode prophylactico-therapeutic measures of environmental protection.
Claims (10)
1. the method for a tomato plant overground part artificial infection root-knot nematode is characterized in that the suspension that will contain root-knot nematode 2 instar larvaes is inoculated into inoculation position, the processing of preserving moisture with after the surface sterilization of tomato overground part inoculation position.
2. according to the method for the described tomato plant overground part of claim 1 artificial infection root-knot nematode, it is characterized in that tomato overground part inoculation position for the terminal bud of transplanting following the 3rd tomato leaf of tomato plant terminal bud after 2 weeks, transplanting the tomato plant after 2 weeks down the 1st tomato leaflet tablet petiole and stem intersection or transplanted for 2 weeks after tomato plant ground position 2cm stem highly.
3. according to the method for the described tomato plant overground part of claim 1 artificial infection root-knot nematode, it is characterized in that may further comprise the steps:
(1) germinates being seeded in the mixed-matrix after the susceptible tomato seeds sterilization; After the germination tomato seedling is transplanted, cultivate the inoculation tomato seedling;
(2) with after the egg capsule surface sterilization of the root-knot nematode dilution centrifugal treating, under room temperature, hatch 48h, collect 2 instar larvaes after the centrifugal treating, be mixed with the suspension of 2000/mL concentration, promptly get and inoculate with root-knot nematode 2 instar larvae suspension with aqua sterilisa;
(3) the root-knot nematode 2 instar larvae suspension with step (2) preparation are inoculated in 2 week of transplanting back tomato overground part inoculation positions; The inoculation position processing of preserving moisture.
4. according to the method for the described tomato plant overground part of claim 3 artificial infection root-knot nematode, it is characterized in that the described susceptible tomato seeds sterilization of step (1) is with behind 6% the clorox surface sterilization 10min, alcohol surface sterilization 2min with 75% is with sterilization washing 5 times.
5. according to the method for the described tomato plant overground part of claim 3 artificial infection root-knot nematode, it is characterized in that the described mixed-matrix of step (1) is the mixture that mixes at 4: 1 according to volume ratio for husky and soil.
6. according to the method for the described tomato plant overground part of claim 3 artificial infection root-knot nematode, the egg capsule surface sterilization that it is characterized in that the described root-knot nematode of step (2) is to receive with 0.5% hypochlorous acid to carry out surface sterilization 5min.
7. according to the method for the described tomato plant overground part of claim 3 artificial infection root-knot nematode, it is characterized in that the described inoculation position of step (3) is the 3rd tomato leaf under the terminal bud of transplanting the tomato plant after 2 weeks; Inoculation method is the alcohol surface sterilization blade with 70%, and natural air drying under the room temperature behind the aqua sterilisa washing blade punctures the epidermis of leaf, every leaf Dropwise 50 μ L root-knot nematode 2 instar larvae suspension with the fine needle of sterilization along vein; Described preserving moisture is to wrap up whole blade with preservative film.
8. according to the method for the described tomato plant overground part of claim 3 artificial infection root-knot nematode, it is characterized in that the described inoculation position of step (3) is the 1st tomato leaflet tablet petiole under the terminal bud of transplanting the tomato plant after 2 weeks and stem intersection; Inoculation method is the alcohol surface sterilization inoculation position with 70%, and natural air drying under the room temperature of aqua sterilisa washing back adopts the sterilization micro syringe to inject 50 μ L root-knot nematodes, 2 instar larvae suspension; Described preserving moisture is to wrap up whole bud with preservative film.
9. according to the method for the described tomato plant overground part of claim 3 artificial infection root-knot nematode, it is characterized in that the stem of the tomato plant ground position 2cm height after the described inoculation position of step (3) is for 2 weeks of transplanting; Inoculation method is the alcohol surface sterilization stem with 70%, at 3 apertures of same direction thorn of stem, injects 50 μ L root-knot nematodes, 2 instar larvae suspension in going up most the hole; Described preserving moisture is to preserve moisture with preservative film parcel injection site.
10. according to the method for claim 7,8 or 9 described tomato plant overground part artificial infection root-knot nematodes, it is characterized in that placing the cotton balls that is added with aqua sterilisa near the described employing inoculation position of preserving moisture, every 4h adds water once on cotton balls, preserved moisture 4 days.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104623707A (en) * | 2015-01-14 | 2015-05-20 | 广西大学 | Surface sterilization method for maintaining hatching activity of root-knot nematode eggs |
| CN105379538A (en) * | 2015-11-20 | 2016-03-09 | 四川农业大学 | Method for restraining growth of tobacco axillary buds by injecting vegetable oil to degreasing cotton through syringe |
| CN106305103A (en) * | 2016-08-24 | 2017-01-11 | 梁文涛 | Cultivation method for reducing root-knot nematodes of tomatoes |
| CN108668831A (en) * | 2018-05-18 | 2018-10-19 | 广西壮族自治区农业科学院植物保护研究所 | A kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting |
-
2009
- 2009-11-27 CN CN2009101942150A patent/CN101731103B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104623707A (en) * | 2015-01-14 | 2015-05-20 | 广西大学 | Surface sterilization method for maintaining hatching activity of root-knot nematode eggs |
| CN104623707B (en) * | 2015-01-14 | 2017-11-28 | 广西大学 | A kind of processes for disinfecting surfaces for keeping root-knot nematode egg hatching activity |
| CN105379538A (en) * | 2015-11-20 | 2016-03-09 | 四川农业大学 | Method for restraining growth of tobacco axillary buds by injecting vegetable oil to degreasing cotton through syringe |
| CN106305103A (en) * | 2016-08-24 | 2017-01-11 | 梁文涛 | Cultivation method for reducing root-knot nematodes of tomatoes |
| CN108668831A (en) * | 2018-05-18 | 2018-10-19 | 广西壮族自治区农业科学院植物保护研究所 | A kind of 3 layers of sick soil inocalation method of plant root-knot nematode potting |
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| CN101731103B (en) | 2012-05-30 |
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