CN113005103A - 一组抗除草剂蛋白及其在植物育种中的应用 - Google Patents

一组抗除草剂蛋白及其在植物育种中的应用 Download PDF

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CN113005103A
CN113005103A CN201911330009.8A CN201911330009A CN113005103A CN 113005103 A CN113005103 A CN 113005103A CN 201911330009 A CN201911330009 A CN 201911330009A CN 113005103 A CN113005103 A CN 113005103A
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李雪峰
罗祖勇
张李
王明
李莹莹
李相敢
黎跃进
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Keji Dalong Biotechnology Co ltd
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Abstract

本发明公开了一组抗除草剂蛋白及其在植物育种中的应用。提供了使植物具有除草剂抗性的ACCase蛋白质，其与野生型相比，带有在位点2008从A到V的氨基酸突变。另外，本发明还提供了相应的获得具有除草剂抗性的植物的方法和应用、以及鉴定本发明的抗除草剂植物或抗除草剂植物的方法。

Description

一组抗除草剂蛋白及其在植物育种中的应用
技术领域
本发明属于植物蛋白质领域，具体而言，本发明涉及能赋予高粱或者其他植物除草剂抗性(耐受性)的蛋白及其在植物育种中的应用。
背景技术
除草剂又称杀草剂。能杀死杂草或有害植物，而不影响农作物正常生长的化学药剂。可用于防治农田杂草或杀灭非农耕地的杂草或灌木。按其作用特点可分为选择性除草剂和灭生性除草剂。选择性除草剂指在使用一定剂量和浓度的范围内，可有选择性地杀死某些种类的植物，而对其他种类植物无害。然而，由于灭生性除草剂通常也能杀死农作物本身，因此对一般不具有除草剂抗性(耐受性)的农作物而言，除草剂的使用在时间和空间上受到了极大限制，如需要在农作物播种前一段时间使用除草剂，以达到除草的目的。传统的清除杂草方式是人工操作，所需要消耗的人力资源和成本非常高，不利于农业大规模的生产。培育对除草剂有抗性的农作物有极大的经济价值和商业用途，这样可以在种植期间使用除草剂，对抗性农作物使用对应的除草剂，在不影响农作物生长的情况下，杀死杂草，不但拓宽除草剂的使用范围，也可以节约农业的人力资源和成本，有利于农业的大规模的生产。
乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACCase)，是植物中脂肪酸合成途径中的关键酶之一。通过抑制ACCase即可抑制植物脂肪酸合成，最终造成植株死亡达到杀死植物的目的。因此，农药公司以ACCase为靶标，开发研制了一系列的除草剂。在市场上的这些除草剂可大致分为三类，即芳氧苯氧丙酸酯类、环己二酮类和苯基吡唑啉类。(王俊丽,徐小博, 孙玥,朱晔荣,王勇,刘学军.乙酰辅酶A羧化酶抑制剂类除草剂的研究进展ResearchProgress on Acetyl-CoA Carboxylase Inhibitor Herbicides[J].植物学研究,2019,08(05):410-415.)因此，在本文中，除草剂抗性指的是对上述三大类的除草剂具有耐受性。而植物对这类除草剂的抗性则来自ACCase基因一个或多个氨基酸突变而导致酶的结构变化。
本发明人正是长期而艰苦的研究实践，通过化学诱变的方法在高粱突变植株中发现了一系列(突变)蛋白，其能够在除草剂存在的条件下基本保持原有蛋白的正常植物生理功能，从而使得植物具有除草剂抗性(耐受性)。本发明人还开发了这些蛋白及其编码基因在转基因，基因编辑或者非转基因，非基因编辑植物育种中的应用，可用于培育具有除草剂抗性的植物，尤其是农作物，如其他农业植物等。
发明内容
本发明的目的在于提供一个或者多个新的使植物具有除草剂抗性的氨基酸序列，本领域人员可通过已知的生物技术，例如转基因，基因编辑或者突变技术，使得植物能够表达上述的蛋白质，该植物既可具有除草剂的抗性。
具体而言，在所述目的方面，本发明提供了使植物具有除草剂抗性的ACCase的氨基酸序列。ACCase就是乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACCase)的简称,其酶分类编号为EC 6.4.1.2。使植物具有除草剂抗性的ACCase的氨基酸序列与野生型相比，其在位点2008 上，带有从A到V的氨基酸突变。野生型高粱的ACCase的氨基酸基因序列如SEQID NO： 2所述，突变后的ACCase的氨基酸序列如SEQ ID NO：4所述。
本发明的另一个目的在于提供具有除草剂抗性的x1)植物；x2)单子叶植物或双子叶植物；x3)高粱。示例性的植物包括水稻、玉米、小麦、黑麦、燕麦、大麦、大豆、马铃薯、油菜、甜菜、甘蔗、高粱、西红柿、南瓜、辣椒、生菜、向日葵、咖啡、茶、棉花、苜蓿、烟草、或拟南芥等。
本发明的另一个目的在于提供获得具有除草剂抗性的x1)植物；x2)单子叶植物或双子叶植物；x3)高粱的方法。示例性的植物包括水稻、玉米、小麦、黑麦、燕麦、大麦、大豆、马铃薯、油菜、甜菜、甘蔗、高粱、西红柿、南瓜、辣椒、生菜、向日葵、咖啡、茶、棉花、苜蓿、烟草、或拟南芥等。上述除草剂抗性植物的获得的方法是通过生物技术，例如转基因，基因编辑，或者突变技术，例如化学诱变等或者通过传统育种，例如将已有除草剂抗性植物与其他植物品种杂交产生的植物后代、以及回交或自交产生的植物后代等等的方法，将上述氨基酸突变在植物中表达，从而使植物获得除草剂的抗性。
本发明的另一个目的在于提供上述基因在x1)或x2)或x3)中获得除草剂抗性的应用:x1) 植物；x2)单子叶植物或双子叶植物；x3)高粱。示例性的植物包括水稻、玉米、小麦、黑麦、燕麦、大麦、大豆、马铃薯、油菜、甜菜、甘蔗、高粱、西红柿、南瓜、辣椒、生菜、向日葵、咖啡、茶、棉花、苜蓿、烟草、或拟南芥等。该应用包括，通过生物技术，突变技术或者传统育种技术获得具有除草剂抗性的植物过程中的应用，例如转基因植物中的应用，基因编辑植物中的应用或者非转基因植物，非基因编辑植物中的应用等等。
本发明的另一个目的在于提供一个鉴定方法，本领域人员可通过已知的生物技术，通过检测是否在植物中是否含有上述的突变后的氨基酸序列，来判断植物是否采用本发明方法获得的植物的鉴定方法。测定的步骤可以通过常规的核酸检测和/或蛋白质检测方法来进行，只需要能够检测出蛋白质带有A2008V突变或其相应核酸突变的方法都可以。
下面将通过具体的附图和实施例对本发明进行详细地描述，使本发明更容易理解。需要特别指出的是，这些描述仅仅是示例性的描述，并不构成对本发明范围的限制。而在阅读本说明书之后，在不脱离本发明的目的和/或所附的权利要求书的范围的前提下，本领域技术人员可以提出本发明的其它实施方式，修改和等同物。依据本说明书的论述，本发明的许多变化、改变对所属领域技术人员来说都是显而易见的了。
附图说明
图1：含有A2008V突变体植株与野生型植株在喷洒除草剂后的14天在大田里的对比照片；
图2：含有A2008V突变体植株与野生型植株在喷洒除草剂后的12天在温室里的对比照片。
具体实施方式
下述实施例子中所用方法如无特殊说明，均为本领域人员常用的方法。
实施例1：从高粱EMS诱变库M2代群体中筛选抗性植株
为了对高粱突变体库进行大规模筛选，我们通过化学诱变的方法构建高粱突变体库。具体步骤如下：称取大约1kg的高粱种子，装入80目的网袋中，共计10份，自来水冲洗2次。然后用0.3％(w/w)甲磺酸乙酯(EMS)浸泡高粱种子12h，期间每1h摇动一次种子。12h 后取出种子，置于自来水中浸洗5次，EMS废液及浸洗废液收集起来专门容器保存。将浸洗后的种子摊开风干，之后即刻安排播种。同时，光照培养箱测试处理后的种子芽率，控制发芽率在65％左右。待植株成熟后分地块收获，每个地块面积1亩。地块内分大中小穗3种类型，分别混收，晾干至含水量13％。将收获的种子按1万粒/m2的密度播种，待幼苗长至3～ 4叶期时，用芳氧苯氧丙酸酯类除草剂“喹禾糠酯”(CAS号119738-06-6)喷雾处理，4％水剂，喷施剂量160ml/亩、兑水30L。2周后调查幼苗枯死情况，发现其中有绿色存活小苗，保留并繁殖这些绿色小苗。
实施例2：筛选出的抗除草剂高粱的分子检测
对筛选得到的2个疑似抗除草剂高粱的单株进行了分子检测。具体步骤如下：取可能带有除草剂抗性的植株叶片10-20mg，放入离心管中，加入钢珠后，置于液氮速冷，用组织研磨机将叶片充分震荡研磨至粉末状，采用SDS法提取样品DNA，然后用特异性引物进行PCR测序。测序公司：英潍捷基(上海)贸易有限公司。经过测序公司确认：高粱中编码了ACCase基因中的一个突变位点，第6023位C-T的碱基突变，对应的是第2008位A-V的氨基酸的突变；野生型植株的核酸序列如SEQ ID NO：1所示，野生型植株的氨基酸序列如SEQ ID NO： 2所示；第6023位C-T的碱基突变后的核酸序列如SEQ ID NO：3所示，第2008位A-V的碱基突变后的氨基酸序列如SEQ ID NO：4所示。
实施例3：筛选出的抗除草剂植株后代的除草剂耐受性的田间试验
将鉴定为突变体的株系后代单株收种，在田间按50×20cm行株距、5m行长小区种植，野生型作为对照。待高粱长至四叶期时，按照48g ai/ha的剂量喷施喹禾糠酯。喷施14、21 天后调查植株枯死情况，发现野生型对照在喷施喹禾糠酯之后几乎停止生长，叶片枯黄或枯死，而筛选到的抗除草剂阳性苗则呈现对喹禾糠酯完全耐受的表型，其正常的生长发育没有受到影响，最终正常拔节抽穗。这表明我们筛选到的抗除草剂突变体能够稳定地遗传其对芳氧苯氧丙酸酯类除草剂的抗性(如图1)。
实施例4：筛选出的抗除草剂高粱的后代的除草剂耐受性的温室实验
将实施例3中的抗除草剂突变体留种进行后代的除草剂耐受的实验。温室除草剂测试步骤分为以下几个部分：
(1)将营养土铺在穴盘上，穴盘至于盛水的托盘上，让营养土充分吸水。
(2)实施例3收获的高粱种子播种于穴盘的营养土里，再覆一层约1cm的营养土。
(3)人工气候室培养，设置温度为22-26℃，湿度为70％，光照与暗培养分别为14小时和10小时。
(4)出苗后适当间苗，间掉不正常苗，每穴保留4株。
(5)待幼苗长至3-4叶期时，喷施除草剂喹禾糠酯。除草剂剂量为48g ai/ha，使用扇形喷头、喷施柜喷施。
(6)除草剂喷施后14天调查和记录成苗率、植株鲜重、药害症状(如图2)。
序列表
<110> 科稷达隆生物技术有限公司
<120> 一组抗除草剂蛋白及其在植物育种中的应用
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ggaatggcag cagcaaagtt catgaggagt gtccggacat gggctaatga tacttttgga 480
tctgagaagg caattcagct catagctatg gcaactccgg aagacatgag gataaatgca 540
gagcacatta gaattgctga tcaatttgta gaggtgcctg gtggaacaaa caataataac 600
tacgccaacg ttcaactcat agtggaggta gcagaaagag taggtgtttc tgctgtttgg 660
cctggttggg gtcatgcttc tgagaatcct gaactgccgg atgcattgac tgcaaaagga 720
atcgttttcc ttgggccacc tgcatcatca atgaatgcat tgggagataa ggtcggttca 780
gctctcattg ctcaagcagc tggggtccca actcttgctt ggagtggatc acatgttgaa 840
gtgccattag agtgctgctt agacgctata cctgaggaga tgtatagaaa agcttgtgtt 900
actaccacag aggaagcagt tgcaagttgt caagtgattg gttatcctgc catgattaag 960
gcatcctggg gaggtggtgg taaaggaata agaaaggttc ataatgatga tgaggttaga 1020
gcactgttta agcaagtaca aggtgaagtc cctggctccc caatatttat catgaggctt 1080
gcagcccaga gtcggcatct tgaagttcag ttgctttgtg atcaatatgg caatgtagca 1140
gcacttcaca gtcgtgattg cagtgtgcaa cggcgacacc agaagattat tgaagaaggc 1200
ccagttactg ttgctcctcg tgagacagtt aaagcacttg agcaggcagc aaggaggctt 1260
gctaaggctg tgggttatgt tggtgctgct actgttgagt atctttacag catggaaact 1320
ggagaatact attttctgga gcttaatccc cgactacagg tcgagcatcc agtcaccgaa 1380
tggatagctg aagtaaatct gcctgcagct caagttgctg ttggaatggg catacctctt 1440
tggcaaattc cagaaatcag acgtttctat ggaatggact atggaggagg gtatgatatt 1500
tggaggaaaa cagcagctct tgctacacca tttaattttg atgaagtaga ttctcaatgg 1560
ccaaagggcc attgtgtagc agttagaatt actagtgagg accctgatga tggtttcaaa 1620
cctactggtg ggaaagtgaa ggagataact tttaaagcca agcctaatgt ttgggcctac 1680
ttctcagtaa agtctggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740
tttgcatatg gactctctag atcagcagca ataacaaaca tggctcttgc attaaaagag 1800
attcaaattc gtggagaaat tcattcaaat gttgattaca ctgttgacct cttaaacgct 1860
tcagacttca gagaaaacaa gattcatact ggttggctgg ataccagaat agctatgcgt 1920
gttcaagctg agaggccccc atggtatatt tcagtggttg gaggtgcttt atacaaaaca 1980
gtaaccacca atgcagccac tgtttctgac tatgttagtt atctcaccaa gggccagatt 2040
ccaccaaagc atatatccct tgtcaattct acagttagct tgaatataga agggagcaaa 2100
tacacaattg aaactgtaag gactggacat ggtagctaca ggttgcgaat gaatgaatcg 2160
acagttgaag cgaatgtaca atctttatgt gatggtggac tcttaatgca gttggatgga 2220
aacagccatg taatttatgc agaagaagaa gctggtggta cacggcttca gattgatgga 2280
aagacatgct tgttgcagaa tgaccatgat ccatcaaagt tattagctga gacaccctgc 2340
aaacttcttc gtttcttggt tgctgatggt gctcatgttg atgcggatgt accatacgct 2400
gaagttgagg ttatgaagat gtgcatgcct ctcctgtcac ctgcttctgg tgtcgttcat 2460
tgtatgatgt ctgagggcca ggcgttgcag gctggtgatc ttatagcaag gctggatctt 2520
gatgaccctt ctgctgtgaa aagggctgaa ccatttgatg gaatatttcc acaaatggcg 2580
ctccctgttg ctgcctctag tcaagtacac aaaagatatg ctgcaagttt gaatgctgct 2640
cgaatggttc ttgcaggata tgagcacaat atcaatgaag tcgttcaaga tttgatatgc 2700
tgcctggaca accctgagct tcctttccta cagtgggatg aacttatgtc tgttctagca 2760
acgaggcttc caagaaatct caagagtgag ttagaggata aatacaagga atacaagttg 2820
aatttttacc gtggaaaaaa cgtggacttc ccttccaagt tgctaagaga catcattgag 2880
gaaaatcttg catatggttc agagaaggaa aaggctacaa atgagaggct tgttgagcct 2940
cttacgaacc tactgaagtc atatgagggt gggagagaaa gtcatgcaca ttttattgcc 3000
aagtcccttt tcgaggagta tcttatggtg gaagaacttt tcagtgatgg aattcagtct 3060
gacgttattg aaaccttgcg tcatcagcac agtaaagacc tgcagaaggt tgtagacatt 3120
gtgttgtctc accagggtgt gagaaacaaa gctaagcttg taacagcact catggaaaag 3180
ctggtttatc caaatccggt tgcttacagg gatctgttgg ttcgcttttc ttccctcaat 3240
cataaaagat attataagtt ggcccttaaa gcaagtgaac ttcttgaaca aaccaaacta 3300
agtgaactcc gtgcaagcat tgcaagaagc ctttcagatc tggggatgca taagggagac 3360
atgactatta aggatagcat ggaagattta gtctctgccc cattgcctgt tgaagatgct 3420
cttatttctt tgtttgatta cagcgatgca actgttcagc agaaagtgat tgagacatac 3480
atatcacgat tgtaccagcc tcatcttgtg aaggatagca tccaaatgaa attcaaggaa 3540
tatggtgcta ttgctttttg ggaattttct gaagggcatg ttgatacttc aaatggacat 3600
gggactattc ttggtgggaa gagatggggt tccatggtcg tcctcaaatc acttgaatct 3660
gcatcaacag ccattgtggc tgcattaaag gattcagcac agtacaacag ctctgagggc 3720
aacacgatgc acattgcatt attgagtgct gaaaatgaaa gtaatatgag tggaacaagt 3780
gatgatcaag ctcaacatag gatggaaaag cttaccaaga tactgaagga tactaatgtt 3840
gcaagtgatc tccgagctgc tggtttgaag gttataagtt gcattgttca aagagatgaa 3900
gctcgcatgc caatgcgcca cacattcctc tggttggatg aaaagagttg ttatgaagaa 3960
gagcaaattc tccggcatgt ggagcctccc ctctctgcac ttcttgaatt ggataagttg 4020
aaagtgaaag gatacaatga aatgaagtat actccgtcac gtgatcgtca atggcatatc 4080
tacacactaa gaaatactga aaaccccaaa atgttgcata gggtattttt ccgaactatt 4140
gtcaggcaac ccaatgcagg caacaagttt acatcagctc aggtcagtga tactgaagta 4200
ggaggtcctg aagattctct gtcattcaca tcgaatagca tcctaagatc attgatgact 4260
gctatagaag aattagagct tcatgcgatt aggacaggtc attctcacat gtatttgtgc 4320
atactgaaag agcaaaagct tcttgatctc attccatttt cagggagtac aattgttgat 4380
gttggccaag atgaagctac tgcttgttca cttttaaaat caatggcttt gaagatacat 4440
gaccttgttg gtgcaaggat gcatcatctg tctgtatgcc agtgggaggt gaaactcaag 4500
ttggattgtg atgggcctgc aagtggtacc tggagagttg tgactacaaa tgttactagt 4560
cacacctgca ccattgatat ctaccgagaa gtggaagata cagaatcgca gaagttagta 4620
taccattcaa ccacttcggc agctggtcct ggtccgttgc atggtgttgc actgaataat 4680
ccatatcaac ctttaagtgt gattgatcta aagcgctgct ctgctaggaa caacagaaca 4740
acatattgct acgattttcc gctggcattt gaaactgcac tgcagaagtc atggcagtcc 4800
aacggctcta gtgtttctgt aggcagtgga aatagtaaat cctacgtgaa ggcaactgag 4860
ctggtgtttg ctgaaaaaca tgggtcctgg ggcactccta tagttcccat ggaacgtcct 4920
gctgggctca atgacattgg tatggtcgct tggatcttgg agatgtcaac acctgaattt 4980
cccaatggca ggcagattat tgttgtagca aatgatatta ctttcagagc tggatcattc 5040
ggcccaaggg aagatgcatt ttttgaagct gtcaccaacc tggcttgtga aaggaaactt 5100
ccccttatat acttggcagc aaactctggt gctaggattg gcatagctga tgaagtaaaa 5160
tcttgcttcc gtgttgggtg gtctgacgaa ggcagccctg agcgagggtt tcagtacatc 5220
tatctgactg aagaagacta tgcccgtatt agctcttctg ttatagcaca taagctgcag 5280
ctagatagcg gtgaaattag gtggattatt gactctgttg tgggcaagga ggatgggctt 5340
ggtgttgaga acatacatgg aagtgctgct atcgccagtg cttattctag ggcatatgag 5400
gagacattta cacttacatt tgtgaccgga cggactgtag gaataggagc ttatcttgct 5460
agacttggta tacggtgcat acagcgtctt gaccagccga ttattttaac agggttttct 5520
gccctgaaca agctccttgg gcgggaagtg tacagctccc acatgcagct tggtggtcct 5580
aagatcatgg cgaccaatgg tgttgtccac ctgactgttc cagatgacct tgaaggtgtt 5640
tccaatatat tgaggtggct cagctatgtt cctgcaaaca ttggtggacc tcttcctatt 5700
accaaacctt tggaccctcc agacagacct gttgcataca tccctgagaa cacatgcgat 5760
ccacgtgcag ccatccgtgg tgtagatgac agccaaggga aatggttggg tggtatgttt 5820
gacaaagaca gctttgtgga gacatttgaa ggatgggcaa aaacagtggt tactggcaga 5880
gcaaagcttg gaggaattcc tgtgggtgtc atagctgtgg agacacagac catgatgcag 5940
cttgtccctg ctgatccagg tcagcttgat tcccatgagc gatccgttcc tcgggctgga 6000
caagtgtggt tcccagattc tgcaaccaag acagctcagg cattattaga cttcaaccgt 6060
gaaggattgc ctctgtttat cctggctaac tggagaggtt tctctggtgg acagagagat 6120
ctctttgaag gaattcttca ggctgggtca acaattgtcg agaaccttag gacatataat 6180
cagcctgcgt ttgtctacat tcctatggct ggagagcttc gtggaggagc ttgggttgtg 6240
gtcgatagca aaataaatcc agaccgcatt gagtgttatg ctgagaggac tgccaaaggt 6300
aatgttctcg aacctcaagg gttaattgaa atcaagttca ggtcagagga actccaagac 6360
tgtatgggta ggcttgaccc cgagttgata aatctgaaag caaaactcca agatgtaaag 6420
catggaaatg gaagtctacc agacatagaa tcccttcaga agagtataga agcacgtacg 6480
aaacagttgc tgcctttata tacccagatt gcaatacggt ttgctgaatt gcatgatact 6540
tccctaagaa tggcagctaa aggcgtgatt aagaaagttg tagactggga agaatcacgc 6600
tctttcttct ataaaaggct acggagaagg atctctgaag atgttcttgc aaaagaaata 6660
agacatatag tcggtgacaa cttcactcac caatcagcaa tggagctcat caaggaatgg 6720
tacctggctt ctccagccac agcaggaagc actggatggg atgacgatga tgcatttgtt 6780
gcctggaagg acagtcctga aaactacaat ggatatatcc aagagctaag ggctcaaaaa 6840
gtgtctcagt cgctctctga tctcactgac tccagttcag atctacaagc attctcgcag 6900
ggtctttcta cgctattaga taagatggat ccctctcaaa gagcgaagtt tgttcaggaa 6960
gtcaagaagg tccttggttg a 6981
<210> 2
<211> 2326
<212> PRT
<213> Sorghum
<400> 2
Met Ser Gln Leu Gly Leu Ala Ala Ala Ala Ser Lys Ala Leu Pro Leu
1 5 10 15
Leu Pro Asn Arg Gln Arg Ser Pro Ala Gly Thr Thr Phe Ser Ser Ser
20 25 30
Ala Leu Ser Arg Pro Ser Asn Arg Arg Lys Ser Arg Thr Arg Ser Leu
35 40 45
Arg Asp Gly Gly Asp Gly Val Ser Asp Val Lys Lys His Ser Gln Ser
50 55 60
Val Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Ser Glu Ala Ala
65 70 75 80
Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro Pro
85 90 95
Asp Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His Asn Gly Arg
100 105 110
His Ala Ser Val Ser Lys Val Val Glu Phe Cys Ala Ala Leu Gly Gly
115 120 125
Lys Thr Pro Ile His Ser Ile Leu Val Ala Asn Asn Gly Met Ala Ala
130 135 140
Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp Thr Phe Gly
145 150 155 160
Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met
165 170 175
Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val
180 185 190
Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val
195 200 205
Glu Val Ala Glu Arg Val Gly Val Ser Ala Val Trp Pro Gly Trp Gly
210 215 220
His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr Ala Lys Gly
225 230 235 240
Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met Asn Ala Leu Gly Asp
245 250 255
Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu
260 265 270
Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys Cys Leu Asp
275 280 285
Ala Ile Pro Glu Glu Met Tyr Arg Lys Ala Cys Val Thr Thr Thr Glu
290 295 300
Glu Ala Val Ala Ser Cys Gln Val Ile Gly Tyr Pro Ala Met Ile Lys
305 310 315 320
Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val His Asn Asp
325 330 335
Asp Glu Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly
340 345 350
Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg His Leu Glu
355 360 365
Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser
370 375 380
Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly
385 390 395 400
Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Ala Leu Glu Gln Ala
405 410 415
Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val
420 425 430
Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu
435 440 445
Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu
450 455 460
Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu
465 470 475 480
Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Tyr Gly Gly
485 490 495
Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn
500 505 510
Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val
515 520 525
Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly
530 535 540
Lys Val Lys Glu Ile Thr Phe Lys Ala Lys Pro Asn Val Trp Ala Tyr
545 550 555 560
Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln
565 570 575
Phe Gly His Val Phe Ala Tyr Gly Leu Ser Arg Ser Ala Ala Ile Thr
580 585 590
Asn Met Ala Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His
595 600 605
Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Arg
610 615 620
Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile Ala Met Arg
625 630 635 640
Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala
645 650 655
Leu Tyr Lys Thr Val Thr Thr Asn Ala Ala Thr Val Ser Asp Tyr Val
660 665 670
Ser Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val
675 680 685
Asn Ser Thr Val Ser Leu Asn Ile Glu Gly Ser Lys Tyr Thr Ile Glu
690 695 700
Thr Val Arg Thr Gly His Gly Ser Tyr Arg Leu Arg Met Asn Glu Ser
705 710 715 720
Thr Val Glu Ala Asn Val Gln Ser Leu Cys Asp Gly Gly Leu Leu Met
725 730 735
Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly
740 745 750
Gly Thr Arg Leu Gln Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp
755 760 765
His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg
770 775 780
Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val Pro Tyr Ala
785 790 795 800
Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ser
805 810 815
Gly Val Val His Cys Met Met Ser Glu Gly Gln Ala Leu Gln Ala Gly
820 825 830
Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg
835 840 845
Ala Glu Pro Phe Asp Gly Ile Phe Pro Gln Met Ala Leu Pro Val Ala
850 855 860
Ala Ser Ser Gln Val His Lys Arg Tyr Ala Ala Ser Leu Asn Ala Ala
865 870 875 880
Arg Met Val Leu Ala Gly Tyr Glu His Asn Ile Asn Glu Val Val Gln
885 890 895
Asp Leu Ile Cys Cys Leu Asp Asn Pro Glu Leu Pro Phe Leu Gln Trp
900 905 910
Asp Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Asn Leu Lys
915 920 925
Ser Glu Leu Glu Asp Lys Tyr Lys Glu Tyr Lys Leu Asn Phe Tyr Arg
930 935 940
Gly Lys Asn Val Asp Phe Pro Ser Lys Leu Leu Arg Asp Ile Ile Glu
945 950 955 960
Glu Asn Leu Ala Tyr Gly Ser Glu Lys Glu Lys Ala Thr Asn Glu Arg
965 970 975
Leu Val Glu Pro Leu Thr Asn Leu Leu Lys Ser Tyr Glu Gly Gly Arg
980 985 990
Glu Ser His Ala His Phe Ile Ala Lys Ser Leu Phe Glu Glu Tyr Leu
995 1000 1005
Met Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu
1010 1015 1020
Thr Leu Arg His Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile
1025 1030 1035 1040
Val Leu Ser His Gln Gly Val Arg Asn Lys Ala Lys Leu Val Thr Ala
1045 1050 1055
Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Val Ala Tyr Arg Asp Leu
1060 1065 1070
Leu Val Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala
1075 1080 1085
Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg
1090 1095 1100
Ala Ser Ile Ala Arg Ser Leu Ser Asp Leu Gly Met His Lys Gly Asp
1105 1110 1115 1120
Met Thr Ile Lys Asp Ser Met Glu Asp Leu Val Ser Ala Pro Leu Pro
1125 1130 1135
Val Glu Asp Ala Leu Ile Ser Leu Phe Asp Tyr Ser Asp Ala Thr Val
1140 1145 1150
Gln Gln Lys Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro His
1155 1160 1165
Leu Val Lys Asp Ser Ile Gln Met Lys Phe Lys Glu Tyr Gly Ala Ile
1170 1175 1180
Ala Phe Trp Glu Phe Ser Glu Gly His Val Asp Thr Ser Asn Gly His
1185 1190 1195 1200
Gly Thr Ile Leu Gly Gly Lys Arg Trp Gly Ser Met Val Val Leu Lys
1205 1210 1215
Ser Leu Glu Ser Ala Ser Thr Ala Ile Val Ala Ala Leu Lys Asp Ser
1220 1225 1230
Ala Gln Tyr Asn Ser Ser Glu Gly Asn Thr Met His Ile Ala Leu Leu
1235 1240 1245
Ser Ala Glu Asn Glu Ser Asn Met Ser Gly Thr Ser Asp Asp Gln Ala
1250 1255 1260
Gln His Arg Met Glu Lys Leu Thr Lys Ile Leu Lys Asp Thr Asn Val
1265 1270 1275 1280
Ala Ser Asp Leu Arg Ala Ala Gly Leu Lys Val Ile Ser Cys Ile Val
1285 1290 1295
Gln Arg Asp Glu Ala Arg Met Pro Met Arg His Thr Phe Leu Trp Leu
1300 1305 1310
Asp Glu Lys Ser Cys Tyr Glu Glu Glu Gln Ile Leu Arg His Val Glu
1315 1320 1325
Pro Pro Leu Ser Ala Leu Leu Glu Leu Asp Lys Leu Lys Val Lys Gly
1330 1335 1340
Tyr Asn Glu Met Lys Tyr Thr Pro Ser Arg Asp Arg Gln Trp His Ile
1345 1350 1355 1360
Tyr Thr Leu Arg Asn Thr Glu Asn Pro Lys Met Leu His Arg Val Phe
1365 1370 1375
Phe Arg Thr Ile Val Arg Gln Pro Asn Ala Gly Asn Lys Phe Thr Ser
1380 1385 1390
Ala Gln Val Ser Asp Thr Glu Val Gly Gly Pro Glu Asp Ser Leu Ser
1395 1400 1405
Phe Thr Ser Asn Ser Ile Leu Arg Ser Leu Met Thr Ala Ile Glu Glu
1410 1415 1420
Leu Glu Leu His Ala Ile Arg Thr Gly His Ser His Met Tyr Leu Cys
1425 1430 1435 1440
Ile Leu Lys Glu Gln Lys Leu Leu Asp Leu Ile Pro Phe Ser Gly Ser
1445 1450 1455
Thr Ile Val Asp Val Gly Gln Asp Glu Ala Thr Ala Cys Ser Leu Leu
1460 1465 1470
Lys Ser Met Ala Leu Lys Ile His Asp Leu Val Gly Ala Arg Met His
1475 1480 1485
His Leu Ser Val Cys Gln Trp Glu Val Lys Leu Lys Leu Asp Cys Asp
1490 1495 1500
Gly Pro Ala Ser Gly Thr Trp Arg Val Val Thr Thr Asn Val Thr Ser
1505 1510 1515 1520
His Thr Cys Thr Ile Asp Ile Tyr Arg Glu Val Glu Asp Thr Glu Ser
1525 1530 1535
Gln Lys Leu Val Tyr His Ser Thr Thr Ser Ala Ala Gly Pro Gly Pro
1540 1545 1550
Leu His Gly Val Ala Leu Asn Asn Pro Tyr Gln Pro Leu Ser Val Ile
1555 1560 1565
Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn Arg Thr Thr Tyr Cys Tyr
1570 1575 1580
Asp Phe Pro Leu Ala Phe Glu Thr Ala Leu Gln Lys Ser Trp Gln Ser
1585 1590 1595 1600
Asn Gly Ser Ser Val Ser Val Gly Ser Gly Asn Ser Lys Ser Tyr Val
1605 1610 1615
Lys Ala Thr Glu Leu Val Phe Ala Glu Lys His Gly Ser Trp Gly Thr
1620 1625 1630
Pro Ile Val Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met
1635 1640 1645
Val Ala Trp Ile Leu Glu Met Ser Thr Pro Glu Phe Pro Asn Gly Arg
1650 1655 1660
Gln Ile Ile Val Val Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe
1665 1670 1675 1680
Gly Pro Arg Glu Asp Ala Phe Phe Glu Ala Val Thr Asn Leu Ala Cys
1685 1690 1695
Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg
1700 1705 1710
Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser
1715 1720 1725
Asp Glu Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu
1730 1735 1740
Glu Asp Tyr Ala Arg Ile Ser Ser Ser Val Ile Ala His Lys Leu Gln
1745 1750 1755 1760
Leu Asp Ser Gly Glu Ile Arg Trp Ile Ile Asp Ser Val Val Gly Lys
1765 1770 1775
Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala
1780 1785 1790
Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val
1795 1800 1805
Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile
1810 1815 1820
Arg Cys Ile Gln Arg Leu Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser
1825 1830 1835 1840
Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln
1845 1850 1855
Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr
1860 1865 1870
Val Pro Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser
1875 1880 1885
Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Pro Leu
1890 1895 1900
Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp
1905 1910 1915 1920
Pro Arg Ala Ala Ile Arg Gly Val Asp Asp Ser Gln Gly Lys Trp Leu
1925 1930 1935
Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp
1940 1945 1950
Ala Lys Thr Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val
1955 1960 1965
Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Val Pro Ala
1970 1975 1980
Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly
1985 1990 1995 2000
Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Leu Leu
2005 2010 2015
Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg
2020 2025 2030
Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala
2035 2040 2045
Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe
2050 2055 2060
Val Tyr Ile Pro Met Ala Gly Glu Leu Arg Gly Gly Ala Trp Val Val
2065 2070 2075 2080
Val Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Cys Tyr Ala Glu Arg
2085 2090 2095
Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys
2100 2105 2110
Phe Arg Ser Glu Glu Leu Gln Asp Cys Met Gly Arg Leu Asp Pro Glu
2115 2120 2125
Leu Ile Asn Leu Lys Ala Lys Leu Gln Asp Val Lys His Gly Asn Gly
2130 2135 2140
Ser Leu Pro Asp Ile Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Thr
2145 2150 2155 2160
Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Ile Arg Phe Ala Glu
2165 2170 2175
Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys
2180 2185 2190
Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg
2195 2200 2205
Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg His Ile Val
2210 2215 2220
Gly Asp Asn Phe Thr His Gln Ser Ala Met Glu Leu Ile Lys Glu Trp
2225 2230 2235 2240
Tyr Leu Ala Ser Pro Ala Thr Ala Gly Ser Thr Gly Trp Asp Asp Asp
2245 2250 2255
Asp Ala Phe Val Ala Trp Lys Asp Ser Pro Glu Asn Tyr Asn Gly Tyr
2260 2265 2270
Ile Gln Glu Leu Arg Ala Gln Lys Val Ser Gln Ser Leu Ser Asp Leu
2275 2280 2285
Thr Asp Ser Ser Ser Asp Leu Gln Ala Phe Ser Gln Gly Leu Ser Thr
2290 2295 2300
Leu Leu Asp Lys Met Asp Pro Ser Gln Arg Ala Lys Phe Val Gln Glu
2305 2310 2315 2320
Val Lys Lys Val Leu Gly
2325
<210> 3
<211> 6981
<212> DNA
<213> Sorghum
<400> 3
atgtcgcagc ttggattagc tgcagctgcc tcaaaggcac tgccactact ccctaatcgc 60
cagcgaagtc ctgctgggac tacattctca tcatctgcat tgtcgaggcc ctcaaaccga 120
aggaaaagcc gtacccgttc actccgtgat ggtggagatg gggtatcaga tgtcaaaaag 180
cacagccagt ctgttcgtca aggtcttgct ggcattattg acctcccaag cgaggcagct 240
tcggaagtgg acatttcaca tggatctgag gatcctaggg gtccaccaga ttcctatcaa 300
atgaatggga ttatcaatga aacacataat ggaagacatg cttcagtgtc caaggttgtt 360
gaattttgtg cggcactagg tggcaaaaca ccaattcaca gtatattagt ggccaataat 420
ggaatggcag cagcaaagtt catgaggagt gtccggacat gggctaatga tacttttgga 480
tctgagaagg caattcagct catagctatg gcaactccgg aagacatgag gataaatgca 540
gagcacatta gaattgctga tcaatttgta gaggtgcctg gtggaacaaa caataataac 600
tacgccaacg ttcaactcat agtggaggta gcagaaagag taggtgtttc tgctgtttgg 660
cctggttggg gtcatgcttc tgagaatcct gaactgccgg atgcattgac tgcaaaagga 720
atcgttttcc ttgggccacc tgcatcatca atgaatgcat tgggagataa ggtcggttca 780
gctctcattg ctcaagcagc tggggtccca actcttgctt ggagtggatc acatgttgaa 840
gtgccattag agtgctgctt agacgctata cctgaggaga tgtatagaaa agcttgtgtt 900
actaccacag aggaagcagt tgcaagttgt caagtgattg gttatcctgc catgattaag 960
gcatcctggg gaggtggtgg taaaggaata agaaaggttc ataatgatga tgaggttaga 1020
gcactgttta agcaagtaca aggtgaagtc cctggctccc caatatttat catgaggctt 1080
gcagcccaga gtcggcatct tgaagttcag ttgctttgtg atcaatatgg caatgtagca 1140
gcacttcaca gtcgtgattg cagtgtgcaa cggcgacacc agaagattat tgaagaaggc 1200
ccagttactg ttgctcctcg tgagacagtt aaagcacttg agcaggcagc aaggaggctt 1260
gctaaggctg tgggttatgt tggtgctgct actgttgagt atctttacag catggaaact 1320
ggagaatact attttctgga gcttaatccc cgactacagg tcgagcatcc agtcaccgaa 1380
tggatagctg aagtaaatct gcctgcagct caagttgctg ttggaatggg catacctctt 1440
tggcaaattc cagaaatcag acgtttctat ggaatggact atggaggagg gtatgatatt 1500
tggaggaaaa cagcagctct tgctacacca tttaattttg atgaagtaga ttctcaatgg 1560
ccaaagggcc attgtgtagc agttagaatt actagtgagg accctgatga tggtttcaaa 1620
cctactggtg ggaaagtgaa ggagataact tttaaagcca agcctaatgt ttgggcctac 1680
ttctcagtaa agtctggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740
tttgcatatg gactctctag atcagcagca ataacaaaca tggctcttgc attaaaagag 1800
attcaaattc gtggagaaat tcattcaaat gttgattaca ctgttgacct cttaaacgct 1860
tcagacttca gagaaaacaa gattcatact ggttggctgg ataccagaat agctatgcgt 1920
gttcaagctg agaggccccc atggtatatt tcagtggttg gaggtgcttt atacaaaaca 1980
gtaaccacca atgcagccac tgtttctgac tatgttagtt atctcaccaa gggccagatt 2040
ccaccaaagc atatatccct tgtcaattct acagttagct tgaatataga agggagcaaa 2100
tacacaattg aaactgtaag gactggacat ggtagctaca ggttgcgaat gaatgaatcg 2160
acagttgaag cgaatgtaca atctttatgt gatggtggac tcttaatgca gttggatgga 2220
aacagccatg taatttatgc agaagaagaa gctggtggta cacggcttca gattgatgga 2280
aagacatgct tgttgcagaa tgaccatgat ccatcaaagt tattagctga gacaccctgc 2340
aaacttcttc gtttcttggt tgctgatggt gctcatgttg atgcggatgt accatacgct 2400
gaagttgagg ttatgaagat gtgcatgcct ctcctgtcac ctgcttctgg tgtcgttcat 2460
tgtatgatgt ctgagggcca ggcgttgcag gctggtgatc ttatagcaag gctggatctt 2520
gatgaccctt ctgctgtgaa aagggctgaa ccatttgatg gaatatttcc acaaatggcg 2580
ctccctgttg ctgcctctag tcaagtacac aaaagatatg ctgcaagttt gaatgctgct 2640
cgaatggttc ttgcaggata tgagcacaat atcaatgaag tcgttcaaga tttgatatgc 2700
tgcctggaca accctgagct tcctttccta cagtgggatg aacttatgtc tgttctagca 2760
acgaggcttc caagaaatct caagagtgag ttagaggata aatacaagga atacaagttg 2820
aatttttacc gtggaaaaaa cgtggacttc ccttccaagt tgctaagaga catcattgag 2880
gaaaatcttg catatggttc agagaaggaa aaggctacaa atgagaggct tgttgagcct 2940
cttacgaacc tactgaagtc atatgagggt gggagagaaa gtcatgcaca ttttattgcc 3000
aagtcccttt tcgaggagta tcttatggtg gaagaacttt tcagtgatgg aattcagtct 3060
gacgttattg aaaccttgcg tcatcagcac agtaaagacc tgcagaaggt tgtagacatt 3120
gtgttgtctc accagggtgt gagaaacaaa gctaagcttg taacagcact catggaaaag 3180
ctggtttatc caaatccggt tgcttacagg gatctgttgg ttcgcttttc ttccctcaat 3240
cataaaagat attataagtt ggcccttaaa gcaagtgaac ttcttgaaca aaccaaacta 3300
agtgaactcc gtgcaagcat tgcaagaagc ctttcagatc tggggatgca taagggagac 3360
atgactatta aggatagcat ggaagattta gtctctgccc cattgcctgt tgaagatgct 3420
cttatttctt tgtttgatta cagcgatgca actgttcagc agaaagtgat tgagacatac 3480
atatcacgat tgtaccagcc tcatcttgtg aaggatagca tccaaatgaa attcaaggaa 3540
tatggtgcta ttgctttttg ggaattttct gaagggcatg ttgatacttc aaatggacat 3600
gggactattc ttggtgggaa gagatggggt tccatggtcg tcctcaaatc acttgaatct 3660
gcatcaacag ccattgtggc tgcattaaag gattcagcac agtacaacag ctctgagggc 3720
aacacgatgc acattgcatt attgagtgct gaaaatgaaa gtaatatgag tggaacaagt 3780
gatgatcaag ctcaacatag gatggaaaag cttaccaaga tactgaagga tactaatgtt 3840
gcaagtgatc tccgagctgc tggtttgaag gttataagtt gcattgttca aagagatgaa 3900
gctcgcatgc caatgcgcca cacattcctc tggttggatg aaaagagttg ttatgaagaa 3960
gagcaaattc tccggcatgt ggagcctccc ctctctgcac ttcttgaatt ggataagttg 4020
aaagtgaaag gatacaatga aatgaagtat actccgtcac gtgatcgtca atggcatatc 4080
tacacactaa gaaatactga aaaccccaaa atgttgcata gggtattttt ccgaactatt 4140
gtcaggcaac ccaatgcagg caacaagttt acatcagctc aggtcagtga tactgaagta 4200
ggaggtcctg aagattctct gtcattcaca tcgaatagca tcctaagatc attgatgact 4260
gctatagaag aattagagct tcatgcgatt aggacaggtc attctcacat gtatttgtgc 4320
atactgaaag agcaaaagct tcttgatctc attccatttt cagggagtac aattgttgat 4380
gttggccaag atgaagctac tgcttgttca cttttaaaat caatggcttt gaagatacat 4440
gaccttgttg gtgcaaggat gcatcatctg tctgtatgcc agtgggaggt gaaactcaag 4500
ttggattgtg atgggcctgc aagtggtacc tggagagttg tgactacaaa tgttactagt 4560
cacacctgca ccattgatat ctaccgagaa gtggaagata cagaatcgca gaagttagta 4620
taccattcaa ccacttcggc agctggtcct ggtccgttgc atggtgttgc actgaataat 4680
ccatatcaac ctttaagtgt gattgatcta aagcgctgct ctgctaggaa caacagaaca 4740
acatattgct acgattttcc gctggcattt gaaactgcac tgcagaagtc atggcagtcc 4800
aacggctcta gtgtttctgt aggcagtgga aatagtaaat cctacgtgaa ggcaactgag 4860
ctggtgtttg ctgaaaaaca tgggtcctgg ggcactccta tagttcccat ggaacgtcct 4920
gctgggctca atgacattgg tatggtcgct tggatcttgg agatgtcaac acctgaattt 4980
cccaatggca ggcagattat tgttgtagca aatgatatta ctttcagagc tggatcattc 5040
ggcccaaggg aagatgcatt ttttgaagct gtcaccaacc tggcttgtga aaggaaactt 5100
ccccttatat acttggcagc aaactctggt gctaggattg gcatagctga tgaagtaaaa 5160
tcttgcttcc gtgttgggtg gtctgacgaa ggcagccctg agcgagggtt tcagtacatc 5220
tatctgactg aagaagacta tgcccgtatt agctcttctg ttatagcaca taagctgcag 5280
ctagatagcg gtgaaattag gtggattatt gactctgttg tgggcaagga ggatgggctt 5340
ggtgttgaga acatacatgg aagtgctgct atcgccagtg cttattctag ggcatatgag 5400
gagacattta cacttacatt tgtgaccgga cggactgtag gaataggagc ttatcttgct 5460
agacttggta tacggtgcat acagcgtctt gaccagccga ttattttaac agggttttct 5520
gccctgaaca agctccttgg gcgggaagtg tacagctccc acatgcagct tggtggtcct 5580
aagatcatgg cgaccaatgg tgttgtccac ctgactgttc cagatgacct tgaaggtgtt 5640
tccaatatat tgaggtggct cagctatgtt cctgcaaaca ttggtggacc tcttcctatt 5700
accaaacctt tggaccctcc agacagacct gttgcataca tccctgagaa cacatgcgat 5760
ccacgtgcag ccatccgtgg tgtagatgac agccaaggga aatggttggg tggtatgttt 5820
gacaaagaca gctttgtgga gacatttgaa ggatgggcaa aaacagtggt tactggcaga 5880
gcaaagcttg gaggaattcc tgtgggtgtc atagctgtgg agacacagac catgatgcag 5940
cttgtccctg ctgatccagg tcagcttgat tcccatgagc gatccgttcc tcgggctgga 6000
caagtgtggt tcccagattc tgtaaccaag acagctcagg cattattaga cttcaaccgt 6060
gaaggattgc ctctgtttat cctggctaac tggagaggtt tctctggtgg acagagagat 6120
ctctttgaag gaattcttca ggctgggtca acaattgtcg agaaccttag gacatataat 6180
cagcctgcgt ttgtctacat tcctatggct ggagagcttc gtggaggagc ttgggttgtg 6240
gtcgatagca aaataaatcc agaccgcatt gagtgttatg ctgagaggac tgccaaaggt 6300
aatgttctcg aacctcaagg gttaattgaa atcaagttca ggtcagagga actccaagac 6360
tgtatgggta ggcttgaccc cgagttgata aatctgaaag caaaactcca agatgtaaag 6420
catggaaatg gaagtctacc agacatagaa tcccttcaga agagtataga agcacgtacg 6480
aaacagttgc tgcctttata tacccagatt gcaatacggt ttgctgaatt gcatgatact 6540
tccctaagaa tggcagctaa aggcgtgatt aagaaagttg tagactggga agaatcacgc 6600
tctttcttct ataaaaggct acggagaagg atctctgaag atgttcttgc aaaagaaata 6660
agacatatag tcggtgacaa cttcactcac caatcagcaa tggagctcat caaggaatgg 6720
tacctggctt ctccagccac agcaggaagc actggatggg atgacgatga tgcatttgtt 6780
gcctggaagg acagtcctga aaactacaat ggatatatcc aagagctaag ggctcaaaaa 6840
gtgtctcagt cgctctctga tctcactgac tccagttcag atctacaagc attctcgcag 6900
ggtctttcta cgctattaga taagatggat ccctctcaaa gagcgaagtt tgttcaggaa 6960
gtcaagaagg tccttggttg a 6981
<210> 4
<211> 2326
<212> PRT
<213> Sorghum
<400> 4
Met Ser Gln Leu Gly Leu Ala Ala Ala Ala Ser Lys Ala Leu Pro Leu
1 5 10 15
Leu Pro Asn Arg Gln Arg Ser Pro Ala Gly Thr Thr Phe Ser Ser Ser
20 25 30
Ala Leu Ser Arg Pro Ser Asn Arg Arg Lys Ser Arg Thr Arg Ser Leu
35 40 45
Arg Asp Gly Gly Asp Gly Val Ser Asp Val Lys Lys His Ser Gln Ser
50 55 60
Val Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Ser Glu Ala Ala
65 70 75 80
Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro Pro
85 90 95
Asp Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His Asn Gly Arg
100 105 110
His Ala Ser Val Ser Lys Val Val Glu Phe Cys Ala Ala Leu Gly Gly
115 120 125
Lys Thr Pro Ile His Ser Ile Leu Val Ala Asn Asn Gly Met Ala Ala
130 135 140
Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp Thr Phe Gly
145 150 155 160
Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met
165 170 175
Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val
180 185 190
Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val
195 200 205
Glu Val Ala Glu Arg Val Gly Val Ser Ala Val Trp Pro Gly Trp Gly
210 215 220
His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr Ala Lys Gly
225 230 235 240
Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met Asn Ala Leu Gly Asp
245 250 255
Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu
260 265 270
Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys Cys Leu Asp
275 280 285
Ala Ile Pro Glu Glu Met Tyr Arg Lys Ala Cys Val Thr Thr Thr Glu
290 295 300
Glu Ala Val Ala Ser Cys Gln Val Ile Gly Tyr Pro Ala Met Ile Lys
305 310 315 320
Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val His Asn Asp
325 330 335
Asp Glu Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly
340 345 350
Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg His Leu Glu
355 360 365
Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser
370 375 380
Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly
385 390 395 400
Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Ala Leu Glu Gln Ala
405 410 415
Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val
420 425 430
Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu
435 440 445
Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu
450 455 460
Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu
465 470 475 480
Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Tyr Gly Gly
485 490 495
Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn
500 505 510
Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val
515 520 525
Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly
530 535 540
Lys Val Lys Glu Ile Thr Phe Lys Ala Lys Pro Asn Val Trp Ala Tyr
545 550 555 560
Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln
565 570 575
Phe Gly His Val Phe Ala Tyr Gly Leu Ser Arg Ser Ala Ala Ile Thr
580 585 590
Asn Met Ala Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His
595 600 605
Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Arg
610 615 620
Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile Ala Met Arg
625 630 635 640
Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala
645 650 655
Leu Tyr Lys Thr Val Thr Thr Asn Ala Ala Thr Val Ser Asp Tyr Val
660 665 670
Ser Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val
675 680 685
Asn Ser Thr Val Ser Leu Asn Ile Glu Gly Ser Lys Tyr Thr Ile Glu
690 695 700
Thr Val Arg Thr Gly His Gly Ser Tyr Arg Leu Arg Met Asn Glu Ser
705 710 715 720
Thr Val Glu Ala Asn Val Gln Ser Leu Cys Asp Gly Gly Leu Leu Met
725 730 735
Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly
740 745 750
Gly Thr Arg Leu Gln Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp
755 760 765
His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg
770 775 780
Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val Pro Tyr Ala
785 790 795 800
Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ser
805 810 815
Gly Val Val His Cys Met Met Ser Glu Gly Gln Ala Leu Gln Ala Gly
820 825 830
Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg
835 840 845
Ala Glu Pro Phe Asp Gly Ile Phe Pro Gln Met Ala Leu Pro Val Ala
850 855 860
Ala Ser Ser Gln Val His Lys Arg Tyr Ala Ala Ser Leu Asn Ala Ala
865 870 875 880
Arg Met Val Leu Ala Gly Tyr Glu His Asn Ile Asn Glu Val Val Gln
885 890 895
Asp Leu Ile Cys Cys Leu Asp Asn Pro Glu Leu Pro Phe Leu Gln Trp
900 905 910
Asp Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Asn Leu Lys
915 920 925
Ser Glu Leu Glu Asp Lys Tyr Lys Glu Tyr Lys Leu Asn Phe Tyr Arg
930 935 940
Gly Lys Asn Val Asp Phe Pro Ser Lys Leu Leu Arg Asp Ile Ile Glu
945 950 955 960
Glu Asn Leu Ala Tyr Gly Ser Glu Lys Glu Lys Ala Thr Asn Glu Arg
965 970 975
Leu Val Glu Pro Leu Thr Asn Leu Leu Lys Ser Tyr Glu Gly Gly Arg
980 985 990
Glu Ser His Ala His Phe Ile Ala Lys Ser Leu Phe Glu Glu Tyr Leu
995 1000 1005
Met Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu
1010 1015 1020
Thr Leu Arg His Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile
1025 1030 1035 1040
Val Leu Ser His Gln Gly Val Arg Asn Lys Ala Lys Leu Val Thr Ala
1045 1050 1055
Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Val Ala Tyr Arg Asp Leu
1060 1065 1070
Leu Val Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala
1075 1080 1085
Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg
1090 1095 1100
Ala Ser Ile Ala Arg Ser Leu Ser Asp Leu Gly Met His Lys Gly Asp
1105 1110 1115 1120
Met Thr Ile Lys Asp Ser Met Glu Asp Leu Val Ser Ala Pro Leu Pro
1125 1130 1135
Val Glu Asp Ala Leu Ile Ser Leu Phe Asp Tyr Ser Asp Ala Thr Val
1140 1145 1150
Gln Gln Lys Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro His
1155 1160 1165
Leu Val Lys Asp Ser Ile Gln Met Lys Phe Lys Glu Tyr Gly Ala Ile
1170 1175 1180
Ala Phe Trp Glu Phe Ser Glu Gly His Val Asp Thr Ser Asn Gly His
1185 1190 1195 1200
Gly Thr Ile Leu Gly Gly Lys Arg Trp Gly Ser Met Val Val Leu Lys
1205 1210 1215
Ser Leu Glu Ser Ala Ser Thr Ala Ile Val Ala Ala Leu Lys Asp Ser
1220 1225 1230
Ala Gln Tyr Asn Ser Ser Glu Gly Asn Thr Met His Ile Ala Leu Leu
1235 1240 1245
Ser Ala Glu Asn Glu Ser Asn Met Ser Gly Thr Ser Asp Asp Gln Ala
1250 1255 1260
Gln His Arg Met Glu Lys Leu Thr Lys Ile Leu Lys Asp Thr Asn Val
1265 1270 1275 1280
Ala Ser Asp Leu Arg Ala Ala Gly Leu Lys Val Ile Ser Cys Ile Val
1285 1290 1295
Gln Arg Asp Glu Ala Arg Met Pro Met Arg His Thr Phe Leu Trp Leu
1300 1305 1310
Asp Glu Lys Ser Cys Tyr Glu Glu Glu Gln Ile Leu Arg His Val Glu
1315 1320 1325
Pro Pro Leu Ser Ala Leu Leu Glu Leu Asp Lys Leu Lys Val Lys Gly
1330 1335 1340
Tyr Asn Glu Met Lys Tyr Thr Pro Ser Arg Asp Arg Gln Trp His Ile
1345 1350 1355 1360
Tyr Thr Leu Arg Asn Thr Glu Asn Pro Lys Met Leu His Arg Val Phe
1365 1370 1375
Phe Arg Thr Ile Val Arg Gln Pro Asn Ala Gly Asn Lys Phe Thr Ser
1380 1385 1390
Ala Gln Val Ser Asp Thr Glu Val Gly Gly Pro Glu Asp Ser Leu Ser
1395 1400 1405
Phe Thr Ser Asn Ser Ile Leu Arg Ser Leu Met Thr Ala Ile Glu Glu
1410 1415 1420
Leu Glu Leu His Ala Ile Arg Thr Gly His Ser His Met Tyr Leu Cys
1425 1430 1435 1440
Ile Leu Lys Glu Gln Lys Leu Leu Asp Leu Ile Pro Phe Ser Gly Ser
1445 1450 1455
Thr Ile Val Asp Val Gly Gln Asp Glu Ala Thr Ala Cys Ser Leu Leu
1460 1465 1470
Lys Ser Met Ala Leu Lys Ile His Asp Leu Val Gly Ala Arg Met His
1475 1480 1485
His Leu Ser Val Cys Gln Trp Glu Val Lys Leu Lys Leu Asp Cys Asp
1490 1495 1500
Gly Pro Ala Ser Gly Thr Trp Arg Val Val Thr Thr Asn Val Thr Ser
1505 1510 1515 1520
His Thr Cys Thr Ile Asp Ile Tyr Arg Glu Val Glu Asp Thr Glu Ser
1525 1530 1535
Gln Lys Leu Val Tyr His Ser Thr Thr Ser Ala Ala Gly Pro Gly Pro
1540 1545 1550
Leu His Gly Val Ala Leu Asn Asn Pro Tyr Gln Pro Leu Ser Val Ile
1555 1560 1565
Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn Arg Thr Thr Tyr Cys Tyr
1570 1575 1580
Asp Phe Pro Leu Ala Phe Glu Thr Ala Leu Gln Lys Ser Trp Gln Ser
1585 1590 1595 1600
Asn Gly Ser Ser Val Ser Val Gly Ser Gly Asn Ser Lys Ser Tyr Val
1605 1610 1615
Lys Ala Thr Glu Leu Val Phe Ala Glu Lys His Gly Ser Trp Gly Thr
1620 1625 1630
Pro Ile Val Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met
1635 1640 1645
Val Ala Trp Ile Leu Glu Met Ser Thr Pro Glu Phe Pro Asn Gly Arg
1650 1655 1660
Gln Ile Ile Val Val Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe
1665 1670 1675 1680
Gly Pro Arg Glu Asp Ala Phe Phe Glu Ala Val Thr Asn Leu Ala Cys
1685 1690 1695
Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg
1700 1705 1710
Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser
1715 1720 1725
Asp Glu Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu
1730 1735 1740
Glu Asp Tyr Ala Arg Ile Ser Ser Ser Val Ile Ala His Lys Leu Gln
1745 1750 1755 1760
Leu Asp Ser Gly Glu Ile Arg Trp Ile Ile Asp Ser Val Val Gly Lys
1765 1770 1775
Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala
1780 1785 1790
Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val
1795 1800 1805
Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile
1810 1815 1820
Arg Cys Ile Gln Arg Leu Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser
1825 1830 1835 1840
Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln
1845 1850 1855
Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr
1860 1865 1870
Val Pro Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser
1875 1880 1885
Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Pro Leu
1890 1895 1900
Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp
1905 1910 1915 1920
Pro Arg Ala Ala Ile Arg Gly Val Asp Asp Ser Gln Gly Lys Trp Leu
1925 1930 1935
Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp
1940 1945 1950
Ala Lys Thr Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val
1955 1960 1965
Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Val Pro Ala
1970 1975 1980
Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly
1985 1990 1995 2000
Gln Val Trp Phe Pro Asp Ser Val Thr Lys Thr Ala Gln Ala Leu Leu
2005 2010 2015
Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg
2020 2025 2030
Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala
2035 2040 2045
Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe
2050 2055 2060
Val Tyr Ile Pro Met Ala Gly Glu Leu Arg Gly Gly Ala Trp Val Val
2065 2070 2075 2080
Val Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Cys Tyr Ala Glu Arg
2085 2090 2095
Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys
2100 2105 2110
Phe Arg Ser Glu Glu Leu Gln Asp Cys Met Gly Arg Leu Asp Pro Glu
2115 2120 2125
Leu Ile Asn Leu Lys Ala Lys Leu Gln Asp Val Lys His Gly Asn Gly
2130 2135 2140
Ser Leu Pro Asp Ile Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Thr
2145 2150 2155 2160
Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Ile Arg Phe Ala Glu
2165 2170 2175
Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys
2180 2185 2190
Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg
2195 2200 2205
Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg His Ile Val
2210 2215 2220
Gly Asp Asn Phe Thr His Gln Ser Ala Met Glu Leu Ile Lys Glu Trp
2225 2230 2235 2240
Tyr Leu Ala Ser Pro Ala Thr Ala Gly Ser Thr Gly Trp Asp Asp Asp
2245 2250 2255
Asp Ala Phe Val Ala Trp Lys Asp Ser Pro Glu Asn Tyr Asn Gly Tyr
2260 2265 2270
Ile Gln Glu Leu Arg Ala Gln Lys Val Ser Gln Ser Leu Ser Asp Leu
2275 2280 2285
Thr Asp Ser Ser Ser Asp Leu Gln Ala Phe Ser Gln Gly Leu Ser Thr
2290 2295 2300
Leu Leu Asp Lys Met Asp Pro Ser Gln Arg Ala Lys Phe Val Gln Glu
2305 2310 2315 2320
Val Lys Lys Val Leu Gly
2325

Claims (13)

1.一组抗除草剂植物的ACCase的突变体的氨基酸序列，与其野生型相比，带有A2008V的突变。
2.一组抗除草剂高粱的ACCase的突变体的氨基酸序列，与其野生型相比，带有A2008V的突变。
3.权利要求1或者2所述的氨基酸序列如SEQ ID NO：4所示。
4.一组抗除草剂植物的ACCase的突变体的核酸序列， 其编码权利要求1或者2所述的氨基酸序列。
5.一组抗除草剂高粱的ACCase的突变体的核酸序列， 其编码权利要求1或者2所述的氨基酸序列。
6.权利要求4或5所述的核酸序列如SEQ ID NO：3所示。
7.获得具有除草剂抗性的植物的方法，其包括如下步骤 ： （1）使植物包含权利要求4~6 所述的核酸 ； 或，（2）使植物表达权利要求 1~3 所述的蛋白质。
8.获得具有除草剂抗性的高粱的方法，其包括如下步骤 ： （1）使高粱包含权利要求4~6 所述的核酸 ； 或，（2）使高粱表达权利要求 1~3 所述的蛋白质。
9.鉴定具有除草剂抗性的植物的方法，其包括如下步骤 ： （1）测试植物是否包含权利要求4~6 所述的核酸 ； 或，（2）测试植物是否表达权利要求 1~3 所述的蛋白质。
10.鉴定具有除草剂抗性的高粱的方法，其包括如下步骤 ： （1）测试高粱是否包含权利要求4~6 所述的核酸 ； 或，（2）测试高粱是否表达权利要求 1~3 所述的蛋白质。
11.权利要求1~3所述的蛋白质、权利要求4~6所述的核酸在获得抗除草剂的植物中的应用。
12.权利要求 1 所述的除草剂是ACCase抑制剂类除草剂。
13.权利要求 1 所述的除草剂是喹禾糠酯（其化学名称为(RS)-2-[4-(6-氯喹喔啉-2-氧基)苯氧基]丙酸-2-四氢呋喃甲基酯，其CAS号为119738-06-6）。
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