CN108658668A - A kind of preparation method and medicine fertilizer of liquid medicine fertilizer for hydroponic flowers - Google Patents
A kind of preparation method and medicine fertilizer of liquid medicine fertilizer for hydroponic flowers Download PDFInfo
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- CN108658668A CN108658668A CN201810674761.3A CN201810674761A CN108658668A CN 108658668 A CN108658668 A CN 108658668A CN 201810674761 A CN201810674761 A CN 201810674761A CN 108658668 A CN108658668 A CN 108658668A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a kind of preparation method of liquid medicine fertilizer for hydroponic flowers and medicine fertilizer, include the following steps：(1) by human or animal's excrement anaerobic fermentation, zymotic fluid, suction filtration is taken to obtain filtrate, carrying out reverse osmosis membrane to filtrate concentrates 7 10 times, obtains concentrate；(2) a certain amount of concentrate is taken, N fertilizer is added thereto and phosphate fertilizer, stirring and dissolving obtain solution A；(3) a certain amount of concentrate is taken, metallic element fertilizer and HEEDTA are added thereto, is stood after stirring and dissolving, obtains solution B；(4) solution A and solution B are mixed, adds biochemical fulvic acid, stirs evenly, be eventually adding ascorbic acid and adjust PH to 6 6.8, obtain finished medicines fertilizer；Wherein, often configuration 1L liquid medicine is fertile, and the addition of the N element is 90 110g, the addition of the P elements is 50 55g.It is an advantage of the current invention that improving the concentration of nutrient contained by biogas slurry, and biochemical fulvic acid and inorganic microelement are added thereto, so that it is preferably absorbed by plants, enhance the disease resistance of flowers.
Description
Technical field
The invention belongs to technical field of agricultural fertilizers, and in particular to a kind of preparation side of liquid medicine fertilizer for hydroponic flowers
Method and medicine fertilizer.
Background technology
In recent years, as economic society continues to develop, also day is increasingly for energy shortage that China faces and problem of environmental pollution
It is acute.Anaerobic fermentation technology can be used for solving energy and environmental problem, is widely used in China, especially rural area.
With the increase of anaerobic fermentation pond quantity and scale, the processing of residue biogas slurry and biogas residue is also more taken seriously.
Water culture technique is to use modern biotechnology, with physics, chemistry, biological engineering means, to common plant
It is tamed, it can long term growth in water.Compared with traditional earth culture flowers, hydroponic flowers sanitation and hygiene, maintenance side
Just, beautiful elegant, it is the first choice of household beautification, greening, it is deep to be liked by consumers in general.Contain in biogas slurry there are many nutriment,
It is contemplated that as the nutrient solution needed for hydroponic flowers growth, but the content of various nutrients in biogas slurry stoste, especially N, P
It is all relatively low, enough nutrients directly can not be provided for hydroponic flowers as nutrient solution.
Invention content
Technical problem to be solved by the present invention lies in：N, P content are relatively low in biogas slurry stoste, cannot be directly used as water planting flower
The nutrient solution of grass.
The present invention solves above-mentioned technical problem using following technical scheme：
A kind of preparation method of liquid medicine fertilizer for hydroponic flowers, includes the following steps：
(1) it takes zymotic fluid, vacuum filtration to obtain filtrate through anaerobic fermentation in human or animal's excrement, reverse osmosis is carried out to filtrate
Permeable membrane concentrates 7-10 times, obtains concentrate；
(2) a certain amount of concentrate is taken, nitrogenous fertilizer is added thereto and phosphate fertilizer, stirring and dissolving obtain solution A；
(3) a certain amount of concentrate is taken, metallic element fertilizer and HEEDTA are added thereto, stands, obtains after stirring and dissolving
Solution B；
(4) solution A and solution B are mixed, adds biochemical fulvic acid, stirs evenly, be eventually adding ascorbic acid adjusting
PH to 6-6.8 obtains finished medicines fertilizer；
Wherein, often configuration 1L liquid medicine is fertile, and the addition of the N element is 90-110g, the addition of the P elements is
50-55g。
Preferably, the preparation method of a kind of liquid medicine fertilizer for hydroponic flowers of the present invention, the metallic element
Fertilizer includes Fe elements, Zn elements, Cu elements, Mn elements.
Preferably, the preparation method of a kind of liquid medicine fertilizer for hydroponic flowers of the present invention often configures 1L liquid
Medicine fertilizer, it is the additive amount of 0.14-0.20g, Cu element that the additive amount of the Fe elements, which is the additive amount of 1.30-1.48g, Zn element,
For 0.01-0.02g, Mn element additive amount be 0.03-0.07g, the additive amount of the HEEDTA is 18-21g, described biochemical yellow rotten
The additive amount of acid is 30-35g.
Preferably, the preparation method of a kind of liquid medicine fertilizer for hydroponic flowers of the present invention, the nitrogenous fertilizer are urine
Plain, the described phosphate fertilizer is dipotassium hydrogen phosphate.
Preferably, the preparation method of a kind of liquid medicine fertilizer for hydroponic flowers of the present invention, it is described biochemical yellow rotten
The preparation method of acid is as follows：
A, aspergillus niger is activated on slant medium PDA, then shaking flask culture 2-3 days at 20-25 DEG C, mycelia is uniform
It is spare to obtain shake-flask seed liquid C for balling-up；Wherein, Shake flask medium is glucose 8-10g/L, wheat bran leaching liquor 25-27g/L；
B, geotrichum candidum is activated on slant medium PDA, then shaking flask culture 2-3 days at 25-27 DEG C, obtain shaking flask
Seed liquor D；Wherein, Shake flask medium is glucose 8-10g/L, wheat bran leaching liquor 25-27g/L；
C, after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, in 32-
Then shaking flask culture 15-20h at 35 DEG C presses 3:1:2 are mixed to get shake-flask seed liquid E；Wherein Shake flask medium is glucose sugar 32-
35g/L, beef peptone 13-15g/L, yeast extract 1.0-1.5g/L, sodium chloride 5-6g/L, potassium dihydrogen phosphate 0.5-0.8g/L,
PH7.0；
D, basal medium is configured, wherein molasses fermented alcohol effluent 700-750g/L, urea 5.0-6.0g/L, glucose are sugared
13-15g/L, surplus are water, and PH to 7 is adjusted with lye；
E, shake-flask seed liquid C, D, E are pressed 1:1:2 mixing are given birth to by inoculum concentration 5-7% access basic culture solution fermentations
The content for changing fulvic acid is the zymotic fluid of 25-28%.
Preferably, the preparation method of a kind of liquid medicine fertilizer for hydroponic flowers of the present invention, in the step d
The solid content of molasses fermented alcohol effluent is 45%-70%.
Preferably, the preparation method of a kind of liquid medicine fertilizer for hydroponic flowers of the present invention, it is described molasses fermented
The resting period of alcohol effluent is 3-5 months.
Preferably, the preparation method of a kind of liquid medicine fertilizer for hydroponic flowers of the present invention is sent out in the step e
Ferment condition is：Mixing speed 270-300r/min, 32-35 DEG C of fermentation temperature, fermentation period 55-65h.
The present invention also provides a kind of liquid medicine fertilizer for hydroponic flowers, use above-mentioned one kind for hydroponic flowers
The preparation method of liquid medicine fertilizer be prepared.
The technology of the present invention advantageous effect：
Technical solution of the present invention is concentrated biogas slurry by reverse osmosis membrane, improves the concentration of nutrient contained therein, and thereto
Biochemical fulvic acid and inorganic microelement is added, trace element is complexed using HEEDTA, so as to its more preferable ground cover plant
It absorbs；Biochemical fulvic acid not only has certain chelate ability, can also stablize the beneficial bacteria of water body digestive system, enhances
The disease resistance of flowers；
The biochemical fulvic acid that the present invention uses is that raw material progress microbial fermentation is made using blackstrap, after progress
Reason can be directly used for the configuration of medicine fertilizer, simple and convenient, saving of purchase cost, while also help environmental protection.
Specific implementation mode
For ease of those skilled in the art understand that technical solution of the present invention, in conjunction with specific embodiment to the technology of the present invention side
Case is described further.
The invention discloses a kind of amino acid pattern flower planting liquid medicine fertilizer, are prepared by following methods：
(1) human or animal's excrement takes zymotic fluid, is filtered by vacuum to obtain after precipitation through anaerobic fermentation at 20-40 DEG C
Filtrate is concentrated filtrate using reverse osmosis membrane, obtains concentrate.
For the suitable cycles of concentration of determination, the present invention first with reverse osmosis membrane by obtaining 2-10 times of concentrate, so
Nutrient in the concentrate of different multiples is measured respectively afterwards.Disappeared using the concentrated sulfuric acid-water to sample after boiling, it is triumphant using semimicro
The formula way of distillation measures full nitrogen, and molybdenum blue colorimetric method measures full phosphorus, and flare photometer measures full potassium.Concrete operation method is existing
Technology can refer to water and effluent monitoring analysis method.7-10 times is concentrated into biogas slurry stoste by analysis of experimental results determination.
(2) a certain amount of concentrate is taken, nitrogenous fertilizer is added thereto and phosphate fertilizer, stirring and dissolving obtain solution A；
(3) a certain amount of concentrate is taken, metallic element fertilizer and HEEDTA are added thereto, being stood after stirring and dissolving makes
HEEDTA is fully chelated with metallic element, obtains solution B；The metallic element fertilizer include Fe elements, Zn elements, Cu elements,
Mn elements；
(4) solution A and solution B are mixed, adds biochemical fulvic acid, stirs evenly, be eventually adding ascorbic acid adjusting
PH to 6-6.8 obtains finished medicines fertilizer；
Wherein, often configuration 1L liquid medicine is fertile, and the addition of the N element is 90-110g, the addition of the P elements is
50-55g, and nitrogenous fertilizer is urea, phosphate fertilizer is dipotassium hydrogen phosphate；The additive amount of the Fe elements is 1.30-1.48g, Zn element
Additive amount be 0.14-0.20g, Cu element additive amount be 0.01-0.02g, Mn element additive amount be 0.03-0.07g, it is described
The additive amount of HEEDTA is 18-21g, and the additive amount of the biochemical fulvic acid is 30-35g.
The preparation method of the biochemical fulvic acid is as follows：
A, aspergillus niger is activated on slant medium PDA, then shaking flask culture 2-3 days at 20-25 DEG C, mycelia is uniform
It is spare to obtain shake-flask seed liquid C for balling-up；Wherein, Shake flask medium is glucose 8-10g/L, wheat bran leaching liquor 25-27g/L；
B, geotrichum candidum is activated on slant medium PDA, then shaking flask culture 2-3 days at 25-27 DEG C, obtain shaking flask
Seed liquor D；Wherein, Shake flask medium is glucose 8-10g/L, wheat bran leaching liquor 25-27g/L；
C, after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, in 32-
Then shaking flask culture 15-20h at 35 DEG C presses 3:1:2 are mixed to get shake-flask seed liquid E；Wherein Shake flask medium is glucose sugar 32-
35g/L, beef peptone 13-15g/L, yeast extract 1.0-1.5g/L, sodium chloride 5-6g/L, potassium dihydrogen phosphate 0.5-0.8g/L,
PH7.0；
D, basal medium is configured, wherein molasses fermented alcohol effluent 700-750g/L, urea 5.0-6.0g/L, glucose are sugared
13-15g/L, surplus are water, and PH to 7 is adjusted with lye；The solid content of wherein molasses fermented alcohol effluent is 45%-
70%, the resting period is 3-5 months；
E, shake-flask seed liquid C, D, E are pressed 1:1:2 mixing are given birth to by inoculum concentration 5-7% access basic culture solution fermentations
The content for changing fulvic acid is the zymotic fluid of 25-28%；Wherein fermentation condition is：Mixing speed 270-300r/min, fermentation temperature
32-35 DEG C, fermentation period 55-65h.
Biochemical fulvic acid is a kind of organic acid, itself PH is 3.5-5.5, and it is PH that can reduce water body, improves water quality mistake
Hard situation；Meanwhile rich in humic acid, amino acid, organic calcium, the disease resistance of flower root system can be enhanced, and to two
Valence iron, zinc, manganese, copper have certain sequestering power, can promote effective absorption of the root system to nutrient, additionally it is possible to stablize water body and disappear
The beneficial bacteria of change system.
By molasses alcohol fermentation waste liquid it is waste biochemical fulvic acid in the present invention, itself contains a large amount of organic
Matter is not necessarily to processing and can be directly used for configuration medicine fertilizer after fermentation.
Illustrate the preparation method of the liquid medicine fertilizer provided by the present invention for hydroponic flowers below by specific embodiment.
Embodiment one
Prepare biochemical fulvic acid
A, aspergillus niger is activated on slant medium PDA, then the shaking flask culture 50h at 25 DEG C, the uniform balling-up of mycelia,
It is spare to obtain shake-flask seed liquid C；Wherein, 10g/L containing glucose, wheat bran leaching liquor 25g/L in Shake flask medium；
B, geotrichum candidum is activated on slant medium PDA, then the shaking flask culture 40h at 27 DEG C, obtains shake-flask seed
Liquid D；Wherein, Shake flask medium 10g/L containing glucose, wheat bran leaching liquor 25g/L；
C, after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, at 32 DEG C
Then lower shaking flask culture 20h presses 3:1:2 are mixed to get shake-flask seed liquid E；The 35g/L of sugar containing glucose, ox wherein in Shake flask medium
Meat peptone 15g/L, yeast extract 1.5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.7g/L, PH7.0；
D, basal medium is configured, wherein molasses fermented alcohol effluent 750g/L, urea 5.0g/L, glucose sugar 15g/L, remaining
Amount is water, and PH to 7 is adjusted with lye；The solid content of wherein molasses fermented alcohol effluent is 50%, and the resting period is 3
Month；
E, shake-flask seed liquid C, D, E are pressed 1:1:2 mixing access basic culture solution, low whipping speed by inoculum concentration 5%
270r/min, ferment under the conditions of 35 DEG C of temperature 60h, obtains the zymotic fluid that the content of biochemical fulvic acid is 26.7%, spare.
Configure medicine fertilizer
(1) human or animal's excrement takes zymotic fluid, is filtered by vacuum and is filtered after precipitation through anaerobic fermentation at 40 DEG C
Liquid concentrates 7 times using reverse osmosis membrane to filtrate, obtains concentrate.
(2) a certain amount of concentrate is taken, urea 196g, potassium dihydrogen phosphate 283g are added thereto, stirring and dissolving obtains solution
A；
(3) a certain amount of concentrate is taken, 6.6g FeSO are added thereto4·7H2O、0.52g ZnSO4·7H2O、
0.08gCuSO4·5H2O、0.20gMnSO4·4H2O, 20gHEEDTA, standing after stirring and dissolving makes HEEDTA be filled with metallic element
Divide chelating, obtains solution B；
(4) solution A and solution B are mixed, adds the zymotic fluid 113g obtained containing biochemical fulvic acid, stirring is equal
It is even, it is eventually adding ascorbic acid and adjusts PH to 6.8, obtain finished medicines fertilizer.
Embodiment two
Prepare biochemical fulvic acid
A, aspergillus niger is activated on slant medium PDA, then the shaking flask culture 70h at 20 DEG C, the uniform balling-up of mycelia,
It is spare to obtain shake-flask seed liquid C；Wherein, Shake flask medium 8g/L containing glucose, wheat bran leaching liquor 26g/L；
B, geotrichum candidum is activated on slant medium PDA, then the shaking flask culture 55h at 35 DEG C, obtains shake-flask seed
Liquid D；Wherein, Shake flask medium 8g/L containing glucose, wheat bran leaching liquor 26g/L；
C, after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, at 35 DEG C
Then lower shaking flask culture 16h presses 3:1:2 are mixed to get shake-flask seed liquid E；Wherein Shake flask medium sugar containing glucose 34g/L, beef
Peptone 14g/L, yeast extract 1.3g/L, sodium chloride 5.5g/L, potassium dihydrogen phosphate 0.7g/L, PH7.0；
D, basal medium is configured, wherein molasses fermented alcohol effluent 740g/L, urea 6.0g/L, glucose sugar 13g/L, remaining
Amount is water, and PH to 7 is adjusted with lye；The solid content of wherein molasses fermented alcohol effluent is 45%, and the resting period is 4
Month；
E, shake-flask seed liquid C, D, E are pressed 1:1:2 mixing access basic culture solution, low whipping speed by inoculum concentration 7%
Ferment 65h at 300r/min, 32 DEG C of temperature, obtains the zymotic fluid that the content of biochemical fulvic acid is 25.1%, spare.
Configure medicine fertilizer
(1) human or animal's excrement takes zymotic fluid, is filtered by vacuum and is filtered after precipitation through anaerobic fermentation at 30 DEG C
Liquid concentrates 10 times using reverse osmosis membrane to filtrate, obtains concentrate.
(2) a certain amount of concentrate is taken, urea 238g, potassium dihydrogen phosphate 308g are added thereto, stirring and dissolving obtains solution
A；
(3) a certain amount of concentrate is taken, 7.0g FeSO are added thereto4·7H2O、0.60g ZnSO4·7H2O、
0.04gCuSO4·5H2O、0.12gMnSO4·4H2O, 18gHEEDTA, standing after stirring and dissolving makes HEEDTA be filled with metallic element
Divide chelating, obtains solution B；
(4) solution A and solution B are mixed, adds the zymotic fluid 131.5g obtained containing biochemical fulvic acid, stirring is equal
It is even, it is eventually adding ascorbic acid and adjusts PH to 6.1, obtain finished medicines fertilizer.
Embodiment three
Prepare biochemical fulvic acid
A, aspergillus niger is activated on slant medium PDA, then the shaking flask culture 55h at 25 DEG C, the uniform balling-up of mycelia,
It is spare to obtain shake-flask seed liquid C；Wherein, 9g/L containing glucose, wheat bran leaching liquor 26g/L in Shake flask medium；
B, geotrichum candidum is activated on slant medium PDA, then the shaking flask culture 45h at 33 DEG C, obtains shake-flask seed
Liquid D；Wherein, Shake flask medium 9g/L containing glucose, wheat bran leaching liquor 26g/L；
C, after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, at 32 DEG C
Then lower shaking flask culture 18h presses 3:1:2 are mixed to get shake-flask seed liquid E；The 35g/L of sugar containing glucose, ox wherein in Shake flask medium
Meat peptone 15g/L, yeast extract 1.5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.7g/L, PH7.0；
D, basic culture solution is configured, wherein molasses fermented alcohol effluent 750g/L, urea 5.5g/L, glucose sugar 15g/L, remaining
Amount is water, and PH to 7 is adjusted with lye；The solid content of wherein molasses fermented alcohol effluent is 50%, and the resting period is 3
Month；
E, shake-flask seed liquid C, D, E are pressed 1:1:2 mixing access basic culture solution, low whipping speed by inoculum concentration 6%
290r/min, ferment under the conditions of 34 DEG C of temperature 58h, obtains the zymotic fluid that the content of biochemical fulvic acid is 27.8%, spare.
Configure medicine fertilizer
(1) human or animal's excrement takes zymotic fluid, is filtered by vacuum and is filtered after precipitation through anaerobic fermentation at 40 DEG C
Liquid concentrates 8 times using reverse osmosis membrane to filtrate, obtains concentrate.
(2) a certain amount of concentrate is taken, urea 210g, potassium dihydrogen phosphate 290g are added thereto, stirring and dissolving obtains solution
A；
(3) a certain amount of concentrate is taken, 7.0g FeSO are added thereto4·7H2O、0.70g ZnSO4·7H2O、
0.06gCuSO4·5H2O、0.28gMnSO4·4H2O, 21gHEEDTA, standing after stirring and dissolving makes HEEDTA be filled with metallic element
Divide chelating, obtains solution B；
(4) solution A and solution B are mixed, adds the zymotic fluid 125.9g obtained containing biochemical fulvic acid, stirring is equal
It is even, it is eventually adding ascorbic acid and adjusts PH to 6.4, obtain finished medicines fertilizer.
Technical solution of the present invention is exemplarily described invention above in conjunction with specific embodiment, it is clear that the present invention has
Body realization is not subject to the restrictions described above, as long as using the various non-realities of inventive concept and technical scheme of the present invention progress
Matter is improved, or it is not improved the design of invention and technical solution are directly applied into other occasions, the equal guarantors in the present invention
Within the scope of shield.
Claims (9)
1. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers, which is characterized in that include the following steps：
(1) it takes zymotic fluid, vacuum filtration to obtain filtrate through anaerobic fermentation in human or animal's excrement, reverse osmosis membrane is carried out to filtrate
7-10 times of concentration, obtains concentrate；
(2) a certain amount of concentrate is taken, nitrogenous fertilizer is added thereto and phosphate fertilizer, stirring and dissolving obtain solution A；
(3) a certain amount of concentrate is taken, metallic element fertilizer and HEEDTA are added thereto, is stood after stirring and dissolving, obtains solution
B；
(4) solution A and solution B are mixed, add biochemical fulvic acid, stir evenly, be eventually adding ascorbic acid adjust PH to
6-6.8 obtains finished medicines fertilizer；
Wherein, often configuration 1L liquid medicine is fertile, and the addition of the N element is 90-110g, the addition of the P elements is 50-
55g。
2. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers according to claim 1, which is characterized in that described
Metallic element fertilizer includes Fe elements, Zn elements, Cu elements, Mn elements.
3. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers according to claim 2, which is characterized in that often match
Set 1L liquid medicine fertilizer, the additive amounts of the Fe elements is that the additive amount of 1.30-1.48g, Zn element is 0.14-0.20g, Cu element
Additive amount be 0.01-0.02g, Mn element additive amount be 0.03-0.07g, the additive amount of the HEEDTA is 18-21g, described
The additive amount of biochemical fulvic acid is 30-35g.
4. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers according to claim 1, which is characterized in that described
Nitrogenous fertilizer is urea, the phosphate fertilizer is dipotassium hydrogen phosphate.
5. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers according to claim 1, which is characterized in that described
The preparation method of biochemical fulvic acid is as follows：
A, aspergillus niger is activated on slant medium PDA, then shaking flask culture 2-3 days at 20-25 DEG C, mycelia uniformly at
It is spare to obtain shake-flask seed liquid C for ball；Wherein, Shake flask medium is glucose 8-10g/L, wheat bran leaching liquor 25-27g/L；
B, geotrichum candidum is activated on slant medium PDA, then shaking flask culture 2-3 days at 25-27 DEG C, obtain shake-flask seed
Liquid D；Wherein, Shake flask medium is glucose 8-10g/L, wheat bran leaching liquor 25-27g/L；
C, after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, at 32-35 DEG C
Then lower shaking flask culture 15-20h presses 3:1:2 are mixed to get shake-flask seed liquid E；Wherein Shake flask medium is glucose sugar 32-35g/
L, beef peptone 13-15g/L, yeast extract 1.0-1.5g/L, sodium chloride 5-6g/L, potassium dihydrogen phosphate 0.5-0.8g/L,
PH7.0；
D, basal medium is configured, wherein molasses fermented alcohol effluent 700-750g/L, urea 5.0g/L, glucose sugar 13-15g/
L, surplus are water, and PH to 7 is adjusted with lye；
E, shake-flask seed liquid C, D, E are pressed 1:1:2 mixing obtain biochemical Huang by inoculum concentration 5-7% access basic culture solution fermentations
The zymotic fluid that the content of rotten acid is 25-28%.
6. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers according to claim 5, which is characterized in that described
The solid content of molasses fermented alcohol effluent in step d is 45%-70%.
7. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers according to claim 5 or 6, which is characterized in that
The resting period of the molasses fermented alcohol effluent is 3-5 months.
8. a kind of preparation method of liquid medicine fertilizer for hydroponic flowers according to claim 5, which is characterized in that described
Fermentation condition is in step e：Mixing speed 270-300r/min, 32-35 DEG C of fermentation temperature, fermentation period 55-65h.
9. a kind of liquid medicine fertilizer for hydroponic flowers uses claim 1-8 any one of them one kind to be spent for water planting
The preparation method of the liquid medicine fertilizer of grass is prepared.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949833A (en) * | 2018-06-27 | 2018-12-07 | 安徽瑞然生物药肥科技有限公司 | A kind of preparation method for the fulvic acid being directly used in crops medicine fertilizer additive |
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