CN108486070A - 除草剂抗性突变体及其应用 - Google Patents

除草剂抗性突变体及其应用 Download PDF

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CN108486070A
CN108486070A CN201810138888.3A CN201810138888A CN108486070A CN 108486070 A CN108486070 A CN 108486070A CN 201810138888 A CN201810138888 A CN 201810138888A CN 108486070 A CN108486070 A CN 108486070A
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唐晓艳
邓兴旺
周君莉
陈竹锋
王承旭
金名捺
谢刚
李早霞
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Shenzhen Jie Tian Model Biology Technology Co Ltd
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Abstract

本发明公开了一种除草剂抗性突变体及其应用，属于植物生物技术领域，具体而言，本发明涉及一种能使植物产生除草剂抗性的蛋白核酸突变体序列及该除草剂抗性突变体其在植物育种中的应用。本发明用烯草酮除草剂在水稻突变体库中发现了六个具有ACCase抑制剂类除草剂抗性的突变体，所述抗性突变体可以耐受ACCase抑制剂类除草剂，尤其是烯草酮。本发明还开发了具有ACCase抑制剂类除草剂抗性的蛋白及其编码基因在转基因或者非转基因植物育种中的应用，所述除草剂抗性基因和蛋白可用于培育具有除草剂抗性的植物，尤其是禾本科农作物，如水稻、玉米、小麦、大麦、高粱、黍、谷子等，在植物育种和农业生产中具有重大的应用价值。

Description

除草剂抗性突变体及其应用
技术领域
本发明属于植物生物技术领域，具体而言，本发明涉及一种能使植物产生除草剂抗性的蛋白核酸突变体序列及该除草剂抗性突变体其在植物育种中的应用。
技术背景
随着水稻直播栽培技术在农业生产中的大面积应用，水稻直播生产中农田杂草问题成为影响农作物产量的重要因素。一方面，人工除草所需要消耗的人力资源和成本很高，而且不便于农业集约化生产，严重制约了农作物种植向高产、优质和低成本方向的发展进程。另一方面，水稻田中的禾本科杂草存在着防治难的问题，能够用于杀除禾本科杂草的除草剂通常也能够杀死水稻，因此除草剂的使用在时间和空间上受到了极大限制，如需要在农作物播种前一段时间使用除草剂。
除草剂作为现代农业生产体系的重要组成部分，是农田除草技术中最可靠且最经济的手段。自2，4-D于20世纪四十年代开始使用以来，除草剂工业已有60多年的发展历史，迄今已成功开发出了一大批选择性除草剂。其中乙酰辅酶A羧化酶(Acetyl-CoACarboxylase，[EC6.4.1.2]，ACCase)是化学除草剂的一个重要靶标。ACCase发现于1958年，是生物素包含酶，它在生物体内催化乙酰辅酶A羧化形成丙二酰辅酶A，为脂肪酸和许多次生代谢产物的合成提供底物，是脂肪酸生物合成的关键酶或限速酶，在水稻中，ACCase基因位于水稻第五号染色体上(Genebank Accession NO：AP014961.1，基因区域：13063871-13078401)。ACCase抑制剂以ACCase为作用靶标，抑制禾本科植物体内的脂肪酸合成，选择性高，在植物体内传导，能够苗后防除一年或多年生禾本科杂草。它具有高效、低毒、施用期长、对后茬作物安全等优点，因而在除草剂中占有重要地位(姜莉莉，史晓斌.ACCase抑制剂类除草剂的作用机理[J].农药研究与应用，2010 14(4)：14-17)。
ACCase抑制除草剂分为3类，APP(芳氧苯氧丙酸)类、CHD(环已烯酮)类以及DEN(苯基吡唑啉)类，其中APP类包括精喹禾灵(quizalofop-e-ethyl)，禾草灵(diclofop-methyl)，炔草酯(clodinafop-propargyl)等主要品种；CHD类包括肟草酮(tralkoxydim)，噻草酮(cycloxdim)，稀禾啶(sethoxydim)，烯草酮(clethodim)，禾草灭(alloxydim)等主要品种；DEN类主要代表药剂有唑啉草酯(pinoxaden)。烯草酮(clethodim)其中文商品名为收乐通、赛乐特，化学名2-{1-[(3-氯-2-烯丙基)氧]亚胺基丙基}-5-[2-(乙硫基)丙基]-3-羟基-2-环己烯-1-酮，CAS号：99129-21-2，是由Kincade RT等在1987年英国作物保护会议上介绍的一种新的具有优良选择性的除草剂。其作用机制与稀禾啶、精吡氟禾草灵、精喹禾灵等相同，均为乙酰辅酶A羧化酶(ACCase)抑制剂，主要用来防除大豆、棉花、亚麻、向日葵、苜蓿、花生、油菜、甜菜、烟草、马铃薯等阔叶作物田以及蔬菜和果树中的多种一年生和多年生禾本科杂草，对阔叶作物高度安全。该药为内吸传导型除草剂，施药后经杂草叶片吸收，在植物体内迅速传导至根系及分生组织，最终造成细胞坏死，植株死亡。其在土壤中易分解，土壤活性小，持效期短，对后茬作物无影响(杨玉廷，高爽，张宗俭等.除草剂烯草酮的应用技术研究[J].农药，2005 44(4)：186–189)。
目前我国主要使用酰胺类和磺酰脲类除草剂。其中，酰胺类除草剂药效低，对环境污染大；磺酰脲类除草剂残留量大，药害较重。随着我国农业的迅速发展和对环境的重视，这两类除草剂急需更新换代。ACCase抑制剂类除草剂是较理想的换代产品，而烯草酮是其中的一种代表性品种，有很好的开发应用前景(郭林华，王鹏.除草剂烯草酮的合成研究进展[J].现代农药，2006 5(1)：5-8)。为此，研究开发具有ACCase抑制剂类除草剂抗性(耐受性)的禾本科作物(水稻)，这样可以在种植期间使用除草剂，杀死杂草，但不影响植物本身的生长，由此拓宽了除草剂的使用范围。
本发明正是经过长期而艰苦的研究实践，凭借运气偶然地利用烯草酮除草剂在水稻突变体库中发现了两个具有ACCase抑制剂类除草剂抗性的突变体，所述抗性突变体可以耐受ACCase抑制剂类除草剂，尤其是烯草酮。进一步的，在原有的两个突变体基础上进行再次诱变，利用精喹禾灵除草剂进行筛选，偶然的获得了多个抗性位点聚合的具有ACCase抑制剂类除草剂抗性的突变体。本发明还开发了具有ACCase抑制剂类除草剂抗性的蛋白及其编码基因在转基因或者非转基因植物育种中的应用，所述除草剂抗性基因和蛋白可用于培育具有除草剂抗性的植物，尤其是禾本科农作物，如水稻、玉米、小麦、大麦、高粱、黍、谷子等。
发明内容
本发明提供了一种对乙酰辅酶A羧化酶(ACCase)抑制剂具有耐受性的突变体植株，所述突变体植株的基因组DNA发生了突变。与野生型黄华占对照相比，突变体植株可以耐受ACCase抑制剂类除草剂，所述ACCase抑制剂类除草剂包含APP(芳氧苯氧丙酸)类、CHD(环已烯酮)类以及DEN(苯基吡唑啉)类，其中APP类包括精喹禾灵(quizalofop-e-ethyl)，禾草灵(diclofop-methyl)，炔草酯(clodinafop-propargyl)等主要品种；CHD类包括肟草酮(tralkoxydim)，噻草酮(cycloxdim)，稀禾啶(sethoxydim)，烯草酮(clethodim)，禾草灭(alloxydim)等主要品种；DEN类主要代表药剂有唑啉草酯(pinoxaden)。
在水稻植株中，野生型的乙酰辅酶A羧化酶(ACCase)基因位于水稻第五号染色体上(Genebank Accession NO：AP014961.1，基因区域：13063871-13078401)，其基因组DNA序列如SEQ ID NO：1所示，其编码区的核苷酸序列如SEQ ID NO：2所示，所编码的氨基酸序列如SEQ ID NO：3所示。
本发明所提供的抗除草剂水稻突变体植株中，第一种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5372位点发生突变，核苷酸由A变为G，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：4所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：5所示。第二种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5374位点发生突变，核苷酸由A变为T，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的编码区核苷酸序列如SEQ IDNO：6所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：7所示。第三种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第6319位点发生突变，核苷酸由G变为A，导致相应编码蛋白的第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ IDNO：8所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：9所示。第四种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5374和第6319位点发生突变，核苷酸分别由A变为T，和由G变为A，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：10所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：11所示。第五种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5372和第5374位点发生突变，核苷酸分别由A变为G，和由A变为T，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：12所示，其编码的ACCase蛋白的氨基酸序列如SEQID NO：13所示。第六种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5372和第6319位点发生突变，核苷酸分别由A变为G，和由G变为A，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：14所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：15所示。
本发明还提供了一种使水稻组织细胞、植株、品种、品系或杂交种获得除草剂抗性的方法，并通过此方法获得具有除草剂抗性的水稻组织细胞、植株、品种、品系或杂交种。所述方法包括以下几种实施方式：
获得除草剂抗性的实施方式之一，通过突变水稻内源的ACCase基因，使该植物体产生对ACCase抑制剂类除草剂抗性的过程。所述“突变”包括但不限于以下方法，如用物理或化学的方法导致的基因突变，化学方法包括用EMS等诱变剂处理所导致的诱变，所述突变还可以是点突变，也可以是DNA缺失或插入突变，还可以是通过RNAi、基因定点突变等基因沉默手段产生。所述基因定点突变的方法包括但不限于ZFN定点突变方法、TALEN定点突变方法、和/或CRISPR-Cas9等基因组定点突变方法。本领域技术人员应该知晓，在本发明公开了水稻内源的ACCase基因序列，及其突变后具有除草剂抗性的位点后，可以通过ZFN、TALEN和/或CRISPR-Cas9等基因修饰的方法得到本发明所提供的突变体核苷酸序列和蛋白序列及突变体植株。
获得除草剂抗性植株的方法，还包括通过转基因的方法获得，具体是指将本发明获得的具有除草剂抗性的ACCase突变体核苷酸序列，通过转基因的方法转入目标植株，使目标植株获得对ACCase抑制剂类除草剂的抗性。还可通过杂交、转育、回交等方法，将本发明中获得的ACCase突变位点转移到目标植株中，使目标植株获得对ACCase抑制剂类除草剂的抗性。更具体的，可将该突变体作为亲本材料，与其它优良水稻品种杂交并进一步回交，把抗除草剂性状进一步转育到其它目标品种中。
本发明上述的转基因方法是使用植物生物技术领域技术人员已知的转化方法制备。所述转化方法包括直接或间接的转化方法。直接转化方法包括聚乙二醇诱导的DNA摄入、脂质体介导的转化、使用基因枪导入、电穿孔、以及显微注射，等。在本发明的具体实施方式中，本发明使用了基于土壤杆菌的转化技术(可参见Horsch RB等(1985)Science 225：1229；White FF，Vectors for Gene Transfer in Higher Plants，Transgenic Plants，第1卷，Engineering and Utilization，Academic Press，1993，pp.15-38；Jenes B等.Techniques for Gene Transfer，Transgenic Plants，第1卷，Engineering andUtilization，Academic Press，1993，pp.128-143，等)。土壤杆菌菌株(例如根瘤土壤杆菌或毛根土壤杆菌)包含质粒(Ti或Ri质粒)和T-DNA元件，所述质粒和元件在用土壤杆菌转染后被转移至植物，而T-DNA被整合进植物细胞的基因组中。T-DNA可位于Ri-质粒或Ti-质粒上，或独立地包含在所谓的双元载体中。土壤杆菌介导的转化方法描述于例如中。土壤杆菌介导的转化最适合双子叶植物，但是也适合单子叶植物。所述转化可导致瞬时或稳定的转化和表达。本发明所提供的ACCase突变体核苷酸序列可被转入到任一种植物和植物细胞中，优选的尤其适用于转入到农作物植物细胞，如玉米、小麦、大麦、高粱、黍、水稻、谷子等禾本科植物。
本发明中所提到的抑制乙酰辅酶A羧化酶(ACCase)活性的除草剂，又称为ACCase抑制剂类除草剂，共分为3种类型，分别是APP(芳氧苯氧丙酸)类、CHD(环已烯酮)类以及DEN(苯基吡唑啉)类。其中，已经商品化的ACCase抑制剂类除草剂产品有氰氟草酯、高氟吡禾灵、精氟吡禾草灵、喹禾糠酯、禾草灭、烯禾啶、噻草酮、烯草酮、苯草酮等。本发明所提供的ACCase突变体蛋白和突变体植株，均对上述除草剂具有耐受能力。
本发明还提供了一种ACCase突变体蛋白，具体而言，本发明提供了使植物具有ACCase抑制剂类除草剂抗性的ACCase突变蛋白质，该蛋白质能使禾本科作物(如水稻、玉米、小麦、高粱、大麦、黍、谷子等)具有ACCase抑制剂类除草剂抗性。在本文中，ACCase就是乙酰辅酶A羧化酶(AcetyCoA carboxylase，[EC6.4.1.2])的简称，ACCase(乙酰辅酶A羧化酶)是调节脂肪酸生物合成的一种关键酶，它催化真核生物与原核生物脂肪酸生物合成的第一阶段。在植物体内有2种类型的ACCase，其中第一种固定于叶绿体内，是主要脂肪酸生物合成中的关键酶，第二种则存在于胞液中，涉及长侧链脂肪酸生物生物合成。禾本科及具有相对敏感型ACCase的植物能够被ACCase抑制剂类除草剂所杀死。
本发明通过实验发现，水稻ACCase基因的一个突变能够导致含有该突变的植株对ACCase抑制剂类除草剂的抗性。第一种突变为乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5372位点发生突变，核苷酸由A变为G，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：4所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：5所示。第二种突变为乙酰辅酶A羧化酶(ACCase)基因编码区的第5374位点由A突变为T，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的编码核苷酸序列如SEQ ID NO：6所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：7所示；第三种突变是，乙酰辅酶A羧化酶(ACCase)基因的第6319位点发生突变，核苷酸由G变为A，导致相应编码蛋白的第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：8所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：9所示。第四种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5374和第6319位点发生突变，核苷酸分别由A变为T，和由G变为A，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：10所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：11所示。第五种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5372和第5374位点发生突变，核苷酸分别由A变为G，和由A变为T，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：12所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：13所示。第六种突变体是，乙酰辅酶A羧化酶(ACCase)基因编码区序列的第5372和第6319位点发生突变，核苷酸分别由A变为G，和由G变为A，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：14所示，其编码的ACCase蛋白的氨基酸序列如SEQID NO：15所示。
本发明还提供了一种适于水稻直播的方法，所述方法包括：使用于直播的水稻品种获得本发明所提及的ACCase抑制剂类除草剂抗性，所述抗性可以通过转基因的方法获得，也可以通过上述突变或诱变的方法获得，或是通过杂交、回交、转育的方法获得，具有该除草剂抗性的植株体内的ACCase基因编码区的第5372位点发生突变，核苷酸由A变为G，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：4所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：5所示；或者是ACCase基因编码区的第5374位点由A突变为T，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的编码核苷酸序列如SEQ ID NO：6所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：7所示；或者是ACCase基因编码区的第6319位点发生突变，核苷酸由G变为A，导致相应编码蛋白的第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：8所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：9所示；或者ACCase基因编码区序列的第5374和第6319位点发生突变，核苷酸分别由A变为T，和由G变为A，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：10所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：11所示；或者ACCase基因编码区序列的第5372和第5374位点发生突变，核苷酸分别由A变为G，和由A变为T，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：12所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：13所示；或者ACCase基因编码区序列的第5372和第6319位点发生突变，核苷酸分别由A变为G，和由G变为A，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：14所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：15所示。获得该除草剂抗性植株后，将其种子播到田中，在幼苗期，用ACCase抑制剂类除草剂或含有ACCase抑制剂类除草剂的混剂进行喷施，使田中的杂草或杂草稻死亡，而具有ACCase抑制剂类除草剂抗性的目标植株健康成长。本发明所提供的具有除草剂抗性的ACCase突变体蛋白、核苷酸序列和抗性植株，可以用于水稻直播过程中的杂草管理，解决了直播过程中的杂草管理这个难题，使水稻获得了ACCase抑制剂类除草剂的抗性这一新的农业性状，为水稻田间杂草(尤其是禾本科杂草)管理提供了新方法，减少了田间除草成本，便于田间管理，推进了水稻直播技术更快更好的发展。
本发明还提供了一种防除水稻落田谷的方法，所述方法包括：使种植的水稻品种获得本发明所提供的ACCase抑制剂类除草剂抗性，所述抗性可以通过转基因的方法获得，也可以通过上述突变或诱变的方法获得，或是通过杂交、回交、转育的方法获得，具有该除草剂抗性的植株体内的ACCase基因编码区的第5372位点发生突变，核苷酸由A变为G，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：4所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：5所示；或者是ACCase基因编码区的第5374位点由A突变为T，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的编码核苷酸序列如SEQ ID NO：6所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：7所示；或者是ACCase基因编码区的第6319位点发生突变，核苷酸由G变为A，导致相应编码蛋白的第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：8所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：9所示；或者ACCase基因编码区序列的第5374和第6319位点发生突变，核苷酸分别由A变为T，和由G变为A，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：10所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：11所示；或者ACCase基因编码区序列的第5372和第5374位点发生突变，核苷酸分别由A变为G，和由A变为T，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：12所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：13所示；或者ACCase基因编码区序列的第5372和第6319位点发生突变，核苷酸分别由A变为G，和由G变为A，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：14所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：15所示。获得该除草剂抗性植株后，将其种子播到田中，在幼苗期，用ACCase抑制剂类除草剂或含有ACCase抑制剂类除草剂的混剂进行喷施，使上一季水稻收割后掉落在田间的不具有ACCase抑制剂类除草剂抗性的种子生长出的幼苗死亡，而具有ACCase抑制剂类除草剂抗性的目标植株健康成长。
本发明还提供了一种防除田间抗性杂草的方法。在生产应用中，同一种水稻除草剂连续施用，由于筛选压力的积累，会很快在稻田中产生抗性杂草和抗性杂草稻。将具有ACCase抑制剂类除草剂抗性的水稻与其他具备不同除草剂抗性的水稻结合使用，每种植1-3个季度轮换一次水稻抗性，可以有效防治抗性杂草的产生及危害。所述方法包括：使种植的水稻品种获得本发明所提的ACCase抑制剂类除草剂抗性，所述抗性可以通过转基因的方法获得，也可以通过上述突变或诱变的方法获得，或是通过杂交、回交、转育的方法获得。具有该除草剂抗性的植株体内的ACCase基因编码区的第5372位点发生突变，核苷酸由A变为G，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：4所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：5所示；或者是ACCase基因编码区的第5374位点由A突变为T，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的编码核苷酸序列如SEQ ID NO：6所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：7所示；或者是ACCase基因编码区的第6319位点发生突变，核苷酸由G变为A，导致相应编码蛋白的第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：8所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：9所示；或者ACCase基因编码区序列的第5374和第6319位点发生突变，核苷酸分别由A变为T，和由G变为A，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：10所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：11所示；或者ACCase基因编码区序列的第5372和第5374位点发生突变，核苷酸分别由A变为G，和由A变为T，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：12所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：13所示；或者ACCase基因编码区序列的第5372和第6319位点发生突变，核苷酸分别由A变为G，和由G变为A，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：14所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：15所示。获得该除草剂抗性植株后，将其种子播到田中，在幼苗期，用ACCase抑制剂类除草剂或含有ACCase抑制剂类除草剂的混剂进行喷施，使由于田间产生的具有其他除草剂抗性的杂草幼苗死亡，而具有ACCase抑制剂类除草剂抗性的目标植株健康成长。采用该方法连续种植1-3个生长季后，更换种植具有其他除草剂抗性的水稻以及相应的除草剂，将田间可能积累的具备ACCase抑制剂类除草剂的抗性杂草灭除。同样，连续种植具有其他除草剂抗性的水稻并应用相应的除草剂后，更换种植具有ACCase抑制剂类除草剂的水稻，可将田间可能积累的具有其他类型除草剂抗性的抗性杂草灭除。
本发明还提供了一种提高杂交种子纯度的杂交育种方法，所述方法包括将本发明提供的具有ACCase抑制剂类除草剂抗性性状的植株作为父本，或是通过传统杂交回交手段转育到杂交育种父本植物中，与不具有ACCase抑制类除草剂抗性的的母本杂交，产生的种子用ACCase抑制剂类除草剂处理，清除母本自交产生的种子，从而提高杂交种子纯度。本发明所提供的ACCase突变体蛋白、核酸和突变体植株，为培育水稻杂交新品种、提高杂交种子纯度提供了新方法和新材料。
本发明所提供的对ACCase抑制剂类除草剂不敏感的基因序列，本领域技术人员可以通过常规的分子生物学技术，就可以制备出取代、添加或缺失了氨基酸残基的ACCase蛋白质，这些方法记载于《分子克隆实验指南》(北京：科学出版社，2002年)等文献中。通过转基因技术，将编码这些具有ACCase抑制剂类除草剂抗性蛋白质的基因导入植物中，优选导入禾本科植株中，更具体的导入水稻植株中，从而赋予植物对乙酰辅酶A羧化酶(ACCase)抑制剂类除草剂的抗性。
本发明取得的有益效果在于获得可替代现有技术的使植物具有除草剂抗性的蛋白质、核酸、表达盒、载体、细胞、植物，获得具有除草剂抗性的植物的应用和方法、以及鉴定本发明的植物的方法，并且可以通过转基因或非转基因方法获得具有除草剂抗性的植物品种，尤其是禾本科植物品种，优选为水稻品种。
本发明还提供了一种表达盒、表达载体或工程菌株，所述表达盒、表达载体或工程菌株含有本发明所提的ACCase突变体核苷酸序列，更具体的含有本发明所提供的突变位点A5372G和/或A5374T和/或G6319A，或是含有编码本发明所提供的核苷酸序列SEQ ID NO：4、SEQ ID NO：6、SEQ ID NO：8、SEQ ID NO：10、SEQ ID NO：12或SEQ ID NO：14，或是包含本发明所提供的氨基酸序列SEQ ID NO：5、SEQ ID NO：7、SEQ ID NO：9、SEQ ID NO：11、SEQ IDNO：13或SEQ ID NO：15，所述表达盒、表达载体或工程菌株可以用于使植株或植株细胞获得对ACCase抑制剂类除草剂的抗性。
本发明还提供了一种使植物叠加不同除草剂抗性的方法，所述方法包括，在通过上述方法获得本发明所提供的ACCase除草剂抗性突变体后，再通过转基因、或基因组编辑、或物理化学诱变的方法，或是通过与具有其他除草剂抗性的植株进行杂交、回交，从而目标植株获得两种或两种以上除草剂抗性的方法，所述除草剂抗性包括ALS基因突变产生的对咪唑啉酮类除草剂的抗性,或是通过转入草甘膦、草铵膦、莠去津、溴苯腈、2，4-D、咪唑啉酮和磺酰脲类等除草剂的抗性基因而获得多重抗性，所述除草剂抗性基因包括但不限于aroA基因、EPSP基因、Bar基因、tfdA基因等。
此外，本发明所提供的ACCase突变体还可以叠加其他性状，所述性状包括但不限于抗虫性状，例如BtCry和其他对于鞘翅类、鳞翅类、线虫类或其他害虫具有杀虫活性的蛋白质；提高营养或药用价值的性状，例如改变油含量或油成分的性状、高蛋白质或高氨基酸浓度性状，和其他本领域中已知的性状类型；增加生物产量的性状，例如光合产量、谷粒产量、淀粉含量、油含量或蛋白质含量等；对干旱、盐、高温等非生物逆境产生耐受性的性状；或是对害虫、病毒性病原体等生物逆境产生耐受性的性状。
更具体的，本发明提供了获得具有除草剂抗性的植物的方法，其包括如下步骤：
(1)使植物包含本发明所提供ACCase蛋白突变位点N1791S突变，或I1792L突变，或G2107S突变，或I1792L和G2107S双突变，或N1791S和I1792L双突变，或N1791S和G2107S双突变；和/或
(2)测定所述植物是否包含本发明所提供的核苷酸序列SEQ ID NO：4、SEQ ID NO：6、SEQ ID NO：8、SEQ ID NO：10、SEQ ID NO：12或SEQ ID NO：14；和/或
(3)使植物表达本发明的蛋白质氨基酸序列SEQ ID NO：5、SEQ ID NO：7、SEQ IDNO：9、SEQ ID NO：11、SEQ ID NO：13或SEQ ID NO：15。
可以采用前述或者本领域技术人员能获知的转基因和非转基因方法，使植物包含本发明所提供的核苷酸序列SEQ ID NO：4、SEQ ID NO：6、SEQ ID NO：8、SEQ ID NO：10、SEQID NO：12或SEQ ID NO：14，或者使植物表达本发明所提供的蛋白质氨基酸序列SEQ ID NO：5、SEQ ID NO：7、SEQ ID NO：9、SEQ ID NO：11、SEQ ID NO：13或SEQ ID NO：15。所述获得除草剂抗性的方法可以采用的步骤包括转基因、杂交、回交、自交或无性繁殖步骤。这些步骤本身对于转基因或非转基因育种领域的技术人员来说，都是可以获知并实施的。
本发明还提供了鉴定本发明所提供的抗除草剂植物的方法，其包括如下步骤：
(1)测定所述植物是否包含本发明所提供ACCase蛋白突变位点N1791S突变，或I1792L突变，或G2107S突变，或I1792L和G2107S双突变，或N1791S和I1792L双突变，或N1791S和G2107S双突变；和/或
(2)测定所述植物是否包含本发明所提供的核苷酸序列SEQ ID NO：4、SEQ ID NO：6、SEQ ID NO：8、SEQ ID NO：10、SEQ ID NO：12或SEQ ID NO：14；和/或
(3)测定所述植物是否表达本发明所提供的氨基酸序列SEQ ID NO：5、SEQ ID NO：7、SEQ ID NO：9、SEQ ID NO：11、SEQ ID NO：13或SEQ ID NO：15。
通过该方法，可以判断植物是否属于本发明所提供的植物或是通过本发明所提供的方法获得了该除草剂抗性植株。测定的步骤可以通过常规的核酸检测和/或蛋白质检测方法来进行，只需要能够检测出蛋白质或其编码核酸带有N1791S突变，或I1792L突变，或G2107S突变，或I1792L和G2107S双突变，或N1791S和I1792L双突变，或N1791S和G2107S双突变，或其相应核苷酸突变的方法均可。示例性的方法包括蛋白质测序、核酸测序、聚合酶链式反应(PCR)检测、探针杂交检测等。
本发明取得的有益效果在于获得可替代现有技术的使植物具有除草剂抗性的蛋白质、核酸、表达盒、载体、细胞、植物、获得具有除草剂抗性的植物的应用和方法、以及鉴定本发明的植物的方法，并且可以通过转基因或非转基因方法获得具有除草剂抗性的植物品种，尤其是水稻品种。
本发明引用了公开文献，这些文献是为了更清楚地描述本发明，它们的全文内容均纳入本文进行参考，就好像它们的全文已经在本文中重复叙述过一样。为了便于理解，以下将通过具体的附图和实施例对本发明进行详细地描述。需要特别指出的是，这些描述仅仅是示例性的描述，并不构成对本发明范围的限制。依据本说明书的论述，本发明的许多变化、改变对所属领域技术人员来说都是显而易见的了。
附图说明
图1是筛选获得的抗烯草酮除草剂突变体植株。
图2是突变株系KX2-2与黄华占(WT)对照的苗期烯草酮喷施实验，其中0、35、70、105、140、175、210表示施用剂量，单位为g a.i./hm2。
图3是突变株系KX2-28与黄华占(WT)对照的苗期烯草酮喷施实验，其中0、35、105、175、210表示施用剂量，单位为g a.i./hm2。
图4是突变株系KX3-89与黄华占(WT)对照的苗期烯草酮喷施实验，其中0、35、105、175、210表示施用剂量，单位为g a.i./hm2。
图5是二次诱变后筛选获得的突变体植株。
图6是六种突变株系对于不同ACCase抑制剂类除草剂抗性实验的结果统计，其中CK表示对照(control check)，黄华占为野生型对照株系。
具体实施方式
实施例1、诱变获得抗烯草酮除草剂的突变体
播种水稻黄华占种子，播种前将黄华占种子在25℃培养箱中用水浸泡22小时后，将种子捞出，控干水分。然后，用0.7％(w/w)甲磺酸乙酯(EMS)浸泡水稻种子，浸泡12小时，期间每1小时摇动一次种子。12小时后弃去EMS溶液，换自来水浸泡种子，弃自来水，浸泡共重复5次，每次浸泡5分钟，然后用自来水冲洗种子2小时，冲洗过程中翻动种子，从而将EMS洗净。取少量种子放在湿纸表面，放入28℃培养箱中，测定发芽率为60～70％，剩余的种子播种于大田。待植株成熟后分地块收割，每个地块面积30平方米，混收，晾干至含水量8％。将收割的种子浸泡处理10h后播种，播种密度为1.86万棵/m2，待秧苗成长至4叶期，用除草剂烯草酮(clethodim)按照60g a.i./hm2的剂量喷施处理，15天后调查秧苗生长情况，得到9株存活秧苗，其中一颗存活苗如图1所示，确认它们均为黄华占遗传背景的抗烯草酮除草剂突变株。保留并繁殖这些绿色秧苗，这些具有烯草酮除草剂抗性的植株均能够正常生长发育，其余非抗性水稻均死亡。
实施例2、抗烯草酮除草剂突变体的基因组序列分析
取上述水稻突变植株的叶片，连同野生型黄华占的水稻植株叶片，分别提取它们的基因组DNA，委托深圳华大基因研究院进行测序，序列对比发现：相对于野生型黄华占，其中一个突变植株的乙酰辅酶A羧化酶(ACCase)在第5372位点发生突变，由A变为G，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，所述突变后的ACCase基因的核苷酸序列如SEQ ID NO：4所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：5所示，进行回交纯化，自交扩繁后获得稳定株系KX2-28；另外三个突变植株的乙酰辅酶A羧化酶(ACCase)在同一点上发生了变异，具体突变是ACCase基因的第5374位点发生突变，由A变为T，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，所述突变后的ACCase基因的核苷酸序列如SEQ IDNO：6所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：7所示，选取其中一个单株进行回交纯化，自交扩繁后获得稳定株系KX2-2；在其余五个突变植株中，其乙酰辅酶A羧化酶(ACCase)的基因均在第6319位点发生突变，由G变为A，导致相应编码蛋白的第2107位由甘氨酸变为丝氨酸，所述突变后的ACCase基因的核苷酸序列如SEQ ID NO：8所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：9，选取其中一个单株进行回交纯化，自交扩繁后获得稳定株系KX3-89。
实施例3、突变体KX2-2、KX2-28和KX3-89的苗期喷施实验
将已获得的水稻抗ACCase抑制剂类除草剂的突变株系KX2-2、KX2-28、KX3-89以及野生型黄华占种子(WT)分别播种。野生型水稻种子(WT)与突变体KX2-2在4叶期分别喷施0、35、70、105、140、175、210g a.i./hm2施用剂量的烯草酮验证抗性，实验结果如图2所示，在35g a.i./hm2剂量的烯草酮处理下，野生型黄华占大部分死亡，生长发育受到极大影响，而KX2-2不受烯草酮的影响，表现出很强的烯草酮抗性；当烯草酮施用剂量达到105g a.i./hm2时，野生型黄华占全部死亡，而抗性突变株系KX2-2仍有约40％的存活。
野生型水稻种子(WT)与突变株系KX2-28在4叶期分别喷施0、35、105、175、210ga.i./hm2施用剂量的烯草酮验证抗性，结果如图3所示，在35g a.i./hm2剂量的烯草酮处理下，野生型黄华占的生长发育受到了极大的抑制，而突变体的生长发育正常，当烯草酮施用剂量达到105g a.i./hm2时，野生型黄华占基本死亡，突变株系KX2-28仍然有约50％的存活。
野生型水稻种子(WT)与突变株系KX3-89在4叶期分别喷施0、35、105、175、210ga.i./hm2施用剂量的烯草酮验证抗性，结果如图4所示，在35g a.i./hm2剂量的烯草酮处理下，野生型黄华占的生长发育受到了极大的抑制，而突变体的生长发育正常，当烯草酮施用剂量达到105g a.i./hm2时，野生型黄华占基本死亡，突变株系KX3-89仍然有约40％的存活。本实验说明本发明所获得的抗性突变体KX2-2、KX2-28和KX3-89与野生型相比，具有对ACCase抑制剂类除草剂烯草酮的抗性。
实施例4、诱变获得具有双突变位点的突变体
播种KX2-2、KX2-28和KX3-89，播种前将这三个株系的种子在25℃培养箱中用水浸泡22小时后，将种子捞出，控干水分。然后，用0.7％(w/w)甲磺酸乙酯(EMS)浸泡水稻种子，浸泡12小时，期间每1小时摇动一次种子。12小时后弃去EMS溶液，换自来水浸泡种子，弃自来水，浸泡共重复5次，每次浸泡5分钟，然后用自来水冲洗种子2小时，冲洗过程中翻动种子，从而将EMS洗净。取少量种子放在湿纸表面，放入28℃培养箱中，测定发芽率为60～70％，剩余的种子播种于大田。待植株成熟后分地块收割，每个地块面积30平方米，混收，晾干至含水量8％。将收割的种子浸泡处理10h后播种，播种密度为1.86万棵/m2，待秧苗成长至4叶期，用除草剂精喹禾灵(quizalofop-e-ethyl)(购自湖南农大海特农化有限公司)按照300g a.i./hm2的剂量进行喷施处理，15天后调查秧苗生长情况，得到33株存活秧苗，其中一颗存活苗如图5所示，确认它们均为黄华占遗传背景的抗ACCase抑制剂类除草剂的突变株。
取上述水稻突变植株的叶片，连同野生型黄华占的水稻植株叶片，分别提取它们的基因组DNA，委托深圳华大基因研究院进行测序，序列对比发现：相对于野生型黄华占，10个突变株的ACCase基因编码区序列与KX2-2相同；7个突变株的ACCase基因编码区序列与KX2-28相同，8个突变株的ACCase基因编码区序列与KX3-89相同；4个突变植株的ACCase基因编码区序列的第5374和第6319位点发生突变，核苷酸分别由A变为T，和由G变为A，导致相应编码蛋白的第1792位由异亮氨酸变为亮氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：10所示，其编码的ACCase蛋白的氨基酸序列如SEQID NO：11所示，取其中一个单株回交纯化，自交扩繁后获得稳定株系KX4-12；2个突变植株的ACCase基因编码区序列的第5372和第5374位点发生突变，核苷酸分别由A变为G，和由A变为T，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第1792位由异亮氨酸变为亮氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：12所示，其编码的ACCase蛋白的氨基酸序列如SEQ ID NO：13所示，取其中一个单株回交纯化，自交扩繁后获得稳定株系KX4-14；2个突变植株的第5372和第6319位点发生突变，核苷酸分别由A变为G，和由G变为A，导致相应编码蛋白的第1791位由天冬酰胺变为丝氨酸，第2107位由甘氨酸变为丝氨酸，突变后的ACCase基因的核苷酸序列如SEQ ID NO：14所示，其编码的ACCase蛋白的氨基酸序列如SEQID NO：15所示，取其中一个单株回交纯化，获得稳定株系KX4-20。
实施例5、ACCase抗除草剂突变体的苗期喷施实验
将已获得的水稻抗ACCase类除草剂的突变株系KX2-2、KX2-28、KX3-89、KX4-12、KX4-14、KX4-20与黄华占种子分别播种，在水稻生长至4叶期时，分别喷施烯草酮(喷施剂量105g a.i./hm2)、精喹禾灵(喷施剂量300g a.i./hm2)、禾草灵(喷施剂量1080g a.i./hm2)和唑啉草酯(喷施剂量75g a.i./hm2)，检验突变株系对于ACCase类除草剂的抗性水平。实验结果如图6所示，施药20天后，在本实施例所用的除草剂浓度下，普通水稻品种黄华占作为对照大部分已经死亡，而KX2-2、KX2-28、KX3-89、KX4-12、KX4-14和KX4-20六个突变株系则表现出对上述除草剂很强的抗性，在本实施例所用的除草剂浓度下，突变体株系的苗基本存活。本实施例说明本发明所获得的株系KX2-2、KX2-28、KX3-89、KX4-12、KX4-14和KX4-20，具有对ACCase抑制剂类除草剂的抗性。
实施例6、利用抗ACCase抑制剂类突变体防除落粒谷
在水稻大田生产过程中，经常会出现上一茬水稻在机械收割或自然成熟过程中，水稻籽粒掉落田间，形成落田谷。在下一茬水稻种植生产时，落田谷自然生长出来，在田间形成杂株，影响水稻生产管理以及稻谷的品质、产量。利用抗咪唑啉酮类除草剂水稻和配套除草剂共同应用，可以有效杀灭田间的落田谷，但这类水稻同样也会存在落田谷问题，而且下一季的落田谷，同样具备此类抗性。如果希望解决落田谷问题，就需要两种不同类型的抗除草剂水稻交替使用。
在大田种植抗咪唑啉酮类除草剂水稻，该水稻如我司已授权专利(申请号CN201210037789.9)中所述具备对于咪唑啉酮类除草剂的抗性，其ALS基因在1642和1643位点的碱基由TG突变成AT，并导致对应的蛋白质位点由色氨酸突变为甲硫氨酸。正常管理并机械收获后，下茬作物种植抗ACCase抑制剂类除草剂突变体KX2-2，在水稻3叶期茎叶喷施烯禾啶，亩施用有效成分为20g。15后对田间水稻苗取三个小区，每个小区面积1平方米，喷施咪唑乙烟酸，亩施用有效成分200g，结果如表1所示。
表1防除落粒谷试验结果统计
根据表1可知，在交替使用抗咪唑啉酮类除草剂和抗ACCase抑制剂类除草剂水稻后，上一茬水稻的落田谷在喷施完当季的除草剂后，被全部杀死，不会在田间形成杂株。
在生产应用中，同一种水稻除草剂连续施用，由于筛选压力的积累，会很快在稻田中产生抗性杂草和抗性杂草稻。基于与防治落粒谷同样的原理，交替使用不同除草剂抗性的水稻，以抗ACCase抑制剂类除草剂水稻和抗咪唑啉酮类除草剂水稻为例，每使用同一种除草剂1-3个生长季后，替换使用另一种除草剂，可以有效防止田间抗性杂草的出现。
实施例7、抗性突变体序列的转基因功能验证
本发明通过基因克隆的方式，获得了实施例2和实施例3中的突变体ACCase基因编码序列，分别如SEQ ID NO：4、SEQ ID NO：6、SEQ ID NO：8、SEQ ID NO：10、SEQ ID NO：12或SEQ ID NO：14所示，将该序列用组成型启动子ubi进行驱动表达，转入常规水稻品种武运粳7号中，获得转基因阳性苗后，进行苗期的喷施实验，按照280g a.i./hm2的用量喷施烯禾啶，结果发现：野生型武运粳对照全部死亡，而转基因阳性苗的生长不受除草剂喷施的影响，表明突变体序列通过转基因方式转入植株中，也可以赋予所转入植株对ACCase抑制剂类除草剂的抗性。
序列表
<110> 未名兴旺系统作物设计前沿实验室（北京）有限公司
深圳兴旺生物种业有限公司
<120> 除草剂抗性突变体及其应用
<150> 201710089089.7
<151> 2017-02-20
<160> 15
<170> SIPOSequenceListing 1.0
<210> 1
<211> 14531
<212> DNA
<213> Oryza sativa
<400> 1
caggcctaaa ttccattcag agtttcctct gccactgccg cagtcaaaca aaccgtgcaa 60
acagggacgg gcagtcacac accaagagag cgaaacgagc gcgcctgccg gtcgcgaaaa 120
ccctgggaac tcgtcacatc tggtgcgccg ggagcatccc ggccgtccac ggccccgccc 180
atcctacagc tggacgcgat ccctgccgtc catccggcgc acctccctcg ctgtcacgtg 240
cacggaggag gagcgggggg gggggggggg ggggggggga ggaggggggc ggagtcccca 300
atgccgatgc ggccgtggga atttattatt ttaggccgca cagcccccat ctctccccca 360
cccctcggga tatccgcctt ctcccgcccg ctccgcctcc gcctccgcct ccgcctcgcc 420
gccgttgtcg acgccgcaag gatccaaacg ccgccccgcg tcgccttctc cgccttcttc 480
ttcttcttct tcttcttctc cgagggtctc tctcgggaag gtacagctgc ccgcctccct 540
gctcttattt atcgcgtgcc gttcattccg tgccttctcc agctccagat ccgcgcgccg 600
cacgccgatc cgcgcccggc cggtgagtcc gccgcgggtt tccgccattg ctgccgtttc 660
tgcgctgcta gggggaggtt tagatgtggg tggtgggagc cgcgtgctgt tgtctgggat 720
gggagagaga gggtttaagg gtgggtgccg gatgcggatg ctcccgcccg ggttttggtc 780
ctgcttgtgg actggcgagg ggctaggggt tgagttgtag ggattcggga tggaaatgga 840
ctggggagct cggatgggga ggtaatgcca cgccggtgaa atgcagctcg ctgttgttgg 900
ttctggttaa tgcctgggtg ttgctggcta tgttactatg ttgcatgcct acgcgagata 960
ctgccatgtg ccattttgcg tgattgtgat attggtttga ggttttagct gcgctcatca 1020
tttcctcgcg ttggctcagg gtttaagctc ctctttgtta agtgggacgg tatgcatatg 1080
cattgtgttc ttttaggttt aaattctggt tacggtgcca cacaacaagt cgtagatgct 1140
cattcttgtt cttttcatgg ggcttatgga attgatggca catggcacac ctttctcctt 1200
tttccgtcat gcatgttagt gccatattgt ctacgcaaaa aagaatattc tgcatatttg 1260
tttactcact atttttttcc aagagaattg ggttttgcca tgcttcaaaa ggaacatttc 1320
aagctgaaaa agttctcccg atatggaatt agctttagag ttcaggggaa aaaatatgtt 1380
gttgctttgt gcttgtcaag taaccacagt tgcacaggtt ttgactgacc tcatgctact 1440
tgacattatt caccatgata aagttttacc ttttgttttt ttcctttcac taggaacaga 1500
acgaattgtt tgcataacca atttgtagat ttgacatggg gaaatagcat aactgaatat 1560
atctcaacct attactaggc aaaataagaa atgaacctaa gccttaacag gtttgcatca 1620
aattatcctg tgcaaaatga acaaaggtgg cacacaatgt acaagcttgc atttcatttt 1680
tggatcctca aacgtcttaa agacttttgc ataaagtatt tgatgagtaa cgagctggaa 1740
ctaatcattc ttatatattt tcatctgtca gatttcacac atctggggat ttatcttctc 1800
tttgtatggc actacacatt tgagaaaccg tgcaattcta ctgtttggtc agcaggacaa 1860
caatgacatc cacacatgtg gcgacattgg gagttggtgc ccaggcacct cctcgtcacc 1920
agaaaaagtc agctggcact gcatttgtat catctgggtc atcaagaccc tcataccgaa 1980
agaatggtca gcgtactcgg tcacttaggg aagaaagcaa tggaggagtg tctgattcca 2040
aaaagcttaa ccactctatt cgccaaggtg accactagct actttacata tgctataatt 2100
tgtgccaaac ataaacatgc aatggctgct attatttaaa cgttaatgtt gaaatagctg 2160
ctataggata cagcaaaaat atataattga ctgggcaaga tgcaacaatt gtttttcact 2220
aaagttagtt atcttttgct gtaaaagaca actgtttttt acataaaatg gtattaataa 2280
ccttgtaata ttcaatgcaa catgttctca agtaaaaaaa aacattgcct ggttgtataa 2340
gcaaatgtgt cgttgtagac atcttattaa acctttttgt gatatctatt accgtaggga 2400
acaggggagc tgtttaaatc tgttatcata gagtaatatg agaaaagtgg attgtgcgac 2460
tttggcatgt atacctgctc aatttcaaat atatgtctat gtgcaggtct tgctggcatc 2520
attgacctcc caaatgacgc agcttcagaa gttgatattt cacagtaagg actttatatt 2580
ttataataat tattatataa ttttctgaca tgttttgaga acctcaaaac atgtgattgc 2640
accttccttt tttatgtctg gttcagaaac tgataagttt tgacagtgtt taggatggat 2700
ctttgatgcg cacagtgctt tctaatgttt tcatttttga aagtaatgtt ttaggaagaa 2760
atatctgatt aaatttatac tttatcttta caaaagtcaa atgcgttctg tatcaattgc 2820
ggtttgtaat atggcaagaa catgctttca gaatttgttc atacaatgct ttctttctat 2880
tattatgtag aacaaatacc taatactttg ttcacctttt atagtggaca cctctcacag 2940
ctttttcagt aagtgatgca attttgtaca tttgtaagat gtgttccaga aaccttttct 3000
cctgcaattc taatgtaccc actcaaactg gtatcaccaa agatctccat ctgattgaaa 3060
aaaagctgcg tgaagtatgc ttatttatgc taaccataca tgatttatac tgttttatag 3120
tacaatgctt atttatgcta accatacata attttattct gttttctagt acattatttg 3180
tgcccctgac cataaatgat cctttctttt acagtggttc cgaagatccc agggggccta 3240
cggtcccagg ttcctaccaa atgaatggga ttatcaatga aacacataat gggaggcatg 3300
cttcagtctc caaggttgtt gagttttgta cggcacttgg tggcaaaaca ccaattcaca 3360
gtgtattagt ggccaacaat ggaatggcag cagctaagtt catgcggagt gtccgaacat 3420
gggctaatga tacttttgga tcagagaagg caattcagct gatagctatg gcaactccgg 3480
aggatctgag gataaatgca gagcacatca gaattgccga tcaatttgta gaggtacctg 3540
gtggaacaaa caacaacaac tatgcaaatg tccaactcat agtggaggtt agttcagctc 3600
atccctcaac acaacatttt cgtttctatt taagttaggg aaaaatctct acgaccctcc 3660
aatttctgaa catccaattt tcaccatcaa ctgcaatcac agatagcaga gagaacaggt 3720
gtttctgctg tttggcctgg ttggggtcat gcatctgaga atcctgaact tccagatgcg 3780
ctgactgcaa aaggaattgt ttttcttggg ccaccagcat catcaatgca tgcattagga 3840
gacaaggttg gctcagctct cattgctcaa gcagctggag ttccaacact tgcttggagt 3900
ggatcacatg tgagccttgt cttctctttt ttagcttatc atcttatctt ttcggtgatg 3960
cattatccca atgacactaa accataggtg gaagttcctc tggagtgttg cttggactca 4020
atacctgatg agatgtatag aaaagcttgt gttactacca cagaggaagc agttgcaagt 4080
tgtcaggtgg ttggttatcc tgccatgatt aaggcatctt ggggtggtgg tggtaaagga 4140
ataaggaagg tttgttcttc ttgtagttat caagagattg tttggattgc aagtgtttag 4200
tgcccatagt taactctggt ctttctaaca tgagtaactc aactttcttg caggttcata 4260
atgatgatga ggttaggaca ttatttaagc aagttcaagg cgaagtacct ggttccccaa 4320
tatttatcat gaggctagct gctcaggtgg ggccttttat ggaagttaca ccttttccct 4380
taatgttgag ttattccgga gttattatgg ttatgttctg tatgtttgat ctgtaaatta 4440
ttgaaattca cctccattgg ttctccagat tagcagacct acaattctac atatggttta 4500
tactttataa atactaggat ttagggatct tcatatagtt tatacatggt atttagattt 4560
catttgtaac cctattgaag acatcctgat tgttgtctta tgtagagtcg acatcttgaa 4620
gttcagttgc tttgtgatca atatggcaac gtagcagcac ttcacagtcg agattgcagt 4680
gtacaacggc gacaccaaaa ggtctgctgt ctcagttaaa tcacccctct gaatgatcta 4740
cttcttgcct gctgcgttgg tcagaggaat aatggttgta ttctactgaa cagataatcg 4800
aggaaggacc agttactgtt gctcctcgtg agactgtgaa agagcttgag caggcagcac 4860
ggaggcttgc taaagctgtg ggttatgttg gtgctgctac tgttgaatac ctttacagca 4920
tggaaactgg tgaatattat tttctggaac ttaatccacg gctacaggtc ggctcctttg 4980
acattcttca ggaattaatt tctgttgacc acatgattta cattgtcaaa tggtctcaca 5040
ggttgagcat cctgtcactg agtggatagc tgaagtaaat ttgcctgcgg ctcaagttgc 5100
tgttggaatg ggtatacccc tttggcagat tccaggtaat gcttcttcat ttagttcctg 5160
ctctttgtta attgaatgag ctcttataca gaccatgaga cacattctac tgttaattca 5220
tagtatcccc tgacttgtta gtgttagaga tacagagatg tatcacaaat tcattgtatc 5280
tcctcaagga ctgtaaaaat cctataatta aatttctgaa aatttgttct tttaagcaga 5340
aaaaaaatct ctaaattatc tccctgtata cagagatcag gcgcttctac ggaatgaacc 5400
atggaggagg ctatgacctt tggaggaaaa cagcagctct agcgactcca tttaactttg 5460
atgaagtaga ttctaaatgg ccaaaaggcc actgcgtagc tgttagaata actagcgagg 5520
atccagatga tgggtttaag cctactggtg gaaaagtaaa ggtgcggttt cctgatgtta 5580
ggtgtatgaa ttgaacacat tgctatattg cagctagtga aatgactgga tcatggttct 5640
cttattttca ggagataagt ttcaagagta aaccaaatgt ttgggcctat ttctcagtaa 5700
aggtagtcct caatattgtt gcactgccac attatttgag ttgtcctaac aattgtgctg 5760
caattgttag ttttcaacta tttgttgttc tgtttggttg actggtaccc tctctttgca 5820
gtctggtgga ggcatccatg aattcgctga ttctcagttc ggtatgtaaa gttaaaagag 5880
taatattgtc tttgctattt atgtttgtcc tcacttttaa aagatattgc cttccattac 5940
aggacatgtt tttgcgtatg gaactactag atcggcagca ataactacca tggctcttgc 6000
actaaaagag gttcaaattc gtggagaaat tcattcaaac gtagactaca cagttgacct 6060
attaaatgta aggactaaat atctgcttat tgaaccttgc tttttggttc cctaatgcca 6120
ttttagtctg gctactgaag aacttatcca tcatgccatt tctgttatct taaattcagg 6180
cctcagattt tagagaaaat aagattcata ctggttggct ggataccagg atagccatgc 6240
gtgttcaagc tgagaggcct ccatggtata tttcagtcgt tggaggggct ttatatgtaa 6300
gacaaactat gccactcatt agcatttatg tgaagcaaat gcggaaaaca tgatcaatat 6360
gtcgtcttat ttaaatttat ttatttttgt gctgcagaaa acagtaactg ccaacacggc 6420
cactgtttct gattatgttg gttatcttac caagggccag attccaccaa aggtactatt 6480
ctgttttttc aggatatgaa tgctgtttga atgtgaaaac cattgaccat aaatccttgt 6540
ttgcagcata tatcccttgt ctatacgact gttgctttga atatagatgg gaaaaaatat 6600
acagtaagtg tgacattctt aatggggaaa cttaatttgt tgtaaataat caatatcata 6660
ttgactcgtg tatgctgcat catagatcga tactgtgagg agtggacatg gtagctacag 6720
attgcgaatg aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct 6780
tttaatgcag gtaatatctt cttcctagtt aaagaagata tatcttgttc aaagaattct 6840
gattattgat cttttaatgt tttcagctgg atggaaacag ccatgtaatt tatgctgaag 6900
aagaggccag tggtacacga cttcttattg atggaaagac atgcatgtta caggtaatga 6960
tagccttgtt ctttttagtt ctagtcacgg tgtttgcttg ctatttgttg tatctattta 7020
atgcattcac taattactat attagtttgc atcatcaagt taaaatggaa cttctttctt 7080
gcagaatgac catgacccat caaagttatt agctgagaca ccatgcaaac ttcttcgttt 7140
cttggttgct gatggtgctc atgttgatgc tgatgtacca tatgcggaag ttgaggttat 7200
gaagatgtgc atgcccctct tatcacccgc ttctggtgtc atacatgttg taatgtctga 7260
gggccaagca atgcaggtac attcctacat tccattcatt gtgctgtgct gacatgaaca 7320
tttcaagtaa atacctgtaa cttgtttatt attctaggct ggtgatctta tagctaggct 7380
ggatcttgat gacccttctg ctgttaagag agctgagccg ttcgaagata cttttccaca 7440
aatgggtctc cctattgctg cttctggcca agttcacaaa ttatgtgctg caagtctgaa 7500
tgcttgtcga atgatccttg cggggtatga gcatgatatt gacaaggtaa acatcatgtc 7560
ctcttgtttt ttcttttgtt tatcatgcat tcttatgttc atcatgtcct ctggcaaatc 7620
tagattccgc tgtcgtttca cacagatttt tctcattctc ataatggtgc caaacataaa 7680
tatgctgcta tattcatcaa tgttttcact cgatttctaa ttttgctttt gagttttaaa 7740
ctttagtaca atccatatct aatctccttt ggcaacagtg aatccattat atatattttt 7800
attaaactgc tttctttttc aggttgtgcc agagttggta tactgcctag acactccgga 7860
gcttcctttc ctgcagtggg aggagcttat gtctgtttta gcaactagac ttccaagaaa 7920
tcttaaaagt gaggtatatt atggttgaca agatagctag tctcatgctc taaggacttg 7980
tacatttcgc cacataggtt aattttccat atcaagttct aatgtacgat ataaaagtag 8040
tactggccta aaacagtatt ggtggttgac tatctttgtt gtgtaagatc aagtatttct 8100
ttttcatgct tagtttgtca atacttcaca tttatcactg acttgtcgag ctaaatgaga 8160
ttttatttga tttctgtgct ccattatttt tgtatatata tatatatatt taactatgac 8220
tatatgttat gcctcaaacg tttcaaactc tttcagttgg agggcaaata tgaggaatac 8280
aaagtaaaat ttgactctgg gataatcaat gatttccctg ccaatatgct acgagtgata 8340
attgaggtca gttattcaat ttgttgtgat aatcactgcc ttaactgttc gttcttttaa 8400
caagcggttt tataggaaaa tcttgcatgt ggttctgaga aggagaaggc tacaaatgag 8460
aggcttgttg agcctcttat gagcctactg aagtcatatg agggtgggag agaaagtcat 8520
gctcactttg ttgtcaagtc cctttttgag gagtatctct atgttgaaga attgttcagt 8580
gatggaattc aggttaactt acctattcgc attaaacaaa tcatcagttg ttttatgata 8640
aagtcaaaat gtttatattt cccattcttc tgtggatcaa atatatcacg gacatgatat 8700
agtttcctta ggctatataa tggttcttca tcaaataata ttgcaggaaa cagtatagca 8760
aactatttgt atatactcga gatggaaatt gttagaaaca tcattgacta aatctgtcct 8820
ttgttacgct gtttttgtag tctgatgtga ttgagcgtct gcgccttcaa catagtaaag 8880
acctacagaa ggtcgtagac attgtgttgt cccaccaggt aaatttcttc atggtctgat 8940
gacttcactg cgaatggtta ctgaactgtc ttcttgttct gacaatgtga cttttctttg 9000
tagagtgtta gaaataaaac taagctgata ctaaaactca tggagagtct ggtctatcca 9060
aatcctgctg cctacaggga tcaattgatt cgcttttctt cccttaatca caaagcgtat 9120
tacaaggtga ccaggataaa cataaataaa cgtgaatttt tcaatgacct tttcttctga 9180
catctgaatc tgatgaattt cttgcatatt aatacagttg gcacttaaag ctagtgaact 9240
tcttgaacaa acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct 9300
ggagatgttt actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa 9360
ggagagcatg gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt 9420
atttgattgt agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt 9480
ataccaggta tgagaagaaa gaccttttga aattatttat attaacatat cctagtaaaa 9540
cagcatgctc atcatttctt aaaaaaagtt tacagcacct gatgtttggt tactgaccgc 9600
atcattaaaa taaagttact tgttgtggag agatgtattt tggaacttgt ggcacatgca 9660
gtaacatgct actgctcgat atgtttgcta acttgacaac aatatttttc agcctcatct 9720
tgtaaaggac agtatcaaaa tgaaatggat agaatcgggt gttattgctt tatgggaatt 9780
tcctgaaggg cattttgatg caagaaatgg aggagcggtt cttggtgaca aaagatgggg 9840
tgccatggtc attgtcaagt ctcttgaatc actttcaatg gccattagat ttgcactaaa 9900
ggagacatca cactacacta gctctgaggg caatatgatg catattgctt tgttgggtgc 9960
tgataataag atgcatataa ttcaagaaag gtatgttcat atgctatgtt ggtgctgaaa 10020
tagttatata tgtagttagc tggtggagtt ctggtaatta acctatccca ttgttcagtg 10080
gtgatgatgc tgacagaata gccaaacttc ccttgatact aaaggataat gtaaccgatc 10140
tgcatgcctc tggtgtgaaa acaataagtt tcattgttca aagagatgaa gcacggatga 10200
caatgcgtcg taccttcctt tggtctgatg aaaagctttc ttatgaggaa gagccaattc 10260
tccggcatgt ggaacctcct ctttctgcac ttcttgagtt ggtacgtgat atcatcaaaa 10320
tgataatgtt ttggtatggc attgattatc ttctatgctc tttgtattta ttcagcctat 10380
tgtggataca ggacaagttg aaagtgaaag gatacaatga aatgaagtat accccatcac 10440
gggatcgtca atggcatatc tacacactta gaaatactga aaaccccaaa atgttgcacc 10500
gggtattttt ccgaaccctt gtcaggcaac ccagtgtatc caacaagttt tcttcgggcc 10560
agattggtga catggaagtt gggagtgctg aagaacctct gtcatttaca tcaaccagca 10620
tattaagatc tttgatgact gctatagagg aattggagct tcacgcaatt agaactggcc 10680
attcacacat gtatttgcat gtattgaaag aacaaaagct tcttgatctt gttccagttt 10740
cagggtaagt gcgcatattt ctttttggga acatatgctt gcttatgagg ttggtcttct 10800
caatgatctt cttatcttac tcaggaatac agttttggat gttggtcaag atgaagctac 10860
tgcatattca cttttaaaag aaatggctat gaagatacat gaacttgttg gtgcaagaat 10920
gcaccatctt tctgtatgcc aatgggaagt gaaacttaag ttggactgcg atggtcctgc 10980
cagtggtacc tggaggattg taacaaccaa tgttactagt cacacttgca ctgtggatgt 11040
aagtttaatc ctctagcatt ttgttttctt tggaaaagca tgtgatttta agccggctgg 11100
tcctcatacc cagacctagt gatctttata tagtgtagac atttttctaa ctgcttttaa 11160
ttgttttaga tctaccgtga gatggaagat aaagaatcac ggaagttagt ataccatccc 11220
gccactccgg cggctggtcc tctgcatggt gtggcactga ataatccata tcagcctttg 11280
agtgtcattg atctcaaacg ctgttctgct aggaataata gaactacata ctgctatgat 11340
tttccactgg tgagttgact gctcccttat attcaatgca ttaccatagc aaattcatat 11400
tcgttcatgt tgtcaaaata agccgatgaa aattcaaaac tgtaggcatt tgaaactgca 11460
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 11520
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 11580
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 11640
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 11700
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 11760
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 11820
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 11880
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 11940
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 12000
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 12060
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 12120
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 12180
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 12240
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 12300
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 12360
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 12420
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 12480
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 12540
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 12600
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 12660
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 12720
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 12780
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 12840
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 12900
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 12960
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 13020
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 13080
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 13140
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 13200
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 13260
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 13320
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 13380
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 13440
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 13500
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 13560
cagggtcttt ccatgttact agataaggta attagcttac tgatgcttat ataaattctt 13620
tttcattaca tatggctgga gaactatcta atcaaataat gattataatt ccaatcgttc 13680
tttttatgcc attatgatct tctgaaattt ccttctttgg acacttattc agatggatcc 13740
ctctagaaga gctcaacttg ttgaagaaat caggaaggtc cttggttgaa tcatatgatg 13800
ccaaaactat tattggaggc acaaatagct tgtggaccct gtcggattgt tggtgagtgt 13860
atattggatt tgttagttct gccagatgaa agtgcaagtc tgatgattca tgataccgtc 13920
agttggcaag aacaccggtt aacctgagtg cttgtttaca aatggtcctt tatgacaatc 13980
gttgtttcgc gctagttccg tgatctacta tcatctgtta gacgctgtaa ttagtgagtc 14040
tccgcggatc cacagtatac ggttgagctg ttgattcaat tttggacacg aataatatga 14100
ttttgtaggc ataaatgcgt ctgtatgtga aataaattgt ctgttgagtt aacacacaag 14160
atgacaatat gtttgtgctc tactgctatt gtccatgaat actgattgcg gaatcaacca 14220
catgcattat acccatctca acgtgaccag tgattggacg gatgaccaaa gtggagcggt 14280
tggatggaag aaactatatt tatgaacaag tttaattctc agccgaccta tggccaaaca 14340
tgcggatgag aacaatccaa atgaggcttt tgagggtagc aaaggatatg gaaaataaac 14400
aaattacaga aagctcaaaa aagtcgctta aggtttgttc agattgatgc tattttcaat 14460
catactattt tttggcaaag ttgctaaaaa aaattagttt gttgctaaat ttggtaaata 14520
ttctactaaa a 14531
<210> 2
<211> 6984
<212> DNA
<213> Oryza sativa
<400> 2
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 3
<211> 2327
<212> PRT
<213> Oryza sativa
<400> 3
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 4
<211> 6984
<212> DNA
<213> Oryza sativa
<400> 4
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag agtatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 5
<211> 2327
<212> PRT
<213> Oryza sativa
<400> 5
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Ser Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 6
<211> 6984
<212> DNA
<213> Oryza sativa
<400> 6
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatttacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 7
<211> 2327
<212> PRT
<213> Oryza sativa
<400> 7
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Leu
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 8
<211> 6984
<212> DNA
<213> Oryza sativa
<400> 8
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaaag caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 9
<211> 2327
<212> PRT
<213> Oryza sativa
<400> 9
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Ser Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 10
<211> 6984
<212> DNA
<213> Oryza sativa
<400> 10
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatttacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaaag caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 11
<211> 2327
<212> PRT
<213> Oryza sativa
<400> 11
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Leu
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Ser Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 12
<211> 6984
<212> DNA
<213> Oryza sativa
<400> 12
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag agtttacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 13
<211> 2327
<212> PRT
<213> Oryza sativa
<400> 13
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Ser Leu
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 14
<211> 6984
<212> DNA
<213> Oryza sativa
<400> 14
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag agtatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaaag caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 15
<211> 2327
<212> PRT
<213> Oryza sativa
<400> 15
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Ser Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Ser Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325

Claims (20)

1.一种具有除草剂抗性的ACCase突变蛋白，其特征在于所述ACCase突变蛋白相较于野生型ACCase具有一个或多个氨基酸差异位点，所述氨基酸差异中的至少一个为相应于野生型ACCase蛋白的I1792、G2107、N1791及其组合的位置处的氨基酸差异。
2.根据权利要求1所述，更具体的，ACCase突变蛋白的氨基酸差异位点为I1792L和/或G2107S和/或N1791S突变位点。
3.根据权利要求1和2所述的ACCase突变蛋白，其特征在于所述突变蛋白的氨基酸序列如SEQ ID NO：5、7、9、11、13或15所示。
4.根据权利要求1~3所述的ACCase突变蛋白，其特征在于所述编码该ACCase突变蛋白的核苷酸序列如SEQ ID NO：4、6、8、10、12或14所示。
5.根据权利要求1所述，其中除草剂是指ACCase抑制剂类除草剂，优选的是烯草酮。
6.一种表达盒、载体或细胞，其包含的ACCase编码区的核苷酸序列具有一个或多个A5372、A5374、G6319及其组合的位置处的突变位点，或是含有核苷酸序列SEQ ID NO：4、SEQID NO：6、SEQ ID NO：8、SEQ ID NO：10、SEQ ID NO：12或SEQ ID NO：14之一，或是可编码氨基酸序列如SEQ ID NO：5、SEQ ID NO：7、SEQ ID NO：9、SEQ ID NO：11、SEQ ID NO：13或SEQID NO：15所示的核苷酸。
7.一种米制品，其包含权利要求4所述的核酸，或者其表达权利要求1~3之任一所述的蛋白。
8.权利要求1~3之任一所述的蛋白质、权利要求4所述的核苷酸、权利要求6所述的表达盒、载体或细胞以及权利要求7所述的米制品，在获得具有除草剂抗性的植物中的应用，其中优选除草剂是ACCase抑制剂类除草剂，更优选的是烯草酮。
9.一种使植物获得除草剂抗性的方法，其特征在于所述除草剂抗性可以通过以下方法之一获得：
a）通过突变目标植株内源的ACCase基因的5372和/或5374和/或6319位点，从而使编码蛋白质的1792和/或2107和/或1791位点突变，进而使该植物体产生对ACCase抑制剂类除草剂的抗性；或
b）将具有除草剂抗性的ACCase突变体核苷酸序列，通过转基因的方法转入目标植株，使目标植株获得对ACCase抑制剂类除草剂的抗性，其中所述核苷酸序列如SEQ ID NO：4、6、8、10、12或14所示，或可编码氨基酸序列如SEQ ID NO：5、7、9、11、13或15所示的核苷酸；或
c）通过杂交、转育、回交等方法，将含有权利要求1~3所示的ACCase突变蛋白的ACCase突变植株中的突变位点转移到目标植株中，使目标植株获得对ACCase抑制剂类除草剂的抗性，其中所述的ACCase突变蛋白相较于野生型ACCase蛋白至少含有一个I1792、G2107、N1791S及其组合的位置处的突变位点。
10.根据权利要求9所述的方法，其中所述的突变选自下列方法之一：物理或化学方法导致的基因突变，化学方法包括用EMS等诱变剂处理所导致的诱变，所述突变还可以是点突变，也可以是DNA缺失或插入突变，还可以是通过RNAi、基因定点突变等基因沉默手段产生，所述基因定点突变方法包括但不限于ZFN定点突变方法、TALEN定点突变方法、和/或CRISPR-Cas9等基因组定点突变方法。
11.根据权利要求9所述的方法，其中所述的除草剂是指ACCase抑制剂类除草剂，优选的是烯草酮。
12.根据权利要求9所述的方法，其中所述的植物优选为禾本科植物，更优选的为水稻、玉米、小麦、大麦、高粱、黍、谷子等。
13.一种ACCase抑制剂类除草剂抗性植株的应用，所述除草剂抗性植株可以应用于杂交育种提纯、水稻直播、防除植物落田谷、防除田间抗性杂草等方面，其特征在于，所述ACCase抑制剂类除草剂抗性植株的ACCase蛋白相较于野生型ACCase具有至少一个I1792、G2107、N1791及其组合的位置处的氨基酸差异，更具体的，含有至少一个I1792L、G2107S、N1791S及其组合的突变位点。
14.一种防除田间抗性杂草的方法，其包括：
a）使目标植物获得权利要求9所提及的ACCase抑制剂类除草剂抗性；
b）获得该除草剂抗性植株后，将其种子播到田中；和
c）对所述植物和其附近的杂草施用多种除草剂混合物，从而控制所述植物附近的杂草。
15.根据权利要求14所述的应用，其中多种除草剂混合物包括至少一种ACCase抑制剂类除草剂，更具体的包括以下至少一种：氰氟草酯、高氟吡禾灵、精氟吡禾草灵、喹禾糠酯、禾草灭、烯禾啶、噻草酮、烯草酮、苯草酮等。
16.一种防除水稻落田谷的方法，其包括：
a）使目标植物获得权利要求9所提及的ACCase抑制剂类除草剂抗性；
b）获得该除草剂抗性植株后，将其种子播到田中；和
c）在植物幼苗期，用ACCase抑制剂类除草剂或含有ACCase抑制剂类除草剂的混剂进行喷施，从而使上一季水稻收割后掉落在田间的不具有ACCase抑制剂类除草剂抗性的种子生长出的幼苗死亡，而具有ACCase抑制剂类除草剂抗性的目标植株健康成长。
17.一种适于水稻直播的方法，其包括：
a）使用于直播的水稻品种获得权利要求9所提及的ACCase抑制剂类除草剂抗性；
b）获得该除草剂抗性植株后，将其种子播到田中；和
c）在植株幼苗期，用ACCase抑制剂类除草剂或含有ACCase抑制剂类除草剂的混剂进行喷施，使田中的杂草或杂草稻死亡，而具有ACCase抑制剂类除草剂抗性的目标植株健康成长。
18.根据权利要求14、16和17所述的应用，其特征在于所述ACCase基因突变后的蛋白氨基酸序列如SEQ ID NO：5、7、9、11、13或15所示。
19.根据权利要求18所述的应用，其特征在于所述编码该ACCase突变蛋白的核苷酸序列如SEQ ID NO：4、6、8、10、12或14所示。
20.根据权利要求14、16和17所述的应用，其特征在于所述目标植物优选为禾本科植物，更优选的为水稻、玉米、小麦、大麦、高粱、黍、谷子等。
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CN109371000A (zh) * 2018-10-10 2019-02-22 江苏省农业科学院 一种水稻ACCase突变型基因及其在植物抗除草剂中的应用
CN109371000B (zh) * 2018-10-10 2021-10-26 江苏省农业科学院 一种水稻ACCase突变型基因及其在植物抗除草剂中的应用
CN113817691A (zh) * 2018-11-28 2021-12-21 江苏省农业科学院 一种小麦ACCase突变型基因及其在植物抗除草剂中的应用
CN113817691B (zh) * 2018-11-28 2024-04-26 江苏省农业科学院 一种小麦ACCase突变型基因及其在植物抗除草剂中的应用
CN112824526A (zh) * 2019-11-18 2021-05-21 安徽省农业科学院水稻研究所 一种水稻ACCase突变型蛋白及相应基因
CN113005103A (zh) * 2019-12-21 2021-06-22 科稷达隆生物技术有限公司 一组抗除草剂蛋白及其在植物育种中的应用
CN113755457A (zh) * 2020-09-23 2021-12-07 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用
WO2022063136A1 (zh) * 2020-09-23 2022-03-31 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用
CN114032218A (zh) * 2021-02-08 2022-02-11 山东舜丰生物科技有限公司 新型抗除草剂乙酰辅酶a羧化酶突变体及其应用
CN113073088A (zh) * 2021-03-31 2021-07-06 四川天豫兴禾生物科技有限公司 具有三酮类除草剂抗性的hir突变体及其在植物育种中的应用

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