CN108949833A - A kind of preparation method for the fulvic acid being directly used in crops medicine fertilizer additive - Google Patents
A kind of preparation method for the fulvic acid being directly used in crops medicine fertilizer additive Download PDFInfo
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- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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Abstract
The invention discloses a kind of preparation methods of fulvic acid for being directly used in crops medicine fertilizer additive, comprising the following steps: (1) after activating aspergillus niger, it is spare to obtain shake-flask seed liquid C for shaking flask culture 2-3 days, the uniform balling-up of mycelia；(2), after activating geotrichum candidum, shaking flask culture 2-3 days obtains shake-flask seed liquid D；(3), respectively by after the activation of bacillus pumilus, Penicillium notatum and Pseudomonas alba, then shaking flask culture 15-20h is mixed to get shake-flask seed liquid E by 3:1:2；(4), basal medium is configured, wherein molasses fermented alcohol effluent 700-750g/L, urea 5.0g/L, glucose sugar 13-15g/L, surplus is water, adjusts PH to 7 with lye；(5), shake-flask seed liquid C, D, E are mixed by 1:1:2, by inoculum concentration 5-7% access basal medium fermentation, the content for obtaining fulvic acid is the fermentation liquid of 25-28%.It is an advantage of the current invention that not using chemical reagent, the activity of fulvic acid is not destroyed.
Description
Technical field
The invention belongs to agricultural fulvic acid preparation technical fields, and in particular to one kind is directly used in crops medicine fertilizer additive
Fulvic acid preparation method.
Background technique
Fulvic acid is molecular dimension minimum in humic acid, the maximum component of activity, is the essence in humic acid effective component,
It is easily by bio-absorbable.Fulvic acid is added in fertilizer, the disease-resistant energy that there is the hardness for such as reducing water, enhance plant
Power promotes plant to all multiactions such as the absorption of available nutrient of fertilizer, therefore its preparation for being widely used in biological medicine fertilizer, preparation.
The fulvic acid produced both at home and abroad at present is mostly mineral fulvic acid, i.e., extracts from weathered coal, lignite, peat, one
As extracting method be alkali-soluble acid analysis method, strong acid extraction process, sulfuric acid Ethanol Method, ion-exchange-resin process.Although mineral fulvic acid mentions
Low in raw material price is taken, but resource is non-renewable, and extraction process is relative complex, ring need to be caused with a large amount of chemical reagent are used
Border pollution, goes back the active group of destructible fulvic acid, reduces bioactivity.
Summary of the invention
Technical problem to be solved by the present invention lies in: mineral fulvic acid extraction process is complicated, using a large amount of chemical reagent,
Environment easy to pollute influences the bioactivity of fulvic acid.
The present invention solves above-mentioned technical problem using following technical scheme:
A kind of preparation method for the fulvic acid being directly used in crops medicine fertilizer additive, comprising the following steps:
(1) aspergillus niger is activated on slant medium PDA, then shaking flask culture 2-3 days at 20-25 DEG C, mycelia is equal
It is spare to obtain shake-flask seed liquid C for even balling-up；
(2), geotrichum candidum is activated on slant medium PDA, then shaking flask culture 2-3 days at 25-27 DEG C, are shaken
Bottle seed liquor D；
(3), after bacillus pumilus, Penicillium notatum and Pseudomonas alba being activated on slant medium NBY respectively,
Shaking flask culture 15-20h at 32-35 DEG C, is then mixed to get shake-flask seed liquid E by 3:1:2；
(4), basal medium is configured, wherein molasses fermented alcohol effluent 700-750g/L, urea 5.0g/L, glucose are sugared
13-15g/L, surplus are water, adjust PH to 7 with lye；
(5), shake-flask seed liquid C, D, E are mixed by 1:1:2, by inoculum concentration 5-7% access basal medium fermentation, is obtained
The content of fulvic acid is the fermentation liquid of 25-28%.
Preferably, the preparation method of a kind of fulvic acid being directly used in crops medicine fertilizer additive of the present invention, institute
The solid content for stating the molasses fermented alcohol effluent in step (4) is 45%-70%.
Preferably, the preparation method of a kind of fulvic acid being directly used in crops medicine fertilizer additive of the present invention, institute
The resting period for stating molasses fermented alcohol effluent is 3-5 months.Molasses fermented alcohol effluent storage period, carries out spontaneous fermentation, thus
Influence the content of final fulvic acid.
Preferably, the preparation method of a kind of fulvic acid being directly used in crops medicine fertilizer additive of the present invention, institute
The resting period for stating molasses fermented alcohol effluent is 4 months.
Preferably, the preparation method of a kind of fulvic acid being directly used in crops medicine fertilizer additive of the present invention, institute
It states and contains glucose 8-10g/L, wheat bran leaching liquor 25- in the culture medium of shaking flask culture described in step (1) and step (2)
27g/L。
Preferably, the preparation method of a kind of fulvic acid being directly used in crops medicine fertilizer additive of the present invention, institute
State in step (3) 32-35g/L of sugar containing glucose, beef peptone 13-15g/L, yeast extract 1.0- in the culture medium of shaking flask culture
1.5g/L, sodium chloride 5-6g/L, potassium dihydrogen phosphate 0.5-0.8g/L, PH7.0.
Preferably, the preparation method of a kind of fulvic acid being directly used in crops medicine fertilizer additive of the present invention, institute
State fermentation condition in step (5) are as follows: mixing speed 270-300r/min, 32-35 DEG C of fermentation temperature, fermentation period 55-65h.
The technology of the present invention the utility model has the advantages that
Technical solution of the present invention using the molasses fermented alcohol effluent rich in organic matter as raw material, sent out by microbe inoculation
Ferment can be directly used for the configuration of biological medicine fertilizer without carrying out any purification processes i.e., not use chemical reagent, environmental-friendly, and
The fulvic acid activity of acquisition is higher；Using molasses fermented alcohol effluent as raw material, turn waste into wealth, is further conducive to environmental protection.
Specific embodiment
For convenient for those skilled in the art understand that technical solution of the present invention, now in conjunction with specific embodiment to the technology of the present invention side
Case is described further.
Embodiment one
(1) aspergillus niger is activated on slant medium PDA, then the shaking flask culture 50h at 25 DEG C, mycelia uniformly at
It is spare to obtain shake-flask seed liquid C for ball；Wherein, 10g/L containing glucose, wheat bran leaching liquor 25g/L in Shake flask medium；
(2) geotrichum candidum is activated on slant medium PDA, then the shaking flask culture 40h at 27 DEG C, obtains shake-flask seed
Liquid D；Wherein, Shake flask medium 10g/L containing glucose, wheat bran leaching liquor 25g/L；
(3) after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, 32
Shaking flask culture 20h at DEG C, is then mixed to get shake-flask seed liquid E by 3:1:2；Wherein the 35g/L of sugar containing glucose in Shake flask medium,
Beef peptone 15g/L, yeast extract 1.5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.7g/L, PH7.0；
(4) basal medium is configured, wherein molasses fermented alcohol effluent 750g/L, urea 5.0g/L, glucose sugar 15g/L,
Surplus is water, adjusts PH to 7 with lye；Wherein the solid content of molasses fermented alcohol effluent is 50%, and the resting period is 3
Month；
(5) shake-flask seed liquid C, D, E are mixed by 1:1:2, accesses basal medium, low whipping speed by inoculum concentration 5%
270r/min, ferment under the conditions of 35 DEG C of temperature 60h, obtains the fermentation liquid that the content of fulvic acid is 26.7%.
Embodiment two
(1) aspergillus niger is activated on slant medium PDA, then the shaking flask culture 70h at 20 DEG C, mycelia uniformly at
It is spare to obtain shake-flask seed liquid C for ball；Wherein, Shake flask medium 8g/L containing glucose, wheat bran leaching liquor 26g/L；
(2) geotrichum candidum is activated on slant medium PDA, then the shaking flask culture 55h at 35 DEG C, obtains shake-flask seed
Liquid D；Wherein, Shake flask medium 8g/L containing glucose, wheat bran leaching liquor 26g/L；
(3) after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, 35
Shaking flask culture 16h at DEG C, is then mixed to get shake-flask seed liquid E by 3:1:2；Wherein Shake flask medium sugar containing glucose 34g/L, ox
Meat peptone 14g/L, yeast extract 1.3g/L, sodium chloride 5.5g/L, potassium dihydrogen phosphate 0.7g/L, PH7.0；
(4) basal medium is configured, wherein molasses fermented alcohol effluent 740g/L, urea 6.0g/L, glucose sugar 13g/L,
Surplus is water, adjusts PH to 7 with lye；Wherein the solid content of molasses fermented alcohol effluent is 45%, and the resting period is 4
Month；
(5) shake-flask seed liquid C, D, E are mixed by 1:1:2, accesses basal medium, low whipping speed by inoculum concentration 7%
300r/min, ferment at 32 DEG C of temperature 65h, obtains the fermentation liquid that the content of fulvic acid is 25.1%.
Embodiment three
(1) aspergillus niger is activated on slant medium PDA, then the shaking flask culture 55h at 25 DEG C, mycelia uniformly at
It is spare to obtain shake-flask seed liquid C for ball；Wherein, 9g/L containing glucose, wheat bran leaching liquor 26g/L in Shake flask medium；
(2) geotrichum candidum is activated on slant medium PDA, then the shaking flask culture 45h at 33 DEG C, obtains shake-flask seed
Liquid D；Wherein, Shake flask medium 9g/L containing glucose, wheat bran leaching liquor 26g/L；
(3) after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, 32
Shaking flask culture 18h at DEG C, is then mixed to get shake-flask seed liquid E by 3:1:2；Wherein the 35g/L of sugar containing glucose in Shake flask medium,
Beef peptone 15g/L, yeast extract 1.5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.7g/L, PH7.0；
(4) basal medium is configured, wherein molasses fermented alcohol effluent 750g/L, urea 5.5g/L, glucose sugar 15g/L,
Surplus is water, adjusts PH to 7 with lye；Wherein the solid content of molasses fermented alcohol effluent is 50%, and the resting period is 3
Month；
(5) shake-flask seed liquid C, D, E are mixed by 1:1:2, accesses basal medium, low whipping speed by inoculum concentration 6%
290r/min, ferment under the conditions of 34 DEG C of temperature 58h, obtains the fermentation liquid that the content of fulvic acid is 27.8%.
Aspergillus niger, geotrichum candidum used in above-described embodiment, bacillus pumilus, Penicillium notatum and Pseudomonas alba strain
Be it is commercially available, as the prior art, have no direct relation with improvement of the invention, be not described in detail herein.
Technical solution of the present invention is exemplarily described invention above in conjunction with specific embodiment, it is clear that present invention tool
Body realization is not subject to the restrictions described above, as long as using the various non-realities that the inventive concept and technical scheme of the present invention carry out
Matter improve, or it is not improved the conception and technical scheme of invention are directly applied into other occasions, in guarantor of the invention
Within the scope of shield.
Claims (7)
1. a kind of preparation method for the fulvic acid for being directly used in crops medicine fertilizer additive, which comprises the following steps:
(1) aspergillus niger is activated on slant medium PDA, then shaking flask culture 2-3 days at 20-25 DEG C, mycelia uniformly at
It is spare to obtain shake-flask seed liquid C for ball；
(2), geotrichum candidum is activated on slant medium PDA, then shaking flask culture 2-3 days at 25-27 DEG C, obtain shaking flask kind
Sub- liquid D；
(3), after respectively activating bacillus pumilus, Penicillium notatum and Pseudomonas alba on slant medium NBY, in 32-35
Shaking flask culture 15-20h at DEG C, is then mixed to get shake-flask seed liquid E by 3:1:2；
(4), basal medium is configured, wherein molasses fermented alcohol effluent 700-750g/L, urea 5.0g/L, glucose sugar 13-
15g/L, surplus are water, adjust PH to 7 with lye；
(5), shake-flask seed liquid C, D, E are mixed by 1:1:2, by inoculum concentration 5-7% access basal medium fermentation, obtains yellow corruption
The content of acid is the fermentation liquid of 25-28%.
2. a kind of preparation method of fulvic acid for being directly used in crops medicine fertilizer additive according to claim 1, special
Sign is that the solid content of the molasses fermented alcohol effluent in the step (4) is 45%-70%.
3. a kind of preparation method of fulvic acid for being directly used in crops medicine fertilizer additive according to claim 1 or 2,
It is characterized in that, the resting period of the molasses fermented alcohol effluent is 3-5 months.
4. a kind of preparation method of fulvic acid for being directly used in crops medicine fertilizer additive according to claim 3, special
Sign is that the resting period of the molasses fermented alcohol effluent is 4 months.
5. a kind of preparation method of fulvic acid for being directly used in crops medicine fertilizer additive according to claim 1, special
Sign is, soaks in the culture medium of shaking flask culture described in the step (1) and step (2) containing glucose 8-10g/L, wheat bran
Extract 25-27g/L.
6. a kind of preparation method of fulvic acid for being directly used in crops medicine fertilizer additive according to claim 1, special
Sign is, the 32-35g/L of sugar containing glucose, beef peptone 13-15g/L, ferment in the culture medium of shaking flask culture in the step (3)
Female cream 1.0-1.5g/L, sodium chloride 5-6g/L, potassium dihydrogen phosphate 0.5-0.8g/L, PH7.0.
7. a kind of preparation method of fulvic acid for being directly used in crops medicine fertilizer additive according to claim 1, special
Sign is, fermentation condition in the step (5) are as follows: mixing speed 270-300r/min, 32-35 DEG C of fermentation temperature, and fermentation period
55-65h。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110818457A (en) * | 2019-12-03 | 2020-02-21 | 杭州三得农业科技有限公司 | Process for resolving macromolecular organic carbon and converting macromolecular organic carbon into fulvic acid by using sound barrier principle |
| CN111646851A (en) * | 2020-06-30 | 2020-09-11 | 蔡雨洵 | Yellow humic acid granular fertilizer and preparation method thereof |
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| CN101031203A (en) * | 2004-09-24 | 2007-09-05 | 生物耐克斯特公司 | Compositions for use against one or more pathogens |
| CN107557425A (en) * | 2017-10-26 | 2018-01-09 | 齐齐哈尔信泰农业科技有限公司 | The method that biochemical fulvic acid is produced with excrement of animals fermentation biogas slurry treatment maize straw |
| CN108658668A (en) * | 2018-06-27 | 2018-10-16 | 安徽瑞然生物药肥科技有限公司 | A kind of preparation method and medicine fertilizer of liquid medicine fertilizer for hydroponic flowers |
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2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101031203A (en) * | 2004-09-24 | 2007-09-05 | 生物耐克斯特公司 | Compositions for use against one or more pathogens |
| CN107557425A (en) * | 2017-10-26 | 2018-01-09 | 齐齐哈尔信泰农业科技有限公司 | The method that biochemical fulvic acid is produced with excrement of animals fermentation biogas slurry treatment maize straw |
| CN108658668A (en) * | 2018-06-27 | 2018-10-16 | 安徽瑞然生物药肥科技有限公司 | A kind of preparation method and medicine fertilizer of liquid medicine fertilizer for hydroponic flowers |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110818457A (en) * | 2019-12-03 | 2020-02-21 | 杭州三得农业科技有限公司 | Process for resolving macromolecular organic carbon and converting macromolecular organic carbon into fulvic acid by using sound barrier principle |
| CN111646851A (en) * | 2020-06-30 | 2020-09-11 | 蔡雨洵 | Yellow humic acid granular fertilizer and preparation method thereof |
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