CN108569935A - A kind of vegetables foliage bacterial fertilizer and the preparation method and application thereof - Google Patents
A kind of vegetables foliage bacterial fertilizer and the preparation method and application thereof Download PDFInfo
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- CN108569935A CN108569935A CN201810719778.6A CN201810719778A CN108569935A CN 108569935 A CN108569935 A CN 108569935A CN 201810719778 A CN201810719778 A CN 201810719778A CN 108569935 A CN108569935 A CN 108569935A
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- fertilizer
- vegetables
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- vegetable
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a kind of vegetables foliage bacterial fertilizers and the preparation method and application thereof, with 20 40 parts of Bacillus cercus microbial inoculum, 6 10 parts of chitosan, 8 12 parts of amino acid, fertile 10 15 parts micro-, 20 40 parts of water.Applied to increase the yield of vegetables, improve quality of vegetable, induction enhancing vegetable disease-resistant, promote vegetable growth etc..Have many advantages, such as significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease, improves yield and quality, is of low cost and easy to spread.
Description
Technical field
The present invention relates to vegetable growing technologies and fertilising field, and in particular to a kind of vegetables foliage bacterial fertilizer and preparation method thereof
With application.
Background technology
The nutriment of vegetables includes mainly minerals, vitamin, fiber etc., and the content of these substances is higher, vegetables
Nutritive value is also higher.It is estimated that there is more than 20 hundred million or more people to be caused a variety of diseases by environmental pollution in the world now,
How to solve the problems, such as to be increasingly subject to people's concern because environmental pollution generates a large amount of oxygen radicals.One of effective way of solution,
It is to increase antioxidant collaboration in food to remove excessive damaging active oxygen, active nitrogen.The study found that having perhaps in vegetables
Multivitamin, mineral trace element and relevant phytochemicals, enzyme etc. are all effective antioxidants, so vegetables are not
Only it is the health food of low sugar, less salt, low fat, while can also effectively mitigates damage of the environmental pollution to human body, while vegetables
Prevention effect also is played to various diseases.
Currently, a large amount of uses, poor efficiency and its high environmental risk brought of chemical fertilizer are always to perplex in vegetables production
The whole world significant problem, cause soil hardening, input increases, farmland pollution, agricultural product quality and safety decline etc. it is many after
Fruit has influenced the safety of victual and the sustainable development of vegetables industry.Foliage bacterial fertilizer is by being sprayed onto plant leaf surface
After can be absorbed by plant rapidly by plant leaf blade, avoid from nutrient when soil application easily by soil consolidation, root
Portion absorbs the problems such as slow, has many advantages, such as to absorb fast, nutrient loss less.Currently, there are many kinds of existing foliage bacterial fertilizers, carrying
Also there is certain effect in the yield of high crop, but to improve crop yield effect not significantly equal scarce enough for all different degrees of presence
It falls into and there are no the ideal foliage bacterial fertilizers designed exclusively for vegetables in the prior art.
Invention content
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, meets vegetables at significant effect
The vegetables foliage bacterial fertilizer of growing nutrient demand.
Technical scheme is as follows：
A kind of vegetables foliage bacterial fertilizer, it is made from the following raw materials in parts by weight：It is prepared from the following ingredients in percentage by group
At：20-40 parts of Bacillus cercus microbial inoculum, 6-10 parts of chitosan, 8-12 parts of amino acid, micro- 10-15 parts of fertilizer, 20-40 parts of water.
Preferably, it is made from the following raw materials in parts by weight：30 parts of Bacillus cercus microbial inoculum, 8 parts of chitosan, amino acid
10 parts, fertile 12 parts micro-, 40 parts of water.
Preferably, the strain contained in Bacillus cercus microbial inoculum is Bacillus cercus LY05 (CCTCC NO:M
2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08 (CCTCCNO:
M2015399 a combination of one or more).
Wherein, Bacillus cercus microbial inoculum preparation includes the following steps：(1) by bacillus cereus strain LY05, LGY06
Or the inclined-planes WL08 preserve test tube seed, are seeded in minimal medium respectively, shaken cultivation to exponential phase, obtain bacterium
Liquid；(2) bacterium solution of acquisition is seeded to the seed fermentation containing minimal medium with the inoculum concentration of 10% percent by volume
In tank, culture to exponential phase obtains seed liquor；(3) seed liquor of acquisition is connect according to the inoculum concentration of 10% volume ratio
Kind enter fermentation medium in, fermented and cultured 2-3 days at 30-35 DEG C, 150-200rpm, acquisition zymotic fluid, zymotic fluid it is effective
Viable count >=5,000,000,000/mL, zymotic fluid directly dilute 5-20 times as single bacterial strain liquid bacterial agent；(4) by wax-like gemma bar
The single bacterial strain liquid bacterial agent of bacteria strain LY05, LGY06 or WL08 are mixed final by a combination of one or more
Bacillus cercus mix bacterium agent.
Further, the chitosan is water solubility, and deacetylation is more than 95%；The amino acid is proline, sweet ammonia
Acid, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine,
Arbitrary five kinds or more of combination in valine, alanine, serine, arginine, asparatate, cysteine；It is described
Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium of 0.5-1
The mixed fertilizer of+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer；The water is sterile water.
The preparation method of the vegetables foliage bacterial fertilizer of the present invention, comprises the technical steps that：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportioning, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportioning by regulation proportioning；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH6.0-6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to vegetables bacterial fertilizer products.
The bio-bacterial manure of the present invention can be applied in following (1)-(4) are at least one：
(1) increase the yield of vegetables；(2) improve quality of vegetable；(3) induction enhancing vegetable disease-resistant, effective prevention and control vegetables
The diseases such as bacterial wilt, damping-off, shot hole；(4) promote the growth of vegetable plant strain.
Signified vegetables include leaf vegetables, melon-fruit-like vegetable, stem vegetables, flower vegetables, root vegetable etc..
Compared with prior art, the present invention foliage bacterial fertilizer of the present invention using Bacillus cercus microbial inoculum, plant growth-promoting substance,
The compositions such as a great number of elements and trace element can effectively improve vegetables economic benefit, nutrient and flavour quality, and plant is promoted well to give birth to
It is long, and vegetable disease-resistant can effectively be induced to enhance, have significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease,
Improve yield and quality, it is of low cost and easy to spread the advantages that.
Specific implementation mode
With reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1：
1. bio-bacterial manure is formed and is prepared：
Bio-bacterial manure A formula compositions and preparation, (1) composition：30 parts of Bacillus cercus microbial inoculum, 8 parts of chitosan, amino acid
10 parts, fertile 12 parts micro-, 40 parts of water.(2) it prepares：1. Bacillus cercus microbial inoculum is taken by regulation proportioning, it is spare；2. being matched by regulation
It takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred to be dissolved in the required sterile water matched；3. 1. microbial inoculum that step is obtained is added
In 2. solution that step obtains, under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, it is sufficiently mixed in sterile blender
It is even, it is packed up to vegetables foliage bacterial fertilizer A.
Foliar fertilizer B formula compositions and preparation, (1) composition：8 parts of chitosan, 0 part of amino acid 1, fertile 12 parts micro-, 70 parts of water.
(2) it prepares：1. taking chitosan, amino acid and micro- fertilizer to be sufficiently stirred by regulation proportioning to be dissolved in the required sterile water matched, be made
Vegetable leaf leaven B.
2. field trial：
Experimental field overview：Experimental field it is located at Guizhou Xifeng County Western Hills township herbary, kind is tomato " drawing 118 in red pool ".
It is yellow earth for examination soil types, random in vegetable garden before experiment, multiple spot mixing acquisition depth 0-60cm soil sample is as Soil Background
Value, pH value 5.86, total nitrogen content 1.74gkg-1, content of organic matter 39.53gkg-1, alkaline hydrolysis nitrogen content is
108.76mg·kg-1, available phosphorus content 47.63mgkg-1, effective potassium content is 103.47mgkg-1。
Field experimental design：RANDOMIZED BLOCK DESIGN is taken in experiment, using complete stool (including blade face positive and negative, limb, fruit
Deng) spraying, if 100-200 times of liquid of foliage bacterial fertilizer, 100 times of liquid of foliar fertilizer, spray clear water are control (CK), totally 3 processing repeat
4 times, totally 12 cells, around set protection row.Administration time be seedling stage, growth medium, it is prosperous apply respectively for a long time, amount of application distinguish
For 30,45,60kg/ mus.Weather is based on fine during experiment, or it is cloudy, spray in latter 3-4 days without rainfall.
3. analysis project and method：
Growth, yield and quality investigation：Investigate plant height, expansion degree, stem thickness, yield, quality survey dry matter, vitamin C, can
Titrable acid, soluble solid, lycopene.Investigate breeding time tomato bacterial wilt, root rot, damping-off, root-knot nematode, aphid
A situation arises for worm, counts cumulative life-incidence or insect population number；Incidence (%)=100 × (morbidity strain number/investigation strain number).
4. embodiment effect：
From table 1 to table 3 as can be seen that using vegetables special bio bacterial manure can be effectively facilitated tomato plant growth,
Increase per mu yield, significantly improve fruit dry matter, vitamin C and soluble solid (SSC) content, reduces titratable acid (TA)
Content promotes the formation of yield of vegetables and the improvement of quality；And it can effectively reduce tomato bacterial wilt, damping-off, shot hole hair
Raw harm.
The application foliage bacterial fertilizer of table 1 is grown to tomato and the influence of yield
Note：With marking different lowercase letters after column data in P<0.05 time difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 time difference is same up to pole level of signifiance following tables
Influence of the application foliage bacterial fertilizer of table 2 to tomato quality
A situation arises to tomato pest and disease damage for the application of table 3 foliage bacterial fertilizer
The specific application example of the above only present invention, the present invention also has other embodiments, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection domain of the presently claimed invention.
Claims (7)
1. a kind of vegetables foliage bacterial fertilizer, it is characterised in that：It is made from the following raw materials in parts by weight：Bacillus cercus microbial inoculum
20-40 parts, 6-10 parts of chitosan, 8-12 parts of amino acid, micro- 10-15 parts of fertilizer, 20-40 parts of water.
2. vegetables foliage bacterial fertilizer according to claim 1, it is characterised in that：It is made from the following raw materials in parts by weight：Wax
30 parts of shape gemma bacillus agent, 8 parts of chitosan, 0 part of amino acid 1, fertile 12 parts micro-, 40 parts of water.
3. vegetables foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：Contain in said waxy gemma bacillus agent
Some strains are Bacillus cercus LY05 (CCTCC NO:M 2015397), Bacillus cercus LGY06 (CCTCC NO:M
And Bacillus cercus WL08 (CCTCCNO 2015398):M2015399 a combination of one or more).
4. vegetables foliage bacterial fertilizer according to claim 3, it is characterised in that：It is prepared by said waxy gemma bacillus agent
Following steps：(1) inclined-planes bacillus cereus strain LY05, LGY06 or WL08 are preserved into test tube seed, be seeded to respectively inorganic
In salt culture medium, shaken cultivation to exponential phase obtains bacterium solution；(2) by the bacterium solution of acquisition with 10% percent by volume
Inoculum concentration is seeded in the seed fermentation tank containing minimal medium, and culture to exponential phase obtains seed liquor；(3) will
The seed liquor of acquisition is inoculated with according to the inoculum concentration of 10% volume ratio in fermentation medium, is issued in 3035 DEG C, 150200rpm
Ferment culture 2-3d, obtains zymotic fluid, the living bacteria count of zymotic fluid >=5,000,000,000/mL, zymotic fluid, which is directly diluted 5-20 times, is
For single bacterial strain liquid bacterial agent；(4) the single bacterial strain liquid bacterial agent of bacillus cereus strain LY05, LGY06 or WL08 are pressed
A combination of one or more is mixed and made into final Bacillus cercus mix bacterium agent.
5. vegetables foliage bacterial fertilizer according to claim 1 or 2, it is characterised in that：The chitosan is water solubility, deacetylated
Degree is more than 95%；The amino acid be proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, lysine,
Threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate, half
Arbitrary five kinds or more of combination in cystine；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:
0.5-1:0.5-1:The mixed fertilizer of potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1；It is described
Water is sterile water.
6. a kind of preparation method of vegetables foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that：Including following technique
Step：
Step 1. takes claim 4 said waxy gemma bacillus agent by regulation proportioning, spare；
Step 2. takes chitosan, amino acid and micro- fertilizer to be sufficiently stirred in the sterile water for being dissolved in required proportioning by regulation proportioning；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, the item of pH6.0-6.8
Under part, it is sufficiently mixed in sterile blender uniformly, it is packed up to vegetables bacterial fertilizer products.
7. a kind of application of vegetables foliage bacterial fertilizer as claimed in claim 1 or 2, it is characterised in that including the following aspects
Using：(1) increase the yield of vegetables；(2) improve quality of vegetable；(3) induction enhancing vegetable disease-resistant, effective prevention and control vegetables blueness are withered
The diseases such as disease, damping-off, shot hole；(4) promote the growth of vegetable plant strain；
Signified vegetables include leaf vegetables, melon-fruit-like vegetable, stem vegetables, flower vegetables, root vegetable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810719778.6A CN108569935A (en) | 2018-06-30 | 2018-06-30 | A kind of vegetables foliage bacterial fertilizer and the preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810719778.6A CN108569935A (en) | 2018-06-30 | 2018-06-30 | A kind of vegetables foliage bacterial fertilizer and the preparation method and application thereof |
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| Publication Number | Publication Date |
|---|---|
| CN108569935A true CN108569935A (en) | 2018-09-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810719778.6A Withdrawn CN108569935A (en) | 2018-06-30 | 2018-06-30 | A kind of vegetables foliage bacterial fertilizer and the preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114478109A (en) * | 2021-12-27 | 2022-05-13 | 云南云叶化肥股份有限公司 | Biological foliar fertilizer and preparation method thereof |
-
2018
- 2018-06-30 CN CN201810719778.6A patent/CN108569935A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114478109A (en) * | 2021-12-27 | 2022-05-13 | 云南云叶化肥股份有限公司 | Biological foliar fertilizer and preparation method thereof |
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Application publication date: 20180925 |